Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0004747

Physical Location & Seq

Genomic Coordinates Forward (+)

14884547 .. 14886886

Gene Structure 2340 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2340 bp

ATGAGCTCCGCCTCCATCCAAACACTGCCAAAAACCCTCTTCAAGAACCCCAGCACCATCAAATGCCAATCCCATGCCAAACAGCTCCACGCCCAGATCCTCAAATCCAACTGCGGCCCTTCGGCTCCCGACCTCTCCTTTGTCCTCTCTGTTTACTCCACCCTGAACCTCCTACACGACTCCCTCACCCTCTTCAACTCCCTCCCTTCTCCTCCCCCCACTCTCGCTTGGAAGTCGGTTATCCGATGCTACGCCGCCCACGGCCTCTGCCACCGTTCCTTGGCCTCCTTTGTGGAAATGATGGGTTTTGGGATATACCCAGATCACAATGTGTTCCCTTCTGTGCTCAAATCATGCGCATTGCTTGGGGACTTGAGGTTGGGCGCGTCCGTCCATGGGTGTGTTGTTCGGTTCGGTATGGATTGTGATTTGTATACTTGTAATGCACTTATGAATATGTACGCAAAGTTAGAGACTTTGGAGGGGATTGGTGGGCAGAGGTTTGGTGCGCCCAAGTTGTTTGATAGAATGCCTCAACGAAATCAAGTTTGTAGAATTGGGTCGTTGGAGGTAGAAGGTGAGGCTTTGTGTAGTAGTCAATCAAATAAACTGGTTAATGACTTGCAGATGCAGGGTAATGGTATTGATAGGACTGTTGGTTTGACTACATATGTAGATGTAGACCCTCCACTTCGCGCATACCGGCAATTCAATGATCAGTGTCATAAAGCTTTCGAGCAAGAGGAAGGGAAAAGAGTTGCCAGAGTGGATAGTGTGAGAAAGGTCTTTAACTTGATGCAAAAAAGAGATATTGTTTCCTGGAATACAGTCATTGCTGGGAATGCACAGAATGGGATGTATCGAGAAGCTTTGGCAATGGTCAGACATATGGGGAATGCTGACTTGAAGCCCGATGCTTTTACTTTGTCTAGTGTCCTTCCAGTTTTTGCAGAATATGTAGATGTTATCAAGGGAAAGGAGATTCATGGGTATGCTATAAGACGTGGACTTGATGCAGATGTATTCATTGGGAGTAGCTTAATTGACATGTATGCCAATTGCACTCGAGTGCAAGATTCCCTGCAGGTGTTCAACCTGCTACCTAAGCGTGATGCCATTTCATGGAACTCGATCATTGCAGGATGTGTGCAGAACGGTATGTTTGATGAAGGACTGATATTCTTTCGCGAGATGTTGATGAGTAAAGTTAAGCCTGTGCATGTATCCTTTTCAAGTATTATTCCAGCTTGCGCTCACCTGACAACTCTACATTTGGGAAAGCAGCTCCATGGATACATAACTAGGGGTGGATTCAAGGATAATGTGTTTGTAGCGAGCTCACTGGTGGACATGTACGCCAAATGCGGTAACATTAGGATGGCAAGGTGGATTTTTCACAAAATGGAGAAACATGACATGGTGTCATGGACAGCCATGATTATGGGATATGCTTTGCATGGTCATGCACATGATGCCATTTCCTCTTTTGAGCAGATGAAAGAAGAGGGCATAAAGCCCAACTATGTTTCTTTCATGGCTGTATTGACCGCATGTAGCCATGCTGGATTGGTAGACAAAGCTTGGAAGTATTTTAATAGTATGACTGGGGAATATGGGATTGCTCCCGGTATAGAGCACTATGCTGCTGTTGCAGACGTTCTTGGTCGAGCTGGAAGGTTGGAGGAAGCTTATCAGTTTATCTCTACCATGCATATATTACCGACGGGAAGTGTATGGTCAACATTGTTGGCTGCTTGTAGAGTTCACAAGAAACTTGAATTAGCTGAGAAGGTTGCTGAGAAAATATTTACAGTTGATCCTGGGAACATGGGTGCTTATGTTCTATTGTCAAACACATATTCGGCTGCTAAGAGGTGGAAAGATGCAGCAAAATTGAGGATCACTATGAGGGATAAGGGATTGAAAAAGAAGCCAGCCTGCAGCTGGATTGAAGTTAAAAGCAAGGTTCATGCTTTTGTGGCGGGAGATAAATCCCACCCACTTTATGATCGAATAATTGAGGCTCTAGGAGTCATTTTGGAGCAGATGGAACGAGAAGGGTATGCGCCCAACACAAATGAGGTGCTCCATGATGTTGAAGAGGAGCAAAAGAAGTACTTATTGTGTCACCACAGTGAAAGGCTTGCCATAGGATTTGGCATCATTAGCACTCCTGCTGGGACAACAATTCGAGTAACAAAGAACATTAGGGTATGCGTGGACTGCCACATAGCAATAAAATTTATGTCAAAGATCGTTGGGAGGGAGTTCATTGTCAGGGATAACAGCCGGTTTCACCATTTCAAGGGTGGAGAGTGTTCGTGTGGAGATTACTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

780

Amino Acids

86.83

Weight (kDa)

8.74

Isoelectric Point (pI)

38.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	269 - 315	9.7e-08	PPR repeat family
PPR_2	PF13041	371 - 419	5.9e-08	PPR repeat family
PPR	PF01535	373 - 401	3.2e-06	PPR repeat
PPR_3	PF13812	471 - 518	1.4e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	472 - 519	5.7e-09	PPR repeat family
PPR	PF01535	474 - 504	2.3e-06	PPR repeat
E_motif	PF20431	589 - 651	6.2e-19	E motif
DYW_deaminase	PF14432	687 - 779	8.2e-42	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004747)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23330 AT3G23330
fragaria_vesca	FvH4_4g03820 FvH4_4g04640
malus_domestica	MD13G1215400.v1.1 MD15G1316700.v1.1 MD16G1226500.v1.1
prunus_persica	Prupe.1G040600_v2.0.a1 Prupe.1G048700_v2.0.a1
pyrus_communis	pycom13g18860
rosa_chinensis	RchiOBHm_Chr4g0393661 RchiOBHm_Chr4g0395421
rosa_laevigata	RLG00000009623 RLG00000009737
rosa_multiflora	Rmu_sc0004598.1_g000006 Rmu_sc0010124.1_g000005 Rmu_sc0010817.1_g000005
rosa_roxburghii	Rroxscaffold_5G00338850 Rroxscaffold_5G00340380
rosa_rugosa	Rorug03G0357200 Rorug03G0371800.1
rosa_samantha	Rh4AG048500 Rh4AG059800 Rh4BG044900 Rh4BG058400 Rh4CG052000 Rh4CG064100
rosa_wichuraiana	Rw0G003320 Rw0G022520 Rw4G003850 Rw4G004790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1075
Acc16I	TGCGCA	1 cut(s)	358
Acc36I	ACCTGC	2 cut(s)	1075, 1104
AccB7I	CCANNNNNTGG	1 cut(s)	1122
AccI	GTMKAC	3 cut(s)	434, 681, 1572
AccII	CGCG	3 cut(s)	386, 696, 1188
