Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val)
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G13270 AT1G13270 |
| fragaria_vesca | FvH4_4g28020 |
| malus_domestica | MD13G1046000.v1.1 MD16G1047600.v1.1 |
| prunus_persica | Prupe.1G306000_v2.0.a1 Prupe.1G306000_v2.0.a1 Prupe.1G306000_v2.0.a1 Prupe.1G306000_v2.0.a1 |
| pyrus_communis | pycom13g04040 pycom16g04160 |
| rosa_chinensis | RchiOBHm_Chr4g0435761 |
| rosa_laevigata | RLG00000006561 |
| rosa_multiflora | Rmu_co8005306.1_g000001 Rmu_sc0000051.1_g000022 Rmu_sc0036818.1_g000001 |
| rosa_rugosa | Rorug04G0289400 |
| rosa_samantha | Rh4AG343700 Rh4BG352400 Rh4CG366900 Rh4DG346300 |
| rosa_wichuraiana | Rw4G030010 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 806 |
| AccI | GTMKAC | 1 cut(s) | 942 |
| AccII | CGCG | 1 cut(s) | 717 |
| AccIII | TCCGGA | 1 cut(s) | 421 |
| AciI | CCGC | 6 cut(s) | 286, 316, 551, 578, 731, 1314 |
| AclWI | GGATC | 1 cut(s) | 72 |
| AcoI | YGGCCR | 2 cut(s) | 317, 1289 |
| AcsI | RAATTY | 4 cut(s) | 97, 173, 885, 1377 |
| AcuI | CTGAAG | 1 cut(s) | 693 |
| AfaI | GTAC | 1 cut(s) | 170 |
| AfeI | AGCGCT | 1 cut(s) | 1142 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 312, 539, 620, 1264 |
| AflIII | ACRYGT | 1 cut(s) | 1388 |
| AgeI | ACCGGT | 1 cut(s) | 1367 |
| AgsI | TTSAA | 7 cut(s) | 103, 180, 343, 1081, 1229, 1250, 1343 |
| AhlI | ACTAGT | 1 cut(s) | 1015 |
| AjiI | CACGTC | 1 cut(s) | 329 |
| AjnI | CCWGG | 1 cut(s) | 664 |
| AluBI | AGCT | 7 cut(s) | 303, 379, 472, 526, 695, 706, 1077 |
| AluI | AGCT | 7 cut(s) | 303, 379, 472, 526, 695, 706, 1077 |
| Alw21I | GWGCWC | 1 cut(s) | 786 |
| Alw26I | GTCTC | 2 cut(s) | 909, 957 |
| Alw44I | GTGCAC | 1 cut(s) | 782 |
| AlwI | GGATC | 1 cut(s) | 72 |
| Ama87I | CYCGRG | 1 cut(s) | 1274 |
| Aor13HI | TCCGGA | 1 cut(s) | 421 |
| Aor51HI | AGCGCT | 1 cut(s) | 1142 |
| AoxI | GGCC | 9 cut(s) | 137, 317, 323, 367, 489, 548, 608, 746, 1289 |
| ApaI | GGGCCC | 1 cut(s) | 371 |
| ApaLI | GTGCAC | 1 cut(s) | 782 |
| ApeKI | GCWGC | 4 cut(s) | 523, 695, 710, 1331 |
| ApoI | RAATTY | 4 cut(s) | 97, 173, 885, 1377 |
| AsiGI | ACCGGT | 1 cut(s) | 1367 |
| AspLEI | GCGC | 2 cut(s) | 532, 1143 |
| AspS9I | GGNCC | 4 cut(s) | 138, 367, 368, 399 |
| AsuC2I | CCSGG | 2 cut(s) | 365, 1328 |
| AsuHPI | GGTGA | 4 cut(s) | 73, 567, 810, 1190 |
| AsuNHI | GCTAGC | 1 cut(s) | 1073 |
| AvaI | CYCGRG | 1 cut(s) | 1274 |
| AvaII | GGWCC | 1 cut(s) | 399 |
| BaeGI | GKGCMC | 2 cut(s) | 371, 786 |
| BanI | GGYRCC | 1 cut(s) | 806 |
| BanII | GRGCYC | 1 cut(s) | 371 |
| Bbv12I | GWGCWC | 1 cut(s) | 786 |
