RchiOBHm_Chr4g0435761

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009013
Physical Location & Seq
Reverse (-)
59199387 .. 59204024
4638 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40416

Sequence Viewer

Length: 1089 bp
ATGGCCGCCGTCGCCGGTCTGCACCTCTCAGCTCCGGTCCACGGCCAGGTCGTACTAAGCTCAGCCAAGTCAACTCTCTTCATGGCGACCCCGCTCACATTTCCACTCTCCACTCCCTCCGGAAGGGCGTCGTTTTCTCGGAAACAACTCGTCGTTTTTGCTCGGAGGCTTTCAGGCTTGGAAGAGGCCATGAGAATCAAAAGATTACGCGAGAGTCAAGTTACGACAAAGGTTAGAAGAACGGTGCCATTGAGGCGTGGAAGAGTGTCACCGACACTTCCTGTTCCAGATGACATTCCGAGGCCTCCTTATGCAGCTACATCTGTACTGCCAGAAATTGCCAGTGACTATCAATTTCCTGGTTCTGAAGGCATTGCTGCCATGAGAGCTGCATGTGAACTTGCTGCTCGTGTCTTAGACAATGCAGGGAAGTTAGTTAGGCCTTCAGTAACCACTAATGAAATTGACAAAGCAGTGCATCAGATGATTATTGATGCTGGTGCTTATCCCTCACCTCTTGGATATGGGGGATTTCCAAAGAGTGTGTGCACATCTGTTAACGAGTGCATGTGCCATGGAATACCTGATTCTCGTCAATTACAGGACGGAGACATCATAAACATAGATGTTACTGTCTACCTAAATGGATATCATGGGGACACATCAAAGACATTTCTTTGTGGGGAGGTCAGTGATAGTATGCAACGCCTAGTGAAGGTAACGGAAGAGTGCTTGGAAAGAGGCATAGCAGTTTGCAAGGATGGTGCTAGCTTCAAGAAAATTGGAAAGAGAATTAGTGAACATGCTGAAAAATTTGGTTATGGCGTGGTAGAACGTTTTGTTGGGCATGGTGTGGGTACTGTATTTCATTCTGAACCATTGATATTACATCACCGCAATGACCAGTCTGGTAGCATGGTTGAAGGTCAAACATTTACTATTGAACCCATCCTTACTATGGGAAGCATTGAGTGCGTAACATGGCCAGACAATTGGACAACTCTAACCGCCGATGGTAGCCCTGCCGCGCAGTTTGAGCATACCATTCTGATTACTAGAACTGGTGCTGAAATTTTAACCACATGTTGA

Protein Analysis

362

Amino Acids

39.2

Weight (kDa)