AciI	CCGC	6 cut(s)	9, 114, 255, 1365, 1548, 1982
AclWI	GGATC	3 cut(s)	91, 1811, 1907
AcsI	RAATTY	1 cut(s)	2240
AdeI	CACNNNGTG	1 cut(s)	331
AfaI	GTAC	3 cut(s)	461, 1355, 2117
AfiI	CCNNNNNNNGG	5 cut(s)	280, 396, 1122, 1944, 2305
AflIII	ACRYGT	2 cut(s)	1047, 1350
AhdI	GACNNNNNGTC	1 cut(s)	1420
AjiI	CACGTC	1 cut(s)	1004
AjnI	CCWGG	2 cut(s)	818, 1819
AleI	CACNNNNGTG	2 cut(s)	1067, 2133
Alw21I	GWGCWC	5 cut(s)	8, 348, 1340, 1638, 2088
Alw26I	GTCTC	1 cut(s)	467
AlwI	GGATC	3 cut(s)	91, 1811, 1907
Ama87I	CYCGRG	1 cut(s)	1065
AoxI	GGCC	3 cut(s)	115, 262, 282
ApeKI	GCWGC	6 cut(s)	1282, 1643, 1751, 1865, 1886, 1941
ApoI	RAATTY	1 cut(s)	2240
ArsI	GACNNNNNNTTYG	4 cut(s)	122, 154, 643, 675
AspLEI	GCGC	6 cut(s)	359, 386, 511, 698, 1253, 2068
AspS9I	GGNCC	1 cut(s)	116
AsuC2I	CCSGG	1 cut(s)	1626
AsuHPI	GGTGA	5 cut(s)	178, 590, 1247, 2120, 2288
AvaI	CYCGRG	1 cut(s)	1065
BanII	GRGCYC	2 cut(s)	8, 1340
Bbv12I	GWGCWC	5 cut(s)	8, 348, 1340, 1638, 2088
BbvI	GCAGC	6 cut(s)	1294, 1630, 1738, 1852, 1898, 1953
BccI	CCATC	5 cut(s)	23, 65, 295, 1372, 2041
BceAI	ACGGC	1 cut(s)	277
BciT130I	CCWGG	2 cut(s)	820, 1821
BciVI	GTATCC	2 cut(s)	1234, 1286
BclI	TGATCA	1 cut(s)	715
BcnI	CCSGG	1 cut(s)	1626
BcoDI	GTCTC	1 cut(s)	467
BfaI	CTAG	3 cut(s)	930, 1302, 2027
BfmI	CTRYAG	2 cut(s)	1082, 1939
BfuAI	ACCTGC	2 cut(s)	1075, 1104
BfuI	GTATCC	2 cut(s)	1234, 1286
BisI	GCNGC	8 cut(s)	115, 255, 1283, 1644, 1752, 1866, 1887, 1942
BlsI	GCNGC	8 cut(s)	116, 256, 1284, 1645, 1753, 1867, 1888, 1943
BmcAI	AGTACT	1 cut(s)	2117
Bme1390I	CCNGG	3 cut(s)	820, 1626, 1821
BmeRI	GACNNNNNGTC	1 cut(s)	1420
BmeT110I	CYCGRG	1 cut(s)	1065
BmgBI	CACGTC	1 cut(s)	1004
BmgT120I	GGNCC	1 cut(s)	116
BmiI	GGNNCC	1 cut(s)	126
BmrFI	CCNGG	3 cut(s)	820, 1626, 1821
BmrI	ACTGGG	1 cut(s)	1614
BmsI	GCATC	9 cut(s)	236, 618, 786, 904, 1003, 1102, 1462, 1873, 2169
BmuI	ACTGGG	1 cut(s)	1614
Bpu10I	CCTNAGC	1 cut(s)	1104
BpuEI	CTTGAG	1 cut(s)	394
BpuMI	CCSGG	1 cut(s)	1626
BsaJI	CCNNGG	5 cut(s)	259, 279, 394, 1288, 1820
Bsc4I	CCNNNNNNNGG	5 cut(s)	280, 396, 1122, 1944, 2305
Bse118I	RCCGGY	2 cut(s)	702, 2289
