Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0004747

Physical Location & Seq

Genomic Coordinates Reverse (-)

8817222 .. 8819933

Gene Structure 2712 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00340380.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1386 bp

ATGCCTGTGAGGGATGTTCAGGCTTGGAATGCGATGATAGTTGGGTATATAGAAAATGGGTGTGTTGAACTTGCAGACGGGTTGTTTCAGAAGATGCCTGAGCGGGATTTTGAATCTTGGACGCAAATGGTAGATGGGTTGGTCGGGGCCCAAAGAGTTAATGATGCCTTGAAGCTATTCATGGAGATGCCAGAGAAATGTCCCAAAACATGGAACTCGATCCTTTTGAAATTAATAAGAAATGAGCTCACTAGAGAAGCTCATGCTTGTCTTGAGAAGACGCCTTACAGAGATGTCATATCATGGACAAATTTGATTGTTGGATATTTTGGAATCAGAGAGGTTGGCGTCGCAATTAAACTTTTTGAGTCCATGCCTACTCGAGATACAACTGCATGGAATGCCACGATATTTGGATTAAATGAAAACGATCGTGGTGAAGAAGGTTTGAAGCTTTTCATGAGAATGAAAGAATCAGGGCTATCACCAGACAAAGCTACATTTACTAGTGTTTTGACAATCTGTTCAGACTTACCAACCTTACACCTAGGTAGACAAACTCATGCACATATAGTAAAAGCAGGATTTGGTGACATTGTTTCAGTTTCTAATGCTATGGTTACCATGTATGCAAGATGTGGGAACATGGATTCTGCTTTACTGGAATTCTCGTCCATGCCTAGCCGTGATGTCATCTCTTGGAATTCTATAATATGTGGATATGCACATCATGGGAATGGTGATGTAGCTTTGGATATGTTCGAACGCATGAGATCAACAGATGTTCATCCAAATCCTATAACATTTGTCAATGTTCTATCCGCTTGTAGCCATGCAGGGTTGGTCAGCCAAGGTAAATACTACTTTGACATGATGAGATACACGTATTTTCTTCAACCTACAACTGAGCATTATACATGTATAGTTGATTTATTCGGTAGATTTGGGCTTATAGATGAGGCAATGAGTTTTCTAGATCAAATGAAAGCCGATGGATTTGAAATTCCTGCTAGTGTATGGGGGGCATTACTTGGAGCATGTAGAATCCACAAGAATATTGAGGTGGGTGAGATTGCTGGAGAGAGGGTTCTGGAAATAGAGCCTGGCAACTCCGGTGTATATTTGATTTTGGCAGAAATGTATTTGAGTAATGGAAGAAGAGAAGATGCTGGGCGGATTAAGGCAAGGATGAAAGAGAAAGGAGTCAAGAAGCAACCGGGGTGCAGTTGGATCGAGGTAAACAACATTGGGAATGTATTTCTTTCAGGGGACAAATCCCACCCCAAATTTAGGAAGATCTATTATGTGTTAGAATTACTGTATATAGAGATTGAGACTCAGTTTCCAAAACCTGCTGCATTGCTTCAACAAGTACAAGTTCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

461

Amino Acids

51.86

Weight (kDa)

5.83

Isoelectric Point (pI)