7.15

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M24 PF00557 127 - 352 4.7e-53 Metallopeptidase family M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 244
AccBSI CCGCTC 1 cut(s) 94
AccI GTMKAC 1 cut(s) 636
AccII CGCG 2 cut(s) 210, 1028
AccIII TCCGGA 1 cut(s) 119
AciI CCGC 5 cut(s) 6, 92, 895, 1008, 1026
AclI AACGTT 1 cut(s) 835
AcoI YGGCCR 3 cut(s) 3, 43, 983
AcsI RAATTY 2 cut(s) 812, 1071
AcuI CTGAAG 2 cut(s) 387, 429
AcyI GRCGYC 1 cut(s) 128
AfaI GTAC 3 cut(s) 54, 327, 859
AfiI CCNNNNNNNGG 5 cut(s) 41, 46, 123, 715, 958
AflIII ACRYGT 1 cut(s) 1082
AgsI TTSAA 3 cut(s) 775, 923, 944
AjnI CCWGG 2 cut(s) 45, 358
AjuI GAANNNNNNNTTGG 2 cut(s) 825, 857
AluBI AGCT 5 cut(s) 32, 60, 317, 389, 771
AluI AGCT 5 cut(s) 32, 60, 317, 389, 771
Alw21I GWGCWC 1 cut(s) 551
Alw26I GTCTC 1 cut(s) 603
Alw44I GTGCAC 1 cut(s) 547
Aor13HI TCCGGA 1 cut(s) 119
AoxI GGCC 6 cut(s) 3, 43, 186, 302, 440, 983
ApaLI GTGCAC 1 cut(s) 547
ApeKI GCWGC 4 cut(s) 314, 377, 389, 404
ApoI RAATTY 2 cut(s) 812, 1071
AspLEI GCGC 1 cut(s) 1030
AspS9I GGNCC 1 cut(s) 37
AsuHPI GGTGA 3 cut(s) 261, 504, 884
AsuNHI GCTAGC 1 cut(s) 767
AvaII GGWCC 1 cut(s) 37
BaeGI GKGCMC 1 cut(s) 551
BalI TGGCCA 1 cut(s) 985
BanI GGYRCC 1 cut(s) 244
BauI CACGAG 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 551
BbvI GCAGC 4 cut(s) 326, 364, 376, 391
BccI CCATC 3 cut(s) 755, 956, 1007
BceAI ACGGC 1 cut(s) 58
BciT130I CCWGG 2 cut(s) 47, 360
BcoDI GTCTC 1 cut(s) 603
BfaI CTAG 3 cut(s) 710, 768, 1056
BglI GCCNNNNNGGC 1 cut(s) 253
BisI GCNGC 6 cut(s) 6, 315, 378, 390, 405, 1026
BlpI GCTNAGC 1 cut(s) 61
BlsI GCNGC 6 cut(s) 7, 316, 379, 391, 406, 1027
Bme1390I CCNGG 2 cut(s) 47, 360
Bme18I GGWCC 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 1 cut(s) 246
BmrFI CCNGG 2 cut(s) 47, 360
BmsI GCATC 2 cut(s) 484, 487
BmtI GCTAGC 1 cut(s) 771
Bpu1102I GCTNAGC 1 cut(s) 61
BsaHI GRCGYC 1 cut(s) 128
BsaJI CCNNGG 3 cut(s) 40, 299, 574
BsaWI WCCGGW 2 cut(s) 34, 119
Bsc4I CCNNNNNNNGG 5 cut(s) 41, 46, 123, 715, 958
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 3 cut(s) 342, 904, 1066
Bse3DI GCAATG 2 cut(s) 372, 904
BseAI TCCGGA 1 cut(s) 119
BseBI CCWGG 2 cut(s) 47, 360
BseDI CCNNGG 3 cut(s) 40, 299, 574
BseGI GGATG 2 cut(s) 766, 948
BseLI CCNNNNNNNGG 5 cut(s) 41, 46, 123, 715, 958
BseMI GCAATG 2 cut(s) 372, 904
