Rh4BG216800

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0007097
Physical Location & Seq
Forward (+)
37732026 .. 37735037
3012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG216800.1

Sequence Viewer

Length: 1590 bp
ATGCAGAAATGTACTCTCAAAGTTAACATTCACTGTGATGGATGCAAGCACAAGGTGAAGAAAATCTTGCAGAGAATTGAAGGGGTTTGTACAACCGACATAGATTCCGAGCAGGGGAAGGTGATCGTTTCCGGCAATGTTGACCCCTCCGTTCTGATCAAGAAGCTCGCGAAATCAGGGAAACGTGCAGAGCTCTGGGGCGCTCCGAAGGCCAACAACAACAATCAGAACCAGAACAACATGGCCAATCAGAAGAACATGCAACTTGACAATGGCAAAGGTGGGAATAACAAAGGCCAAAACAACCAGAAGGGCGGTGGCGGTGGCGGAGCCGGAGCCGGAGGAGGAGGAGGCAACAACAATCAGCCTAAGGGTGGAGGACAACAAGGCCCAAACCCTCAGCAACAGCAACAAATGCAACAGCAAATGCAGCAGCAACTCCTGCAGCTGCAGCAGCTTCAGCAAATGAAGGGGTTGCAAGATCTGAAGCTGCCCCAATTGAAAGCGATGCAAATGCCTCCTGCTTTCAAAGGGCCGAACCCACCTGGCCAGAACCAGAAGGCTGTCAAATTCGCGGACATGGACGATGATGACATGACTGATGATGAGTATGATGATCTTGATGATGACGAAGATTATGACGACGAGGATTTTGATGACGAGCTGGATGATCCTCGCCTGGCTCTGAATAAGATGAAGCCGGCGGCAATAGGTGGTGGTGGTGGTCAGGGGCTGCCTCCTATGATGATGAATGGAATGAACCAGCCACAGATGATGATGAATGTTCAGAAGGCTGGTGGTGGACAACCGAATGGTGGCGGCGGCGGTGGTGGTAATGGTGGAAAGAAAGGTGGTGGTGGCGGCGGAGGAGGGGGTGCGCCGTCTCAGGGGAATATGGGCGGTGGCGGTGGCGGTGGCGGTGGAAAGAAAGGCGGTGGCGGTGGTGGAGATCATGGTCAAAATCAAGCTGGGGGTGGCGGCAAAAATGTAGGCAAGAACGGTGGTGGGTTTCCCCCAAATGGTGGTGGTGGGGCACAGAACAAGAATGGTAACGGAGGCCAAGGTGGTGGAGCTAAAAAGGGTGGTGGTGGTGGTGGAATGAGTGAGGCTCAACTTCATGCTATGATGAGCCACCAGAACATGGGTGGGAGACCTCAAGGCATGCCTGGAGGTAATGTGGGGCAGCAGATGCCTGGGATGAGCCAGATGAACATGGCAATGGGTCCAATGGGTCCAATGGGAAGCATGCCAATGGGTCAGATGGGCCAAATGCCGGCCGTTCAAGGTCTACCGGCTGCCACCATGAGCGGCGGCGGCGGCGGCGGCCCCGGTGGCTACTTCCAAGGAGCTGGGCCTGAGGCCATGCCTGGTAACCCATACCAGAACCCACAGTTAATGGCTGCAATGATGAACCAGCAGAGAGCTATGGGCGGCGGGGACAGGTCGTTCCAGCCTATGATGTATAATAGGATTCCTCCATCGACCAACTACATGCCGCCCCAGCATTACCCATACCCTCCTCCAGCTGGTGGTGACCACTACACCCATTTCTTCAGTGATGAGAACACTTCAAGCTGCAATGTGATGTGA

Protein Analysis

529

Amino Acids

53.82

Weight (kDa)

8.11

Isoelectric Point (pI)

