Rh4DG199700

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007814
Physical Location & Seq
Reverse (-)
38853972 .. 38864099
10128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG199700.1

Sequence Viewer

Length: 4665 bp
ATGGACGAAGGTGTGAGATCTGTTGGCCCTTCTGGGGTGTTGGTGAAGAATAGGAATTCTTCGGGGTGTTTAATTGTGAGGAAGAAGCCCGATGGGGTTAGTGGTGGCGTTGGTTCTTCGAGTTCGAGGAAGGTTTTTGAATCGAGTTCGAGGAAGGAGAAGAAGAGGTCGAGGTTGGTGATGAGTGATTCAGGATCGAGTGATGAGCTGTTGATGCCGAGGAGGAAAGTTGGGCCGGAGACTATTCGGGTTTGTAATGGTTTGGACAAGGGCATTGTGGAAGGGAGCGGAATGGTTCCGAAGAGGGAGAGACTGGAGCATGGTAGGCGGAATGAGGATGGTATGATTGGTACAAATTATTTGGATGATGGGGGAGGAAGGAGAACTAATTTGGAGGTGTTTGAGTTCAATGAGTATGATGGGATTGAGGGACAACCGATGCGGAGGAGTCGGTTCGGCGATGGAGTTATCGGAGTTGATTTTGGGGAGAGAAGATATGCCGGTGTACCCCGAGGTGGCATTAAGAGGGAATTTGAGACTGGTTCAAGTAGACATCTTGTTGACAAGAGAAAGAACCTGTATCATGACCGGACAAGTAGCTTGAACCGGGGAGATCGTGCTGATAGGGGTAGGTACGAGATGAGTGGCGATGGAGCTCAGCTGCCCTTATTGAGAGACAAGTACGTGGGTGTTTCAGATGAACCCATTAGGGTCCAGGGTAAAAATGGTGTTTTGAAGGTGATGGTGAAGAAGAAAAACAATGTGCCTGGGCCACTAGGGAACTATAGTTTTCCAAAATCTGAAGAACATAGAAAAGCTCCAAGAAGTGAGGATATTTCCAAGAAGAATGCTATCATCCCTCCACTTTTCGCAGAACCAAAGCTTCCTGAGAAACCTGTTTCAGCTGTTAGGACAGAGAAAAACCATGTGAATTTGCGAAAATCATTGCCCGCTAAGAACAGCAAGAGCTCTGACTGGGATTCGGAGGACAGTGATACCTCATTGAAGATAGCAGCAAAGAGTGCCGAAGCCTCTAAACCTTTGAAGAGGGCAGGTTGCAAAGTTGAAGATGGTCCTTCATCTGAAAAGACCCCTCCAGCCAGAAATAAAGAAGTCAAACTTAAACGTGGTAGTGGCACAGAGAAGCAGAAACTGCGTGAACGGATAAGGGAGATGCTCCTGGATGCAGGCTGGACCATAGATTATAGGCCTAGAAGGAACAGAGACTACCTGGATGCTGTGTACATTAACCCATCAGGTACAGCTTACTGGTCTATCATTAAGGCTTATGACGCACTTCAAAAGCAAATAAATGAGGAAAATGAGGCTAAACGTAATGGGGATGGTTCTTCACTTGCACCTATAACTGATGACGTACTCAGTCAGTTGACCAGGAAAACTCGAAAGAAGATGGAGAAAGAAATGAAAAGGAAGCAAAGAGCTGACAGTGACAGTGAGAATGCAAAAGGAGTCCGTGTGAAAAAACCTAAAAGTGCCAAGCATGATCCAGAGAGCATGGACAGTGTTAGTTATGAGGAGAAATTAAGCTCCTACTTGAAGCAAGGTGGTAAGTCATTCAAGGGTAGAATGTATGAAAACGGTTTGGATTCAAATGGTCAGAGTTCCTCTCATCATTTACATGGCACTGTTGAAAAACCATCTTCTGGATCTAGTTCTCACATGCCACATGGAAGGAAAAGTAGAAAGCTTGGTAGATGTACTTTGTTGGTTCGTAGTTCTAAAAAGGGGCCTAACTCGGAAAGTGATGGCTTTGTTCCATATACTGGAAAACGAACATTGCTCTCCTGGCTGATTGATACTGGAACTGTGCAATTGAGCCAAAAGGTGCAGTATATGAATCGGCGAAGAACTAAAGTAATGCTGGAGGGCTGGATTACAAGAGATGGCATTCACTGTGGTTGCTGTAGTAAGATCCTCACAGTCTCGAAATTTGAGATTCATGCAGGGAGCAAATTGCGCCAGCCATTTCAAAATATATGCTTGGACTCTGGGGTTTCCCTTCTGCAATGTCAGATAGATGCGTGGAATAGACAAGAGGATATTGAACGCATTGGTTTCCACTCTGTACAAGTTGACGGTGACGATCCCGATGATGATACTTGTGGCCTCTGTGGAGATGGTGGAGATTTGATCTGTTGTGATGGCTGTCCTTCGACATTTCATCAGAGCTGCTTGAACATACAGAAGCTTCCTCCAGGTGATTGGCACTGCCCAAATTGTATTTGCAAAGTTTGTGGAATAGCTAGTGAGGTTGTTGCTGAAGAGGATGATGCAACTGTTTCTGCACTACTGGCTTGTAGTCTCTGTGGGAAGAAATGTCACATATCATGCAGTCAGGAGATGGATGCTAGTCCTGCTGATTCCAATAGTTCAGGCTCTTCCTTTTGTGGGCAGAGATGTAGAGAGCTCTTTGAGAATTTGCAGAGATGTCTTGGGGTCAAACATGAACTAGAAGCAGGATATACGTGGTCACTTGTAAAGAGAACAGATGTTGATAGAGGATTTCCTCTGAGGGTTGAATGCAATTCTAAGCTCGCTGTTGCATTGACCGTTATGGATGAATGCTTTTTGCCTATTGTTGACAGACGGAGTGGGATCAATTTAATCCATAATGTTCTATATAATTGTGGATCAAACTTCAATCGGCTGAACTACAGTGGCTTCTATGCTGCTATTTTGGAGAAAGGTGATGAAATAATATCTGCCGCTTCTATCAGGTTTCATGGTACAATGTTGGCAGAGATGCCATTCATCGGAACTCGTCACATATACAGGCGACAAGGGATGTGTCGTAGGCTTTTCTGCGCCATTGAATCGGCTCTCTGCTCCCTGAAGGTTGAGAAGTTGGTTATCCCTGCAATTGCGGAACTCATGCATACATGGACAGGTGTTTTTGGTTTTGTTCCTCTTGAGGAATCATTCAAGCAAGAAGTGAGGTCAATCAATATGTTAGTCTTCCCTGGTATAGACATGCTACAGAAGCTGCTGGTGGACAAAGAAAATGAGACAAACATGACAGGTGTAAAGAAAATGGACGGCATAGGCAAGGGTTGTATAAAGCCTGGAGGAGGAGGCAAATCTGATATTGGTTCCCCAGCTAGTCTTGATCATCATGGGAGTGATGGAGCTGGTATGCTTCATATCAATGAAACTGTAGATGAAGCTGCTGATGTGGACTCTGGTTCTCGATGTCTAGGTGTCTCCTTAAATGATACTCCTGTAATGAGTGGCTCTTTGGATGCCTCTGATGAACTCAAAAATTTAGATTCAACTGGAAGGAGTATGTCTTCCGAGTCTCTTGCCGGTGACCAATTAGCTGGATCTTCTTCTGATGGGAAATGCCTTCCTAATACAAGCCATGAAGTTCTCGAGATAGGAACTAAACCACTGCTGGAGTCTCCTGTTGAGGATAATATGCAGTGTAATTCCAAGTGTCCTGTTGTTTCTTCAAATAATATTTCCATGTTGAATGGTGGTTCATTAGATGCTTCTCATGAACAAAACAATCAGATTTCAATCAATGGGACTCTGTCTGCTGACTCTGATTCTGGTGCTAAGTTAGCTGAATATGGTTCTGAAGCAAAATTCCCATCTCCCCCTGAAAGAAGCAGTGAAGCTGTAGACACTGCACCTGTTGAAGATAATGTCTTGTCTACCTTGCAGTGTCCTGCTGCCTCATTAAATAACACTTCCATGATAAGGAGTAATCTAATGGAAGCTTCTCAGGTTCCCGCTAAGGGGACTGCATCTTCCGACTCCAATTCAGTTGCCAAGTTAGATGAATACACTTCTGAAGCAAAATGCCAGTCTCCCTCTAAAACAATTGATGAAGCTCTAGAGATTGGAAATGAAACAATACTGCATTCTCCTGTTGAAGGTAATATGCAGTCCACCTCTCAAGGTCCTGGTGCTCCATTAAATAATACTTCCATGTTGACTAGTAGCTCATCAGATGCTGCTTCTCATGAACTTAAGGTTTTGGATTCAACCAAGGGGAATCCATCTTTTGACTGTGATTCAGGTGCCGAGTTAACTGAACACACTTCTGAAGTGAAACACAAGTCTTCCCCTGTTACAAGACATGAAGTTCTAGAGACGGAAAATCAACCAGTGGCAGATTCTCCCATTGAAGATAATATCCAGTCTACCTCTCAGAGTCCTGATGCTTCGTTAAAGGATACTTCCATGTTGAGTAGTGGTTCACTGAATGCTTTCCATGAACTCAACATTCAGCCTTCAACCAAGGGGACTCCATCCAACTCTGATCAAGGCACCAAGTTAGCTGAGTACATGTTGGATGCAAAATGTCAGTCTTCTCCGGATACAAGTGATGATCAATTGATGGGAGTAAAACCATTCCTGGATAATTCTTCAAGGTGCTTGAAAGAAAGTGCTTCTGATAATTCCCATGAAGAGAATGTTGATAGGGCCTCCCTTGAGCCTGTTCCTTTTTCTGGAGAAACTTTTGCTGGGAGCAGTGAGGAAGATGGAAATCCAAATTCGAGTTTCTGTGAGACTGAGAAACAATCATACGTGGCTCCAGAAGTTGCTTCTGATGCAGTGCATTGTGAAAAGAGCATCCCTAAAGCATCTAGTGATGGCTCCGCTACTGATTCTGGGAGGGATGAATCTGGTTCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005700 GO:0006323 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006996 GO:0007063 GO:0007276 GO:0007283 GO:0008150 GO:0008270 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010639 GO:0010948 GO:0016043 GO:0016462 GO:0016581 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019219 GO:0019222 GO:0019953 GO:0022414 GO:0030261 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031491 GO:0031974 GO:0031981 GO:0032501 GO:0032504 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0034728 GO:0035327 GO:0042623 GO:0042766 GO:0043044 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044703 GO:0044877 GO:0045786 GO:0045875 GO:0045892 GO:0045934 GO:0046872 GO:0046914 GO:0048232 GO:0048519 GO:0048523 GO:0048609 GO:0050789 GO:0050794 GO:0051098 GO:0051101 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051312 GO:0051704 GO:0051726 GO:0051983 GO:0051985 GO:0060255 GO:0060341 GO:0065007 GO:0065009 GO:0070013 GO:0070603 GO:0070615 GO:0071103 GO:0071824 GO:0071840 GO:0071922 GO:0071923 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:1901363 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1903827 GO:1903828 GO:1904949 GO:1905634 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas PHD

Protein Analysis

1554

Amino Acids

168.83

Weight (kDa)

6.04

Isoelectric Point (pI)

52.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7028 PF22970 373 - 436 9.4e-15 Domain of unknown function (DUF7028)
TDBD PF16135 630 - 680 2.6e-21 Tify domain binding domain
PHD PF00628 708 - 747 4.6e-07 PHD-finger
IDM1_C PF23209 863 - 1001 2.5e-58 Increased DNA methylation 1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1043
AccB1I GGYRCC 2 cut(s) 4043, 4292
AccB7I CCANNNNNTGG 2 cut(s) 1664, 1784
AccBSI CCGCTC 1 cut(s) 288
AccI GTMKAC 4 cut(s) 550, 3642, 3674, 4166
AccIII TCCGGA 1 cut(s) 4339
AciI CCGC 8 cut(s) 288, 328, 442, 951, 2727, 2885, 3753, 4626
AclWI GGATC 8 cut(s) 202, 1499, 1675, 1927, 2099, 2624, 2659, 3349
