Rh4AG048500

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004747
Physical Location & Seq
Forward (+)
10174757 .. 10177126
2370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG048500.1

Sequence Viewer

Length: 978 bp
ATGACCTCCCTCCAAACCCTACTCAAAACCCTCTTCAAAAACCCCAGCACCATCAAAACCCCATCCCAAGCCAAACAGCTCCACGCCCAAATCCTCAAAACCAAACCCCCTTCACCTCCCGACCTCTCCTTCCTCGTCTCTCTTTACTCCAACCTCAACCTCCTACGCCACACCCTCACTCTCTTCAACACTCTCCACTTCCCTCCCTCCACCATTGCCTGGAAGTCCATCATCAGATGCTACGCTTCCCATGGCCTCTGGCACCATTCCTTGGCCTCATTTGTGGAGATGAGGACTTTTGGCGTGTGCCCAGATCGCCATGTTTTCCCTTCTGTGCTCAAATCGTGCGCGTCGGTTAGGGACTTGAGGTTAGGTGAGTCCGTTCATGGGTGCATTGTTCGGAGCGGTATGGAGTTTGATTTGTATACGTGTAATGCGCTCATGAATATGTACGCCAAGTTGGAGATTTTGGGTGCACACCAAGTGCTCGATGAAAGTCGCGACCGAAATCTAGAGGGTACTTTCGGGAGACATTTGGTTGGTTCTGTGGGACTTGACGGTGGTGGTGGTGAGAGCATTGATAGAGTGTTTAGGCAAAAGGAAGGCGAAAGAGTTATTGAAATGGACAGTGTGAGGAAGGTCTTCGACTTGATGCCAGAACGAGATATTGTGTCCTGGAATACAGTGATTGCAGGGGATGCGCAGAATGGGATGTATGAACAGGCTTTGGCGAGGGTCAGGGAGATGGGGAATGCCAACTTGAAGCCTGATTCTTTTACTTTGTCTAGCGTGCTTCCGGTTTTTGCAGAATATGTAGATGTTGTTAAGGGAAAGGAGATCCATGCGTATGCCATAAGACATGGATTTGATGCAGATGTGTTCATTGGCAGTAGCTTGATCGACATTTTCATCACATTTCGTATGAAGACATGGCAATCATACCAAATCAGTTCCACCATGTTCATAACTACCGCATAG

Protein Analysis

325

Amino Acids

36.46

Weight (kDa)

