pycom13g16500

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007814
Physical Location & Seq
Forward (+)
12325416 .. 12331571
6156 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g16500.6

Sequence Viewer

Length: 3732 bp
ATGGACGAAGGTGTGAGATCTGTTGGCCCTTCTGGGGTTTTGGTGAAGAAAAATTCTTCTGGTTGTTTAATTGTGAGGAAGAAACCCGAGGGGTTGAGTGGTGCTGTTGGTTCTTCGAGTGCTCGGAAGGTTTTTGAACCCAAGGACAAGAAGAGGTCGAGGTTGGTCTTGAGCGATTCGGGGTCTAGTGATGAGATACCTCCTCGTAGAAAGGTGGAACCTGAAACTATACGGGTTTCCAATGGTTTAAGAGCTGTAGATAAGGGCATTGCTGAAGATAGTGAATTTAGTCAAAAGCGGGAGAGATTTGATCCGGTTAGGCGTAGCGAGGATGGCATGATTGGTAAAGGTTATTTGGATGAGAGTGGTGGAAAGAGAAGTAAGTTGGAGGTGTACGAATTCGATGAGTATGATGCAGAGATTTCGAGAAGGAAACGGTTTAATGATGGGGTGGGTGAGTTTGGGGGGAGAAGGTTTTCAGGAGCGATTCCTGTGCCACTAAGTGGCATTAAGAGGGAATTTGAAAGTGGTTCAAGAAGACATGTAGTTGACAAGAGGAAGAATTTGTACTTTGACAGGACGACTAGCTTGAACCGAGATCATACTGACAGGGGTAGGTTTGAGATGAATAGGGATGGAGCTCAGCTACCCTTATTGAGAGACAAGTTCACAGGTCAATCAGAGGAATCCATTAGGCTGCAGGGTAAAAATGGTGTTTTGAAGGTCATGGTGAAGAAGAAAAAGAATGCAGGTGGGCCACTGGAGAACTATAATTTTCGCAAATCTGAAGAAAATAGAAAGGCTCTGAGTGACAATATTGCGAAAAGTGCTATTATCCCTCCATTTTACTCTGAACCAAAGCTTATTGAGAAACCAGTTTCAGTGGTTAGGACAGTGAAAAACCATCCGAATGTGCGAAAATCATTGCCTTCTTTGAACAGTAAGGGCTCGGACTGGGATTCAGAGGATAGTGATACATCATTGAAACTAGGACCAAAGAGTGTGGAAGCCTCTAAACCTGTGAAGAGGGTGGTTTGCAAAGATGAAGGTGCTCCTTCATGTGAAAAGACTCCTCCATCCAGAATCAAAGAAGGTAAAGTTAAACGTGGTAGTGGCACGGAAAAGCAAAAACTACGTGAACGAATACGAGAGATGCTTCTGAGTGCAGGCTGGACAATAGATTATAGGCCTAGAAGGAACAGAGACTATCTAGATGCTGTGTACATTAACCCAGCAGGTACAGCTTACTGGTCCATCATTAAGGCTTATGATGCGCTTCAAAAGCAATTGAATGAGGAGGAAAATGAGGCTAAACCTAGTGGGGTTCAACCTATAACTGATGATGTACTCAGCCAATTAACAAGGAAAACTCGAAAGAAGATTGAGAAAGAAATGAAAAGGAAGCAAAGGGCTGATGCTGAGGGTGAAAATGCGAGAGGAGTCCGTATGAAAAGATCCGCAAGTATCAAGCGTGATCCAGAGAGCATGGATAGTGTTAGTTATGAGGAGAAACTAAGCACCTACTTAAAGCAGGGTGGTAAGTCGTTCAAGGGGAAAATGAGTGAAAATGGTTTGGCCAATGTGAACTCAAATCATCCACACGAAAGTATTGAAAAACCGTCTTCAGGATCAAGTTCGCATATGCCACATGAAAGGAAAAGTAGAAAGCTTGGAAGGTGCACTTTGTTGGTTCGTGGTTCTAATTATGGGGTCAACTCGGAAAGTGATGGCTTTGTTCCATATACTGGGAAACGGACGTTGCTTTCCTGGCTGATTGACTCAGGAACTGTTCAATTGAGCCAAAAGGTGCAGTATATGAATCGCCGAAGGACTAAAGTGATGCTGGAGGGGTGGATTACAAGAGATGGAATTCACTGCGGTTGCTGTAGTAAAATCCTCACAACTTCAAAATTTGAGATTCATGCAGGAAGCAAGTTGCGCCAGCCATTTCAAAATATATGTTTGGACTCTGGCGTTTCCCTTCTGCAGTGTCAATTAGATGCATGGAATAGACAAAAGGTTACTGAACGCATTGGCTTCCACTCCGTAGAAGTCGACGGTGACGATCCAGATGATGATACTTGTGGCATCTGTGGGGATGGCGAGGAATCGATCTGTTGCGATAGCTGTCCATCAACATTTCATCAGAGCTGCTTGAATATACGGATGCTTCCTCCAGGTCACATATCATGCTGTCAGGAGATGGATGGTAGTCCTGCTGATCCTCCTAGTTTAGACTCTTCATTCTGTGGGCAGAAATGCAGAGAGCTCTTTGAGAATTTGAAGAAGTATCTTGGGGTCAAACATGAACTAGAAGCAGGATTTTCATGGACACTTGTTCATAGAACTGATGAAGATGAAGGGTTCCCGCAGAGGATCGATCCCAATTCTAAGCTTGCTGTTGCTTTGACTGTTATGGATGAATGCTTTTTGCCTATTGTTGACAGGAGGAGCGGCATCAATTTAATTCATAATGTTCTTTATAATTGTGGATCGATCTTCAATCGGCTGAACTACAGTGGCTTCTACACTGCTATTTTAGAGAGAGGCGATGAAATAATTTCTGCTGCATCAATCAGGTTTCATGGTACAAAGTTAGCAGAGATGCCATACATAGGAACTCGCCACATATATAGGCGACAAGGGATGTGCCGCCGGCTTTTTTGTGCCATTGAATCGGCTCTGTGCTCTCTCAAGGTTGAGAAATTGGTTATCCCTGCAATTGCTGAACTCATGCATACATGGACAGAAGTTTTTGGTTTTATTCCTGTTGAGGAATCATTCAAGCAAGAAATGAGGTCAATGAATATGCTAGTATTCCCTGGTATAGACATGCTACAGAAGTTGCTAGTGGATCAAGGCAATGAGGGGAACGTGACAGCAGACCCAGGCTTAAAGCAATTAGAATGCAAAGGCAAAGATTTAATAAAGCATGGGAGTGGAAGCAAATCAGATAGTGGTTCACCTGCTAGTATTGATCCTCGTGGATCTGATGAAACCAGTTTGCCTCACATAAATGAAACATTTGATGAAGGTACTGCTATGGTTTCTGGTTCTCGATGTCTCGGTGTCTCCTTAAATGATACTCCTGTGATGAGTGGTTCTTTAGATGGTTCTGATGAACTTAAAAATTTAGATTCCACTGAGAGGAGTGTATCTTCTGACTCTCTTTTTGGGGCCGAATTACCTGGATCTACTTTGGACAAGGAATGCCCTATTGATACAACCCAAGAGACAGCAACAGGAGATAAGCCAGTGCTGGATTCTCCTGTTGAAGATAAAACTCAGTCTACCTCTCGCGGCCCTGGTGAGGCTCCGGCAGAAGAAGATGAGCCGGCGCTGGATTCTCCTGTTGAAGATAAAATACAGTCTACCTCTCAGGGTCCTGGTGAGGCTCCAGCAATAGAAGACGAACCAGTGCTGGATGATTCCCATGAAGAGAATGTTGATGCTCCCTGTCTTCAGCCTATTCCTTCTTCTGAAGAAACTTTCGCCGAGAACCCTGCTGAGGAAGTTAATGGAAATCAAGATTCCAATTTCTGTGGTGCTAATGAGAGTTCTTTGCCCAATAATTGTGATTTGAATATTCAACTTGATTGTGAGGCAAAAAACAAATCGTATGTGGCTTCAGAAGTCGCATCTGATGCAAGGTGGTGTGAAAAGAGCATTCCTCAAGCATCTAGTGATGGTTCCAAGGCTGATTCGGGGAACGCTGAATCTGGTTCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005700 GO:0006323 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006996 GO:0007063 GO:0007276 GO:0007283 GO:0008150 GO:0008270 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010639 GO:0010948 GO:0016043 GO:0016462 GO:0016581 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019219 GO:0019222 GO:0019953 GO:0022414 GO:0030261 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031491 GO:0031974 GO:0031981 GO:0032501 GO:0032504 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0034728 