pycom13g12560

YT521-B-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0006149
Physical Location & Seq
Forward (+)
8751998 .. 8753175
1178 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 804 bp
ATGGGTGGCCCGGTTGACTTTCACAAGGATATGGACTTTTGGCAGCAAGATAAATGGAGTGGGAGCTTCCCTGTTAAGTGGCACATTATTAAAGATGTTCCAAATACCAGTTTCAGGCACATTGTATTGGAGAACAATGAAAACAAGCCGGTGACTAATAGCAGGGATACGCAAGAGATTATGTATAAGAAAGGCTTGGAGATGCTGAAAATATTCAAGAATCACACATTGAAGACATCTTTACTTGATGACTTTATGTACTATGAGGACCGTCAGAAAATCATGCAGGAAGAGAGAGGCAGGTTACTTAGAAGCTTTGAAAGTCCATTTTTAGTACCTGGGCTGGGTCCTCCCCGCAAGCTGCACAACTCTGTGGGTGAGCTGCCTCGTATTAAGGACGAAAAAGTCACCAAACTTAATGATGATGTTAACAACGCGAAAAAGACTTTGGTCTCTACTTCTGAGCGGGTTTCCTCTAACTCAAGTGTTATCAACGCCAGTATCAAGAAAGAAGATGTTGAGCGGAAGGCAATTGAAGCAAAAGATGATGCCGTGTCTACCTTAAAAATTGATTCCCTCACTATTACTCCAAAGCAGGCAGGATCAAAATCTTCAGCTGCTGCTGTTACTAACACCAGAAAGGTTGATGTTGTCACTGTAGGCTCAATGCCCGTAAGAGTTAACGGGTATTCTGAATCCTTGGGTAATTTAACAGTTGGAACAATCCCTCTGGATCCTAGAGCGTTACAGCTAGAGAAGGGCGGGTCTATTATCAAAAATGCAGCTCAACCTCAAGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YTH

Protein Analysis

268

Amino Acids

29.91

Weight (kDa)

