RchiOBHm_Chr4g0416261

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0007814
Physical Location & Seq
Reverse (-)
41123543 .. 41132887
9345 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38642

Sequence Viewer

Length: 4659 bp
ATGGACGAAGGTGTGAGATCTGTTGGCCCTTCTGGGGTGTTGGTGAAGAATAGGAATTCTTCGGGGTGTTTAATTGTGAGGAAGAAGCCCGATGGGGTTAGTGGTGGCGTTGGTTCTTCGAGTTCTCGGAAGGTTTTCGAATCGAGGAAGGAGAAGAAGAGGTCGAGGTTGGTGATGAGTGATTCAGGGTCGAGTGATGAGCTGTTGATGCCGAGGAGGAAAGTTGGGCCGGAGACTATTCGGGTTTGTAATGGTTTGGACAAGGGCATTGTGGAAGGGAGCGGAATGGTTCCGAAGAGGGAGAGACTGGAGCATGGTAGGCGGAATGAGGATGGTATGATTGGTACAAATTATTTGGATGATGGGGGAGGAAGGAGAACTAATTTGGAGGTGTTTGAGTTCAATGAGTATGATGGGATTGAGGGACAACCGATGCGGAGGAGTCGGTTCGGCGATGGAGTTATCGGAGTTGATTTTGGGGAGAGAAGATATGCGGGTGTACCCCGAGGTGGCATTAAGAGGGAATTTGAGACTGGTTCAAGTAGACATCTTGTTGACAAGAGAAAGAACCTGTATCATGACCGGACAAGTAGCTTGAACCGGGGAGATCGCGCTGATAGGGTTAGGTACGAGATGAGTGGCGATGGAGCTCAGCTGCCCTTATTGAGAGACAAGTACATGGGTGTTTCAGATGAACCCATTAGGGTCCAGGGTAAAAATGGTGTTTTGAAGGTGATGGTGAAGAAGAAAAACAATGTGCCTGGGCCACTAGGGAACTATAGTTTTCCAAAATCTGAAGAACATAGAAAAGCTCCAAGAAGTGAGGATATTTCCAAGAAGAATGCTATCATCCCTCCATTTTTCGCAGAACCAAAGCTTCCTGAGAAACCTGTTTCAGCTGTTAGGACAGAGAAAAACCATGTGAATTTGCGAAAATCATTGCCCACTAAGAACAGCAAGAGCTCTGACTGGGATTCGGAGGACAGTGATACCTCATTGAAGCTAGCAGCAAAGAGTGCCGAAGCCTCTAAACCTATGAAGAGGGCAGGTTGCAAAGTTGAAGATGGTCCTTCATCTGAAAAGACCCCTCCAGCCAGAAATAAAGAAGTCAAACTTAAACGTGGTAGTGGCACAGAGAAGCAGAAACTGCGTGAACGGATAAGGGAGATGCTCCTGAATGCAGGCTGGACCATAGATTATAGGCCTAGAAGGAACAGAGACTACCTGGATGCTGTGTACATTAACCCATCAGGTACAGCTTACTGGTCTATCATTAAGGCTTATGACGCACTTCAAAAGCAAATAAATGAGGAAAATGAGGCTAAACGTAATGGGGATGGTTCTTCACTTGCACCTATAACTGATGATGTACTCAGTCAGTTAACCAGGAAAACTCGAAAGAAGATGGAAAAAGAAATGAAAAGGAAGCAAAGAGCTGACAGTGACAGTGAGAATGCAAAAGGAGTACGTGTGAAAAAAACTAAAAGTGCCAAGCATGATCCAGAGAGCATGGACAGTGTTAGTTATGAGGAGAAACTAAGCTCCTACTTGAAGCAAGGTGGTAAGTCATTCAAGGGTAGAATGTATGAAAACGGTTTGGATTCAAATGGTCAGAGTTCCTCTCATCATTTACATGGCACTGTTGAAAAACCATCTTCTGGATCTAGTTCTCATATGCCACATGGAAGGAAAAGTAGAAAGCTTGGTAGATGTACTTTGTTGGTTCGTAGTTCTAAAAAGGGGCCTAACTCGGAAAGTGATGGCTTTGTTCCATATACTGGAAAACGAACATTGCTCTCCTGGCTGATTGATACTGGAACTGTGCAATTGAGCCAAAAGGTGCAGTATATGAATCGGCGAAGAACTAAAGTAATGCTGGAGGGCTGGATTACAAGAGATGGCATTCACTGTGGTTGCTGTAGTAAGATCCTCACAGTCTCGAAATTTGAGATTCATGCAGGGAGCAAATTGCGCCAGCCATTTCAAAATATATGCTTGGACTCTGGGGTTTCCCTTCTGCAATGTCAGATAGATGCATGGAATAGACAAGAGGATATTGAACGCATTGGTTTCCACTCTGTACAAGTTGACGGTGACGATCCCGATGATGATACTTGTGGCCTCTGTGGAGATGGTGGGGATTTGATCTGTTGTGATGGCTGTCCTTCAACATTTCATCAGAGCTGCTTGAACATACAGAAGCTTCCTCCAGGTGATTGGCACTGCCCAAATTGTATTTGCAAAGTTTGTGGAATAGCTAGTGAGGTTGTTGCTGAAGAGGATGATGCAACTGTTTCTGCACTACTGGCTTGTAGTCTCTGTGGGAAGAAATGTCACATATCATGCAGTCAGGAGATGGATGCTAGTCCTGCTGATTCCAATAGTTCAGGCTCTTCCTTTTGTGGGCAGAGATGTAGAGAGCTCTTTGAGAATTTGCAGAGATGTCTCGGGGTCAAACATGAACTAGAAGCAGGATATACGTGGTCACTTGTAAAGAGAACAGATGTAGATAGAGGATTTCCTCTGAGGGTTGAATGCAATTCTAAGCTCGCTGTTGCATTGACTGTTATGGATGAATGCTTTTTGCCTATTGTTGACAGACGGAGTGGGATCAATTTAATCCATAATGTTCTATATAATTGTGGATCAAACTTCAATCGGCTGAACTACAGTGGCTTCTACGCTGCTATTTTGGAGAAAGGTGATGAAATAATATCTGCCGCTTCTATCAGGTTTCATGGTACAATGTTAGCAGAGATGCCATTCATCGGAACTCGTCACATATACAGGCGACAAGGGATGTGTCGTAGGCTTTTCTGCGCCATTGAATCGGCTCTCTGCTCCCTGAAGGTTGAGAAGTTGGTTATCCCTGCAATTGCGGAACTCATGCATACATGGACGGGTGTTTTTGGTTTTGTTCCTCTTGAGGAATCATTCAAGCAAGAAGTGAGGTCAATCAATATGTTAGTCTTCCCTGGTATAGACATGCTACAGAAGCTGCTGGTGGACAAAGAAAATGAGACAAACATGACAGGTGTAAAGAAAATGGACGGCATAGGCAAGGATTGTATAAAGCCTGGAGGAGGAGGCAAATCTGATATTGGTTCCCCAGCTAGTCTTGATCATCATGGGAGTGATGGAGCTGGTATGCTTCATATCAATGAAATTGTAGATGAAGCTGCTGATGTGGACTCTGGTTCTCGATGTCTAGGTGTCTCCTTAAATGATACTCCTGTAATGAGTGGCTCTTTGGATGCCTCTGATGAACTCAAAAATTTAGATTCAACTGGAAGGAGTATGTCTTCCGAGTCTCCTGCCGGTGACCAATTAGCTGGATCTACTTCTGACGGGAAATGCCTTCCTAATACAAGCCATGAAGTTCTCGAGATAGGAACTAAACCACTGCTGGAGTCTCCTGTTGAGGATAATATGCAGTGTAATTCCAAGTGTCCTATTGTTTCTTCAAATAATATTTCCATGTTGAATGGTGGTTCATTAGATGCTTCTCATGAACAAAACAATCAGATTTCGATCAATGGGACTCTGTCTGCTGACTCTGATTCAGGTGCTAAGTTAGCTGAATATGGTTCTGAAGCAAAATTCCCATCTCCCCTTGAAAGAAGCAGTGAAGCTGTAGACACTGCGCCTGTTGAAGATAATGTCTTGTCTACCTCGCAGTGTCCTGCTGCCTCATTAAATAATACTTCCATCATAAGGAGTAATCTAATGGAAGCTTCTCAGGTTCCCGCTAAGGGGACTGCATCTTCCGACTCCAATTCAGTTGCCAAGTTAGATGAATACACTTCTGAAGCAAAATGCCAGTCTCCCTCTAAAACAATTGATGAAGCTTTAGAGATTGGAAATGAAACAATACTGCATTCTCCAGTTGAAGTTAATATGCAGTCCACCTCTCAAGGTCCTGGTGCTCCATTAAATAATACTTCCATGTTGACTAGTAGCTCATCAGATGCTGCTTCTCATGAACTTAAGGTTTTGGATTCAACCAAGGGGAATCCATCTTTTGACTGTGATTCAGGTGCCGAGTTAACTGAACACACTTCTGAAGTGAAACGCAAGTCTTCCCCTGTTACAAGACATGAAGTTCTAGAGAAGGAAAATCAACCAGTGGCAGATTCTCCCATTGAAGATAAGATCCAGTCTACCTCTCAGAGTCCTGATGCTTCGTTAAAGGATACTTCCATGTTTAGTAGTGGTTCATTGAATGTTTTCCATGAACTCAACATTCAGCCTTCAGCCAAGGGGACTCCAGCCAACTCTGATCAAGGCGCCAAGTTAGCCGAGTACACGTTGGATGCAAAATGTCAGTCTTCTCCGGATACAAGTGATCATCAAATGGTGGGAGTAAAAACATTCCTGGATAATTCTTCAAGGTGCTTGAAAGAAAGTGCTTCTGATAATTCCCAAGAAGAGAATGTTGACAGGGCCTCCCTTGAGCCTGTTCCTTTTTCAGGAGAAACTTTTGCTGGGAACAGTGAGGAAGATGGAAATCCAAATTCGAGTTTCTGTGAGACTGAGAAACAATCATACGTGGCTCCAGAAGTTGCTTCTGATGCAGTGCATTGTGAAAAGAGCATCCCTAAAGCATCTAGGGATGGCTCCGCTACTGATTCTGGGAGGGATGAATCTGGTTCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005700 