Bse1I	ACTGG	5 cut(s)	615, 941, 1347, 1609, 2339
Bse3DI	GCAATG	4 cut(s)	359, 831, 882, 1134
BseBI	CCWGG	2 cut(s)	820, 1821
BseDI	CCNNGG	5 cut(s)	259, 279, 394, 1288, 1820
BseGI	GGATG	4 cut(s)	15, 861, 1148, 1383
BseLI	CCNNNNNNNGG	5 cut(s)	280, 396, 1122, 1944, 2305
BseMI	GCAATG	4 cut(s)	359, 831, 882, 1134
BseMII	CTCAG	2 cut(s)	1776, 1788
BseNI	ACTGG	5 cut(s)	615, 941, 1347, 1609, 2339
BseRI	GAGGAG	2 cut(s)	201, 2117
BseXI	GCAGC	6 cut(s)	1294, 1630, 1738, 1852, 1898, 1953
BseYI	CCCAGC	3 cut(s)	50, 836, 2177
BsgI	GTGCAG	1 cut(s)	1169
Bsh1236I	CGCG	3 cut(s)	386, 696, 1188
BshFI	GGCC	3 cut(s)	117, 264, 284
BsiHKAI	GWGCWC	5 cut(s)	8, 348, 1340, 1638, 2088
BsiHKCI	CYCGRG	1 cut(s)	1065
BsiSI	CCGG	3 cut(s)	703, 1626, 2290
BslFI	GGGAC	2 cut(s)	383, 2194
BslI	CCNNNNNNNGG	5 cut(s)	280, 396, 1122, 1944, 2305
BsmAI	GTCTC	1 cut(s)	467
BsmFI	GGGAC	2 cut(s)	383, 2194
BsmI	GAATGC	3 cut(s)	534, 847, 901
BsnI	GGCC	3 cut(s)	117, 264, 284
BsoBI	CYCGRG	1 cut(s)	1065
Bsp1286I	GDGCHC	5 cut(s)	8, 348, 1340, 1638, 2088
Bsp143I	GATC	8 cut(s)	96, 322, 715, 1131, 1816, 1899, 2008, 2253
Bsp19I	CCATGG	2 cut(s)	394, 1288
Bsp68I	TCGCGA	1 cut(s)	1188
BspACI	CCGC	6 cut(s)	9, 114, 255, 1365, 1548, 1982
BspANI	GGCC	3 cut(s)	117, 264, 284
BspCNI	CTCAG	2 cut(s)	1777, 1789
BspFNI	CGCG	3 cut(s)	386, 696, 1188
BspLI	GGNNCC	1 cut(s)	126
BspMAI	CTGCAG	2 cut(s)	1086, 1943
BspMI	ACCTGC	2 cut(s)	1075, 1104
BspPI	GGATC	3 cut(s)	91, 1811, 1907
BsrDI	GCAATG	4 cut(s)	359, 831, 882, 1134
BsrFI	RCCGGY	2 cut(s)	702, 2289
BsrI	ACTGG	5 cut(s)	615, 941, 1347, 1609, 2339
BssAI	RCCGGY	2 cut(s)	702, 2289
BssECI	CCNNGG	5 cut(s)	259, 279, 394, 1288, 1820
BssMI	GATC	8 cut(s)	96, 322, 715, 1131, 1816, 1899, 2008, 2253
BssNAI	GTATAC	1 cut(s)	435
BssT1I	CCWWGG	3 cut(s)	279, 394, 1288
Bst1107I	GTATAC	1 cut(s)	435
Bst2UI	CCWGG	2 cut(s)	820, 1821
Bst4CI	ACNGT	6 cut(s)	275, 655, 829, 1157, 1813, 2135
Bst6I	CTCTTC	4 cut(s)	44, 197, 1497, 2094
BstAPI	GCANNNNNTGC	1 cut(s)	1472
BstC8I	GCNNGC	5 cut(s)	1249, 1336, 1935, 1939, 2145
BstDEI	CTNAG	4 cut(s)	1104, 1785, 1797, 1869
