Rh4DG212100

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007097
Physical Location & Seq
Reverse (-)
40732408 .. 40735273
2866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG212100.1

Sequence Viewer

Length: 1587 bp
ATGCAGAAATGTACTCTCAAAGTTAACATTCACTGTGATGGATGCAAGCACAAGGTGAAGAAAATCTTGCAGAGAATTGAAGGGGTTTGTACAACCGACATAGATTCCGAGCAGGGGAAGGTGATCGTTTCCGGCAATGTTGACCCCTCCGTTCTGATCAAGAAGCTCGCGAAATCAGGGAAACGTGCAGAGCTCTGGGGCGCTCCGAAGGCCAACAACAACAATCAGAACCAGAACAACATGGCCAATCAGAAGAACATGCAACTTGACAATGGCAAAGGTGGGAATAACAAAGGCCAAAACAACCAGAAGGGCGGTGGCGGTGGCGGAGGGGGAGCCGGAGGAGGAGGAGGCAACAACAATCAGCCTAAGGGTGGAGGACAACAAGGCCCAAACCCTCAGCAACAGCAACAAATGCAGCAGCAAATGCAGCAGCAACTCCTGCAGCTGCAGCAGCTTCAGCAAATGAAGGCGTTGCAAGATCCGAAGCTGCCCCAATTGAAAGGGATGCAAATGCCTCCTGGTTTCAAAGGCCTGAACCCACCTAGCCAGAACCAGAAGGCTGTGAAATTCGCGGACATGGACGATGATGACATGACTGATGATGAGTATGATGATCTTGATGATGACGAAGATTTTGACGACGAGGATTATGATGACGAGCTGGATGATCCTCGCCTGGCTCTGAATAAGATGAAGCCGGCGACCATGGGTGGTGGTGGTGGTCAGGGGCTGCCTCCTATGATGCTGAATGGAATGAACCTCCCACAGATGATGAATGTTCAGAAGGCTGGTGGTGGACAACAGAATGGTGGCGGCGGCGGTGGTGGTAATGGTGGAAAGAAAGGTGGTGGCGGCGGCGGAGGAGGGGGTGCGCCGTCTCAGGGGAATATGGGCGGTGGCGGTGGCGGTGGAAAGAAAGGCGGTGGCGGTGGCGGTGGTGGAGATCATGGTCAAAATCAAACTGGGGGTGGCGGCAAAAATGTAGGCAAGAACGGTGGTGGGTTTCCCCCAAATGGTGGTGGTGGGGCACAGAACAAGAATGGTAACGGAGGCCAAGGTGGTGGAGCTAAAAAGGGTGGTGGTGGTGGTGGAATGAGTGAGGCTCAACTTCATGCTATGATGAGCCACCAGAACATGGGTGGGAGACCTCAAGGCATGCCTGGAGGTAATGTGGGGCAGCAGATGCCTGGGATGAGCCAGATGAACATGGCAATGGGTCCAATAGGTCCAATGGGAAGCATGCCAATGGGTCAGATGGGCCAAATGCCGGCCGTTCAAGGTCTACCGGCTGCCGCCATGAGCGGCGGCGGCGGCGGCGGCCCCGGTGGCTACTTCCAAGGAGCTGGGCCTGAGGCCATGCCTGGTAACCCATACCAGAACCCACAGTTAATGGCTGCAATGATGAACCAGCAGAGAGCTATGGGCGGCGGGGACAGGTCGTTCCAGCCTATGATGTATAATAGGATTCCTCCATCGACCAACTACATGCCGCCCCAGCATTACCCATACCCTCCTCCAGCTGGTGGTGACCCCTACACCCATTTCTTCAGTGATGAGAACACTTCAAGCTGCAATGTGATGTGA

Protein Analysis

528

Amino Acids

53.68

Weight (kDa)

8.11

Isoelectric Point (pI)

