Rroxscaffold_5G00361490

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0007097
Physical Location & Seq
Reverse (-)
42198593 .. 42201474
2882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00361490.1

Sequence Viewer

Length: 1635 bp
ATGAGTAAAGAAGAGTTCTTGAAGATCCAGAAATGTGCTCTCAAAGTTAACATTCACTGTGATGGATGCAAGCACAAGGTGAAGAAAATCTTGCAGAGAATTGATGGGGTTTTTACAACCGACATAGATTCCGAGCAGGGGAAGGTGGTCGTTTCCGGCAATGTTGACCCCTCCGTTCTGATCAAGAAGCTCGCGAAATCAGGGAAACATGCAGAGCTCTGGGGCGCTCCGAAGGCCAACAACAACAATCAGAACCAGAACAACATGGCCAATCAGTCCAAGAACATGCAACTTGACAATGGCAAAGGTGGGAATAACAAAGGCCAGAACAACCAGAAGGGCAGCGGTGGAGGCGGAGGAGGAGGAGGCAACAACAATCAGCCTAAGGGTGCAGGACAACAAGGCCCAAACCCTCAGCAACAGCTTCAAATTCAACAGCAACAGCAAATGCAGCAGCAACTCCAGCAGCTGCAGCAGCTTCAGCAAATGAAGGGGTTGCAAGATCTGAAGCTGCCCCAATTGAAAGCGATGCAAATGCCTCCTGGTTTTAAAGGGCCGAACCCACCTAGCCAGAACCAGAAGGCTGTCAAATTCGACGTGGACGAAGATGACATGACTGATGAGGAGTATGATGATCTTGATGATGACGAAGATTATGACGACGAGGATTTTGATGACGAGCTGGATGATCCTCGCCTGGCTCTGAATAAGATGAAGCCGGCGACAATGGGTGGTGGTGGTGGTGGTCAGGGGCTGCCTCCTATGATGATGAATGGAATGAACCACCAACAGATGATGAATGTTCAGAAGGCTGGTGGTGGACAACAGAATGGTGGTGGTGGCGGCGCTGGTAATGGTGGAAAGAAAGGTGGTGGCGGCGGCGGCGGAGGAGGAGGGGGTGCGCCGTCTCAGGGGAATATGGGTGGTGGTGGAGGCGGTGGAAAGAAAGGCGGTGGCGGTGGTGTAGATCATGGTCAAAATCAAGCTGGGGGTGGCGGCAAAAATGGAGGCAAGAACAGTGGTGGGTTTCCCCCAAATGGTGGTGGTGGGGCACAGAACAAGAATGGTAACGGAGGCCAAGGTGGTGGTGCTATTGGCAATGGAGCTAAAAAGGGTGGTGGTGGTGGTGGTGGGATGAGTGAGGCTCAACTTCATGCTATGATGAGCCACCACAACATGGGTGGGAGACCTCAAGGCATGCCTGGAGGTAATGTGGGGCAGCAGATGCCTGGGATGAGCCAGATGAACATGGCAATGGGTCCTATGGGTCCAATGGGTCCAATGGGAAGCTTGCCAATGGGTCAGATGGGCCAAATGCCGGCTGTTCAAGGTCTACCGGCTGCCGCCATGAGTGGCGGCGGCGGCGGCGGCCCCGGTGGCTACTTCCAAGGAGCTGGGCCTGAGGCCATGCCTGGTAACCCATACCAGAACCCACAGTTAATAGCTGCAATGATGAACCAGCAGAGAGCTATGGGCGCCGGGGACAGGTCGTTCCAGCCTATGATGTATAATAGGGTTTCTCCATCGACCAACTACATGCCGCCCCAGCATTACCCATTCCCTCCTCCAGCTGGTGGTGAACCCTACACCCATTTCTTCAGTGATGAGAACACTTCAAGCTGCAATGTGATGTGA

Protein Analysis

544

Amino Acids

55.56

Weight (kDa)

7.67

Isoelectric Point (pI)

