pycom13g14400

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007310
Physical Location & Seq
Forward (+)
10388010 .. 10398456
10447 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3405 bp
ATGAAACGGGTTGAGCCCCGTTTTGGCTGGTTTTATCCGCATTCCGCATGGTGTTTCCCTCTTTCTGCTGACACTTCAGTGGTAGTCACAGTCCCCAGAAATGGCGAAGAAGCTGCTCTTCCGATGAACTTCAAATCCTTCGCCGCCCACTCTTTGGCGCTCCAACCCAGGGAAGCCGCCATGGCTGCTATTCAAACCTCTCCCTACCGGGTTCTATCGCAAGGAACCTGCGCATCTTTTAGGAGCACAGCTTCCGTTGGCCTATTTTATTTCAGAGATAGGTCTTCAGTTAAAGTATTCTCCCTCCTTAACATGGTGCACTATTCTACCTTTTCCAATGACGAGTTTGGTTTGTCCTCAAAGCGAAGATCTCGAGGACCTGTTATGGCCGCAAAGAAAGCAGCTGAAGGAAAAAAGCAAGAAGACGGAAAATACAAGCACACAGTTGAGCTGCCAAAGACATCATTTAGCATGAGAGCAAACTCTTTAACGCGAGAGCCTGAAATCCAAAAGATATGGGATGACAATCAAGTGTTCAAAAGAGTTATTGGTAAAAATACAGGGGAAAATTTCATTCTTCACGATGGTCCTCCGTATGCCAATGGAGATCTTCACATAGGCCATGCTTTAAATAAGATTTTAAAGGATTTTATCAATCGTTACAAGCTTCTTCAAAATTATAAGGTTCATTATGTGCCTGGTTGGGATTGTCATGGCTTACCAATTGAGTTGAAAGTTCTGCAATCACTTGATCAAGCTGCTCGAAAGGATCTAACACCCATAAAGTTGAGAGCAAAGGCAGCTAAATTTGCCAAGCAAACCGTTAAAACTCAGATGGAATCATTTAAGCGTTATGGAGTGTGGGCAGACTGGAATAATCCTTACCTAACTCTTGATCCAGAATATGAAGCTGCTCAGATAGAAGTATTTGGCCAGATGGTCCTTCAAGGTTTCATCTACAGAGGCAGGAAACCAGTTCACTGGAGCCCCTCATCACGAACTGCTCTTGCAGAGGCTGAGTTGGAGTATCCAGAGGGGCATGTTTCAAGAAGCATGTACGCCATTTTCAAATTGGTCAGTGCCTCTCCAACTTCAGGTGGCTTATTAAATGAGTATTTCCCAGATGTGTGCTTGGCCATTTGGACAACAACTCCCTGGACTATTCCAGCCAATGCTGCGGTGGCAGTGAATGCCAAGCTTTTATATGCCATCGTTGAAGTACAGTCACTCCCAGAAGATTCTTTATCTGATGGAAACAAAAAGGGAAGGCCAGGAAATCTTTTGAAGGAAGAAAAGAAGCCTTTCCTTATTGTTGCTTCAGATCTTGTGCCAGCATTAGAATCAAAATGGGGTGTGAAGCTTGTTGTTAGGAAAAGGGTGCCAGGTTCAGATCTTGAAAACTGCAGGTATTTCCATCCGGTTTTTAATAGGGAATGCCCGGTTGTCATTGGAGGAGATTATATTACGACAGAATCAGGAACAGGACTGGTTCATACAGCTCCTGGACATGGCCAGGAGGATTATGTCACTGGCCAGAAGTACGGACTACCTATGCTTTCTCCTGTAGATGATGATGGAAAGTTCACTGAAGAAGCTGGAAAATTTTGTGGGCTTGATGTACTTGCGGATGGTAATATTGCTGTCGTGAAATACTTGGATGAACATTTATCAGTTATCATGGAAGAATCCTACCAGCATAAGTATCCATATGACTGGAGAACTAAAAAACCCACAATATTTAGGGCAACTGAGCAATGGTTTGCTTCAGTAGAAGGGTTTCGCGAGGCTGTTATGGATGCAATTGGTCATGTAAAATGGATTCCACCTAAGGCAGAAAACAGAATTTCTGCAATGACTTCTAGCCGCTCTGATTGGTGTATATCGCGGCAAAGGACATGGGGTGTTCCAATTCCAGTCTTTTATCATGTGGAGTCAAAGGAACCTCTGATGAATGAAGAGACTATTGACCATATCAAGTCGATAATATCCGAAAAGCGTAGTGATGCGTGGTGGTACATGAAGTTGGAGGATCTTCTCCCCGATAAATATCGTGATAAAGCATCTGAATATGAAAAGGGGACTGATACAATGGACGTATGGTTTGATTCAGGCTCTTCTTGGGCTGCAGTGTTGGGGAAAAGAAATAGCCATAGTCTTCCTGCAGATTTGTACCTTGAAGGTATGGATCAGCATCGTGGGTGGTTCCAGAGTTCTTTGCTAACAAGTGTTGCTACTAAAGGAAAGGCTCCATATTCCAGTGTTATAACACATGGATTTGTATTGGATGACAAGGGTTTGAAAATGAGCAAATCTTTGGGTAATGTAGTAGATCCACGGACTGTGATTGAAGGAGGGAAGAACCAAAAGGATGGCTATGGAGCAGATGTCCTGCGTCTCTGGGTTTCTAGTGTAGATTATACAGGCGATGTAACAATCGGCCCTCAAATTCTCCGTCAAATGTCAGACATTTATAGGAAGACGCGGGGAACATTGAGATACCTTTTGGGAAATCTTCATGATTGGAATGCTGATAGTGCTATCTCATACCACGATCTTCCCATGATTGATCAGTACGCACTGTTTCAGCTTGAAAATTTTGTGAAGATCAGTAAAGAGTGCTATGAAAACTATCAGTTCTTTAAGATATTTCAGATCATACAGCGGTTTGTTGTCGTTGACCTGTCAAACTTCTATTTTGATGTCGCCAAAGATCGCCTTTATGTTGGGGGCACAAGAAGTTTTACTAGGAGAAGTTGTCAAACAGTTCTTGCAGCGCTTCTCCTATCTGTAGTGAGAGTGATTGCCCCGATATTACCTCATTTGGCTGAAGATGTATGGCAAAACCTTCCGTTCCAGTACACTGACGAAGATGGCTCTGCTGCCGAATTTGTTTTTGAATCAAGATGGCCAGCTTTGAACAACACATGGCTCTCTCTTTCGAAAGAGGAAACTAATTTCTGGGAAAAAGTTCTTGAGTTGAGAACTGAGGTGAACAAAGTCCTGGAGGTTGCTCGTACGGAAAAGCTAATTGGCTCTAGTTTAGATGCCAAGGTCTACCTCCACACTTACGATTCCGGCCTGGCCTCTAGATTAGGTGAAATGTCTGCGGCCAACAACGACGCAGACACACTGCATCGCATATTCATAACATCTCAGGCAGAGGTTCTTCCTTCGTTGGAAAATGAGCTGATTGCGAACATACCTCACAAAGGAGAATACCTCATCGGAGAAAACGTCAGAGTCTGGATTGGAGTGGCTCGTGCCGAGGGCCTAAAGTGCGAAAGATGCTGGAATTATTCACCTCAAGTTGGTTCCTTTCCAGAGCACAGCACGCTTTGCGGCCGCTGCTATAATGTGGTAAATGTTCAACACTCTCCATCTCCGGCCGTAGCTGTGGTCAGTTGA

Protein Analysis

1135

Amino Acids

128.08

Weight (kDa)

7.02

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 169 - 812 4.4e-133 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 198 - 342 2.1e-12 tRNA synthetases class I (M)
Anticodon_1 PF08264 856 - 1019 2.1e-30 Anticodon-binding domain of tRNA ligase
zf-FPG_IleRS PF06827 1090 - 1119 3.6e-08 Zinc finger found in FPG and IleRS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 681, 2264
Acc16I TGCGCA 1 cut(s) 232
Acc36I ACCTGC 2 cut(s) 236, 1395
AccB1I GGYRCC 1 cut(s) 1378
AccB7I CCANNNNNTGG 1 cut(s) 154
AccBSI CCGCTC 1 cut(s) 1866
AccI GTMKAC 1 cut(s) 3054
AccII CGCG 4 cut(s) 493, 1782, 1885, 2482
AclWI GGATC 5 cut(s) 777, 890, 2037, 2193, 2324
