pycom13g13830

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0013792
Physical Location & Seq
Reverse (-)
9832299 .. 9839205
6907 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3375 bp
ATGTCGTCTTTGAGCAGAGAACTGGTGTTCCTGATCCTCCAATTTCTGGAGGAAGAGAAGTTCAAGGAGTCCGTACACAGGCTGGAGCAGGAATCGGGGTACTTCTTCAACATGAAGTACTTTGAGGAGAAGGCACTGGCTGGAGAGTGGGACGAGGTCGAGAAGTACCTCTCTGGGTTCACCAAGGTCGATGAGAATCGCTATTCGATGAAGATTTTCTTCGAGGTTCGAAAGCAGAAGTATCTCGAGGCGCTCGACAGGAATGACAGAGCTAAGGCTGTTGAGATACTGGTGAAGGATTTGAAAGTGTTCTCGACGTTTAACGAAGAGTTGTACAAGGAAATTACGCATCTTTTGACCCTCGAAAACTTCAGAGAAAATGAGCAGTTATCGAAATATGGTGATACTAAGTCGGCTAGAAGCATAATGCTAGTAGAGCTTAAAAAGTTGATAGAAGCAAATCCGCTTTTCCGAGATAAGCTTGCTTTACCCACTTTGAAGGCTTCACGCTTGCGAACTCTAATTAATCAAAGTCTAAATTGGCAACACCAGCTGTGCAAGAACCCAAGGCCAAATCCTGATATTAAGACCTTATTCATGGACCACTCGTGTTCTCCAACAAATGGGGCTCGTGCATCCACGCCAGTTACTCTTCCGGTTGCAGCTCTTGCAAAGCCAACTTATGCTCCTCTAGGAGCACATGGTGGAGCTTTTCCGCCGGCTGCTGCAGCTGCAGCTAATGCAAATTTAGCAGGATGGATGTCGAATGCTAATCCTTCCTTGTCTGTGCAATCAGCTGTTGTTGCCGCTTCACCCTTCCCTGTTCGACCCAGTCAAGTTTCTGTTTTAAAGCATCCAAGAACACCCTCAAATGCTCTTGGAATGAGCGATTATCAAAGCTCAGATCACGAGCAACTAATGAAACGCCTGCGGCCTGCACAATCTGTTGATGAGGTCAGTTATCCTCCCCCTCCCCAGCATGCTTCCTGGTCACTAGATGACCTACCGAGGACTTTAGCGTGCACTCTTCGTCAGGGATTCAATGTGATGAGCATGGATTTCCACCCTTCTCATCACACATTACTTGCTGTTGGATGTAGTAATGGCGAAATTACACTGTGGGAAGTTGGGATACGAGAGAAGCTAGTGTCAAAGCCATTCAAAGTATGGAATATGACTGCTTGTTCTAATGCATTTCAGGTTGCTATGGTCAAAGATCGATCAATATCTGTAAGCCGTGTATCATGGAATCAAGATGGAAGCTTAATTGGTGTTGCATTTACCAAACACTTGGTTCATTTGTACGCTTATCAAGGACCAACTGATCTACGGCAACATTTGGAGATTGATGCTCACAGTGGCAGTGTGAATGACTTAGCCTTTTCTCATCCAAACAAGCAATTATGTCTTATAACTTGTGGGGAGGATAAGCTGATAAAGGTTTGGGATTTGGGTGGAAGAATTCTATTCAGCTTTGAAGGTCATGAAGCGCCTGTTTACTCAATTTGCCCCCACCAGAAGGAGAATATTCAGTTCATATTTTCAACTGCTGTTGATGGGAAAATAAAAGCTTGGCTGTATGACAATGTGGGCTCCAGGGTTGACTATGATGCTCCTGGGCAGTGGTGCACCACAATGCTCTACAGTGATGACGGAAATAGATTATTCTCTTGTGGGACTAGTAAAGATGGTGATTCTTTCCTCGTTGAATGGAATGAAAGTGAAGGGGCAATAAAGAGGACATATAATGGATTCAGAAAAAAATCTTCTGGCATAGTGCAATTTGATACAACAAAAAATCACTTTTTGGCTGTTGGTGAAGATAATCAGATAAAGTTTTGGGATATGGATAATACAAATGTTCTTACTGGTACAGATGCTGAGGGTGGACTTCTGACTCTTCCCCGCTTGAGATTCAATAAGGAAGGAAATTTGCTTGCTGCTACCACGTCTGACAACGGAATCAAAATTCTTGCTAATGCTGAGGGTCTAAGATCCTTAAGAGCAATTGAAACTCGATCTTATGAAGCATCCAGAGGACCTATTGAGATGAAGGTATCTGGTTCTTCCATGGTTCCGAATGTCAACCCAACCATTAATAAAGTGGACCGTATGGATACAAGCTCTCCTGCCAGACCGACCCATATTCTTTCTATGGCAAGAGCCGATATGGCAAGAAGCCTGGAAAAGAGAAGAAGCTTAGATGATGTATCTGACAAGATTAAACGTTGGGAACTGGCGGAGATTGTAGATCCCGTTCAGTGTCGAGTGGCTACTATGCCAGAAAGTAAAGATCCTGCTAACAAGGTTGCTCGACTTCTGTATACAAATTCTGGTTCTGGCATTCTGGCTCTCGGTTCAAATGGGGTGCAGAAGCTATGGAAGTGGAGCCGCAATGAACAAAATCTAAGTGGGAAGGCCACAGCAAGTGTTGTTCCCCAACATTGGCAACCCAACAGTGGTCTTTTAATGACTAATGATGTCTCAGAAAATGTTGAAGAAGCGGTTCCATGCATTGCACTCTCAAAAAATGACTCTTATGTGATGTCAGCCTGTGGAGGAAAAGTGTCTCTGTTCAATATGATGACTTTTAAGGTAATGACAACTTTCATGCCACCTCCACCAGTGTCAACCTACTTGTCATTTCATCCTCTGGATAATAATATCATAGCAATAGGAATGGAAGATTCAACTATCCACATTTACAATGTTCGACTTGATGAGGTCAAAGCAAAACTGAAAGGCCACCAGAAGCACATTACTGGTTTAGCATTTTCTGTCAACCATAAACTCATGGTTTCATCAGGTGCCGATGCTCAACTATGCTTTTGGAACATGGATACATGGGATAAGAGGAAATCGGTTCCACTTCAGTTGCCCGCTGGAAAGGCGCCTGTCGGTGACACGCATGTGCAATTTTATTCTGATCAAGTCCGATTGTTGGTATACCATGAGACTCAACTAGCATTATATGATGCGGCCAAATCAGAGTGCATTCGGCAGTGGATGCCACAAGATGTTCTGCCTGCGCCCATTTCTTGTGCAGCATATTCTTCAAGTAGTCAACTAGTTTATGCAGCATTTACTGATGGTAACATTGGAGTATTTGATGCTGATAGTCTGAAACTAAGATGCCGTATTGCCATGTCGGTGTACTTACCACAAGCATCATCAAACAGCCCATCTGTGTACCCGCTGGCTCTTACAGCACATTTGCAGGAGCCGTACCAATTCGCTGTTGGACTGACAGATGGATCTGTTAAAGTTATAGAGCCCTCGGAATCTGAGGGGAGGTGGGGAGTTTTGGTGCCTGTTGATAACGGAACACAGAACGGGTGGACAGCAACATCTTCGAGTAACAATCCAGCATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

1125

Amino Acids

124.85

Weight (kDa)

6.87

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 5 - 33 3.4e-11 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 1.1e-61 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 2.8e-25 TOPLESS, zinc finger domain
Beta-prop_EML_2 PF23414 330 - 466 1e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 341 - 559 1.4e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 348 - 562 1.3e-08 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 388 - 560 2.8e-13 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 388 - 484 1.4e-07 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 407 - 621 2.8e-18 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 410 - 663 2.4e-21 THOC3 beta-propeller domain
WD40 PF00400 448 - 483 6.6e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 455 - 528 1e-06 WDHD1 first WD40 domain
WDR55 PF24796 496 - 657 7.6e-07 WDR55
Beta-prop_WDR3_1st PF25173 519 - 657 2.7e-06 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 814 - 997 1.7e-14 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 816 - 952 3.6e-12 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 827 - 1042 3e-15 WDHD1 first WD40 domain