37.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	129 - 175	2.8e-08	PPR repeat family
PPR_2	PF13041	229 - 277	2e-10	PPR repeat family
PPR	PF01535	232 - 259	1e-06	PPR repeat
E_motif	PF20431	350 - 412	3.6e-19	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004747)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23330 AT3G23330
fragaria_vesca	FvH4_4g03820 FvH4_4g04640
malus_domestica	MD13G1215400.v1.1 MD15G1316700.v1.1 MD16G1226500.v1.1
prunus_persica	Prupe.1G040600_v2.0.a1 Prupe.1G048700_v2.0.a1
pyrus_communis	pycom13g18860
rosa_chinensis	RchiOBHm_Chr4g0393661 RchiOBHm_Chr4g0395421
rosa_laevigata	RLG00000009623 RLG00000009737
rosa_multiflora	Rmu_sc0004598.1_g000006 Rmu_sc0010124.1_g000005 Rmu_sc0010817.1_g000005
rosa_roxburghii	Rroxscaffold_5G00338850 Rroxscaffold_5G00340380
rosa_rugosa	Rorug03G0357200 Rorug03G0371800.1
rosa_samantha	Rh4AG048500 Rh4AG059800 Rh4BG044900 Rh4BG058400 Rh4CG052000 Rh4CG064100
rosa_wichuraiana	Rw0G003320 Rw0G022520 Rw4G003850 Rw4G004790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1360
AccB7I	CCANNNNNTGG	1 cut(s)	210
AccBSI	CCGCTC	1 cut(s)	103
AccI	GTMKAC	1 cut(s)	553
AciI	CCGC	3 cut(s)	103, 822, 1174
AclWI	GGATC	2 cut(s)	214, 1238
AcsI	RAATTY	5 cut(s)	310, 665, 703, 1002, 1286
AcyI	GRCGYC	2 cut(s)	281, 348
AfaI	GTAC	1 cut(s)	1374
AfiI	CCNNNNNNNGG	2 cut(s)	210, 1290
AflIII	ACRYGT	2 cut(s)	882, 917
AgsI	TTSAA	8 cut(s)	68, 113, 172, 229, 451, 896, 1001, 1367
AhlI	ACTAGT	1 cut(s)	506
AjnI	CCWGG	1 cut(s)	1102
AloI	GAACNNNNNNTCC	2 cut(s)	1071, 1103
AluBI	AGCT	6 cut(s)	175, 247, 260, 454, 497, 749
AluI	AGCT	6 cut(s)	175, 247, 260, 454, 497, 749
Alw21I	GWGCWC	1 cut(s)	249
Alw26I	GTCTC	1 cut(s)	1328
AlwI	GGATC	2 cut(s)	214, 1238
Ama87I	CYCGRG	1 cut(s)	381
AoxI	GGCC	1 cut(s)	147
ApaI	GGGCCC	1 cut(s)	151
ApeKI	GCWGC	1 cut(s)	1355
ApoI	RAATTY	5 cut(s)	310, 665, 703, 1002, 1286
AseI	ATTAAT	1 cut(s)	233
Asp700I	GAANNNNTTC	2 cut(s)	176, 455
AspA2I	CCTAGG	1 cut(s)	547
AspS9I	GGNCC	2 cut(s)	147, 148
AsuC2I	CCSGG	1 cut(s)	1218
AsuHPI	GGTGA	5 cut(s)	449, 477, 602, 752, 1079
AsuII	TTCGAA	1 cut(s)	762
AvaI	CYCGRG	1 cut(s)	381
AvrII	CCTAGG	1 cut(s)	547
BaeGI	GKGCMC	1 cut(s)	151
BanII	GRGCYC	2 cut(s)	151, 249