56.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 15 - 70 1.5e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1475
AccB7I CCANNNNNTGG 3 cut(s) 1040, 1177, 1574
AccI GTMKAC 1 cut(s) 1333
AccII CGCG 1 cut(s) 194
AclWI GGATC 2 cut(s) 19, 683
AcoI YGGCCR 1 cut(s) 267
AcsI RAATTY 2 cut(s) 429, 590
AcuI CTGAAG 3 cut(s) 464, 527, 1582
AcyI GRCGYC 1 cut(s) 1476
AdeI CACNNNGTG 1 cut(s) 79
AfiI CCNNNNNNNGG 9 cut(s) 138, 911, 1037, 1040, 1177, 1318, 1485, 1571, 1574
AgsI TTSAA 6 cut(s) 22, 428, 434, 523, 1328, 1617
AjiI CACGTC 1 cut(s) 598
AjnI CCWGG 5 cut(s) 541, 696, 1201, 1228, 1411
AloI GAACNNNNNNTCC 2 cut(s) 1475, 1507
Alw21I GWGCWC 2 cut(s) 40, 219
Alw26I GTCTC 2 cut(s) 912, 1180
AlwI GGATC 2 cut(s) 19, 683
AlwNI CAGNNNCTG 2 cut(s) 469, 754
ApoI RAATTY 2 cut(s) 429, 590
ArsI GACNNNNNNTTYG 2 cut(s) 653, 685
AspLEI GCGC 4 cut(s) 227, 848, 904, 1478
AspS9I GGNCC 8 cut(s) 404, 554, 1259, 1268, 1277, 1309, 1370, 1397
AsuC2I CCSGG 2 cut(s) 1374, 1480
AsuHPI GGTGA 2 cut(s) 91, 1589
AvaII GGWCC 3 cut(s) 1259, 1268, 1277
AxyI CCTNAGG 2 cut(s) 384, 1401
BaeGI GKGCMC 1 cut(s) 1054
BalI TGGCCA 1 cut(s) 269
BanI GGYRCC 1 cut(s) 1475
BanII GRGCYC 1 cut(s) 219
Bbv12I GWGCWC 2 cut(s) 40, 219
BbvCI CCTCAGC 1 cut(s) 414
BccI CCATC 4 cut(s) 56, 98, 1300, 1531
BceAI ACGGC 1 cut(s) 889
BcgI CGANNNNNNTGC 2 cut(s) 517, 551
BciT130I CCWGG 5 cut(s) 543, 698, 1203, 1230, 1413
BclI TGATCA 1 cut(s) 180
BcnI CCSGG 2 cut(s) 1374, 1480
BcoDI GTCTC 2 cut(s) 912, 1180
BfaI CTAG 1 cut(s) 567
BfmI CTRYAG 1 cut(s) 470
BfoI RGCGCY 3 cut(s) 228, 849, 1479
BglI GCCNNNNNGGC 1 cut(s) 1377
BglII AGATCT 1 cut(s) 502
Bme1390I CCNGG 7 cut(s) 543, 698, 1203, 1230, 1374, 1413, 1480
Bme18I GGWCC 3 cut(s) 1259, 1268, 1277
BmgBI CACGTC 1 cut(s) 598
BmgT120I GGNCC 8 cut(s) 404, 554, 1259, 1268, 1277, 1309, 1370, 1397
BmiI GGNNCC 5 cut(s) 1260, 1269, 1278, 1372, 1477
BmrFI CCNGG 7 cut(s) 543, 698, 1203, 1230, 1374, 1413, 1480
BmsI GCATC 3 cut(s) 56, 519, 1215
BplI GAGNNNNNCTC 2 cut(s) 1129, 1161
BpmI CTGGAG 3 cut(s) 446, 1224, 1551
Bpu10I CCTNAGC 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 1176
BpuMI CCSGG 2 cut(s) 1374, 1480
BsaHI GRCGYC 1 cut(s) 1476
BsaI GGTCTC 1 cut(s) 1180
BsaJI CCNNGG 5 cut(s) 1078, 1229, 1372, 1387, 1479