AcsI RAATTY 8 cut(s) 55, 530, 931, 1949, 2437, 3280, 3605, 4519
AcuI CTGAAG 6 cut(s) 822, 2301, 2873, 3618, 3834, 4089
AflII CTTAAG 1 cut(s) 3992
AflIII ACRYGT 1 cut(s) 4311
AhlI ACTAGT 1 cut(s) 3959
Alw21I GWGCWC 4 cut(s) 658, 971, 2430, 3934
AlwI GGATC 8 cut(s) 202, 1499, 1675, 1927, 2099, 2624, 2659, 3349
AlwNI CAGNNNCTG 3 cut(s) 1153, 3004, 3977
Ama87I CYCGRG 2 cut(s) 510, 3389
Aor13HI TCCGGA 1 cut(s) 4339
AoxI GGCC 7 cut(s) 25, 233, 770, 1208, 1748, 2125, 4449
ApeKI GCWGC 8 cut(s) 661, 1013, 2190, 2690, 3004, 3185, 3692, 3977
ApoI RAATTY 8 cut(s) 55, 530, 931, 1949, 2437, 3280, 3605, 4519
ArsI GACNNNNNNTTYG 4 cut(s) 3812, 3844, 4316, 4348
Asp700I GAANNNNTTC 1 cut(s) 4232
AspLEI GCGC 2 cut(s) 1980, 2828
AspS9I GGNCC 9 cut(s) 26, 233, 712, 770, 1073, 1194, 1748, 3923, 4449
AsuC2I CCSGG 1 cut(s) 608
AsuHPI GGTGA 8 cut(s) 55, 190, 751, 757, 2111, 2231, 2720, 3338
AvaI CYCGRG 2 cut(s) 510, 3389
AvaII GGWCC 4 cut(s) 712, 1073, 1194, 3923
BaeI ACNNNNGTAYC 2 cut(s) 3225, 3258
BanI GGYRCC 2 cut(s) 4043, 4292
BanII GRGCYC 3 cut(s) 658, 971, 2430
BbsI GAAGAC 4 cut(s) 2968, 3300, 4077, 4326
Bbv12I GWGCWC 4 cut(s) 658, 971, 2430, 3934
BbvI GCAGC 8 cut(s) 648, 1025, 2177, 2677, 2991, 3172, 3679, 3964
BceAI ACGGC 1 cut(s) 3073
BcgI CGANNNNNNTGC 2 cut(s) 3302, 3336
BciVI GTATCC 2 cut(s) 4192, 4336
BclI TGATCA 3 cut(s) 3127, 4285, 4354
BcnI CCSGG 1 cut(s) 608
BcuI ACTAGT 1 cut(s) 3959
BfmI CTRYAG 6 cut(s) 784, 1924, 2674, 2996, 3174, 3639
BfrI CTTAAG 1 cut(s) 3992
BfuAI ACCTGC 1 cut(s) 1043
BfuI GTATCC 2 cut(s) 4192, 4336
BglII AGATCT 1 cut(s) 17
BisI GCNGC 9 cut(s) 662, 1014, 2191, 2691, 2727, 3005, 3186, 3693, 3978
BlpI GCTNAGC 1 cut(s) 657
BlsI GCNGC 9 cut(s) 663, 1015, 2192, 2692, 2728, 3006, 3187, 3694, 3979
Bme18I GGWCC 4 cut(s) 712, 1073, 1194, 3923
BmeT110I CYCGRG 2 cut(s) 510, 3389
BmgT120I GGNCC 9 cut(s) 26, 233, 712, 770, 1073, 1194, 1748, 3923, 4449
BmiI GGNNCC 9 cut(s) 297, 713, 1749, 3112, 3750, 4045, 4294, 4560, 4624
BmrI ACTGGG 1 cut(s) 985
BmuI ACTGGG 1 cut(s) 985
BpiI GAAGAC 4 cut(s) 2968, 3300, 4077, 4326
BplI GAGNNNNNCTC 2 cut(s) 1612, 1644
BpmI CTGGAG 8 cut(s) 335, 1080, 1904, 2199, 3105, 3434, 4497, 4545
Bpu10I CCTNAGC 1 cut(s) 3756
Bpu1102I GCTNAGC 1 cut(s) 657
BpuEI CTTGAG 3 cut(s) 2951, 3903, 4478
BpuMI CCSGG 1 cut(s) 608
BsaAI YACGTR 3 cut(s) 685, 2487, 4555
