Rw4G018140

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0007097
Physical Location & Seq
Reverse (-)
40365284 .. 40367207
1924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G018140.1

Sequence Viewer

Length: 1572 bp
ATGAGTAAAGAAGAGTTCATGAAGATCCAGAAATGTACTCTCAAAGTTAACATTCACTGTGATGGATGCAAGCACAAGGTGAAGAAAATCTTGCAGAGAATTGAAGGGGTTTGTACAACCGACATAGATTCCGAGCAGGGGAAGGTGATCGTTTCCGGCAATGTTGACCCCTCCGTTCTGATCAAGAAGCTCGCGAAATCAGGGAAACGTGCAGAGCTCTGGGGCGCTCCGAAGGCCAACAACAACAATCAGAACCAGAACAACATGGCCAATCAGAAGAACATGCAACTTGACAATGGCAAAGGTGGGAATAACAAAGGCCAAAACAACCAGAAGGGCGGTGGCGGTGGCGGAGGGGGAGCCGGAGGAGGAGGAGGCAACAACAATCAGCCTAAGGGTGGAGGACAACAAGGCCCAAACCCTCAGCAACAGCAACAAATGCAGCAGCAAATGCAGCAGCAACTCCTGCAGCTGCAGCAGCTTCAGCAAATGAAGGCGTTGCAAGATCCGAAGCTGCCCCAATTGAAAGGGATGCAAATGCCTCCTGGTTTCAAAGGCCCGAACCCACCTAGCCAGAACCAGAAGGCTGTGAAATTCGCGGACATGGACGATGATGACATGACTGATGATGAGTATGATGATCTTGATGATGACGAAGATTATGACGACGAGGATTATGATGACGAGCTGGATGATCCTCGCCTGGCTCTGAATAAGATGAAGCCGGCGACCATGGGTGGTGGTGGTGGTCAGGGGCTGCCTCCTATGATGCTGAATGGAATGAACCTCCCACAGATGATGAATGTTCAGAAGGCTGGTGGTGGACAACAGAATGGTGGCGGCGGCGGTGGTGGTAATGGTGGAAAGAAAGGTGGGGAATATGGGCGTGGCGGTGGCGGTGGCGGTGGAAAGAAAGGCGGTGGCGGTGGCGGTGGTGGAGATCATGGTCAAAATCAAACTGGGGGTGGCGGCAAAAATGTAGGCAAGAACGGTGGTGGGTTTCCCCCAAATGGTGGTGGTGGGGCACAGAACAAGAATGGTAACGGAGGCCAAGGTGGTGGAGCTAAAAAGGGTGGTGGTGGTGGTGGAATGAGTGAGGCTCAACTTCATGCTATGATGAGCCACCAGAACATGGGTGGGAGACCTCAAGGCATGCCTGGAGGTAATGTGGGGCAGCAGATGCCTGGGATGAGCCAGATGAACATGGCAATGGGTCCAATAGGTCCAATGGGAAGCATGCCAATGGGTCAGATGGGCCAAATGCCGGCCGTTCAAGGTCTACCGGCTGCCGCCATGAGTGGCGGCGGCCCCGGTGGCTACTTCCAAGGAGCTGGGCCTGAGGCCATGCCTGGTAACCCATACCAGAACCAACAGTTAATGGCTGCAATGATGAACCAGCAGAGAGCTATGGGCGGCGGGGACAGGTCGTTCCAGCCTATGATGTATAATAGGATTCCTCCATCGACCAACTACATGCCGCCCCAGCATTACCCATACCCTCCTCCAGCTGGTGGTGACCCCTACACCCATTTCTTCAGTGATGAGAACACTTCAAGCTGCAATGTGATGTGA

Protein Analysis

523

Amino Acids

53.96

Weight (kDa)

8.09

Isoelectric Point (pI)

