pycom13g15870

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007097
Physical Location & Seq
Forward (+)
11618977 .. 11620933
1957 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1656 bp
ATGAGTAAAGAAGAGTTCTTGAAGATCCAGAAATGTGTTCTCAAAGTGAACATCCACTGTGATGGATGTAAACACAAAGTAAAGAAAATTCTGCAGAAAATCGATGGGGTTTTCACCACCGACATAGATTCAGAGCAGGGGAAGGTGACGGTCTCCGGAAATGTGGACCCTGCTATTCTGATTAAGAAGCTTGCAAAATCCGGCAAATATGCAGAGCTTTGGGGCGCTCCGAAGGCAAACAACAACCAGAGCAACCTCCCTAATCAGTTCAAGAACATGCAAATTGAAAATGGCAAGGGTGGAAACAATGGCGGTAAAGGCGGGCAGAATGGTGGTGGCGGCGGAGGCGGCGGTGCTAACAACCAGCCAAAGAGCGGCCAACAAGGCGGCCAGAAACCGCAGCAGCAGCAGCAGAACCAGCAGCTTCAGCAGCAGAACCAGCAGCTTCAGCAGCAGCAACTCAAGCAGCAGCAACTGCAGCAACTCCAGCTGCAGCAACTCCAGCAAATGAAGGGGTTCCAAGATGTGAAGCTGCCACAATTGAAGGGCATGCAAATGCCCCTTAGTTTCAAGGACCAGAACCCGAACCCGAACCCGAACCCGAACCAGAAGGCGGTGAAGTTCAACATGCCGGAGGAAGAGGATTTGAGTGACGATGAGTACGATGACCTTGATGACGATGATGACTATGACTATGACGACGATGAGTTTGAATATGACATGGATGAGCCCCATCATCCTCACACAAAGGGGAAGCCTGTGGTGATGGGCAATGGCGGCCAGGGGATGCCTCCGAACATGGGGATGATGAATGAGATGATGAAGGGGAACCATCCTCAGATGATGAATGCCGCCGCCCAAAAGGGTGTTGGCGGGAATGGTGGTGCCCAGAATGGAGGTGGTGCTGTGGTTAATGGGAAGAAGGGAGGAGGAGGTGGTGGTGGGCCTGTGCCTGTTGGTGGTGGGAACAATGAGGGTAAAAATGGAAGTGCAGGAAAGAAGGGCGGTGCCGGTGGTGGAGACCAACACCACAATCAAGGAGGCGGGGGTGGAAAGAATGGAGGCAAAAATGGTGGTGGGGGAAATGCCAAGAATGGAAACAGTGGTCAAGGGGGTCCTAATGGGAGCATGAACAATGGCAATGGGGGTAAAAAAGTTGGTGGTGGCGGCGGAGGAGGAATGAGTGAAGTTCAAGCTATGAACAATGCTTTTCAGAACATGGGTGGGCGTCCTCAGGGCATGCCTGGAGTTAGTGTGGGTCAGATGGGCAACATGAACATGGGAATGGGGCCAATGGGCAGCATGCCTATGAGCCAAATGGGCAACATTCCAGCTGTTCAAGGCCTTCCGGCGGGTGCCATGAATGGCGGTGGTGGAGGCGCCGGCGGAGGGTACTTCCAAGGAGCTGGGCCTGAGGCCATGCCCGGTAACCCCTACCAACAACAACAATACTTGCAGGCTGTGATGAACCAGCAAAGGGCAATGGGCAATGAGAGGTTCCAACCAATGATGTATGCTAGGCCACCACCTGCTGTCAACTACATGCCCCCCGGCCCACCGGCGCCATACCCATACCCATACGCTCCTCCTCCAGCTGGTGAACCATACACTCACTTCTTCAGTGATGAGAACACCTCAAGTTGCAATGTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

552

Amino Acids

57.4

Weight (kDa)

7.64

Isoelectric Point (pI)

50.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 14 - 67 6.9e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1537
