Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val)
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G13270 AT1G13270 |
| fragaria_vesca | FvH4_4g28020 |
| malus_domestica | MD13G1046000.v1.1 MD16G1047600.v1.1 |
| prunus_persica | Prupe.1G306000_v2.0.a1 Prupe.1G306000_v2.0.a1 Prupe.1G306000_v2.0.a1 Prupe.1G306000_v2.0.a1 |
| pyrus_communis | pycom13g04040 pycom16g04160 |
| rosa_chinensis | RchiOBHm_Chr4g0435761 |
| rosa_laevigata | RLG00000006561 |
| rosa_multiflora | Rmu_co8005306.1_g000001 Rmu_sc0000051.1_g000022 Rmu_sc0036818.1_g000001 |
| rosa_rugosa | Rorug04G0289400 |
| rosa_samantha | Rh4AG343700 Rh4BG352400 Rh4CG366900 Rh4DG346300 |
| rosa_wichuraiana | Rw4G030010 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1167 |
| AccB1I | GGYRCC | 1 cut(s) | 638 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 371 |
| AccI | GTMKAC | 1 cut(s) | 774 |
| AccII | CGCG | 2 cut(s) | 549, 1148 |
| AciI | CCGC | 4 cut(s) | 383, 887, 1146, 1148 |
| AcoI | YGGCCR | 1 cut(s) | 1121 |
| AcsI | RAATTY | 2 cut(s) | 717, 1209 |
| AcuI | CTGAAG | 1 cut(s) | 525 |
| AfeI | AGCGCT | 1 cut(s) | 974 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 170, 371, 452, 1096 |
| AflIII | ACRYGT | 1 cut(s) | 1220 |
| AgsI | TTSAA | 6 cut(s) | 26, 201, 913, 1061, 1082, 1175 |
| AhlI | ACTAGT | 1 cut(s) | 847 |
| AluBI | AGCT | 6 cut(s) | 161, 237, 358, 527, 538, 909 |
| AluI | AGCT | 6 cut(s) | 161, 237, 358, 527, 538, 909 |
| Alw21I | GWGCWC | 1 cut(s) | 618 |
| Alw26I | GTCTC | 4 cut(s) | 295, 341, 741, 789 |
| Alw44I | GTGCAC | 1 cut(s) | 614 |
| Aor51HI | AGCGCT | 1 cut(s) | 974 |
| AoxI | GGCC | 7 cut(s) | 9, 181, 321, 380, 440, 578, 1121 |
| ApaLI | GTGCAC | 1 cut(s) | 614 |
| ApeKI | GCWGC | 5 cut(s) | 174, 355, 527, 542, 1163 |
| ApoI | RAATTY | 2 cut(s) | 717, 1209 |
| AseI | ATTAAT | 1 cut(s) | 1019 |
| AspLEI | GCGC | 4 cut(s) | 364, 975, 1168, 1205 |
| AspS9I | GGNCC | 2 cut(s) | 10, 225 |
| AsuC2I | CCSGG | 1 cut(s) | 1160 |
| AsuHPI | GGTGA | 2 cut(s) | 642, 1022 |
| AsuNHI | GCTAGC | 1 cut(s) | 905 |
| AvaII | GGWCC | 1 cut(s) | 225 |
| BaeGI | GKGCMC | 2 cut(s) | 618, 987 |
| BanI | GGYRCC | 1 cut(s) | 638 |
| Bbv12I | GWGCWC | 1 cut(s) | 618 |
| BbvI | GCAGC | 5 cut(s) | 161, 367, 514, 529, 1150 |
| BccI | CCATC | 3 cut(s) | 893, 1094, 1145 |
| BceAI | ACGGC | 1 cut(s) | 565 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 513, 524, 547, 558 |