57.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 13 - 69 1.8e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 1013, 1132, 1511
AccI GTMKAC 1 cut(s) 1279
AccII CGCG 2 cut(s) 194, 599
AclWI GGATC 3 cut(s) 19, 500, 689
AcoI YGGCCR 2 cut(s) 267, 1266
AcsI RAATTY 1 cut(s) 593
AcuI CTGAAG 2 cut(s) 467, 1519
AdeI CACNNNGTG 1 cut(s) 79
AfaI GTAC 2 cut(s) 37, 115
AfiI CCNNNNNNNGG 8 cut(s) 138, 398, 1010, 1013, 1132, 1264, 1508, 1511
AgsI TTSAA 5 cut(s) 104, 526, 553, 1274, 1554
AjnI CCWGG 5 cut(s) 544, 702, 1156, 1183, 1348
AloI GAACNNNNNNTCC 2 cut(s) 1412, 1444
Alw21I GWGCWC 1 cut(s) 219
Alw26I GTCTC 1 cut(s) 1135
AlwI GGATC 3 cut(s) 19, 500, 689
AlwNI CAGNNNCTG 1 cut(s) 757
ApoI RAATTY 1 cut(s) 593
AspLEI GCGC 1 cut(s) 227
AspS9I GGNCC 7 cut(s) 413, 557, 1214, 1223, 1255, 1307, 1334
AsuC2I CCSGG 1 cut(s) 1311
AsuHPI GGTGA 3 cut(s) 91, 157, 1526
AvaII GGWCC 2 cut(s) 1214, 1223
AxyI CCTNAGG 2 cut(s) 393, 1338
BaeGI GKGCMC 1 cut(s) 1027
BalI TGGCCA 1 cut(s) 269
BanII GRGCYC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 219
BbvCI CCTCAGC 1 cut(s) 423
BccI CCATC 3 cut(s) 56, 1246, 1468
BceAI ACGGC 1 cut(s) 1253
BciT130I CCWGG 5 cut(s) 546, 704, 1158, 1185, 1350
BclI TGATCA 1 cut(s) 180
BcnI CCSGG 1 cut(s) 1311
BcoDI GTCTC 1 cut(s) 1135
BfaI CTAG 1 cut(s) 570
BfmI CTRYAG 2 cut(s) 467, 473
BfoI RGCGCY 1 cut(s) 228
BglI GCCNNNNNGGC 1 cut(s) 1314
Bme1390I CCNGG 6 cut(s) 546, 704, 1158, 1185, 1311, 1350
Bme18I GGWCC 2 cut(s) 1214, 1223
BmgT120I GGNCC 7 cut(s) 413, 557, 1214, 1223, 1255, 1307, 1334
BmiI GGNNCC 3 cut(s) 361, 1215, 1309
BmrFI CCNGG 6 cut(s) 546, 704, 1158, 1185, 1311, 1350
BmrI ACTGGG 1 cut(s) 969
BmsI GCATC 4 cut(s) 56, 522, 759, 1170
BmuI ACTGGG 1 cut(s) 969
BplI GAGNNNNNCTC 2 cut(s) 1084, 1116
BpmI CTGGAG 2 cut(s) 1179, 1488
Bpu10I CCTNAGC 1 cut(s) 423
BpuEI CTTGAG 1 cut(s) 1131
BpuMI CCSGG 1 cut(s) 1311
BsaI GGTCTC 1 cut(s) 1135
BsaJI CCNNGG 5 cut(s) 732, 1051, 1184, 1309, 1324
BsaXI ACNNNNNCTCC 2 cut(s) 1152, 1182
Bsc4I CCNNNNNNNGG 8 cut(s) 138, 398, 1010, 1013, 1132, 1264, 1508, 1511
Bse118I RCCGGY 3 cut(s) 724, 1264, 1282
Bse1I ACTGG 1 cut(s) 964
Bse21I CCTNAGG 2 cut(s) 393, 1338
