pycom16g16310

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007814
Physical Location & Seq
Forward (+)
12255957 .. 12264289
8333 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g16310.8

Sequence Viewer

Length: 4395 bp
ATGGACGAAGGTGTGAGATCTGTTGGCCCTTCTGGGGTTTTGGTGAAGAAAAATTCTTCAGGTTGCTTAATTGTTAGGAAGAAACCGGAGAAATTGAGTGGCGCTGTTGGTCCTTCGAGTGCTCGTAAGGTTTTCGAGCCGAAAGAGAAGAAGAGGTCGAGGTTGGTATTGAGCGAATCGGGGTCTAGTGATGAGATATTGGCTCCTCCTCGTAGAAAGGTGGGACCTGAAACTATACGGGTTTGCAATGGTTTAAGAGCTGTAGATAAGGGCATTGCTGAAAATAGTGAGTTTGGTCAAAAGCGGGAGAGATTGGATCCGGTTAGGCGTAGTGAGGATGGCATGCTTGGTAAAAGTTACTTGGATGAGAGTGGTGGAAAGAGAAGTAAGTTGGAGGTGTATGAATTTGATGAGTATGATGAGGAGATTTCGAGAAGGAAACGGTTCAGTGATGGGGTAGGTGAGTTTGGGGGAAGAAGGTTTTCAGGAGCGATTCCTGTGCCACTAAGTGGCATTAAGAGGGAATGCGAAACTGGTTCAAGTAGACATGTAGTTGACAAGAGGAAGAATTTGTACTTTGACAGGACGAGTAGCTTGAACCGAGGAGATCATACTGACAGGCGAAGGTTTGAGATGAATAGGGATGGAGCTCAGCTACCCTTATTGAGAGACAAGTTCACGGGTCAATCAGAGGAATCCATTAGGCTACAGGGTAAAAATGGTGTTTTGAAGGTCATGGTGAAGAAGAAAAAGAATGCGGGTGGGCCACTGGAGAATTTTCGCAAATCTGAAGAAAATAGAAAGGCTCTGAGGAGTGTGGATATTGTGAAAAATGCTATTATCCCTCCTTTTTACTCGGAACCAAAGCTTCCTGAGAAACCAGTTTCAGTTGTTAGGCCATTGAAAAACCATCCGAATGTACGAAAATCATTGCCGTCTTTGAACAGTAAAGGCTCCGATTGGGATTCAGAGGATAGTGATACATCACTGAAACGAGGACCAAAGAGTGTCAAAGCCTCTAAACCTCGGAAGAGGGTGGTTTGCAAAGATGAAGGTGGTCCTTCATGTGAAAAGACTCCTCCAACCAGAATCAAAGAAGGTAAAGTTAAACGTGGTAGTGGCACGGAGAAGCAAAAACTACGTGAACGAATACGGGAGATGCTGCTGAGTGCAGGTTGGACAATAGATTATAGGCCTAGAAGGAACAGAGACTATCTAGATGCTGTGTACATTAACCCAGCAGGTACAGCTTACTGGTCCATCATTAAGGCTTATGATGCGCTTCAAAAGCAGTTGGATGAGGAGGAAAATGAGGCTAAACCTAGTGGGGTTTCACCTATAACTGATGATATACTTAGCCAATTAACAAGGAAAACTCGAAAGAAGATTGAGAAAGAAATGAAAAGGAAACAAAGGGTTGATGATGAGGATGAGAATGTGAGAGGAGTCCGTATGAAAAGATCGACAAGTATCAAGCGTGATCAAGGGAGCGTGGATAGTGTTAGTTATGAGGATAAACTAAGCACCTACTTAAAGCAGGGTGGTAAGACGTTTAAGAGTGAAAATGGTTTGGCCAGTGTGAACTCAAATCATCTACATGAAGGTATTGAAAAGCCGTCTTCAGGATCAAGTTCGCATATGCCACATGGAAGGAAAAGTAGAAAGCTTGGAAGGTGTACTTTGTTGGTTCGTGGTTCAAAATATGGGGTTAACTCCGAAAGTGATGGCTTTGTTCCATATACTGGGAAACGGACTTTGCTTTCCTGGCTGGTTGACTCTGGAACTGTGCAGTTGAGCCAAAAGGTGCAGTATATGAATCGCCGAAGGACTAAAGTAATGCTGGAGGGGTGGATTACAAGAGATGGCATTCACTGTGGTTGCTGTAGTAAAATCCTCACAACTTCAAAATTTGAGATTCATGCAGGAAGCAAGTTGCGCCAGCCATTTCAAAATATATGTTTGGACTCAGGTGTTTCCCTTCTGCAGTGTCAGTTAGATGCATGGAATAGACAAAAGGATATTGAACGCATTGGTTTCCACTCTGTAGAAGTCGATGGTGACGATCCAGATGATGATACTTGTGGCATCTGTGGGGATGGCGGGGATTCGATCTGTTGCGATGGCTGTCCATCAACATTTCATCAGAGCTGCTTGAATATACAGATGCTTCCTCCTGGTGATTGGCACTGTCCAAATTGTACATGCAAGTTTTGTGGGGTAGCAAATGAGAATGTTGTGGAAGAGGATGATATAACTGTTGATGCACTACTCACTTGCAGCCTCTGTGGGAAAAAATGTCACACATCATGCTGTCAGGAGATGGATGGTAGTCCTGCTGCTTCTCCTAGTTTAGACTCTTCCTTCTGTGGGCAGAAATGCAGAGAGCTCTTTGAGAATTTGAAGAAGTATCTTGGGGTTAAACATGAACTAGAATCAGGATTTTCATGGACACTCGTTCATAGAACTGATGAAGATGAAGGGTTCCCTCAAAGGATCGAATGCAATTCTAAGCTAGCTGTTGCTTTGACTGTTATGGATGAATGCTTTTTGCCTATTGTTGACAGGAGGAGCGGCATCAATTTAATTCATAATGTTCTTTATAATTGTGGATCAAACTTCAATCGACTGAACTACAGTGGCTTCTACACCGCTATTTTAGAGAGAGGCGATGAAATAATTTCTGCTGCATCAATCAGGTTTCATGGTACAAAGTTAGCAGAGATGCCATACATCGGAACTCGCCACATATATAGGCGACAAGGGATGTGCCGTCGGCTTTTTTGTGCCATTGAATCGGCTCTATGCTCTCTCAAGGTTGAGAAATTGGTTATCCCTGCAATTGCTGAACTCATGCATACATGGACAGAAGTTTTTGGTTTTATTCCTGTTGAGGAATCATTCAAGCAAGAAATGAGGTCAATGAATATGCTTGTATTCCCTGGAATAGACATGCTACAGAAGTTGCTAGTGGATCAAGAAAATGAGGGGAACGGGACAGCAGACCCAGGCATAAAGCAATTAGAATGCAAAGGCAAGGATTTAATAAAGCATGGGAGCGGAAGCAAATCAGATAGTGGTTCACCTGCTACTATTGATCATCATGGATCTGATGAAACCAGTTTGCCTCATATTAATGAAACAGTTGATGAAGCTGCTGCTATGGTTTCTGGTTCTCAATGTCTCGGTGTCTCCTTCAATGATAGTCCTGTGATGAGTGGTTCTTTAGATGGTTCTGATGAACATAAAAATTTAGATTCCACTGAGAGGAGTGTATCTCCTGACTCTCTTTTGGGGGCCGAATTACCTCGATCTACTTTGGACAAGGAATACCCTGTTGATACAAGCCACAAGGCTGTTGCAACAGAAGATAAGCCAGTGCTGGATTCTCCTGGTGAAGATAAAATTCAGTCTACCTCTCAGGGTCCTGGTGAGGCTCCAGCGATAGAAGATGAGCCAGTGGTGGATTCTCATGTTAAAGATAAAATGCAGTCTACCTGCCAGGGTCCTGGTGAGGCTTCAGTGATAGAAGATGAGCCAGCGCTGGATTCTCCTGTTGAAAATAAAATGCAGTCTGCCTCTCAGGGTCCTGGTGAGGCTCTAGCAACAGAAGATGAGCAGGTGCTGGATTCTCCTGATGAAGATAAAATGCAGTCTATCTCTCAGGGAACTGGTGAGGCTCTAGCGACAGAAGATGAGCCAGTGCTGGATTCTCCTCTTGAAGATAAAATGCAGTCTACCTCTCAGGGTCCTGGTGAGGTTCTAGCGGCAGAAGATGAGCCAGTGCTGGATTCTCCTGTTGAAGATAAAATGCAGTTTACCTCTCAAGATGCTGCTGTTTCTTTGAATGACACTTCCATGATGAGTAGTAGGTCGTCGGATAATTCGAATGAACATAACATCCAGGTTTCAACCAAGGGGGCCACATCTTCGGACTCCAATCCAGGTACCAGGTCAGTGGAGTATGCTTCTGATGTCAACTACCAGTCTTGCCCTTACATAAGCCATGATGAAAAGGTGGAAATAGAATCAGTTTCAGATTCTTCTCTGAAAGAGAATTCTTCAAAATCTTTGGAAGAAAGTGCTTTGGATGATTCCCATGAAGAGAATGTTGATGCTTCCTGTCCTCAGCCTATTCCTTCTTCAGGAGAAACTTTCGCCAAGAACCATGCTGAGGAGGTTAACGGAAATCAAGATTCCAGTTTCTGTGGTGCTAGTGAGAGTTCCTTGCTCAACAATTGTGATTTAAATATTCAACTTGATAGCAAGGCAAAAGACGAATCATATGTGGCTTCAGAAGTCGCGTCTGATGCAATGCAGTGTGAAAAGAGCATTCCTCATGCATCTAGTGATGGTTCCAAGGCTGATTCTGGGAAAGCTGAATCTGTTTCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005700 GO:0006323 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006996 GO:0007063 GO:0007276 GO:0007283 GO:0008150 GO:0008270 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010639 GO:0010948 GO:0016043 GO:0016462 GO:0016581 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019219 GO:0019222 GO:0019953 GO:0022414 GO:0030261 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031491 GO:0031974 GO:0031981 GO:0032501 GO:0032504 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0034728 GO:0035327 GO:0042623 GO:0042766 GO:0043044 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044703 GO:0044877 GO:0045786 GO:0045875 GO:0045892 GO:0045934 GO:0046872 GO:0046914 GO:0048232 GO:0048519 GO:0048523 GO:0048609 GO:0050789 GO:0050794 GO:0051098 GO:0051101 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051312 GO:0051704 GO:0051726 GO:0051983 GO:0051985 GO:0060255 GO:0060341 GO:0065007 GO:0065009 GO:0070013 GO:0070603 GO:0070615 GO:0071103 GO:0071824 GO:0071840 GO:0071922 GO:0071923 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:1901363 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1903827 GO:1903828 GO:1904949 GO:1905634 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas PHD

Protein Analysis

1465

Amino Acids

160.89

Weight (kDa)

5.5

Isoelectric Point (pI)

57.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2601
AarI CACCTGC 2 cut(s) 3091, 3607
Acc36I ACCTGC 5 cut(s) 1163, 1232, 3091, 3503, 3607
Acc65I GGTACC 1 cut(s) 3944
AccB1I GGYRCC 1 cut(s) 3944
AccB7I CCANNNNNTGG 2 cut(s) 509, 1742
AccBSI CCGCTC 2 cut(s) 2571, 3057
AccI GTMKAC 4 cut(s) 544, 3410, 3491, 3734
AccII CGCG 1 cut(s) 4301
AciI CCGC 7 cut(s) 304, 758, 2100, 2571, 2649, 3057, 3764
AclWI GGATC 8 cut(s) 311, 324, 1633, 2057, 2501, 2617, 2979, 3112
AcoI YGGCCR 1 cut(s) 1572