8.24

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 221 - 267 1.6e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 702
AccB1I GGYRCC 1 cut(s) 261
AccB7I CCANNNNNTGG 2 cut(s) 219, 271
AccBSI CCGCTC 1 cut(s) 405
AccI GTMKAC 1 cut(s) 425
AccII CGCG 2 cut(s) 350, 501
AciI CCGC 2 cut(s) 405, 972
AclWI GGATC 1 cut(s) 832
AdeI CACNNNGTG 1 cut(s) 484
AfaI GTAC 2 cut(s) 452, 520
AfiI CCNNNNNNNGG 3 cut(s) 219, 271, 387
AflIII ACRYGT 1 cut(s) 428
AgsI TTSAA 4 cut(s) 37, 187, 620, 763
AjnI CCWGG 2 cut(s) 218, 674
AluBI AGCT 2 cut(s) 79, 894
AluI AGCT 2 cut(s) 79, 894
Alw21I GWGCWC 3 cut(s) 339, 478, 489
Alw26I GTCTC 2 cut(s) 142, 523
Alw44I GTGCAC 1 cut(s) 474
AlwI GGATC 1 cut(s) 832
AoxI GGCC 2 cut(s) 253, 273
ApaLI GTGCAC 1 cut(s) 474
Asp700I GAANNNNTTC 1 cut(s) 641
AspLEI GCGC 3 cut(s) 350, 439, 703
AsuHPI GGTGA 3 cut(s) 105, 386, 581
BaeGI GKGCMC 2 cut(s) 311, 478
BanI GGYRCC 1 cut(s) 261
BbsI GAAGAC 2 cut(s) 634, 932
Bbv12I GWGCWC 3 cut(s) 339, 478, 489
BccI CCATC 4 cut(s) 59, 70, 236, 739
BciT130I CCWGG 2 cut(s) 220, 676
BcoDI GTCTC 2 cut(s) 142, 523
BfaI CTAG 2 cut(s) 512, 786
Bme1390I CCNGG 2 cut(s) 220, 676
BmiI GGNNCC 1 cut(s) 263
BmrFI CCNGG 2 cut(s) 220, 676
BmsI GCATC 4 cut(s) 227, 642, 688, 859
BpiI GAAGAC 2 cut(s) 634, 932
BpuEI CTTGAG 1 cut(s) 385
BsaAI YACGTR 1 cut(s) 429
BsaJI CCNNGG 2 cut(s) 250, 270
BsaWI WCCGGW 1 cut(s) 796
Bsc4I CCNNNNNNNGG 3 cut(s) 219, 271, 387
Bse3DI GCAATG 1 cut(s) 213
BseBI CCWGG 2 cut(s) 220, 676
BseDI CCNNGG 2 cut(s) 250, 270
BseGI GGATG 3 cut(s) 62, 703, 717
BseLI CCNNNNNNNGG 3 cut(s) 219, 271, 387
BseMI GCAATG 1 cut(s) 213
BseSI GKGCMC 2 cut(s) 311, 478
BseYI CCCAGC 1 cut(s) 44
Bsh1236I CGCG 2 cut(s) 350, 501
Bsh1285I CGRYCG 1 cut(s) 505
BshFI GGCC 2 cut(s) 255, 275
BshNI GGYRCC 1 cut(s) 261
BsiEI CGRYCG 1 cut(s) 505
BsiHKAI GWGCWC 3 cut(s) 339, 478, 489
BsiSI CCGG 1 cut(s) 797
BslFI GGGAC 2 cut(s) 374, 564
BslI CCNNNNNNNGG 3 cut(s) 219, 271, 387
BsmAI GTCTC 2 cut(s) 142, 523
BsmBI CGTCTC 1 cut(s) 142
BsmFI GGGAC 2 cut(s) 374, 564
BsmI GAATGC 1 cut(s) 757
BsnI GGCC 2 cut(s) 255, 275
Bsp1286I GDGCHC 4 cut(s) 311, 339, 478, 489
Bsp143I GATC 3 cut(s) 313, 837, 897
Bsp19I CCATGG 1 cut(s) 250
Bsp68I TCGCGA 1 cut(s) 501
BspACI CCGC 2 cut(s) 405, 972
BspANI GGCC 2 cut(s) 255, 275
BspFNI CGCG 2 cut(s) 350, 501
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 1 cut(s) 263
BspPI GGATC 1 cut(s) 832
BspT107I GGYRCC 1 cut(s) 261
BsrBI CCGCTC 1 cut(s) 405
BsrDI GCAATG 1 cut(s) 213
BssECI CCNNGG 2 cut(s) 250, 270
BssMI GATC 3 cut(s) 313, 837, 897
