pycom13g17450

Ethylene-responsive protein kinase Le-CTR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007173
Physical Location & Seq
Forward (+)
13204666 .. 13211633
6968 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3363 bp
ATGCAAATCCCCAATTCTGACGAAGCTGCCCCCGAAAACCCTAAGGAACCAGAGCAATCAGAAGCTGAAGCCTCCAATCCGACTGTCGAAGAGGAAGATGGCGGAGCCGCTGCCGACGGCGATGACGGCGACTCGAGGGTTGTCGATATTTCCGGCAAAAGCTTACACTTTTCGATCAGGGAGAATTCTGAGGATGCCGGGGCTTTGTATGTGTATAAAAACGTGTTTAATTTATTGCCGAAGTCGATTGCCTCTCACAAGCGGTTGAGAACGCTGAAGTTCTTTGGGAATGGGATTAATTTATTTTCGCCGTCCGCGGCGATGGAGTTTGAGAGCTTGGAATGCTTGCAAGTGAGGATGTCGTCGCCGGAGTTTGGTGGGTTGCCGTTGGATAAATTGAGTGGCTTGAAGGAGCTTGAGCTTTCCAAAGTGCCGCCGAGGCCTTCGGGGTTTCAGATATTGAGCGAGATTGCTCGGCTCAAGTGCCTCACCAAGCTCTCTGTTTGTCACTTCTCTATAAGATACCTCCCTCCAGAAATTGGCTGCTTAAACACTTTGGAATATCTAGATCTTTCATTCAACAAGATAAAAAGTTTGCCTGCTGAGATCAGTAATTTAAATGCCTTGATATCATTAAAAGTTGCTAATAATAAATTGGTGGAATTGCCTTCGACTTTGTCCTCTTTGCAGAGGTTGGAGACAATGGACCTGTCCAATAATAGATTGACATCACTAGGATACCTTGAACTTGACTTGATGCACAACCTCCAGATTTTAAATCTTCAGTACAACAAGCTTCTCACTTTCTGTCAAATACCCTCATGGATATGCTGCAATTTAGAAGGAACTGGGAAGGACACACTGGGTGATGATTCTACCAGTTCCTCTGTAGAAATGGAAGTATATGAAGCCCCCCTTGGGAACAATAATGAAAGTTTATTTCGCAGAGGCTTTCATCATAGCTCAGCAAGTTTAACTTTCGCACATTCATCGAACAGTAGGTGTTGTGCAACTCGGAGGTCAGGTAGGTGGAGGAAGCAAGGGTACAGTTTGCAGCAAAGAGCTCGTCAAGAACGCTTAAACAACAGTAGAAAGTTGAAAGGTCTTGATCCTTCCAAGCGGTTACACGTGCGGGAAGATGAAGAAAGCAAACCAGGCATCCAAGATCTTCTTGCTTCTGAATCTTATCCTGAAGGTGCATCAGACATTATCAATCTGGATGATGATGGCGATCAAGATGTACTATCTAGGGAAGTTCAAAGTGAAAATATTGTGTGCCATGATGTGAGCTTGAAGGAGCGTTTTGTAGGAAATTGCTCATCTGTGACTCCAGACTCTAGCACACTAGATGAAAGTGATGAAAAGGATTGTTGTGAGTTTGATGCATCGTCAGCCTCCAATCAACAGGTTTATGGTGAGGAAGATGAAGGTTCATCTTCAGATGCATCAAAAAGTATTTGTCAACCCAAAAGGCACCCTGATGGGAACCTTGATAATCCGAGACGGCCCAAACATCCTAGATACTGTCCTGATGGTTCAAACTTGTCTCGGAAATATAATGACTTCTCGGTTTGCAGCACTGAGGACCATCTTTCTGATGGCTTTTATGATGCAGGGCGTGACAGGCCATTCATGCGACTAGAACTTTATCAGGAAAAATTCCACCTTGACTCACGTGAAGTCATTCTTGTGGACAGGCAATGGGATAAAGAGTTGGATGTTATTTTACGTTCTGCCCAAGAATTGGTGAATCGTTTAAATACAGATGGAAATAAGGCTGATATGCTGCAGATTGCATCATTGCTTGCTCTGTTTGTATCAGATCACTTTGGGGGAAGTGATAGGAGTGCTCTTGTTGAATGGGCACGAAAAGCTAATCCTCTTTCAGACTACAGGAAGCCTTTTGTTTGCACCTGCCCAACTGGAAACAAGGGCTTTATTAGTTTGTCCACTAAACCAGTTTTAAAGAATTTTGAAGATATCGTTTTTTCTGATCTCTGTGAGAAATCCCTTCGTTCTATTAAAGCACGACGAAAATCCATTGTGGTTCCCATTGGAACCTTGCAGTTTGGTGTGTGTCGGCATAGAGCATTGCTATTGAAGTATCTCTGTGATTGGGTGGAGCCTCGGGTACCTTGTGAGCTTGTTAGGGGTTACATGGATTTCATGCCACATGCGTGGAATATTGTATCAATCAAGAGAGGTGCCTCAGAGATTCGAATGGTAGTGGATGCATGTCGTCCGCGTGATTTAAGAGTGGAGACTGATCCTGAATATTATTGCAGGTATATTCCTCTCAGCCGGACCAAAGTTCATCTTCCAATTCGAAGCAGCCCTGCTCCTACCTCCTTCCCTTCTATGCCCTCTTGTGATGAAACTCTAAAAAACTCCGTTACTTCTCTCGTCCGGTGCAAATATGGATCAAATGAGGCAGCAGGAAAGATGCGTACTTCCATGGTATATGGAAATTCAACGGATGAAATTAGGAACTTTGACTACAACTGTTTAGGGGAAATAAGGATTTTAGGTGCTTTGAAACACCCTTGCATAGTGGAAATGTATGGACATCAGATATCTTCCAAGTGGGTTCCTTCTGGGAATGGTAGTCCTGAGCATCGTATATTGCAGTCTATAATTTGGATGGAGGACATAAAAGGAGGTTCCTTACAGAATTATATGGAAAAGCTATCAAAAGCTGGTGACAAGCGTCTTCCAGTGGAGTTAGCTTTGTGCATCGCTAAAAATGTTGCATGTGCATTGGTGGAGCTGCATTCAAAGCACATTATTCACCGGGACGTAAAAAGTGCAAACATTTTGATTGATCTGGATAGGAAGAGAGCTGATGGAACACATGTTGTGAAGCTCTGTGATTTTGATAGGGCAGTTCCCCTTCGATCTTACTTGCATACATGCTGCATCGCCCACATAGGAATACCTCCTGCTAATGTTTGTGCTGGAACACCTCGCTGGATGGCCCCAGAGGTTTTGCGAGCAGTGCATGAACGAAATATATATGGATTGGAAATTGATATCTGGTCGTTTGGGTGCTTGCTGTTGGAAATGTTGACTCTGCAAATTCCGTACTTCGGAGTACCTGAATTGGAGATTCATGAACTTCTGACGAGGGGAAAACGACCAAGGTTAACAGATGACTTGGAGGCGTTTCGGTCATTGAATGAGCCGGTAATGACTCAAGCAGGCGCGGAACTTGAGGGAACAGAGGCTGACTTAGACACACTGAGATTTCTTGTTGACTTGTTCTACCAATGTACGGAGGAGAATCCGGAGAATCGTCCGACGGCTGACAACCTCTACGAATTGATACTGGAACACAGCAAAACTCTTACAAACTCGGAAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 LRR_8 Pkinase

Protein Analysis

1121

Amino Acids

125.83

Weight (kDa)

5.64

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 154 - 264 1.7e-11 Leucine-rich repeat region
EDR1_CTR1_ARMC3_pept PF14381 668 - 744 5.1e-12 EDR1/CTR1/ARMC3, peptidase-like
Pkinase PF00069 832 - 1105 1.9e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 834 - 1048 6.1e-23 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1922
