pycom13g18250

Transcription factor that specifically binds AT-rich DNA sequences related to the nuclear matrix attachment regions (MARs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004329
Physical Location & Seq
Reverse (-)
14342619 .. 14343485
867 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 867 bp
ATGGACCCGGTAGTCACCAATTCGCCAACTCTGAGCAAGCAGCGGGAGCTCGAAATTTCCATAAACGAAAACAACAGCGGTAGAAGCAGTGGCAGAGACGATGATGACGACAGAGACCATGATGAGCCCAAAGAAGGCGCAGTCGAGATTGGATCCCGCAGGCCAAGAGGTCGCCCTCCAGGATCCAAAAACAAGCCCAAACCCCCCATCTTCGTCACCCGGGACAGCCCCAACTCCCTCCGTAGTCACGTCATGGAGGTTGCCGGCGGCGCTGACGTGGCGGAGAGCGTGGCGCAGTTCGCCAGGAGGCGGCAGAGAGGCGTGTGTGTTCTCAGCGGGAGTGGCTCGGTCGCCAACGTAACCCTCAGACAACCTGCGGCTCCAGGAGCTGTGGTGGCGCTTCACGGGAGGTTTGAAATTTTGTCTCTGAGTGGGGCGTTCCTTCCTGGTCCTGCCCCTCCCGGATCTACTGGGCTGACGGTTTATTTAGCCGGTGGGCAGGGGCAGGTGGTTGGAGGAAGTGTGGTGGGGTCACTAGTGGCGGCAGGCCCAGTGATGGTGATGGCAGCAACATTTGCAAATGCTACTTATGAGAGGCTGCCTCTTGAGGAGGATGAGGAGGGTGGAGGAAGTGGAGGAGGAGGAGGAGGGCATAATAATGGAGGTTCCCCAACTGGTGGTGGGAGCAGTGGAGCCCAATTGGGGAGTGGGGGGCATCAGCAGCAGCAGCAACTCCCTGATCCTTCATCTGGACTTCCCAATATTTACAGTCATCATCTGCCTCCAAATCTGATCCCCAATGGTGGCCATGGACATCTTGGGCATGAGGCTTATGCTAATTGGGCGCATTCAAGACCACCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

289

Amino Acids

29.46

Weight (kDa)

6.25

Isoelectric Point (pI)

56.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 81 - 193 1.4e-24 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 496
AasI GACNNNNNNGTC 1 cut(s) 11
Acc36I ACCTGC 2 cut(s) 382, 496
AccB7I CCANNNNNTGG 1 cut(s) 677
AciI CCGC 9 cut(s) 43, 78, 157, 267, 281, 310, 336, 377, 542
AclWI GGATC 7 cut(s) 147, 160, 177, 190, 472, 734, 787
AcoI YGGCCR 1 cut(s) 805
AcsI RAATTY 2 cut(s) 54, 417
AfiI CCNNNNNNNGG 7 cut(s) 134, 309, 556, 677, 702, 749, 803
AgsI TTSAA 2 cut(s) 416, 852
AhlI ACTAGT 1 cut(s) 535
AjiI CACGTC 2 cut(s) 250, 277
AjnI CCWGG 4 cut(s) 178, 302, 382, 445
AluBI AGCT 2 cut(s) 49, 389
AluI AGCT 2 cut(s) 49, 389
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 3 cut(s) 90, 108, 429
AlwI GGATC 7 cut(s) 147, 160, 177, 190, 472, 734, 787
AlwNI CAGNNNCTG 1 cut(s) 389
Ama87I CYCGRG 1 cut(s) 219
AoxI GGCC 3 cut(s) 161, 547, 805
ApeKI GCWGC 6 cut(s) 40, 566, 598, 721, 724, 727