BstDSI	CCRYGG	3 cut(s)	259, 394, 1288
BstF5I	GGATG	4 cut(s)	15, 861, 1148, 1383
BstFNI	CGCG	3 cut(s)	386, 696, 1188
BstHHI	GCGC	6 cut(s)	359, 386, 511, 698, 1253, 2068
BstKTI	GATC	8 cut(s)	99, 325, 718, 1134, 1819, 1902, 2011, 2256
BstMAI	GTCTC	1 cut(s)	467
BstMBI	GATC	8 cut(s)	96, 322, 715, 1131, 1816, 1899, 2008, 2253
BstMWI	GCNNNNNNNGC	7 cut(s)	842, 1105, 1472, 1649, 1979, 2166, 2223
BstNI	CCWGG	2 cut(s)	820, 1821
BstNSI	RCATGY	4 cut(s)	1051, 1223, 1354, 1554
BstSCI	CCNGG	3 cut(s)	818, 1624, 1819
BstSFI	CTRYAG	2 cut(s)	1082, 1939
BstUI	CGCG	3 cut(s)	386, 696, 1188
BstV1I	GCAGC	6 cut(s)	1294, 1630, 1738, 1852, 1898, 1953
BstX2I	RGATCY	1 cut(s)	96
BstXI	CCANNNNNNTGG	1 cut(s)	1441
BstYI	RGATCY	1 cut(s)	96
BstZ17I	GTATAC	1 cut(s)	435
BsuI	GTATCC	2 cut(s)	1234, 1286
BsuRI	GGCC	3 cut(s)	117, 264, 284
BtgI	CCRYGG	3 cut(s)	259, 394, 1288
BtrI	CACGTC	1 cut(s)	1004
BtsCI	GGATG	4 cut(s)	15, 861, 1148, 1383
BtsI	GCAGTG	1 cut(s)	23
BtsIMutI	CAGTG	4 cut(s)	23, 725, 1340, 2140
BtuMI	TCGCGA	1 cut(s)	1188
BveI	ACCTGC	2 cut(s)	1075, 1104
Cac8I	GCNNGC	5 cut(s)	1249, 1336, 1935, 1939, 2145
CfoI	GCGC	6 cut(s)	359, 386, 511, 698, 1253, 2068
Cfr10I	RCCGGY	2 cut(s)	702, 2289
Cfr13I	GGNCC	1 cut(s)	116
CseI	GACGC	1 cut(s)	375
Csp6I	GTAC	3 cut(s)	460, 1354, 2116
CviQI	GTAC	3 cut(s)	460, 1354, 2116
DdeI	CTNAG	4 cut(s)	1104, 1785, 1797, 1869
DpnI	GATC	8 cut(s)	98, 324, 717, 1133, 1818, 1901, 2010, 2255
DpnII	GATC	8 cut(s)	96, 322, 715, 1131, 1816, 1899, 2008, 2253
DraIII	CACNNNGTG	1 cut(s)	331
DriI	GACNNNNNGTC	1 cut(s)	1420
Eam1104I	CTCTTC	4 cut(s)	44, 197, 1497, 2094
Eam1105I	GACNNNNNGTC	1 cut(s)	1420
EarI	CTCTTC	4 cut(s)	44, 197, 1497, 2094
Ecl136II	GAGCTC	2 cut(s)	6, 1338
Eco130I	CCWWGG	3 cut(s)	279, 394, 1288
Eco24I	GRGCYC	2 cut(s)	8, 1340
Eco53kI	GAGCTC	2 cut(s)	6, 1338
Eco88I	CYCGRG	1 cut(s)	1065
EcoICRI	GAGCTC	2 cut(s)	6, 1338
EcoRII	CCWGG	2 cut(s)	818, 1819
EcoT14I	CCWWGG	3 cut(s)	279, 394, 1288
EcoT22I	ATGCAT	1 cut(s)	1713
EcoT38I	GRGCYC	2 cut(s)	8, 1340
ErhI	CCWWGG	3 cut(s)	279, 394, 1288
FalI	AAGNNNNNCTT	2 cut(s)	2102, 2134