AcsI RAATTY 9 cut(s) 52, 404, 568, 775, 1907, 2395, 3247, 3402, 4054
AcuI CTGAAG 6 cut(s) 42, 810, 1605, 3501, 4125, 4275
AdeI CACNNNGTG 1 cut(s) 509
AfaI GTAC 8 cut(s) 575, 921, 1229, 1246, 1678, 2200, 2707, 3946
AfeI AGCGCT 1 cut(s) 3540
AfiI CCNNNNNNNGG 9 cut(s) 34, 509, 1622, 1742, 2367, 2732, 3264, 4142, 4171
AflIII ACRYGT 1 cut(s) 547
AloI GAACNNNNNNTCC 2 cut(s) 3882, 3914
Alw21I GWGCWC 3 cut(s) 124, 652, 2388
Alw26I GTCTC 4 cut(s) 663, 1203, 3185, 3193
AlwI GGATC 8 cut(s) 311, 324, 1633, 2057, 2501, 2617, 2979, 3112
AlwNI CAGNNNCTG 3 cut(s) 2283, 3622, 4203
Aor51HI AGCGCT 1 cut(s) 3540
AoxI GGCC 7 cut(s) 25, 764, 896, 1193, 1572, 3294, 3918
ApeKI GCWGC 8 cut(s) 1162, 2148, 2277, 2336, 2684, 3152, 3155, 3830
ApoI RAATTY 9 cut(s) 52, 404, 568, 775, 1907, 2395, 3247, 3402, 4054
AseI ATTAAT 1 cut(s) 3132
Asp700I GAANNNNTTC 2 cut(s) 443, 481
Asp718I GGTACC 1 cut(s) 3944
AspLEI GCGC 4 cut(s) 104, 1282, 1938, 3541
AsuII TTCGAA 1 cut(s) 3884
AsuNHI GCTAGC 1 cut(s) 2512
AvaII GGWCC 9 cut(s) 110, 224, 998, 1058, 1257, 3422, 3503, 3584, 3746
BalI TGGCCA 1 cut(s) 1574
BamHI GGATCC 1 cut(s) 316
BanI GGYRCC 1 cut(s) 3944
BanII GRGCYC 2 cut(s) 652, 2388
BarI GAAGNNNNNNTAC 2 cut(s) 557, 589
BbsI GAAGAC 1 cut(s) 1611
Bbv12I GWGCWC 3 cut(s) 124, 652, 2388
BbvCI CCTCAGC 2 cut(s) 4125, 4170
BbvI GCAGC 8 cut(s) 1149, 2135, 2289, 2323, 2671, 3139, 3142, 3817
BceAI ACGGC 3 cut(s) 919, 1600, 2754
BcgI CGANNNNNNTGC 2 cut(s) 481, 515
BclI TGATCA 2 cut(s) 1480, 3094
BcoDI GTCTC 4 cut(s) 663, 1203, 3185, 3193
BfmI CTRYAG 7 cut(s) 261, 707, 1882, 1982, 2043, 2632, 2954
BfoI RGCGCY 2 cut(s) 105, 3542
BfuAI ACCTGC 5 cut(s) 1163, 1232, 3091, 3503, 3607
BglII AGATCT 1 cut(s) 17
BlpI GCTNAGC 1 cut(s) 651
Bme18I GGWCC 9 cut(s) 110, 224, 998, 1058, 1257, 3422, 3503, 3584, 3746
BmrI ACTGGG 1 cut(s) 1752
BmtI GCTAGC 1 cut(s) 2516
BmuI ACTGGG 1 cut(s) 1752
BpiI GAAGAC 1 cut(s) 1611
BplI GAGNNNNNCTC 4 cut(s) 3259, 3291, 4318, 4350
BpmI CTGGAG 3 cut(s) 791, 1862, 3420
Bpu10I CCTNAGC 2 cut(s) 4125, 4170
Bpu1102I GCTNAGC 1 cut(s) 651
Bpu14I TTCGAA 1 cut(s) 3884
BpuEI CTTGAG 2 cut(s) 2795, 3807
BsaAI YACGTR 1 cut(s) 1142
BsaJI CCNNGG 7 cut(s) 601, 1025, 2938, 3004, 3499, 3912, 4356
BsaWI WCCGGW 2 cut(s) 85, 319
