pycom13g02870

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005622
Physical Location & Seq
Reverse (-)
1961012 .. 1966278
5267 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2517 bp
ATGCCCTCTTCTTTCCTCCTCCTCCTCCACCCATCTCCTCCGTCTCTGCAATTCCCTCACGCGTCGTCGCCGTCGCCTTCACATCCCAAACTGATTGCCTCCTCCTCCAAACCCTCACTCCTGGCGATGAAGGATCGGCCTCCTTCCTCGTACGGCTCCGTCTACGTCCCTCCCCACCACCGCCTCCGCTCCGCCATTACCACCCCCAATTACACCTCCCCTACTTCAGTCGGCTCCAAGCTCCGCGACAACCAGACTGCTGCTGCTCTCAACCAGAGGAGCAGCACCAATGGTGTTCTGGCGTACTACCAGACTCAGCAGCAGCAGTTTCAGAAGCCCAAGCTTCAGCACTCTTCCGCTTACGACGACGGCGTTTCCGAGGAAGGCTCCGATCGCGAAGCTGAGTTGCCGTCGCATCCGACACAGAGTCCTTACTCATCTGATAATGAGTGGGAACGGAAATTAACCATGCTGTTATGTGACAAGGAGAAGCAAGAGTTGGTTACAAGGGAGAAAAAAGATAGATGCAACTTTGAGAAAATAGCTGCTTTGGCAAGCAGAATGGGGCTTTATAGCCATCTGTATTCGAAAGTTGCTGTGTTTAGTAAGGTCCCACTGCCAAACTACAGATTTGACCTGGATGATAGGCGCCCTCAGAGAGAGGTGACCTTGCCTCTTGGGTTGCTGAGGAGAGTTGAGGGATATCTTGGAGAGTTCCTTTCCCAGAAGTCTAGGACAAAGGAAGATCTCTCCTTTTCTACATCAAACAGTAGTGGCAGCATTGCTACTGACGAAGGGCTTTTTGAGCAACCAGAATCATTTGCGTCAAGTAAGGGTGTGATGGAAAAACTTCTTTGGCGGAGAAGTATGCAGCTGCATGATAAGCAACAAGCTTGGCAGGAATCTCCCGAAGGTAGAAAAATGCTTGAACTTCGCAGAAGTCTTCCGGCCTATAGAGAGAAGGATGCATTATTGGCAGCAATTTCACGAGATCAGGTTGTTATCATATCAGGTGAAACTGGCTGTGGCAAGACTACTCAAATTCCACAATTCATTTTAGAATCTGAGATAGAAGCTGTTCGTGGAGCTGTTTGTAGTATTATATGTACACAGCCTAGACGAATATCTGCTATGTCTGTTTCTGAAAGAGTTGCTTCAGAGAGAGGGGAGAAATTGGGTGACTCGGTTGGGTATAAGGTTCGGTTAGAAGGTATGAAGGGGAGGGACACTCACCTTCTCTTTTGCACCACTGGCATTTTGTTGAGAAGATTACTAGTTGATAGAAATTTGAAAGGTATATCTCATGTGATTGTGGATGAAATTCATGAACGTGGAATGAATGAAGATTTTCTGCTTATTGTTCTCAAGGATCTCCTTCCTCTGCGACCAGAACTTAGGCTGATTTTGATGAGTGCAACCCTAGATTCTGAGCTTTTCTCATCCTACTTTGGTAGAGCTCCGATAATTCATGTTCCAGGTTTTACATATCCAGTTCGAACTCATTTTCTGGAGGATGTTCTGGAAATTACGGGATGCAAATTAACGCCATACAATCAGATTGATGATTATGGTCAAGAAAAGATGTGGAAAATGAGCAAACAGGCGCCAAGAAAGAGGAAAAGCCAAATTGCTTCTGTTGTTGAGGATGCACTTAAAGCAGCCGATTTTAAGGAGTATAGTCCGGAGACTCGGGAGTCTCTAGCATGTTGGAATCCTGATAGTATCGGTTTTTATCTCATCGAGTATCTCTTGTGCAATATCTGTGAGAGTGACAAGCCTGGTGCCATTCTAGTTTTTATGACTGGGTGGGATGACATCAATTCTCTGAAGGAAAAGTTACATGCCAATCCTCTATTAGGTGATCCAAGCCAAGTCTTGTTACTAGCATGCCATGGTTCTATGGCAAGTACTGAGCAGAGGTTAATATTTGATGAGCCTGAGGATGGAGTGAGGAAGATAGTGCTGGCTACTAATATTGCTGAGACAAGCATCACGATTAATGATGTTGTTTTTGTACTTGATTGTGGAAAGGCAAAAGAGACTTCATATGACGCACTGAATAATACTCCTTGTTTGCTTCCTTCCTGGATTTCCAAGGTTTCTGCCCAACAAAGAAGAGGAAGAGCAGGACGTGTCCAGCCCGGGGAATGTTATCATCTCTATCCTAGATGTGTGTATGACGCATTTGCAGAGTATCAGTTACCGGAGATTTTGAGGACACCTCTGCAGTCTCTCTGTCTGCAAATAAAAAGTTTAAACCTCGGAAGCATTTCTGAGTTCTTATCTAGGGCTCTGCAGTCACCAGAGTTACTTGCGGTGCAAAACGCCATAGAGTATTTAAAGATCACTGGGGCCTTGGATGAGAAAGAGAATCTGACTGTTTTAGGACGCTATCTAACTATGCTTCCTGTGGAGCCAAAACTTGGAAAGATGCTCTTGCTAGGCGCCATTTTCAACTGCCTGGATCCAGTTTTGACTATTGTTTCTGGGCTTAGTGTCAGATCGATCCTTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

839

Amino Acids

93.87

Weight (kDa)

7.23

Isoelectric Point (pI)

54.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 325 - 477 3.9e-11 DEAD/DEAH box helicase
Helicase_C PF00271 584 - 712 1.5e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 776 - 804 3.9e-06 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 805 - 837 1.1e-09 Helicase associated domain (HA2), ratchet-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1693
AccB1I GGYRCC 4 cut(s) 648, 1603, 1781, 2444
AccB7I CCANNNNNTGG 1 cut(s) 2423
AccBSI CCGCTC 1 cut(s) 189
AccI GTMKAC 1 cut(s) 162
AccII CGCG 3 cut(s) 62, 246, 396
AccIII TCCGGA 1 cut(s) 1681
AciI CCGC 7 cut(s) 181, 187, 192, 244, 357, 859, 2315
AclWI GGATC 6 cut(s) 141, 1377, 1856, 2459, 2472, 2500
AcsI RAATTY 3 cut(s) 1041, 1285, 1320
AcuI CTGAAG 4 cut(s) 210, 329, 1140, 1847
AcyI GRCGYC 3 cut(s) 649, 1604, 2445
AfaI GTAC 5 cut(s) 152, 305, 1108, 1909, 2016
AfiI CCNNNNNNNGG 4 cut(s) 1856, 1943, 2143, 2423
AflIII ACRYGT 2 cut(s) 60, 2131
AgsI TTSAA 3 cut(s) 929, 1291, 2455
AhdI GACNNNNNGTC 1 cut(s) 426
AhlI ACTAGT 1 cut(s) 1273
AjiI CACGTC 1 cut(s) 2132
AjnI CCWGG 6 cut(s) 120, 636, 1474, 1777, 2084, 2460
AjuI GAANNNNNNNTTGG 2 cut(s) 482, 514
Alw21I GWGCWC 1 cut(s) 1459
Alw26I GTCTC 6 cut(s) 48, 1679, 1701, 1976, 2033, 2235
AlwI GGATC 6 cut(s) 141, 1377, 1856, 2459, 2472, 2500
Ama87I CYCGRG 2 cut(s) 1689, 2141
Aor13HI TCCGGA 1 cut(s) 1681