58.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 5 - 61 1.8e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 1022, 1141, 1529
AccBSI CCGCTC 1 cut(s) 1308
AccI GTMKAC 1 cut(s) 1288
AccII CGCG 2 cut(s) 170, 575
AclWI GGATC 1 cut(s) 665
AcoI YGGCCR 3 cut(s) 243, 547, 1275
AcsI RAATTY 1 cut(s) 569
AcuI CTGAAG 3 cut(s) 443, 506, 1537
AdeI CACNNNGTG 1 cut(s) 55
AfaI GTAC 2 cut(s) 13, 91
AgsI TTSAA 5 cut(s) 80, 502, 529, 1283, 1572
AjnI CCWGG 5 cut(s) 544, 678, 1165, 1192, 1366
AloI GAACNNNNNNTCC 2 cut(s) 1430, 1462
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 2 cut(s) 888, 1144
AlwI GGATC 1 cut(s) 665
AlwNI CAGNNNCTG 1 cut(s) 733
ApoI RAATTY 1 cut(s) 569
ArsI GACNNNNNNTTYG 2 cut(s) 635, 667
AspLEI GCGC 2 cut(s) 203, 880
AspS9I GGNCC 7 cut(s) 389, 533, 1223, 1232, 1264, 1325, 1352
AsuC2I CCSGG 1 cut(s) 1329
AsuHPI GGTGA 3 cut(s) 67, 133, 1544
AvaII GGWCC 2 cut(s) 1223, 1232
AxyI CCTNAGG 2 cut(s) 369, 1356
BaeGI GKGCMC 1 cut(s) 1036
BalI TGGCCA 2 cut(s) 245, 549
BanII GRGCYC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 195
BbvCI CCTCAGC 1 cut(s) 399
BccI CCATC 3 cut(s) 32, 1255, 1486
BceAI ACGGC 2 cut(s) 865, 1262
BcgI CGANNNNNNTGC 2 cut(s) 496, 530
BciT130I CCWGG 5 cut(s) 546, 680, 1167, 1194, 1368
BclI TGATCA 1 cut(s) 156
BcnI CCSGG 1 cut(s) 1329
BcoDI GTCTC 2 cut(s) 888, 1144
BfmI CTRYAG 2 cut(s) 443, 449
BfoI RGCGCY 1 cut(s) 204
BglI GCCNNNNNGGC 1 cut(s) 1332
BglII AGATCT 1 cut(s) 481
Bme1390I CCNGG 6 cut(s) 546, 680, 1167, 1194, 1329, 1368
Bme18I GGWCC 2 cut(s) 1223, 1232
BmgT120I GGNCC 7 cut(s) 389, 533, 1223, 1232, 1264, 1325, 1352
BmiI GGNNCC 5 cut(s) 331, 337, 1224, 1233, 1327
BmrFI CCNGG 6 cut(s) 546, 680, 1167, 1194, 1329, 1368
BmsI GCATC 3 cut(s) 32, 498, 1179
BplI GAGNNNNNCTC 2 cut(s) 1093, 1125
BpmI CTGGAG 2 cut(s) 1188, 1506
Bpu10I CCTNAGC 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 1140
BpuMI CCSGG 1 cut(s) 1329
BsaI GGTCTC 1 cut(s) 1144
BsaJI CCNNGG 4 cut(s) 1060, 1193, 1327, 1342
BsaXI ACNNNNNCTCC 2 cut(s) 1161, 1191
Bse118I RCCGGY 3 cut(s) 700, 1273, 1291
Bse21I CCTNAGG 2 cut(s) 369, 1356
Bse3DI GCAATG 4 cut(s) 142, 1224, 1410, 1585
BseBI CCWGG 5 cut(s) 546, 680, 1167, 1194, 1368
BseDI CCNNGG 4 cut(s) 1060, 1193, 1327, 1342
BseGI GGATG 3 cut(s) 47, 673, 1203
BseMI GCAATG 4 cut(s) 142, 1224, 1410, 1585
BseMII CTCAG 3 cut(s) 413, 899, 1347
BseRI GAGGAG 5 cut(s) 357, 360, 363, 882, 1509
BseSI GKGCMC 1 cut(s) 1036
BseX3I CGGCCG 1 cut(s) 1275
BseYI CCCAGC 3 cut(s) 968, 1349, 1500
BsgI GTGCAG 1 cut(s) 207
Bsh1236I CGCG 2 cut(s) 170, 575
Bsh1285I CGRYCG 1 cut(s) 1278