Bse3DI GCAATG 4 cut(s) 166, 1215, 1392, 1567
BseBI CCWGG 5 cut(s) 546, 704, 1158, 1185, 1350
BseDI CCNNGG 5 cut(s) 732, 1051, 1184, 1309, 1324
BseGI GGATG 4 cut(s) 71, 537, 697, 1194
BseLI CCNNNNNNNGG 8 cut(s) 138, 398, 1010, 1013, 1132, 1264, 1508, 1511
BseMI GCAATG 4 cut(s) 166, 1215, 1392, 1567
BseMII CTCAG 2 cut(s) 437, 1329
BseNI ACTGG 1 cut(s) 964
BseRI GAGGAG 4 cut(s) 381, 384, 387, 1491
BseSI GKGCMC 1 cut(s) 1027
BseX3I CGGCCG 1 cut(s) 1266
BseYI CCCAGC 2 cut(s) 1331, 1482
BsgI GTGCAG 1 cut(s) 231
Bsh1236I CGCG 2 cut(s) 194, 599
Bsh1285I CGRYCG 1 cut(s) 1269
BsiEI CGRYCG 1 cut(s) 1269
BsiHKAI GWGCWC 1 cut(s) 219
BsiSI CCGG 6 cut(s) 156, 363, 725, 1265, 1283, 1311
BslFI GGGAC 1 cut(s) 1433
BslI CCNNNNNNNGG 8 cut(s) 138, 398, 1010, 1013, 1132, 1264, 1508, 1511
BsmAI GTCTC 1 cut(s) 1135
BsmFI GGGAC 1 cut(s) 1433
Bso31I GGTCTC 1 cut(s) 1135
Bsp1286I GDGCHC 2 cut(s) 219, 1027
Bsp1407I TGTACA 1 cut(s) 113
Bsp143I GATC 7 cut(s) 24, 147, 180, 505, 640, 694, 940
Bsp19I CCATGG 1 cut(s) 732
Bsp68I TCGCGA 1 cut(s) 194
BspCNI CTCAG 2 cut(s) 436, 1330
BspFNI CGCG 2 cut(s) 194, 599
BspHI TCATGA 1 cut(s) 18
BspLI GGNNCC 3 cut(s) 361, 1215, 1309
BspMAI CTGCAG 2 cut(s) 471, 477
BspPI GGATC 3 cut(s) 19, 500, 689
BspTNI GGTCTC 1 cut(s) 1135
BsrDI GCAATG 4 cut(s) 166, 1215, 1392, 1567
BsrFI RCCGGY 3 cut(s) 724, 1264, 1282
BsrGI TGTACA 1 cut(s) 113
BsrI ACTGG 1 cut(s) 964
BssAI RCCGGY 3 cut(s) 724, 1264, 1282
BssECI CCNNGG 5 cut(s) 732, 1051, 1184, 1309, 1324
BssMI GATC 7 cut(s) 24, 147, 180, 505, 640, 694, 940
BssT1I CCWWGG 3 cut(s) 732, 1051, 1324
Bst2UI CCWGG 5 cut(s) 546, 704, 1158, 1185, 1350
Bst4CI ACNGT 3 cut(s) 59, 992, 1374
Bst6I CTCTTC 1 cut(s) 6
BstAPI GCANNNNNTGC 4 cut(s) 439, 451, 466, 1180
BstAUI TGTACA 1 cut(s) 113
BstC8I GCNNGC 6 cut(s) 71, 192, 726, 1154, 1238, 1266
BstDEI CTNAG 3 cut(s) 393, 423, 1338
BstDSI CCRYGG 1 cut(s) 732
BstEII GGTNACC 2 cut(s) 1352, 1514
BstF5I GGATG 4 cut(s) 71, 537, 697, 1194
BstFNI CGCG 2 cut(s) 194, 599
BstH2I RGCGCY 1 cut(s) 228
BstHHI GCGC 1 cut(s) 227
BstKTI GATC 7 cut(s) 27, 150, 183, 508, 643, 697, 943
BstMAI GTCTC 1 cut(s) 1135
