Rorug04G0213300

YT521-B-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0006149
Physical Location & Seq
Forward (+)
37032272 .. 37034767
2496 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0213300.1

Sequence Viewer

Length: 621 bp
ATGGTGTCCGGGTCAGGGATCTGCGCCAAGCGCGTGGTGGTGGACGCTCGTCACCACATGCTGGGCCGCTTGGCTTCCATTATCGCCAAGGAGCTCCTCAACGGCCAGAAGGTCGTCGTCGTCCGCTGCGAGGAGATCTGCATCTCCGGTGGCTTGGTCAGGCAGAAGATGAAGTACATGAGGTTCTTGCGTAAGCGCATGAACACCAAGCCCTCTCACGGCCCCATTCACTTCCGTGCCCCCGCTAAGATCCTCTGGCGCACAATCCGTGGGATGATTCCACACAAGACAAAGCGTGGAGCTGCTGCGCTTGCTCGTCTGAAGGCCTATGAGGGAATTCCACCTCCTTATGATAAGATTAAGAGGATGGTCATTCCCGATGCTCTCAAGGTCTTGAGGCTTCAAGCAGGACACAAATACTGTTTGTTGGGCAAGCTTTCATCAGAGGTTGGATGGAACCACTATGACACTATCAGGGAGCTTGAGAAGAAGAGGAAGGAGAGGTCTCAGATTGCATATGAGAGGAAGAAACAGCTGAACAAACTGAGAGTCAAAGCAGAAAAGGTTGCTGAGGAGAAACTTGGTCCTCATCTAGAGATCATTGCCCCGATCAAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YTH

Protein Analysis

206

Amino Acids

23.6

Weight (kDa)