BsiEI CGRYCG 1 cut(s) 1278
BsiHKAI GWGCWC 1 cut(s) 195
BsiSI CCGG 7 cut(s) 132, 333, 339, 701, 1274, 1292, 1329
BslFI GGGAC 1 cut(s) 1451
BsmAI GTCTC 2 cut(s) 888, 1144
BsmBI CGTCTC 1 cut(s) 888
BsmFI GGGAC 1 cut(s) 1451
Bso31I GGTCTC 1 cut(s) 1144
Bsp1286I GDGCHC 2 cut(s) 195, 1036
Bsp1407I TGTACA 1 cut(s) 89
Bsp143I GATC 6 cut(s) 123, 156, 481, 616, 670, 949
Bsp68I TCGCGA 1 cut(s) 170
BspCNI CTCAG 3 cut(s) 412, 898, 1348
BspFNI CGCG 2 cut(s) 170, 575
BspLI GGNNCC 5 cut(s) 331, 337, 1224, 1233, 1327
BspMAI CTGCAG 2 cut(s) 447, 453
BspPI GGATC 1 cut(s) 665
BspTNI GGTCTC 1 cut(s) 1144
BsrBI CCGCTC 1 cut(s) 1308
BsrDI GCAATG 4 cut(s) 142, 1224, 1410, 1585
BsrFI RCCGGY 3 cut(s) 700, 1273, 1291
BsrGI TGTACA 1 cut(s) 89
BssAI RCCGGY 3 cut(s) 700, 1273, 1291
BssECI CCNNGG 4 cut(s) 1060, 1193, 1327, 1342
BssMI GATC 6 cut(s) 123, 156, 481, 616, 670, 949
BssT1I CCWWGG 2 cut(s) 1060, 1342
Bst2UI CCWGG 5 cut(s) 546, 680, 1167, 1194, 1368
Bst4CI ACNGT 3 cut(s) 35, 1001, 1392
BstAPI GCANNNNNTGC 3 cut(s) 415, 442, 1189
BstAUI TGTACA 1 cut(s) 89
BstC8I GCNNGC 6 cut(s) 47, 168, 702, 1163, 1247, 1275
BstDEI CTNAG 4 cut(s) 369, 399, 885, 1356
BstEII GGTNACC 2 cut(s) 1370, 1532
BstF5I GGATG 3 cut(s) 47, 673, 1203
BstFNI CGCG 2 cut(s) 170, 575
BstH2I RGCGCY 1 cut(s) 204
BstHHI GCGC 2 cut(s) 203, 880
BstKTI GATC 6 cut(s) 126, 159, 484, 619, 673, 952
BstMAI GTCTC 2 cut(s) 888, 1144
BstMBI GATC 6 cut(s) 123, 156, 481, 616, 670, 949
BstMCI CGRYCG 1 cut(s) 1278
BstNI CCWGG 5 cut(s) 546, 680, 1167, 1194, 1368
BstNSI RCATGY 4 cut(s) 262, 1165, 1249, 1495
BstPI GGTNACC 2 cut(s) 1370, 1532
BstSCI CCNGG 6 cut(s) 544, 678, 1165, 1192, 1327, 1366
BstSFI CTRYAG 2 cut(s) 443, 449
BstSLI GKGCMC 1 cut(s) 1036
BstUI CGCG 2 cut(s) 170, 575
BstX2I RGATCY 1 cut(s) 481
BstXI CCANNNNNNTGG 2 cut(s) 1067, 1349
BstYI RGATCY 1 cut(s) 481
BstZI CGGCCG 1 cut(s) 1275
Bsu36I CCTNAGG 2 cut(s) 369, 1356
BtgZI GCGATG 1 cut(s) 521
BtsCI GGATG 3 cut(s) 47, 673, 1203
BtsIMutI CAGTG 2 cut(s) 31, 1561
BtuMI TCGCGA 1 cut(s) 170
Cac8I GCNNGC 6 cut(s) 47, 168, 702, 1163, 1247, 1275
CaiI CAGNNNCTG 1 cut(s) 733
CfoI GCGC 2 cut(s) 203, 880
Cfr10I RCCGGY 3 cut(s) 700, 1273, 1291
Cfr13I GGNCC 7 cut(s) 389, 533, 1223, 1232, 1264, 1325, 1352
Csp6I GTAC 2 cut(s) 12, 90
CspCI CAANNNNNGTGG 2 cut(s) 982, 1017
CviQI GTAC 2 cut(s) 12, 90
DdeI CTNAG 4 cut(s) 369, 399, 885, 1356
DpnI GATC 6 cut(s) 125, 158, 483, 618, 672, 951
DpnII GATC 6 cut(s) 123, 156, 481, 616, 670, 949
DraIII CACNNNGTG 1 cut(s) 55
EaeI YGGCCR 3 cut(s) 243, 547, 1275
EagI CGGCCG 1 cut(s) 1275
EciI GGCGGA 2 cut(s) 342, 879
Ecl136II GAGCTC 1 cut(s) 193
EclXI CGGCCG 1 cut(s) 1275
Eco130I CCWWGG 2 cut(s) 1060, 1342
Eco24I GRGCYC 1 cut(s) 195
Eco31I GGTCTC 1 cut(s) 1144
Eco47I GGWCC 2 cut(s) 1223, 1232
Eco52I CGGCCG 1 cut(s) 1275
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 3 cut(s) 443, 506, 1537
Eco81I CCTNAGG 2 cut(s) 369, 1356
Eco91I GGTNACC 2 cut(s) 1370, 1532
EcoICRI GAGCTC 1 cut(s) 193
EcoO65I GGTNACC 2 cut(s) 1370, 1532
EcoRII CCWGG 5 cut(s) 544, 678, 1165, 1192, 1366
EcoT14I CCWWGG 2 cut(s) 1060, 1342
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 2 cut(s) 1060, 1342
Esp3I CGTCTC 1 cut(s) 888
FalI AAGNNNNNCTT 2 cut(s) 50, 82
FaqI GGGAC 1 cut(s) 1451
FauI CCCGC 1 cut(s) 1427
FbaI TGATCA 1 cut(s) 156
FblI GTMKAC 1 cut(s) 1288
FokI GGATG 3 cut(s) 54, 680, 1210
FriOI GRGCYC 1 cut(s) 195
GlaI GCGC 2 cut(s) 202, 879
GsaI CCCAGC 3 cut(s) 972, 1353, 1504
GsuI CTGGAG 2 cut(s) 1188, 1506
HaeII RGCGCY 1 cut(s) 204
HapII CCGG 7 cut(s) 132, 333, 339, 701, 1274, 1292, 1329
HhaI GCGC 2 cut(s) 203, 880
Hin6I GCGC 2 cut(s) 201, 878
HinP1I GCGC 2 cut(s) 201, 878
HincII GTYRAC 2 cut(s) 25, 142
HindII GTYRAC 2 cut(s) 25, 142
HinfI GANTC 2 cut(s) 104, 1471
HpaI GTTAAC 1 cut(s) 25
HpaII CCGG 7 cut(s) 132, 333, 339, 701, 1274, 1292, 1329
HphI GGTGA 3 cut(s) 67, 133, 1544
Hpy166II GTNNAC 4 cut(s) 25, 142, 803, 1289
Hpy188I TCNGA 9 cut(s) 109, 156, 207, 228, 252, 486, 687, 789, 1260
Hpy188III TCNNGA 3 cut(s) 160, 169, 620
Hpy8I GTNNAC 4 cut(s) 25, 142, 803, 1289
Hpy99I CGWCG 1 cut(s) 647
HpyAV CCTTC 7 cut(s) 74, 112, 202, 304, 463, 553, 784
HpyCH4III ACNGT 3 cut(s) 35, 1001, 1392
HpyCH4IV ACGT 1 cut(s) 184
HpyF3I CTNAG 4 cut(s) 369, 399, 885, 1356
HpySE526I ACGT 1 cut(s) 184
HspAI GCGC 2 cut(s) 201, 878
KroI GCCGGC 2 cut(s) 700, 1273
KroNI GCCGGC 2 cut(s) 702, 1275
Ksp22I TGATCA 1 cut(s) 156
KspAI GTTAAC 1 cut(s) 25
Kzo9I GATC 6 cut(s) 123, 156, 481, 616, 670, 949
LmnI GCTCC 5 cut(s) 208, 329, 335, 1070, 1346
LweI GCATC 3 cut(s) 32, 498, 1179
MaeII ACGT 1 cut(s) 184
MaeIII GTNAC 3 cut(s) 1049, 1370, 1532
MalI GATC 6 cut(s) 125, 158, 483, 618, 672, 951
MbiI CCGCTC 1 cut(s) 1308
MboI GATC 6 cut(s) 123, 156, 481, 616, 670, 949
MboII GAAGA 4 cut(s) 70, 265, 644, 1543
MfeI CAATTG 1 cut(s) 497
MflI RGATCY 1 cut(s) 481
MhlI GDGCHC 2 cut(s) 195, 1036
MlsI TGGCCA 2 cut(s) 245, 549
MluCI AATT 3 cut(s) 75, 497, 569
MluNI TGGCCA 2 cut(s) 245, 549
Mox20I TGGCCA 2 cut(s) 245, 549
MroNI GCCGGC 2 cut(s) 700, 1273
MscI TGGCCA 2 cut(s) 245, 549
MseI TTAA 2 cut(s) 24, 1394
MslI CAYNNNNRTG 3 cut(s) 36, 1217, 1250
Msp20I TGGCCA 2 cut(s) 245, 549