BstMBI GATC 7 cut(s) 24, 147, 180, 505, 640, 694, 940
BstMCI CGRYCG 1 cut(s) 1269
BstNI CCWGG 5 cut(s) 546, 704, 1158, 1185, 1350
BstNSI RCATGY 4 cut(s) 286, 1156, 1240, 1477
BstPI GGTNACC 2 cut(s) 1352, 1514
BstSCI CCNGG 6 cut(s) 544, 702, 1156, 1183, 1309, 1348
BstSFI CTRYAG 2 cut(s) 467, 473
BstSLI GKGCMC 1 cut(s) 1027
BstUI CGCG 2 cut(s) 194, 599
BstX2I RGATCY 2 cut(s) 24, 505
BstXI CCANNNNNNTGG 2 cut(s) 1058, 1331
BstYI RGATCY 2 cut(s) 24, 505
BstZI CGGCCG 1 cut(s) 1266
Bsu36I CCTNAGG 2 cut(s) 393, 1338
BtgI CCRYGG 1 cut(s) 732
BtsCI GGATG 4 cut(s) 71, 537, 697, 1194
BtsIMutI CAGTG 2 cut(s) 55, 1543
BtuMI TCGCGA 1 cut(s) 194
Cac8I GCNNGC 6 cut(s) 71, 192, 726, 1154, 1238, 1266
CaiI CAGNNNCTG 1 cut(s) 757
CciI TCATGA 1 cut(s) 18
CfoI GCGC 1 cut(s) 227
Cfr10I RCCGGY 3 cut(s) 724, 1264, 1282
Cfr13I GGNCC 7 cut(s) 413, 557, 1214, 1223, 1255, 1307, 1334
Csp6I GTAC 2 cut(s) 36, 114
CspCI CAANNNNNGTGG 2 cut(s) 973, 1008
CviQI GTAC 2 cut(s) 36, 114
DdeI CTNAG 3 cut(s) 393, 423, 1338
DpnI GATC 7 cut(s) 26, 149, 182, 507, 642, 696, 942
DpnII GATC 7 cut(s) 24, 147, 180, 505, 640, 694, 940
DraIII CACNNNGTG 1 cut(s) 79
EaeI YGGCCR 2 cut(s) 267, 1266
EagI CGGCCG 1 cut(s) 1266
Eam1104I CTCTTC 1 cut(s) 6
EarI CTCTTC 1 cut(s) 6
EciI GGCGGA 1 cut(s) 366
Ecl136II GAGCTC 1 cut(s) 217
EclXI CGGCCG 1 cut(s) 1266
Eco130I CCWWGG 3 cut(s) 732, 1051, 1324
Eco24I GRGCYC 1 cut(s) 219
Eco31I GGTCTC 1 cut(s) 1135
Eco47I GGWCC 2 cut(s) 1214, 1223
Eco52I CGGCCG 1 cut(s) 1266
Eco53kI GAGCTC 1 cut(s) 217
Eco57I CTGAAG 2 cut(s) 467, 1519
Eco81I CCTNAGG 2 cut(s) 393, 1338
Eco91I GGTNACC 2 cut(s) 1352, 1514
EcoICRI GAGCTC 1 cut(s) 217
EcoO65I GGTNACC 2 cut(s) 1352, 1514
EcoRII CCWGG 5 cut(s) 544, 702, 1156, 1183, 1348
EcoT14I CCWWGG 3 cut(s) 732, 1051, 1324
EcoT38I GRGCYC 1 cut(s) 219
ErhI CCWWGG 3 cut(s) 732, 1051, 1324
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 1 cut(s) 1433
FauI CCCGC 1 cut(s) 1409
FbaI TGATCA 1 cut(s) 180
FblI GTMKAC 1 cut(s) 1279
FokI GGATG 4 cut(s) 78, 544, 704, 1201
FriOI GRGCYC 1 cut(s) 219
FspBI CTAG 1 cut(s) 570
GlaI GCGC 1 cut(s) 226
GsaI CCCAGC 2 cut(s) 1335, 1486
GsuI CTGGAG 2 cut(s) 1179, 1488
HaeII RGCGCY 1 cut(s) 228