| BbvI | GCAGC | 4 cut(s) | 535, 682, 697, 1318 |
| BccI | CCATC | 1 cut(s) | 1262 |
| BceAI | ACGGC | 1 cut(s) | 733 |
| BciT130I | CCWGG | 1 cut(s) | 666 |
| BcnI | CCSGG | 2 cut(s) | 365, 1328 |
| BcoDI | GTCTC | 2 cut(s) | 909, 957 |
| BcuI | ACTAGT | 1 cut(s) | 1015 |
| BfaI | CTAG | 3 cut(s) | 1016, 1074, 1362 |
| BfmI | CTRYAG | 1 cut(s) | 243 |
| BfoI | RGCGCY | 1 cut(s) | 1144 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 559 |
| BisI | GCNGC | 6 cut(s) | 317, 524, 551, 696, 711, 1332 |
| BlsI | GCNGC | 6 cut(s) | 318, 525, 552, 697, 712, 1333 |
| Bme1390I | CCNGG | 3 cut(s) | 365, 666, 1328 |
| Bme18I | GGWCC | 1 cut(s) | 399 |
| BmeT110I | CYCGRG | 1 cut(s) | 1274 |
| BmgBI | CACGTC | 1 cut(s) | 329 |
| BmgT120I | GGNCC | 4 cut(s) | 138, 367, 368, 399 |
| BmiI | GGNNCC | 5 cut(s) | 368, 369, 370, 808, 1219 |
| BmrFI | CCNGG | 3 cut(s) | 365, 666, 1328 |
| BmsI | GCATC | 1 cut(s) | 790 |
| BmtI | GCTAGC | 1 cut(s) | 1077 |
| BpuMI | CCSGG | 2 cut(s) | 365, 1328 |
| BsaJI | CCNNGG | 5 cut(s) | 320, 364, 605, 880, 1221 |
| BsaWI | WCCGGW | 2 cut(s) | 421, 1367 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 312, 539, 620, 1264 |
| Bse118I | RCCGGY | 2 cut(s) | 282, 1367 |
| Bse1I | ACTGG | 2 cut(s) | 648, 1210 |
| Bse3DI | GCAATG | 1 cut(s) | 678 |
| BseAI | TCCGGA | 1 cut(s) | 421 |
| BseBI | CCWGG | 1 cut(s) | 666 |
| BseDI | CCNNGG | 5 cut(s) | 320, 364, 605, 880, 1221 |
| BseGI | GGATG | 2 cut(s) | 997, 1254 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 312, 539, 620, 1264 |
| BseMI | GCAATG | 1 cut(s) | 678 |
| BseMII | CTCAG | 3 cut(s) | 313, 389, 1349 |
| BseNI | ACTGG | 2 cut(s) | 648, 1210 |
| BseRI | GAGGAG | 1 cut(s) | 500 |
| BseSI | GKGCMC | 2 cut(s) | 371, 786 |
| BseXI | GCAGC | 4 cut(s) | 535, 682, 697, 1318 |
| Bsh1236I | CGCG | 1 cut(s) | 717 |
| BshFI | GGCC | 9 cut(s) | 139, 319, 325, 369, 491, 550, 610, 748, 1291 |
| BshNI | GGYRCC | 1 cut(s) | 806 |
| BshTI | ACCGGT | 1 cut(s) | 1367 |
| BsiHKAI | GWGCWC | 1 cut(s) | 786 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1274 |
| BsiSI | CCGG | 6 cut(s) | 283, 364, 422, 1292, 1328, 1368 |
| BslFI | GGGAC | 1 cut(s) | 1177 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 312, 539, 620, 1264 |
| BsmAI | GTCTC | 2 cut(s) | 909, 957 |
| BsmFI | GGGAC | 1 cut(s) | 1177 |
| BsmI | GAATGC | 1 cut(s) | 875 |
| BsnI | GGCC | 9 cut(s) | 139, 319, 325, 369, 491, 550, 610, 748, 1291 |
| BsoBI | CYCGRG | 1 cut(s) | 1274 |
| Bsp120I | GGGCCC | 1 cut(s) | 367 |
| Bsp1286I | GDGCHC | 2 cut(s) | 371, 786 |