59.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 5 - 61 1.8e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 1019, 1138, 1526
AccBSI CCGCTC 1 cut(s) 1305
AccI GTMKAC 1 cut(s) 1285
AccII CGCG 2 cut(s) 170, 575
AclWI GGATC 2 cut(s) 476, 665
AcoI YGGCCR 2 cut(s) 243, 1272
AcsI RAATTY 1 cut(s) 569
AcuI CTGAAG 2 cut(s) 443, 1534
AdeI CACNNNGTG 1 cut(s) 55
AfaI GTAC 2 cut(s) 13, 91
AfiI CCNNNNNNNGG 9 cut(s) 114, 374, 884, 1016, 1019, 1138, 1270, 1523, 1526
AgsI TTSAA 5 cut(s) 80, 502, 529, 1280, 1569
AjnI CCWGG 5 cut(s) 520, 678, 1162, 1189, 1363
AloI GAACNNNNNNTCC 2 cut(s) 1427, 1459
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 2 cut(s) 885, 1141
AlwI GGATC 2 cut(s) 476, 665
AlwNI CAGNNNCTG 1 cut(s) 733
ApoI RAATTY 1 cut(s) 569
ArsI GACNNNNNNTTYG 2 cut(s) 620, 652
AspLEI GCGC 2 cut(s) 203, 877
AspS9I GGNCC 6 cut(s) 389, 1220, 1229, 1261, 1322, 1349
AsuC2I CCSGG 1 cut(s) 1326
AsuHPI GGTGA 3 cut(s) 67, 133, 1541
AvaII GGWCC 2 cut(s) 1220, 1229
AxyI CCTNAGG 2 cut(s) 369, 1353
BaeGI GKGCMC 1 cut(s) 1033
BalI TGGCCA 1 cut(s) 245
BanII GRGCYC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 195
BbvCI CCTCAGC 1 cut(s) 399
BccI CCATC 3 cut(s) 32, 1252, 1483
BceAI ACGGC 2 cut(s) 862, 1259
BciT130I CCWGG 5 cut(s) 522, 680, 1164, 1191, 1365
BclI TGATCA 1 cut(s) 156
BcnI CCSGG 1 cut(s) 1326
BcoDI GTCTC 2 cut(s) 885, 1141
BfaI CTAG 1 cut(s) 546
BfmI CTRYAG 2 cut(s) 443, 449
BfoI RGCGCY 1 cut(s) 204
BglI GCCNNNNNGGC 1 cut(s) 1329
Bme1390I CCNGG 6 cut(s) 522, 680, 1164, 1191, 1326, 1365
Bme18I GGWCC 2 cut(s) 1220, 1229
BmgT120I GGNCC 6 cut(s) 389, 1220, 1229, 1261, 1322, 1349
BmiI GGNNCC 3 cut(s) 337, 1221, 1324
BmrFI CCNGG 6 cut(s) 522, 680, 1164, 1191, 1326, 1365
BmrI ACTGGG 1 cut(s) 975
BmsI GCATC 4 cut(s) 32, 498, 735, 1176
BmuI ACTGGG 1 cut(s) 975
BplI GAGNNNNNCTC 2 cut(s) 1090, 1122
BpmI CTGGAG 2 cut(s) 1185, 1503
Bpu10I CCTNAGC 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 1137
BpuMI CCSGG 1 cut(s) 1326
BsaI GGTCTC 1 cut(s) 1141
BsaJI CCNNGG 5 cut(s) 708, 1057, 1190, 1324, 1339
BsaXI ACNNNNNCTCC 2 cut(s) 1158, 1188
Bsc4I CCNNNNNNNGG 9 cut(s) 114, 374, 884, 1016, 1019, 1138, 1270, 1523, 1526