GO:0006323 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006996 GO:0007063 GO:0007276 GO:0007283 GO:0008150 GO:0008270 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010639 GO:0010948 GO:0016043 GO:0016462 GO:0016581 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019219 GO:0019222 GO:0019953 GO:0022414 GO:0030261 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031491 GO:0031974 GO:0031981 GO:0032501 GO:0032504 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0034728 GO:0035327 GO:0042623 GO:0042766 GO:0043044 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044703 GO:0044877 GO:0045786 GO:0045875 GO:0045892 GO:0045934 GO:0046872 GO:0046914 GO:0048232 GO:0048519 GO:0048523 GO:0048609 GO:0050789 GO:0050794 GO:0051098 GO:0051101 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051312 GO:0051704 GO:0051726 GO:0051983 GO:0051985 GO:0060255 GO:0060341 GO:0065007 GO:0065009 GO:0070013 GO:0070603 GO:0070615 GO:0071103 GO:0071824 GO:0071840 GO:0071922 GO:0071923 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:1901363 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1903827 GO:1903828 GO:1904949 GO:1905634 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas PHD

Protein Analysis

1552

Amino Acids

169.03

Weight (kDa)

6.21

Isoelectric Point (pI)

51.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7028 PF22970 371 - 434 7.3e-15 Domain of unknown function (DUF7028)
TDBD PF16135 628 - 678 2.6e-21 Tify domain binding domain
PHD PF00628 706 - 745 4.6e-07 PHD-finger
IDM1_C PF23209 861 - 999 2.5e-58 Increased DNA methylation 1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1037
AccB1I GGYRCC 2 cut(s) 4037, 4286
AccB7I CCANNNNNTGG 2 cut(s) 1658, 1778
AccBSI CCGCTC 1 cut(s) 282
AccI GTMKAC 4 cut(s) 544, 3636, 3668, 4160
AccII CGCG 1 cut(s) 612
AccIII TCCGGA 1 cut(s) 4333
AciI CCGC 8 cut(s) 282, 322, 436, 494, 2721, 2879, 3747, 4620
AclWI GGATC 8 cut(s) 1493, 1669, 1921, 2093, 2618, 2653, 3343, 4147
AcsI RAATTY 8 cut(s) 55, 524, 925, 1943, 2431, 3274, 3599, 4513
AcuI CTGAAG 7 cut(s) 816, 2295, 2867, 3612, 3828, 4083, 4236
AcyI GRCGYC 1 cut(s) 4287
AflII CTTAAG 1 cut(s) 3986
AflIII ACRYGT 2 cut(s) 1468, 4305
AhlI ACTAGT 1 cut(s) 3953
Alw21I GWGCWC 4 cut(s) 652, 965, 2424, 3928
AlwI GGATC 8 cut(s) 1493, 1669, 1921, 2093, 2618, 2653, 3343, 4147