| Bsp13I | TCCGGA | 1 cut(s) | 421 |
| Bsp143I | GATC | 4 cut(s) | 63, 77, 537, 595 |
| Bsp19I | CCATGG | 2 cut(s) | 880, 1221 |
| BspACI | CCGC | 6 cut(s) | 286, 316, 551, 578, 731, 1314 |
| BspANI | GGCC | 9 cut(s) | 139, 319, 325, 369, 491, 550, 610, 748, 1291 |
| BspCNI | CTCAG | 3 cut(s) | 312, 388, 1348 |
| BspEI | TCCGGA | 1 cut(s) | 421 |
| BspFNI | CGCG | 1 cut(s) | 717 |
| BspLI | GGNNCC | 5 cut(s) | 368, 369, 370, 808, 1219 |
| BspMAI | CTGCAG | 1 cut(s) | 247 |
| BspOI | GCTAGC | 1 cut(s) | 1077 |
| BspPI | GGATC | 1 cut(s) | 72 |
| BspT107I | GGYRCC | 1 cut(s) | 806 |
| BsrDI | GCAATG | 1 cut(s) | 678 |
| BsrFI | RCCGGY | 2 cut(s) | 282, 1367 |
| BsrI | ACTGG | 2 cut(s) | 648, 1210 |
| BssAI | RCCGGY | 2 cut(s) | 282, 1367 |
| BssECI | CCNNGG | 5 cut(s) | 320, 364, 605, 880, 1221 |
| BssMI | GATC | 4 cut(s) | 63, 77, 537, 595 |
| BssT1I | CCWWGG | 4 cut(s) | 320, 605, 880, 1221 |
| Bst2UI | CCWGG | 1 cut(s) | 666 |
| Bst4CI | ACNGT | 6 cut(s) | 903, 940, 1070, 1168, 1202, 1322 |
| Bst6I | CTCTTC | 3 cut(s) | 237, 363, 1026 |
| BstC8I | GCNNGC | 8 cut(s) | 126, 284, 528, 693, 708, 715, 1075, 1110 |
| BstDEI | CTNAG | 5 cut(s) | 299, 336, 375, 721, 1335 |
| BstDSI | CCRYGG | 2 cut(s) | 880, 1221 |
| BstEII | GGTNACC | 1 cut(s) | 1024 |
| BstF5I | GGATG | 2 cut(s) | 997, 1254 |
| BstFNI | CGCG | 1 cut(s) | 717 |
| BstH2I | RGCGCY | 1 cut(s) | 1144 |
| BstHHI | GCGC | 2 cut(s) | 532, 1143 |
| BstKTI | GATC | 4 cut(s) | 66, 80, 540, 598 |
| BstMAI | GTCTC | 2 cut(s) | 909, 957 |
| BstMBI | GATC | 4 cut(s) | 63, 77, 537, 595 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 322, 529, 559, 1278 |
| BstNI | CCWGG | 1 cut(s) | 666 |
| BstNSI | RCATGY | 3 cut(s) | 877, 1112, 1392 |
| BstPI | GGTNACC | 1 cut(s) | 1024 |
| BstSCI | CCNGG | 3 cut(s) | 363, 664, 1326 |
| BstSFI | CTRYAG | 1 cut(s) | 243 |
| BstSLI | GKGCMC | 2 cut(s) | 371, 786 |
| BstUI | CGCG | 1 cut(s) | 717 |
| BstV1I | GCAGC | 4 cut(s) | 535, 682, 697, 1318 |
| BstX2I | RGATCY | 1 cut(s) | 77 |
| BstYI | RGATCY | 1 cut(s) | 77 |
| BsuRI | GGCC | 9 cut(s) | 139, 319, 325, 369, 491, 550, 610, 748, 1291 |
| BtgI | CCRYGG | 2 cut(s) | 880, 1221 |
| BtrI | CACGTC | 1 cut(s) | 329 |
| BtsCI | GGATG | 2 cut(s) | 997, 1254 |
| BtsI | GCAGTG | 1 cut(s) | 786 |
| BtsIMutI | CAGTG | 3 cut(s) | 655, 786, 1003 |
| Cac8I | GCNNGC | 8 cut(s) | 126, 284, 528, 693, 708, 715, 1075, 1110 |
| CfoI | GCGC | 2 cut(s) | 532, 1143 |
| Cfr10I | RCCGGY | 2 cut(s) | 282, 1367 |
| Cfr13I | GGNCC | 4 cut(s) | 138, 367, 368, 399 |
| CseI | GACGC | 1 cut(s) | 706 |
| Csp6I | GTAC | 1 cut(s) | 169 |