AoxI GGCC 3 cut(s) 137, 948, 2352
ApoI RAATTY 3 cut(s) 1041, 1285, 1320
AseI ATTAAT 1 cut(s) 1998
Asp700I GAANNNNTTC 4 cut(s) 849, 1077, 1347, 2269
AspLEI GCGC 3 cut(s) 651, 1606, 2447
AspS9I GGNCC 2 cut(s) 610, 2352
AsuC2I CCSGG 2 cut(s) 2142, 2143
AsuHPI GGTGA 6 cut(s) 676, 1025, 1190, 1223, 1871, 2292
AsuII TTCGAA 2 cut(s) 587, 1495
AvaI CYCGRG 2 cut(s) 1689, 2141
AvaII GGWCC 1 cut(s) 610
AxyI CCTNAGG 1 cut(s) 1938
BamHI GGATCC 1 cut(s) 2464
BanI GGYRCC 4 cut(s) 648, 1603, 1781, 2444
BanII GRGCYC 2 cut(s) 1459, 2293
BauI CACGAG 1 cut(s) 987
BbsI GAAGAC 1 cut(s) 935
Bbv12I GWGCWC 1 cut(s) 1459
BbvCI CCTCAGC 1 cut(s) 686
BccI CCATC 4 cut(s) 40, 585, 835, 1937
BceAI ACGGC 4 cut(s) 55, 169, 385, 394
BciT130I CCWGG 6 cut(s) 122, 638, 1476, 1779, 2086, 2462
BcnI CCSGG 2 cut(s) 2142, 2143
BcoDI GTCTC 6 cut(s) 48, 1679, 1701, 1976, 2033, 2235
BcuI ACTAGT 1 cut(s) 1273
BfmI CTRYAG 4 cut(s) 625, 952, 2225, 2294
BfoI RGCGCY 3 cut(s) 652, 1607, 2448
BglII AGATCT 1 cut(s) 745
BmcAI AGTACT 1 cut(s) 1909
Bme1390I CCNGG 8 cut(s) 122, 638, 1476, 1779, 2086, 2142, 2143, 2462
Bme18I GGWCC 1 cut(s) 610
BmeRI GACNNNNNGTC 1 cut(s) 426
BmeT110I CYCGRG 2 cut(s) 1689, 2141
BmgBI CACGTC 1 cut(s) 2132
BmgT120I GGNCC 2 cut(s) 610, 2352
BmrFI CCNGG 8 cut(s) 122, 638, 1476, 1779, 2086, 2142, 2143, 2462
BmrI ACTGGG 2 cut(s) 1812, 2358
BmsI GCATC 7 cut(s) 424, 515, 955, 1523, 1636, 1998, 2421
BmuI ACTGGG 2 cut(s) 1812, 2358
BpiI GAAGAC 1 cut(s) 935
BplI GAGNNNNNCTC 8 cut(s) 371, 403, 1213, 1245, 1421, 1453, 2206, 2238
BpmI CTGGAG 1 cut(s) 1529
Bpu10I CCTNAGC 1 cut(s) 686
Bpu14I TTCGAA 2 cut(s) 587, 1495
BpuEI CTTGAG 1 cut(s) 1349
BpuMI CCSGG 2 cut(s) 2142, 2143
Bsa29I ATCGAT 1 cut(s) 2504
BsaHI GRCGYC 3 cut(s) 649, 1604, 2445
BsaJI CCNNGG 7 cut(s) 378, 1891, 2094, 2141, 2142, 2260, 2355
BsaWI WCCGGW 2 cut(s) 1681, 2203
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bsc4I CCNNNNNNNGG 4 cut(s) 1856, 1943, 2143, 2423
Bse1I ACTGG 6 cut(s) 1024, 1255, 1490, 1807, 2353, 2468
Bse21I CCTNAGG 1 cut(s) 1938
Bse3DI GCAATG 1 cut(s) 780
BseAI TCCGGA 1 cut(s) 1681