FaqI	GGGAC	2 cut(s)	383, 2194
FauI	CCCGC	1 cut(s)	1975
FauNDI	CATATG	2 cut(s)	670, 888
FbaI	TGATCA	1 cut(s)	715
FblI	GTMKAC	3 cut(s)	434, 681, 1572
Fnu4HI	GCNGC	8 cut(s)	115, 255, 1283, 1644, 1752, 1866, 1887, 1942
FokI	GGATG	4 cut(s)	2, 868, 1155, 1390
FriOI	GRGCYC	2 cut(s)	8, 1340
Fsp4HI	GCNGC	8 cut(s)	115, 255, 1283, 1644, 1752, 1866, 1887, 1942
FspAI	RTGCGCAY	1 cut(s)	358
FspBI	CTAG	3 cut(s)	930, 1302, 2027
FspI	TGCGCA	1 cut(s)	358
GlaI	GCGC	6 cut(s)	358, 385, 510, 697, 1252, 2067
GluI	GCNGC	8 cut(s)	115, 255, 1283, 1644, 1752, 1866, 1887, 1942
GsaI	CCCAGC	3 cut(s)	54, 840, 2181
HaeIII	GGCC	3 cut(s)	117, 264, 284
HapII	CCGG	3 cut(s)	703, 1626, 2290
HgaI	GACGC	1 cut(s)	375
HhaI	GCGC	6 cut(s)	359, 386, 511, 698, 1253, 2068
Hin6I	GCGC	6 cut(s)	357, 384, 509, 696, 1251, 2066
HinP1I	GCGC	6 cut(s)	357, 384, 509, 696, 1251, 2066
HincII	GTYRAC	1 cut(s)	1740
HindII	GTYRAC	1 cut(s)	1740
HindIII	AAGCTT	4 cut(s)	729, 867, 1578, 1686
HinfI	GANTC	5 cut(s)	179, 982, 1076, 1311, 2031
HpaII	CCGG	3 cut(s)	703, 1626, 2290
HphI	GGTGA	5 cut(s)	178, 590, 1247, 2120, 2288
Hpy166II	GTNNAC	9 cut(s)	154, 435, 682, 1007, 1348, 1573, 1740, 1765, 2221
Hpy188I	TCNGA	2 cut(s)	245, 884
Hpy188III	TCNNGA	4 cut(s)	43, 128, 863, 1187
Hpy8I	GTNNAC	9 cut(s)	154, 435, 682, 1007, 1348, 1573, 1740, 1765, 2221
Hpy99I	CGWCG	1 cut(s)	1726
HpyCH4III	ACNGT	6 cut(s)	275, 655, 829, 1157, 1813, 2135
HpyCH4IV	ACGT	2 cut(s)	1003, 1656
HpyF10VI	GCNNNNNNNGC	7 cut(s)	842, 1105, 1472, 1649, 1979, 2166, 2223
HpyF3I	CTNAG	4 cut(s)	1104, 1785, 1797, 1869
HpySE526I	ACGT	2 cut(s)	1003, 1656
HspAI	GCGC	6 cut(s)	357, 384, 509, 696, 1251, 2066
Ksp22I	TGATCA	1 cut(s)	715
Kzo9I	GATC	8 cut(s)	96, 322, 715, 1131, 1816, 1899, 2008, 2253
LmnI	GCTCC	8 cut(s)	11, 90, 130, 1290, 1627, 2041, 2091, 2104
Lsp1109I	GCAGC	6 cut(s)	1294, 1630, 1738, 1852, 1898, 1953
LweI	GCATC	9 cut(s)	236, 618, 786, 904, 1003, 1102, 1462, 1873, 2169
MaeI	CTAG	3 cut(s)	930, 1302, 2027
MaeII	ACGT	2 cut(s)	1003, 1656
MaeIII	GTNAC	3 cut(s)	1367, 2126, 2194
MalI	GATC	8 cut(s)	98, 324, 717, 1133, 1818, 1901, 2010, 2255