ApoI RAATTY 2 cut(s) 54, 417
AspLEI GCGC 5 cut(s) 140, 272, 295, 400, 847
AspS9I GGNCC 3 cut(s) 4, 449, 548
AsuC2I CCSGG 4 cut(s) 8, 220, 221, 462
AsuHPI GGTGA 3 cut(s) 7, 208, 571
AvaI CYCGRG 1 cut(s) 219
AvaII GGWCC 2 cut(s) 4, 449
BalI TGGCCA 1 cut(s) 807
BamHI GGATCC 2 cut(s) 152, 182
BanII GRGCYC 3 cut(s) 51, 129, 697
Bbv12I GWGCWC 1 cut(s) 51
BbvI GCAGC 6 cut(s) 52, 578, 585, 733, 736, 739
BccI CCATC 3 cut(s) 215, 550, 556
BciT130I CCWGG 4 cut(s) 180, 304, 384, 447
BcnI CCSGG 4 cut(s) 8, 220, 221, 462
BcoDI GTCTC 3 cut(s) 90, 108, 429
BcuI ACTAGT 1 cut(s) 535
BfaI CTAG 1 cut(s) 536
BfoI RGCGCY 2 cut(s) 273, 401
BfuAI ACCTGC 2 cut(s) 382, 496
Bme1390I CCNGG 8 cut(s) 8, 180, 220, 221, 304, 384, 447, 462
Bme18I GGWCC 2 cut(s) 4, 449
BmeT110I CYCGRG 1 cut(s) 219
BmgBI CACGTC 2 cut(s) 250, 277
BmgT120I GGNCC 3 cut(s) 4, 449, 548
BmiI GGNNCC 6 cut(s) 6, 154, 184, 381, 667, 694
BmrFI CCNGG 8 cut(s) 8, 180, 220, 221, 304, 384, 447, 462
BmrI ACTGGG 2 cut(s) 480, 545
BmsI GCATC 1 cut(s) 724
BmuI ACTGGG 2 cut(s) 480, 545
BplI GAGNNNNNCTC 2 cut(s) 586, 618
BpmI CTGGAG 2 cut(s) 162, 366
BpuEI CTTGAG 1 cut(s) 626
BpuMI CCSGG 4 cut(s) 8, 220, 221, 462
BsaI GGTCTC 1 cut(s) 108
BsaJI CCNNGG 2 cut(s) 219, 808
BsaXI ACNNNNNCTCC 4 cut(s) 378, 408, 507, 537
Bsc4I CCNNNNNNNGG 7 cut(s) 134, 309, 556, 677, 702, 749, 803
Bse118I RCCGGY 2 cut(s) 263, 491
Bse1I ACTGG 3 cut(s) 475, 551, 679
BseBI CCWGG 4 cut(s) 180, 304, 384, 447
BseDI CCNNGG 2 cut(s) 219, 808
BseGI GGATG 1 cut(s) 619
BseLI CCNNNNNNNGG 7 cut(s) 134, 309, 556, 677, 702, 749, 803
BseMII CTCAG 4 cut(s) 23, 346, 379, 419
BseNI ACTGG 3 cut(s) 475, 551, 679
BseRI GAGGAG 6 cut(s) 623, 632, 651, 654, 657, 660
BseXI GCAGC 6 cut(s) 52, 578, 585, 733, 736, 739
Bsh1285I CGRYCG 1 cut(s) 351
BshFI GGCC 3 cut(s) 163, 549, 807
BsiEI CGRYCG 1 cut(s) 351
BsiHKAI GWGCWC 1 cut(s) 51
BsiHKCI CYCGRG 1 cut(s) 219
BsiSI CCGG 5 cut(s) 8, 220, 264, 462, 492
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 7 cut(s) 134, 309, 556, 677, 702, 749, 803
BsmAI GTCTC 3 cut(s) 90, 108, 429
BsmBI CGTCTC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 236
BsmI GAATGC 1 cut(s) 847
BsnI GGCC 3 cut(s) 163, 549, 807
Bso31I GGTCTC 1 cut(s) 108