| BclI | TGATCA | 1 cut(s) | 427 |
| BcnI | CCSGG | 1 cut(s) | 1160 |
| BcoDI | GTCTC | 4 cut(s) | 295, 341, 741, 789 |
| BcuI | ACTAGT | 1 cut(s) | 847 |
| BfaI | CTAG | 4 cut(s) | 99, 498, 848, 906 |
| BfmI | CTRYAG | 1 cut(s) | 561 |
| BfoI | RGCGCY | 2 cut(s) | 976, 1206 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 391 |
| BisI | GCNGC | 6 cut(s) | 175, 356, 383, 528, 543, 1164 |
| BlsI | GCNGC | 6 cut(s) | 176, 357, 384, 529, 544, 1165 |
| Bme1390I | CCNGG | 1 cut(s) | 1160 |
| Bme18I | GGWCC | 1 cut(s) | 225 |
| BmgT120I | GGNCC | 2 cut(s) | 10, 225 |
| BmiI | GGNNCC | 4 cut(s) | 226, 227, 640, 1051 |
| BmrFI | CCNGG | 1 cut(s) | 1160 |
| BmrI | ACTGGG | 1 cut(s) | 7 |
| BmsI | GCATC | 2 cut(s) | 622, 1113 |
| BmtI | GCTAGC | 1 cut(s) | 909 |
| BmuI | ACTGGG | 1 cut(s) | 7 |
| BpuMI | CCSGG | 1 cut(s) | 1160 |
| BsaJI | CCNNGG | 5 cut(s) | 178, 437, 712, 1053, 1146 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 56, 86 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 170, 371, 452, 1096 |
| Bse118I | RCCGGY | 1 cut(s) | 1199 |
| Bse1I | ACTGG | 2 cut(s) | 13, 1042 |
| Bse3DI | GCAATG | 1 cut(s) | 510 |
| BseDI | CCNNGG | 5 cut(s) | 178, 437, 712, 1053, 1146 |
| BseGI | GGATG | 4 cut(s) | 829, 904, 1086, 1156 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 170, 371, 452, 1096 |
| BseMI | GCAATG | 1 cut(s) | 510 |
| BseMII | CTCAG | 2 cut(s) | 171, 247 |
| BseNI | ACTGG | 2 cut(s) | 13, 1042 |
| BseRI | GAGGAG | 1 cut(s) | 332 |
| BseSI | GKGCMC | 2 cut(s) | 618, 987 |
| BseXI | GCAGC | 5 cut(s) | 161, 367, 514, 529, 1150 |
| Bsh1236I | CGCG | 2 cut(s) | 549, 1148 |
| BshFI | GGCC | 7 cut(s) | 11, 183, 323, 382, 442, 580, 1123 |
| BshNI | GGYRCC | 1 cut(s) | 638 |
| BsiHKAI | GWGCWC | 1 cut(s) | 618 |
| BsiSI | CCGG | 4 cut(s) | 141, 1124, 1160, 1200 |
| BslFI | GGGAC | 2 cut(s) | 211, 1009 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 170, 371, 452, 1096 |
| BsmAI | GTCTC | 4 cut(s) | 295, 341, 741, 789 |
| BsmFI | GGGAC | 2 cut(s) | 211, 1009 |
| BsnI | GGCC | 7 cut(s) | 11, 183, 323, 382, 442, 580, 1123 |
| Bsp1286I | GDGCHC | 2 cut(s) | 618, 987 |
| Bsp143I | GATC | 1 cut(s) | 427 |
| Bsp19I | CCATGG | 1 cut(s) | 1053 |
| BspACI | CCGC | 4 cut(s) | 383, 887, 1146, 1148 |
| BspANI | GGCC | 7 cut(s) | 11, 183, 323, 382, 442, 580, 1123 |