AlwNI CAGNNNCTG 3 cut(s) 1147, 2998, 3971
Ama87I CYCGRG 3 cut(s) 504, 2447, 3383
Aor13HI TCCGGA 1 cut(s) 4333
AoxI GGCC 7 cut(s) 25, 227, 764, 1202, 1742, 2119, 4443
ApeKI GCWGC 8 cut(s) 655, 1007, 2184, 2684, 2998, 3179, 3686, 3971
ApoI RAATTY 8 cut(s) 55, 524, 925, 1943, 2431, 3274, 3599, 4513
ArsI GACNNNNNNTTYG 4 cut(s) 3806, 3838, 4310, 4342
Asp700I GAANNNNTTC 2 cut(s) 134, 4226
AspLEI GCGC 5 cut(s) 614, 1974, 2822, 3646, 4289
AspS9I GGNCC 9 cut(s) 26, 227, 706, 764, 1067, 1188, 1742, 3917, 4443
AsuC2I CCSGG 1 cut(s) 602
AsuHPI GGTGA 8 cut(s) 55, 184, 745, 751, 2105, 2225, 2714, 3332
AsuII TTCGAA 1 cut(s) 138
AsuNHI GCTAGC 1 cut(s) 1003
AvaI CYCGRG 3 cut(s) 504, 2447, 3383
AvaII GGWCC 4 cut(s) 706, 1067, 1188, 3917
BaeI ACNNNNGTAYC 2 cut(s) 3219, 3252
BanI GGYRCC 2 cut(s) 4037, 4286
BanII GRGCYC 3 cut(s) 652, 965, 2424
BbsI GAAGAC 4 cut(s) 2962, 3294, 4071, 4320
Bbv12I GWGCWC 4 cut(s) 652, 965, 2424, 3928
BbvI GCAGC 8 cut(s) 642, 1019, 2171, 2671, 2985, 3166, 3673, 3958
BceAI ACGGC 1 cut(s) 3067
BcgI CGANNNNNNTGC 2 cut(s) 3296, 3330
BciVI GTATCC 2 cut(s) 4186, 4330
BclI TGATCA 3 cut(s) 3121, 4279, 4345
BcnI CCSGG 1 cut(s) 602
BcuI ACTAGT 1 cut(s) 3953
BfmI CTRYAG 5 cut(s) 778, 1918, 2668, 2990, 3633
BfoI RGCGCY 1 cut(s) 4290
BfrI CTTAAG 1 cut(s) 3986
BfuAI ACCTGC 1 cut(s) 1037
BfuI GTATCC 2 cut(s) 4186, 4330
BglII AGATCT 1 cut(s) 17
BisI GCNGC 9 cut(s) 656, 1008, 2185, 2685, 2721, 2999, 3180, 3687, 3972
BlpI GCTNAGC 1 cut(s) 651
BlsI GCNGC 9 cut(s) 657, 1009, 2186, 2686, 2722, 3000, 3181, 3688, 3973
Bme18I GGWCC 4 cut(s) 706, 1067, 1188, 3917
BmeT110I CYCGRG 3 cut(s) 504, 2447, 3383
BmgT120I GGNCC 9 cut(s) 26, 227, 706, 764, 1067, 1188, 1742, 3917, 4443
BmiI GGNNCC 9 cut(s) 291, 707, 1743, 3106, 3744, 4039, 4288, 4554, 4618
BmrI ACTGGG 1 cut(s) 979
BmtI GCTAGC 1 cut(s) 1007
BmuI ACTGGG 1 cut(s) 979
BpiI GAAGAC 4 cut(s) 2962, 3294, 4071, 4320
BplI GAGNNNNNCTC 2 cut(s) 1606, 1638
BpmI CTGGAG 9 cut(s) 329, 1074, 1898, 2193, 3099, 3428, 3867, 4251, 4539
Bpu10I CCTNAGC 1 cut(s) 3750
Bpu1102I GCTNAGC 1 cut(s) 651
Bpu14I TTCGAA 1 cut(s) 138
BpuEI CTTGAG 3 cut(s) 2945, 3897, 4472
BpuMI CCSGG 1 cut(s) 602
BsaAI YACGTR 3 cut(s) 1469, 2481, 4549
BsaBI GATNNNNATC 2 cut(s) 3530, 4506
BsaHI GRCGYC 1 cut(s) 4287
BsaJI CCNNGG 8 cut(s) 212, 505, 601, 709, 761, 2974, 4005, 4257
BsaWI WCCGGW 2 cut(s) 582, 4333
BsaXI ACNNNNNCTCC 4 cut(s) 1455, 1485, 4430, 4460
Bse118I RCCGGY 1 cut(s) 3317
Bse3DI GCAATG 3 cut(s) 938, 1790, 2027