BsaXI ACNNNNNCTCC 2 cut(s) 1197, 1227
Bsc4I CCNNNNNNNGG 9 cut(s) 138, 911, 1037, 1040, 1177, 1318, 1485, 1571, 1574
Bse118I RCCGGY 3 cut(s) 718, 1318, 1336
Bse21I CCTNAGG 2 cut(s) 384, 1401
Bse3DI GCAATG 5 cut(s) 166, 1105, 1260, 1455, 1630
BseBI CCWGG 5 cut(s) 543, 698, 1203, 1230, 1413
BseDI CCNNGG 5 cut(s) 1078, 1229, 1372, 1387, 1479
BseGI GGATG 4 cut(s) 71, 691, 1140, 1239
BseLI CCNNNNNNNGG 9 cut(s) 138, 911, 1037, 1040, 1177, 1318, 1485, 1571, 1574
BseMI GCAATG 5 cut(s) 166, 1105, 1260, 1455, 1630
BseMII CTCAG 3 cut(s) 428, 923, 1392
BseRI GAGGAG 7 cut(s) 372, 375, 378, 638, 903, 906, 1554
BseSI GKGCMC 1 cut(s) 1054
BseYI CCCAGC 3 cut(s) 986, 1394, 1545
BsgI GTGCAG 1 cut(s) 411
Bsh1236I CGCG 1 cut(s) 194
BshNI GGYRCC 1 cut(s) 1475
BsiHKAI GWGCWC 2 cut(s) 40, 219
BsiSI CCGG 6 cut(s) 156, 719, 1319, 1337, 1374, 1479
BslFI GGGAC 1 cut(s) 1496
BslI CCNNNNNNNGG 9 cut(s) 138, 911, 1037, 1040, 1177, 1318, 1485, 1571, 1574
BsmAI GTCTC 2 cut(s) 912, 1180
BsmBI CGTCTC 1 cut(s) 912
BsmFI GGGAC 1 cut(s) 1496
Bso31I GGTCTC 1 cut(s) 1180
Bsp1286I GDGCHC 3 cut(s) 40, 219, 1054
Bsp143I GATC 6 cut(s) 24, 180, 502, 634, 688, 967
Bsp68I TCGCGA 1 cut(s) 194
BspCNI CTCAG 3 cut(s) 427, 922, 1393
BspFNI CGCG 1 cut(s) 194
BspLI GGNNCC 5 cut(s) 1260, 1269, 1278, 1372, 1477
BspMAI CTGCAG 1 cut(s) 474
BspPI GGATC 2 cut(s) 19, 683
BspT107I GGYRCC 1 cut(s) 1475
BspTNI GGTCTC 1 cut(s) 1180
BsrDI GCAATG 5 cut(s) 166, 1105, 1260, 1455, 1630
BsrFI RCCGGY 3 cut(s) 718, 1318, 1336
BssAI RCCGGY 3 cut(s) 718, 1318, 1336
BssECI CCNNGG 5 cut(s) 1078, 1229, 1372, 1387, 1479
BssMI GATC 6 cut(s) 24, 180, 502, 634, 688, 967
BssNI GRCGYC 1 cut(s) 1476
BssT1I CCWWGG 2 cut(s) 1078, 1387
Bst2UI CCWGG 5 cut(s) 543, 698, 1203, 1230, 1413
Bst4CI ACNGT 3 cut(s) 59, 1019, 1437
Bst6I CTCTTC 1 cut(s) 6
BstACI GRCGYC 1 cut(s) 1476
BstAPI GCANNNNNTGC 1 cut(s) 1225
BstC8I GCNNGC 6 cut(s) 71, 192, 720, 1199, 1292, 1320
BstDEI CTNAG 4 cut(s) 384, 414, 909, 1401
BstEII GGTNACC 1 cut(s) 1415
BstF5I GGATG 4 cut(s) 71, 691, 1140, 1239
BstFNI CGCG 1 cut(s) 194
BstH2I RGCGCY 3 cut(s) 228, 849, 1479
BstHHI GCGC 4 cut(s) 227, 848, 904, 1478
BstKTI GATC 6 cut(s) 27, 183, 505, 637, 691, 970
BstMAI GTCTC 2 cut(s) 912, 1180