Acc36I ACCTGC 1 cut(s) 1537
AccB1I GGYRCC 5 cut(s) 884, 1007, 1355, 1379, 1561
AccBSI CCGCTC 1 cut(s) 375
AccIII TCCGGA 1 cut(s) 155
AclWI GGATC 1 cut(s) 19
AcoI YGGCCR 3 cut(s) 376, 388, 778
AcsI RAATTY 1 cut(s) 87
AcuI CTGAAG 3 cut(s) 410, 431, 1603
AcyI GRCGYC 3 cut(s) 1228, 1380, 1562
AfaI GTAC 2 cut(s) 662, 1394
AfiI CCNNNNNNNGG 8 cut(s) 374, 613, 800, 959, 1351, 1389, 1477, 1595
AgsI TTSAA 9 cut(s) 22, 271, 287, 544, 571, 625, 713, 1193, 1340
AjnI CCWGG 2 cut(s) 780, 1243
Alw26I GTCTC 2 cut(s) 157, 1014
AlwI GGATC 1 cut(s) 19
AlwNI CAGNNNCTG 1 cut(s) 475
Aor13HI TCCGGA 1 cut(s) 155
ApoI RAATTY 1 cut(s) 87
ArsI GACNNNNNNTTYG 2 cut(s) 692, 724
Asp700I GAANNNNTTC 1 cut(s) 515
AspLEI GCGC 3 cut(s) 227, 1382, 1564
AspS9I GGNCC 7 cut(s) 166, 574, 944, 1115, 1289, 1409, 1553
AsuC2I CCSGG 2 cut(s) 1425, 1551
AsuHPI GGTGA 5 cut(s) 106, 157, 628, 775, 1610
AvaII GGWCC 3 cut(s) 166, 574, 1115
AxyI CCTNAGG 2 cut(s) 1233, 1413
BaeGI GKGCMC 1 cut(s) 889
BanI GGYRCC 5 cut(s) 884, 1007, 1355, 1379, 1561
BanII GRGCYC 1 cut(s) 732
BccI CCATC 6 cut(s) 56, 98, 741, 760, 840, 1258
BciT130I CCWGG 2 cut(s) 782, 1245
BcnI CCSGG 2 cut(s) 1425, 1551
BcoDI GTCTC 2 cut(s) 157, 1014
BfaI CTAG 1 cut(s) 1518
BfmI CTRYAG 3 cut(s) 92, 476, 491
BfoI RGCGCY 3 cut(s) 228, 1383, 1565
BfuAI ACCTGC 1 cut(s) 1537
BglI GCCNNNNNGGC 2 cut(s) 384, 1320
Bme1390I CCNGG 4 cut(s) 782, 1245, 1425, 1551
Bme18I GGWCC 3 cut(s) 166, 574, 1115
BmgT120I GGNCC 7 cut(s) 166, 574, 944, 1115, 1289, 1409, 1553
BmrFI CCNGG 4 cut(s) 782, 1245, 1425, 1551
BmsI GCATC 1 cut(s) 777
BplI GAGNNNNNCTC 2 cut(s) 1619, 1651
BpmI CTGGAG 4 cut(s) 470, 485, 1266, 1575
BpuEI CTTGAG 2 cut(s) 446, 1621
BpuMI CCSGG 2 cut(s) 1425, 1551
Bsa29I ATCGAT 1 cut(s) 102
BsaHI GRCGYC 3 cut(s) 1228, 1380, 1562
BsaI GGTCTC 2 cut(s) 157, 1014
BsaJI CCNNGG 3 cut(s) 781, 1399, 1549
BsaWI WCCGGW 1 cut(s) 155
BsaXI ACNNNNNCTCC 2 cut(s) 1239, 1269
Bsc4I CCNNNNNNNGG 8 cut(s) 374, 613, 800, 959, 1351, 1389, 1477, 1595
Bse118I RCCGGY 3 cut(s) 1010, 1382, 1558