Bse118I RCCGGY 3 cut(s) 700, 1270, 1288
Bse1I ACTGG 1 cut(s) 970
Bse21I CCTNAGG 2 cut(s) 369, 1353
Bse3DI GCAATG 4 cut(s) 142, 1221, 1407, 1582
BseBI CCWGG 5 cut(s) 522, 680, 1164, 1191, 1365
BseDI CCNNGG 5 cut(s) 708, 1057, 1190, 1324, 1339
BseGI GGATG 4 cut(s) 47, 513, 673, 1200
BseLI CCNNNNNNNGG 9 cut(s) 114, 374, 884, 1016, 1019, 1138, 1270, 1523, 1526
BseMI GCAATG 4 cut(s) 142, 1221, 1407, 1582
BseMII CTCAG 3 cut(s) 413, 896, 1344
BseNI ACTGG 1 cut(s) 970
BseRI GAGGAG 5 cut(s) 357, 360, 363, 879, 1506
BseSI GKGCMC 1 cut(s) 1033
BseX3I CGGCCG 1 cut(s) 1272
BseYI CCCAGC 2 cut(s) 1346, 1497
BsgI GTGCAG 1 cut(s) 207
Bsh1236I CGCG 2 cut(s) 170, 575
Bsh1285I CGRYCG 1 cut(s) 1275
BsiEI CGRYCG 1 cut(s) 1275
BsiHKAI GWGCWC 1 cut(s) 195
BsiSI CCGG 6 cut(s) 132, 339, 701, 1271, 1289, 1326
BslFI GGGAC 1 cut(s) 1448
BslI CCNNNNNNNGG 9 cut(s) 114, 374, 884, 1016, 1019, 1138, 1270, 1523, 1526
BsmAI GTCTC 2 cut(s) 885, 1141
BsmBI CGTCTC 1 cut(s) 885
BsmFI GGGAC 1 cut(s) 1448
Bso31I GGTCTC 1 cut(s) 1141
Bsp1286I GDGCHC 2 cut(s) 195, 1033
Bsp1407I TGTACA 1 cut(s) 89
Bsp143I GATC 6 cut(s) 123, 156, 481, 616, 670, 946
Bsp19I CCATGG 1 cut(s) 708
Bsp68I TCGCGA 1 cut(s) 170
BspCNI CTCAG 3 cut(s) 412, 895, 1345
BspFNI CGCG 2 cut(s) 170, 575
BspLI GGNNCC 3 cut(s) 337, 1221, 1324
BspMAI CTGCAG 2 cut(s) 447, 453
BspPI GGATC 2 cut(s) 476, 665
BspTNI GGTCTC 1 cut(s) 1141
BsrBI CCGCTC 1 cut(s) 1305
BsrDI GCAATG 4 cut(s) 142, 1221, 1407, 1582
BsrFI RCCGGY 3 cut(s) 700, 1270, 1288
BsrGI TGTACA 1 cut(s) 89
BsrI ACTGG 1 cut(s) 970
BssAI RCCGGY 3 cut(s) 700, 1270, 1288
BssECI CCNNGG 5 cut(s) 708, 1057, 1190, 1324, 1339
BssMI GATC 6 cut(s) 123, 156, 481, 616, 670, 946
BssT1I CCWWGG 3 cut(s) 708, 1057, 1339
Bst2UI CCWGG 5 cut(s) 522, 680, 1164, 1191, 1365
Bst4CI ACNGT 3 cut(s) 35, 998, 1389
BstAPI GCANNNNNTGC 4 cut(s) 415, 427, 442, 1186
BstAUI TGTACA 1 cut(s) 89
BstC8I GCNNGC 6 cut(s) 47, 168, 702, 1160, 1244, 1272
BstDEI CTNAG 4 cut(s) 369, 399, 882, 1353
BstDSI CCRYGG 1 cut(s) 708
BstEII GGTNACC 2 cut(s) 1367, 1529
BstF5I GGATG 4 cut(s) 47, 513, 673, 1200
BstFNI CGCG 2 cut(s) 170, 575
BstH2I RGCGCY 1 cut(s) 204
BstHHI GCGC 2 cut(s) 203, 877
