pycom13g04420

phosphate transporter PHO1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011751
Physical Location & Seq
Forward (+)
3005735 .. 3007408
1674 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 846 bp
ATGCCCAAGTTCAAGAAATTCCTGGCCTTCTTTTGTTGGTTTTTAGTACTACTTGTGTGCCCCTTCAACATCATTTACCGATCAAGCCGTTTCCGCCTTCTTCGTGTCCTAAGAAATATCATTCTGTCGCCTCTGTACAAGGTTGTCATGCTGGACTTCTTCATGGCAGATCAACTTTGTAGTCAGGTTCCGATGCTTAGGAACCTCGAGTATGTGGCGTGTTACTACATAACCGGGAGCTACAAAACTCAGGACTATGGCTACTGCATGAGGGCGGGGCATTACCGAGATCTTGCTTATGCCGTGTCCTTCCTACCTTATTACTGGAGAGCAATGCAGTGTGCTCGGCGGTGGTTTGATGAGGGACAGACAAGCCACCTTGTGAATCTAGGCAAATATGTGTCAGCAATGTTAGCAGCAGTAGCTAAAGTAGCTTATGAGAAAGAAGGGAGCATTGGGTGGCTCTGTCTTGTTGTGATCATATCGACTTTCGCAACAATTTACCAATTGTATTGGGACTTTGTAAAGGATTGGGGTTTGCTACAAATGAATTCCAAGAATCCTTTGCTTAGGAACGAATTAATGATTCGCCGAAAGTACATATACTACATCTCAATGGGATTGAACCTTATTCTCAGGCTAGCTTGGATGCAAACTGTTCTCCATTCAAGTTTTGGACATGTAGATTACAGGGTAACCGGGCTGTTTTTGGCAGCCCTTGAAGTCGTCAGAAGAGGGTTGTGGAACTTTTTCAGGTTAGAGAATGAACATCTGAACAATGCAGGCAAGTTTAGAGCAGTTAAGACAGTACCACTGCCTTTTCATGAAGTGGATGATCAGGACTGA

Protein Analysis

282

Amino Acids

33.24

Weight (kDa)