GO:0035327 GO:0042623 GO:0042766 GO:0043044 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044703 GO:0044877 GO:0045786 GO:0045875 GO:0045892 GO:0045934 GO:0046872 GO:0046914 GO:0048232 GO:0048519 GO:0048523 GO:0048609 GO:0050789 GO:0050794 GO:0051098 GO:0051101 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051312 GO:0051704 GO:0051726 GO:0051983 GO:0051985 GO:0060255 GO:0060341 GO:0065007 GO:0065009 GO:0070013 GO:0070603 GO:0070615 GO:0071103 GO:0071824 GO:0071840 GO:0071922 GO:0071923 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:1901363 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1903827 GO:1903828 GO:1904949 GO:1905634 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas PHD

Protein Analysis

1244

Amino Acids

137.3

Weight (kDa)

6.05

Isoelectric Point (pI)

54.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2484
AarI CACCTGC 2 cut(s) 740, 2974
Acc36I ACCTGC 3 cut(s) 740, 1226, 2974
AccB7I CCANNNNNTGG 2 cut(s) 503, 1745
AccBSI CCGCTC 1 cut(s) 2454
AccI GTMKAC 3 cut(s) 2055, 3290, 3371
AccII CGCG 1 cut(s) 3300
AciI CCGC 7 cut(s) 298, 1458, 1878, 2369, 2454, 2653, 3300
AcoI YGGCCR 1 cut(s) 1575
AcsI RAATTY 9 cut(s) 52, 284, 398, 518, 562, 1869, 1910, 2278, 3130
AcuI CTGAAG 6 cut(s) 294, 807, 1608, 3446, 3501, 3612
AdeI CACNNNGTG 1 cut(s) 503
AfaI GTAC 7 cut(s) 395, 569, 1223, 1240, 1347, 2590, 3037
AfiI CCNNNNNNNGG 8 cut(s) 34, 503, 1625, 1745, 2615, 3147, 3310, 3508
AflIII ACRYGT 1 cut(s) 541
AjnI CCWGG 7 cut(s) 1766, 2176, 2821, 2887, 3187, 3304, 3385
Alw21I GWGCWC 6 cut(s) 124, 643, 1054, 1682, 2271, 2690
Alw26I GTCTC 5 cut(s) 654, 1197, 3068, 3076, 3227
Alw44I GTGCAC 1 cut(s) 1678
AlwNI CAGNNNCTG 1 cut(s) 1787
Ama87I CYCGRG 1 cut(s) 86
AoxI GGCC 6 cut(s) 25, 755, 1187, 1575, 3177, 3301
ApaLI GTGCAC 1 cut(s) 1678
ApeKI GCWGC 3 cut(s) 697, 2151, 2567
ApoI RAATTY 9 cut(s) 52, 284, 398, 518, 562, 1869, 1910, 2278, 3130
ArsI GACNNNNNNTTYG 2 cut(s) 3155, 3187
Asp700I GAANNNNTTC 1 cut(s) 475
AspLEI GCGC 3 cut(s) 1276, 1941, 3340
AspS9I GGNCC 7 cut(s) 26, 755, 992, 1251, 3177, 3302, 3383
AsuHPI GGTGA 8 cut(s) 55, 467, 742, 1436, 2072, 2955, 3320, 3401
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 3 cut(s) 992, 1251, 3383
BaeGI GKGCMC 1 cut(s) 1682