BsoBI CYCGRG 1 cut(s) 219
Bsp1286I GDGCHC 3 cut(s) 51, 129, 697
Bsp143I GATC 5 cut(s) 152, 182, 464, 739, 792
Bsp19I CCATGG 1 cut(s) 808
BspACI CCGC 9 cut(s) 43, 78, 157, 267, 281, 310, 336, 377, 542
BspANI GGCC 3 cut(s) 163, 549, 807
BspCNI CTCAG 4 cut(s) 24, 345, 378, 420
BspLI GGNNCC 6 cut(s) 6, 154, 184, 381, 667, 694
BspMI ACCTGC 2 cut(s) 382, 496
BspPI GGATC 7 cut(s) 147, 160, 177, 190, 472, 734, 787
BspTNI GGTCTC 1 cut(s) 108
BsrFI RCCGGY 2 cut(s) 263, 491
BsrI ACTGG 3 cut(s) 475, 551, 679
BssAI RCCGGY 2 cut(s) 263, 491
BssECI CCNNGG 2 cut(s) 219, 808
BssMI GATC 5 cut(s) 152, 182, 464, 739, 792
BssT1I CCWWGG 1 cut(s) 808
Bst2UI CCWGG 4 cut(s) 180, 304, 384, 447
Bst4CI ACNGT 2 cut(s) 481, 770
BstAPI GCANNNNNTGC 1 cut(s) 575
BstC8I GCNNGC 4 cut(s) 38, 161, 265, 547
BstDEI CTNAG 4 cut(s) 32, 332, 365, 428
BstDSI CCRYGG 1 cut(s) 808
BstF5I GGATG 1 cut(s) 619
BstH2I RGCGCY 2 cut(s) 273, 401
BstHHI GCGC 5 cut(s) 140, 272, 295, 400, 847
BstKTI GATC 5 cut(s) 155, 185, 467, 742, 795
BstMAI GTCTC 3 cut(s) 90, 108, 429
BstMBI GATC 5 cut(s) 152, 182, 464, 739, 792
BstMCI CGRYCG 1 cut(s) 351
BstNI CCWGG 4 cut(s) 180, 304, 384, 447
BstSCI CCNGG 8 cut(s) 6, 178, 218, 219, 302, 382, 445, 460
BstV1I GCAGC 6 cut(s) 52, 578, 585, 733, 736, 739
BstX2I RGATCY 3 cut(s) 152, 182, 464
BstYI RGATCY 3 cut(s) 152, 182, 464
BsuRI GGCC 3 cut(s) 163, 549, 807
BtgI CCRYGG 1 cut(s) 808
BtrI CACGTC 2 cut(s) 250, 277
BtsCI GGATG 1 cut(s) 619
BtsI GCAGTG 2 cut(s) 94, 694
BtsIMutI CAGTG 3 cut(s) 94, 558, 694
BveI ACCTGC 2 cut(s) 382, 496
Cac8I GCNNGC 4 cut(s) 38, 161, 265, 547
CaiI CAGNNNCTG 1 cut(s) 389
CfoI GCGC 5 cut(s) 140, 272, 295, 400, 847
Cfr10I RCCGGY 2 cut(s) 263, 491
Cfr13I GGNCC 3 cut(s) 4, 449, 548
Cfr9I CCCGGG 1 cut(s) 219
CviAII CATG 4 cut(s) 119, 253, 809, 824
DdeI CTNAG 4 cut(s) 32, 332, 365, 428
DpnI GATC 5 cut(s) 154, 184, 466, 741, 794
DpnII GATC 5 cut(s) 152, 182, 464, 739, 792
DrdI GACNNNNNNGTC 1 cut(s) 11
DseDI GACNNNNNNGTC 1 cut(s) 11
EaeI YGGCCR 1 cut(s) 805
EciI GGCGGA 1 cut(s) 296
Ecl136II GAGCTC 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 808
Eco24I GRGCYC 3 cut(s) 51, 129, 697
Eco31I GGTCTC 1 cut(s) 108
Eco47I GGWCC 2 cut(s) 4, 449
Eco53kI GAGCTC 1 cut(s) 49
