Rorug04G0152100

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007097
Physical Location & Seq
Forward (+)
26630444 .. 26630926
483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0152100.1

Sequence Viewer

Length: 342 bp
ATGCGGGATCTGGGGATGGTACCAAACCTTGTGGCAGTTAAAGATCACAATGGCAATCCTGTTGTGGCTAGTTCAAGATGTATTGGTAGTGCTTCTGTTCCTTTGGCAGAATGCACTGCTTTAAAGGATGGTATGTTGGCTGCTCATCAATTGAACTTAAAGAATATTGGAGTCGAAGGAGATTCTGCTCTAATTATCAATTGTGTCAAGCTCCAATGTGAAGTTCCTAGAACTTTGAAATCCTTGACAAGAGACATGTCTGCCATCTCGAATCTGTTCAACTACCTTCTCCCATGTTTTCCAAGAAGCCAATTTCTTGGCAGATGCTATCACGGTTGCTGA

Protein Analysis

113

Amino Acids

12.2

Weight (kDa)

8.54

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 12 - 94 6.1e-09 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 19
AccB1I GGYRCC 1 cut(s) 19
AciI CCGC 1 cut(s) 4
AclWI GGATC 1 cut(s) 15
AfaI GTAC 1 cut(s) 21
AflIII ACRYGT 1 cut(s) 255
AgsI TTSAA 4 cut(s) 75, 154, 238, 280
AjuI GAANNNNNNNTTGG 2 cut(s) 207, 239
AluBI AGCT 1 cut(s) 211
AluI AGCT 1 cut(s) 211
Alw26I GTCTC 1 cut(s) 246
AlwI GGATC 1 cut(s) 15
ApeKI GCWGC 1 cut(s) 140
Asp700I GAANNNNTTC 1 cut(s) 275
Asp718I GGTACC 1 cut(s) 19
BanI GGYRCC 1 cut(s) 19
BbvI GCAGC 1 cut(s) 127
BccI CCATC 3 cut(s) 10, 122, 272
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 2 cut(s) 69, 228
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
BmiI GGNNCC 1 cut(s) 21
BmsI GCATC 1 cut(s) 314
BseGI GGATG 2 cut(s) 21, 133
BseXI GCAGC 1 cut(s) 127
BshNI GGYRCC 1 cut(s) 19
BsmAI GTCTC 1 cut(s) 246
BsmI GAATGC 1 cut(s) 116
Bsp143I GATC 2 cut(s) 7, 43
BspACI CCGC 1 cut(s) 4
BspLI GGNNCC 1 cut(s) 21
BspPI GGATC 1 cut(s) 15
BspT107I GGYRCC 1 cut(s) 19
BssMI GATC 2 cut(s) 7, 43
Bst4CI ACNGT 1 cut(s) 335
BstF5I GGATG 2 cut(s) 21, 133
BstKTI GATC 2 cut(s) 10, 46
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 2 cut(s) 7, 43
BstNSI RCATGY 1 cut(s) 259
BstV1I GCAGC 1 cut(s) 127
BstX2I RGATCY 1 cut(s) 7
BstXI CCANNNNNNTGG 1 cut(s) 317
BstYI RGATCY 1 cut(s) 7
BtsCI GGATG 2 cut(s) 21, 133
BtsI GCAGTG 1 cut(s) 114
BtsIMutI CAGTG 1 cut(s) 114
Csp6I GTAC 1 cut(s) 20
CspCI CAANNNNNGTGG 2 cut(s) 12, 47
CviAII CATG 2 cut(s) 256, 294
CviJI RGCY 4 cut(s) 68, 140, 211, 309
CviKI_1 RGCY 4 cut(s) 68, 140, 211, 309
CviQI GTAC 1 cut(s) 20
DpnI GATC 2 cut(s) 9, 45
DpnII GATC 2 cut(s) 7, 43
DraI TTTAAA 1 cut(s) 123
FaeI CATG 2 cut(s) 259, 297
FaiI YATR 3 cut(s) 134, 257, 295
FatI CATG 2 cut(s) 255, 293
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 2 cut(s) 28, 140
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 2 cut(s) 69, 228
GluI GCNGC 1 cut(s) 141
Hin1II CATG 2 cut(s) 259, 297
HinfI GANTC 3 cut(s) 171, 182, 271
Hpy188III TCNNGA 2 cut(s) 75, 268
HpyAV CCTTC 2 cut(s) 170, 296
HpyCH4III ACNGT 1 cut(s) 335
HpyCH4V TGCA 1 cut(s) 114
Hsp92II CATG 2 cut(s) 259, 297
KpnI GGTACC 1 cut(s) 23
Kzo9I GATC 2 cut(s) 7, 43
LmnI GCTCC 1 cut(s) 216
LpnPI CCDG 1 cut(s) 72
Lsp1109I GCAGC 1 cut(s) 127
LweI GCATC 1 cut(s) 314
MaeI CTAG 2 cut(s) 69, 228
MalI GATC 2 cut(s) 9, 45
MboI GATC 2 cut(s) 7, 43
MfeI CAATTG 2 cut(s) 149, 199
MflI RGATCY 1 cut(s) 7
MluCI AATT 4 cut(s) 149, 192, 199, 311
MlyI GAGTC 1 cut(s) 180
MroXI GAANNNNTTC 1 cut(s) 275
MseI TTAA 3 cut(s) 39, 122, 158
MunI CAATTG 2 cut(s) 149, 199
Mva1269I GAATGC 1 cut(s) 116
NdeII GATC 2 cut(s) 7, 43
NlaIII CATG 2 cut(s) 259, 297
NlaIV GGNNCC 1 cut(s) 21
NspI RCATGY 1 cut(s) 259
PciI ACATGT 1 cut(s) 255
PctI GAATGC 1 cut(s) 116
PdmI GAANNNNTTC 1 cut(s) 275
PfeI GAWTC 2 cut(s) 182, 271
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
PscI ACATGT 1 cut(s) 255
PspN4I GGNNCC 1 cut(s) 21
PsuI RGATCY 1 cut(s) 7
RsaI GTAC 1 cut(s) 21
RsaNI GTAC 1 cut(s) 20
SaqAI TTAA 3 cut(s) 39, 122, 158
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 2 cut(s) 7, 43
SchI GAGTC 1 cut(s) 180
SetI ASST 3 cut(s) 30, 213, 288
SfaNI GCATC 1 cut(s) 314
Sse9I AATT 4 cut(s) 149, 192, 199, 311
SsiI CCGC 1 cut(s) 4
SspI AATATT 1 cut(s) 166
SspMI CTAG 2 cut(s) 69, 228
TaaI ACNGT 1 cut(s) 335
TaqI TCGA 2 cut(s) 174, 269
TasI AATT 4 cut(s) 149, 192, 199, 311
TfiI GAWTC 2 cut(s) 182, 271
Tru1I TTAA 3 cut(s) 39, 122, 158
Tru9I TTAA 3 cut(s) 39, 122, 158
TscAI CASTG 1 cut(s) 121
TseI GCWGC 1 cut(s) 140
TspRI CASTG 1 cut(s) 121
XceI RCATGY 1 cut(s) 259
XmnI GAANNNNTTC 1 cut(s) 275
XspI CTAG 2 cut(s) 69, 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.