9.42

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXS PF03124 8 - 258 1.5e-81 EXS family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011751)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68740 AT1G68740
fragaria_vesca FvH4_4g28410
malus_domestica MD13G1049800.v1.1 MD16G1050800.v1.1
prunus_persica Prupe.1G301900_v2.0.a1
pyrus_communis pycom13g04410 pycom13g04420 pycom16g04450
rosa_chinensis RchiOBHm_Chr4g0436361
rosa_laevigata RLG00000006525
rosa_multiflora Rmu_sc0006449.1_g000007
rosa_roxburghii Rroxscaffold_5G00377530
rosa_rugosa Rorug04G0293300
rosa_samantha Rh4AG347500 Rh4BG356300 Rh4CG370900 Rh4DG350000
rosa_wichuraiana Rw4G030380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 94, 275, 349
AcsI RAATTY 2 cut(s) 17, 550
AdeI CACNNNGTG 1 cut(s) 382
AfaI GTAC 4 cut(s) 48, 137, 599, 810
AflIII ACRYGT 1 cut(s) 679
AgsI TTSAA 5 cut(s) 13, 67, 625, 669, 722
AjnI CCWGG 1 cut(s) 21
AjuI GAANNNNNNNTTGG 2 cut(s) 438, 470
AluBI AGCT 4 cut(s) 240, 425, 434, 644
AluI AGCT 4 cut(s) 240, 425, 434, 644
Alw21I GWGCWC 1 cut(s) 346
Ama87I CYCGRG 1 cut(s) 206
AoxI GGCC 1 cut(s) 24
ApeKI GCWGC 2 cut(s) 416, 713
ApoI RAATTY 2 cut(s) 17, 550
AseI ATTAAT 1 cut(s) 581
Asp700I GAANNNNTTC 1 cut(s) 749
AsuC2I CCSGG 2 cut(s) 235, 700
AsuNHI GCTAGC 1 cut(s) 640
AvaI CYCGRG 1 cut(s) 206
BaeGI GKGCMC 1 cut(s) 62
Bbv12I GWGCWC 1 cut(s) 346
BbvI GCAGC 2 cut(s) 428, 725
BceAI ACGGC 2 cut(s) 72, 287
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BciT130I CCWGG 1 cut(s) 23
BclI TGATCA 2 cut(s) 477, 835
BcnI CCSGG 2 cut(s) 235, 700
BfaI CTAG 2 cut(s) 389, 641
BglII AGATCT 1 cut(s) 289
BisI GCNGC 2 cut(s) 417, 714
BlsI GCNGC 2 cut(s) 418, 715
BmcAI AGTACT 1 cut(s) 48
Bme1390I CCNGG 3 cut(s) 23, 235, 700
BmeT110I CYCGRG 1 cut(s) 206
BmiI GGNNCC 2 cut(s) 189, 203
BmrFI CCNGG 3 cut(s) 23, 235, 700
BmsI GCATC 2 cut(s) 183, 639
BmtI GCTAGC 1 cut(s) 644
BpmI CTGGAG 1 cut(s) 346
Bpu10I CCTNAGC 2 cut(s) 197, 569
BpuMI CCSGG 2 cut(s) 235, 700
Bse1I ACTGG 1 cut(s) 329
Bse3DI GCAATG 2 cut(s) 339, 414
BseBI CCWGG 1 cut(s) 23
BseGI GGATG 2 cut(s) 654, 838
BseMI GCAATG 2 cut(s) 339, 414
BseMII CTCAG 2 cut(s) 263, 649
BseNI ACTGG 1 cut(s) 329
BseSI GKGCMC 1 cut(s) 62
BseXI GCAGC 2 cut(s) 428, 725
BshFI GGCC 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 346
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 2 cut(s) 234, 699
BslFI GGGAC 2 cut(s) 378, 530
BsmFI GGGAC 2 cut(s) 378, 530
BsnI GGCC 1 cut(s) 26
BsoBI CYCGRG 1 cut(s) 206
Bsp1286I GDGCHC 2 cut(s) 62, 346
Bsp1407I TGTACA 1 cut(s) 135