BseBI CCWGG 6 cut(s) 122, 638, 1476, 1779, 2086, 2462
BseCI ATCGAT 1 cut(s) 2504
BseDI CCNNGG 7 cut(s) 378, 1891, 2094, 2141, 2142, 2260, 2355
BseLI CCNNNNNNNGG 4 cut(s) 1856, 1943, 2143, 2423
BseMI GCAATG 1 cut(s) 780
BseNI ACTGG 6 cut(s) 1024, 1255, 1490, 1807, 2353, 2468
BseRI GAGGAG 8 cut(s) 8, 11, 14, 27, 91, 94, 292, 703
Bsh1236I CGCG 3 cut(s) 62, 246, 396
Bsh1285I CGRYCG 1 cut(s) 394
BshFI GGCC 3 cut(s) 139, 950, 2354
BshNI GGYRCC 4 cut(s) 648, 1603, 1781, 2444
BshVI ATCGAT 1 cut(s) 2504
BsiEI CGRYCG 1 cut(s) 394
BsiHKAI GWGCWC 1 cut(s) 1459
BsiHKCI CYCGRG 2 cut(s) 1689, 2141
BsiSI CCGG 4 cut(s) 947, 1682, 2142, 2204
BsiWI CGTACG 1 cut(s) 150
BslFI GGGAC 3 cut(s) 152, 596, 1238
BslI CCNNNNNNNGG 4 cut(s) 1856, 1943, 2143, 2423
BsmAI GTCTC 6 cut(s) 48, 1679, 1701, 1976, 2033, 2235
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 3 cut(s) 152, 596, 1238
BsnI GGCC 3 cut(s) 139, 950, 2354
BsoBI CYCGRG 2 cut(s) 1689, 2141
Bsp119I TTCGAA 2 cut(s) 587, 1495
Bsp1286I GDGCHC 2 cut(s) 1459, 2293
Bsp13I TCCGGA 1 cut(s) 1681
Bsp1407I TGTACA 1 cut(s) 1106
Bsp19I CCATGG 1 cut(s) 1891
Bsp68I TCGCGA 1 cut(s) 396
BspACI CCGC 7 cut(s) 181, 187, 192, 244, 357, 859, 2315
BspANI GGCC 3 cut(s) 139, 950, 2354
BspDI ATCGAT 1 cut(s) 2504
BspEI TCCGGA 1 cut(s) 1681
BspFNI CGCG 3 cut(s) 62, 246, 396
BspHI TCATGA 1 cut(s) 1324
BspMAI CTGCAG 2 cut(s) 2229, 2298
BspPI GGATC 6 cut(s) 141, 1377, 1856, 2459, 2472, 2500
BspQI GCTCTTC 1 cut(s) 2116
BspT104I TTCGAA 2 cut(s) 587, 1495
BspT107I GGYRCC 4 cut(s) 648, 1603, 1781, 2444
BsrBI CCGCTC 1 cut(s) 189
BsrDI GCAATG 1 cut(s) 780
BsrGI TGTACA 1 cut(s) 1106
BsrI ACTGG 6 cut(s) 1024, 1255, 1490, 1807, 2353, 2468
BssECI CCNNGG 7 cut(s) 378, 1891, 2094, 2141, 2142, 2260, 2355
BssNI GRCGYC 3 cut(s) 649, 1604, 2445
BssSI CACGAG 1 cut(s) 987
BssT1I CCWWGG 3 cut(s) 1891, 2094, 2355
Bst2BI CACGAG 1 cut(s) 987
Bst2UI CCWGG 6 cut(s) 122, 638, 1476, 1779, 2086, 2462
Bst4CI ACNGT 2 cut(s) 770, 2380
Bst6I CTCTTC 4 cut(s) 13, 358, 2110, 2116
BstACI GRCGYC 3 cut(s) 649, 1604, 2445
BstAUI TGTACA 1 cut(s) 1106
BstBI TTCGAA 2 cut(s) 587, 1495