AcoI YGGCCR 9 cut(s) 387, 931, 1134, 1510, 1531, 2906, 3108, 3340, 3384
AcsI RAATTY 7 cut(s) 568, 806, 1601, 1842, 2445, 2593, 2885
AcuI CTGAAG 8 cut(s) 60, 270, 426, 1077, 1302, 1608, 1749, 2847
AfaI GTAC 9 cut(s) 1058, 1221, 1541, 1620, 2015, 2171, 2573, 2858, 3016
AfeI AGCGCT 1 cut(s) 2775
AfiI CCNNNNNNNGG 9 cut(s) 23, 101, 154, 169, 313, 1094, 1508, 3209, 3308
AjnI CCWGG 9 cut(s) 167, 697, 1154, 1270, 1381, 1501, 1512, 3000, 3078
AjuI GAANNNNNNNTTGG 2 cut(s) 3012, 3044
AleI CACNNNNGTG 1 cut(s) 77
AloI GAACNNNNNNTCC 2 cut(s) 119, 151
Alw21I GWGCWC 3 cut(s) 248, 321, 3327
Alw26I GTCTC 2 cut(s) 1952, 2399
Alw44I GTGCAC 1 cut(s) 317
AlwI GGATC 5 cut(s) 777, 890, 2037, 2193, 2324
AlwNI CAGNNNCTG 3 cut(s) 1016, 1502, 3243
Ama87I CYCGRG 1 cut(s) 372
Aor51HI AGCGCT 1 cut(s) 2775
ApaLI GTGCAC 1 cut(s) 317
ApoI RAATTY 7 cut(s) 568, 806, 1601, 1842, 2445, 2593, 2885
ArsI GACNNNNNNTTYG 4 cut(s) 2084, 2116, 2437, 2469
Asp700I GAANNNNTTC 3 cut(s) 1301, 1776, 2967
AspLEI GCGC 3 cut(s) 160, 233, 2776
AspS9I GGNCC 5 cut(s) 377, 587, 940, 2438, 3268
AsuC2I CCSGG 2 cut(s) 209, 1439
AsuHPI GGTGA 3 cut(s) 3001, 3107, 3291
AsuII TTCGAA 1 cut(s) 2939
AvaI CYCGRG 1 cut(s) 372
AvaII GGWCC 3 cut(s) 377, 587, 940
AxyI CCTNAGG 1 cut(s) 1827
BaeGI GKGCMC 2 cut(s) 321, 2732
BalI TGGCCA 5 cut(s) 933, 1136, 1512, 1533, 2908
BanI GGYRCC 1 cut(s) 1378
BanII GRGCYC 2 cut(s) 18, 989
BauI CACGAG 1 cut(s) 3258
BbsI GAAGAC 4 cut(s) 276, 429, 2147, 2483
Bbv12I GWGCWC 3 cut(s) 248, 321, 3327
BceAI ACGGC 1 cut(s) 3371
BcgI CGANNNNNNTGC 2 cut(s) 95, 129
BciT130I CCWGG 9 cut(s) 169, 699, 1156, 1272, 1383, 1503, 1514, 3002, 3080
BciVI GTATCC 2 cut(s) 1038, 1713
BclI TGATCA 2 cut(s) 751, 2566
BcnI CCSGG 2 cut(s) 209, 1439
BcoDI GTCTC 2 cut(s) 1952, 2399
BfaI CTAG 5 cut(s) 1860, 2406, 2745, 3036, 3087
BfmI CTRYAG 6 cut(s) 958, 1402, 1563, 2124, 2160, 2787
BfoI RGCGCY 2 cut(s) 161, 2777
BfuAI ACCTGC 2 cut(s) 236, 1395
BfuI GTATCC 2 cut(s) 1038, 1713
BglI GCCNNNNNGGC 1 cut(s) 182
BglII AGATCT 4 cut(s) 368, 607, 1321, 1390
Bme18I GGWCC 3 cut(s) 377, 587, 940
BmeT110I CYCGRG 1 cut(s) 372
BmgT120I GGNCC 5 cut(s) 377, 587, 940, 2438, 3268