BarI	GAAGNNNNNNTAC	2 cut(s)	269, 301
BbsI	GAAGAC	1 cut(s)	284
Bbv12I	GWGCWC	1 cut(s)	249
BbvI	GCAGC	1 cut(s)	1342
BccI	CCATC	2 cut(s)	128, 986
BceAI	ACGGC	1 cut(s)	669
BcgI	CGANNNNNNTGC	2 cut(s)	1213, 1247
BciT130I	CCWGG	1 cut(s)	1104
BcnI	CCSGG	1 cut(s)	1218
BcoDI	GTCTC	1 cut(s)	1328
BcuI	ACTAGT	1 cut(s)	506
BfaI	CTAG	6 cut(s)	252, 507, 548, 681, 974, 1011
BfuAI	ACCTGC	1 cut(s)	1360
BglII	AGATCT	1 cut(s)	1296
BisI	GCNGC	1 cut(s)	1356
BlnI	CCTAGG	1 cut(s)	547
BlsI	GCNGC	1 cut(s)	1357
Bme1390I	CCNGG	2 cut(s)	1104, 1218
BmeT110I	CYCGRG	1 cut(s)	381
BmgT120I	GGNCC	2 cut(s)	147, 148
BmiI	GGNNCC	2 cut(s)	148, 149
BmrFI	CCNGG	2 cut(s)	1104, 1218
BmsI	GCATC	4 cut(s)	84, 154, 177, 1156
BpiI	GAAGAC	1 cut(s)	284
BpmI	CTGGAG	1 cut(s)	1098
Bpu10I	CCTNAGC	1 cut(s)	99
Bpu14I	TTCGAA	1 cut(s)	762
BpuEI	CTTGAG	1 cut(s)	293
BpuMI	CCSGG	1 cut(s)	1218
BsaAI	YACGTR	1 cut(s)	885
BsaBI	GATNNNNATC	1 cut(s)	786
BsaHI	GRCGYC	2 cut(s)	281, 348
BsaJI	CCNNGG	3 cut(s)	547, 850, 1217
BsaWI	WCCGGW	1 cut(s)	1112
BsaXI	ACNNNNNCTCC	2 cut(s)	1071, 1101
Bsc4I	CCNNNNNNNGG	2 cut(s)	210, 1290
Bse1I	ACTGG	1 cut(s)	666
Bse3DI	GCAATG	2 cut(s)	969, 1358
Bse8I	GATNNNNATC	1 cut(s)	786
BseBI	CCWGG	1 cut(s)	1104
BseDI	CCNNGG	3 cut(s)	547, 850, 1217
BseGI	GGATG	3 cut(s)	19, 787, 1194
BseJI	GATNNNNATC	1 cut(s)	786
BseLI	CCNNNNNNNGG	2 cut(s)	210, 1290
BseMI	GCAATG	2 cut(s)	969, 1358
BseMII	CTCAG	3 cut(s)	90, 897, 1352
BseNI	ACTGG	1 cut(s)	666
BseSI	GKGCMC	1 cut(s)	151
BseXI	GCAGC	1 cut(s)	1342
BseYI	CCCAGC	1 cut(s)	1169
BsgI	GTGCAG	1 cut(s)	1243
Bsh1285I	CGRYCG	1 cut(s)	433
BshFI	GGCC	1 cut(s)	149
BsiEI	CGRYCG	1 cut(s)	433
BsiHKAI	GWGCWC	1 cut(s)	249
BsiHKCI	CYCGRG	1 cut(s)	381
BsiSI	CCGG	2 cut(s)	1113, 1217
BslFI	GGGAC	2 cut(s)	186, 1283
BslI	CCNNNNNNNGG	2 cut(s)	210, 1290
BsmAI	GTCTC	1 cut(s)	1328
BsmFI	GGGAC	2 cut(s)	186, 1283
BsmI	GAATGC	2 cut(s)	34, 406
BsnI	GGCC	1 cut(s)	149
BsoBI	CYCGRG	1 cut(s)	381
Bsp119I	TTCGAA	1 cut(s)	762
Bsp120I	GGGCCC	1 cut(s)	147
Bsp1286I	GDGCHC	2 cut(s)	151, 249
Bsp143I	GATC	6 cut(s)	219, 430, 773, 976, 1230, 1296
BspACI	CCGC	3 cut(s)	103, 822, 1174
BspANI	GGCC	1 cut(s)	149
BspCNI	CTCAG	3 cut(s)	91, 898, 1351
BspHI	TCATGA	1 cut(s)	459
BspLI	GGNNCC	2 cut(s)	148, 149
BspMI	ACCTGC	1 cut(s)	1360