Bsc4I CCNNNNNNNGG 9 cut(s) 34, 509, 1622, 1742, 2367, 2732, 3264, 4142, 4171
Bse3DI GCAATG 4 cut(s) 253, 273, 929, 4317
BseDI CCNNGG 7 cut(s) 601, 1025, 2938, 3004, 3499, 3912, 4356
BseLI CCNNNNNNNGG 9 cut(s) 34, 509, 1622, 1742, 2367, 2732, 3264, 4142, 4171
BseMI GCAATG 4 cut(s) 253, 273, 929, 4317
BseXI GCAGC 8 cut(s) 1149, 2135, 2289, 2323, 2671, 3139, 3142, 3817
BseYI CCCAGC 1 cut(s) 1237
BsgI GTGCAG 3 cut(s) 1191, 1808, 1826
Bsh1236I CGCG 1 cut(s) 4301
BshFI GGCC 7 cut(s) 27, 766, 898, 1195, 1574, 3296, 3920
BshNI GGYRCC 1 cut(s) 3944
BsiHKAI GWGCWC 3 cut(s) 124, 652, 2388
BsiSI CCGG 2 cut(s) 86, 320
BslFI GGGAC 2 cut(s) 237, 3007
BslI CCNNNNNNNGG 9 cut(s) 34, 509, 1622, 1742, 2367, 2732, 3264, 4142, 4171
BsmAI GTCTC 4 cut(s) 663, 1203, 3185, 3193
BsmFI GGGAC 2 cut(s) 237, 3007
BsmI GAATGC 7 cut(s) 530, 760, 1866, 2504, 2546, 3029, 4329
BsnI GGCC 7 cut(s) 27, 766, 898, 1195, 1574, 3296, 3920
Bsp119I TTCGAA 1 cut(s) 3884
Bsp1286I GDGCHC 3 cut(s) 124, 652, 2388
Bsp1407I TGTACA 2 cut(s) 1227, 2198
Bsp1720I GCTNAGC 1 cut(s) 651
BspACI CCGC 7 cut(s) 304, 758, 2100, 2571, 2649, 3057, 3764
BspANI GGCC 7 cut(s) 27, 766, 898, 1195, 1574, 3296, 3920
BspFNI CGCG 1 cut(s) 4301
BspMAI CTGCAG 1 cut(s) 1986
BspMI ACCTGC 5 cut(s) 1163, 1232, 3091, 3503, 3607
BspOI GCTAGC 1 cut(s) 2516
BspPI GGATC 8 cut(s) 311, 324, 1633, 2057, 2501, 2617, 2979, 3112
BspT104I TTCGAA 1 cut(s) 3884
BspT107I GGYRCC 1 cut(s) 3944
BsrBI CCGCTC 2 cut(s) 2571, 3057
BsrDI GCAATG 4 cut(s) 253, 273, 929, 4317
BsrGI TGTACA 2 cut(s) 1227, 2198
BssECI CCNNGG 7 cut(s) 601, 1025, 2938, 3004, 3499, 3912, 4356
BssT1I CCWWGG 2 cut(s) 3912, 4356
Bst4CI ACNGT 9 cut(s) 444, 947, 1786, 1874, 2189, 2257, 2530, 2636, 3142
Bst6I CTCTTC 5 cut(s) 146, 1025, 2235, 2362, 4095
BstAUI TGTACA 2 cut(s) 1227, 2198
BstBAI YACGTR 1 cut(s) 1142
BstBI TTCGAA 1 cut(s) 3884
BstC8I GCNNGC 4 cut(s) 344, 1940, 2514, 3537
BstENI CCTNNNNNAGG 1 cut(s) 4140
BstFNI CGCG 1 cut(s) 4301
BstH2I RGCGCY 2 cut(s) 105, 3542
BstHHI GCGC 4 cut(s) 104, 1282, 1938, 3541
BstMAI GTCTC 4 cut(s) 663, 1203, 3185, 3193
BstMWI GCNNNNNNNGC 6 cut(s) 1247, 1277, 1288, 1765, 1935, 4307
BstNSI RCATGY 4 cut(s) 346, 551, 2205, 2953