9.27

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YTH PF04146 1 - 73 3e-20 YT521-B-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 404
Acc36I ACCTGC 1 cut(s) 291
AccBSI CCGCTC 2 cut(s) 466, 523
AccI GTMKAC 1 cut(s) 557
AccII CGCG 1 cut(s) 437
AciI CCGC 4 cut(s) 355, 466, 523, 762
AclWI GGATC 3 cut(s) 610, 728, 741
AcuI CTGAAG 1 cut(s) 597
AfaI GTAC 2 cut(s) 260, 336
AfiI CCNNNNNNNGG 2 cut(s) 114, 344
AgsI TTSAA 4 cut(s) 217, 232, 320, 536
AjnI CCWGG 1 cut(s) 337
AjuI GAANNNNNNNTTGG 2 cut(s) 431, 463
AluBI AGCT 7 cut(s) 66, 315, 361, 382, 617, 751, 785
AluI AGCT 7 cut(s) 66, 315, 361, 382, 617, 751, 785
Alw26I GTCTC 1 cut(s) 457
AlwI GGATC 3 cut(s) 610, 728, 741
AlwNI CAGNNNCTG 1 cut(s) 620
AoxI GGCC 1 cut(s) 7
ApeKI GCWGC 6 cut(s) 43, 361, 382, 617, 620, 782
Asp700I GAANNNNTTC 1 cut(s) 212
AspS9I GGNCC 3 cut(s) 8, 268, 347
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 3 cut(s) 163, 389, 400
AvaII GGWCC 2 cut(s) 268, 347
BaeI ACNNNNGTAYC 2 cut(s) 484, 517
BamHI GGATCC 1 cut(s) 733
BbsI GAAGAC 1 cut(s) 239
BbvI GCAGC 6 cut(s) 55, 348, 369, 604, 607, 794
BceAI ACGGC 1 cut(s) 536
BciT130I CCWGG 1 cut(s) 339
BciVI GTATCC 1 cut(s) 160
BcnI CCSGG 1 cut(s) 11
BcoDI GTCTC 1 cut(s) 457
BfaI CTAG 2 cut(s) 738, 752
BfmI CTRYAG 1 cut(s) 657
BfuAI ACCTGC 1 cut(s) 291
BfuI GTATCC 1 cut(s) 160
BisI GCNGC 6 cut(s) 44, 362, 383, 618, 621, 783
BlsI GCNGC 6 cut(s) 45, 363, 384, 619, 622, 784
Bme1390I CCNGG 2 cut(s) 11, 339
Bme18I GGWCC 2 cut(s) 268, 347
BmgT120I GGNCC 3 cut(s) 8, 268, 347
BmiI GGNNCC 2 cut(s) 348, 735
BmrFI CCNGG 2 cut(s) 11, 339
BmsI GCATC 2 cut(s) 192, 538
BoxI GACNNNNGTC 1 cut(s) 449
BpiI GAAGAC 1 cut(s) 239
BpuEI CTTGAG 2 cut(s) 466, 777
BpuMI CCSGG 1 cut(s) 11
BsaBI GATNNNNATC 1 cut(s) 607
BsaI GGTCTC 1 cut(s) 457
BsaJI CCNNGG 2 cut(s) 338, 699
BsaXI ACNNNNNCTCC 2 cut(s) 571, 601
Bsc4I CCNNNNNNNGG 2 cut(s) 114, 344
Bse118I RCCGGY 1 cut(s) 148
Bse1I ACTGG 2 cut(s) 108, 498
Bse8I GATNNNNATC 1 cut(s) 607
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 2 cut(s) 338, 699
BseJI GATNNNNATC 1 cut(s) 607
BseLI CCNNNNNNNGG 2 cut(s) 114, 344
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 2 cut(s) 108, 498
BseXI GCAGC 6 cut(s) 55, 348, 369, 604, 607, 794
BseYI CCCAGC 1 cut(s) 343
BsgI GTGCAG 1 cut(s) 347
Bsh1236I CGCG 1 cut(s) 437
BshFI GGCC 1 cut(s) 9
BsiSI CCGG 2 cut(s) 11, 149
BslI CCNNNNNNNGG 2 cut(s) 114, 344
BsmAI GTCTC 1 cut(s) 457
BsnI GGCC 1 cut(s) 9
Bso31I GGTCTC 1 cut(s) 457
Bsp143I GATC 2 cut(s) 602, 733
BspACI CCGC 4 cut(s) 355, 466, 523, 762
BspANI GGCC 1 cut(s) 9
BspCNI CTCAG 1 cut(s) 454
BspFNI CGCG 1 cut(s) 437
BspLI GGNNCC 2 cut(s) 348, 735