BsaBI GATNNNNATC 2 cut(s) 3536, 4512
BsaJI CCNNGG 8 cut(s) 218, 511, 607, 715, 767, 2980, 4011, 4263
BsaWI WCCGGW 2 cut(s) 588, 4339
BsaXI ACNNNNNCTCC 2 cut(s) 1461, 1491
Bse118I RCCGGY 2 cut(s) 500, 3323
Bse3DI GCAATG 3 cut(s) 944, 1796, 2033
Bse8I GATNNNNATC 2 cut(s) 3536, 4512
BseAI TCCGGA 1 cut(s) 4339
BseDI CCNNGG 8 cut(s) 218, 511, 607, 715, 767, 2980, 4011, 4263
BseJI GATNNNNATC 2 cut(s) 3536, 4512
BseMI GCAATG 3 cut(s) 944, 1796, 2033
BseMII CTCAG 8 cut(s) 671, 879, 1393, 2522, 3758, 4187, 4296, 4530
BseRI GAGGAG 5 cut(s) 235, 460, 1550, 3102, 3105
BseXI GCAGC 8 cut(s) 648, 1025, 2177, 2677, 2991, 3172, 3679, 3964
BseYI CCCAGC 2 cut(s) 3115, 4490
BsgI GTGCAG 3 cut(s) 1868, 2289, 3633
BshFI GGCC 7 cut(s) 27, 235, 772, 1210, 1750, 2127, 4451
BshNI GGYRCC 2 cut(s) 4043, 4292
BsiHKAI GWGCWC 4 cut(s) 658, 971, 2430, 3934
BsiHKCI CYCGRG 2 cut(s) 510, 3389
BsiSI CCGG 6 cut(s) 236, 501, 589, 607, 3324, 4340
BslFI GGGAC 4 cut(s) 444, 3559, 3775, 4282
BsmBI CGTCTC 1 cut(s) 4109
BsmFI GGGAC 4 cut(s) 444, 3559, 3775, 4282
BsmI GAATGC 7 cut(s) 853, 1465, 1908, 2546, 2588, 3883, 4234
BsnI GGCC 7 cut(s) 27, 235, 772, 1210, 1750, 2127, 4451
BsoBI CYCGRG 2 cut(s) 510, 3389
Bsp1286I GDGCHC 4 cut(s) 658, 971, 2430, 3934
Bsp13I TCCGGA 1 cut(s) 4339
Bsp1407I TGTACA 2 cut(s) 1242, 2086
Bsp1720I GCTNAGC 1 cut(s) 657
BspACI CCGC 8 cut(s) 288, 328, 442, 951, 2727, 2885, 3753, 4626
BspANI GGCC 7 cut(s) 27, 235, 772, 1210, 1750, 2127, 4451
BspCNI CTCAG 8 cut(s) 670, 880, 1392, 2523, 3757, 4186, 4297, 4531
BspEI TCCGGA 1 cut(s) 4339
BspHI TCATGA 3 cut(s) 583, 3514, 3985
BspLI GGNNCC 9 cut(s) 297, 713, 1749, 3112, 3750, 4045, 4294, 4560, 4624
BspMI ACCTGC 1 cut(s) 1043
BspPI GGATC 8 cut(s) 202, 1499, 1675, 1927, 2099, 2624, 2659, 3349
BspQI GCTCTTC 1 cut(s) 2404
BspT107I GGYRCC 2 cut(s) 4043, 4292
BspTI CTTAAG 1 cut(s) 3992
BsrBI CCGCTC 1 cut(s) 288
BsrDI GCAATG 3 cut(s) 944, 1796, 2033
BsrFI RCCGGY 2 cut(s) 500, 3323
BsrGI TGTACA 2 cut(s) 1242, 2086
BssAI RCCGGY 2 cut(s) 500, 3323
BssECI CCNNGG 8 cut(s) 218, 511, 607, 715, 767, 2980, 4011, 4263
BssT1I CCWWGG 2 cut(s) 4011, 4263
Bst6I CTCTTC 6 cut(s) 158, 296, 1040, 2277, 2404, 4428
BstAFI CTTAAG 1 cut(s) 3992
BstAPI GCANNNNNTGC 2 cut(s) 1022, 1153
BstAUI TGTACA 2 cut(s) 1242, 2086
BstBAI YACGTR 3 cut(s) 685, 2487, 4555