BstC8I GCNNGC 3 cut(s) 556, 1888, 1965
BstDSI CCRYGG 1 cut(s) 1891
BstEII GGTNACC 1 cut(s) 664
BstENI CCTNNNNNAGG 1 cut(s) 1854
BstFNI CGCG 3 cut(s) 62, 246, 396
BstH2I RGCGCY 3 cut(s) 652, 1607, 2448
BstHHI GCGC 3 cut(s) 651, 1606, 2447
BstMAI GTCTC 6 cut(s) 48, 1679, 1701, 1976, 2033, 2235
BstMCI CGRYCG 1 cut(s) 394
BstMWI GCNNNNNNNGC 7 cut(s) 393, 551, 805, 883, 974, 1251, 1655
BstNI CCWGG 6 cut(s) 122, 638, 1476, 1779, 2086, 2462
BstNSI RCATGY 3 cut(s) 1707, 1844, 1890
BstPI GGTNACC 1 cut(s) 664
BstSCI CCNGG 8 cut(s) 120, 636, 1474, 1777, 2084, 2140, 2141, 2460
BstSFI CTRYAG 4 cut(s) 625, 952, 2225, 2294
BstUI CGCG 3 cut(s) 62, 246, 396
BstV2I GAAGAC 1 cut(s) 935
BstX2I RGATCY 3 cut(s) 745, 1369, 2464
BstYI RGATCY 3 cut(s) 745, 1369, 2464
Bsu15I ATCGAT 1 cut(s) 2504
Bsu36I CCTNAGG 1 cut(s) 1938
BsuRI GGCC 3 cut(s) 139, 950, 2354
BsuTUI ATCGAT 1 cut(s) 2504
BtgI CCRYGG 1 cut(s) 1891
BtgZI GCGATG 1 cut(s) 140
BtrI CACGTC 1 cut(s) 2132
BtsI GCAGTG 1 cut(s) 614
BtsIMutI CAGTG 4 cut(s) 614, 1248, 2054, 2346
BtuMI TCGCGA 1 cut(s) 396
Cac8I GCNNGC 3 cut(s) 556, 1888, 1965
CciI TCATGA 1 cut(s) 1324
CfoI GCGC 3 cut(s) 651, 1606, 2447
Cfr13I GGNCC 2 cut(s) 610, 2352
Cfr9I CCCGGG 1 cut(s) 2141
ClaI ATCGAT 1 cut(s) 2504
CseI GACGC 5 cut(s) 51, 813, 2060, 2189, 2397
Csp6I GTAC 5 cut(s) 151, 304, 1107, 1908, 2015
CviAII CATG 9 cut(s) 469, 878, 1304, 1325, 1469, 1704, 1841, 1887, 1892
CviQI GTAC 5 cut(s) 151, 304, 1107, 1908, 2015
DinI GGCGCC 3 cut(s) 650, 1605, 2446
DraI TTTAAA 2 cut(s) 2256, 2340
DrdI GACNNNNNNGTC 1 cut(s) 1693
DriI GACNNNNNGTC 1 cut(s) 426
DseDI GACNNNNNNGTC 1 cut(s) 1693
Eam1104I CTCTTC 4 cut(s) 13, 358, 2110, 2116
Eam1105I GACNNNNNGTC 1 cut(s) 426
EarI CTCTTC 4 cut(s) 13, 358, 2110, 2116
EciI GGCGGA 2 cut(s) 181, 874
Ecl136II GAGCTC 1 cut(s) 1457
Eco130I CCWWGG 3 cut(s) 1891, 2094, 2355
Eco24I GRGCYC 2 cut(s) 1459, 2293
Eco32I GATATC 1 cut(s) 704
Eco47I GGWCC 1 cut(s) 610
Eco53kI GAGCTC 1 cut(s) 1457
Eco57I CTGAAG 4 cut(s) 210, 329, 1140, 1847
Eco81I CCTNAGG 1 cut(s) 1938
Eco88I CYCGRG 2 cut(s) 1689, 2141
Eco91I GGTNACC 1 cut(s) 664