Acc36I ACCTGC 2 cut(s) 1922, 2274
Acc65I GGTACC 1 cut(s) 2131
AccB1I GGYRCC 3 cut(s) 1473, 2131, 2204
AccB7I CCANNNNNTGG 2 cut(s) 539, 1744
AccII CGCG 3 cut(s) 317, 2245, 3203
AccIII TCCGGA 1 cut(s) 3283
AclWI GGATC 3 cut(s) 1103, 2261, 2428
AcsI RAATTY 5 cut(s) 184, 1658, 1969, 2467, 3075
AcuI CTGAAG 5 cut(s) 87, 296, 767, 1212, 1422
AcvI CACGTG 2 cut(s) 1129, 1676
AdeI CACNNNGTG 1 cut(s) 866
AfaI GTAC 8 cut(s) 788, 1046, 1242, 2133, 2449, 3083, 3093, 3271
AfiI CCNNNNNNNGG 6 cut(s) 374, 539, 918, 1744, 3086, 3271
AflIII ACRYGT 3 cut(s) 222, 1126, 2851
AjnI CCWGG 1 cut(s) 1153
AjuI GAANNNNNNNTTGG 2 cut(s) 900, 932
AleI CACNNNNGTG 1 cut(s) 2176
Alw21I GWGCWC 2 cut(s) 1066, 1852
Alw26I GTCTC 4 cut(s) 692, 1495, 1551, 2255
AlwI GGATC 3 cut(s) 1103, 2261, 2428
AlwNI CAGNNNCTG 2 cut(s) 65, 3224
Ama87I CYCGRG 2 cut(s) 133, 2127
Aor13HI TCCGGA 1 cut(s) 3283
AoxI GGCC 4 cut(s) 440, 1505, 1625, 2973
ApoI RAATTY 5 cut(s) 184, 1658, 1969, 2467, 3075
ArsI GACNNNNNNTTYG 2 cut(s) 1051, 1083
AseI ATTAAT 1 cut(s) 297
Asp700I GAANNNNTTC 1 cut(s) 1683
Asp718I GGTACC 1 cut(s) 2131
AspLEI GCGC 1 cut(s) 3203
AspS9I GGNCC 5 cut(s) 706, 1506, 1585, 2304, 2974
AsuC2I CCSGG 2 cut(s) 199, 2792
AsuHPI GGTGA 6 cut(s) 481, 878, 1427, 1759, 2711, 2780
AsuII TTCGAA 2 cut(s) 2218, 2326
AvaI CYCGRG 2 cut(s) 133, 2127
AvaII GGWCC 3 cut(s) 706, 1585, 2304
AxyI CCTNAGG 1 cut(s) 42
BaeGI GKGCMC 1 cut(s) 1867
BanI GGYRCC 3 cut(s) 1473, 2131, 2204
BanII GRGCYC 1 cut(s) 1066
BarI GAAGNNNNNNTAC 2 cut(s) 1028, 1060
BbrPI CACGTG 2 cut(s) 1129, 1676
BbsI GAAGAC 1 cut(s) 2702
Bbv12I GWGCWC 2 cut(s) 1066, 1852
BceAI ACGGC 6 cut(s) 133, 142, 295, 370, 1520, 3315
BcgI CGANNNNNNTGC 4 cut(s) 972, 1006, 1046, 1080
BciT130I CCWGG 1 cut(s) 1155
BciVI GTATCC 1 cut(s) 731
BcnI CCSGG 2 cut(s) 199, 2792
BcoDI GTCTC 4 cut(s) 692, 1495, 1551, 2255
BfaI CTAG 7 cut(s) 566, 734, 1248, 1338, 1346, 1518, 1640
BfmI CTRYAG 3 cut(s) 888, 1787, 1891
BfuAI ACCTGC 2 cut(s) 1922, 2274
BfuI GTATCC 1 cut(s) 731
BglI GCCNNNNNGGC 1 cut(s) 439
BglII AGATCT 2 cut(s) 568, 1165
BlpI GCTNAGC 1 cut(s) 964