BseMII CTCAG 2 cut(s) 42, 75
BseNI ACTGG 3 cut(s) 342, 904, 1066
BseSI GKGCMC 1 cut(s) 551
BseXI GCAGC 4 cut(s) 326, 364, 376, 391
BsgI GTGCAG 1 cut(s) 5
Bsh1236I CGCG 2 cut(s) 210, 1028
BshFI GGCC 6 cut(s) 5, 45, 188, 304, 442, 985
BshNI GGYRCC 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 551
BsiSI CCGG 3 cut(s) 15, 35, 120
BslFI GGGAC 1 cut(s) 671
BslI CCNNNNNNNGG 5 cut(s) 41, 46, 123, 715, 958
BsmAI GTCTC 1 cut(s) 603
BsmFI GGGAC 1 cut(s) 671
BsnI GGCC 6 cut(s) 5, 45, 188, 304, 442, 985
Bsp1286I GDGCHC 1 cut(s) 551
Bsp13I TCCGGA 1 cut(s) 119
Bsp1720I GCTNAGC 1 cut(s) 61
Bsp19I CCATGG 1 cut(s) 574
BspACI CCGC 5 cut(s) 6, 92, 895, 1008, 1026
BspANI GGCC 6 cut(s) 5, 45, 188, 304, 442, 985
BspCNI CTCAG 2 cut(s) 41, 74
BspEI TCCGGA 1 cut(s) 119
BspFNI CGCG 2 cut(s) 210, 1028
BspLI GGNNCC 1 cut(s) 246
BspOI GCTAGC 1 cut(s) 771
BspT107I GGYRCC 1 cut(s) 244
BsrBI CCGCTC 1 cut(s) 94
BsrDI GCAATG 2 cut(s) 372, 904
BsrFI RCCGGY 1 cut(s) 14
BsrI ACTGG 3 cut(s) 342, 904, 1066
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 3 cut(s) 40, 299, 574
BssNI GRCGYC 1 cut(s) 128
BssSI CACGAG 1 cut(s) 408
BssT1I CCWWGG 1 cut(s) 574
Bst2BI CACGAG 1 cut(s) 408
Bst2UI CCWGG 2 cut(s) 47, 360
Bst4CI ACNGT 3 cut(s) 244, 634, 862
Bst6I CTCTTC 4 cut(s) 83, 177, 256, 720
BstACI GRCGYC 1 cut(s) 128
BstAPI GCANNNNNTGC 1 cut(s) 972
BstC8I GCNNGC 1 cut(s) 769
BstDEI CTNAG 4 cut(s) 28, 56, 61, 415
BstDSI CCRYGG 2 cut(s) 40, 574
BstENI CCTNNNNNAGG 2 cut(s) 121, 713
BstF5I GGATG 2 cut(s) 766, 948
BstFNI CGCG 2 cut(s) 210, 1028
BstHHI GCGC 1 cut(s) 1030
BstMAI GTCTC 1 cut(s) 603
BstMWI GCNNNNNNNGC 5 cut(s) 11, 253, 386, 972, 1036
BstNI CCWGG 2 cut(s) 47, 360
BstNSI RCATGY 4 cut(s) 396, 571, 806, 1086
BstSCI CCNGG 2 cut(s) 45, 358
BstSLI GKGCMC 1 cut(s) 551
BstUI CGCG 2 cut(s) 210, 1028
BstV1I GCAGC 4 cut(s) 326, 364, 376, 391
BstXI CCANNNNNNTGG 1 cut(s) 993
BsuRI GGCC 6 cut(s) 5, 45, 188, 304, 442, 985
BtgI CCRYGG 2 cut(s) 40, 574
BtsCI GGATG 2 cut(s) 766, 948
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 3 cut(s) 349, 480, 697
Cac8I GCNNGC 1 cut(s) 769
CfoI GCGC 1 cut(s) 1030
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 1 cut(s) 37
CseI GACGC 1 cut(s) 117
Csp6I GTAC 3 cut(s) 53, 326, 858
CviQI GTAC 3 cut(s) 53, 326, 858
DdeI CTNAG 4 cut(s) 28, 56, 61, 415