| BspCNI | CTCAG | 2 cut(s) | 170, 246 |
| BspFNI | CGCG | 2 cut(s) | 549, 1148 |
| BspLI | GGNNCC | 4 cut(s) | 226, 227, 640, 1051 |
| BspMAI | CTGCAG | 1 cut(s) | 565 |
| BspOI | GCTAGC | 1 cut(s) | 909 |
| BspT107I | GGYRCC | 1 cut(s) | 638 |
| BsrDI | GCAATG | 1 cut(s) | 510 |
| BsrFI | RCCGGY | 1 cut(s) | 1199 |
| BsrI | ACTGG | 2 cut(s) | 13, 1042 |
| BssAI | RCCGGY | 1 cut(s) | 1199 |
| BssECI | CCNNGG | 5 cut(s) | 178, 437, 712, 1053, 1146 |
| BssMI | GATC | 1 cut(s) | 427 |
| BssT1I | CCWWGG | 2 cut(s) | 178, 1053 |
| Bst4CI | ACNGT | 6 cut(s) | 735, 772, 956, 989, 1000, 1034 |
| Bst6I | CTCTTC | 4 cut(s) | 90, 221, 671, 858 |
| BstC8I | GCNNGC | 7 cut(s) | 360, 525, 532, 536, 540, 547, 907 |
| BstDEI | CTNAG | 4 cut(s) | 157, 194, 233, 553 |
| BstDSI | CCRYGG | 3 cut(s) | 712, 1053, 1146 |
| BstEII | GGTNACC | 1 cut(s) | 856 |
| BstF5I | GGATG | 4 cut(s) | 829, 904, 1086, 1156 |
| BstFNI | CGCG | 2 cut(s) | 549, 1148 |
| BstH2I | RGCGCY | 2 cut(s) | 976, 1206 |
| BstHHI | GCGC | 4 cut(s) | 364, 975, 1168, 1205 |
| BstKTI | GATC | 1 cut(s) | 430 |
| BstMAI | GTCTC | 4 cut(s) | 295, 341, 741, 789 |
| BstMBI | GATC | 1 cut(s) | 427 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 180, 361, 391 |
| BstNSI | RCATGY | 3 cut(s) | 709, 944, 1224 |
| BstPI | GGTNACC | 1 cut(s) | 856 |
| BstSCI | CCNGG | 1 cut(s) | 1158 |
| BstSFI | CTRYAG | 1 cut(s) | 561 |
| BstSLI | GKGCMC | 2 cut(s) | 618, 987 |
| BstUI | CGCG | 2 cut(s) | 549, 1148 |
| BstV1I | GCAGC | 5 cut(s) | 161, 367, 514, 529, 1150 |
| BsuRI | GGCC | 7 cut(s) | 11, 183, 323, 382, 442, 580, 1123 |
| BtgI | CCRYGG | 3 cut(s) | 712, 1053, 1146 |
| BtsCI | GGATG | 4 cut(s) | 829, 904, 1086, 1156 |
| BtsI | GCAGTG | 1 cut(s) | 618 |
| BtsIMutI | CAGTG | 2 cut(s) | 618, 828 |
| Cac8I | GCNNGC | 7 cut(s) | 360, 525, 532, 536, 540, 547, 907 |
| CfoI | GCGC | 4 cut(s) | 364, 975, 1168, 1205 |
| Cfr10I | RCCGGY | 1 cut(s) | 1199 |
| Cfr13I | GGNCC | 2 cut(s) | 10, 225 |
| Cfr42I | CCGCGG | 1 cut(s) | 1149 |
| CseI | GACGC | 2 cut(s) | 538, 1147 |
| CviAII | CATG | 7 cut(s) | 170, 325, 706, 791, 941, 1054, 1221 |
| DdeI | CTNAG | 4 cut(s) | 157, 194, 233, 553 |
| DpnI | GATC | 1 cut(s) | 429 |
| DpnII | GATC | 1 cut(s) | 427 |
| EaeI | YGGCCR | 1 cut(s) | 1121 |
| Eam1104I | CTCTTC | 4 cut(s) | 90, 221, 671, 858 |
| EarI | CTCTTC | 4 cut(s) | 90, 221, 671, 858 |