BstKTI GATC 6 cut(s) 126, 159, 484, 619, 673, 949
BstMAI GTCTC 2 cut(s) 885, 1141
BstMBI GATC 6 cut(s) 123, 156, 481, 616, 670, 946
BstMCI CGRYCG 1 cut(s) 1275
BstNI CCWGG 5 cut(s) 522, 680, 1164, 1191, 1365
BstNSI RCATGY 4 cut(s) 262, 1162, 1246, 1492
BstPI GGTNACC 2 cut(s) 1367, 1529
BstSCI CCNGG 6 cut(s) 520, 678, 1162, 1189, 1324, 1363
BstSFI CTRYAG 2 cut(s) 443, 449
BstSLI GKGCMC 1 cut(s) 1033
BstUI CGCG 2 cut(s) 170, 575
BstX2I RGATCY 1 cut(s) 481
BstXI CCANNNNNNTGG 2 cut(s) 1064, 1346
BstYI RGATCY 1 cut(s) 481
BstZI CGGCCG 1 cut(s) 1272
Bsu36I CCTNAGG 2 cut(s) 369, 1353
BtgI CCRYGG 1 cut(s) 708
BtsCI GGATG 4 cut(s) 47, 513, 673, 1200
BtsIMutI CAGTG 2 cut(s) 31, 1558
BtuMI TCGCGA 1 cut(s) 170
Cac8I GCNNGC 6 cut(s) 47, 168, 702, 1160, 1244, 1272
CaiI CAGNNNCTG 1 cut(s) 733
CfoI GCGC 2 cut(s) 203, 877
Cfr10I RCCGGY 3 cut(s) 700, 1270, 1288
Cfr13I GGNCC 6 cut(s) 389, 1220, 1229, 1261, 1322, 1349
Csp6I GTAC 2 cut(s) 12, 90
CspCI CAANNNNNGTGG 2 cut(s) 979, 1014
CviQI GTAC 2 cut(s) 12, 90
DdeI CTNAG 4 cut(s) 369, 399, 882, 1353
DpnI GATC 6 cut(s) 125, 158, 483, 618, 672, 948
DpnII GATC 6 cut(s) 123, 156, 481, 616, 670, 946
DraIII CACNNNGTG 1 cut(s) 55
EaeI YGGCCR 2 cut(s) 243, 1272
EagI CGGCCG 1 cut(s) 1272
EciI GGCGGA 2 cut(s) 342, 876
Ecl136II GAGCTC 1 cut(s) 193
EclXI CGGCCG 1 cut(s) 1272
Eco130I CCWWGG 3 cut(s) 708, 1057, 1339
Eco147I AGGCCT 1 cut(s) 534
Eco24I GRGCYC 1 cut(s) 195
Eco31I GGTCTC 1 cut(s) 1141
Eco47I GGWCC 2 cut(s) 1220, 1229
Eco52I CGGCCG 1 cut(s) 1272
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 2 cut(s) 443, 1534
Eco81I CCTNAGG 2 cut(s) 369, 1353
Eco91I GGTNACC 2 cut(s) 1367, 1529
EcoICRI GAGCTC 1 cut(s) 193
EcoO65I GGTNACC 2 cut(s) 1367, 1529
EcoRII CCWGG 5 cut(s) 520, 678, 1162, 1189, 1363
EcoT14I CCWWGG 3 cut(s) 708, 1057, 1339
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 3 cut(s) 708, 1057, 1339
Esp3I CGTCTC 1 cut(s) 885
FalI AAGNNNNNCTT 2 cut(s) 50, 82
FaqI GGGAC 1 cut(s) 1448
FauI CCCGC 1 cut(s) 1424
FbaI TGATCA 1 cut(s) 156
FblI GTMKAC 1 cut(s) 1285
FokI GGATG 4 cut(s) 54, 520, 680, 1207
FriOI GRGCYC 1 cut(s) 195
FspBI CTAG 1 cut(s) 546
GlaI GCGC 2 cut(s) 202, 876
GsaI CCCAGC 2 cut(s) 1350, 1501