Bse8I GATNNNNATC 2 cut(s) 3530, 4506
BseAI TCCGGA 1 cut(s) 4333
BseDI CCNNGG 8 cut(s) 212, 505, 601, 709, 761, 2974, 4005, 4257
BseJI GATNNNNATC 2 cut(s) 3530, 4506
BseMI GCAATG 3 cut(s) 938, 1790, 2027
BseMII CTCAG 7 cut(s) 665, 873, 1387, 2516, 3752, 4181, 4524
BseRI GAGGAG 5 cut(s) 229, 454, 1544, 3096, 3099
BseXI GCAGC 8 cut(s) 642, 1019, 2171, 2671, 2985, 3166, 3673, 3958
BseYI CCCAGC 2 cut(s) 3109, 4484
BsgI GTGCAG 2 cut(s) 1862, 2283
Bsh1236I CGCG 1 cut(s) 612
BshFI GGCC 7 cut(s) 27, 229, 766, 1204, 1744, 2121, 4445
BshNI GGYRCC 2 cut(s) 4037, 4286
BsiHKAI GWGCWC 4 cut(s) 652, 965, 2424, 3928
BsiHKCI CYCGRG 3 cut(s) 504, 2447, 3383
BsiSI CCGG 5 cut(s) 230, 583, 601, 3318, 4334
BslFI GGGAC 4 cut(s) 438, 3553, 3769, 4276
BsmFI GGGAC 4 cut(s) 438, 3553, 3769, 4276
BsmI GAATGC 7 cut(s) 847, 1183, 1459, 1902, 2540, 2582, 3877
BsnI GGCC 7 cut(s) 27, 229, 766, 1204, 1744, 2121, 4445
BsoBI CYCGRG 3 cut(s) 504, 2447, 3383
Bsp119I TTCGAA 1 cut(s) 138
Bsp1286I GDGCHC 4 cut(s) 652, 965, 2424, 3928
Bsp13I TCCGGA 1 cut(s) 4333
Bsp1407I TGTACA 2 cut(s) 1236, 2080
Bsp1720I GCTNAGC 1 cut(s) 651
BspACI CCGC 8 cut(s) 282, 322, 436, 494, 2721, 2879, 3747, 4620
BspANI GGCC 7 cut(s) 27, 229, 766, 1204, 1744, 2121, 4445
BspCNI CTCAG 7 cut(s) 664, 874, 1386, 2517, 3751, 4180, 4525
BspEI TCCGGA 1 cut(s) 4333
BspFNI CGCG 1 cut(s) 612
BspHI TCATGA 3 cut(s) 577, 3508, 3979
BspLI GGNNCC 9 cut(s) 291, 707, 1743, 3106, 3744, 4039, 4288, 4554, 4618
BspMI ACCTGC 1 cut(s) 1037
BspOI GCTAGC 1 cut(s) 1007
BspPI GGATC 8 cut(s) 1493, 1669, 1921, 2093, 2618, 2653, 3343, 4147
BspQI GCTCTTC 1 cut(s) 2398
BspT104I TTCGAA 1 cut(s) 138
BspT107I GGYRCC 2 cut(s) 4037, 4286
BspTI CTTAAG 1 cut(s) 3986
BsrBI CCGCTC 1 cut(s) 282
BsrDI GCAATG 3 cut(s) 938, 1790, 2027
BsrFI RCCGGY 1 cut(s) 3317
BsrGI TGTACA 2 cut(s) 1236, 2080
BssAI RCCGGY 1 cut(s) 3317
BssECI CCNNGG 8 cut(s) 212, 505, 601, 709, 761, 2974, 4005, 4257
BssNI GRCGYC 1 cut(s) 4287
BssT1I CCWWGG 2 cut(s) 4005, 4257
Bst6I CTCTTC 6 cut(s) 152, 290, 1034, 2271, 2398, 4422
BstACI GRCGYC 1 cut(s) 4287
BstAFI CTTAAG 1 cut(s) 3986
BstAPI GCANNNNNTGC 2 cut(s) 1016, 1147
BstAUI TGTACA 2 cut(s) 1236, 2080
BstBAI YACGTR 3 cut(s) 1469, 2481, 4549
BstBI TTCGAA 1 cut(s) 138
BstC8I GCNNGC 4 cut(s) 1005, 1183, 1976, 2550
BstEII GGTNACC 1 cut(s) 3320
BstENI CCTNNNNNAGG 2 cut(s) 3081, 4467
BstFNI CGCG 1 cut(s) 612
BstH2I RGCGCY 1 cut(s) 4290
BstHHI GCGC 5 cut(s) 614, 1974, 2822, 3646, 4289