BstMBI GATC 6 cut(s) 24, 180, 502, 634, 688, 967
BstNI CCWGG 5 cut(s) 543, 698, 1203, 1230, 1413
BstNSI RCATGY 4 cut(s) 212, 289, 1201, 1540
BstPI GGTNACC 1 cut(s) 1415
BstSCI CCNGG 7 cut(s) 541, 696, 1201, 1228, 1372, 1411, 1478
BstSFI CTRYAG 1 cut(s) 470
BstSLI GKGCMC 1 cut(s) 1054
BstUI CGCG 1 cut(s) 194
BstX2I RGATCY 2 cut(s) 24, 502
BstXI CCANNNNNNTGG 2 cut(s) 1085, 1394
BstYI RGATCY 2 cut(s) 24, 502
Bsu36I CCTNAGG 2 cut(s) 384, 1401
BtgZI GCGATG 1 cut(s) 542
BtrI CACGTC 1 cut(s) 598
BtsCI GGATG 4 cut(s) 71, 691, 1140, 1239
BtsIMutI CAGTG 3 cut(s) 55, 1024, 1606
BtuMI TCGCGA 1 cut(s) 194
Cac8I GCNNGC 6 cut(s) 71, 192, 720, 1199, 1292, 1320
CaiI CAGNNNCTG 2 cut(s) 469, 754
CfoI GCGC 4 cut(s) 227, 848, 904, 1478
Cfr10I RCCGGY 3 cut(s) 718, 1318, 1336
Cfr13I GGNCC 8 cut(s) 404, 554, 1259, 1268, 1277, 1309, 1370, 1397
CspCI CAANNNNNGTGG 4 cut(s) 1000, 1035, 1162, 1197
DdeI CTNAG 4 cut(s) 384, 414, 909, 1401
DinI GGCGCC 1 cut(s) 1477
DpnI GATC 6 cut(s) 26, 182, 504, 636, 690, 969
DpnII GATC 6 cut(s) 24, 180, 502, 634, 688, 967
DraI TTTAAA 1 cut(s) 550
DraIII CACNNNGTG 1 cut(s) 79
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 6
EarI CTCTTC 1 cut(s) 6
EciI GGCGGA 2 cut(s) 369, 900
Ecl136II GAGCTC 1 cut(s) 217
Eco130I CCWWGG 2 cut(s) 1078, 1387
Eco24I GRGCYC 1 cut(s) 219
Eco31I GGTCTC 1 cut(s) 1180
Eco47I GGWCC 3 cut(s) 1259, 1268, 1277
Eco53kI GAGCTC 1 cut(s) 217
Eco57I CTGAAG 3 cut(s) 464, 527, 1582
Eco81I CCTNAGG 2 cut(s) 384, 1401
Eco91I GGTNACC 1 cut(s) 1415
EcoICRI GAGCTC 1 cut(s) 217
EcoO109I RGGNCCY 1 cut(s) 1259
EcoO65I GGTNACC 1 cut(s) 1415
EcoRII CCWGG 5 cut(s) 541, 696, 1201, 1228, 1411
EcoT14I CCWWGG 2 cut(s) 1078, 1387
EcoT38I GRGCYC 1 cut(s) 219
EgeI GGCGCC 1 cut(s) 1477
EheI GGCGCC 1 cut(s) 1477
ErhI CCWWGG 2 cut(s) 1078, 1387
Esp3I CGTCTC 1 cut(s) 912
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 1 cut(s) 1496
FbaI TGATCA 1 cut(s) 180
FblI GTMKAC 1 cut(s) 1333
FokI GGATG 4 cut(s) 78, 698, 1147, 1246
FriOI GRGCYC 1 cut(s) 219
FspBI CTAG 1 cut(s) 567
GlaI GCGC 4 cut(s) 226, 847, 903, 1477
GsaI CCCAGC 3 cut(s) 990, 1398, 1549
GsuI CTGGAG 3 cut(s) 446, 1224, 1551
HaeII RGCGCY 3 cut(s) 228, 849, 1479
HapII CCGG 6 cut(s) 156, 719, 1319, 1337, 1374, 1479