BssNAI GTATAC 1 cut(s) 426
BssT1I CCWWGG 2 cut(s) 250, 270
Bst1107I GTATAC 1 cut(s) 426
Bst2UI CCWGG 2 cut(s) 220, 676
Bst4CI ACNGT 3 cut(s) 560, 629, 685
Bst6I CTCTTC 2 cut(s) 38, 188
BstAPI GCANNNNNTGC 1 cut(s) 698
BstBAI YACGTR 1 cut(s) 429
BstC8I GCNNGC 1 cut(s) 791
BstDSI CCRYGG 1 cut(s) 250
BstF5I GGATG 3 cut(s) 62, 703, 717
BstFNI CGCG 2 cut(s) 350, 501
BstHHI GCGC 3 cut(s) 350, 439, 703
BstKTI GATC 3 cut(s) 316, 840, 900
BstMAI GTCTC 2 cut(s) 142, 523
BstMBI GATC 3 cut(s) 313, 837, 897
BstMCI CGRYCG 1 cut(s) 505
BstMWI GCNNNNNNNGC 2 cut(s) 315, 698
BstNI CCWGG 2 cut(s) 220, 676
BstSCI CCNGG 2 cut(s) 218, 674
BstSLI GKGCMC 2 cut(s) 311, 478
BstUI CGCG 2 cut(s) 350, 501
BstV2I GAAGAC 2 cut(s) 634, 932
BstX2I RGATCY 1 cut(s) 837
BstYI RGATCY 1 cut(s) 837
BstZ17I GTATAC 1 cut(s) 426
BsuRI GGCC 2 cut(s) 255, 275
BtgI CCRYGG 1 cut(s) 250
BtsCI GGATG 3 cut(s) 62, 703, 717
BtsIMutI CAGTG 2 cut(s) 634, 690
BtuMI TCGCGA 1 cut(s) 501
Cac8I GCNNGC 1 cut(s) 791
CciI TCATGA 1 cut(s) 441
CfoI GCGC 3 cut(s) 350, 439, 703
CseI GACGC 1 cut(s) 339
Csp6I GTAC 2 cut(s) 451, 519
CviAII CATG 8 cut(s) 251, 320, 386, 442, 842, 860, 930, 958
CviJI RGCY 7 cut(s) 71, 79, 255, 275, 725, 766, 894
CviKI_1 RGCY 7 cut(s) 71, 79, 255, 275, 725, 766, 894
CviQI GTAC 2 cut(s) 451, 519
DpnI GATC 3 cut(s) 315, 839, 899
DpnII GATC 3 cut(s) 313, 837, 897
DraIII CACNNNGTG 1 cut(s) 484
Eam1104I CTCTTC 2 cut(s) 38, 188
EarI CTCTTC 2 cut(s) 38, 188
Eco130I CCWWGG 2 cut(s) 250, 270
EcoRII CCWGG 2 cut(s) 218, 674
EcoT14I CCWWGG 2 cut(s) 250, 270
ErhI CCWWGG 2 cut(s) 250, 270
Esp3I CGTCTC 1 cut(s) 142
FaeI CATG 8 cut(s) 254, 323, 389, 445, 845, 863, 933, 961
FaqI GGGAC 2 cut(s) 374, 564
FatI CATG 8 cut(s) 250, 319, 385, 441, 841, 859, 929, 957
FblI GTMKAC 1 cut(s) 425
FokI GGATG 3 cut(s) 49, 710, 724
FspBI CTAG 2 cut(s) 512, 786
FspI TGCGCA 1 cut(s) 702
GlaI GCGC 3 cut(s) 349, 438, 702
GsaI CCCAGC 1 cut(s) 48
HaeIII GGCC 2 cut(s) 255, 275
HapII CCGG 1 cut(s) 797
HgaI GACGC 1 cut(s) 339
HhaI GCGC 3 cut(s) 350, 439, 703
Hin1II CATG 8 cut(s) 254, 323, 389, 445, 845, 863, 933, 961
Hin6I GCGC 3 cut(s) 348, 437, 701
HinP1I GCGC 3 cut(s) 348, 437, 701
HinfI GANTC 2 cut(s) 377, 770
HpaII CCGG 1 cut(s) 797
HphI GGTGA 3 cut(s) 105, 386, 581
Hpy166II GTNNAC 2 cut(s) 426, 476
Hpy188I TCNGA 2 cut(s) 236, 402
Hpy188III TCNNGA 5 cut(s) 119, 442, 500, 512, 526
Hpy8I GTNNAC 2 cut(s) 426, 476
Hpy99I CGWCG 1 cut(s) 355
HpyAV CCTTC 5 cut(s) 120, 139, 339, 596, 631
HpyCH4III ACNGT 3 cut(s) 560, 629, 685
HpyCH4IV ACGT 1 cut(s) 428