Eco88I CYCGRG 1 cut(s) 219
EcoICRI GAGCTC 1 cut(s) 49
EcoRII CCWGG 4 cut(s) 178, 302, 382, 445
EcoT14I CCWWGG 1 cut(s) 808
EcoT38I GRGCYC 3 cut(s) 51, 129, 697
ErhI CCWWGG 1 cut(s) 808
Esp3I CGTCTC 1 cut(s) 90
FaeI CATG 4 cut(s) 122, 256, 812, 827
FaiI YATR 8 cut(s) 62, 120, 254, 591, 654, 810, 825, 834
FalI AAGNNNNNCTT 1 cut(s) 844
FaqI GGGAC 1 cut(s) 236
FatI CATG 4 cut(s) 118, 252, 808, 823
FauI CCCGC 3 cut(s) 36, 164, 329
FokI GGATG 1 cut(s) 626
FriOI GRGCYC 3 cut(s) 51, 129, 697
FspBI CTAG 1 cut(s) 536
GlaI GCGC 5 cut(s) 139, 271, 294, 399, 846
GsuI CTGGAG 2 cut(s) 162, 366
HaeII RGCGCY 2 cut(s) 273, 401
HaeIII GGCC 3 cut(s) 163, 549, 807
HapII CCGG 5 cut(s) 8, 220, 264, 462, 492
HhaI GCGC 5 cut(s) 140, 272, 295, 400, 847
Hin1II CATG 4 cut(s) 122, 256, 812, 827
Hin6I GCGC 5 cut(s) 138, 270, 293, 398, 845
HinP1I GCGC 5 cut(s) 138, 270, 293, 398, 845
HpaII CCGG 5 cut(s) 8, 220, 264, 462, 492
HphI GGTGA 3 cut(s) 7, 208, 571
Hpy188I TCNGA 4 cut(s) 33, 368, 429, 792
Hpy188III TCNNGA 4 cut(s) 145, 605, 750, 852
HpyAV CCTTC 3 cut(s) 128, 452, 753
HpyCH4III ACNGT 2 cut(s) 481, 770
HpyCH4IV ACGT 3 cut(s) 249, 276, 357
HpyCH4V TGCA 1 cut(s) 578
HpyF3I CTNAG 4 cut(s) 32, 332, 365, 428
HpySE526I ACGT 3 cut(s) 249, 276, 357
Hsp92II CATG 4 cut(s) 122, 256, 812, 827
HspAI GCGC 5 cut(s) 138, 270, 293, 398, 845
KroI GCCGGC 1 cut(s) 263
KroNI GCCGGC 1 cut(s) 265
Kzo9I GATC 5 cut(s) 152, 182, 464, 739, 792
LmnI GCTCC 5 cut(s) 46, 385, 386, 684, 692
Lsp1109I GCAGC 6 cut(s) 52, 578, 585, 733, 736, 739
LweI GCATC 1 cut(s) 724
MaeI CTAG 1 cut(s) 536
MaeII ACGT 3 cut(s) 249, 276, 357
MaeIII GTNAC 5 cut(s) 13, 214, 245, 358, 531
MalI GATC 5 cut(s) 154, 184, 466, 741, 794
MboI GATC 5 cut(s) 152, 182, 464, 739, 792
MboII GAAGA 1 cut(s) 202
MfeI CAATTG 1 cut(s) 698
MflI RGATCY 3 cut(s) 152, 182, 464
MhlI GDGCHC 3 cut(s) 51, 129, 697
MlsI TGGCCA 1 cut(s) 807
MluCI AATT 5 cut(s) 19, 54, 417, 698, 838
MluNI TGGCCA 1 cut(s) 807
MmeI TCCRAC 1 cut(s) 493
Mox20I TGGCCA 1 cut(s) 807
MroNI GCCGGC 1 cut(s) 263
MscI TGGCCA 1 cut(s) 807
MslI CAYNNNNRTG 1 cut(s) 657
Msp20I TGGCCA 1 cut(s) 807
MspA1I CMGCKG 3 cut(s) 43, 78, 336
MspI CCGG 5 cut(s) 8, 220, 264, 462, 492
MspR9I CCNGG 8 cut(s) 8, 180, 220, 221, 304, 384, 447, 462