Bme1390I CCNGG 3 cut(s) 199, 1155, 2792
Bme18I GGWCC 3 cut(s) 706, 1585, 2304
BmeT110I CYCGRG 2 cut(s) 133, 2127
BmgT120I GGNCC 5 cut(s) 706, 1506, 1585, 2304, 2974
BmrFI CCNGG 3 cut(s) 199, 1155, 2792
BmrI ACTGGG 2 cut(s) 858, 872
BmuI ACTGGG 2 cut(s) 858, 872
BoxI GACNNNNGTC 1 cut(s) 2706
BpiI GAAGAC 1 cut(s) 2702
BpmI CTGGAG 3 cut(s) 516, 752, 1314
Bpu10I CCTNAGC 1 cut(s) 2610
Bpu1102I GCTNAGC 1 cut(s) 964
Bpu14I TTCGAA 2 cut(s) 2218, 2326
BpuEI CTTGAG 4 cut(s) 437, 464, 3177, 3230
BpuMI CCSGG 2 cut(s) 199, 2792
BsaAI YACGTR 2 cut(s) 1129, 1676
BsaBI GATNNNNATC 2 cut(s) 727, 1230
BsaJI CCNNGG 7 cut(s) 198, 315, 437, 916, 2126, 2454, 3137
BsaWI WCCGGW 2 cut(s) 2406, 3283
BsaXI ACNNNNNCTCC 2 cut(s) 1009, 1039
Bsc4I CCNNNNNNNGG 6 cut(s) 374, 539, 918, 1744, 3086, 3271
Bse118I RCCGGY 1 cut(s) 3181
Bse1I ACTGG 7 cut(s) 853, 867, 879, 1927, 1958, 2714, 3330
Bse21I CCTNAGG 1 cut(s) 42
Bse3DI GCAATG 3 cut(s) 1706, 1799, 2090
Bse8I GATNNNNATC 2 cut(s) 727, 1230
BseAI TCCGGA 1 cut(s) 3283
BseBI CCWGG 1 cut(s) 1155
BseDI CCNNGG 7 cut(s) 198, 315, 437, 916, 2126, 2454, 3137
BseJI GATNNNNATC 2 cut(s) 727, 1230
BseLI CCNNNNNNNGG 6 cut(s) 374, 539, 918, 1744, 3086, 3271
BseMI GCAATG 3 cut(s) 1706, 1799, 2090
BseMII CTCAG 8 cut(s) 180, 594, 978, 1572, 2223, 2311, 2601, 3230
BseNI ACTGG 7 cut(s) 853, 867, 879, 1927, 1958, 2714, 3330
BseRI GAGGAG 1 cut(s) 3290
BseSI GKGCMC 1 cut(s) 1867
Bsh1236I CGCG 3 cut(s) 317, 2245, 3203
BshFI GGCC 4 cut(s) 442, 1507, 1627, 2975
BshNI GGYRCC 3 cut(s) 1473, 2131, 2204
BsiHKAI GWGCWC 2 cut(s) 1066, 1852
BsiHKCI CYCGRG 2 cut(s) 133, 2127
BsiSI CCGG 8 cut(s) 153, 198, 368, 2302, 2407, 2791, 3182, 3284
BslFI GGGAC 1 cut(s) 2807
BslI CCNNNNNNNGG 6 cut(s) 374, 539, 918, 1744, 3086, 3271
BsmAI GTCTC 4 cut(s) 692, 1495, 1551, 2255
BsmBI CGTCTC 1 cut(s) 1495
BsmFI GGGAC 1 cut(s) 2807
BsmI GAATGC 2 cut(s) 347, 2770
BsnI GGCC 4 cut(s) 442, 1507, 1627, 2975
BsoBI CYCGRG 2 cut(s) 133, 2127
Bsp119I TTCGAA 2 cut(s) 2218, 2326
Bsp1286I GDGCHC 3 cut(s) 1066, 1852, 1867
Bsp13I TCCGGA 1 cut(s) 3283