WDR55 PF24796 827 - 947 5.3e-10 WDR55
Beta-prop_WDR3_1st PF25173 829 - 951 2.6e-15 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 830 - 953 2.8e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 833 - 1040 3.2e-17 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 833 - 948 1.2e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 836 - 954 1.7e-12 WDR3 second beta-propeller domain
Beta-prop_IFT140_1st PF23383 849 - 953 3.1e-09 IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 853 - 948 8.1e-11 WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 856 - 947 2.8e-08 WDR90/POC16, second beta-propeller
WD40_Gbeta PF25391 878 - 1038 6.5e-06 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 887 - 1045 7.6e-10 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013792)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16830
fragaria_vesca FvH4_4g18910 FvH4_4g18910
malus_domestica MD13G1162100.v1.1 MD16G1161900.v1.1
prunus_persica Prupe.1G129400_v2.0.a1
pyrus_communis pycom13g13830
rosa_chinensis RchiOBHm_Chr4g0423811
rosa_laevigata RLG00000007530
rosa_multiflora Rmu_sc0003369.1_g000018
rosa_roxburghii Rroxscaffold_5G00365920
rosa_rugosa Rorug04G0190900
rosa_samantha Rh4AG250300 Rh4BG255600 Rh4CG263700 Rh4DG250000
rosa_wichuraiana Rw4G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1412
AccB1I GGYRCC 3 cut(s) 2807, 2890, 3307
AccB7I CCANNNNNTGG 2 cut(s) 46, 623
AccI GTMKAC 2 cut(s) 2324, 2946
AclI AACGTT 1 cut(s) 2227
AclWI GGATC 5 cut(s) 28, 1989, 2246, 2288, 3262
AcoI YGGCCR 1 cut(s) 2979
AcsI RAATTY 5 cut(s) 745, 1461, 1930, 1968, 2329
AcuI CTGAAG 2 cut(s) 355, 2855
AcyI GRCGYC 1 cut(s) 2891
AdeI CACNNNGTG 1 cut(s) 704
AfiI CCNNNNNNNGG 7 cut(s) 46, 78, 623, 1519, 2445, 2459, 2941
AflII CTTAAG 1 cut(s) 1999
AhlI ACTAGT 2 cut(s) 1679, 3067
AjiI CACGTC 1 cut(s) 1950
AjnI CCWGG 4 cut(s) 986, 1595, 1615, 2181
AleI CACNNNNGTG 1 cut(s) 2909
AloI GAACNNNNNNTCC 4 cut(s) 12, 44, 44, 76
Alw21I GWGCWC 3 cut(s) 700, 1025, 1631
Alw26I GTCTC 3 cut(s) 2488, 2574, 2948
Alw44I GTGCAC 2 cut(s) 1021, 1627
AlwI GGATC 5 cut(s) 28, 1989, 2246, 2288, 3262
AlwNI CAGNNNCTG 1 cut(s) 1880
Ama87I CYCGRG 1 cut(s) 245
AoxI GGCC 5 cut(s) 569, 932, 2418, 2743, 2979
ApaLI GTGCAC 2 cut(s) 1021, 1627
ApeKI GCWGC 9 cut(s) 662, 722, 725, 728, 731, 734, 1940, 3044, 3077
ApoI RAATTY 5 cut(s) 745, 1461, 1930, 1968, 2329