MunI CAATTG 1 cut(s) 698
Mva1269I GAATGC 1 cut(s) 847
MvaI CCWGG 4 cut(s) 180, 304, 384, 447
NaeI GCCGGC 1 cut(s) 265
NciI CCSGG 4 cut(s) 8, 220, 221, 462
NcoI CCATGG 1 cut(s) 808
NdeII GATC 5 cut(s) 152, 182, 464, 739, 792
NgoMIV GCCGGC 1 cut(s) 263
NlaIII CATG 4 cut(s) 122, 256, 812, 827
NlaIV GGNNCC 6 cut(s) 6, 154, 184, 381, 667, 694
NmuCI GTSAC 4 cut(s) 13, 214, 245, 531
PaqCI CACCTGC 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PctI GAATGC 1 cut(s) 847
PdiI GCCGGC 1 cut(s) 265
PflMI CCANNNNNTGG 1 cut(s) 677
PfoI TCCNGGA 3 cut(s) 178, 382, 460
Psp124BI GAGCTC 1 cut(s) 51
Psp6I CCWGG 4 cut(s) 178, 302, 382, 445
PspGI CCWGG 4 cut(s) 178, 302, 382, 445
PspN4I GGNNCC 6 cut(s) 6, 154, 184, 381, 667, 694
PspPI GGNCC 3 cut(s) 4, 449, 548
PstNI CAGNNNCTG 1 cut(s) 389
PsuI RGATCY 3 cut(s) 152, 182, 464
RseI CAYNNNNRTG 1 cut(s) 657
SacI GAGCTC 1 cut(s) 51
Sau3AI GATC 5 cut(s) 152, 182, 464, 739, 792
Sau96I GGNCC 3 cut(s) 4, 449, 548
ScrFI CCNGG 8 cut(s) 8, 180, 220, 221, 304, 384, 447, 462
SduI GDGCHC 3 cut(s) 51, 129, 697
SfaNI GCATC 1 cut(s) 724
SinI GGWCC 2 cut(s) 4, 449
SmaI CCCGGG 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 657
SmlI CTYRAG 1 cut(s) 605
SmoI CTYRAG 1 cut(s) 605
SpeI ACTAGT 1 cut(s) 535
Sse9I AATT 5 cut(s) 19, 54, 417, 698, 838
SsiI CCGC 9 cut(s) 43, 78, 157, 267, 281, 310, 336, 377, 542
SspI AATATT 1 cut(s) 763
SspMI CTAG 1 cut(s) 536
SstI GAGCTC 1 cut(s) 51
StyD4I CCNGG 8 cut(s) 6, 178, 218, 219, 302, 382, 445, 460
StyI CCWWGG 1 cut(s) 808
TaaI ACNGT 2 cut(s) 481, 770
TaiI ACGT 3 cut(s) 252, 279, 360
TaqI TCGA 2 cut(s) 51, 144
TaqII GACCGA 1 cut(s) 337
TasI AATT 5 cut(s) 19, 54, 417, 698, 838
TauI GCSGC 4 cut(s) 270, 313, 380, 545
TscAI CASTG 3 cut(s) 94, 558, 694
TseFI GTSAC 4 cut(s) 13, 214, 245, 531
TseI GCWGC 6 cut(s) 40, 566, 598, 721, 724, 727
Tsp45I GTSAC 4 cut(s) 13, 214, 245, 531
TspDTI ATGAA 1 cut(s) 735
TspGWI ACGGA 1 cut(s) 230
TspMI CCCGGG 1 cut(s) 219
TspRI CASTG 3 cut(s) 94, 558, 694
Van91I CCANNNNNTGG 1 cut(s) 677
VpaK11BI GGWCC 2 cut(s) 4, 449
XapI RAATTY 2 cut(s) 54, 417
XcmI CCANNNNNNNNNTGG 2 cut(s) 704, 815
XmaI CCCGGG 1 cut(s) 219
XspI CTAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.