Bse21I CCTNAGG 2 cut(s) 1233, 1413
Bse3DI GCAATG 5 cut(s) 778, 1147, 1488, 1495, 1651
BseAI TCCGGA 1 cut(s) 155
BseBI CCWGG 2 cut(s) 782, 1245
BseCI ATCGAT 1 cut(s) 102
BseDI CCNNGG 3 cut(s) 781, 1399, 1549
BseGI GGATG 7 cut(s) 51, 71, 730, 736, 792, 810, 832
BseLI CCNNNNNNNGG 8 cut(s) 374, 613, 800, 959, 1351, 1389, 1477, 1595
BseMI GCAATG 5 cut(s) 778, 1147, 1488, 1495, 1651
BseMII CTCAG 3 cut(s) 851, 1247, 1404
BseRI GAGGAG 5 cut(s) 942, 945, 1188, 1575, 1578
BseSI GKGCMC 1 cut(s) 889
BseYI CCCAGC 1 cut(s) 1406
BsgI GTGCAG 1 cut(s) 1011
BshNI GGYRCC 5 cut(s) 884, 1007, 1355, 1379, 1561
BshVI ATCGAT 1 cut(s) 102
BsiSI CCGG 9 cut(s) 156, 201, 632, 1011, 1349, 1383, 1425, 1551, 1559
BslI CCNNNNNNNGG 8 cut(s) 374, 613, 800, 959, 1351, 1389, 1477, 1595
BsmAI GTCTC 2 cut(s) 157, 1014
BsmI GAATGC 1 cut(s) 853
Bso31I GGTCTC 2 cut(s) 157, 1014
Bsp1286I GDGCHC 2 cut(s) 732, 889
Bsp13I TCCGGA 1 cut(s) 155
Bsp143I GATC 1 cut(s) 24
BspCNI CTCAG 3 cut(s) 850, 1246, 1405
BspDI ATCGAT 1 cut(s) 102
BspEI TCCGGA 1 cut(s) 155
BspMAI CTGCAG 3 cut(s) 96, 480, 495
BspMI ACCTGC 1 cut(s) 1537
BspPI GGATC 1 cut(s) 19
BspT107I GGYRCC 5 cut(s) 884, 1007, 1355, 1379, 1561
BspTNI GGTCTC 2 cut(s) 157, 1014
BsrBI CCGCTC 1 cut(s) 375
BsrDI GCAATG 5 cut(s) 778, 1147, 1488, 1495, 1651
BsrFI RCCGGY 3 cut(s) 1010, 1382, 1558
BssAI RCCGGY 3 cut(s) 1010, 1382, 1558
BssECI CCNNGG 3 cut(s) 781, 1399, 1549
BssMI GATC 1 cut(s) 24
BssNI GRCGYC 3 cut(s) 1228, 1380, 1562
BssT1I CCWWGG 1 cut(s) 1399
Bst2UI CCWGG 2 cut(s) 782, 1245
Bst4CI ACNGT 3 cut(s) 59, 151, 1103
Bst6I CTCTTC 2 cut(s) 6, 633
BstACI GRCGYC 3 cut(s) 1228, 1380, 1562
BstAPI GCANNNNNTGC 1 cut(s) 475
BstC8I GCNNGC 7 cut(s) 192, 323, 551, 1241, 1304, 1384, 1458
BstDEI CTNAG 4 cut(s) 563, 837, 1233, 1413
BstEII GGTNACC 1 cut(s) 1427
BstF5I GGATG 7 cut(s) 51, 71, 730, 736, 792, 810, 832
BstH2I RGCGCY 3 cut(s) 228, 1383, 1565
BstHHI GCGC 3 cut(s) 227, 1382, 1564
BstKTI GATC 1 cut(s) 27
BstMAI GTCTC 2 cut(s) 157, 1014
BstMBI GATC 1 cut(s) 24
BstNI CCWGG 2 cut(s) 782, 1245
BstNSI RCATGY 6 cut(s) 280, 553, 631, 1243, 1306, 1546