EcoICRI GAGCTC 1 cut(s) 1457
EcoNI CCTNNNNNAGG 1 cut(s) 1854
EcoO109I RGGNCCY 2 cut(s) 610, 2352
EcoO65I GGTNACC 1 cut(s) 664
EcoRII CCWGG 6 cut(s) 120, 636, 1474, 1777, 2084, 2460
EcoRV GATATC 1 cut(s) 704
EcoT14I CCWWGG 3 cut(s) 1891, 2094, 2355
EcoT22I ATGCAT 1 cut(s) 970
EcoT38I GRGCYC 2 cut(s) 1459, 2293
EgeI GGCGCC 3 cut(s) 650, 1605, 2446
EheI GGCGCC 3 cut(s) 650, 1605, 2446
ErhI CCWWGG 3 cut(s) 1891, 2094, 2355
Esp3I CGTCTC 1 cut(s) 48
FaeI CATG 9 cut(s) 472, 881, 1307, 1328, 1472, 1707, 1844, 1890, 1895
FalI AAGNNNNNCTT 4 cut(s) 1138, 1170, 2420, 2452
FaqI GGGAC 3 cut(s) 152, 596, 1238
FatI CATG 9 cut(s) 468, 877, 1303, 1324, 1468, 1703, 1840, 1886, 1891
FauNDI CATATG 1 cut(s) 2047
FblI GTMKAC 1 cut(s) 162
FriOI GRGCYC 2 cut(s) 1459, 2293
GlaI GCGC 3 cut(s) 650, 1605, 2446
GsuI CTGGAG 1 cut(s) 1529
HaeII RGCGCY 3 cut(s) 652, 1607, 2448
HaeIII GGCC 3 cut(s) 139, 950, 2354
HapII CCGG 4 cut(s) 947, 1682, 2142, 2204
HgaI GACGC 5 cut(s) 51, 813, 2060, 2189, 2397
HhaI GCGC 3 cut(s) 651, 1606, 2447
Hin1I GRCGYC 3 cut(s) 649, 1604, 2445
Hin1II CATG 9 cut(s) 472, 881, 1307, 1328, 1472, 1707, 1844, 1890, 1895
Hin6I GCGC 3 cut(s) 649, 1604, 2445
HinP1I GCGC 3 cut(s) 649, 1604, 2445
HindIII AAGCTT 2 cut(s) 341, 891
HpaII CCGG 4 cut(s) 947, 1682, 2142, 2204
HphI GGTGA 6 cut(s) 676, 1025, 1190, 1223, 1871, 2292
Hpy166II GTNNAC 2 cut(s) 163, 1109
Hpy8I GTNNAC 2 cut(s) 163, 1109
Hpy99I CGWCG 6 cut(s) 67, 70, 76, 368, 371, 415
HpyCH4III ACNGT 2 cut(s) 770, 2380
HpyCH4IV ACGT 3 cut(s) 165, 1330, 2131
HpyF10VI GCNNNNNNNGC 7 cut(s) 393, 551, 805, 883, 974, 1251, 1655
HpySE526I ACGT 3 cut(s) 165, 1330, 2131
Hsp92I GRCGYC 3 cut(s) 649, 1604, 2445
Hsp92II CATG 9 cut(s) 472, 881, 1307, 1328, 1472, 1707, 1844, 1890, 1895
HspAI GCGC 3 cut(s) 649, 1604, 2445
KasI GGCGCC 3 cut(s) 648, 1603, 2444
Kpn2I TCCGGA 1 cut(s) 1681
LguI GCTCTTC 1 cut(s) 2116
LmnI GCTCC 9 cut(s) 161, 194, 239, 246, 279, 392, 1085, 1462, 2413
LweI GCATC 7 cut(s) 424, 515, 955, 1523, 1636, 1998, 2421
MaeII ACGT 3 cut(s) 165, 1330, 2131
MbiI CCGCTC 1 cut(s) 189