BstC8I GCNNGC 4 cut(s) 951, 1189, 1982, 2556
BstEII GGTNACC 1 cut(s) 3326
BstENI CCTNNNNNAGG 2 cut(s) 3087, 3894
BstHHI GCGC 2 cut(s) 1980, 2828
BstNSI RCATGY 3 cut(s) 1684, 2995, 4315
BstPI GGTNACC 1 cut(s) 3326
BstSFI CTRYAG 6 cut(s) 784, 1924, 2674, 2996, 3174, 3639
BstV1I GCAGC 8 cut(s) 648, 1025, 2177, 2677, 2991, 3172, 3679, 3964
BstV2I GAAGAC 4 cut(s) 2968, 3300, 4077, 4326
BstX2I RGATCY 4 cut(s) 17, 1667, 1932, 3341
BstXI CCANNNNNNTGG 2 cut(s) 2223, 3338
BstYI RGATCY 4 cut(s) 17, 1667, 1932, 3341
BsuI GTATCC 2 cut(s) 4192, 4336
BsuRI GGCC 7 cut(s) 27, 235, 772, 1210, 1750, 2127, 4451
BtgZI GCGATG 2 cut(s) 474, 663
BtsI GCAGTG 8 cut(s) 2227, 3407, 3446, 3637, 3645, 3689, 4504, 4587
BveI ACCTGC 1 cut(s) 1043
Cac8I GCNNGC 4 cut(s) 951, 1189, 1982, 2556
CaiI CAGNNNCTG 3 cut(s) 1153, 3004, 3977
CciI TCATGA 3 cut(s) 583, 3514, 3985
CfoI GCGC 2 cut(s) 1980, 2828
Cfr10I RCCGGY 2 cut(s) 500, 3323
Cfr13I GGNCC 9 cut(s) 26, 233, 712, 770, 1073, 1194, 1748, 3923, 4449
CseI GACGC 1 cut(s) 1301
CspCI CAANNNNNGTGG 2 cut(s) 2236, 2271
Eam1104I CTCTTC 6 cut(s) 158, 296, 1040, 2277, 2404, 4428
EarI CTCTTC 6 cut(s) 158, 296, 1040, 2277, 2404, 4428
EciI GGCGGA 1 cut(s) 343
Ecl136II GAGCTC 3 cut(s) 656, 969, 2428
Eco130I CCWWGG 2 cut(s) 4011, 4263
Eco147I AGGCCT 1 cut(s) 1210
Eco24I GRGCYC 3 cut(s) 658, 971, 2430
Eco47I GGWCC 4 cut(s) 712, 1073, 1194, 3923
Eco53kI GAGCTC 3 cut(s) 656, 969, 2428
Eco57I CTGAAG 6 cut(s) 822, 2301, 2873, 3618, 3834, 4089
Eco88I CYCGRG 2 cut(s) 510, 3389
Eco91I GGTNACC 1 cut(s) 3326
EcoICRI GAGCTC 3 cut(s) 656, 969, 2428
EcoNI CCTNNNNNAGG 2 cut(s) 3087, 3894
EcoO109I RGGNCCY 3 cut(s) 1748, 3923, 4449
EcoO65I GGTNACC 1 cut(s) 3326
EcoRI GAATTC 1 cut(s) 55
EcoT14I CCWWGG 2 cut(s) 4011, 4263
EcoT22I ATGCAT 1 cut(s) 2898
EcoT38I GRGCYC 3 cut(s) 658, 971, 2430
ErhI CCWWGG 2 cut(s) 4011, 4263
Esp3I CGTCTC 1 cut(s) 4109
FalI AAGNNNNNCTT 2 cut(s) 1104, 1136
FaqI GGGAC 4 cut(s) 444, 3559, 3775, 4282
FauI CCCGC 2 cut(s) 958, 3760
FbaI TGATCA 3 cut(s) 3127, 4285, 4354
FblI GTMKAC 4 cut(s) 550, 3642, 3674, 4166
Fnu4HI GCNGC 9 cut(s) 662, 1014, 2191, 2691, 2727, 3005, 3186, 3693, 3978
FriOI GRGCYC 3 cut(s) 658, 971, 2430
Fsp4HI GCNGC 9 cut(s) 662, 1014, 2191, 2691, 2727, 3005, 3186, 3693, 3978
GlaI GCGC 2 cut(s) 1979, 2827
GluI GCNGC 9 cut(s) 662, 1014, 2191, 2691, 2727, 3005, 3186, 3693, 3978