AseI ATTAAT 2 cut(s) 525, 2097
Asp700I GAANNNNTTC 3 cut(s) 215, 308, 2505
AspLEI GCGC 4 cut(s) 253, 1492, 2893, 3031
AspS9I GGNCC 4 cut(s) 601, 1316, 2039, 2107
AsuHPI GGTGA 7 cut(s) 172, 304, 413, 804, 1703, 1829, 2912
AsuII TTCGAA 1 cut(s) 229
AvaI CYCGRG 1 cut(s) 245
AvaII GGWCC 4 cut(s) 601, 1316, 2039, 2107
BaeGI GKGCMC 2 cut(s) 1025, 1631
BanI GGYRCC 3 cut(s) 2807, 2890, 3307
BanII GRGCYC 3 cut(s) 631, 1595, 3276
BarI GAAGNNNNNNTAC 2 cut(s) 1116, 1148
BauI CACGAG 3 cut(s) 607, 630, 908
Bbv12I GWGCWC 3 cut(s) 700, 1025, 1631
BbvCI CCTCAGC 2 cut(s) 1881, 1983
BbvI GCAGC 9 cut(s) 674, 709, 712, 718, 740, 746, 1927, 3056, 3089
BccI CCATC 7 cut(s) 750, 1250, 1550, 1682, 3083, 3190, 3245
BceAI ACGGC 4 cut(s) 1221, 1346, 3120, 3208
BcgI CGANNNNNNTGC 2 cut(s) 3333, 3367
BciT130I CCWGG 4 cut(s) 988, 1597, 1617, 2183
BciVI GTATCC 3 cut(s) 1125, 2110, 2833
BclI TGATCA 1 cut(s) 2926
BcoDI GTCTC 3 cut(s) 2488, 2574, 2948
BcuI ACTAGT 2 cut(s) 1679, 3067
BfaI CTAG 8 cut(s) 417, 431, 692, 995, 1145, 1680, 2963, 3068
BfmI CTRYAG 3 cut(s) 726, 732, 1642
BfoI RGCGCY 3 cut(s) 254, 1493, 2894
BfrI CTTAAG 1 cut(s) 1999
BfuI GTATCC 3 cut(s) 1125, 2110, 2833
BglI GCCNNNNNGGC 1 cut(s) 2171
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 4 cut(s) 988, 1597, 1617, 2183
Bme18I GGWCC 4 cut(s) 601, 1316, 2039, 2107
BmeT110I CYCGRG 1 cut(s) 245
BmgBI CACGTC 1 cut(s) 1950
BmgT120I GGNCC 4 cut(s) 601, 1316, 2039, 2107
BmiI GGNNCC 9 cut(s) 1594, 2076, 2390, 2508, 2809, 2865, 2892, 3222, 3309
BmrFI CCNGG 4 cut(s) 988, 1597, 1617, 2183
BmrI ACTGGG 1 cut(s) 825
BmuI ACTGGG 1 cut(s) 825
BpmI CTGGAG 4 cut(s) 68, 104, 162, 1579
Bpu10I CCTNAGC 3 cut(s) 273, 1881, 1983
Bpu14I TTCGAA 1 cut(s) 229
BpuEI CTTGAG 1 cut(s) 1930
Bsa29I ATCGAT 1 cut(s) 1219
BsaBI GATNNNNATC 2 cut(s) 195, 1225
BsaHI GRCGYC 1 cut(s) 2891
BsaJI CCNNGG 7 cut(s) 183, 566, 1007, 1596, 1616, 2070, 3276
BsaWI WCCGGW 1 cut(s) 655
BsaXI ACNNNNNCTCC 8 cut(s) 670, 700, 2110, 2140, 2550, 2580, 3291, 3321
Bsc4I CCNNNNNNNGG 7 cut(s) 46, 78, 623, 1519, 2445, 2459, 2941
Bse118I RCCGGY 1 cut(s) 718
Bse1I ACTGG 9 cut(s) 27, 141, 294, 644, 831, 1873, 2241, 2624, 2767
Bse3DI GCAATG 2 cut(s) 2401, 2514
Bse8I GATNNNNATC 2 cut(s) 195, 1225
BseBI CCWGG 4 cut(s) 988, 1597, 1617, 2183
BseCI ATCGAT 1 cut(s) 1219
BseDI CCNNGG 7 cut(s) 183, 566, 1007, 1596, 1616, 2070, 3276