HhaI GCGC 4 cut(s) 227, 848, 904, 1478
Hin1I GRCGYC 1 cut(s) 1476
Hin6I GCGC 4 cut(s) 225, 846, 902, 1476
HinP1I GCGC 4 cut(s) 225, 846, 902, 1476
HincII GTYRAC 2 cut(s) 49, 166
HindII GTYRAC 2 cut(s) 49, 166
HindIII AAGCTT 1 cut(s) 1288
HinfI GANTC 1 cut(s) 128
HpaI GTTAAC 1 cut(s) 49
HpaII CCGG 6 cut(s) 156, 719, 1319, 1337, 1374, 1479
HphI GGTGA 2 cut(s) 91, 1589
Hpy166II GTNNAC 6 cut(s) 49, 166, 601, 821, 1334, 1580
Hpy188I TCNGA 8 cut(s) 133, 180, 231, 252, 507, 705, 807, 1305
Hpy188III TCNNGA 5 cut(s) 19, 28, 184, 193, 638
Hpy8I GTNNAC 6 cut(s) 49, 166, 601, 821, 1334, 1580
Hpy99I CGWCG 2 cut(s) 599, 665
HpyAV CCTTC 6 cut(s) 136, 226, 331, 484, 574, 802
HpyCH4III ACNGT 3 cut(s) 59, 1019, 1437
HpyCH4IV ACGT 1 cut(s) 597
HpyF3I CTNAG 4 cut(s) 384, 414, 909, 1401
HpySE526I ACGT 1 cut(s) 597
Hsp92I GRCGYC 1 cut(s) 1476
HspAI GCGC 4 cut(s) 225, 846, 902, 1476
KasI GGCGCC 1 cut(s) 1475
KroI GCCGGC 2 cut(s) 718, 1318
KroNI GCCGGC 2 cut(s) 720, 1320
Ksp22I TGATCA 1 cut(s) 180
KspAI GTTAAC 1 cut(s) 49
Kzo9I GATC 6 cut(s) 24, 180, 502, 634, 688, 967
LmnI GCTCC 3 cut(s) 232, 1103, 1391
LweI GCATC 3 cut(s) 56, 519, 1215
MaeI CTAG 1 cut(s) 567
MaeII ACGT 1 cut(s) 597
MaeIII GTNAC 2 cut(s) 1067, 1415
MalI GATC 6 cut(s) 26, 182, 504, 636, 690, 969
MboI GATC 6 cut(s) 24, 180, 502, 634, 688, 967
MboII GAAGA 6 cut(s) 23, 34, 94, 617, 662, 1588
MfeI CAATTG 1 cut(s) 518
MflI RGATCY 2 cut(s) 24, 502
MhlI GDGCHC 3 cut(s) 40, 219, 1054
MlsI TGGCCA 1 cut(s) 269
MluCI AATT 4 cut(s) 99, 429, 518, 590
MluNI TGGCCA 1 cut(s) 269
Mly113I GGCGCC 1 cut(s) 1476
Mox20I TGGCCA 1 cut(s) 269
MroNI GCCGGC 2 cut(s) 718, 1318
MscI TGGCCA 1 cut(s) 269
MseI TTAA 3 cut(s) 48, 549, 1439
MslI CAYNNNNRTG 2 cut(s) 60, 1253
Msp20I TGGCCA 1 cut(s) 269
MspA1I CMGCKG 3 cut(s) 345, 469, 1571
MspI CCGG 6 cut(s) 156, 719, 1319, 1337, 1374, 1479
MspR9I CCNGG 7 cut(s) 543, 698, 1203, 1230, 1374, 1413, 1480
MunI CAATTG 1 cut(s) 518
MvaI CCWGG 5 cut(s) 543, 698, 1203, 1230, 1413
MvnI CGCG 1 cut(s) 194
NaeI GCCGGC 2 cut(s) 720, 1320
NarI GGCGCC 1 cut(s) 1476
NciI CCSGG 2 cut(s) 1374, 1480
NdeII GATC 6 cut(s) 24, 180, 502, 634, 688, 967
NgoMIV GCCGGC 2 cut(s) 718, 1318
NlaIV GGNNCC 5 cut(s) 1260, 1269, 1278, 1372, 1477