BstSFI CTRYAG 7 cut(s) 261, 707, 1882, 1982, 2043, 2632, 2954
BstUI CGCG 1 cut(s) 4301
BstV1I GCAGC 8 cut(s) 1149, 2135, 2289, 2323, 2671, 3139, 3142, 3817
BstV2I GAAGAC 1 cut(s) 1611
BstX2I RGATCY 3 cut(s) 17, 316, 3104
BstXI CCANNNNNNTGG 2 cut(s) 3506, 3955
BstYI RGATCY 3 cut(s) 17, 316, 3104
BsuRI GGCC 7 cut(s) 27, 766, 898, 1195, 1574, 3296, 3920
BtgZI GCGATG 2 cut(s) 2133, 2682
BtsI GCAGTG 2 cut(s) 1991, 4322
BveI ACCTGC 5 cut(s) 1163, 1232, 3091, 3503, 3607
Cac8I GCNNGC 4 cut(s) 344, 1940, 2514, 3537
CaiI CAGNNNCTG 3 cut(s) 2283, 3622, 4203
CfoI GCGC 4 cut(s) 104, 1282, 1938, 3541
CseI GACGC 1 cut(s) 4290
CsiI ACCWGGT 1 cut(s) 3947
Csp6I GTAC 8 cut(s) 574, 920, 1228, 1245, 1677, 2199, 2706, 3945
CspCI CAANNNNNGTGG 2 cut(s) 2194, 2229
CviQI GTAC 8 cut(s) 574, 920, 1228, 1245, 1677, 2199, 2706, 3945
DraI TTTAAA 1 cut(s) 4245
DraIII CACNNNGTG 1 cut(s) 509
EaeI YGGCCR 1 cut(s) 1572
Eam1104I CTCTTC 5 cut(s) 146, 1025, 2235, 2362, 4095
EarI CTCTTC 5 cut(s) 146, 1025, 2235, 2362, 4095
Ecl136II GAGCTC 2 cut(s) 650, 2386
Eco130I CCWWGG 2 cut(s) 3912, 4356
Eco147I AGGCCT 1 cut(s) 1195
Eco24I GRGCYC 2 cut(s) 652, 2388
Eco47I GGWCC 9 cut(s) 110, 224, 998, 1058, 1257, 3422, 3503, 3584, 3746
Eco47III AGCGCT 1 cut(s) 3540
Eco53kI GAGCTC 2 cut(s) 650, 2386
Eco57I CTGAAG 6 cut(s) 42, 810, 1605, 3501, 4125, 4275
EcoICRI GAGCTC 2 cut(s) 650, 2386
EcoNI CCTNNNNNAGG 1 cut(s) 4140
EcoO109I RGGNCCY 5 cut(s) 224, 3422, 3503, 3584, 3746
EcoRI GAATTC 1 cut(s) 4054
EcoT14I CCWWGG 2 cut(s) 3912, 4356
EcoT22I ATGCAT 3 cut(s) 2002, 2856, 4342
EcoT38I GRGCYC 2 cut(s) 652, 2388
ErhI CCWWGG 2 cut(s) 3912, 4356
FalI AAGNNNNNCTT 2 cut(s) 1663, 1695
FaqI GGGAC 2 cut(s) 237, 3007
FauI CCCGC 3 cut(s) 297, 751, 2093
FauNDI CATATG 2 cut(s) 1638, 4282
FbaI TGATCA 2 cut(s) 1480, 3094
FblI GTMKAC 4 cut(s) 544, 3410, 3491, 3734
FriOI GRGCYC 2 cut(s) 652, 2388
GlaI GCGC 4 cut(s) 103, 1281, 1937, 3540
GsaI CCCAGC 1 cut(s) 1241
GsuI CTGGAG 3 cut(s) 791, 1862, 3420
HaeII RGCGCY 2 cut(s) 105, 3542
HaeIII GGCC 7 cut(s) 27, 766, 898, 1195, 1574, 3296, 3920
HapII CCGG 2 cut(s) 86, 320
HgaI GACGC 1 cut(s) 4290
HhaI GCGC 4 cut(s) 104, 1282, 1938, 3541
Hin6I GCGC 4 cut(s) 102, 1280, 1936, 3539