10.4

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L13 PF00572 11 - 115 1.1e-22 Ribosomal protein L13
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 61
AccII CGCG 1 cut(s) 33
AciI CCGC 3 cut(s) 67, 124, 243
AclWI GGATC 2 cut(s) 26, 244
AcoI YGGCCR 1 cut(s) 103
AcsI RAATTY 1 cut(s) 336
AcuI CTGAAG 1 cut(s) 341
AfaI GTAC 1 cut(s) 176
AfiI CCNNNNNNNGG 4 cut(s) 15, 61, 130, 218
AgsI TTSAA 1 cut(s) 404
AleI CACNNNNGTG 1 cut(s) 234
AluBI AGCT 5 cut(s) 94, 302, 436, 481, 535
AluI AGCT 5 cut(s) 94, 302, 436, 481, 535
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 510
AlwI GGATC 2 cut(s) 26, 244
AoxI GGCC 4 cut(s) 64, 103, 220, 324
ApeKI GCWGC 3 cut(s) 126, 302, 305
ApoI RAATTY 1 cut(s) 336
ArsI GACNNNNNNTTYG 2 cut(s) 534, 566
AspLEI GCGC 5 cut(s) 26, 33, 198, 261, 310
AspS9I GGNCC 3 cut(s) 64, 221, 584
AsuC2I CCSGG 1 cut(s) 10
AsuHPI GGTGA 1 cut(s) 44
AvaII GGWCC 1 cut(s) 584
BaeGI GKGCMC 1 cut(s) 241
BanII GRGCYC 1 cut(s) 96
BarI GAAGNNNNNNTAC 2 cut(s) 158, 190
Bbv12I GWGCWC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 570
BbvI GCAGC 3 cut(s) 113, 289, 292
BccI CCATC 2 cut(s) 361, 447
BceAI ACGGC 2 cut(s) 118, 235
BcnI CCSGG 1 cut(s) 10
BcoDI GTCTC 1 cut(s) 510
BfaI CTAG 1 cut(s) 593
BglII AGATCT 1 cut(s) 135
BisI GCNGC 4 cut(s) 67, 127, 303, 306
BlsI GCNGC 4 cut(s) 68, 128, 304, 307
Bme1390I CCNGG 1 cut(s) 10
Bme18I GGWCC 1 cut(s) 584
BmgT120I GGNCC 3 cut(s) 64, 221, 584
BmiI GGNNCC 2 cut(s) 223, 458
BmrFI CCNGG 1 cut(s) 10
BmsI GCATC 2 cut(s) 150, 370
BoxI GACNNNNGTC 1 cut(s) 48
Bpu10I CCTNAGC 1 cut(s) 570
BpuEI CTTGAG 3 cut(s) 371, 415, 503
BpuMI CCSGG 1 cut(s) 10
BsaBI GATNNNNATC 1 cut(s) 140
BsaI GGTCTC 1 cut(s) 510
BsaJI CCNNGG 2 cut(s) 87, 268
BsaWI WCCGGW 1 cut(s) 146
Bsc4I CCNNNNNNNGG 4 cut(s) 15, 61, 130, 218
Bse3DI GCAATG 1 cut(s) 600
Bse8I GATNNNNATC 1 cut(s) 140
BseDI CCNNGG 2 cut(s) 87, 268
BseGI GGATG 3 cut(s) 279, 372, 458
BseJI GATNNNNATC 1 cut(s) 140
BseLI CCNNNNNNNGG 4 cut(s) 15, 61, 130, 218
BseMI GCAATG 1 cut(s) 600
BseMII CTCAG 3 cut(s) 521, 536, 561
BseRI GAGGAG 3 cut(s) 86, 146, 587
BseSI GKGCMC 1 cut(s) 241
BseXI GCAGC 3 cut(s) 113, 289, 292
BseYI CCCAGC 1 cut(s) 61
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 4 cut(s) 66, 105, 222, 326
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 2 cut(s) 9, 147
BslI CCNNNNNNNGG 4 cut(s) 15, 61, 130, 218
BsmAI GTCTC 1 cut(s) 510
BsnI GGCC 4 cut(s) 66, 105, 222, 326
Bso31I GGTCTC 1 cut(s) 510
Bsp1286I GDGCHC 2 cut(s) 96, 241
Bsp143I GATC 5 cut(s) 18, 135, 249, 597, 609
BspACI CCGC 3 cut(s) 67, 124, 243
BspANI GGCC 4 cut(s) 66, 105, 222, 326
BspCNI CTCAG 3 cut(s) 520, 537, 562
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 223, 458
BspPI GGATC 2 cut(s) 26, 244
BspTNI GGTCTC 1 cut(s) 510
BsrDI GCAATG 1 cut(s) 600