BmiI GGNNCC 7 cut(s) 226, 986, 1380, 1941, 2204, 2247, 3313
BmsI GCATC 8 cut(s) 242, 1786, 1993, 2069, 2200, 3034, 3142, 3275
BpiI GAAGAC 4 cut(s) 276, 429, 2147, 2483
BplI GAGNNNNNCTC 2 cut(s) 3204, 3236
BpmI CTGGAG 3 cut(s) 1003, 1735, 3023
Bpu14I TTCGAA 1 cut(s) 2939
BpuEI CTTGAG 2 cut(s) 2993, 3288
BpuMI CCSGG 2 cut(s) 209, 1439
BsaBI GATNNNNATC 3 cut(s) 525, 2046, 2190
BsaJI CCNNGG 7 cut(s) 167, 168, 180, 1154, 2333, 3048, 3264
BsaWI WCCGGW 1 cut(s) 1417
Bsc4I CCNNNNNNNGG 9 cut(s) 23, 101, 154, 169, 313, 1094, 1508, 3209, 3308
Bse1I ACTGG 9 cut(s) 875, 974, 986, 1491, 1534, 1718, 1913, 2256, 2854
Bse21I CCTNAGG 1 cut(s) 1827
Bse3DI GCAATG 2 cut(s) 1760, 1857
Bse8I GATNNNNATC 3 cut(s) 525, 2046, 2190
BseBI CCWGG 9 cut(s) 169, 699, 1156, 1272, 1383, 1503, 1514, 3002, 3080
BseDI CCNNGG 7 cut(s) 167, 168, 180, 1154, 2333, 3048, 3264
BseGI GGATG 7 cut(s) 526, 1414, 1633, 1663, 1801, 2290, 2374
BseJI GATNNNNATC 3 cut(s) 525, 2046, 2190
BseLI CCNNNNNNNGG 9 cut(s) 23, 101, 154, 169, 313, 1094, 1508, 3209, 3308
BseMI GCAATG 2 cut(s) 1760, 1857
BseMII CTCAG 6 cut(s) 845, 929, 1008, 1740, 2976, 3167
BseNI ACTGG 9 cut(s) 875, 974, 986, 1491, 1534, 1718, 1913, 2256, 2854
BseRI GAGGAG 1 cut(s) 1467
BseSI GKGCMC 2 cut(s) 321, 2732
BseX3I CGGCCG 2 cut(s) 3340, 3384
Bsh1236I CGCG 4 cut(s) 493, 1782, 1885, 2482
Bsh1285I CGRYCG 2 cut(s) 3343, 3387
BshNI GGYRCC 1 cut(s) 1378
BsiEI CGRYCG 2 cut(s) 3343, 3387
BsiHKAI GWGCWC 3 cut(s) 248, 321, 3327
BsiHKCI CYCGRG 1 cut(s) 372
BsiSI CCGG 5 cut(s) 208, 1418, 1439, 3075, 3383
BsiWI CGTACG 1 cut(s) 3014
BslFI GGGAC 2 cut(s) 77, 2092
BslI CCNNNNNNNGG 9 cut(s) 23, 101, 154, 169, 313, 1094, 1508, 3209, 3308
BsmAI GTCTC 2 cut(s) 1952, 2399
BsmBI CGTCTC 1 cut(s) 2399
BsmFI GGGAC 2 cut(s) 77, 2092
BsmI GAATGC 4 cut(s) 40, 1195, 1439, 2530
BsoBI CYCGRG 1 cut(s) 372
Bsp119I TTCGAA 1 cut(s) 2939
Bsp1286I GDGCHC 6 cut(s) 18, 248, 321, 989, 2732, 3327
Bsp19I CCATGG 1 cut(s) 180
Bsp68I TCGCGA 1 cut(s) 1782
BspCNI CTCAG 6 cut(s) 844, 928, 1009, 1741, 2977, 3166
BspFNI CGCG 4 cut(s) 493, 1782, 1885, 2482
BspHI TCATGA 1 cut(s) 2515
BspLI GGNNCC 7 cut(s) 226, 986, 1380, 1941, 2204, 2247, 3313
BspMAI CTGCAG 3 cut(s) 1406, 2128, 2164