Bsp1720I GCTNAGC 1 cut(s) 964
Bsp19I CCATGG 1 cut(s) 2454
BspANI GGCC 4 cut(s) 442, 1507, 1627, 2975
BspCNI CTCAG 8 cut(s) 181, 595, 977, 1573, 2222, 2310, 2602, 3231
BspEI TCCGGA 1 cut(s) 3283
BspFNI CGCG 3 cut(s) 317, 2245, 3203
BspHI TCATGA 1 cut(s) 3109
BspMAI CTGCAG 1 cut(s) 1791
BspMI ACCTGC 2 cut(s) 1922, 2274
BspPI GGATC 3 cut(s) 1103, 2261, 2428
BspT104I TTCGAA 2 cut(s) 2218, 2326
BspT107I GGYRCC 3 cut(s) 1473, 2131, 2204
BsrDI GCAATG 3 cut(s) 1706, 1799, 2090
BsrFI RCCGGY 1 cut(s) 3181
BsrI ACTGG 7 cut(s) 853, 867, 879, 1927, 1958, 2714, 3330
BssAI RCCGGY 1 cut(s) 3181
BssECI CCNNGG 7 cut(s) 198, 315, 437, 916, 2126, 2454, 3137
BssT1I CCWWGG 3 cut(s) 916, 2454, 3137
Bst2UI CCWGG 1 cut(s) 1155
Bst4CI ACNGT 6 cut(s) 85, 998, 1049, 1088, 1526, 2504
Bst6I CTCTTC 2 cut(s) 84, 2828
BstBAI YACGTR 2 cut(s) 1129, 1676
BstBI TTCGAA 2 cut(s) 2218, 2326
BstC8I GCNNGC 6 cut(s) 347, 600, 1806, 2991, 3050, 3199
BstDSI CCRYGG 2 cut(s) 315, 2454
BstFNI CGCG 3 cut(s) 317, 2245, 3203
BstHHI GCGC 1 cut(s) 3203
BstMAI GTCTC 4 cut(s) 692, 1495, 1551, 2255
BstNI CCWGG 1 cut(s) 1155
BstNSI RCATGY 5 cut(s) 2177, 2238, 2754, 2855, 2913
BstPAI GACNNNNGTC 1 cut(s) 2706
BstSCI CCNGG 3 cut(s) 197, 1153, 2790
BstSFI CTRYAG 3 cut(s) 888, 1787, 1891
BstSLI GKGCMC 1 cut(s) 1867
BstUI CGCG 3 cut(s) 317, 2245, 3203
BstV2I GAAGAC 1 cut(s) 2702
BstX2I RGATCY 2 cut(s) 568, 1165
BstXI CCANNNNNNTGG 1 cut(s) 2178
BstYI RGATCY 2 cut(s) 568, 1165
Bsu36I CCTNAGG 1 cut(s) 42
BsuI GTATCC 1 cut(s) 731
BsuRI GGCC 4 cut(s) 442, 1507, 1627, 2975
BtgI CCRYGG 2 cut(s) 315, 2454
BtgZI GCGATG 4 cut(s) 135, 335, 2719, 2902
BtsI GCAGTG 1 cut(s) 3000
BtsIMutI CAGTG 5 cut(s) 860, 1578, 2721, 3000, 3236
BveI ACCTGC 2 cut(s) 1922, 2274
Cac8I GCNNGC 6 cut(s) 347, 600, 1806, 2991, 3050, 3199
CaiI CAGNNNCTG 2 cut(s) 65, 3224
CciI TCATGA 1 cut(s) 3109
CfoI GCGC 1 cut(s) 3203
Cfr10I RCCGGY 1 cut(s) 3181
Cfr13I GGNCC 5 cut(s) 706, 1506, 1585, 2304, 2974
Cfr42I CCGCGG 1 cut(s) 318
CseI GACGC 1 cut(s) 2696
Csp6I GTAC 8 cut(s) 787, 1045, 1241, 2132, 2448, 3082, 3092, 3270