| CspAI | ACCGGT | 1 cut(s) | 1367 |
| CviQI | GTAC | 1 cut(s) | 169 |
| DdeI | CTNAG | 5 cut(s) | 299, 336, 375, 721, 1335 |
| DpnI | GATC | 4 cut(s) | 65, 79, 539, 597 |
| DpnII | GATC | 4 cut(s) | 63, 77, 537, 595 |
| EaeI | YGGCCR | 2 cut(s) | 317, 1289 |
| Eam1104I | CTCTTC | 3 cut(s) | 237, 363, 1026 |
| EarI | CTCTTC | 3 cut(s) | 237, 363, 1026 |
| Eco130I | CCWWGG | 4 cut(s) | 320, 605, 880, 1221 |
| Eco147I | AGGCCT | 1 cut(s) | 610 |
| Eco24I | GRGCYC | 1 cut(s) | 371 |
| Eco32I | GATATC | 1 cut(s) | 956 |
| Eco47I | GGWCC | 1 cut(s) | 399 |
| Eco47III | AGCGCT | 1 cut(s) | 1142 |
| Eco57I | CTGAAG | 1 cut(s) | 693 |
| Eco88I | CYCGRG | 1 cut(s) | 1274 |
| Eco91I | GGTNACC | 1 cut(s) | 1024 |
| EcoO109I | RGGNCCY | 2 cut(s) | 367, 368 |
| EcoO65I | GGTNACC | 1 cut(s) | 1024 |
| EcoRI | GAATTC | 1 cut(s) | 885 |
| EcoRII | CCWGG | 1 cut(s) | 664 |
| EcoRV | GATATC | 1 cut(s) | 956 |
| EcoT14I | CCWWGG | 4 cut(s) | 320, 605, 880, 1221 |
| EcoT22I | ATGCAT | 1 cut(s) | 875 |
| EcoT38I | GRGCYC | 1 cut(s) | 371 |
| ErhI | CCWWGG | 4 cut(s) | 320, 605, 880, 1221 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 598, 630 |
| FaqI | GGGAC | 1 cut(s) | 1177 |
| FauI | CCCGC | 1 cut(s) | 724 |
| FblI | GTMKAC | 1 cut(s) | 942 |
| Fnu4HI | GCNGC | 6 cut(s) | 317, 524, 551, 696, 711, 1332 |
| FokI | GGATG | 2 cut(s) | 1004, 1241 |
| FriOI | GRGCYC | 1 cut(s) | 371 |
| Fsp4HI | GCNGC | 6 cut(s) | 317, 524, 551, 696, 711, 1332 |
| FspBI | CTAG | 3 cut(s) | 1016, 1074, 1362 |
| GlaI | GCGC | 2 cut(s) | 531, 1142 |
| GluI | GCNGC | 6 cut(s) | 317, 524, 551, 696, 711, 1332 |
| HaeII | RGCGCY | 1 cut(s) | 1144 |
| HaeIII | GGCC | 9 cut(s) | 139, 319, 325, 369, 491, 550, 610, 748, 1291 |
| HapII | CCGG | 6 cut(s) | 283, 364, 422, 1292, 1328, 1368 |
| HgaI | GACGC | 1 cut(s) | 706 |
| HhaI | GCGC | 2 cut(s) | 532, 1143 |
| Hin6I | GCGC | 2 cut(s) | 530, 1141 |
| HinP1I | GCGC | 2 cut(s) | 530, 1141 |
| HincII | GTYRAC | 2 cut(s) | 352, 865 |
| HindII | GTYRAC | 2 cut(s) | 352, 865 |
| HinfI | GANTC | 7 cut(s) | 431, 498, 517, 725, 893, 1005, 1306 |
| HpaI | GTTAAC | 1 cut(s) | 865 |
| HpaII | CCGG | 6 cut(s) | 283, 364, 422, 1292, 1328, 1368 |
| HphI | GGTGA | 4 cut(s) | 73, 567, 810, 1190 |
| Hpy166II | GTNNAC | 6 cut(s) | 352, 653, 784, 865, 943, 1001 |
| Hpy188I | TCNGA | 7 cut(s) | 444, 468, 542, 673, 1180, 1311, 1356 |
| Hpy188III | TCNNGA | 5 cut(s) | 422, 593, 890, 897, 1081 |
| Hpy8I | GTNNAC | 6 cut(s) | 352, 653, 784, 865, 943, 1001 |
| Hpy99I | CGWCG | 1 cut(s) | 1322 |
| HpyAV | CCTTC | 5 cut(s) | 540, 561, 668, 1015, 1223 |