BspMI ACCTGC 2 cut(s) 236, 1395
BspPI GGATC 5 cut(s) 777, 890, 2037, 2193, 2324
BspQI GCTCTTC 2 cut(s) 123, 2119
BspT104I TTCGAA 1 cut(s) 2939
BspT107I GGYRCC 1 cut(s) 1378
BsrBI CCGCTC 1 cut(s) 1866
BsrDI GCAATG 2 cut(s) 1760, 1857
BsrI ACTGG 9 cut(s) 875, 974, 986, 1491, 1534, 1718, 1913, 2256, 2854
BssECI CCNNGG 7 cut(s) 167, 168, 180, 1154, 2333, 3048, 3264
BssSI CACGAG 1 cut(s) 3258
BssT1I CCWWGG 2 cut(s) 180, 3048
Bst2BI CACGAG 1 cut(s) 3258
Bst2UI CCWGG 9 cut(s) 169, 699, 1156, 1272, 1383, 1503, 1514, 3002, 3080
Bst4CI ACNGT 7 cut(s) 91, 445, 823, 1224, 2341, 2580, 2764
Bst6I CTCTTC 3 cut(s) 123, 1950, 2119
BstAPI GCANNNNNTGC 2 cut(s) 1190, 3336
BstBI TTCGAA 1 cut(s) 2939
BstC8I GCNNGC 3 cut(s) 1332, 2910, 3332
BstDEI CTNAG 7 cut(s) 831, 915, 1017, 1749, 1827, 2985, 3153
BstDSI CCRYGG 2 cut(s) 180, 2333
BstENI CCTNNNNNAGG 1 cut(s) 3207
BstF5I GGATG 7 cut(s) 526, 1414, 1633, 1663, 1801, 2290, 2374
BstFNI CGCG 4 cut(s) 493, 1782, 1885, 2482
BstH2I RGCGCY 2 cut(s) 161, 2777
BstHHI GCGC 3 cut(s) 160, 233, 2776
BstMAI GTCTC 2 cut(s) 1952, 2399
BstMCI CGRYCG 2 cut(s) 3343, 3387
BstNI CCWGG 9 cut(s) 169, 699, 1156, 1272, 1383, 1503, 1514, 3002, 3080
BstNSI RCATGY 2 cut(s) 1043, 1057
BstSFI CTRYAG 6 cut(s) 958, 1402, 1563, 2124, 2160, 2787
BstSLI GKGCMC 2 cut(s) 321, 2732
BstUI CGCG 4 cut(s) 493, 1782, 1885, 2482
BstV2I GAAGAC 4 cut(s) 276, 429, 2147, 2483
BstX2I RGATCY 7 cut(s) 368, 607, 769, 1321, 1390, 2029, 2329
BstXI CCANNNNNNTGG 3 cut(s) 981, 1713, 2369
BstYI RGATCY 7 cut(s) 368, 607, 769, 1321, 1390, 2029, 2329
BstZI CGGCCG 2 cut(s) 3340, 3384
Bsu36I CCTNAGG 1 cut(s) 1827
BsuI GTATCC 2 cut(s) 1038, 1713
BtgI CCRYGG 2 cut(s) 180, 2333
BtgZI GCGATG 2 cut(s) 2439, 3119
BtsCI GGATG 7 cut(s) 526, 1414, 1633, 1663, 1801, 2290, 2374
BtsI GCAGTG 3 cut(s) 1191, 2133, 3128
BtuMI TCGCGA 1 cut(s) 1782
BveI ACCTGC 2 cut(s) 236, 1395
Cac8I GCNNGC 3 cut(s) 1332, 2910, 3332
CaiI CAGNNNCTG 3 cut(s) 1016, 1502, 3243
CciI TCATGA 1 cut(s) 2515
CciNI GCGGCCGC 1 cut(s) 3340
CfoI GCGC 3 cut(s) 160, 233, 2776
Cfr13I GGNCC 5 cut(s) 377, 587, 940, 2438, 3268
CseI GACGC 3 cut(s) 2381, 2488, 3128