BseGI GGATG 9 cut(s) 635, 761, 765, 853, 1100, 1387, 2030, 2647, 3012
BseJI GATNNNNATC 2 cut(s) 195, 1225
BseLI CCNNNNNNNGG 7 cut(s) 46, 78, 623, 1519, 2445, 2459, 2941
BseMI GCAATG 2 cut(s) 2401, 2514
BseMII CTCAG 5 cut(s) 915, 1872, 1974, 2499, 3276
BseNI ACTGG 9 cut(s) 27, 141, 294, 644, 831, 1873, 2241, 2624, 2767
BseRI GAGGAG 2 cut(s) 140, 678
BseSI GKGCMC 2 cut(s) 1025, 1631
BseXI GCAGC 9 cut(s) 674, 709, 712, 718, 740, 746, 1927, 3056, 3089
BseYI CCCAGC 1 cut(s) 975
BsgI GTGCAG 3 cut(s) 921, 2390, 3063
BshFI GGCC 5 cut(s) 571, 934, 2420, 2745, 2981
BshNI GGYRCC 3 cut(s) 2807, 2890, 3307
BshVI ATCGAT 1 cut(s) 1219
BsiHKAI GWGCWC 3 cut(s) 700, 1025, 1631
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 2 cut(s) 656, 719
BslFI GGGAC 2 cut(s) 164, 1690
BslI CCNNNNNNNGG 7 cut(s) 46, 78, 623, 1519, 2445, 2459, 2941
BsmAI GTCTC 3 cut(s) 2488, 2574, 2948
BsmFI GGGAC 2 cut(s) 164, 1690
BsmI GAATGC 3 cut(s) 772, 2343, 2994
BsnI GGCC 5 cut(s) 571, 934, 2420, 2745, 2981
BsoBI CYCGRG 1 cut(s) 245
Bsp119I TTCGAA 1 cut(s) 229
Bsp1286I GDGCHC 6 cut(s) 631, 700, 1025, 1595, 1631, 3276
Bsp1407I TGTACA 1 cut(s) 333
Bsp19I CCATGG 1 cut(s) 2070
BspANI GGCC 5 cut(s) 571, 934, 2420, 2745, 2981
BspCNI CTCAG 5 cut(s) 914, 1873, 1975, 2498, 3277
BspDI ATCGAT 1 cut(s) 1219
BspHI TCATGA 1 cut(s) 1483
BspLI GGNNCC 9 cut(s) 1594, 2076, 2390, 2508, 2809, 2865, 2892, 3222, 3309
BspMAI CTGCAG 2 cut(s) 730, 736
BspPI GGATC 5 cut(s) 28, 1989, 2246, 2288, 3262
BspT104I TTCGAA 1 cut(s) 229
BspT107I GGYRCC 3 cut(s) 2807, 2890, 3307
BspTI CTTAAG 1 cut(s) 1999
BsrDI GCAATG 2 cut(s) 2401, 2514
BsrFI RCCGGY 1 cut(s) 718
BsrGI TGTACA 1 cut(s) 333
BsrI ACTGG 9 cut(s) 27, 141, 294, 644, 831, 1873, 2241, 2624, 2767
BssAI RCCGGY 1 cut(s) 718
BssECI CCNNGG 7 cut(s) 183, 566, 1007, 1596, 1616, 2070, 3276
BssNAI GTATAC 2 cut(s) 2325, 2947
BssNI GRCGYC 1 cut(s) 2891
BssSI CACGAG 3 cut(s) 607, 630, 908
BssT1I CCWWGG 3 cut(s) 183, 566, 2070
Bst1107I GTATAC 2 cut(s) 2325, 2947
Bst2BI CACGAG 3 cut(s) 607, 630, 908
Bst2UI CCWGG 4 cut(s) 988, 1597, 1617, 2183
Bst4CI ACNGT 5 cut(s) 1119, 1358, 1646, 2111, 2459
Bst6I CTCTTC 5 cut(s) 48, 321, 657, 1032, 1905
BstACI GRCGYC 1 cut(s) 2891
BstAFI CTTAAG 1 cut(s) 1999
BstAPI GCANNNNNTGC 3 cut(s) 668, 740, 3007
BstAUI TGTACA 1 cut(s) 333
BstBI TTCGAA 1 cut(s) 229
BstDSI CCRYGG 1 cut(s) 2070
BstF5I GGATG 9 cut(s) 635, 761, 765, 853, 1100, 1387, 2030, 2647, 3012