GsaI CCCAGC 2 cut(s) 3119, 4494
GsuI CTGGAG 8 cut(s) 335, 1080, 1904, 2199, 3105, 3434, 4497, 4545
HaeIII GGCC 7 cut(s) 27, 235, 772, 1210, 1750, 2127, 4451
HapII CCGG 6 cut(s) 236, 501, 589, 607, 3324, 4340
HgaI GACGC 1 cut(s) 1301
HhaI GCGC 2 cut(s) 1980, 2828
Hin6I GCGC 2 cut(s) 1978, 2826
HinP1I GCGC 2 cut(s) 1978, 2826
HincII GTYRAC 6 cut(s) 562, 1389, 2095, 2602, 3957, 4053
HindII GTYRAC 6 cut(s) 562, 1389, 2095, 2602, 3957, 4053
HindIII AAGCTT 4 cut(s) 881, 1706, 2207, 3738
HpaI GTTAAC 1 cut(s) 4053
HpaII CCGG 6 cut(s) 236, 501, 589, 607, 3324, 4340
HphI GGTGA 8 cut(s) 55, 190, 751, 757, 2111, 2231, 2720, 3338
HpyCH4IV ACGT 6 cut(s) 684, 1126, 1333, 1374, 2486, 4554
HpySE526I ACGT 6 cut(s) 684, 1126, 1333, 1374, 2486, 4554
HspAI GCGC 2 cut(s) 1978, 2826
Kpn2I TCCGGA 1 cut(s) 4339
Ksp22I TGATCA 3 cut(s) 3127, 4285, 4354
KspAI GTTAAC 1 cut(s) 4053
LguI GCTCTTC 1 cut(s) 2404
Lsp1109I GCAGC 8 cut(s) 648, 1025, 2177, 2677, 2991, 3172, 3679, 3964
MaeII ACGT 6 cut(s) 684, 1126, 1333, 1374, 2486, 4554
MaeIII GTNAC 7 cut(s) 1448, 2099, 2339, 2490, 2783, 3326, 4093
MbiI CCGCTC 1 cut(s) 288
MfeI CAATTG 4 cut(s) 1832, 2880, 3843, 4358
MflI RGATCY 4 cut(s) 17, 1667, 1932, 3341
MhlI GDGCHC 4 cut(s) 658, 971, 2430, 3934
MmeI TCCRAC 3 cut(s) 3798, 4296, 4302
Mph1103I ATGCAT 1 cut(s) 2898
MroI TCCGGA 1 cut(s) 4339
MroXI GAANNNNTTC 1 cut(s) 4232
MslI CAYNNNNRTG 4 cut(s) 1638, 3138, 3165, 3713
MspA1I CMGCKG 2 cut(s) 661, 905
MspCI CTTAAG 1 cut(s) 3992
MspI CCGG 6 cut(s) 236, 501, 589, 607, 3324, 4340
MunI CAATTG 4 cut(s) 1832, 2880, 3843, 4358
Mva1269I GAATGC 7 cut(s) 853, 1465, 1908, 2546, 2588, 3883, 4234
NciI CCSGG 1 cut(s) 608
NlaIV GGNNCC 9 cut(s) 297, 713, 1749, 3112, 3750, 4045, 4294, 4560, 4624
NmeAIII GCCGAG 2 cut(s) 243, 4072
NmuCI GTSAC 6 cut(s) 1448, 2099, 2339, 2490, 2783, 3326
NsiI ATGCAT 1 cut(s) 2898
NspI RCATGY 3 cut(s) 1684, 2995, 4315
PaeR7I CTCGAG 1 cut(s) 3389
PagI TCATGA 3 cut(s) 583, 3514, 3985
PceI AGGCCT 1 cut(s) 1210
PciI ACATGT 1 cut(s) 4311
PciSI GCTCTTC 1 cut(s) 2404
PctI GAATGC 7 cut(s) 853, 1465, 1908, 2546, 2588, 3883, 4234
PdmI GAANNNNTTC 1 cut(s) 4232
PflFI GACNNNGTC 1 cut(s) 3550
PflMI CCANNNNNTGG 2 cut(s) 1664, 1784
PfoI TCCNGGA 2 cut(s) 1179, 4380
PkrI GCNGC 9 cut(s) 663, 1015, 2192, 2692, 2728, 3006, 3187, 3694, 3979