Csp6I GTAC 9 cut(s) 1057, 1220, 1540, 1619, 2014, 2170, 2572, 2857, 3015
CviQI GTAC 9 cut(s) 1057, 1220, 1540, 1619, 2014, 2170, 2572, 2857, 3015
DdeI CTNAG 7 cut(s) 831, 915, 1017, 1749, 1827, 2985, 3153
DraI TTTAAA 2 cut(s) 630, 642
EaeI YGGCCR 9 cut(s) 387, 931, 1134, 1510, 1531, 2906, 3108, 3340, 3384
EagI CGGCCG 2 cut(s) 3340, 3384
Eam1104I CTCTTC 3 cut(s) 123, 1950, 2119
EarI CTCTTC 3 cut(s) 123, 1950, 2119
EclXI CGGCCG 2 cut(s) 3340, 3384
Eco130I CCWWGG 2 cut(s) 180, 3048
Eco24I GRGCYC 2 cut(s) 18, 989
Eco47I GGWCC 3 cut(s) 377, 587, 940
Eco47III AGCGCT 1 cut(s) 2775
Eco52I CGGCCG 2 cut(s) 3340, 3384
Eco57I CTGAAG 8 cut(s) 60, 270, 426, 1077, 1302, 1608, 1749, 2847
Eco81I CCTNAGG 1 cut(s) 1827
Eco88I CYCGRG 1 cut(s) 372
EcoNI CCTNNNNNAGG 1 cut(s) 3207
EcoO109I RGGNCCY 2 cut(s) 377, 3268
EcoRII CCWGG 9 cut(s) 167, 697, 1154, 1270, 1381, 1501, 1512, 3000, 3078
EcoT14I CCWWGG 2 cut(s) 180, 3048
EcoT38I GRGCYC 2 cut(s) 18, 989
ErhI CCWWGG 2 cut(s) 180, 3048
Esp3I CGTCTC 1 cut(s) 2399
FalI AAGNNNNNCTT 4 cut(s) 102, 134, 2700, 2732
FaqI GGGAC 2 cut(s) 77, 2092
FauI CCCGC 1 cut(s) 2475
FauNDI CATATG 1 cut(s) 1708
FbaI TGATCA 2 cut(s) 751, 2566
FblI GTMKAC 1 cut(s) 3054
FokI GGATG 7 cut(s) 533, 1401, 1640, 1670, 1808, 2297, 2381
FriOI GRGCYC 2 cut(s) 18, 989
FspBI CTAG 5 cut(s) 1860, 2406, 2745, 3036, 3087
FspI TGCGCA 1 cut(s) 232
GlaI GCGC 3 cut(s) 159, 232, 2775
GsuI CTGGAG 3 cut(s) 1003, 1735, 3023
HaeII RGCGCY 2 cut(s) 161, 2777
HapII CCGG 5 cut(s) 208, 1418, 1439, 3075, 3383
HgaI GACGC 3 cut(s) 2381, 2488, 3128
HhaI GCGC 3 cut(s) 160, 233, 2776
Hin6I GCGC 3 cut(s) 158, 231, 2774
HinP1I GCGC 3 cut(s) 158, 231, 2774
HincII GTYRAC 1 cut(s) 2677
HindII GTYRAC 1 cut(s) 2677
HindIII AAGCTT 3 cut(s) 665, 1196, 1358
HpaII CCGG 5 cut(s) 208, 1418, 1439, 3075, 3383
HphI GGTGA 3 cut(s) 3001, 3107, 3291
Hpy166II GTNNAC 7 cut(s) 319, 979, 1584, 2677, 2859, 2992, 3055
Hpy8I GTNNAC 7 cut(s) 319, 979, 1584, 2677, 2859, 2992, 3055
Hpy99I CGWCG 1 cut(s) 3122
HpyCH4III ACNGT 7 cut(s) 91, 445, 823, 1224, 2341, 2580, 2764
HpyCH4IV ACGT 2 cut(s) 2094, 3234
HpyF3I CTNAG 7 cut(s) 831, 915, 1017, 1749, 1827, 2985, 3153
HpySE526I ACGT 2 cut(s) 2094, 3234