MboII GAAGA 8 cut(s) 345, 755, 935, 1278, 1355, 1966, 2127, 2133
MflI RGATCY 3 cut(s) 745, 1369, 2464
MhlI GDGCHC 2 cut(s) 1459, 2293
MluI ACGCGT 1 cut(s) 60
Mly113I GGCGCC 3 cut(s) 649, 1604, 2445
MlyI GAGTC 5 cut(s) 307, 436, 1175, 1681, 1703
MmeI TCCRAC 2 cut(s) 443, 1688
Mph1103I ATGCAT 1 cut(s) 970
MroI TCCGGA 1 cut(s) 1681
MroXI GAANNNNTTC 4 cut(s) 849, 1077, 1347, 2269
MseI TTAA 8 cut(s) 464, 1541, 1653, 1668, 1922, 1998, 2255, 2339
MslI CAYNNNNRTG 1 cut(s) 1329
MspA1I CMGCKG 1 cut(s) 874
MspI CCGG 4 cut(s) 947, 1682, 2142, 2204
MspR9I CCNGG 8 cut(s) 122, 638, 1476, 1779, 2086, 2142, 2143, 2462
MssI GTTTAAAC 1 cut(s) 2256
MvaI CCWGG 6 cut(s) 122, 638, 1476, 1779, 2086, 2462
MvnI CGCG 3 cut(s) 62, 246, 396
MwoI GCNNNNNNNGC 7 cut(s) 393, 551, 805, 883, 974, 1251, 1655
NarI GGCGCC 3 cut(s) 649, 1604, 2445
NciI CCSGG 2 cut(s) 2142, 2143
NcoI CCATGG 1 cut(s) 1891
NdeI CATATG 1 cut(s) 2047
NlaIII CATG 9 cut(s) 472, 881, 1307, 1328, 1472, 1707, 1844, 1890, 1895
NmuCI GTSAC 5 cut(s) 479, 664, 1178, 1769, 2298
NruI TCGCGA 1 cut(s) 396
NsiI ATGCAT 1 cut(s) 970
NspI RCATGY 3 cut(s) 1707, 1844, 1890
NspV TTCGAA 2 cut(s) 587, 1495
PaeI GCATGC 1 cut(s) 1890
PagI TCATGA 1 cut(s) 1324
PciSI GCTCTTC 1 cut(s) 2116
PcsI WCGNNNNNNNCGW 2 cut(s) 369, 375
PdmI GAANNNNTTC 4 cut(s) 849, 1077, 1347, 2269
PfeI GAWTC 6 cut(s) 815, 902, 1061, 1424, 1711, 2371
Pfl23II CGTACG 1 cut(s) 150
PflMI CCANNNNNTGG 1 cut(s) 2423
PfoI TCCNGGA 1 cut(s) 2084
Ple19I CGATCG 1 cut(s) 394
PleI GAGTC 5 cut(s) 307, 435, 1175, 1681, 1702
PluTI GGCGCC 3 cut(s) 652, 1607, 2448
PmeI GTTTAAAC 1 cut(s) 2256
PpsI GAGTC 5 cut(s) 307, 435, 1175, 1681, 1702
PpuMI RGGWCCY 1 cut(s) 610
PshBI ATTAAT 1 cut(s) 1998
Psp124BI GAGCTC 1 cut(s) 1459
Psp5II RGGWCCY 1 cut(s) 610
Psp6I CCWGG 6 cut(s) 120, 636, 1474, 1777, 2084, 2460
PspEI GGTNACC 1 cut(s) 664
PspGI CCWGG 6 cut(s) 120, 636, 1474, 1777, 2084, 2460
PspLI CGTACG 1 cut(s) 150
PspPI GGNCC 2 cut(s) 610, 2352
PspPPI RGGWCCY 1 cut(s) 610
PstI CTGCAG 2 cut(s) 2229, 2298
PsuI RGATCY 3 cut(s) 745, 1369, 2464
PvuI CGATCG 1 cut(s) 394
PvuII CAGCTG 1 cut(s) 874
RruI TCGCGA 1 cut(s) 396