CviQI GTAC 8 cut(s) 787, 1045, 1241, 2132, 2448, 3082, 3092, 3270
DraI TTTAAA 4 cut(s) 618, 777, 1758, 1965
DraIII CACNNNGTG 1 cut(s) 866
Eam1104I CTCTTC 2 cut(s) 84, 2828
EarI CTCTTC 2 cut(s) 84, 2828
EciI GGCGGA 1 cut(s) 117
Ecl136II GAGCTC 1 cut(s) 1064
Eco130I CCWWGG 3 cut(s) 916, 2454, 3137
Eco147I AGGCCT 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 1066
Eco32I GATATC 4 cut(s) 630, 1981, 2574, 3031
Eco47I GGWCC 3 cut(s) 706, 1585, 2304
Eco53kI GAGCTC 1 cut(s) 1064
Eco57I CTGAAG 5 cut(s) 87, 296, 767, 1212, 1422
Eco72I CACGTG 2 cut(s) 1129, 1676
Eco81I CCTNAGG 1 cut(s) 42
Eco88I CYCGRG 2 cut(s) 133, 2127
EcoICRI GAGCTC 1 cut(s) 1064
EcoRI GAATTC 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 1153
EcoRV GATATC 4 cut(s) 630, 1981, 2574, 3031
EcoT14I CCWWGG 3 cut(s) 916, 2454, 3137
EcoT22I ATGCAT 3 cut(s) 1387, 1447, 2236
EcoT38I GRGCYC 1 cut(s) 1066
ErhI CCWWGG 3 cut(s) 916, 2454, 3137
Esp3I CGTCTC 1 cut(s) 1495
FaqI GGGAC 1 cut(s) 2807
FauI CCCGC 1 cut(s) 1125
FriOI GRGCYC 1 cut(s) 1066
FspBI CTAG 7 cut(s) 566, 734, 1248, 1338, 1346, 1518, 1640
GlaI GCGC 1 cut(s) 3202
GsuI CTGGAG 3 cut(s) 516, 752, 1314
HaeIII GGCC 4 cut(s) 442, 1507, 1627, 2975
HapII CCGG 8 cut(s) 153, 198, 368, 2302, 2407, 2791, 3182, 3284
HgaI GACGC 1 cut(s) 2696
HhaI GCGC 1 cut(s) 3203
Hin6I GCGC 1 cut(s) 3201
HinP1I GCGC 1 cut(s) 3201
HincII GTYRAC 4 cut(s) 1463, 3066, 3144, 3253
HindII GTYRAC 4 cut(s) 1463, 3066, 3144, 3253
HindIII AAGCTT 2 cut(s) 160, 794
HpaI GTTAAC 1 cut(s) 3144
HpaII CCGG 8 cut(s) 153, 198, 368, 2302, 2407, 2791, 3182, 3284
HphI GGTGA 6 cut(s) 481, 878, 1427, 1759, 2711, 2780
Hpy166II GTNNAC 6 cut(s) 1463, 1693, 1950, 3066, 3144, 3253
Hpy8I GTNNAC 6 cut(s) 1463, 1693, 1950, 3066, 3144, 3253
Hpy99I CGWCG 4 cut(s) 119, 367, 2034, 3301
HpyCH4III ACNGT 6 cut(s) 85, 998, 1049, 1088, 1526, 2504
HpyCH4IV ACGT 5 cut(s) 222, 1128, 1675, 1729, 2796
HpySE526I ACGT 5 cut(s) 222, 1128, 1675, 1729, 2796
HspAI GCGC 1 cut(s) 3201
Kpn2I TCCGGA 1 cut(s) 3283
KpnI GGTACC 1 cut(s) 2135
KspAI GTTAAC 1 cut(s) 3144