EaeI YGGCCR 3 cut(s) 3, 43, 983
Eam1104I CTCTTC 4 cut(s) 83, 177, 256, 720
EarI CTCTTC 4 cut(s) 83, 177, 256, 720
Eco130I CCWWGG 1 cut(s) 574
Eco147I AGGCCT 2 cut(s) 304, 442
Eco32I GATATC 1 cut(s) 650
Eco47I GGWCC 1 cut(s) 37
Eco57I CTGAAG 2 cut(s) 387, 429
EcoNI CCTNNNNNAGG 2 cut(s) 121, 713
EcoRII CCWGG 2 cut(s) 45, 358
EcoRV GATATC 1 cut(s) 650
EcoT14I CCWWGG 1 cut(s) 574
ErhI CCWWGG 1 cut(s) 574
FaqI GGGAC 1 cut(s) 671
FauI CCCGC 1 cut(s) 99
FblI GTMKAC 1 cut(s) 636
Fnu4HI GCNGC 6 cut(s) 6, 315, 378, 390, 405, 1026
FokI GGATG 2 cut(s) 773, 935
Fsp4HI GCNGC 6 cut(s) 6, 315, 378, 390, 405, 1026
FspBI CTAG 3 cut(s) 710, 768, 1056
GlaI GCGC 1 cut(s) 1029
GluI GCNGC 6 cut(s) 6, 315, 378, 390, 405, 1026
HaeIII GGCC 6 cut(s) 5, 45, 188, 304, 442, 985
HapII CCGG 3 cut(s) 15, 35, 120
HgaI GACGC 1 cut(s) 117
HhaI GCGC 1 cut(s) 1030
Hin1I GRCGYC 1 cut(s) 128
Hin6I GCGC 1 cut(s) 1028
HinP1I GCGC 1 cut(s) 1028
HincII GTYRAC 2 cut(s) 72, 559
HindII GTYRAC 2 cut(s) 72, 559
HinfI GANTC 3 cut(s) 195, 214, 587
HpaI GTTAAC 1 cut(s) 559
HpaII CCGG 3 cut(s) 15, 35, 120
HphI GGTGA 3 cut(s) 261, 504, 884
Hpy166II GTNNAC 7 cut(s) 40, 72, 398, 549, 559, 637, 800
Hpy188I TCNGA 7 cut(s) 141, 165, 300, 367, 483, 874, 1050
Hpy188III TCNNGA 3 cut(s) 120, 287, 775
Hpy8I GTNNAC 7 cut(s) 40, 72, 398, 549, 559, 637, 800
Hpy99I CGWCG 3 cut(s) 14, 133, 155
HpyAV CCTTC 5 cut(s) 117, 362, 453, 709, 917
HpyCH4III ACNGT 3 cut(s) 244, 634, 862
HpyCH4IV ACGT 1 cut(s) 835
HpyCH4V TGCA 9 cut(s) 22, 314, 392, 425, 478, 549, 567, 703, 756
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 253, 386, 972, 1036
HpyF3I CTNAG 4 cut(s) 28, 56, 61, 415
HpySE526I ACGT 1 cut(s) 835
Hsp92I GRCGYC 1 cut(s) 128
HspAI GCGC 1 cut(s) 1028
Kpn2I TCCGGA 1 cut(s) 119
KspAI GTTAAC 1 cut(s) 559
LmnI GCTCC 1 cut(s) 37
Lsp1109I GCAGC 4 cut(s) 326, 364, 376, 391
LweI GCATC 2 cut(s) 484, 487
MaeI CTAG 3 cut(s) 710, 768, 1056
MaeII ACGT 1 cut(s) 835
MaeIII GTNAC 7 cut(s) 220, 267, 344, 448, 628, 718, 976
MbiI CCGCTC 1 cut(s) 94
MboII GAAGA 5 cut(s) 70, 194, 249, 273, 737
MfeI CAATTG 1 cut(s) 991
MhlI GDGCHC 1 cut(s) 551
MlsI TGGCCA 1 cut(s) 985
MluCI AATT 9 cut(s) 336, 353, 462, 596, 780, 792, 812, 991, 1071
MluNI TGGCCA 1 cut(s) 985
MlyI GAGTC 1 cut(s) 223
Mox20I TGGCCA 1 cut(s) 985
MroI TCCGGA 1 cut(s) 119