MboI	GATC	8 cut(s)	96, 322, 715, 1131, 1816, 1899, 2008, 2253
MboII	GAAGA	4 cut(s)	31, 184, 1514, 2111
MfeI	CAATTG	1 cut(s)	1057
MflI	RGATCY	1 cut(s)	96
MhlI	GDGCHC	5 cut(s)	8, 348, 1340, 1638, 2088
MluCI	AATT	9 cut(s)	555, 707, 1041, 1057, 1778, 1892, 2016, 2187, 2240
MlyI	GAGTC	2 cut(s)	173, 2040
MmeI	TCCRAC	3 cut(s)	132, 546, 1659
Mph1103I	ATGCAT	1 cut(s)	1713
MseI	TTAA	6 cut(s)	615, 789, 1040, 1209, 1593, 1956
MslI	CAYNNNNRTG	8 cut(s)	399, 990, 1067, 1376, 1439, 1461, 1467, 2133
MspA1I	CMGCKG	1 cut(s)	1944
MspI	CCGG	3 cut(s)	703, 1626, 2290
MspR9I	CCNGG	3 cut(s)	820, 1626, 1821
MunI	CAATTG	1 cut(s)	1057
Mva1269I	GAATGC	3 cut(s)	534, 847, 901
MvaI	CCWGG	2 cut(s)	820, 1821
MvnI	CGCG	3 cut(s)	386, 696, 1188
MwoI	GCNNNNNNNGC	7 cut(s)	842, 1105, 1472, 1649, 1979, 2166, 2223
NciI	CCSGG	1 cut(s)	1626
NcoI	CCATGG	2 cut(s)	394, 1288
NdeI	CATATG	2 cut(s)	670, 888
NdeII	GATC	8 cut(s)	96, 322, 715, 1131, 1816, 1899, 2008, 2253
NlaIV	GGNNCC	1 cut(s)	126
NmuCI	GTSAC	1 cut(s)	2126
NruI	TCGCGA	1 cut(s)	1188
NsbI	TGCGCA	1 cut(s)	358
NsiI	ATGCAT	1 cut(s)	1713
NspI	RCATGY	4 cut(s)	1051, 1223, 1354, 1554
OliI	CACNNNNGTG	2 cut(s)	1067, 2133
PaeR7I	CTCGAG	1 cut(s)	1065
PaqCI	CACCTGC	1 cut(s)	1075
PciI	ACATGT	2 cut(s)	1047, 1350
PctI	GAATGC	3 cut(s)	534, 847, 901
PfeI	GAWTC	3 cut(s)	982, 1076, 1311
PflMI	CCANNNNNTGG	1 cut(s)	1122
PfoI	TCCNGGA	1 cut(s)	818
PkrI	GCNGC	8 cut(s)	116, 256, 1284, 1645, 1753, 1867, 1888, 1943
PleI	GAGTC	2 cut(s)	173, 2039
PpsI	GAGTC	2 cut(s)	173, 2039
PscI	ACATGT	2 cut(s)	1047, 1350
Psp124BI	GAGCTC	2 cut(s)	8, 1340
Psp6I	CCWGG	2 cut(s)	818, 1819
PspFI	CCCAGC	3 cut(s)	50, 836, 2177
PspGI	CCWGG	2 cut(s)	818, 1819
PspN4I	GGNNCC	1 cut(s)	126
PspPI	GGNCC	1 cut(s)	116
PspXI	VCTCGAGB	1 cut(s)	1065
PstI	CTGCAG	2 cut(s)	1086, 1943
PsuI	RGATCY	1 cut(s)	96
PvuII	CAGCTG	1 cut(s)	1944
RruI	TCGCGA	1 cut(s)	1188
RsaI	GTAC	3 cut(s)	461, 1355, 2117
RsaNI	GTAC	3 cut(s)	460, 1354, 2116
RseI	CAYNNNNRTG	8 cut(s)	399, 990, 1067, 1376, 1439, 1461, 1467, 2133
SacI	GAGCTC	2 cut(s)	8, 1340
SaqAI	TTAA	6 cut(s)	615, 789, 1040, 1209, 1593, 1956