HspAI GCGC 3 cut(s) 158, 231, 2774
Ksp22I TGATCA 2 cut(s) 751, 2566
LguI GCTCTTC 2 cut(s) 123, 2119
LmnI GCTCC 6 cut(s) 165, 243, 984, 1504, 2251, 2378
LweI GCATC 8 cut(s) 242, 1786, 1993, 2069, 2200, 3034, 3142, 3275
MaeI CTAG 5 cut(s) 1860, 2406, 2745, 3036, 3087
MaeII ACGT 2 cut(s) 2094, 3234
MaeIII GTNAC 5 cut(s) 85, 659, 1224, 1525, 2428
MbiI CCGCTC 1 cut(s) 1866
MfeI CAATTG 2 cut(s) 723, 1800
MflI RGATCY 7 cut(s) 368, 607, 769, 1321, 1390, 2029, 2329
MhlI GDGCHC 6 cut(s) 18, 248, 321, 989, 2732, 3327
MlsI TGGCCA 5 cut(s) 933, 1136, 1512, 1533, 2908
MluNI TGGCCA 5 cut(s) 933, 1136, 1512, 1533, 2908
MlyI GAGTC 2 cut(s) 1940, 3249
MmeI TCCRAC 5 cut(s) 187, 1002, 1112, 2004, 3156
Mox20I TGGCCA 5 cut(s) 933, 1136, 1512, 1533, 2908
MroXI GAANNNNTTC 3 cut(s) 1301, 1776, 2967
MscI TGGCCA 5 cut(s) 933, 1136, 1512, 1533, 2908
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 5 cut(s) 933, 1136, 1512, 1533, 2908
MspA1I CMGCKG 3 cut(s) 404, 2662, 3345
MspI CCGG 5 cut(s) 208, 1418, 1439, 3075, 3383
MunI CAATTG 2 cut(s) 723, 1800
Mva1269I GAATGC 4 cut(s) 40, 1195, 1439, 2530
MvaI CCWGG 9 cut(s) 169, 699, 1156, 1272, 1383, 1503, 1514, 3002, 3080
MvnI CGCG 4 cut(s) 493, 1782, 1885, 2482
NciI CCSGG 2 cut(s) 209, 1439
NcoI CCATGG 1 cut(s) 180
NdeI CATATG 1 cut(s) 1708
NlaIV GGNNCC 7 cut(s) 226, 986, 1380, 1941, 2204, 2247, 3313
NmeAIII GCCGAG 1 cut(s) 3289
NmuCI GTSAC 3 cut(s) 85, 1224, 1525
NotI GCGGCCGC 1 cut(s) 3340
NruI TCGCGA 1 cut(s) 1782
NsbI TGCGCA 1 cut(s) 232
NspI RCATGY 2 cut(s) 1043, 1057
NspV TTCGAA 1 cut(s) 2939
OliI CACNNNNGTG 1 cut(s) 77
PaeR7I CTCGAG 1 cut(s) 372
PagI TCATGA 1 cut(s) 2515
PasI CCCWGGG 1 cut(s) 168
PciSI GCTCTTC 2 cut(s) 123, 2119
PcsI WCGNNNNNNNCGW 1 cut(s) 3231
PctI GAATGC 4 cut(s) 40, 1195, 1439, 2530
PdmI GAANNNNTTC 3 cut(s) 1301, 1776, 2967
PfeI GAWTC 9 cut(s) 839, 1238, 1340, 1472, 1685, 1819, 2105, 2897, 3071
Pfl23II CGTACG 1 cut(s) 3014
PflMI CCANNNNNTGG 1 cut(s) 154
PfoI TCCNGGA 2 cut(s) 1501, 3000
PleI GAGTC 2 cut(s) 1939, 3248
PpsI GAGTC 2 cut(s) 1939, 3248
PpuMI RGGWCCY 1 cut(s) 377
PsiI TTATAA 2 cut(s) 681, 2264
Psp5II RGGWCCY 1 cut(s) 377
Psp6I CCWGG 9 cut(s) 167, 697, 1154, 1270, 1381, 1501, 1512, 3000, 3078