HinP1I GCGC 4 cut(s) 102, 1280, 1936, 3539
HincII GTYRAC 6 cut(s) 556, 1711, 1774, 2560, 3976, 4180
HindII GTYRAC 6 cut(s) 556, 1711, 1774, 2560, 3976, 4180
HindIII AAGCTT 2 cut(s) 866, 1664
HpaI GTTAAC 2 cut(s) 1711, 4180
HpaII CCGG 2 cut(s) 86, 320
Hpy99I CGWCG 2 cut(s) 2775, 3877
HpyCH4III ACNGT 9 cut(s) 444, 947, 1786, 1874, 2189, 2257, 2530, 2636, 3142
HpyCH4IV ACGT 3 cut(s) 1111, 1141, 1550
HpyF10VI GCNNNNNNNGC 6 cut(s) 1247, 1277, 1288, 1765, 1935, 4307
HpySE526I ACGT 3 cut(s) 1111, 1141, 1550
HspAI GCGC 4 cut(s) 102, 1280, 1936, 3539
KpnI GGTACC 1 cut(s) 3948
Ksp22I TGATCA 2 cut(s) 1480, 3094
KspAI GTTAAC 2 cut(s) 1711, 4180
LmnI GCTCC 8 cut(s) 208, 488, 647, 959, 1488, 2568, 3054, 3439
Lsp1109I GCAGC 8 cut(s) 1149, 2135, 2289, 2323, 2671, 3139, 3142, 3817
MabI ACCWGGT 1 cut(s) 3947
MaeII ACGT 3 cut(s) 1111, 1141, 1550
MaeIII GTNAC 3 cut(s) 356, 2057, 2297
MbiI CCGCTC 2 cut(s) 2571, 3057
MfeI CAATTG 2 cut(s) 2838, 4234
MflI RGATCY 3 cut(s) 17, 316, 3104
MhlI GDGCHC 3 cut(s) 124, 652, 2388
MlsI TGGCCA 1 cut(s) 1574
MluNI TGGCCA 1 cut(s) 1574
MlyI GAGTC 7 cut(s) 1069, 1455, 1769, 1958, 2348, 3275, 3926
MmeI TCCRAC 5 cut(s) 372, 1106, 1157, 1275, 3855
Mox20I TGGCCA 1 cut(s) 1574
Mph1103I ATGCAT 3 cut(s) 2002, 2856, 4342
MroXI GAANNNNTTC 2 cut(s) 443, 481
MscI TGGCCA 1 cut(s) 1574
MslI CAYNNNNRTG 4 cut(s) 915, 1596, 3132, 3854
Msp20I TGGCCA 1 cut(s) 1574
MspI CCGG 2 cut(s) 86, 320
MunI CAATTG 2 cut(s) 2838, 4234
Mva1269I GAATGC 7 cut(s) 530, 760, 1866, 2504, 2546, 3029, 4329
MvnI CGCG 1 cut(s) 4301
MwoI GCNNNNNNNGC 6 cut(s) 1247, 1277, 1288, 1765, 1935, 4307
NdeI CATATG 2 cut(s) 1638, 4282
NheI GCTAGC 1 cut(s) 2512
NmuCI GTSAC 2 cut(s) 2057, 2297
NsiI ATGCAT 3 cut(s) 2002, 2856, 4342
NspI RCATGY 4 cut(s) 346, 551, 2205, 2953
NspV TTCGAA 1 cut(s) 3884
PaeI GCATGC 1 cut(s) 346
PaqCI CACCTGC 2 cut(s) 3091, 3607
PceI AGGCCT 1 cut(s) 1195
PciI ACATGT 1 cut(s) 547
PcsI WCGNNNNNNNCGW 2 cut(s) 2058, 3881
PctI GAATGC 7 cut(s) 530, 760, 1866, 2504, 2546, 3029, 4329
PdmI GAANNNNTTC 2 cut(s) 443, 481
PflMI CCANNNNNTGG 2 cut(s) 509, 1742
PleI GAGTC 7 cut(s) 1069, 1454, 1769, 1958, 2348, 3275, 3926