BssECI CCNNGG 2 cut(s) 87, 268
BssMI GATC 5 cut(s) 18, 135, 249, 597, 609
BssT1I CCWWGG 1 cut(s) 87
Bst4CI ACNGT 1 cut(s) 422
Bst6I CTCTTC 1 cut(s) 485
BstC8I GCNNGC 2 cut(s) 312, 434
BstDEI CTNAG 4 cut(s) 246, 507, 545, 570
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 3 cut(s) 279, 372, 458
BstFNI CGCG 1 cut(s) 33
BstHHI GCGC 5 cut(s) 26, 33, 198, 261, 310
BstKTI GATC 5 cut(s) 21, 138, 252, 600, 612
BstMAI GTCTC 1 cut(s) 510
BstMBI GATC 5 cut(s) 18, 135, 249, 597, 609
BstMWI GCNNNNNNNGC 2 cut(s) 30, 311
BstNSI RCATGY 1 cut(s) 61
BstPAI GACNNNNGTC 1 cut(s) 48
BstSCI CCNGG 1 cut(s) 8
BstSLI GKGCMC 1 cut(s) 241
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 3 cut(s) 113, 289, 292
BstX2I RGATCY 3 cut(s) 18, 135, 249
BstXI CCANNNNNNTGG 1 cut(s) 34
BstYI RGATCY 3 cut(s) 18, 135, 249
BsuRI GGCC 4 cut(s) 66, 105, 222, 326
BtgI CCRYGG 1 cut(s) 268
BtsCI GGATG 3 cut(s) 279, 372, 458
Cac8I GCNNGC 2 cut(s) 312, 434
CfoI GCGC 5 cut(s) 26, 33, 198, 261, 310
Cfr13I GGNCC 3 cut(s) 64, 221, 584
CseI GACGC 1 cut(s) 53
Csp6I GTAC 1 cut(s) 175
CviAII CATG 3 cut(s) 58, 178, 199
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 4 cut(s) 246, 507, 545, 570
DpnI GATC 5 cut(s) 20, 137, 251, 599, 611
DpnII GATC 5 cut(s) 18, 135, 249, 597, 609
EaeI YGGCCR 1 cut(s) 103
Eam1104I CTCTTC 1 cut(s) 485
EarI CTCTTC 1 cut(s) 485
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 87
Eco147I AGGCCT 1 cut(s) 326
Eco24I GRGCYC 1 cut(s) 96
Eco31I GGTCTC 1 cut(s) 510
Eco47I GGWCC 1 cut(s) 584
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 94
EcoRI GAATTC 1 cut(s) 336
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 3 cut(s) 61, 181, 202
FaiI YATR 8 cut(s) 59, 179, 200, 330, 351, 465, 517, 519
FatI CATG 3 cut(s) 57, 177, 198
FauI CCCGC 1 cut(s) 250
FauNDI CATATG 1 cut(s) 517
Fnu4HI GCNGC 4 cut(s) 67, 127, 303, 306
FokI GGATG 3 cut(s) 286, 379, 465
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 4 cut(s) 67, 127, 303, 306
FspBI CTAG 1 cut(s) 593
GlaI GCGC 5 cut(s) 25, 32, 197, 260, 309
GluI GCNGC 4 cut(s) 67, 127, 303, 306
GsaI CCCAGC 1 cut(s) 65
HaeIII GGCC 4 cut(s) 66, 105, 222, 326
HapII CCGG 2 cut(s) 9, 147
HgaI GACGC 1 cut(s) 53
HhaI GCGC 5 cut(s) 26, 33, 198, 261, 310
Hin1II CATG 3 cut(s) 61, 181, 202
Hin6I GCGC 5 cut(s) 24, 31, 196, 259, 308
HinP1I GCGC 5 cut(s) 24, 31, 196, 259, 308
HindIII AAGCTT 1 cut(s) 434
HinfI GANTC 2 cut(s) 277, 549
HpaII CCGG 2 cut(s) 9, 147
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 3 cut(s) 321, 445, 510
Hpy188III TCNNGA 3 cut(s) 377, 394, 593
Hpy8I GTNNAC 1 cut(s) 43
Hpy99I CGWCG 2 cut(s) 119, 122
HpyAV CCTTC 3 cut(s) 103, 316, 490
HpyCH4III ACNGT 1 cut(s) 422
HpyCH4V TGCA 2 cut(s) 141, 515
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 311
HpyF3I CTNAG 4 cut(s) 246, 507, 545, 570