| HpyCH4III | ACNGT | 6 cut(s) | 903, 940, 1070, 1168, 1202, 1322 |
| HpyCH4IV | ACGT | 1 cut(s) | 328 |
| HpyCH4V | TGCA | 7 cut(s) | 69, 245, 293, 784, 855, 873, 1281 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 322, 529, 559, 1278 |
| HpyF3I | CTNAG | 5 cut(s) | 299, 336, 375, 721, 1335 |
| HpySE526I | ACGT | 1 cut(s) | 328 |
| HspAI | GCGC | 2 cut(s) | 530, 1141 |
| Kpn2I | TCCGGA | 1 cut(s) | 421 |
| KroI | GCCGGC | 1 cut(s) | 282 |
| KroNI | GCCGGC | 1 cut(s) | 284 |
| KspAI | GTTAAC | 1 cut(s) | 865 |
| Kzo9I | GATC | 4 cut(s) | 63, 77, 537, 595 |
| LmnI | GCTCC | 2 cut(s) | 308, 1138 |
| Lsp1109I | GCAGC | 4 cut(s) | 535, 682, 697, 1318 |
| LweI | GCATC | 1 cut(s) | 790 |
| MaeI | CTAG | 3 cut(s) | 1016, 1074, 1362 |
| MaeII | ACGT | 1 cut(s) | 328 |
| MaeIII | GTNAC | 5 cut(s) | 115, 256, 903, 934, 1024 |
| MalI | GATC | 4 cut(s) | 65, 79, 539, 597 |
| MboI | GATC | 4 cut(s) | 63, 77, 537, 595 |
| MboII | GAAGA | 4 cut(s) | 224, 350, 555, 1043 |
| MfeI | CAATTG | 1 cut(s) | 1297 |
| MflI | RGATCY | 1 cut(s) | 77 |
| MhlI | GDGCHC | 2 cut(s) | 371, 786 |
| MlyI | GAGTC | 3 cut(s) | 526, 734, 1300 |
| MmeI | TCCRAC | 2 cut(s) | 462, 1033 |
| Mph1103I | ATGCAT | 1 cut(s) | 875 |
| MreI | CGCCGGCG | 1 cut(s) | 282 |
| MroI | TCCGGA | 1 cut(s) | 421 |
| MroNI | GCCGGC | 1 cut(s) | 282 |
| MseI | TTAA | 2 cut(s) | 864, 1393 |
| MslI | CAYNNNNRTG | 2 cut(s) | 932, 1203 |
| MspI | CCGG | 6 cut(s) | 283, 364, 422, 1292, 1328, 1368 |
| MspR9I | CCNGG | 3 cut(s) | 365, 666, 1328 |
| MunI | CAATTG | 1 cut(s) | 1297 |
| Mva1269I | GAATGC | 1 cut(s) | 875 |
| MvaI | CCWGG | 1 cut(s) | 666 |
| MvnI | CGCG | 1 cut(s) | 717 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 322, 529, 559, 1278 |
| NaeI | GCCGGC | 1 cut(s) | 284 |
| NciI | CCSGG | 2 cut(s) | 365, 1328 |
| NcoI | CCATGG | 2 cut(s) | 880, 1221 |
| NdeII | GATC | 4 cut(s) | 63, 77, 537, 595 |
| NgoMIV | GCCGGC | 1 cut(s) | 282 |
| NheI | GCTAGC | 1 cut(s) | 1073 |
| NlaIV | GGNNCC | 5 cut(s) | 368, 369, 370, 808, 1219 |
| NmuCI | GTSAC | 1 cut(s) | 934 |
| NsiI | ATGCAT | 1 cut(s) | 875 |
| NspI | RCATGY | 3 cut(s) | 877, 1112, 1392 |
| PaeI | GCATGC | 1 cut(s) | 1112 |
| PaeR7I | CTCGAG | 1 cut(s) | 1274 |
| PceI | AGGCCT | 1 cut(s) | 610 |
| PciI | ACATGT | 1 cut(s) | 1388 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1009 |
| PctI | GAATGC | 1 cut(s) | 875 |
| PdiI | GCCGGC | 1 cut(s) | 284 |
| PfeI | GAWTC | 4 cut(s) | 431, 498, 893, 1005 |
| PinAI | ACCGGT | 1 cut(s) | 1367 |
| PkrI | GCNGC | 6 cut(s) | 318, 525, 552, 697, 712, 1333 |