| Eco130I | CCWWGG | 2 cut(s) | 178, 1053 |
| Eco147I | AGGCCT | 1 cut(s) | 442 |
| Eco32I | GATATC | 1 cut(s) | 788 |
| Eco47I | GGWCC | 1 cut(s) | 225 |
| Eco47III | AGCGCT | 1 cut(s) | 974 |
| Eco57I | CTGAAG | 1 cut(s) | 525 |
| Eco91I | GGTNACC | 1 cut(s) | 856 |
| EcoO109I | RGGNCCY | 1 cut(s) | 225 |
| EcoO65I | GGTNACC | 1 cut(s) | 856 |
| EcoRI | GAATTC | 1 cut(s) | 717 |
| EcoRV | GATATC | 1 cut(s) | 788 |
| EcoT14I | CCWWGG | 2 cut(s) | 178, 1053 |
| ErhI | CCWWGG | 2 cut(s) | 178, 1053 |
| FaeI | CATG | 7 cut(s) | 173, 328, 709, 794, 944, 1057, 1224 |
| FaqI | GGGAC | 2 cut(s) | 211, 1009 |
| FatI | CATG | 7 cut(s) | 169, 324, 705, 790, 940, 1053, 1220 |
| FbaI | TGATCA | 1 cut(s) | 427 |
| FblI | GTMKAC | 1 cut(s) | 774 |
| Fnu4HI | GCNGC | 6 cut(s) | 175, 356, 383, 528, 543, 1164 |
| FokI | GGATG | 4 cut(s) | 836, 911, 1073, 1163 |
| Fsp4HI | GCNGC | 6 cut(s) | 175, 356, 383, 528, 543, 1164 |
| FspBI | CTAG | 4 cut(s) | 99, 498, 848, 906 |
| FspI | TGCGCA | 1 cut(s) | 1167 |
| GlaI | GCGC | 4 cut(s) | 363, 974, 1167, 1204 |
| GluI | GCNGC | 6 cut(s) | 175, 356, 383, 528, 543, 1164 |
| HaeII | RGCGCY | 2 cut(s) | 976, 1206 |
| HaeIII | GGCC | 7 cut(s) | 11, 183, 323, 382, 442, 580, 1123 |
| HapII | CCGG | 4 cut(s) | 141, 1124, 1160, 1200 |
| HgaI | GACGC | 2 cut(s) | 538, 1147 |
| HhaI | GCGC | 4 cut(s) | 364, 975, 1168, 1205 |
| Hin1II | CATG | 7 cut(s) | 173, 328, 709, 794, 944, 1057, 1224 |
| Hin6I | GCGC | 4 cut(s) | 362, 973, 1166, 1203 |
| HinP1I | GCGC | 4 cut(s) | 362, 973, 1166, 1203 |
| HincII | GTYRAC | 2 cut(s) | 210, 697 |
| HindII | GTYRAC | 2 cut(s) | 210, 697 |
| HinfI | GANTC | 5 cut(s) | 263, 349, 557, 725, 833 |
| HpaI | GTTAAC | 1 cut(s) | 697 |
| HpaII | CCGG | 4 cut(s) | 141, 1124, 1160, 1200 |
| HphI | GGTGA | 2 cut(s) | 642, 1022 |
| Hpy166II | GTNNAC | 5 cut(s) | 210, 616, 697, 775, 938 |
| Hpy188I | TCNGA | 7 cut(s) | 276, 300, 438, 505, 675, 1012, 1188 |
| Hpy188III | TCNNGA | 4 cut(s) | 425, 722, 729, 913 |
| Hpy8I | GTNNAC | 5 cut(s) | 210, 616, 697, 775, 938 |
| Hpy99I | CGWCG | 2 cut(s) | 224, 1141 |
| HpyAV | CCTTC | 6 cut(s) | 174, 372, 393, 500, 847, 1055 |
| HpyCH4III | ACNGT | 6 cut(s) | 735, 772, 956, 989, 1000, 1034 |
| HpyCH4IV | ACGT | 1 cut(s) | 1118 |
| HpyCH4V | TGCA | 6 cut(s) | 151, 563, 616, 687, 705, 894 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 180, 361, 391 |