BaeI ACNNNNGTAYC 2 cut(s) 3075, 3108
BalI TGGCCA 1 cut(s) 1577
BanII GRGCYC 3 cut(s) 643, 950, 2271
BarI GAAGNNNNNNTAC 4 cut(s) 551, 583, 3348, 3380
BauI CACGAG 1 cut(s) 2982
BbsI GAAGAC 4 cut(s) 544, 1614, 3414, 3452
Bbv12I GWGCWC 6 cut(s) 124, 643, 1054, 1682, 2271, 2690
BbvCI CCTCAGC 2 cut(s) 1419, 3507
BbvI GCAGC 3 cut(s) 684, 2138, 2554
BcgI CGANNNNNNTGC 6 cut(s) 405, 439, 475, 509, 3485, 3519
BciT130I CCWGG 7 cut(s) 1768, 2178, 2823, 2889, 3189, 3306, 3387
BcoDI GTCTC 5 cut(s) 654, 1197, 3068, 3076, 3227
BfmI CTRYAG 6 cut(s) 255, 698, 1885, 1985, 2515, 2837
BfoI RGCGCY 1 cut(s) 3341
BfuAI ACCTGC 3 cut(s) 740, 1226, 2974
BglII AGATCT 1 cut(s) 17
BisI GCNGC 6 cut(s) 698, 2152, 2455, 2568, 2653, 3301
BlpI GCTNAGC 1 cut(s) 642
BlsI GCNGC 6 cut(s) 699, 2153, 2456, 2569, 2654, 3302
Bme1390I CCNGG 7 cut(s) 1768, 2178, 2823, 2889, 3189, 3306, 3387
Bme18I GGWCC 3 cut(s) 992, 1251, 3383
BmeT110I CYCGRG 1 cut(s) 86
BmgT120I GGNCC 7 cut(s) 26, 755, 992, 1251, 3177, 3302, 3383
BmiI GGNNCC 7 cut(s) 219, 2366, 3178, 3315, 3384, 3396, 3691
BmrFI CCNGG 7 cut(s) 1768, 2178, 2823, 2889, 3189, 3306, 3387
BmrI ACTGGG 2 cut(s) 964, 1755
BmuI ACTGGG 2 cut(s) 964, 1755
BpiI GAAGAC 4 cut(s) 544, 1614, 3414, 3452
BplI GAGNNNNNCTC 2 cut(s) 3655, 3687
BpmI CTGGAG 4 cut(s) 782, 1865, 2160, 3381
Bpu10I CCTNAGC 2 cut(s) 1419, 3507
Bpu1102I GCTNAGC 1 cut(s) 642
BpuEI CTTGAG 3 cut(s) 190, 2678, 3657
Bsa29I ATCGAT 3 cut(s) 2111, 2379, 2495
BsaAI YACGTR 1 cut(s) 1136
BsaJI CCNNGG 6 cut(s) 87, 141, 2821, 2887, 3304, 3693
BsaWI WCCGGW 1 cut(s) 313
Bsc4I CCNNNNNNNGG 8 cut(s) 34, 503, 1625, 1745, 2615, 3147, 3310, 3508
Bse118I RCCGGY 2 cut(s) 2655, 3334
Bse1I ACTGG 8 cut(s) 765, 875, 959, 1253, 1750, 3000, 3254, 3416
Bse3DI GCAATG 3 cut(s) 267, 923, 2869
BseBI CCWGG 7 cut(s) 1768, 2178, 2823, 2889, 3189, 3306, 3387
BseCI ATCGAT 3 cut(s) 2111, 2379, 2495
BseDI CCNNGG 6 cut(s) 87, 141, 2821, 2887, 3304, 3693
BseLI CCNNNNNNNGG 8 cut(s) 34, 503, 1625, 1745, 2615, 3147, 3310, 3508
BseMI GCAATG 3 cut(s) 267, 923, 2869
BseNI ACTGG 8 cut(s) 765, 875, 959, 1253, 1750, 3000, 3254, 3416
BseRI GAGGAG 7 cut(s) 192, 1062, 1310, 1452, 1520, 2464, 3163
BseSI GKGCMC 1 cut(s) 1682
BseXI GCAGC 3 cut(s) 684, 2138, 2554
BseYI CCCAGC 1 cut(s) 1231
BsgI GTGCAG 2 cut(s) 1185, 1829