HpyCH4V TGCA 5 cut(s) 393, 476, 692, 806, 872
HpyF10VI GCNNNNNNNGC 2 cut(s) 315, 698
HpySE526I ACGT 1 cut(s) 428
Hsp92II CATG 8 cut(s) 254, 323, 389, 445, 845, 863, 933, 961
HspAI GCGC 3 cut(s) 348, 437, 701
Kzo9I GATC 3 cut(s) 313, 837, 897
LmnI GCTCC 2 cut(s) 84, 402
LweI GCATC 4 cut(s) 227, 642, 688, 859
MaeI CTAG 2 cut(s) 512, 786
MaeII ACGT 1 cut(s) 428
MalI GATC 3 cut(s) 315, 839, 899
MbiI CCGCTC 1 cut(s) 405
MboI GATC 3 cut(s) 313, 837, 897
MboII GAAGA 4 cut(s) 25, 175, 634, 937
MflI RGATCY 1 cut(s) 837
MhlI GDGCHC 4 cut(s) 311, 339, 478, 489
MlyI GAGTC 1 cut(s) 386
MmeI TCCRAC 2 cut(s) 174, 441
MroXI GAANNNNTTC 1 cut(s) 641
MseI TTAA 1 cut(s) 825
MslI CAYNNNNRTG 2 cut(s) 446, 846
MspI CCGG 1 cut(s) 797
MspR9I CCNGG 2 cut(s) 220, 676
Mva1269I GAATGC 1 cut(s) 757
MvaI CCWGG 2 cut(s) 220, 676
MvnI CGCG 2 cut(s) 350, 501
MwoI GCNNNNNNNGC 2 cut(s) 315, 698
NcoI CCATGG 1 cut(s) 250
NdeII GATC 3 cut(s) 313, 837, 897
NlaIII CATG 8 cut(s) 254, 323, 389, 445, 845, 863, 933, 961
NlaIV GGNNCC 1 cut(s) 263
NruI TCGCGA 1 cut(s) 501
NsbI TGCGCA 1 cut(s) 702
PagI TCATGA 1 cut(s) 441
PctI GAATGC 1 cut(s) 757
PdmI GAANNNNTTC 1 cut(s) 641
PfeI GAWTC 1 cut(s) 770
PflMI CCANNNNNTGG 2 cut(s) 219, 271
PfoI TCCNGGA 1 cut(s) 674
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
Ppu21I YACGTR 1 cut(s) 429
Psp6I CCWGG 2 cut(s) 218, 674
PspFI CCCAGC 1 cut(s) 44
PspGI CCWGG 2 cut(s) 218, 674
PspN4I GGNNCC 1 cut(s) 263
PsuI RGATCY 1 cut(s) 837
RruI TCGCGA 1 cut(s) 501
RsaI GTAC 2 cut(s) 452, 520
RsaNI GTAC 2 cut(s) 451, 519
RseI CAYNNNNRTG 2 cut(s) 446, 846
SaqAI TTAA 1 cut(s) 825
Sau3AI GATC 3 cut(s) 313, 837, 897
SchI GAGTC 1 cut(s) 386
ScrFI CCNGG 2 cut(s) 220, 676
SduI GDGCHC 4 cut(s) 311, 339, 478, 489
SfaNI GCATC 4 cut(s) 227, 642, 688, 859
SmiMI CAYNNNNRTG 2 cut(s) 446, 846
SmlI CTYRAG 1 cut(s) 364
SmoI CTYRAG 1 cut(s) 364
SsiI CCGC 2 cut(s) 405, 972
SspMI CTAG 2 cut(s) 512, 786
StyD4I CCNGG 2 cut(s) 218, 674
StyI CCWWGG 2 cut(s) 250, 270
TaaI ACNGT 3 cut(s) 560, 629, 685
TaiI ACGT 1 cut(s) 431
TaqI TCGA 3 cut(s) 489, 645, 900
TaqII GACCGA 1 cut(s) 519
TfiI GAWTC 1 cut(s) 770
Tru1I TTAA 1 cut(s) 825
Tru9I TTAA 1 cut(s) 825
TscAI CASTG 2 cut(s) 634, 690
TspDTI ATGAA 8 cut(s) 374, 458, 507, 732, 871, 898, 938, 952
TspGWI ACGGA 1 cut(s) 370
TspRI CASTG 2 cut(s) 634, 690
Van91I CCANNNNNTGG 2 cut(s) 219, 271
VneI GTGCAC 1 cut(s) 474
XbaI TCTAGA 1 cut(s) 511
XmiI GTMKAC 1 cut(s) 425
XmnI GAANNNNTTC 1 cut(s) 641
XspI CTAG 2 cut(s) 512, 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.