BspMI ACCTGC 1 cut(s) 291
BspPI GGATC 3 cut(s) 610, 728, 741
BspTNI GGTCTC 1 cut(s) 457
BsrBI CCGCTC 2 cut(s) 466, 523
BsrFI RCCGGY 1 cut(s) 148
BsrI ACTGG 2 cut(s) 108, 498
BssAI RCCGGY 1 cut(s) 148
BssECI CCNNGG 2 cut(s) 338, 699
BssMI GATC 2 cut(s) 602, 733
BssT1I CCWWGG 1 cut(s) 699
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 3 cut(s) 272, 658, 715
Bst6I CTCTTC 1 cut(s) 285
BstC8I GCNNGC 2 cut(s) 359, 597
BstDEI CTNAG 2 cut(s) 308, 462
BstFNI CGCG 1 cut(s) 437
BstKTI GATC 2 cut(s) 605, 736
BstMAI GTCTC 1 cut(s) 457
BstMBI GATC 2 cut(s) 602, 733
BstMWI GCNNNNNNNGC 1 cut(s) 536
BstNI CCWGG 1 cut(s) 339
BstPAI GACNNNNGTC 1 cut(s) 449
BstSCI CCNGG 2 cut(s) 9, 337
BstSFI CTRYAG 1 cut(s) 657
BstUI CGCG 1 cut(s) 437
BstV1I GCAGC 6 cut(s) 55, 348, 369, 604, 607, 794
BstV2I GAAGAC 1 cut(s) 239
BstX2I RGATCY 1 cut(s) 733
BstYI RGATCY 1 cut(s) 733
BsuI GTATCC 1 cut(s) 160
BsuRI GGCC 1 cut(s) 9
BtsIMutI CAGTG 1 cut(s) 654
BveI ACCTGC 1 cut(s) 291
Cac8I GCNNGC 2 cut(s) 359, 597
CaiI CAGNNNCTG 1 cut(s) 620
Cfr10I RCCGGY 1 cut(s) 148
Cfr13I GGNCC 3 cut(s) 8, 268, 347
Csp6I GTAC 2 cut(s) 259, 335
CviAII CATG 1 cut(s) 283
CviQI GTAC 2 cut(s) 259, 335
DdeI CTNAG 2 cut(s) 308, 462
DpnI GATC 2 cut(s) 604, 735
DpnII GATC 2 cut(s) 602, 733
DrdI GACNNNNNNGTC 1 cut(s) 404
DseDI GACNNNNNNGTC 1 cut(s) 404
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Eco130I CCWWGG 1 cut(s) 699
Eco31I GGTCTC 1 cut(s) 457
Eco47I GGWCC 2 cut(s) 268, 347
Eco57I CTGAAG 1 cut(s) 597
EcoO109I RGGNCCY 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 337
EcoT14I CCWWGG 1 cut(s) 699
ErhI CCWWGG 1 cut(s) 699
FaeI CATG 1 cut(s) 286
FaiI YATR 6 cut(s) 32, 182, 186, 257, 264, 284
FalI AAGNNNNNCTT 2 cut(s) 179, 211
FatI CATG 1 cut(s) 282
FauI CCCGC 3 cut(s) 362, 459, 755
FblI GTMKAC 1 cut(s) 557
Fnu4HI GCNGC 6 cut(s) 44, 362, 383, 618, 621, 783
Fsp4HI GCNGC 6 cut(s) 44, 362, 383, 618, 621, 783
FspBI CTAG 2 cut(s) 738, 752
GluI GCNGC 6 cut(s) 44, 362, 383, 618, 621, 783
GsaI CCCAGC 1 cut(s) 347
HaeIII GGCC 1 cut(s) 9
HapII CCGG 2 cut(s) 11, 149
Hin1II CATG 1 cut(s) 286
HincII GTYRAC 3 cut(s) 16, 430, 682
HindII GTYRAC 3 cut(s) 16, 430, 682
HindIII AAGCTT 1 cut(s) 313
HinfI GANTC 3 cut(s) 220, 572, 695
HpaI GTTAAC 2 cut(s) 430, 682
HpaII CCGG 2 cut(s) 11, 149
HphI GGTGA 3 cut(s) 163, 389, 400
Hpy166II GTNNAC 4 cut(s) 16, 430, 558, 682
Hpy188I TCNGA 3 cut(s) 276, 463, 694
Hpy188III TCNNGA 4 cut(s) 217, 505, 731, 794
Hpy8I GTNNAC 4 cut(s) 16, 430, 558, 682
HpyAV CCTTC 2 cut(s) 520, 751
HpyCH4III ACNGT 3 cut(s) 272, 658, 715
HpyCH4V TGCA 3 cut(s) 286, 364, 782
HpyF10VI GCNNNNNNNGC 1 cut(s) 536
HpyF3I CTNAG 2 cut(s) 308, 462
Hsp92II CATG 1 cut(s) 286