| HpyF3I | CTNAG | 4 cut(s) | 157, 194, 233, 553 |
| HpySE526I | ACGT | 1 cut(s) | 1118 |
| Hsp92II | CATG | 7 cut(s) | 173, 328, 709, 794, 944, 1057, 1224 |
| HspAI | GCGC | 4 cut(s) | 362, 973, 1166, 1203 |
| KflI | GGGWCCC | 1 cut(s) | 225 |
| Ksp22I | TGATCA | 1 cut(s) | 427 |
| KspAI | GTTAAC | 1 cut(s) | 697 |
| KspI | CCGCGG | 1 cut(s) | 1149 |
| Kzo9I | GATC | 1 cut(s) | 427 |
| LmnI | GCTCC | 1 cut(s) | 166 |
| Lsp1109I | GCAGC | 5 cut(s) | 161, 367, 514, 529, 1150 |
| LweI | GCATC | 2 cut(s) | 622, 1113 |
| MaeI | CTAG | 4 cut(s) | 99, 498, 848, 906 |
| MaeII | ACGT | 1 cut(s) | 1118 |
| MaeIII | GTNAC | 6 cut(s) | 116, 586, 735, 766, 826, 856 |
| MalI | GATC | 1 cut(s) | 429 |
| MboI | GATC | 1 cut(s) | 427 |
| MboII | GAAGA | 6 cut(s) | 77, 208, 289, 387, 688, 875 |
| MfeI | CAATTG | 1 cut(s) | 1129 |
| MhlI | GDGCHC | 2 cut(s) | 618, 987 |
| MluCI | AATT | 9 cut(s) | 330, 474, 491, 600, 717, 918, 930, 1129, 1209 |
| MlyI | GAGTC | 1 cut(s) | 343 |
| MmeI | TCCRAC | 2 cut(s) | 294, 865 |
| MseI | TTAA | 4 cut(s) | 333, 696, 1019, 1225 |
| MslI | CAYNNNNRTG | 3 cut(s) | 611, 764, 1035 |
| MspA1I | CMGCKG | 1 cut(s) | 1148 |
| MspI | CCGG | 4 cut(s) | 141, 1124, 1160, 1200 |
| MspR9I | CCNGG | 1 cut(s) | 1160 |
| MunI | CAATTG | 1 cut(s) | 1129 |
| MvnI | CGCG | 2 cut(s) | 549, 1148 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 180, 361, 391 |
| NciI | CCSGG | 1 cut(s) | 1160 |
| NcoI | CCATGG | 1 cut(s) | 1053 |
| NdeII | GATC | 1 cut(s) | 427 |
| NheI | GCTAGC | 1 cut(s) | 905 |
| NlaIII | CATG | 7 cut(s) | 173, 328, 709, 794, 944, 1057, 1224 |
| NlaIV | GGNNCC | 4 cut(s) | 226, 227, 640, 1051 |
| NmeAIII | GCCGAG | 1 cut(s) | 331 |
| NmuCI | GTSAC | 2 cut(s) | 766, 826 |
| NsbI | TGCGCA | 1 cut(s) | 1167 |
| NspI | RCATGY | 3 cut(s) | 709, 944, 1224 |
| PceI | AGGCCT | 1 cut(s) | 442 |
| PciI | ACATGT | 1 cut(s) | 1220 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 960 |
| PfeI | GAWTC | 4 cut(s) | 263, 557, 725, 833 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 371 |
| PkrI | GCNGC | 6 cut(s) | 176, 357, 384, 529, 544, 1165 |
| PleI | GAGTC | 1 cut(s) | 343 |
| PpsI | GAGTC | 1 cut(s) | 343 |
| PpuMI | RGGWCCY | 1 cut(s) | 225 |
| PscI | ACATGT | 1 cut(s) | 1220 |
| PshBI | ATTAAT | 1 cut(s) | 1019 |
| Psp5II | RGGWCCY | 1 cut(s) | 225 |