RsaI GTAC 5 cut(s) 152, 305, 1108, 1909, 2016
RsaNI GTAC 5 cut(s) 151, 304, 1107, 1908, 2015
RseI CAYNNNNRTG 1 cut(s) 1329
SacI GAGCTC 1 cut(s) 1459
SapI GCTCTTC 1 cut(s) 2116
SaqAI TTAA 8 cut(s) 464, 1541, 1653, 1668, 1922, 1998, 2255, 2339
Sau96I GGNCC 2 cut(s) 610, 2352
ScaI AGTACT 1 cut(s) 1909
SchI GAGTC 5 cut(s) 307, 436, 1175, 1681, 1703
ScrFI CCNGG 8 cut(s) 122, 638, 1476, 1779, 2086, 2142, 2143, 2462
SduI GDGCHC 2 cut(s) 1459, 2293
SfaNI GCATC 7 cut(s) 424, 515, 955, 1523, 1636, 1998, 2421
SfcI CTRYAG 4 cut(s) 625, 952, 2225, 2294
SfoI GGCGCC 3 cut(s) 650, 1605, 2446
SfuI TTCGAA 2 cut(s) 587, 1495
SinI GGWCC 1 cut(s) 610
SmaI CCCGGG 1 cut(s) 2143
SmiMI CAYNNNNRTG 1 cut(s) 1329
SmlI CTYRAG 1 cut(s) 1364
SmoI CTYRAG 1 cut(s) 1364
SpeI ACTAGT 1 cut(s) 1273
SphI GCATGC 1 cut(s) 1890
SsiI CCGC 7 cut(s) 181, 187, 192, 244, 357, 859, 2315
SspDI GGCGCC 3 cut(s) 648, 1603, 2444
SspI AATATT 2 cut(s) 1926, 1975
SstI GAGCTC 1 cut(s) 1459
StyD4I CCNGG 8 cut(s) 120, 636, 1474, 1777, 2084, 2140, 2141, 2460
StyI CCWWGG 3 cut(s) 1891, 2094, 2355
TaaI ACNGT 2 cut(s) 770, 2380
TaiI ACGT 3 cut(s) 168, 1333, 2134
TaqI TCGA 4 cut(s) 587, 1495, 1740, 2504
TatI WGTACW 3 cut(s) 1106, 1907, 2014
TfiI GAWTC 6 cut(s) 815, 902, 1061, 1424, 1711, 2371
Tru1I TTAA 8 cut(s) 464, 1541, 1653, 1668, 1922, 1998, 2255, 2339
Tru9I TTAA 8 cut(s) 464, 1541, 1653, 1668, 1922, 1998, 2255, 2339
TscAI CASTG 4 cut(s) 621, 1255, 2061, 2353
TseFI GTSAC 5 cut(s) 479, 664, 1178, 1769, 2298
Tsp45I GTSAC 5 cut(s) 479, 664, 1178, 1769, 2298
TspGWI ACGGA 3 cut(s) 30, 148, 472
TspMI CCCGGG 1 cut(s) 2141
TspRI CASTG 4 cut(s) 621, 1255, 2061, 2353
Van91I CCANNNNNTGG 1 cut(s) 2423
VpaK11BI GGWCC 1 cut(s) 610
VspI ATTAAT 1 cut(s) 1998
XagI CCTNNNNNAGG 1 cut(s) 1854
XapI RAATTY 3 cut(s) 1041, 1285, 1320
XceI RCATGY 3 cut(s) 1707, 1844, 1890
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
XmaI CCCGGG 1 cut(s) 2141
XmiI GTMKAC 1 cut(s) 162
XmnI GAANNNNTTC 4 cut(s) 849, 1077, 1347, 2269
ZrmI AGTACT 1 cut(s) 1909
Zsp2I ATGCAT 1 cut(s) 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.