BspPI	GGATC	2 cut(s)	214, 1238
BspT104I	TTCGAA	1 cut(s)	762
BsrBI	CCGCTC	1 cut(s)	103
BsrDI	GCAATG	2 cut(s)	969, 1358
BsrI	ACTGG	1 cut(s)	666
BssECI	CCNNGG	3 cut(s)	547, 850, 1217
BssMI	GATC	6 cut(s)	219, 430, 773, 976, 1230, 1296
BssNI	GRCGYC	2 cut(s)	281, 348
BssT1I	CCWWGG	2 cut(s)	547, 850
Bst2UI	CCWGG	1 cut(s)	1104
Bst4CI	ACNGT	1 cut(s)	1320
Bst6I	CTCTTC	1 cut(s)	1153
BstACI	GRCGYC	2 cut(s)	281, 348
BstAPI	GCANNNNNTGC	1 cut(s)	401
BstBAI	YACGTR	1 cut(s)	885
BstBI	TTCGAA	1 cut(s)	762
BstDEI	CTNAG	3 cut(s)	99, 906, 1338
BstEII	GGTNACC	1 cut(s)	619
BstF5I	GGATG	3 cut(s)	19, 787, 1194
BstKTI	GATC	6 cut(s)	222, 433, 776, 979, 1233, 1299
BstMAI	GTCTC	1 cut(s)	1328
BstMBI	GATC	6 cut(s)	219, 430, 773, 976, 1230, 1296
BstMCI	CGRYCG	1 cut(s)	433
BstMWI	GCNNNNNNNGC	2 cut(s)	29, 401
BstNI	CCWGG	1 cut(s)	1104
BstNSI	RCATGY	2 cut(s)	921, 1041
BstPI	GGTNACC	1 cut(s)	619
BstSCI	CCNGG	2 cut(s)	1102, 1216
BstSLI	GKGCMC	1 cut(s)	151
BstV1I	GCAGC	1 cut(s)	1342
BstV2I	GAAGAC	1 cut(s)	284
BstX2I	RGATCY	1 cut(s)	1296
BstYI	RGATCY	1 cut(s)	1296
BsuRI	GGCC	1 cut(s)	149
BtgZI	GCGATG	1 cut(s)	47
BtsCI	GGATG	3 cut(s)	19, 787, 1194
BveI	ACCTGC	1 cut(s)	1360
CciI	TCATGA	1 cut(s)	459
Cfr13I	GGNCC	2 cut(s)	147, 148
CseI	GACGC	3 cut(s)	130, 289, 337
Csp6I	GTAC	1 cut(s)	1373
CspCI	CAANNNNNGTGG	2 cut(s)	394, 429
CviQI	GTAC	1 cut(s)	1373
DdeI	CTNAG	3 cut(s)	99, 906, 1338
DpnI	GATC	6 cut(s)	221, 432, 775, 978, 1232, 1298
DpnII	GATC	6 cut(s)	219, 430, 773, 976, 1230, 1296
Eam1104I	CTCTTC	1 cut(s)	1153
EarI	CTCTTC	1 cut(s)	1153
EciI	GGCGGA	1 cut(s)	1189
Ecl136II	GAGCTC	1 cut(s)	247
Eco130I	CCWWGG	2 cut(s)	547, 850
Eco24I	GRGCYC	2 cut(s)	151, 249
Eco53kI	GAGCTC	1 cut(s)	247
Eco88I	CYCGRG	1 cut(s)	381
Eco91I	GGTNACC	1 cut(s)	619
EcoICRI	GAGCTC	1 cut(s)	247
EcoO109I	RGGNCCY	1 cut(s)	147
EcoO65I	GGTNACC	1 cut(s)	619
EcoRI	GAATTC	2 cut(s)	665, 703
EcoRII	CCWGG	1 cut(s)	1102
EcoT14I	CCWWGG	2 cut(s)	547, 850
EcoT38I	GRGCYC	2 cut(s)	151, 249
ErhI	CCWWGG	2 cut(s)	547, 850
FaqI	GGGAC	2 cut(s)	186, 1283
FauI	CCCGC	1 cut(s)	96
FblI	GTMKAC	1 cut(s)	553
Fnu4HI	GCNGC	1 cut(s)	1356
FokI	GGATG	3 cut(s)	26, 774, 1201
FriOI	GRGCYC	2 cut(s)	151, 249
Fsp4HI	GCNGC	1 cut(s)	1356