| PleI | GAGTC | 3 cut(s) | 525, 733, 1300 |
| PpsI | GAGTC | 3 cut(s) | 525, 733, 1300 |
| PscI | ACATGT | 1 cut(s) | 1388 |
| Psp6I | CCWGG | 1 cut(s) | 664 |
| PspEI | GGTNACC | 1 cut(s) | 1024 |
| PspGI | CCWGG | 1 cut(s) | 664 |
| PspN4I | GGNNCC | 5 cut(s) | 368, 369, 370, 808, 1219 |
| PspOMI | GGGCCC | 1 cut(s) | 367 |
| PspPI | GGNCC | 4 cut(s) | 138, 367, 368, 399 |
| PspXI | VCTCGAGB | 1 cut(s) | 1274 |
| PstI | CTGCAG | 1 cut(s) | 247 |
| PsuI | RGATCY | 1 cut(s) | 77 |
| RsaI | GTAC | 1 cut(s) | 170 |
| RsaNI | GTAC | 1 cut(s) | 169 |
| RseI | CAYNNNNRTG | 2 cut(s) | 932, 1203 |
| SaqAI | TTAA | 2 cut(s) | 864, 1393 |
| SatI | GCNGC | 6 cut(s) | 317, 524, 551, 696, 711, 1332 |
| Sau3AI | GATC | 4 cut(s) | 63, 77, 537, 595 |
| Sau96I | GGNCC | 4 cut(s) | 138, 367, 368, 399 |
| SchI | GAGTC | 3 cut(s) | 526, 734, 1300 |
| ScrFI | CCNGG | 3 cut(s) | 365, 666, 1328 |
| SduI | GDGCHC | 2 cut(s) | 371, 786 |
| SfaNI | GCATC | 1 cut(s) | 790 |
| SfcI | CTRYAG | 1 cut(s) | 243 |
| Sfr274I | CTCGAG | 1 cut(s) | 1274 |
| SgrAI | CRCCGGYG | 1 cut(s) | 282 |
| SinI | GGWCC | 1 cut(s) | 399 |
| SlaI | CTCGAG | 1 cut(s) | 1274 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 932, 1203 |
| SmlI | CTYRAG | 1 cut(s) | 1274 |
| SmoI | CTYRAG | 1 cut(s) | 1274 |
| SpeI | ACTAGT | 1 cut(s) | 1015 |
| SphI | GCATGC | 1 cut(s) | 1112 |
| SseBI | AGGCCT | 1 cut(s) | 610 |
| SsiI | CCGC | 6 cut(s) | 286, 316, 551, 578, 731, 1314 |
| SspMI | CTAG | 3 cut(s) | 1016, 1074, 1362 |
| StuI | AGGCCT | 1 cut(s) | 610 |
| StyD4I | CCNGG | 3 cut(s) | 363, 664, 1326 |
| StyI | CCWWGG | 4 cut(s) | 320, 605, 880, 1221 |
| TaaI | ACNGT | 6 cut(s) | 903, 940, 1070, 1168, 1202, 1322 |
| TaiI | ACGT | 1 cut(s) | 331 |
| TaqI | TCGA | 3 cut(s) | 44, 898, 1275 |
| TauI | GCSGC | 2 cut(s) | 319, 553 |
| TfiI | GAWTC | 4 cut(s) | 431, 498, 893, 1005 |
| Tru1I | TTAA | 2 cut(s) | 864, 1393 |
| Tru9I | TTAA | 2 cut(s) | 864, 1393 |
| TscAI | CASTG | 3 cut(s) | 655, 786, 1003 |
| TseFI | GTSAC | 1 cut(s) | 934 |
| TseI | GCWGC | 4 cut(s) | 523, 695, 710, 1331 |
| Tsp45I | GTSAC | 1 cut(s) | 934 |
| TspDTI | ATGAA | 2 cut(s) | 615, 780 |
| TspRI | CASTG | 3 cut(s) | 655, 786, 1003 |
| VneI | GTGCAC | 1 cut(s) | 782 |
| VpaK11BI | GGWCC | 1 cut(s) | 399 |
| XapI | RAATTY | 4 cut(s) | 97, 173, 885, 1377 |
| XceI | RCATGY | 3 cut(s) | 877, 1112, 1392 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1261 |
| XhoI | CTCGAG | 1 cut(s) | 1274 |
| XmiI | GTMKAC | 1 cut(s) | 942 |
| XspI | CTAG | 3 cut(s) | 1016, 1074, 1362 |
| Zsp2I | ATGCAT | 1 cut(s) | 875 |