MspA1I CMGCKG 2 cut(s) 448, 1526
MspI CCGG 7 cut(s) 132, 333, 339, 701, 1274, 1292, 1329
MspR9I CCNGG 6 cut(s) 546, 680, 1167, 1194, 1329, 1368
MunI CAATTG 1 cut(s) 497
MvaI CCWGG 5 cut(s) 546, 680, 1167, 1194, 1368
MvnI CGCG 2 cut(s) 170, 575
NaeI GCCGGC 2 cut(s) 702, 1275
NciI CCSGG 1 cut(s) 1329
NdeII GATC 6 cut(s) 123, 156, 481, 616, 670, 949
NgoMIV GCCGGC 2 cut(s) 700, 1273
NlaIV GGNNCC 5 cut(s) 331, 337, 1224, 1233, 1327
NmuCI GTSAC 1 cut(s) 1532
NruI TCGCGA 1 cut(s) 170
NspI RCATGY 4 cut(s) 262, 1165, 1249, 1495
PaeI GCATGC 2 cut(s) 1165, 1249
PdiI GCCGGC 2 cut(s) 702, 1275
PfeI GAWTC 2 cut(s) 104, 1471
PflMI CCANNNNNTGG 3 cut(s) 1022, 1141, 1529
Psp124BI GAGCTC 1 cut(s) 195
Psp6I CCWGG 5 cut(s) 544, 678, 1165, 1192, 1366
PspEI GGTNACC 2 cut(s) 1370, 1532
PspFI CCCAGC 3 cut(s) 968, 1349, 1500
PspGI CCWGG 5 cut(s) 544, 678, 1165, 1192, 1366
PspN4I GGNNCC 5 cut(s) 331, 337, 1224, 1233, 1327
PspPI GGNCC 7 cut(s) 389, 533, 1223, 1232, 1264, 1325, 1352
PstI CTGCAG 2 cut(s) 447, 453
PstNI CAGNNNCTG 1 cut(s) 733
PsuI RGATCY 1 cut(s) 481
PvuII CAGCTG 2 cut(s) 448, 1526
RruI TCGCGA 1 cut(s) 170
RsaI GTAC 2 cut(s) 13, 91
RsaNI GTAC 2 cut(s) 12, 90
RseI CAYNNNNRTG 3 cut(s) 36, 1217, 1250
SacI GAGCTC 1 cut(s) 195
SaqAI TTAA 2 cut(s) 24, 1394
Sau3AI GATC 6 cut(s) 123, 156, 481, 616, 670, 949
Sau96I GGNCC 7 cut(s) 389, 533, 1223, 1232, 1264, 1325, 1352
ScrFI CCNGG 6 cut(s) 546, 680, 1167, 1194, 1329, 1368
SduI GDGCHC 2 cut(s) 195, 1036
SfaNI GCATC 3 cut(s) 32, 498, 1179
SfcI CTRYAG 2 cut(s) 443, 449
SinI GGWCC 2 cut(s) 1223, 1232
SmiMI CAYNNNNRTG 3 cut(s) 36, 1217, 1250
SmlI CTYRAG 1 cut(s) 1155
SmoI CTYRAG 1 cut(s) 1155
SphI GCATGC 2 cut(s) 1165, 1249
Sse9I AATT 3 cut(s) 75, 497, 569
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 6 cut(s) 544, 678, 1165, 1192, 1327, 1366
StyI CCWWGG 2 cut(s) 1060, 1342
TaaI ACNGT 3 cut(s) 35, 1001, 1392
TaiI ACGT 1 cut(s) 187
TaqI TCGA 1 cut(s) 1481
TasI AATT 3 cut(s) 75, 497, 569
TatI WGTACW 2 cut(s) 11, 89
TfiI GAWTC 2 cut(s) 104, 1471
Tru1I TTAA 2 cut(s) 24, 1394
Tru9I TTAA 2 cut(s) 24, 1394
TscAI CASTG 2 cut(s) 38, 1561
TseFI GTSAC 1 cut(s) 1532
Tsp45I GTSAC 1 cut(s) 1532
TspDTI ATGAA 8 cut(s) 482, 710, 764, 773, 794, 1106, 1223, 1424
TspGWI ACGGA 2 cut(s) 139, 1068
TspRI CASTG 2 cut(s) 38, 1561
Van91I CCANNNNNTGG 3 cut(s) 1022, 1141, 1529
VpaK11BI GGWCC 2 cut(s) 1223, 1232
XapI RAATTY 1 cut(s) 569
XceI RCATGY 4 cut(s) 262, 1165, 1249, 1495
XcmI CCANNNNNNNNNTGG 2 cut(s) 314, 1142
XmiI GTMKAC 1 cut(s) 1288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.