SatI	GCNGC	8 cut(s)	115, 255, 1283, 1644, 1752, 1866, 1887, 1942
Sau3AI	GATC	8 cut(s)	96, 322, 715, 1131, 1816, 1899, 2008, 2253
Sau96I	GGNCC	1 cut(s)	116
SbfI	CCTGCAGG	1 cut(s)	1086
ScaI	AGTACT	1 cut(s)	2117
SchI	GAGTC	2 cut(s)	173, 2040
ScrFI	CCNGG	3 cut(s)	820, 1626, 1821
SdaI	CCTGCAGG	1 cut(s)	1086
SduI	GDGCHC	5 cut(s)	8, 348, 1340, 1638, 2088
SfaNI	GCATC	9 cut(s)	236, 618, 786, 904, 1003, 1102, 1462, 1873, 2169
SfcI	CTRYAG	2 cut(s)	1082, 1939
Sfr274I	CTCGAG	1 cut(s)	1065
SlaI	CTCGAG	1 cut(s)	1065
SmiMI	CAYNNNNRTG	8 cut(s)	399, 990, 1067, 1376, 1439, 1461, 1467, 2133
SmlI	CTYRAG	2 cut(s)	373, 1065
SmoI	CTYRAG	2 cut(s)	373, 1065
Sse8387I	CCTGCAGG	1 cut(s)	1086
Sse9I	AATT	9 cut(s)	555, 707, 1041, 1057, 1778, 1892, 2016, 2187, 2240
SsiI	CCGC	6 cut(s)	9, 114, 255, 1365, 1548, 1982
SspI	AATATT	1 cut(s)	1806
SspMI	CTAG	3 cut(s)	930, 1302, 2027
SstI	GAGCTC	2 cut(s)	8, 1340
StyD4I	CCNGG	3 cut(s)	818, 1624, 1819
StyI	CCWWGG	3 cut(s)	279, 394, 1288
TaaI	ACNGT	6 cut(s)	275, 655, 829, 1157, 1813, 2135
TaiI	ACGT	2 cut(s)	1006, 1659
TaqI	TCGA	7 cut(s)	735, 862, 1066, 1130, 1666, 2011, 2191
TasI	AATT	9 cut(s)	555, 707, 1041, 1057, 1778, 1892, 2016, 2187, 2240
TatI	WGTACW	1 cut(s)	2115
TauI	GCSGC	2 cut(s)	117, 257
TfiI	GAWTC	3 cut(s)	982, 1076, 1311
Tru1I	TTAA	6 cut(s)	615, 789, 1040, 1209, 1593, 1956
Tru9I	TTAA	6 cut(s)	615, 789, 1040, 1209, 1593, 1956
TscAI	CASTG	4 cut(s)	30, 725, 1347, 2140
TseFI	GTSAC	1 cut(s)	2126
TseI	GCWGC	6 cut(s)	1282, 1643, 1751, 1865, 1886, 1941
Tsp45I	GTSAC	1 cut(s)	2126
TspDTI	ATGAA	9 cut(s)	467, 974, 1015, 1110, 1182, 1511, 1522, 1958, 2260
TspGWI	ACGGA	1 cut(s)	379
TspRI	CASTG	4 cut(s)	30, 725, 1347, 2140
Van91I	CCANNNNNTGG	1 cut(s)	1122
XapI	RAATTY	1 cut(s)	2240
XceI	RCATGY	4 cut(s)	1051, 1223, 1354, 1554
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1941, 2306
XhoI	CTCGAG	1 cut(s)	1065
XmiI	GTMKAC	3 cut(s)	434, 681, 1572
XspI	CTAG	3 cut(s)	930, 1302, 2027
ZrmI	AGTACT	1 cut(s)	2117
Zsp2I	ATGCAT	1 cut(s)	1713

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.