HapII CCGG 6 cut(s) 156, 363, 725, 1265, 1283, 1311
HhaI GCGC 1 cut(s) 227
Hin6I GCGC 1 cut(s) 225
HinP1I GCGC 1 cut(s) 225
HincII GTYRAC 2 cut(s) 49, 166
HindII GTYRAC 2 cut(s) 49, 166
HinfI GANTC 2 cut(s) 128, 1453
HpaI GTTAAC 1 cut(s) 49
HpaII CCGG 6 cut(s) 156, 363, 725, 1265, 1283, 1311
HphI GGTGA 3 cut(s) 91, 157, 1526
Hpy166II GTNNAC 4 cut(s) 49, 166, 824, 1280
Hpy188I TCNGA 9 cut(s) 133, 180, 231, 252, 276, 510, 711, 810, 1251
Hpy188III TCNNGA 5 cut(s) 19, 28, 184, 193, 644
Hpy8I GTNNAC 4 cut(s) 49, 166, 824, 1280
Hpy99I CGWCG 1 cut(s) 671
HpyAV CCTTC 7 cut(s) 98, 136, 226, 328, 487, 577, 805
HpyCH4III ACNGT 3 cut(s) 59, 992, 1374
HpyCH4IV ACGT 1 cut(s) 208
HpyF3I CTNAG 3 cut(s) 393, 423, 1338
HpySE526I ACGT 1 cut(s) 208
HspAI GCGC 1 cut(s) 225
KroI GCCGGC 2 cut(s) 724, 1264
KroNI GCCGGC 2 cut(s) 726, 1266
Ksp22I TGATCA 1 cut(s) 180
KspAI GTTAAC 1 cut(s) 49
Kzo9I GATC 7 cut(s) 24, 147, 180, 505, 640, 694, 940
LmnI GCTCC 4 cut(s) 232, 359, 1061, 1328
LweI GCATC 4 cut(s) 56, 522, 759, 1170
MaeI CTAG 1 cut(s) 570
MaeII ACGT 1 cut(s) 208
MaeIII GTNAC 3 cut(s) 1040, 1352, 1514
MalI GATC 7 cut(s) 26, 149, 182, 507, 642, 696, 942
MboI GATC 7 cut(s) 24, 147, 180, 505, 640, 694, 940
MboII GAAGA 6 cut(s) 23, 34, 94, 289, 668, 1525
MfeI CAATTG 1 cut(s) 521
MflI RGATCY 2 cut(s) 24, 505
MhlI GDGCHC 2 cut(s) 219, 1027
MlsI TGGCCA 1 cut(s) 269
MluCI AATT 3 cut(s) 99, 521, 593
MluNI TGGCCA 1 cut(s) 269
Mox20I TGGCCA 1 cut(s) 269
MroNI GCCGGC 2 cut(s) 724, 1264
MscI TGGCCA 1 cut(s) 269
MseI TTAA 2 cut(s) 48, 1376
MslI CAYNNNNRTG 3 cut(s) 60, 1208, 1241
Msp20I TGGCCA 1 cut(s) 269
MspA1I CMGCKG 2 cut(s) 472, 1508
MspI CCGG 6 cut(s) 156, 363, 725, 1265, 1283, 1311
MspR9I CCNGG 6 cut(s) 546, 704, 1158, 1185, 1311, 1350
MunI CAATTG 1 cut(s) 521
MvaI CCWGG 5 cut(s) 546, 704, 1158, 1185, 1350
MvnI CGCG 2 cut(s) 194, 599
NaeI GCCGGC 2 cut(s) 726, 1266
NciI CCSGG 1 cut(s) 1311
NcoI CCATGG 1 cut(s) 732
NdeII GATC 7 cut(s) 24, 147, 180, 505, 640, 694, 940
NgoMIV GCCGGC 2 cut(s) 724, 1264
NlaIV GGNNCC 3 cut(s) 361, 1215, 1309
NmuCI GTSAC 1 cut(s) 1514
NruI TCGCGA 1 cut(s) 194
NspI RCATGY 4 cut(s) 286, 1156, 1240, 1477