BstPI GGTNACC 1 cut(s) 1427
BstSCI CCNGG 4 cut(s) 780, 1243, 1423, 1549
BstSFI CTRYAG 3 cut(s) 92, 476, 491
BstSLI GKGCMC 1 cut(s) 889
BstX2I RGATCY 1 cut(s) 24
BstXI CCANNNNNNTGG 2 cut(s) 62, 1406
BstYI RGATCY 1 cut(s) 24
Bsu15I ATCGAT 1 cut(s) 102
Bsu36I CCTNAGG 2 cut(s) 1233, 1413
BsuTUI ATCGAT 1 cut(s) 102
BtsCI GGATG 7 cut(s) 51, 71, 730, 736, 792, 810, 832
BtsIMutI CAGTG 3 cut(s) 55, 1108, 1627
BveI ACCTGC 1 cut(s) 1537
Cac8I GCNNGC 7 cut(s) 192, 323, 551, 1241, 1304, 1384, 1458
CaiI CAGNNNCTG 1 cut(s) 475
CfoI GCGC 3 cut(s) 227, 1382, 1564
Cfr10I RCCGGY 3 cut(s) 1010, 1382, 1558
Cfr13I GGNCC 7 cut(s) 166, 574, 944, 1115, 1289, 1409, 1553
ClaI ATCGAT 1 cut(s) 102
CseI GACGC 1 cut(s) 1217
Csp6I GTAC 2 cut(s) 661, 1393
CspCI CAANNNNNGTGG 2 cut(s) 1054, 1089
CviQI GTAC 2 cut(s) 661, 1393
DdeI CTNAG 4 cut(s) 563, 837, 1233, 1413
DinI GGCGCC 2 cut(s) 1381, 1563
DpnI GATC 1 cut(s) 26
DpnII GATC 1 cut(s) 24
EaeI YGGCCR 3 cut(s) 376, 388, 778
Eam1104I CTCTTC 2 cut(s) 6, 633
EarI CTCTTC 2 cut(s) 6, 633
EciI GGCGGA 3 cut(s) 357, 1185, 1401
Eco130I CCWWGG 1 cut(s) 1399
Eco147I AGGCCT 1 cut(s) 1344
Eco24I GRGCYC 1 cut(s) 732
Eco31I GGTCTC 2 cut(s) 157, 1014
Eco47I GGWCC 3 cut(s) 166, 574, 1115
Eco57I CTGAAG 3 cut(s) 410, 431, 1603
Eco81I CCTNAGG 2 cut(s) 1233, 1413
Eco91I GGTNACC 1 cut(s) 1427
EcoO109I RGGNCCY 1 cut(s) 1115
EcoO65I GGTNACC 1 cut(s) 1427
EcoRII CCWGG 2 cut(s) 780, 1243
EcoT14I CCWWGG 1 cut(s) 1399
EcoT38I GRGCYC 1 cut(s) 732
EgeI GGCGCC 2 cut(s) 1381, 1563
EheI GGCGCC 2 cut(s) 1381, 1563
ErhI CCWWGG 1 cut(s) 1399
FauI CCCGC 4 cut(s) 314, 866, 1037, 1345
FokI GGATG 7 cut(s) 38, 78, 723, 737, 799, 817, 819
FriOI GRGCYC 1 cut(s) 732
FspBI CTAG 1 cut(s) 1518
GlaI GCGC 3 cut(s) 226, 1381, 1563
GsaI CCCAGC 1 cut(s) 1410
GsuI CTGGAG 4 cut(s) 470, 485, 1266, 1575
HaeII RGCGCY 3 cut(s) 228, 1383, 1565
HapII CCGG 9 cut(s) 156, 201, 632, 1011, 1349, 1383, 1425, 1551, 1559
HgaI GACGC 1 cut(s) 1217
HhaI GCGC 3 cut(s) 227, 1382, 1564
Hin1I GRCGYC 3 cut(s) 1228, 1380, 1562
Hin6I GCGC 3 cut(s) 225, 1380, 1562