GsuI CTGGAG 2 cut(s) 1185, 1503
HaeII RGCGCY 1 cut(s) 204
HapII CCGG 6 cut(s) 132, 339, 701, 1271, 1289, 1326
HhaI GCGC 2 cut(s) 203, 877
Hin6I GCGC 2 cut(s) 201, 875
HinP1I GCGC 2 cut(s) 201, 875
HincII GTYRAC 2 cut(s) 25, 142
HindII GTYRAC 2 cut(s) 25, 142
HinfI GANTC 2 cut(s) 104, 1468
HpaI GTTAAC 1 cut(s) 25
HpaII CCGG 6 cut(s) 132, 339, 701, 1271, 1289, 1326
HphI GGTGA 3 cut(s) 67, 133, 1541
Hpy166II GTNNAC 4 cut(s) 25, 142, 800, 1286
Hpy188I TCNGA 9 cut(s) 109, 156, 207, 228, 252, 486, 687, 786, 1257
Hpy188III TCNNGA 3 cut(s) 160, 169, 620
Hpy8I GTNNAC 4 cut(s) 25, 142, 800, 1286
Hpy99I CGWCG 1 cut(s) 647
HpyAV CCTTC 7 cut(s) 74, 112, 202, 304, 463, 553, 781
HpyCH4III ACNGT 3 cut(s) 35, 998, 1389
HpyCH4IV ACGT 1 cut(s) 184
HpyF3I CTNAG 4 cut(s) 369, 399, 882, 1353
HpySE526I ACGT 1 cut(s) 184
HspAI GCGC 2 cut(s) 201, 875
KroI GCCGGC 2 cut(s) 700, 1270
KroNI GCCGGC 2 cut(s) 702, 1272
Ksp22I TGATCA 1 cut(s) 156
KspAI GTTAAC 1 cut(s) 25
Kzo9I GATC 6 cut(s) 123, 156, 481, 616, 670, 946
LmnI GCTCC 4 cut(s) 208, 335, 1067, 1343
LweI GCATC 4 cut(s) 32, 498, 735, 1176
MaeI CTAG 1 cut(s) 546
MaeII ACGT 1 cut(s) 184
MaeIII GTNAC 3 cut(s) 1046, 1367, 1529
MalI GATC 6 cut(s) 125, 158, 483, 618, 672, 948
MbiI CCGCTC 1 cut(s) 1305
MboI GATC 6 cut(s) 123, 156, 481, 616, 670, 946
MboII GAAGA 4 cut(s) 70, 265, 644, 1540
MfeI CAATTG 1 cut(s) 497
MflI RGATCY 1 cut(s) 481
MhlI GDGCHC 2 cut(s) 195, 1033
MlsI TGGCCA 1 cut(s) 245
MluCI AATT 3 cut(s) 75, 497, 569
MluNI TGGCCA 1 cut(s) 245
Mox20I TGGCCA 1 cut(s) 245
MroNI GCCGGC 2 cut(s) 700, 1270
MscI TGGCCA 1 cut(s) 245
MseI TTAA 2 cut(s) 24, 1391
MslI CAYNNNNRTG 3 cut(s) 36, 1214, 1247
Msp20I TGGCCA 1 cut(s) 245
MspA1I CMGCKG 2 cut(s) 448, 1523
MspI CCGG 6 cut(s) 132, 339, 701, 1271, 1289, 1326
MspR9I CCNGG 6 cut(s) 522, 680, 1164, 1191, 1326, 1365
MunI CAATTG 1 cut(s) 497
MvaI CCWGG 5 cut(s) 522, 680, 1164, 1191, 1365
MvnI CGCG 2 cut(s) 170, 575
NaeI GCCGGC 2 cut(s) 702, 1272
NciI CCSGG 1 cut(s) 1326
NcoI CCATGG 1 cut(s) 708
NdeII GATC 6 cut(s) 123, 156, 481, 616, 670, 946
NgoMIV GCCGGC 2 cut(s) 700, 1270
NlaIV GGNNCC 3 cut(s) 337, 1221, 1324
NmuCI GTSAC 1 cut(s) 1529