BstH2I RGCGCY 3 cut(s) 254, 1493, 2894
BstHHI GCGC 4 cut(s) 253, 1492, 2893, 3031
BstMAI GTCTC 3 cut(s) 2488, 2574, 2948
BstNI CCWGG 4 cut(s) 988, 1597, 1617, 2183
BstNSI RCATGY 2 cut(s) 983, 2912
BstSCI CCNGG 4 cut(s) 986, 1595, 1615, 2181
BstSFI CTRYAG 3 cut(s) 726, 732, 1642
BstSLI GKGCMC 2 cut(s) 1025, 1631
BstV1I GCAGC 9 cut(s) 674, 709, 712, 718, 740, 746, 1927, 3056, 3089
BstX2I RGATCY 4 cut(s) 1994, 2251, 2293, 3254
BstXI CCANNNNNNTGG 1 cut(s) 1291
BstYI RGATCY 4 cut(s) 1994, 2251, 2293, 3254
BstZ17I GTATAC 2 cut(s) 2325, 2947
Bsu15I ATCGAT 1 cut(s) 1219
BsuI GTATCC 3 cut(s) 1125, 2110, 2833
BsuRI GGCC 5 cut(s) 571, 934, 2420, 2745, 2981
BsuTUI ATCGAT 1 cut(s) 1219
BtgI CCRYGG 1 cut(s) 2070
BtrI CACGTC 1 cut(s) 1950
BtsCI GGATG 9 cut(s) 635, 761, 765, 853, 1100, 1387, 2030, 2647, 3012
BtsI GCAGTG 3 cut(s) 1369, 1628, 3008
CaiI CAGNNNCTG 1 cut(s) 1880
CciI TCATGA 1 cut(s) 1483
CfoI GCGC 4 cut(s) 253, 1492, 2893, 3031
Cfr10I RCCGGY 1 cut(s) 718
Cfr13I GGNCC 4 cut(s) 601, 1316, 2039, 2107
ClaI ATCGAT 1 cut(s) 1219
CspCI CAANNNNNGTGG 2 cut(s) 2856, 2891
DinI GGCGCC 1 cut(s) 2892
DraI TTTAAA 1 cut(s) 849
DraIII CACNNNGTG 1 cut(s) 704
EaeI YGGCCR 1 cut(s) 2979
Eam1104I CTCTTC 5 cut(s) 48, 321, 657, 1032, 1905
EarI CTCTTC 5 cut(s) 48, 321, 657, 1032, 1905
EciI GGCGGA 2 cut(s) 705, 2255
Eco130I CCWWGG 3 cut(s) 183, 566, 2070
Eco24I GRGCYC 3 cut(s) 631, 1595, 3276
Eco47I GGWCC 4 cut(s) 601, 1316, 2039, 2107
Eco57I CTGAAG 2 cut(s) 355, 2855
Eco88I CYCGRG 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 2039
EcoRI GAATTC 1 cut(s) 1461
EcoRII CCWGG 4 cut(s) 986, 1595, 1615, 2181
EcoT14I CCWWGG 3 cut(s) 183, 566, 2070
EcoT22I ATGCAT 2 cut(s) 1195, 2516
EcoT38I GRGCYC 3 cut(s) 631, 1595, 3276
EgeI GGCGCC 1 cut(s) 2892
EheI GGCGCC 1 cut(s) 2892
ErhI CCWWGG 3 cut(s) 183, 566, 2070
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FaqI GGGAC 2 cut(s) 164, 1690
FauI CCCGC 3 cut(s) 1913, 2887, 3201
FbaI TGATCA 1 cut(s) 2926
FblI GTMKAC 2 cut(s) 2324, 2946
FokI GGATG 9 cut(s) 622, 768, 772, 840, 1107, 1374, 2017, 2634, 3019
FriOI GRGCYC 3 cut(s) 631, 1595, 3276
FspBI CTAG 8 cut(s) 417, 431, 692, 995, 1145, 1680, 2963, 3068
GlaI GCGC 4 cut(s) 252, 1491, 2892, 3030
GsaI CCCAGC 1 cut(s) 979
GsuI CTGGAG 4 cut(s) 68, 104, 162, 1579
HaeII RGCGCY 3 cut(s) 254, 1493, 2894
HaeIII GGCC 5 cut(s) 571, 934, 2420, 2745, 2981
HapII CCGG 2 cut(s) 656, 719