HinP1I GCGC 3 cut(s) 225, 1380, 1562
HincII GTYRAC 1 cut(s) 1537
HindII GTYRAC 1 cut(s) 1537
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 1 cut(s) 128
HpaII CCGG 9 cut(s) 156, 201, 632, 1011, 1349, 1383, 1425, 1551, 1559
HphI GGTGA 5 cut(s) 106, 157, 628, 775, 1610
Hpy166II GTNNAC 5 cut(s) 49, 71, 166, 1537, 1601
Hpy188I TCNGA 7 cut(s) 133, 180, 231, 795, 840, 1215, 1263
Hpy188III TCNNGA 4 cut(s) 19, 28, 156, 271
Hpy8I GTNNAC 5 cut(s) 49, 71, 166, 1537, 1601
Hpy99I CGWCG 1 cut(s) 704
HpyAV CCTTC 9 cut(s) 136, 226, 505, 538, 604, 817, 916, 994, 1355
HpyCH4III ACNGT 3 cut(s) 59, 151, 1103
HpyF3I CTNAG 4 cut(s) 563, 837, 1233, 1413
Hsp92I GRCGYC 3 cut(s) 1228, 1380, 1562
HspAI GCGC 3 cut(s) 225, 1380, 1562
KasI GGCGCC 2 cut(s) 1379, 1561
Kpn2I TCCGGA 1 cut(s) 155
KroI GCCGGC 1 cut(s) 1382
KroNI GCCGGC 1 cut(s) 1384
Kzo9I GATC 1 cut(s) 24
LmnI GCTCC 4 cut(s) 232, 1125, 1403, 1588
LweI GCATC 1 cut(s) 777
MaeI CTAG 1 cut(s) 1518
MaeIII GTNAC 3 cut(s) 145, 650, 1427
MalI GATC 1 cut(s) 26
MbiI CCGCTC 1 cut(s) 375
MboI GATC 1 cut(s) 24
MboII GAAGA 5 cut(s) 23, 34, 650, 931, 1609
MfeI CAATTG 1 cut(s) 539
MflI RGATCY 1 cut(s) 24
MhlI GDGCHC 2 cut(s) 732, 889
MluCI AATT 3 cut(s) 87, 282, 539
Mly113I GGCGCC 2 cut(s) 1380, 1562
MmeI TCCRAC 1 cut(s) 1525
MreI CGCCGGCG 1 cut(s) 1382
MroI TCCGGA 1 cut(s) 155
MroNI GCCGGC 1 cut(s) 1382
MroXI GAANNNNTTC 1 cut(s) 515
MseI TTAA 2 cut(s) 183, 912
MslI CAYNNNNRTG 6 cut(s) 60, 554, 803, 1277, 1283, 1307
MspA1I CMGCKG 3 cut(s) 490, 1334, 1595
MspI CCGG 9 cut(s) 156, 201, 632, 1011, 1349, 1383, 1425, 1551, 1559
MspR9I CCNGG 4 cut(s) 782, 1245, 1425, 1551
MunI CAATTG 1 cut(s) 539
Mva1269I GAATGC 1 cut(s) 853
MvaI CCWGG 2 cut(s) 782, 1245
NaeI GCCGGC 1 cut(s) 1384
NarI GGCGCC 2 cut(s) 1380, 1562
NciI CCSGG 2 cut(s) 1425, 1551
NdeII GATC 1 cut(s) 24
NgoMIV GCCGGC 1 cut(s) 1382
NmuCI GTSAC 2 cut(s) 145, 650
NspI RCATGY 6 cut(s) 280, 553, 631, 1243, 1306, 1546
PaeI GCATGC 3 cut(s) 553, 1243, 1306
PaqCI CACCTGC 1 cut(s) 1537
PceI AGGCCT 1 cut(s) 1344
PcsI WCGNNNNNNNCGW 1 cut(s) 660