PpsI GAGTC 7 cut(s) 1069, 1454, 1769, 1958, 2348, 3275, 3926
Ppu21I YACGTR 1 cut(s) 1142
PpuMI RGGWCCY 5 cut(s) 224, 3422, 3503, 3584, 3746
PscI ACATGT 1 cut(s) 547
PshBI ATTAAT 1 cut(s) 3132
PsiI TTATAA 1 cut(s) 2601
Psp124BI GAGCTC 2 cut(s) 652, 2388
Psp5II RGGWCCY 5 cut(s) 224, 3422, 3503, 3584, 3746
PspFI CCCAGC 1 cut(s) 1237
PspPPI RGGWCCY 5 cut(s) 224, 3422, 3503, 3584, 3746
PstI CTGCAG 1 cut(s) 1986
PstNI CAGNNNCTG 3 cut(s) 2283, 3622, 4203
PsuI RGATCY 3 cut(s) 17, 316, 3104
RsaI GTAC 8 cut(s) 575, 921, 1229, 1246, 1678, 2200, 2707, 3946
RsaNI GTAC 8 cut(s) 574, 920, 1228, 1245, 1677, 2199, 2706, 3945
RseI CAYNNNNRTG 4 cut(s) 915, 1596, 3132, 3854
SacI GAGCTC 2 cut(s) 652, 2388
SchI GAGTC 7 cut(s) 1069, 1455, 1769, 1958, 2348, 3275, 3926
SduI GDGCHC 3 cut(s) 124, 652, 2388
SexAI ACCWGGT 1 cut(s) 3947
SfcI CTRYAG 7 cut(s) 261, 707, 1882, 1982, 2043, 2632, 2954
SfuI TTCGAA 1 cut(s) 3884
SinI GGWCC 9 cut(s) 110, 224, 998, 1058, 1257, 3422, 3503, 3584, 3746
SmiI ATTTAAAT 1 cut(s) 4245
SmiMI CAYNNNNRTG 4 cut(s) 915, 1596, 3132, 3854
SmlI CTYRAG 2 cut(s) 2810, 3822
SmoI CTYRAG 2 cut(s) 2810, 3822
SphI GCATGC 1 cut(s) 346
SseBI AGGCCT 1 cut(s) 1195
SsiI CCGC 7 cut(s) 304, 758, 2100, 2571, 2649, 3057, 3764
SspI AATATT 1 cut(s) 4249
SstI GAGCTC 2 cut(s) 652, 2388
StuI AGGCCT 1 cut(s) 1195
StyI CCWWGG 2 cut(s) 3912, 4356
SwaI ATTTAAAT 1 cut(s) 4245
TaaI ACNGT 9 cut(s) 444, 947, 1786, 1874, 2189, 2257, 2530, 2636, 3142
TaiI ACGT 3 cut(s) 1114, 1144, 1553
TatI WGTACW 4 cut(s) 573, 1227, 1676, 2198
TauI GCSGC 2 cut(s) 2574, 3767
TseFI GTSAC 2 cut(s) 2057, 2297
TseI GCWGC 8 cut(s) 1162, 2148, 2277, 2336, 2684, 3152, 3155, 3830
Tsp45I GTSAC 2 cut(s) 2057, 2297
TspGWI ACGGA 4 cut(s) 1139, 1439, 1765, 4197
Van91I CCANNNNNTGG 2 cut(s) 509, 1742
VpaK11BI GGWCC 9 cut(s) 110, 224, 998, 1058, 1257, 3422, 3503, 3584, 3746
VspI ATTAAT 1 cut(s) 3132
XagI CCTNNNNNAGG 1 cut(s) 4140
XapI RAATTY 9 cut(s) 52, 404, 568, 775, 1907, 2395, 3247, 3402, 4054
XbaI TCTAGA 1 cut(s) 1216
XceI RCATGY 4 cut(s) 346, 551, 2205, 2953
XmiI GTMKAC 4 cut(s) 544, 3410, 3491, 3734
XmnI GAANNNNTTC 2 cut(s) 443, 481
Zsp2I ATGCAT 3 cut(s) 2002, 2856, 4342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.