PspGI CCWGG 9 cut(s) 167, 697, 1154, 1270, 1381, 1501, 1512, 3000, 3078
PspLI CGTACG 1 cut(s) 3014
PspN4I GGNNCC 7 cut(s) 226, 986, 1380, 1941, 2204, 2247, 3313
PspPI GGNCC 5 cut(s) 377, 587, 940, 2438, 3268
PspPPI RGGWCCY 1 cut(s) 377
PstI CTGCAG 3 cut(s) 1406, 2128, 2164
PstNI CAGNNNCTG 3 cut(s) 1016, 1502, 3243
PsuI RGATCY 7 cut(s) 368, 607, 769, 1321, 1390, 2029, 2329
PvuII CAGCTG 1 cut(s) 404
RruI TCGCGA 1 cut(s) 1782
RsaI GTAC 9 cut(s) 1058, 1221, 1541, 1620, 2015, 2171, 2573, 2858, 3016
RsaNI GTAC 9 cut(s) 1057, 1220, 1540, 1619, 2014, 2170, 2572, 2857, 3015
RseI CAYNNNNRTG 1 cut(s) 77
SapI GCTCTTC 2 cut(s) 123, 2119
Sau96I GGNCC 5 cut(s) 377, 587, 940, 2438, 3268
SchI GAGTC 2 cut(s) 1940, 3249
SduI GDGCHC 6 cut(s) 18, 248, 321, 989, 2732, 3327
SfaNI GCATC 8 cut(s) 242, 1786, 1993, 2069, 2200, 3034, 3142, 3275
SfcI CTRYAG 6 cut(s) 958, 1402, 1563, 2124, 2160, 2787
Sfr274I CTCGAG 1 cut(s) 372
SfuI TTCGAA 1 cut(s) 2939
SinI GGWCC 3 cut(s) 377, 587, 940
SlaI CTCGAG 1 cut(s) 372
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 3 cut(s) 372, 2972, 3303
SmoI CTYRAG 3 cut(s) 372, 2972, 3303
SspI AATATT 2 cut(s) 1636, 1737
SspMI CTAG 5 cut(s) 1860, 2406, 2745, 3036, 3087
StyI CCWWGG 2 cut(s) 180, 3048
TaaI ACNGT 7 cut(s) 91, 445, 823, 1224, 2341, 2580, 2764
TaiI ACGT 2 cut(s) 2097, 3237
TaqI TCGA 4 cut(s) 373, 763, 1979, 2939
TatI WGTACW 3 cut(s) 1219, 1618, 2856
TauI GCSGC 8 cut(s) 146, 179, 392, 1866, 1888, 3110, 3342, 3345
TfiI GAWTC 9 cut(s) 839, 1238, 1340, 1472, 1685, 1819, 2105, 2897, 3071
TseFI GTSAC 3 cut(s) 85, 1224, 1525
Tsp45I GTSAC 3 cut(s) 85, 1224, 1525
TspGWI ACGGA 8 cut(s) 244, 441, 582, 1557, 2350, 2441, 2838, 3032
Van91I CCANNNNNTGG 1 cut(s) 154
VneI GTGCAC 1 cut(s) 317
VpaK11BI GGWCC 3 cut(s) 377, 587, 940
XagI CCTNNNNNAGG 1 cut(s) 3207
XapI RAATTY 7 cut(s) 568, 806, 1601, 1842, 2445, 2593, 2885
XbaI TCTAGA 1 cut(s) 3086
XceI RCATGY 2 cut(s) 1043, 1057
XcmI CCANNNNNNNNNTGG 2 cut(s) 1069, 1177
XhoI CTCGAG 1 cut(s) 372
XmiI GTMKAC 1 cut(s) 3054
XmnI GAANNNNTTC 3 cut(s) 1301, 1776, 2967
XspI CTAG 5 cut(s) 1860, 2406, 2745, 3036, 3087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.