Ppu21I YACGTR 3 cut(s) 685, 2487, 4555
PpuMI RGGWCCY 1 cut(s) 3923
PscI ACATGT 1 cut(s) 4311
Psp124BI GAGCTC 3 cut(s) 658, 971, 2430
Psp5II RGGWCCY 1 cut(s) 3923
PspEI GGTNACC 1 cut(s) 3326
PspFI CCCAGC 2 cut(s) 3115, 4490
PspN4I GGNNCC 9 cut(s) 297, 713, 1749, 3112, 3750, 4045, 4294, 4560, 4624
PspPI GGNCC 9 cut(s) 26, 233, 712, 770, 1073, 1194, 1748, 3923, 4449
PspPPI RGGWCCY 1 cut(s) 3923
PsrI GAACNNNNNNTAC 2 cut(s) 1211, 1243
PstNI CAGNNNCTG 3 cut(s) 1153, 3004, 3977
PsuI RGATCY 4 cut(s) 17, 1667, 1932, 3341
PsyI GACNNNGTC 1 cut(s) 3550
PvuII CAGCTG 2 cut(s) 661, 905
RseI CAYNNNNRTG 4 cut(s) 1638, 3138, 3165, 3713
SacI GAGCTC 3 cut(s) 658, 971, 2430
SapI GCTCTTC 1 cut(s) 2404
SatI GCNGC 9 cut(s) 662, 1014, 2191, 2691, 2727, 3005, 3186, 3693, 3978
Sau96I GGNCC 9 cut(s) 26, 233, 712, 770, 1073, 1194, 1748, 3923, 4449
SduI GDGCHC 4 cut(s) 658, 971, 2430, 3934
SfcI CTRYAG 6 cut(s) 784, 1924, 2674, 2996, 3174, 3639
Sfr274I CTCGAG 1 cut(s) 3389
SinI GGWCC 4 cut(s) 712, 1073, 1194, 3923
SlaI CTCGAG 1 cut(s) 3389
SmiMI CAYNNNNRTG 4 cut(s) 1638, 3138, 3165, 3713
SmlI CTYRAG 5 cut(s) 2930, 3389, 3918, 3992, 4457
SmoI CTYRAG 5 cut(s) 2930, 3389, 3918, 3992, 4457
SpeI ACTAGT 1 cut(s) 3959
SseBI AGGCCT 1 cut(s) 1210
SsiI CCGC 8 cut(s) 288, 328, 442, 951, 2727, 2885, 3753, 4626
SspI AATATT 1 cut(s) 3478
SstI GAGCTC 3 cut(s) 658, 971, 2430
StuI AGGCCT 1 cut(s) 1210
StyI CCWWGG 2 cut(s) 4011, 4263
TaiI ACGT 6 cut(s) 687, 1129, 1336, 1377, 2489, 4557
TatI WGTACW 4 cut(s) 1242, 1718, 2086, 4308
TauI GCSGC 1 cut(s) 2729
TseFI GTSAC 6 cut(s) 1448, 2099, 2339, 2490, 2783, 3326
TseI GCWGC 8 cut(s) 661, 1013, 2190, 2690, 3004, 3185, 3692, 3977
Tsp45I GTSAC 6 cut(s) 1448, 2099, 2339, 2490, 2783, 3326
TspGWI ACGGA 4 cut(s) 1177, 1463, 2623, 4133
Tth111I GACNNNGTC 1 cut(s) 3550
Van91I CCANNNNNTGG 2 cut(s) 1664, 1784
Vha464I CTTAAG 1 cut(s) 3992
VpaK11BI GGWCC 4 cut(s) 712, 1073, 1194, 3923
XagI CCTNNNNNAGG 2 cut(s) 3087, 3894
XapI RAATTY 8 cut(s) 55, 530, 931, 1949, 2437, 3280, 3605, 4519
XbaI TCTAGA 2 cut(s) 3856, 4111
XceI RCATGY 3 cut(s) 1684, 2995, 4315
XcmI CCANNNNNNNNNTGG 1 cut(s) 722
XhoI CTCGAG 1 cut(s) 3389
XmiI GTMKAC 4 cut(s) 550, 3642, 3674, 4166
XmnI GAANNNNTTC 1 cut(s) 4232
Zsp2I ATGCAT 1 cut(s) 2898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.