KspAI GTTAAC 2 cut(s) 430, 682
Kzo9I GATC 2 cut(s) 602, 733
LmnI GCTCC 1 cut(s) 63
Lsp1109I GCAGC 6 cut(s) 55, 348, 369, 604, 607, 794
LweI GCATC 2 cut(s) 192, 538
MaeI CTAG 2 cut(s) 738, 752
MaeIII GTNAC 6 cut(s) 151, 303, 406, 625, 652, 744
MalI GATC 2 cut(s) 604, 735
MbiI CCGCTC 2 cut(s) 466, 523
MboI GATC 2 cut(s) 602, 733
MboII GAAGA 4 cut(s) 244, 302, 524, 603
MfeI CAATTG 1 cut(s) 531
MflI RGATCY 1 cut(s) 733
MluCI AATT 3 cut(s) 531, 567, 706
MmeI TCCRAC 1 cut(s) 697
MnlI CCTC 8 cut(s) 259, 290, 360, 396, 484, 587, 738, 801
MroXI GAANNNNTTC 1 cut(s) 212
MseI TTAA 8 cut(s) 75, 90, 393, 417, 429, 563, 681, 710
MspA1I CMGCKG 1 cut(s) 617
MspI CCGG 2 cut(s) 11, 149
MspR9I CCNGG 2 cut(s) 11, 339
MunI CAATTG 1 cut(s) 531
MvaI CCWGG 1 cut(s) 339
MvnI CGCG 1 cut(s) 437
MwoI GCNNNNNNNGC 1 cut(s) 536
NciI CCSGG 1 cut(s) 11
NdeII GATC 2 cut(s) 602, 733
NlaIII CATG 1 cut(s) 286
NlaIV GGNNCC 2 cut(s) 348, 735
NmuCI GTSAC 3 cut(s) 151, 406, 652
PdmI GAANNNNTTC 1 cut(s) 212
PfeI GAWTC 3 cut(s) 220, 572, 695
PkrI GCNGC 6 cut(s) 45, 363, 384, 619, 622, 784
PpuMI RGGWCCY 1 cut(s) 347
PshAI GACNNNNGTC 1 cut(s) 449
Psp5II RGGWCCY 1 cut(s) 347
Psp6I CCWGG 1 cut(s) 337
PspFI CCCAGC 1 cut(s) 343
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 2 cut(s) 348, 735
PspPI GGNCC 3 cut(s) 8, 268, 347
PspPPI RGGWCCY 1 cut(s) 347
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 1 cut(s) 733
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 2 cut(s) 260, 336
RsaNI GTAC 2 cut(s) 259, 335
SaqAI TTAA 8 cut(s) 75, 90, 393, 417, 429, 563, 681, 710
SatI GCNGC 6 cut(s) 44, 362, 383, 618, 621, 783
Sau3AI GATC 2 cut(s) 602, 733
Sau96I GGNCC 3 cut(s) 8, 268, 347
ScrFI CCNGG 2 cut(s) 11, 339
SfaNI GCATC 2 cut(s) 192, 538
SfcI CTRYAG 1 cut(s) 657
SinI GGWCC 2 cut(s) 268, 347
SmlI CTYRAG 2 cut(s) 481, 792
SmoI CTYRAG 2 cut(s) 481, 792
Sse9I AATT 3 cut(s) 531, 567, 706
SsiI CCGC 4 cut(s) 355, 466, 523, 762
SspI AATATT 1 cut(s) 213
SspMI CTAG 2 cut(s) 738, 752
StyD4I CCNGG 2 cut(s) 9, 337
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 3 cut(s) 272, 658, 715
TasI AATT 3 cut(s) 531, 567, 706
TatI WGTACW 1 cut(s) 258
TfiI GAWTC 3 cut(s) 220, 572, 695
Tru1I TTAA 8 cut(s) 75, 90, 393, 417, 429, 563, 681, 710
Tru9I TTAA 8 cut(s) 75, 90, 393, 417, 429, 563, 681, 710
TscAI CASTG 1 cut(s) 661
TseFI GTSAC 3 cut(s) 151, 406, 652
TseI GCWGC 6 cut(s) 43, 361, 382, 617, 620, 782
Tsp45I GTSAC 3 cut(s) 151, 406, 652
TspDTI ATGAA 1 cut(s) 153
TspRI CASTG 1 cut(s) 661
VpaK11BI GGWCC 2 cut(s) 268, 347
XmiI GTMKAC 1 cut(s) 557
XmnI GAANNNNTTC 1 cut(s) 212
XspI CTAG 2 cut(s) 738, 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.