MscI TGGCCA 1 cut(s) 985
MseI TTAA 2 cut(s) 558, 1076
MslI CAYNNNNRTG 1 cut(s) 897
Msp20I TGGCCA 1 cut(s) 985
MspI CCGG 3 cut(s) 15, 35, 120
MspR9I CCNGG 2 cut(s) 47, 360
MunI CAATTG 1 cut(s) 991
MvaI CCWGG 2 cut(s) 47, 360
MvnI CGCG 2 cut(s) 210, 1028
MwoI GCNNNNNNNGC 5 cut(s) 11, 253, 386, 972, 1036
NcoI CCATGG 1 cut(s) 574
NheI GCTAGC 1 cut(s) 767
NlaIV GGNNCC 1 cut(s) 246
NmuCI GTSAC 2 cut(s) 267, 344
NspI RCATGY 4 cut(s) 396, 571, 806, 1086
PceI AGGCCT 2 cut(s) 304, 442
PciI ACATGT 1 cut(s) 1082
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PfeI GAWTC 2 cut(s) 195, 587
PkrI GCNGC 6 cut(s) 7, 316, 379, 391, 406, 1027
PleI GAGTC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 222
PscI ACATGT 1 cut(s) 1082
Psp1406I AACGTT 1 cut(s) 835
Psp6I CCWGG 2 cut(s) 45, 358
PspGI CCWGG 2 cut(s) 45, 358
PspN4I GGNNCC 1 cut(s) 246
PspPI GGNCC 1 cut(s) 37
RsaI GTAC 3 cut(s) 54, 327, 859
RsaNI GTAC 3 cut(s) 53, 326, 858
RseI CAYNNNNRTG 1 cut(s) 897
SaqAI TTAA 2 cut(s) 558, 1076
SatI GCNGC 6 cut(s) 6, 315, 378, 390, 405, 1026
Sau96I GGNCC 1 cut(s) 37
SchI GAGTC 1 cut(s) 223
ScrFI CCNGG 2 cut(s) 47, 360
SduI GDGCHC 1 cut(s) 551
SfaNI GCATC 2 cut(s) 484, 487
SinI GGWCC 1 cut(s) 37
SmiMI CAYNNNNRTG 1 cut(s) 897
Sse9I AATT 9 cut(s) 336, 353, 462, 596, 780, 792, 812, 991, 1071
SseBI AGGCCT 2 cut(s) 304, 442
SsiI CCGC 5 cut(s) 6, 92, 895, 1008, 1026
SspMI CTAG 3 cut(s) 710, 768, 1056
StuI AGGCCT 2 cut(s) 304, 442
StyD4I CCNGG 2 cut(s) 45, 358
StyI CCWWGG 1 cut(s) 574
TaaI ACNGT 3 cut(s) 244, 634, 862
TaiI ACGT 1 cut(s) 838
TasI AATT 9 cut(s) 336, 353, 462, 596, 780, 792, 812, 991, 1071
TatI WGTACW 1 cut(s) 325
TauI GCSGC 2 cut(s) 8, 1028
TfiI GAWTC 2 cut(s) 195, 587
Tru1I TTAA 2 cut(s) 558, 1076
Tru9I TTAA 2 cut(s) 558, 1076
TscAI CASTG 3 cut(s) 349, 480, 697
TseFI GTSAC 2 cut(s) 267, 344
TseI GCWGC 4 cut(s) 314, 377, 389, 404
Tsp45I GTSAC 2 cut(s) 267, 344
TspDTI ATGAA 3 cut(s) 70, 474, 857
TspGWI ACGGA 2 cut(s) 621, 737
TspRI CASTG 3 cut(s) 349, 480, 697
VneI GTGCAC 1 cut(s) 547
VpaK11BI GGWCC 1 cut(s) 37
XagI CCTNNNNNAGG 2 cut(s) 121, 713
XapI RAATTY 2 cut(s) 812, 1071
XceI RCATGY 4 cut(s) 396, 571, 806, 1086
XcmI CCANNNNNNNNNTGG 1 cut(s) 955
XmiI GTMKAC 1 cut(s) 636
XspI CTAG 3 cut(s) 710, 768, 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.