BstNSI RCATGY 1 cut(s) 2989
BstPI GGTNACC 1 cut(s) 3320
BstSFI CTRYAG 5 cut(s) 778, 1918, 2668, 2990, 3633
BstUI CGCG 1 cut(s) 612
BstV1I GCAGC 8 cut(s) 642, 1019, 2171, 2671, 2985, 3166, 3673, 3958
BstV2I GAAGAC 4 cut(s) 2962, 3294, 4071, 4320
BstX2I RGATCY 5 cut(s) 17, 1661, 1926, 3335, 4152
BstXI CCANNNNNNTGG 2 cut(s) 2217, 3332
BstYI RGATCY 5 cut(s) 17, 1661, 1926, 3335, 4152
BsuI GTATCC 2 cut(s) 4186, 4330
BsuRI GGCC 7 cut(s) 27, 229, 766, 1204, 1744, 2121, 4445
BtgZI GCGATG 2 cut(s) 468, 657
BtsI GCAGTG 7 cut(s) 2221, 3401, 3440, 3631, 3639, 3683, 4581
BveI ACCTGC 1 cut(s) 1037
Cac8I GCNNGC 4 cut(s) 1005, 1183, 1976, 2550
CaiI CAGNNNCTG 3 cut(s) 1147, 2998, 3971
CciI TCATGA 3 cut(s) 577, 3508, 3979
CfoI GCGC 5 cut(s) 614, 1974, 2822, 3646, 4289
Cfr10I RCCGGY 1 cut(s) 3317
Cfr13I GGNCC 9 cut(s) 26, 227, 706, 764, 1067, 1188, 1742, 3917, 4443
CseI GACGC 1 cut(s) 1295
CspCI CAANNNNNGTGG 2 cut(s) 2230, 2265
DinI GGCGCC 1 cut(s) 4288
Eam1104I CTCTTC 6 cut(s) 152, 290, 1034, 2271, 2398, 4422
EarI CTCTTC 6 cut(s) 152, 290, 1034, 2271, 2398, 4422
EciI GGCGGA 1 cut(s) 337
Ecl136II GAGCTC 3 cut(s) 650, 963, 2422
Eco130I CCWWGG 2 cut(s) 4005, 4257
Eco147I AGGCCT 1 cut(s) 1204
Eco24I GRGCYC 3 cut(s) 652, 965, 2424
Eco47I GGWCC 4 cut(s) 706, 1067, 1188, 3917
Eco53kI GAGCTC 3 cut(s) 650, 963, 2422
Eco57I CTGAAG 7 cut(s) 816, 2295, 2867, 3612, 3828, 4083, 4236
Eco88I CYCGRG 3 cut(s) 504, 2447, 3383
Eco91I GGTNACC 1 cut(s) 3320
EcoICRI GAGCTC 3 cut(s) 650, 963, 2422
EcoNI CCTNNNNNAGG 2 cut(s) 3081, 4467
EcoO109I RGGNCCY 3 cut(s) 1742, 3917, 4443
EcoO65I GGTNACC 1 cut(s) 3320
EcoRI GAATTC 1 cut(s) 55
EcoT14I CCWWGG 2 cut(s) 4005, 4257
EcoT22I ATGCAT 2 cut(s) 2038, 2892
EcoT38I GRGCYC 3 cut(s) 652, 965, 2424
EgeI GGCGCC 1 cut(s) 4288
EheI GGCGCC 1 cut(s) 4288
ErhI CCWWGG 2 cut(s) 4005, 4257
FalI AAGNNNNNCTT 2 cut(s) 1098, 1130
FaqI GGGAC 4 cut(s) 438, 3553, 3769, 4276
FauI CCCGC 2 cut(s) 487, 3754
FauNDI CATATG 1 cut(s) 1674
FbaI TGATCA 3 cut(s) 3121, 4279, 4345
FblI GTMKAC 4 cut(s) 544, 3636, 3668, 4160
Fnu4HI GCNGC 9 cut(s) 656, 1008, 2185, 2685, 2721, 2999, 3180, 3687, 3972
FriOI GRGCYC 3 cut(s) 652, 965, 2424
Fsp4HI GCNGC 9 cut(s) 656, 1008, 2185, 2685, 2721, 2999, 3180, 3687, 3972
GlaI GCGC 5 cut(s) 613, 1973, 2821, 3645, 4288
GluI GCNGC 9 cut(s) 656, 1008, 2185, 2685, 2721, 2999, 3180, 3687, 3972
GsaI CCCAGC 2 cut(s) 3113, 4488
GsuI CTGGAG 9 cut(s) 329, 1074, 1898, 2193, 3099, 3428, 3867, 4251, 4539