FspBI	CTAG	6 cut(s)	252, 507, 548, 681, 974, 1011
GluI	GCNGC	1 cut(s)	1356
GsaI	CCCAGC	1 cut(s)	1173
GsuI	CTGGAG	1 cut(s)	1098
HaeIII	GGCC	1 cut(s)	149
HapII	CCGG	2 cut(s)	1113, 1217
HgaI	GACGC	3 cut(s)	130, 289, 337
Hin1I	GRCGYC	2 cut(s)	281, 348
HindIII	AAGCTT	1 cut(s)	452
HinfI	GANTC	8 cut(s)	113, 333, 368, 473, 650, 1044, 1203, 1336
HpaII	CCGG	2 cut(s)	1113, 1217
HphI	GGTGA	5 cut(s)	449, 477, 602, 752, 1079
Hpy166II	GTNNAC	2 cut(s)	554, 1240
Hpy188I	TCNGA	3 cut(s)	90, 338, 529
Hpy188III	TCNNGA	6 cut(s)	272, 383, 460, 974, 1091, 1207
Hpy8I	GTNNAC	2 cut(s)	554, 1240
Hpy99I	CGWCG	1 cut(s)	353
HpyAV	CCTTC	1 cut(s)	437
HpyCH4III	ACNGT	1 cut(s)	1320
HpyCH4IV	ACGT	1 cut(s)	884
HpyCH4V	TGCA	8 cut(s)	74, 395, 566, 632, 725, 836, 1224, 1358
HpyF10VI	GCNNNNNNNGC	2 cut(s)	29, 401
HpyF3I	CTNAG	3 cut(s)	99, 906, 1338
HpySE526I	ACGT	1 cut(s)	884
Hsp92I	GRCGYC	2 cut(s)	281, 348
Kzo9I	GATC	6 cut(s)	219, 430, 773, 976, 1230, 1296
LmnI	GCTCC	1 cut(s)	1034
Lsp1109I	GCAGC	1 cut(s)	1342
LweI	GCATC	4 cut(s)	84, 154, 177, 1156
MaeI	CTAG	6 cut(s)	252, 507, 548, 681, 974, 1011
MaeII	ACGT	1 cut(s)	884
MaeIII	GTNAC	2 cut(s)	590, 619
MalI	GATC	6 cut(s)	221, 432, 775, 978, 1232, 1298
MbiI	CCGCTC	1 cut(s)	103
MboI	GATC	6 cut(s)	219, 430, 773, 976, 1230, 1296
MboII	GAAGA	8 cut(s)	103, 289, 452, 884, 1167, 1170, 1175, 1306
MflI	RGATCY	1 cut(s)	1296
MhlI	GDGCHC	2 cut(s)	151, 249
MluCI	AATT	8 cut(s)	230, 310, 354, 665, 703, 1002, 1286, 1313
MlyI	GAGTC	3 cut(s)	377, 1212, 1330
MmeI	TCCRAC	2 cut(s)	301, 1208
MnlI	CCTC	6 cut(s)	3, 334, 952, 1054, 1077, 1228
MroXI	GAANNNNTTC	2 cut(s)	176, 455
MseI	TTAA	5 cut(s)	159, 233, 357, 419, 1179
MslI	CAYNNNNRTG	3 cut(s)	185, 464, 735
MspI	CCGG	2 cut(s)	1113, 1217
MspR9I	CCNGG	2 cut(s)	1104, 1218
Mva1269I	GAATGC	2 cut(s)	34, 406
MvaI	CCWGG	1 cut(s)	1104
MwoI	GCNNNNNNNGC	2 cut(s)	29, 401
NciI	CCSGG	1 cut(s)	1218
NdeII	GATC	6 cut(s)	219, 430, 773, 976, 1230, 1296
NlaIV	GGNNCC	2 cut(s)	148, 149
NmuCI	GTSAC	1 cut(s)	590
NspI	RCATGY	2 cut(s)	921, 1041
NspV	TTCGAA	1 cut(s)	762
PaeR7I	CTCGAG	1 cut(s)	381
PagI	TCATGA	1 cut(s)	459
PciI	ACATGT	1 cut(s)	917
PctI	GAATGC	2 cut(s)	34, 406
PdmI	GAANNNNTTC	2 cut(s)	176, 455
PfeI	GAWTC	5 cut(s)	113, 333, 473, 650, 1044