Bsh1236I CGCG 1 cut(s) 3300
BshFI GGCC 6 cut(s) 27, 757, 1189, 1577, 3179, 3303
BshVI ATCGAT 3 cut(s) 2111, 2379, 2495
BsiHKAI GWGCWC 6 cut(s) 124, 643, 1054, 1682, 2271, 2690
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 4 cut(s) 314, 2656, 3317, 3335
BslI CCNNNNNNNGG 8 cut(s) 34, 503, 1625, 1745, 2615, 3147, 3310, 3508
BsmAI GTCTC 5 cut(s) 654, 1197, 3068, 3076, 3227
BsmI GAATGC 5 cut(s) 751, 2429, 2912, 3215, 3666
BsnI GGCC 6 cut(s) 27, 757, 1189, 1577, 3179, 3303
BsoBI CYCGRG 1 cut(s) 86
Bsp1286I GDGCHC 7 cut(s) 124, 643, 950, 1054, 1682, 2271, 2690
Bsp1407I TGTACA 1 cut(s) 1221
Bsp1720I GCTNAGC 1 cut(s) 642
BspACI CCGC 7 cut(s) 298, 1458, 1878, 2369, 2454, 2653, 3300
BspANI GGCC 6 cut(s) 27, 757, 1189, 1577, 3179, 3303
BspDI ATCGAT 3 cut(s) 2111, 2379, 2495
BspFNI CGCG 1 cut(s) 3300
BspLI GGNNCC 7 cut(s) 219, 2366, 3178, 3315, 3384, 3396, 3691
BspMAI CTGCAG 2 cut(s) 702, 1989
BspMI ACCTGC 3 cut(s) 740, 1226, 2974
BsrBI CCGCTC 1 cut(s) 2454
BsrDI GCAATG 3 cut(s) 267, 923, 2869
BsrFI RCCGGY 2 cut(s) 2655, 3334
BsrGI TGTACA 1 cut(s) 1221
BsrI ACTGG 8 cut(s) 765, 875, 959, 1253, 1750, 3000, 3254, 3416
BssAI RCCGGY 2 cut(s) 2655, 3334
BssECI CCNNGG 6 cut(s) 87, 141, 2821, 2887, 3304, 3693
BssSI CACGAG 1 cut(s) 2982
BssT1I CCWWGG 2 cut(s) 141, 3693
Bst2BI CACGAG 1 cut(s) 2982
Bst2UI CCWGG 7 cut(s) 1768, 2178, 2823, 2889, 3189, 3306, 3387
Bst4CI ACNGT 9 cut(s) 438, 895, 941, 1620, 1789, 2060, 2413, 2519, 3369
Bst6I CTCTTC 4 cut(s) 146, 1019, 2245, 3432
BstAPI GCANNNNNTGC 1 cut(s) 3644
BstAUI TGTACA 1 cut(s) 1221
BstBAI YACGTR 1 cut(s) 1136
BstC8I GCNNGC 5 cut(s) 1168, 1943, 2397, 2657, 3336
BstFNI CGCG 1 cut(s) 3300
BstH2I RGCGCY 1 cut(s) 3341
BstHHI GCGC 3 cut(s) 1276, 1941, 3340
BstMAI GTCTC 5 cut(s) 654, 1197, 3068, 3076, 3227
BstMWI GCNNNNNNNGC 8 cut(s) 333, 827, 1241, 1271, 1282, 1768, 1938, 3644
BstNI CCWGG 7 cut(s) 1768, 2178, 2823, 2889, 3189, 3306, 3387
BstNSI RCATGY 2 cut(s) 545, 2836
BstSCI CCNGG 7 cut(s) 1766, 2176, 2821, 2887, 3187, 3304, 3385
BstSFI CTRYAG 6 cut(s) 255, 698, 1885, 1985, 2515, 2837
BstSLI GKGCMC 1 cut(s) 1682
BstUI CGCG 1 cut(s) 3300
BstV1I GCAGC 3 cut(s) 684, 2138, 2554
BstV2I GAAGAC 4 cut(s) 544, 1614, 3414, 3452
BstX2I RGATCY 4 cut(s) 17, 1454, 2987, 3191
BstYI RGATCY 4 cut(s) 17, 1454, 2987, 3191