HaeII RGCGCY 1 cut(s) 4290
HaeIII GGCC 7 cut(s) 27, 229, 766, 1204, 1744, 2121, 4445
HapII CCGG 5 cut(s) 230, 583, 601, 3318, 4334
HgaI GACGC 1 cut(s) 1295
HhaI GCGC 5 cut(s) 614, 1974, 2822, 3646, 4289
Hin1I GRCGYC 1 cut(s) 4287
Hin6I GCGC 5 cut(s) 612, 1972, 2820, 3644, 4287
HinP1I GCGC 5 cut(s) 612, 1972, 2820, 3644, 4287
HincII GTYRAC 7 cut(s) 556, 1383, 2089, 2596, 3951, 4047, 4438
HindII GTYRAC 7 cut(s) 556, 1383, 2089, 2596, 3951, 4047, 4438
HindIII AAGCTT 5 cut(s) 875, 1700, 2201, 3732, 3846
HpaI GTTAAC 2 cut(s) 1383, 4047
HpaII CCGG 5 cut(s) 230, 583, 601, 3318, 4334
HphI GGTGA 8 cut(s) 55, 184, 745, 751, 2105, 2225, 2714, 3332
HpyCH4IV ACGT 6 cut(s) 1120, 1327, 1468, 2480, 4307, 4548
HpySE526I ACGT 6 cut(s) 1120, 1327, 1468, 2480, 4307, 4548
Hsp92I GRCGYC 1 cut(s) 4287
HspAI GCGC 5 cut(s) 612, 1972, 2820, 3644, 4287
KasI GGCGCC 1 cut(s) 4286
Kpn2I TCCGGA 1 cut(s) 4333
Ksp22I TGATCA 3 cut(s) 3121, 4279, 4345
KspAI GTTAAC 2 cut(s) 1383, 4047
LguI GCTCTTC 1 cut(s) 2398
Lsp1109I GCAGC 8 cut(s) 642, 1019, 2171, 2671, 2985, 3166, 3673, 3958
MaeII ACGT 6 cut(s) 1120, 1327, 1468, 2480, 4307, 4548
MaeIII GTNAC 7 cut(s) 1442, 2093, 2333, 2484, 2777, 3320, 4087
MbiI CCGCTC 1 cut(s) 282
MfeI CAATTG 3 cut(s) 1826, 2874, 3837
MflI RGATCY 5 cut(s) 17, 1661, 1926, 3335, 4152
MhlI GDGCHC 4 cut(s) 652, 965, 2424, 3928
Mly113I GGCGCC 1 cut(s) 4287
MmeI TCCRAC 2 cut(s) 3792, 4290
Mph1103I ATGCAT 2 cut(s) 2038, 2892
MroI TCCGGA 1 cut(s) 4333
MroXI GAANNNNTTC 2 cut(s) 134, 4226
MslI CAYNNNNRTG 3 cut(s) 1632, 3132, 3159
MspA1I CMGCKG 2 cut(s) 655, 899
MspCI CTTAAG 1 cut(s) 3986
MspI CCGG 5 cut(s) 230, 583, 601, 3318, 4334
MunI CAATTG 3 cut(s) 1826, 2874, 3837
Mva1269I GAATGC 7 cut(s) 847, 1183, 1459, 1902, 2540, 2582, 3877
MvnI CGCG 1 cut(s) 612
NarI GGCGCC 1 cut(s) 4287
NciI CCSGG 1 cut(s) 602
NdeI CATATG 1 cut(s) 1674
NheI GCTAGC 1 cut(s) 1003
NlaIV GGNNCC 9 cut(s) 291, 707, 1743, 3106, 3744, 4039, 4288, 4554, 4618
NmeAIII GCCGAG 3 cut(s) 237, 4066, 4324
NmuCI GTSAC 6 cut(s) 1442, 2093, 2333, 2484, 2777, 3320
NsiI ATGCAT 2 cut(s) 2038, 2892
NspI RCATGY 1 cut(s) 2989
NspV TTCGAA 1 cut(s) 138
PaeR7I CTCGAG 1 cut(s) 3383
PagI TCATGA 3 cut(s) 577, 3508, 3979
PceI AGGCCT 1 cut(s) 1204
PciSI GCTCTTC 1 cut(s) 2398
PctI GAATGC 7 cut(s) 847, 1183, 1459, 1902, 2540, 2582, 3877
PdmI GAANNNNTTC 2 cut(s) 134, 4226
PflFI GACNNNGTC 1 cut(s) 3544
PflMI CCANNNNNTGG 2 cut(s) 1658, 1778
PfoI TCCNGGA 1 cut(s) 4374