KspI CCGCGG 1 cut(s) 318
LmnI GCTCC 6 cut(s) 104, 412, 1297, 2122, 2344, 2764
MaeI CTAG 7 cut(s) 566, 734, 1248, 1338, 1346, 1518, 1640
MaeII ACGT 5 cut(s) 222, 1128, 1675, 1729, 2796
MaeIII GTNAC 7 cut(s) 506, 1122, 1324, 1619, 2153, 2392, 2699
MflI RGATCY 2 cut(s) 568, 1165
MhlI GDGCHC 3 cut(s) 1066, 1852, 1867
MlyI GAGTC 6 cut(s) 125, 1321, 1328, 1664, 3061, 3184
MmeI TCCRAC 6 cut(s) 104, 369, 675, 1695, 3036, 3320
Mph1103I ATGCAT 3 cut(s) 1387, 1447, 2236
MroI TCCGGA 1 cut(s) 3283
MroXI GAANNNNTTC 1 cut(s) 1683
MslI CAYNNNNRTG 4 cut(s) 826, 1479, 1688, 2176
MspA1I CMGCKG 2 cut(s) 110, 317
MspI CCGG 8 cut(s) 153, 198, 368, 2302, 2407, 2791, 3182, 3284
MspR9I CCNGG 3 cut(s) 199, 1155, 2792
Mva1269I GAATGC 2 cut(s) 347, 2770
MvaI CCWGG 1 cut(s) 1155
MvnI CGCG 3 cut(s) 317, 2245, 3203
NciI CCSGG 2 cut(s) 199, 2792
NcoI CCATGG 1 cut(s) 2454
NmeAIII GCCGAG 2 cut(s) 454, 462
NmuCI GTSAC 4 cut(s) 506, 1324, 1619, 2699
NsiI ATGCAT 3 cut(s) 1387, 1447, 2236
NspI RCATGY 5 cut(s) 2177, 2238, 2754, 2855, 2913
NspV TTCGAA 2 cut(s) 2218, 2326
OliI CACNNNNGTG 1 cut(s) 2176
PaeR7I CTCGAG 1 cut(s) 133
PagI TCATGA 1 cut(s) 3109
PaqCI CACCTGC 1 cut(s) 1922
PceI AGGCCT 1 cut(s) 442
PciI ACATGT 1 cut(s) 2851
PctI GAATGC 2 cut(s) 347, 2770
PdmI GAANNNNTTC 1 cut(s) 1683
PfeI GAWTC 7 cut(s) 872, 1181, 1750, 2215, 3106, 3280, 3289
PflFI GACNNNGTC 1 cut(s) 676
PflMI CCANNNNNTGG 2 cut(s) 539, 1744
PleI GAGTC 6 cut(s) 125, 1321, 1328, 1664, 3061, 3184
PmaCI CACGTG 2 cut(s) 1129, 1676
PmlI CACGTG 2 cut(s) 1129, 1676
PpsI GAGTC 6 cut(s) 125, 1321, 1328, 1664, 3061, 3184
Ppu21I YACGTR 2 cut(s) 1129, 1676
PscI ACATGT 1 cut(s) 2851
PshAI GACNNNNGTC 1 cut(s) 2706
PshBI ATTAAT 1 cut(s) 297
Psp124BI GAGCTC 1 cut(s) 1066
Psp6I CCWGG 1 cut(s) 1153
PspCI CACGTG 2 cut(s) 1129, 1676
PspGI CCWGG 1 cut(s) 1153
PspPI GGNCC 5 cut(s) 706, 1506, 1585, 2304, 2974
PspXI VCTCGAGB 1 cut(s) 133
PsrI GAACNNNNNNTAC 2 cut(s) 2480, 2512
PstI CTGCAG 1 cut(s) 1791
PstNI CAGNNNCTG 2 cut(s) 65, 3224
PsuI RGATCY 2 cut(s) 568, 1165
PsyI GACNNNGTC 1 cut(s) 676