Bsu15I ATCGAT 3 cut(s) 2111, 2379, 2495
BsuRI GGCC 6 cut(s) 27, 757, 1189, 1577, 3179, 3303
BsuTUI ATCGAT 3 cut(s) 2111, 2379, 2495
BtgZI GCGATG 1 cut(s) 2565
BtsI GCAGTG 3 cut(s) 1873, 1994, 2529
BveI ACCTGC 3 cut(s) 740, 1226, 2974
Cac8I GCNNGC 5 cut(s) 1168, 1943, 2397, 2657, 3336
CaiI CAGNNNCTG 1 cut(s) 1787
CfoI GCGC 3 cut(s) 1276, 1941, 3340
Cfr10I RCCGGY 2 cut(s) 2655, 3334
Cfr13I GGNCC 7 cut(s) 26, 755, 992, 1251, 3177, 3302, 3383
ClaI ATCGAT 3 cut(s) 2111, 2379, 2495
Csp6I GTAC 7 cut(s) 394, 568, 1222, 1239, 1346, 2589, 3036
CspCI CAANNNNNGTGG 4 cut(s) 984, 1019, 3523, 3558
CviQI GTAC 7 cut(s) 394, 568, 1222, 1239, 1346, 2589, 3036
DraIII CACNNNGTG 1 cut(s) 503
EaeI YGGCCR 1 cut(s) 1575
Eam1104I CTCTTC 4 cut(s) 146, 1019, 2245, 3432
EarI CTCTTC 4 cut(s) 146, 1019, 2245, 3432
Ecl136II GAGCTC 2 cut(s) 641, 2269
Eco130I CCWWGG 2 cut(s) 141, 3693
Eco147I AGGCCT 1 cut(s) 1189
Eco24I GRGCYC 3 cut(s) 643, 950, 2271
Eco47I GGWCC 3 cut(s) 992, 1251, 3383
Eco53kI GAGCTC 2 cut(s) 641, 2269
Eco57I CTGAAG 6 cut(s) 294, 807, 1608, 3446, 3501, 3612
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 2 cut(s) 641, 2269
EcoO109I RGGNCCY 1 cut(s) 3383
EcoRI GAATTC 2 cut(s) 398, 1869
EcoRII CCWGG 7 cut(s) 1766, 2176, 2821, 2887, 3187, 3304, 3385
EcoT14I CCWWGG 2 cut(s) 141, 3693
EcoT22I ATGCAT 2 cut(s) 2005, 2739
EcoT38I GRGCYC 3 cut(s) 643, 950, 2271
ErhI CCWWGG 2 cut(s) 141, 3693
FalI AAGNNNNNCTT 2 cut(s) 1666, 1698
FauI CCCGC 2 cut(s) 291, 2376
FauNDI CATATG 1 cut(s) 1641
FblI GTMKAC 3 cut(s) 2055, 3290, 3371
Fnu4HI GCNGC 6 cut(s) 698, 2152, 2455, 2568, 2653, 3301
FriOI GRGCYC 3 cut(s) 643, 950, 2271
Fsp4HI GCNGC 6 cut(s) 698, 2152, 2455, 2568, 2653, 3301
GlaI GCGC 3 cut(s) 1275, 1940, 3339
GluI GCNGC 6 cut(s) 698, 2152, 2455, 2568, 2653, 3301
GsaI CCCAGC 1 cut(s) 1235
GsuI CTGGAG 4 cut(s) 782, 1865, 2160, 3381
HaeII RGCGCY 1 cut(s) 3341
HaeIII GGCC 6 cut(s) 27, 757, 1189, 1577, 3179, 3303
HapII CCGG 4 cut(s) 314, 2656, 3317, 3335
HhaI GCGC 3 cut(s) 1276, 1941, 3340
Hin6I GCGC 3 cut(s) 1274, 1939, 3338
HinP1I GCGC 3 cut(s) 1274, 1939, 3338
HincII GTYRAC 4 cut(s) 550, 1714, 2056, 2443
HindII GTYRAC 4 cut(s) 550, 1714, 2056, 2443
HindIII AAGCTT 3 cut(s) 860, 1667, 2393
HpaII CCGG 4 cut(s) 314, 2656, 3317, 3335