PkrI GCNGC 9 cut(s) 657, 1009, 2186, 2686, 2722, 3000, 3181, 3688, 3973
PluTI GGCGCC 1 cut(s) 4290
Ppu21I YACGTR 3 cut(s) 1469, 2481, 4549
PpuMI RGGWCCY 1 cut(s) 3917
Psp124BI GAGCTC 3 cut(s) 652, 965, 2424
Psp5II RGGWCCY 1 cut(s) 3917
PspEI GGTNACC 1 cut(s) 3320
PspFI CCCAGC 2 cut(s) 3109, 4484
PspN4I GGNNCC 9 cut(s) 291, 707, 1743, 3106, 3744, 4039, 4288, 4554, 4618
PspPI GGNCC 9 cut(s) 26, 227, 706, 764, 1067, 1188, 1742, 3917, 4443
PspPPI RGGWCCY 1 cut(s) 3917
PsrI GAACNNNNNNTAC 2 cut(s) 1205, 1237
PstNI CAGNNNCTG 3 cut(s) 1147, 2998, 3971
PsuI RGATCY 5 cut(s) 17, 1661, 1926, 3335, 4152
PsyI GACNNNGTC 1 cut(s) 3544
PvuII CAGCTG 2 cut(s) 655, 899
RseI CAYNNNNRTG 3 cut(s) 1632, 3132, 3159
SacI GAGCTC 3 cut(s) 652, 965, 2424
SapI GCTCTTC 1 cut(s) 2398
SatI GCNGC 9 cut(s) 656, 1008, 2185, 2685, 2721, 2999, 3180, 3687, 3972
Sau96I GGNCC 9 cut(s) 26, 227, 706, 764, 1067, 1188, 1742, 3917, 4443
SduI GDGCHC 4 cut(s) 652, 965, 2424, 3928
SfcI CTRYAG 5 cut(s) 778, 1918, 2668, 2990, 3633
SfoI GGCGCC 1 cut(s) 4288
Sfr274I CTCGAG 1 cut(s) 3383
SfuI TTCGAA 1 cut(s) 138
SinI GGWCC 4 cut(s) 706, 1067, 1188, 3917
SlaI CTCGAG 1 cut(s) 3383
SmiMI CAYNNNNRTG 3 cut(s) 1632, 3132, 3159
SmlI CTYRAG 5 cut(s) 2924, 3383, 3912, 3986, 4451
SmoI CTYRAG 5 cut(s) 2924, 3383, 3912, 3986, 4451
SpeI ACTAGT 1 cut(s) 3953
SseBI AGGCCT 1 cut(s) 1204
SsiI CCGC 8 cut(s) 282, 322, 436, 494, 2721, 2879, 3747, 4620
SspDI GGCGCC 1 cut(s) 4286
SspI AATATT 1 cut(s) 3472
SstI GAGCTC 3 cut(s) 652, 965, 2424
StuI AGGCCT 1 cut(s) 1204
StyI CCWWGG 2 cut(s) 4005, 4257
TaiI ACGT 6 cut(s) 1123, 1330, 1471, 2483, 4310, 4551
TatI WGTACW 6 cut(s) 675, 1236, 1369, 1712, 2080, 4302
TauI GCSGC 1 cut(s) 2723
TseFI GTSAC 6 cut(s) 1442, 2093, 2333, 2484, 2777, 3320
TseI GCWGC 8 cut(s) 655, 1007, 2184, 2684, 2998, 3179, 3686, 3971
Tsp45I GTSAC 6 cut(s) 1442, 2093, 2333, 2484, 2777, 3320
TspGWI ACGGA 2 cut(s) 1171, 2617
Tth111I GACNNNGTC 1 cut(s) 3544
Van91I CCANNNNNTGG 2 cut(s) 1658, 1778
Vha464I CTTAAG 1 cut(s) 3986
VpaK11BI GGWCC 4 cut(s) 706, 1067, 1188, 3917
XagI CCTNNNNNAGG 2 cut(s) 3081, 4467
XapI RAATTY 8 cut(s) 55, 524, 925, 1943, 2431, 3274, 3599, 4513
XbaI TCTAGA 1 cut(s) 4105
XceI RCATGY 1 cut(s) 2989
XcmI CCANNNNNNNNNTGG 1 cut(s) 716
XhoI CTCGAG 1 cut(s) 3383
XmiI GTMKAC 4 cut(s) 544, 3636, 3668, 4160
XmnI GAANNNNTTC 2 cut(s) 134, 4226
Zsp2I ATGCAT 2 cut(s) 2038, 2892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.