RsaI GTAC 8 cut(s) 788, 1046, 1242, 2133, 2449, 3083, 3093, 3271
RsaNI GTAC 8 cut(s) 787, 1045, 1241, 2132, 2448, 3082, 3092, 3270
RseI CAYNNNNRTG 4 cut(s) 826, 1479, 1688, 2176
SacI GAGCTC 1 cut(s) 1066
SacII CCGCGG 1 cut(s) 318
Sau96I GGNCC 5 cut(s) 706, 1506, 1585, 2304, 2974
SchI GAGTC 6 cut(s) 125, 1321, 1328, 1664, 3061, 3184
ScrFI CCNGG 3 cut(s) 199, 1155, 2792
SduI GDGCHC 3 cut(s) 1066, 1852, 1867
SfcI CTRYAG 3 cut(s) 888, 1787, 1891
Sfr274I CTCGAG 1 cut(s) 133
Sfr303I CCGCGG 1 cut(s) 318
SfuI TTCGAA 2 cut(s) 2218, 2326
SgrBI CCGCGG 1 cut(s) 318
SinI GGWCC 3 cut(s) 706, 1585, 2304
SlaI CTCGAG 1 cut(s) 133
SmiI ATTTAAAT 1 cut(s) 618
SmiMI CAYNNNNRTG 4 cut(s) 826, 1479, 1688, 2176
SmlI CTYRAG 5 cut(s) 133, 416, 479, 3192, 3209
SmoI CTYRAG 5 cut(s) 133, 416, 479, 3192, 3209
SseBI AGGCCT 1 cut(s) 442
SspI AATATT 3 cut(s) 1270, 2185, 2276
SspMI CTAG 7 cut(s) 566, 734, 1248, 1338, 1346, 1518, 1640
SstI GAGCTC 1 cut(s) 1066
StuI AGGCCT 1 cut(s) 442
StyD4I CCNGG 3 cut(s) 197, 1153, 2790
StyI CCWWGG 3 cut(s) 916, 2454, 3137
SwaI ATTTAAAT 1 cut(s) 618
TaaI ACNGT 6 cut(s) 85, 998, 1049, 1088, 1526, 2504
TaiI ACGT 5 cut(s) 225, 1131, 1678, 1732, 2799
TaqII GACCGA 1 cut(s) 3156
TatI WGTACW 2 cut(s) 786, 1240
TauI GCSGC 3 cut(s) 110, 320, 436
TfiI GAWTC 7 cut(s) 872, 1181, 1750, 2215, 3106, 3280, 3289
TscAI CASTG 5 cut(s) 867, 1585, 2721, 3000, 3243
TseFI GTSAC 4 cut(s) 506, 1324, 1619, 2699
Tsp45I GTSAC 4 cut(s) 506, 1324, 1619, 2699
TspGWI ACGGA 4 cut(s) 2380, 2489, 3069, 3287
TspRI CASTG 5 cut(s) 867, 1585, 2721, 3000, 3243
Tth111I GACNNNGTC 1 cut(s) 676
Van91I CCANNNNNTGG 2 cut(s) 539, 1744
VpaK11BI GGWCC 3 cut(s) 706, 1585, 2304
VspI ATTAAT 1 cut(s) 297
XapI RAATTY 5 cut(s) 184, 1658, 1969, 2467, 3075
XbaI TCTAGA 1 cut(s) 565
XceI RCATGY 5 cut(s) 2177, 2238, 2754, 2855, 2913
XcmI CCANNNNNNNNNTGG 1 cut(s) 1595
XhoI CTCGAG 1 cut(s) 133
XmnI GAANNNNTTC 1 cut(s) 1683
XspI CTAG 7 cut(s) 566, 734, 1248, 1338, 1346, 1518, 1640
Zsp2I ATGCAT 3 cut(s) 1387, 1447, 2236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.