PflMI	CCANNNNNTGG	1 cut(s)	210
PkrI	GCNGC	1 cut(s)	1357
Ple19I	CGATCG	1 cut(s)	433
PleI	GAGTC	3 cut(s)	376, 1211, 1330
PpsI	GAGTC	3 cut(s)	376, 1211, 1330
Ppu21I	YACGTR	1 cut(s)	885
PscI	ACATGT	1 cut(s)	917
PshBI	ATTAAT	1 cut(s)	233
Psp124BI	GAGCTC	1 cut(s)	249
Psp6I	CCWGG	1 cut(s)	1102
PspEI	GGTNACC	1 cut(s)	619
PspFI	CCCAGC	1 cut(s)	1169
PspGI	CCWGG	1 cut(s)	1102
PspN4I	GGNNCC	2 cut(s)	148, 149
PspOMI	GGGCCC	1 cut(s)	147
PspPI	GGNCC	2 cut(s)	147, 148
PsuI	RGATCY	1 cut(s)	1296
PvuI	CGATCG	1 cut(s)	433
RsaI	GTAC	1 cut(s)	1374
RsaNI	GTAC	1 cut(s)	1373
RseI	CAYNNNNRTG	3 cut(s)	185, 464, 735
SacI	GAGCTC	1 cut(s)	249
SaqAI	TTAA	5 cut(s)	159, 233, 357, 419, 1179
SatI	GCNGC	1 cut(s)	1356
Sau3AI	GATC	6 cut(s)	219, 430, 773, 976, 1230, 1296
Sau96I	GGNCC	2 cut(s)	147, 148
SchI	GAGTC	3 cut(s)	377, 1212, 1330
ScrFI	CCNGG	2 cut(s)	1104, 1218
SduI	GDGCHC	2 cut(s)	151, 249
SfaNI	GCATC	4 cut(s)	84, 154, 177, 1156
Sfr274I	CTCGAG	1 cut(s)	381
SfuI	TTCGAA	1 cut(s)	762
SlaI	CTCGAG	1 cut(s)	381
SmiMI	CAYNNNNRTG	3 cut(s)	185, 464, 735
SmlI	CTYRAG	2 cut(s)	272, 381
SmoI	CTYRAG	2 cut(s)	272, 381
SpeI	ACTAGT	1 cut(s)	506
Sse9I	AATT	8 cut(s)	230, 310, 354, 665, 703, 1002, 1286, 1313
SsiI	CCGC	3 cut(s)	103, 822, 1174
SspI	AATATT	1 cut(s)	1057
SspMI	CTAG	6 cut(s)	252, 507, 548, 681, 974, 1011
SstI	GAGCTC	1 cut(s)	249
StyD4I	CCNGG	2 cut(s)	1102, 1216
StyI	CCWWGG	2 cut(s)	547, 850
TaaI	ACNGT	1 cut(s)	1320
TaiI	ACGT	1 cut(s)	887
TaqI	TCGA	4 cut(s)	218, 382, 762, 1233
TasI	AATT	8 cut(s)	230, 310, 354, 665, 703, 1002, 1286, 1313
TatI	WGTACW	1 cut(s)	1372
TfiI	GAWTC	5 cut(s)	113, 333, 473, 650, 1044
Tru1I	TTAA	5 cut(s)	159, 233, 357, 419, 1179
Tru9I	TTAA	5 cut(s)	159, 233, 357, 419, 1179
TseFI	GTSAC	1 cut(s)	590
TseI	GCWGC	1 cut(s)	1355
Tsp45I	GTSAC	1 cut(s)	590
TspDTI	ATGAA	7 cut(s)	169, 438, 448, 482, 776, 998, 1205
Van91I	CCANNNNNTGG	1 cut(s)	210
VspI	ATTAAT	1 cut(s)	233
XapI	RAATTY	5 cut(s)	310, 665, 703, 1002, 1286
XbaI	TCTAGA	1 cut(s)	973
XceI	RCATGY	2 cut(s)	921, 1041
XhoI	CTCGAG	1 cut(s)	381
XmaJI	CCTAGG	1 cut(s)	547
XmiI	GTMKAC	1 cut(s)	553
XmnI	GAANNNNTTC	2 cut(s)	176, 455
XspI	CTAG	6 cut(s)	252, 507, 548, 681, 974, 1011

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.