| PspEI | GGTNACC | 1 cut(s) | 856 |
| PspN4I | GGNNCC | 4 cut(s) | 226, 227, 640, 1051 |
| PspPI | GGNCC | 2 cut(s) | 10, 225 |
| PspPPI | RGGWCCY | 1 cut(s) | 225 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1005, 1037 |
| PstI | CTGCAG | 1 cut(s) | 565 |
| RseI | CAYNNNNRTG | 3 cut(s) | 611, 764, 1035 |
| SacII | CCGCGG | 1 cut(s) | 1149 |
| SaqAI | TTAA | 4 cut(s) | 333, 696, 1019, 1225 |
| SatI | GCNGC | 6 cut(s) | 175, 356, 383, 528, 543, 1164 |
| Sau3AI | GATC | 1 cut(s) | 427 |
| Sau96I | GGNCC | 2 cut(s) | 10, 225 |
| SchI | GAGTC | 1 cut(s) | 343 |
| ScrFI | CCNGG | 1 cut(s) | 1160 |
| SduI | GDGCHC | 2 cut(s) | 618, 987 |
| SfaNI | GCATC | 2 cut(s) | 622, 1113 |
| SfcI | CTRYAG | 1 cut(s) | 561 |
| Sfr303I | CCGCGG | 1 cut(s) | 1149 |
| SgrBI | CCGCGG | 1 cut(s) | 1149 |
| SinI | GGWCC | 1 cut(s) | 225 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 611, 764, 1035 |
| SpeI | ACTAGT | 1 cut(s) | 847 |
| Sse9I | AATT | 9 cut(s) | 330, 474, 491, 600, 717, 918, 930, 1129, 1209 |
| SseBI | AGGCCT | 1 cut(s) | 442 |
| SsiI | CCGC | 4 cut(s) | 383, 887, 1146, 1148 |
| SspMI | CTAG | 4 cut(s) | 99, 498, 848, 906 |
| StuI | AGGCCT | 1 cut(s) | 442 |
| StyD4I | CCNGG | 1 cut(s) | 1158 |
| StyI | CCWWGG | 2 cut(s) | 178, 1053 |
| TaaI | ACNGT | 6 cut(s) | 735, 772, 956, 989, 1000, 1034 |
| TaiI | ACGT | 1 cut(s) | 1121 |
| TaqI | TCGA | 3 cut(s) | 6, 730, 1107 |
| TasI | AATT | 9 cut(s) | 330, 474, 491, 600, 717, 918, 930, 1129, 1209 |
| TauI | GCSGC | 1 cut(s) | 385 |
| TfiI | GAWTC | 4 cut(s) | 263, 557, 725, 833 |
| Tru1I | TTAA | 4 cut(s) | 333, 696, 1019, 1225 |
| Tru9I | TTAA | 4 cut(s) | 333, 696, 1019, 1225 |
| TscAI | CASTG | 2 cut(s) | 618, 835 |
| TseFI | GTSAC | 2 cut(s) | 766, 826 |
| TseI | GCWGC | 5 cut(s) | 174, 355, 527, 542, 1163 |
| Tsp45I | GTSAC | 2 cut(s) | 766, 826 |
| TspDTI | ATGAA | 3 cut(s) | 498, 612, 995 |
| TspGWI | ACGGA | 1 cut(s) | 729 |
| TspRI | CASTG | 2 cut(s) | 618, 835 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 371 |
| VneI | GTGCAC | 1 cut(s) | 614 |
| VpaK11BI | GGWCC | 1 cut(s) | 225 |
| VspI | ATTAAT | 1 cut(s) | 1019 |
| XapI | RAATTY | 2 cut(s) | 717, 1209 |
| XceI | RCATGY | 3 cut(s) | 709, 944, 1224 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1093 |
| XmiI | GTMKAC | 1 cut(s) | 774 |
| XspI | CTAG | 4 cut(s) | 99, 498, 848, 906 |