HphI GGTGA 8 cut(s) 55, 467, 742, 1436, 2072, 2955, 3320, 3401
Hpy99I CGWCG 1 cut(s) 2060
HpyCH4III ACNGT 9 cut(s) 438, 895, 941, 1620, 1789, 2060, 2413, 2519, 3369
HpyCH4IV ACGT 4 cut(s) 1105, 1135, 1757, 2874
HpyF10VI GCNNNNNNNGC 8 cut(s) 333, 827, 1241, 1271, 1282, 1768, 1938, 3644
HpySE526I ACGT 4 cut(s) 1105, 1135, 1757, 2874
HspAI GCGC 3 cut(s) 1274, 1939, 3338
KroI GCCGGC 2 cut(s) 2655, 3334
KroNI GCCGGC 2 cut(s) 2657, 3336
LmnI GCTCC 7 cut(s) 482, 638, 1057, 2451, 3319, 3400, 3457
Lsp1109I GCAGC 3 cut(s) 684, 2138, 2554
MaeII ACGT 4 cut(s) 1105, 1135, 1757, 2874
MaeIII GTNAC 5 cut(s) 809, 2020, 2060, 2180, 2875
MbiI CCGCTC 1 cut(s) 2454
MfeI CAATTG 3 cut(s) 1286, 1793, 2721
MflI RGATCY 4 cut(s) 17, 1454, 2987, 3191
MhlI GDGCHC 7 cut(s) 124, 643, 950, 1054, 1682, 2271, 2690
MlsI TGGCCA 1 cut(s) 1577
MluNI TGGCCA 1 cut(s) 1577
MlyI GAGTC 6 cut(s) 1063, 1449, 1772, 1961, 2231, 3158
MmeI TCCRAC 1 cut(s) 366
Mox20I TGGCCA 1 cut(s) 1577
Mph1103I ATGCAT 2 cut(s) 2005, 2739
MroNI GCCGGC 2 cut(s) 2655, 3334
MroXI GAANNNNTTC 1 cut(s) 475
MscI TGGCCA 1 cut(s) 1577
MslI CAYNNNNRTG 3 cut(s) 909, 2937, 3015
Msp20I TGGCCA 1 cut(s) 1577
MspI CCGG 4 cut(s) 314, 2656, 3317, 3335
MspR9I CCNGG 7 cut(s) 1768, 2178, 2823, 2889, 3189, 3306, 3387
MunI CAATTG 3 cut(s) 1286, 1793, 2721
Mva1269I GAATGC 5 cut(s) 751, 2429, 2912, 3215, 3666
MvaI CCWGG 7 cut(s) 1768, 2178, 2823, 2889, 3189, 3306, 3387
MvnI CGCG 1 cut(s) 3300
MwoI GCNNNNNNNGC 8 cut(s) 333, 827, 1241, 1271, 1282, 1768, 1938, 3644
NaeI GCCGGC 2 cut(s) 2657, 3336
NdeI CATATG 1 cut(s) 1641
NgoMIV GCCGGC 2 cut(s) 2655, 3334
NlaIV GGNNCC 7 cut(s) 219, 2366, 3178, 3315, 3384, 3396, 3691
NmeAIII GCCGAG 1 cut(s) 3520
NmuCI GTSAC 4 cut(s) 809, 2060, 2180, 2875
NsiI ATGCAT 2 cut(s) 2005, 2739
NspI RCATGY 2 cut(s) 545, 2836
PaqCI CACCTGC 2 cut(s) 740, 2974
PceI AGGCCT 1 cut(s) 1189
PciI ACATGT 1 cut(s) 541
PcsI WCGNNNNNNNCGW 1 cut(s) 2061
PctI GAATGC 5 cut(s) 751, 2429, 2912, 3215, 3666
PdiI GCCGGC 2 cut(s) 2657, 3336
PdmI GAANNNNTTC 1 cut(s) 475
PflMI CCANNNNNTGG 2 cut(s) 503, 1745
PkrI GCNGC 6 cut(s) 699, 2153, 2456, 2569, 2654, 3302
PleI GAGTC 6 cut(s) 1063, 1448, 1772, 1961, 2231, 3158
PpsI GAGTC 6 cut(s) 1063, 1448, 1772, 1961, 2231, 3158
Ppu21I YACGTR 1 cut(s) 1136