PctI GAATGC 1 cut(s) 853
PdiI GCCGGC 1 cut(s) 1384
PdmI GAANNNNTTC 1 cut(s) 515
PfeI GAWTC 1 cut(s) 128
PluTI GGCGCC 2 cut(s) 1383, 1565
PpuMI RGGWCCY 1 cut(s) 1115
Psp5II RGGWCCY 1 cut(s) 1115
Psp6I CCWGG 2 cut(s) 780, 1243
PspEI GGTNACC 1 cut(s) 1427
PspFI CCCAGC 1 cut(s) 1406
PspGI CCWGG 2 cut(s) 780, 1243
PspPI GGNCC 7 cut(s) 166, 574, 944, 1115, 1289, 1409, 1553
PspPPI RGGWCCY 1 cut(s) 1115
PstI CTGCAG 3 cut(s) 96, 480, 495
PstNI CAGNNNCTG 1 cut(s) 475
PsuI RGATCY 1 cut(s) 24
PvuII CAGCTG 3 cut(s) 490, 1334, 1595
RsaI GTAC 2 cut(s) 662, 1394
RsaNI GTAC 2 cut(s) 661, 1393
RseI CAYNNNNRTG 6 cut(s) 60, 554, 803, 1277, 1283, 1307
SaqAI TTAA 2 cut(s) 183, 912
Sau3AI GATC 1 cut(s) 24
Sau96I GGNCC 7 cut(s) 166, 574, 944, 1115, 1289, 1409, 1553
ScrFI CCNGG 4 cut(s) 782, 1245, 1425, 1551
SduI GDGCHC 2 cut(s) 732, 889
SfaNI GCATC 1 cut(s) 777
SfcI CTRYAG 3 cut(s) 92, 476, 491
SfoI GGCGCC 2 cut(s) 1381, 1563
SgrAI CRCCGGYG 2 cut(s) 1382, 1558
SinI GGWCC 3 cut(s) 166, 574, 1115
SmiMI CAYNNNNRTG 6 cut(s) 60, 554, 803, 1277, 1283, 1307
SmlI CTYRAG 2 cut(s) 461, 1636
SmoI CTYRAG 2 cut(s) 461, 1636
SphI GCATGC 3 cut(s) 553, 1243, 1306
Sse9I AATT 3 cut(s) 87, 282, 539
SseBI AGGCCT 1 cut(s) 1344
SspDI GGCGCC 2 cut(s) 1379, 1561
SspMI CTAG 1 cut(s) 1518
StuI AGGCCT 1 cut(s) 1344
StyD4I CCNGG 4 cut(s) 780, 1243, 1423, 1549
StyI CCWWGG 1 cut(s) 1399
TaaI ACNGT 3 cut(s) 59, 151, 1103
TaqI TCGA 1 cut(s) 102
TasI AATT 3 cut(s) 87, 282, 539
TauI GCSGC 8 cut(s) 342, 351, 378, 390, 780, 854, 857, 1170
TfiI GAWTC 1 cut(s) 128
Tru1I TTAA 2 cut(s) 183, 912
Tru9I TTAA 2 cut(s) 183, 912
TscAI CASTG 3 cut(s) 62, 1108, 1627
TseFI GTSAC 2 cut(s) 145, 650
Tsp45I GTSAC 2 cut(s) 145, 650
TspDTI ATGAA 9 cut(s) 524, 824, 836, 860, 1145, 1214, 1289, 1376, 1481
TspRI CASTG 3 cut(s) 62, 1108, 1627
VpaK11BI GGWCC 3 cut(s) 166, 574, 1115
XapI RAATTY 1 cut(s) 87
XceI RCATGY 6 cut(s) 280, 553, 631, 1243, 1306, 1546
XcmI CCANNNNNNNNNTGG 2 cut(s) 866, 896
XmnI GAANNNNTTC 1 cut(s) 515
XspI CTAG 1 cut(s) 1518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.