HhaI GCGC 4 cut(s) 253, 1492, 2893, 3031
Hin1I GRCGYC 1 cut(s) 2891
Hin6I GCGC 4 cut(s) 251, 1490, 2891, 3029
HinP1I GCGC 4 cut(s) 251, 1490, 2891, 3029
HincII GTYRAC 5 cut(s) 1603, 2086, 2631, 2782, 3065
HindII GTYRAC 5 cut(s) 1603, 2086, 2631, 2782, 3065
HindIII AAGCTT 4 cut(s) 479, 1261, 1569, 2197
HpaII CCGG 2 cut(s) 656, 719
HphI GGTGA 7 cut(s) 172, 304, 413, 804, 1703, 1829, 2912
Hpy99I CGWCG 1 cut(s) 319
HpyCH4III ACNGT 5 cut(s) 1119, 1358, 1646, 2111, 2459
HpyCH4IV ACGT 3 cut(s) 317, 1949, 2227
HpySE526I ACGT 3 cut(s) 317, 1949, 2227
Hsp92I GRCGYC 1 cut(s) 2891
HspAI GCGC 4 cut(s) 251, 1490, 2891, 3029
KasI GGCGCC 1 cut(s) 2890
KroI GCCGGC 1 cut(s) 718
KroNI GCCGGC 1 cut(s) 720
Ksp22I TGATCA 1 cut(s) 2926
LmnI GCTCC 8 cut(s) 85, 691, 695, 707, 1598, 1618, 2388, 3220
Lsp1109I GCAGC 9 cut(s) 674, 709, 712, 718, 740, 746, 1927, 3056, 3089
MaeI CTAG 8 cut(s) 417, 431, 692, 995, 1145, 1680, 2963, 3068
MaeII ACGT 3 cut(s) 317, 1949, 2227
MaeIII GTNAC 5 cut(s) 646, 990, 2900, 3092, 3356
MfeI CAATTG 1 cut(s) 2007
MflI RGATCY 4 cut(s) 1994, 2251, 2293, 3254
MhlI GDGCHC 6 cut(s) 631, 700, 1025, 1595, 1631, 3276
Mly113I GGCGCC 1 cut(s) 2891
MlyI GAGTC 4 cut(s) 77, 1891, 2528, 2950
MmeI TCCRAC 3 cut(s) 641, 1072, 3220
Mph1103I ATGCAT 2 cut(s) 1195, 2516
MroNI GCCGGC 1 cut(s) 718
MroXI GAANNNNTTC 3 cut(s) 215, 308, 2505
MslI CAYNNNNRTG 3 cut(s) 1634, 2909, 3149
MspA1I CMGCKG 5 cut(s) 553, 731, 797, 2882, 3196
MspCI CTTAAG 1 cut(s) 1999
MspI CCGG 2 cut(s) 656, 719
MspR9I CCNGG 4 cut(s) 988, 1597, 1617, 2183
MunI CAATTG 1 cut(s) 2007
Mva1269I GAATGC 3 cut(s) 772, 2343, 2994
MvaI CCWGG 4 cut(s) 988, 1597, 1617, 2183
NaeI GCCGGC 1 cut(s) 720
NarI GGCGCC 1 cut(s) 2891
NcoI CCATGG 1 cut(s) 2070
NgoMIV GCCGGC 1 cut(s) 718
NlaIV GGNNCC 9 cut(s) 1594, 2076, 2390, 2508, 2809, 2865, 2892, 3222, 3309
NmuCI GTSAC 2 cut(s) 990, 2900
NsiI ATGCAT 2 cut(s) 1195, 2516
NspI RCATGY 2 cut(s) 983, 2912
NspV TTCGAA 1 cut(s) 229
OliI CACNNNNGTG 1 cut(s) 2909
PaeI GCATGC 1 cut(s) 983
PaeR7I CTCGAG 1 cut(s) 245
PagI TCATGA 1 cut(s) 1483
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 3 cut(s) 772, 2343, 2994
PdiI GCCGGC 1 cut(s) 720
PdmI GAANNNNTTC 3 cut(s) 215, 308, 2505
PflFI GACNNNGTC 2 cut(s) 155, 831
PflMI CCANNNNNTGG 2 cut(s) 46, 623
PleI GAGTC 4 cut(s) 76, 1891, 2528, 2950
PluTI GGCGCC 1 cut(s) 2894
PpsI GAGTC 4 cut(s) 76, 1891, 2528, 2950
PpuMI RGGWCCY 1 cut(s) 2039