Hsp92II CATG 3 cut(s) 61, 181, 202
HspAI GCGC 5 cut(s) 24, 31, 196, 259, 308
Kzo9I GATC 5 cut(s) 18, 135, 249, 597, 609
LmnI GCTCC 4 cut(s) 91, 99, 299, 478
LpnPI CCDG 8 cut(s) 22, 47, 119, 145, 160, 241, 393, 460
Lsp1109I GCAGC 3 cut(s) 113, 289, 292
LweI GCATC 2 cut(s) 150, 370
MaeI CTAG 1 cut(s) 593
MaeIII GTNAC 1 cut(s) 50
MalI GATC 5 cut(s) 20, 137, 251, 599, 611
MboI GATC 5 cut(s) 18, 135, 249, 597, 609
MboII GAAGA 4 cut(s) 178, 499, 502, 538
MflI RGATCY 3 cut(s) 18, 135, 249
MhlI GDGCHC 2 cut(s) 96, 241
MluCI AATT 1 cut(s) 336
MlyI GAGTC 1 cut(s) 558
MmeI TCCRAC 1 cut(s) 430
MseI TTAA 1 cut(s) 360
MslI CAYNNNNRTG 1 cut(s) 234
MspA1I CMGCKG 2 cut(s) 126, 535
MspI CCGG 2 cut(s) 9, 147
MspR9I CCNGG 1 cut(s) 10
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 2 cut(s) 30, 311
NciI CCSGG 1 cut(s) 10
NdeI CATATG 1 cut(s) 517
NdeII GATC 5 cut(s) 18, 135, 249, 597, 609
NlaIII CATG 3 cut(s) 61, 181, 202
NlaIV GGNNCC 2 cut(s) 223, 458
NmuCI GTSAC 1 cut(s) 50
NspI RCATGY 1 cut(s) 61
OliI CACNNNNGTG 1 cut(s) 234
PceI AGGCCT 1 cut(s) 326
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 1 cut(s) 277
PflMI CCANNNNNTGG 1 cut(s) 61
PkrI GCNGC 4 cut(s) 68, 128, 304, 307
PleI GAGTC 1 cut(s) 557
PpsI GAGTC 1 cut(s) 557
PshAI GACNNNNGTC 1 cut(s) 48
Psp124BI GAGCTC 1 cut(s) 96
PspFI CCCAGC 1 cut(s) 61
PspN4I GGNNCC 2 cut(s) 223, 458
PspPI GGNCC 3 cut(s) 64, 221, 584
PsrI GAACNNNNNNTAC 2 cut(s) 167, 199
PsuI RGATCY 3 cut(s) 18, 135, 249
PvuII CAGCTG 1 cut(s) 535
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
RseI CAYNNNNRTG 1 cut(s) 234
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 1 cut(s) 360
SatI GCNGC 4 cut(s) 67, 127, 303, 306
Sau3AI GATC 5 cut(s) 18, 135, 249, 597, 609
Sau96I GGNCC 3 cut(s) 64, 221, 584
SchI GAGTC 1 cut(s) 558
ScrFI CCNGG 1 cut(s) 10
SduI GDGCHC 2 cut(s) 96, 241
SfaNI GCATC 2 cut(s) 150, 370
SinI GGWCC 1 cut(s) 584
SmiMI CAYNNNNRTG 1 cut(s) 234
SmlI CTYRAG 3 cut(s) 386, 394, 482
SmoI CTYRAG 3 cut(s) 386, 394, 482
Sse9I AATT 1 cut(s) 336
SseBI AGGCCT 1 cut(s) 326
SsiI CCGC 3 cut(s) 67, 124, 243
SspMI CTAG 1 cut(s) 593
SstI GAGCTC 1 cut(s) 96
StuI AGGCCT 1 cut(s) 326
StyD4I CCNGG 1 cut(s) 8
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 422
TasI AATT 1 cut(s) 336
TatI WGTACW 1 cut(s) 174
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 1 cut(s) 277
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TseFI GTSAC 1 cut(s) 50
TseI GCWGC 3 cut(s) 126, 302, 305
Tsp45I GTSAC 1 cut(s) 50
TspDTI ATGAA 3 cut(s) 185, 215, 429
TspGWI ACGGA 2 cut(s) 224, 257
Van91I CCANNNNNTGG 1 cut(s) 61
VpaK11BI GGWCC 1 cut(s) 584
XapI RAATTY 1 cut(s) 336
XbaI TCTAGA 1 cut(s) 592
XceI RCATGY 1 cut(s) 61
XcmI CCANNNNNNNNNTGG 1 cut(s) 34
XspI CTAG 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.