NruI TCGCGA 1 cut(s) 170
NspI RCATGY 4 cut(s) 262, 1162, 1246, 1492
PaeI GCATGC 2 cut(s) 1162, 1246
PceI AGGCCT 1 cut(s) 534
PdiI GCCGGC 2 cut(s) 702, 1272
PfeI GAWTC 2 cut(s) 104, 1468
PflMI CCANNNNNTGG 3 cut(s) 1019, 1138, 1526
Psp124BI GAGCTC 1 cut(s) 195
Psp6I CCWGG 5 cut(s) 520, 678, 1162, 1189, 1363
PspEI GGTNACC 2 cut(s) 1367, 1529
PspFI CCCAGC 2 cut(s) 1346, 1497
PspGI CCWGG 5 cut(s) 520, 678, 1162, 1189, 1363
PspN4I GGNNCC 3 cut(s) 337, 1221, 1324
PspPI GGNCC 6 cut(s) 389, 1220, 1229, 1261, 1322, 1349
PstI CTGCAG 2 cut(s) 447, 453
PstNI CAGNNNCTG 1 cut(s) 733
PsuI RGATCY 1 cut(s) 481
PvuII CAGCTG 2 cut(s) 448, 1523
RruI TCGCGA 1 cut(s) 170
RsaI GTAC 2 cut(s) 13, 91
RsaNI GTAC 2 cut(s) 12, 90
RseI CAYNNNNRTG 3 cut(s) 36, 1214, 1247
SacI GAGCTC 1 cut(s) 195
SaqAI TTAA 2 cut(s) 24, 1391
Sau3AI GATC 6 cut(s) 123, 156, 481, 616, 670, 946
Sau96I GGNCC 6 cut(s) 389, 1220, 1229, 1261, 1322, 1349
ScrFI CCNGG 6 cut(s) 522, 680, 1164, 1191, 1326, 1365
SduI GDGCHC 2 cut(s) 195, 1033
SfaNI GCATC 4 cut(s) 32, 498, 735, 1176
SfcI CTRYAG 2 cut(s) 443, 449
SinI GGWCC 2 cut(s) 1220, 1229
SmiMI CAYNNNNRTG 3 cut(s) 36, 1214, 1247
SmlI CTYRAG 1 cut(s) 1152
SmoI CTYRAG 1 cut(s) 1152
SphI GCATGC 2 cut(s) 1162, 1246
Sse9I AATT 3 cut(s) 75, 497, 569
SseBI AGGCCT 1 cut(s) 534
SspMI CTAG 1 cut(s) 546
SstI GAGCTC 1 cut(s) 195
StuI AGGCCT 1 cut(s) 534
StyD4I CCNGG 6 cut(s) 520, 678, 1162, 1189, 1324, 1363
StyI CCWWGG 3 cut(s) 708, 1057, 1339
TaaI ACNGT 3 cut(s) 35, 998, 1389
TaiI ACGT 1 cut(s) 187
TaqI TCGA 1 cut(s) 1478
TasI AATT 3 cut(s) 75, 497, 569
TatI WGTACW 2 cut(s) 11, 89
TfiI GAWTC 2 cut(s) 104, 1468
Tru1I TTAA 2 cut(s) 24, 1391
Tru9I TTAA 2 cut(s) 24, 1391
TscAI CASTG 2 cut(s) 38, 1558
TseFI GTSAC 1 cut(s) 1529
Tsp45I GTSAC 1 cut(s) 1529
TspDTI ATGAA 7 cut(s) 482, 710, 773, 791, 1103, 1220, 1421
TspGWI ACGGA 2 cut(s) 139, 1065
TspRI CASTG 2 cut(s) 38, 1558
Van91I CCANNNNNTGG 3 cut(s) 1019, 1138, 1526
VpaK11BI GGWCC 2 cut(s) 1220, 1229
XapI RAATTY 1 cut(s) 569
XceI RCATGY 4 cut(s) 262, 1162, 1246, 1492
XcmI CCANNNNNNNNNTGG 2 cut(s) 314, 1139
XmiI GTMKAC 1 cut(s) 1285
XspI CTAG 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.