PpuMI RGGWCCY 1 cut(s) 3383
PscI ACATGT 1 cut(s) 541
PsiI TTATAA 1 cut(s) 2484
Psp124BI GAGCTC 2 cut(s) 643, 2271
Psp5II RGGWCCY 1 cut(s) 3383
Psp6I CCWGG 7 cut(s) 1766, 2176, 2821, 2887, 3187, 3304, 3385
PspFI CCCAGC 1 cut(s) 1231
PspGI CCWGG 7 cut(s) 1766, 2176, 2821, 2887, 3187, 3304, 3385
PspN4I GGNNCC 7 cut(s) 219, 2366, 3178, 3315, 3384, 3396, 3691
PspPI GGNCC 7 cut(s) 26, 755, 992, 1251, 3177, 3302, 3383
PspPPI RGGWCCY 1 cut(s) 3383
PstI CTGCAG 2 cut(s) 702, 1989
PstNI CAGNNNCTG 1 cut(s) 1787
PsuI RGATCY 4 cut(s) 17, 1454, 2987, 3191
RsaI GTAC 7 cut(s) 395, 569, 1223, 1240, 1347, 2590, 3037
RsaNI GTAC 7 cut(s) 394, 568, 1222, 1239, 1346, 2589, 3036
RseI CAYNNNNRTG 3 cut(s) 909, 2937, 3015
SacI GAGCTC 2 cut(s) 643, 2271
SalI GTCGAC 1 cut(s) 2054
SatI GCNGC 6 cut(s) 698, 2152, 2455, 2568, 2653, 3301
Sau96I GGNCC 7 cut(s) 26, 755, 992, 1251, 3177, 3302, 3383
SchI GAGTC 6 cut(s) 1063, 1449, 1772, 1961, 2231, 3158
ScrFI CCNGG 7 cut(s) 1768, 2178, 2823, 2889, 3189, 3306, 3387
SduI GDGCHC 7 cut(s) 124, 643, 950, 1054, 1682, 2271, 2690
SfcI CTRYAG 6 cut(s) 255, 698, 1885, 1985, 2515, 2837
SinI GGWCC 3 cut(s) 992, 1251, 3383
SmiMI CAYNNNNRTG 3 cut(s) 909, 2937, 3015
SmlI CTYRAG 3 cut(s) 169, 2693, 3672
SmoI CTYRAG 3 cut(s) 169, 2693, 3672
SseBI AGGCCT 1 cut(s) 1189
SsiI CCGC 7 cut(s) 298, 1458, 1878, 2369, 2454, 2653, 3300
SspI AATATT 2 cut(s) 817, 3586
SstI GAGCTC 2 cut(s) 643, 2271
StuI AGGCCT 1 cut(s) 1189
StyD4I CCNGG 7 cut(s) 1766, 2176, 2821, 2887, 3187, 3304, 3385
StyI CCWWGG 2 cut(s) 141, 3693
TaaI ACNGT 9 cut(s) 438, 895, 941, 1620, 1789, 2060, 2413, 2519, 3369
TaiI ACGT 4 cut(s) 1108, 1138, 1760, 2877
TatI WGTACW 3 cut(s) 567, 1221, 1345
TauI GCSGC 3 cut(s) 2457, 2655, 3303
TseFI GTSAC 4 cut(s) 809, 2060, 2180, 2875
TseI GCWGC 3 cut(s) 697, 2151, 2567
Tsp45I GTSAC 4 cut(s) 809, 2060, 2180, 2875
TspGWI ACGGA 5 cut(s) 1133, 1433, 1768, 2035, 2179
Van91I CCANNNNNTGG 2 cut(s) 503, 1745
VneI GTGCAC 1 cut(s) 1678
VpaK11BI GGWCC 3 cut(s) 992, 1251, 3383
XapI RAATTY 9 cut(s) 52, 284, 398, 518, 562, 1869, 1910, 2278, 3130
XbaI TCTAGA 1 cut(s) 1210
XceI RCATGY 2 cut(s) 545, 2836
XmiI GTMKAC 3 cut(s) 2055, 3290, 3371
XmnI GAANNNNTTC 1 cut(s) 475
Zsp2I ATGCAT 2 cut(s) 2005, 2739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.