Bsp143I GATC 5 cut(s) 80, 169, 289, 477, 835
BspACI CCGC 3 cut(s) 94, 275, 349
BspANI GGCC 1 cut(s) 26
BspCNI CTCAG 2 cut(s) 262, 648
BspHI TCATGA 1 cut(s) 823
BspLI GGNNCC 2 cut(s) 189, 203
BspOI GCTAGC 1 cut(s) 644
BsrDI GCAATG 2 cut(s) 339, 414
BsrGI TGTACA 1 cut(s) 135
BsrI ACTGG 1 cut(s) 329
BssMI GATC 5 cut(s) 80, 169, 289, 477, 835
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 2 cut(s) 658, 808
Bst6I CTCTTC 1 cut(s) 727
BstAUI TGTACA 1 cut(s) 135
BstC8I GCNNGC 2 cut(s) 642, 784
BstDEI CTNAG 5 cut(s) 110, 197, 249, 569, 635
BstEII GGTNACC 1 cut(s) 694
BstF5I GGATG 2 cut(s) 654, 838
BstKTI GATC 5 cut(s) 83, 172, 292, 480, 838
BstMBI GATC 5 cut(s) 80, 169, 289, 477, 835
BstMWI GCNNNNNNNGC 4 cut(s) 93, 413, 422, 431
BstNI CCWGG 1 cut(s) 23
BstNSI RCATGY 1 cut(s) 683
BstPI GGTNACC 1 cut(s) 694
BstSCI CCNGG 3 cut(s) 21, 233, 698
BstSLI GKGCMC 1 cut(s) 62
BstV1I GCAGC 2 cut(s) 428, 725
BstX2I RGATCY 1 cut(s) 289
BstYI RGATCY 1 cut(s) 289
BsuRI GGCC 1 cut(s) 26
BtsCI GGATG 2 cut(s) 654, 838
BtsI GCAGTG 2 cut(s) 344, 812
BtsIMutI CAGTG 2 cut(s) 344, 812
Cac8I GCNNGC 2 cut(s) 642, 784
CciI TCATGA 1 cut(s) 823
Csp6I GTAC 4 cut(s) 47, 136, 598, 809
CviAII CATG 5 cut(s) 148, 163, 268, 680, 824
CviQI GTAC 4 cut(s) 47, 136, 598, 809
DdeI CTNAG 5 cut(s) 110, 197, 249, 569, 635
DpnI GATC 5 cut(s) 82, 171, 291, 479, 837
DpnII GATC 5 cut(s) 80, 169, 289, 477, 835
DraIII CACNNNGTG 1 cut(s) 382
Eam1104I CTCTTC 1 cut(s) 727
EarI CTCTTC 1 cut(s) 727
EciI GGCGGA 1 cut(s) 83
Eco88I CYCGRG 1 cut(s) 206
Eco91I GGTNACC 1 cut(s) 694
EcoO65I GGTNACC 1 cut(s) 694
EcoRI GAATTC 1 cut(s) 550
EcoRII CCWGG 1 cut(s) 21
FaeI CATG 5 cut(s) 151, 166, 271, 683, 827
FaqI GGGAC 2 cut(s) 378, 530
FatI CATG 5 cut(s) 147, 162, 267, 679, 823
FauI CCCGC 1 cut(s) 268
FbaI TGATCA 2 cut(s) 477, 835
Fnu4HI GCNGC 2 cut(s) 417, 714
FokI GGATG 1 cut(s) 661
Fsp4HI GCNGC 2 cut(s) 417, 714
FspBI CTAG 2 cut(s) 389, 641
GluI GCNGC 2 cut(s) 417, 714
GsuI CTGGAG 1 cut(s) 346
HaeIII GGCC 1 cut(s) 26
HapII CCGG 2 cut(s) 234, 699
Hin1II CATG 5 cut(s) 151, 166, 271, 683, 827
HinfI GANTC 3 cut(s) 385, 559, 586
HpaII CCGG 2 cut(s) 234, 699
Hpy188I TCNGA 3 cut(s) 192, 731, 774
Hpy188III TCNNGA 4 cut(s) 13, 251, 824, 839
HpyAV CCTTC 5 cut(s) 37, 73, 107, 319, 440
HpyCH4III ACNGT 2 cut(s) 658, 808
HpyCH4V TGCA 4 cut(s) 267, 337, 652, 782
HpyF10VI GCNNNNNNNGC 4 cut(s) 93, 413, 422, 431
HpyF3I CTNAG 5 cut(s) 110, 197, 249, 569, 635
Hsp92II CATG 5 cut(s) 151, 166, 271, 683, 827
Ksp22I TGATCA 2 cut(s) 477, 835
Kzo9I GATC 5 cut(s) 80, 169, 289, 477, 835