PaeI GCATGC 2 cut(s) 1156, 1240
PagI TCATGA 1 cut(s) 18
PdiI GCCGGC 2 cut(s) 726, 1266
PfeI GAWTC 2 cut(s) 128, 1453
PflMI CCANNNNNTGG 3 cut(s) 1013, 1132, 1511
Psp124BI GAGCTC 1 cut(s) 219
Psp6I CCWGG 5 cut(s) 544, 702, 1156, 1183, 1348
PspEI GGTNACC 2 cut(s) 1352, 1514
PspFI CCCAGC 2 cut(s) 1331, 1482
PspGI CCWGG 5 cut(s) 544, 702, 1156, 1183, 1348
PspN4I GGNNCC 3 cut(s) 361, 1215, 1309
PspPI GGNCC 7 cut(s) 413, 557, 1214, 1223, 1255, 1307, 1334
PstI CTGCAG 2 cut(s) 471, 477
PstNI CAGNNNCTG 1 cut(s) 757
PsuI RGATCY 2 cut(s) 24, 505
PvuII CAGCTG 2 cut(s) 472, 1508
RruI TCGCGA 1 cut(s) 194
RsaI GTAC 2 cut(s) 37, 115
RsaNI GTAC 2 cut(s) 36, 114
RseI CAYNNNNRTG 3 cut(s) 60, 1208, 1241
SacI GAGCTC 1 cut(s) 219
SaqAI TTAA 2 cut(s) 48, 1376
Sau3AI GATC 7 cut(s) 24, 147, 180, 505, 640, 694, 940
Sau96I GGNCC 7 cut(s) 413, 557, 1214, 1223, 1255, 1307, 1334
ScrFI CCNGG 6 cut(s) 546, 704, 1158, 1185, 1311, 1350
SduI GDGCHC 2 cut(s) 219, 1027
SfaNI GCATC 4 cut(s) 56, 522, 759, 1170
SfcI CTRYAG 2 cut(s) 467, 473
SinI GGWCC 2 cut(s) 1214, 1223
SmiMI CAYNNNNRTG 3 cut(s) 60, 1208, 1241
SmlI CTYRAG 1 cut(s) 1146
SmoI CTYRAG 1 cut(s) 1146
SphI GCATGC 2 cut(s) 1156, 1240
Sse9I AATT 3 cut(s) 99, 521, 593
SspMI CTAG 1 cut(s) 570
SstI GAGCTC 1 cut(s) 219
StyD4I CCNGG 6 cut(s) 544, 702, 1156, 1183, 1309, 1348
StyI CCWWGG 3 cut(s) 732, 1051, 1324
TaaI ACNGT 3 cut(s) 59, 992, 1374
TaiI ACGT 1 cut(s) 211
TaqI TCGA 1 cut(s) 1463
TasI AATT 3 cut(s) 99, 521, 593
TatI WGTACW 2 cut(s) 35, 113
TauI GCSGC 8 cut(s) 843, 846, 972, 1292, 1305, 1308, 1416, 1480
TfiI GAWTC 2 cut(s) 128, 1453
Tru1I TTAA 2 cut(s) 48, 1376
Tru9I TTAA 2 cut(s) 48, 1376
TscAI CASTG 2 cut(s) 62, 1543
TseFI GTSAC 1 cut(s) 1514
Tsp45I GTSAC 1 cut(s) 1514
TspDTI ATGAA 9 cut(s) 7, 35, 506, 734, 797, 815, 1097, 1214, 1406
TspGWI ACGGA 2 cut(s) 163, 1059
TspRI CASTG 2 cut(s) 62, 1543
Van91I CCANNNNNTGG 3 cut(s) 1013, 1132, 1511
VpaK11BI GGWCC 2 cut(s) 1214, 1223
XapI RAATTY 1 cut(s) 593
XceI RCATGY 4 cut(s) 286, 1156, 1240, 1477
XcmI CCANNNNNNNNNTGG 2 cut(s) 338, 1133
XmiI GTMKAC 1 cut(s) 1279
XspI CTAG 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.