NruI TCGCGA 1 cut(s) 194
NspI RCATGY 4 cut(s) 212, 289, 1201, 1540
PaeI GCATGC 1 cut(s) 1201
PcsI WCGNNNNNNNCGW 1 cut(s) 600
PdiI GCCGGC 2 cut(s) 720, 1320
PfeI GAWTC 1 cut(s) 128
PflMI CCANNNNNTGG 3 cut(s) 1040, 1177, 1574
PluTI GGCGCC 1 cut(s) 1479
PpuMI RGGWCCY 1 cut(s) 1259
Psp124BI GAGCTC 1 cut(s) 219
Psp5II RGGWCCY 1 cut(s) 1259
Psp6I CCWGG 5 cut(s) 541, 696, 1201, 1228, 1411
PspEI GGTNACC 1 cut(s) 1415
PspFI CCCAGC 3 cut(s) 986, 1394, 1545
PspGI CCWGG 5 cut(s) 541, 696, 1201, 1228, 1411
PspN4I GGNNCC 5 cut(s) 1260, 1269, 1278, 1372, 1477
PspPI GGNCC 8 cut(s) 404, 554, 1259, 1268, 1277, 1309, 1370, 1397
PspPPI RGGWCCY 1 cut(s) 1259
PstI CTGCAG 1 cut(s) 474
PstNI CAGNNNCTG 2 cut(s) 469, 754
PsuI RGATCY 2 cut(s) 24, 502
PvuII CAGCTG 2 cut(s) 469, 1571
RruI TCGCGA 1 cut(s) 194
RseI CAYNNNNRTG 2 cut(s) 60, 1253
SacI GAGCTC 1 cut(s) 219
SaqAI TTAA 3 cut(s) 48, 549, 1439
Sau3AI GATC 6 cut(s) 24, 180, 502, 634, 688, 967
Sau96I GGNCC 8 cut(s) 404, 554, 1259, 1268, 1277, 1309, 1370, 1397
ScrFI CCNGG 7 cut(s) 543, 698, 1203, 1230, 1374, 1413, 1480
SduI GDGCHC 3 cut(s) 40, 219, 1054
SfaNI GCATC 3 cut(s) 56, 519, 1215
SfcI CTRYAG 1 cut(s) 470
SfoI GGCGCC 1 cut(s) 1477
SinI GGWCC 3 cut(s) 1259, 1268, 1277
SmiMI CAYNNNNRTG 2 cut(s) 60, 1253
SmlI CTYRAG 1 cut(s) 1191
SmoI CTYRAG 1 cut(s) 1191
SphI GCATGC 1 cut(s) 1201
Sse9I AATT 4 cut(s) 99, 429, 518, 590
SspDI GGCGCC 1 cut(s) 1475
SspMI CTAG 1 cut(s) 567
SstI GAGCTC 1 cut(s) 219
StyD4I CCNGG 7 cut(s) 541, 696, 1201, 1228, 1372, 1411, 1478
StyI CCWWGG 2 cut(s) 1078, 1387
TaaI ACNGT 3 cut(s) 59, 1019, 1437
TaiI ACGT 1 cut(s) 600
TaqI TCGA 2 cut(s) 594, 1526
TasI AATT 4 cut(s) 99, 429, 518, 590
TfiI GAWTC 1 cut(s) 128
Tru1I TTAA 3 cut(s) 48, 549, 1439
Tru9I TTAA 3 cut(s) 48, 549, 1439
TscAI CASTG 3 cut(s) 62, 1024, 1606
TspDTI ATGAA 8 cut(s) 503, 728, 785, 794, 812, 1142, 1259, 1469
TspGWI ACGGA 2 cut(s) 163, 1086
TspRI CASTG 3 cut(s) 62, 1024, 1606
Van91I CCANNNNNTGG 3 cut(s) 1040, 1177, 1574
VpaK11BI GGWCC 3 cut(s) 1259, 1268, 1277
XapI RAATTY 2 cut(s) 429, 590
XceI RCATGY 4 cut(s) 212, 289, 1201, 1540
XcmI CCANNNNNNNNNTGG 1 cut(s) 1178
XmiI GTMKAC 1 cut(s) 1333
XspI CTAG 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.