LmnI GCTCC 2 cut(s) 237, 450
Lsp1109I GCAGC 2 cut(s) 428, 725
LweI GCATC 2 cut(s) 183, 639
MaeI CTAG 2 cut(s) 389, 641
MaeIII GTNAC 2 cut(s) 221, 694
MalI GATC 5 cut(s) 82, 171, 291, 479, 837
MboI GATC 5 cut(s) 80, 169, 289, 477, 835
MboII GAAGA 3 cut(s) 92, 151, 744
MfeI CAATTG 1 cut(s) 506
MflI RGATCY 1 cut(s) 289
MhlI GDGCHC 2 cut(s) 62, 346
MluCI AATT 5 cut(s) 17, 498, 506, 550, 578
MnlI CCTC 5 cut(s) 141, 215, 264, 355, 728
MroXI GAANNNNTTC 1 cut(s) 749
MseI TTAA 2 cut(s) 581, 801
MslI CAYNNNNRTG 1 cut(s) 614
MspI CCGG 2 cut(s) 234, 699
MspR9I CCNGG 3 cut(s) 23, 235, 700
MunI CAATTG 1 cut(s) 506
MvaI CCWGG 1 cut(s) 23
MwoI GCNNNNNNNGC 4 cut(s) 93, 413, 422, 431
NciI CCSGG 2 cut(s) 235, 700
NdeII GATC 5 cut(s) 80, 169, 289, 477, 835
NheI GCTAGC 1 cut(s) 640
NlaIII CATG 5 cut(s) 151, 166, 271, 683, 827
NlaIV GGNNCC 2 cut(s) 189, 203
NmeAIII GCCGAG 1 cut(s) 325
NspI RCATGY 1 cut(s) 683
PaeR7I CTCGAG 1 cut(s) 206
PagI TCATGA 1 cut(s) 823
PciI ACATGT 1 cut(s) 679
PdmI GAANNNNTTC 1 cut(s) 749
PfeI GAWTC 3 cut(s) 385, 559, 586
PkrI GCNGC 2 cut(s) 418, 715
PscI ACATGT 1 cut(s) 679
PshBI ATTAAT 1 cut(s) 581
Psp6I CCWGG 1 cut(s) 21
PspEI GGTNACC 1 cut(s) 694
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 2 cut(s) 189, 203
PspXI VCTCGAGB 1 cut(s) 206
PsuI RGATCY 1 cut(s) 289
RsaI GTAC 4 cut(s) 48, 137, 599, 810
RsaNI GTAC 4 cut(s) 47, 136, 598, 809
RseI CAYNNNNRTG 1 cut(s) 614
SaqAI TTAA 2 cut(s) 581, 801
SatI GCNGC 2 cut(s) 417, 714
Sau3AI GATC 5 cut(s) 80, 169, 289, 477, 835
ScaI AGTACT 1 cut(s) 48
ScrFI CCNGG 3 cut(s) 23, 235, 700
SduI GDGCHC 2 cut(s) 62, 346
SfaNI GCATC 2 cut(s) 183, 639
Sfr274I CTCGAG 1 cut(s) 206
SlaI CTCGAG 1 cut(s) 206
SmiMI CAYNNNNRTG 1 cut(s) 614
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 5 cut(s) 17, 498, 506, 550, 578
SsiI CCGC 3 cut(s) 94, 275, 349
SspMI CTAG 2 cut(s) 389, 641
StyD4I CCNGG 3 cut(s) 21, 233, 698
TaaI ACNGT 2 cut(s) 658, 808
TaqI TCGA 2 cut(s) 207, 485
TasI AATT 5 cut(s) 17, 498, 506, 550, 578
TatI WGTACW 3 cut(s) 46, 135, 597
TfiI GAWTC 3 cut(s) 385, 559, 586
Tru1I TTAA 2 cut(s) 581, 801
Tru9I TTAA 2 cut(s) 581, 801
TscAI CASTG 2 cut(s) 344, 819
TseI GCWGC 2 cut(s) 416, 713
TspDTI ATGAA 5 cut(s) 151, 563, 780, 812, 840
TspRI CASTG 2 cut(s) 344, 819
VspI ATTAAT 1 cut(s) 581
XapI RAATTY 2 cut(s) 17, 550
XceI RCATGY 1 cut(s) 683
XcmI CCANNNNNNNNNTGG 1 cut(s) 671
XhoI CTCGAG 1 cut(s) 206
XmnI GAANNNNTTC 1 cut(s) 749
XspI CTAG 2 cut(s) 389, 641
ZrmI AGTACT 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.