PshBI ATTAAT 2 cut(s) 525, 2097
PsiI TTATAA 1 cut(s) 1412
Psp1406I AACGTT 1 cut(s) 2227
Psp5II RGGWCCY 1 cut(s) 2039
Psp6I CCWGG 4 cut(s) 986, 1595, 1615, 2181
PspFI CCCAGC 1 cut(s) 975
PspGI CCWGG 4 cut(s) 986, 1595, 1615, 2181
PspN4I GGNNCC 9 cut(s) 1594, 2076, 2390, 2508, 2809, 2865, 2892, 3222, 3309
PspPI GGNCC 4 cut(s) 601, 1316, 2039, 2107
PspPPI RGGWCCY 1 cut(s) 2039
PsrI GAACNNNNNNTAC 2 cut(s) 2241, 2273
PstI CTGCAG 2 cut(s) 730, 736
PstNI CAGNNNCTG 1 cut(s) 1880
PsuI RGATCY 4 cut(s) 1994, 2251, 2293, 3254
PsyI GACNNNGTC 2 cut(s) 155, 831
PvuII CAGCTG 3 cut(s) 553, 731, 797
RseI CAYNNNNRTG 3 cut(s) 1634, 2909, 3149
Sau96I GGNCC 4 cut(s) 601, 1316, 2039, 2107
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 4 cut(s) 77, 1891, 2528, 2950
ScrFI CCNGG 4 cut(s) 988, 1597, 1617, 2183
SduI GDGCHC 6 cut(s) 631, 700, 1025, 1595, 1631, 3276
SfcI CTRYAG 3 cut(s) 726, 732, 1642
SfoI GGCGCC 1 cut(s) 2892
Sfr274I CTCGAG 1 cut(s) 245
SfuI TTCGAA 1 cut(s) 229
SinI GGWCC 4 cut(s) 601, 1316, 2039, 2107
SlaI CTCGAG 1 cut(s) 245
SmiMI CAYNNNNRTG 3 cut(s) 1634, 2909, 3149
SmlI CTYRAG 3 cut(s) 245, 1909, 1999
SmoI CTYRAG 3 cut(s) 245, 1909, 1999
SpeI ACTAGT 2 cut(s) 1679, 3067
SphI GCATGC 1 cut(s) 983
SspDI GGCGCC 1 cut(s) 2890
SspI AATATT 1 cut(s) 1528
SspMI CTAG 8 cut(s) 417, 431, 692, 995, 1145, 1680, 2963, 3068
StyD4I CCNGG 4 cut(s) 986, 1595, 1615, 2181
StyI CCWWGG 3 cut(s) 183, 566, 2070
TaaI ACNGT 5 cut(s) 1119, 1358, 1646, 2111, 2459
TaiI ACGT 3 cut(s) 320, 1952, 2230
TaqII GACCGA 1 cut(s) 2152
TatI WGTACW 3 cut(s) 117, 333, 3153
TauI GCSGC 4 cut(s) 809, 934, 2394, 2981
TseFI GTSAC 2 cut(s) 990, 2900
TseI GCWGC 9 cut(s) 662, 722, 725, 728, 731, 734, 1940, 3044, 3077
Tsp45I GTSAC 2 cut(s) 990, 2900
TspGWI ACGGA 4 cut(s) 61, 1668, 1974, 3336
Tth111I GACNNNGTC 2 cut(s) 155, 831
Van91I CCANNNNNTGG 2 cut(s) 46, 623
Vha464I CTTAAG 1 cut(s) 1999
VneI GTGCAC 2 cut(s) 1021, 1627
VpaK11BI GGWCC 4 cut(s) 601, 1316, 2039, 2107
VspI ATTAAT 2 cut(s) 525, 2097
XapI RAATTY 5 cut(s) 745, 1461, 1930, 1968, 2329
XceI RCATGY 2 cut(s) 983, 2912
XcmI CCANNNNNNNNNTGG 3 cut(s) 1164, 2101, 3236
XhoI CTCGAG 1 cut(s) 245
XmiI GTMKAC 2 cut(s) 2324, 2946
XmnI GAANNNNTTC 3 cut(s) 215, 308, 2505
XspI CTAG 8 cut(s) 417, 431, 692, 995, 1145, 1680, 2963, 3068
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 2 cut(s) 1195, 2516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.