RLG00000009623

Pentatricopeptide repeat-containing protein At4g02750-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004747
Physical Location & Seq
Forward (+)
55509544 .. 55511586
2043 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009623

Sequence Viewer

Length: 1569 bp
ATGCTCTACTTCTCATGGTCCATTTCTAAGCTGAAACTTAGTCGACTCCTCTCTCTAAGCCTCACCAAATCCCTATGTACTGCGGCTGCAACCTCATTCTTTCAAACCCAGAATCTAAAGCCCCTCAACTCGAAAATCTCAACCTACATGCGCAATGGGTTTGTCGAAGAAGCCCAGAAGTTGTTCGACGAAATGCCTCAGAGAAACACCGTCACCTGGAACGCCATGATTCGTGGGTACTTTCTAAACGGGCAGTTCCATGATGCGGTCAGTTTGTTTAATCGGATGACTGAGCCGGATGTCTTCTCGTATAACACGGTGATTGCCGGGTTGATGCAATGTGGGGATGTCGAGGGTGCTAGGCGAGTCTTCGACGGGATGGTTTTCCGTGACATTGTGACTTGGAATTCAATGATTTCTGGGTATATTCGTAATGGCAGGATCGGTGGAGCAGTGCAAGTGTTTGATGGGATGCCGGTGAAGGATGTGGTTTCTTGGAATTTGGTTGTTGGGGGACTTGTGAAGTATGGGGAGATTGATTTGGCTGAGGAGTATTTTAAGAAGATGAGCATTCGAGATTGTGCTTCCTGGACTATAATGATTTCGGTGTTTGCTAATGCAGGACGGATTGTTGAAGCTCGAGAGCTCTTTGATGACATGCCTGTGAGGGATGTTCAGGCTTGGAATGCGATGATAGTTGGGTATATAGAAAATAGGTGTGTTGAACTTGCAGAGGGGTTGTTTCAGAAGATGCCTGAGCGGGATTTTGAATCTTGGACTCAAATGGTAGATGGGTTGGTCGAGGCCCAAAGAGTTAATGATGCCTTGAAGCTATTCATGGAGATGCCAGAGAAATGTCCCAAAACATGGAACTTGATCCTTTTGAAATTAATAAGAAATGAGCTCACTAGAGAAGCTCATGCTTGTCTTGAGAAGACGCCTTACAGAGATGTCATATCATGGACAAATTTGATTGTTGGATATTTTGGAATCAGAGAGGTTGGCGTCGCAATTAAACTTTTTGAGTCCATGCCTACTCGAGATACAACTGCATGGAATGCCACAATATTTGGATTAAATGAAAATGATTGTGGTGAAGAAGGTTTGAAGCTTTTCATGAGAATGAAAGAATCAGGGCTATCACCAGACAAAGCTACATTTACTAGTGTTTTGACAATCTGTTCAGACTTACCAACCTTACACCTAGGTAGACAAACTCATGCACATATAGTAAAAGCAGGATTTGGTGACATTGTTTCAGTTTCCAATGCTATGGTTACCATGTATGCAAGATGTGGGAACATGGATTCTGCTTTACTGGAATTCTCGTCCATGCCTAGCCGTGATGTCATCTCTTGGAATTCTATAATATGTGGATATGCACATCATGGGAATGGTGATGTAGCTTTGGAGATGTTCGAACGCATGAGATCAACAGATGTTCATCCAAATCCTATAACATTTGTCAATGTTCTATCCGCTTGTAGCCATGCAGGGTTGGTCAGCCAAGAGATTGAGACTCAGTTTCCAAAACCTGCTGCATTGCTTCAACAAGTACAGGTTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

523

Amino Acids

58.76

Weight (kDa)

5.71

Isoelectric Point (pI)

29.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 68 - 97 2.3e-07 PPR repeat family
PPR_1 PF12854 68 - 97 1.4e-06 PPR repeat
PPR PF01535 71 - 98 8.5e-08 PPR repeat
PPR_1 PF12854 98 - 128 2.9e-06 PPR repeat
PPR_2 PF13041 99 - 130 2.4e-06 PPR repeat family
PPR_1 PF12854 128 - 158 1.3e-06 PPR repeat
PPR_2 PF13041 130 - 159 1.6e-06 PPR repeat family
PPR PF01535 133 - 161 3.4e-07 PPR repeat
PPR PF01535 196 - 223 6.2e-06 PPR repeat
PPR_2 PF13041 348 - 394 2.5e-07 PPR repeat family
PPR_2 PF13041 448 - 496 2.5e-10 PPR repeat family
PPR_3 PF13812 449 - 495 2.9e-06 Pentatricopeptide repeat domain
PPR PF01535 451 - 478 6.7e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 152
Acc36I ACCTGC 1 cut(s) 1543
AccB7I CCANNNNNTGG 1 cut(s) 867
AccBSI CCGCTC 1 cut(s) 760
AccI GTMKAC 2 cut(s) 43, 1210
AciI CCGC 4 cut(s) 83, 266, 760, 1479
AclWI GGATC 2 cut(s) 449, 871
AcsI RAATTY 5 cut(s) 406, 499, 967, 1322, 1360
AcyI GRCGYC 2 cut(s) 938, 1005
AfaI GTAC 3 cut(s) 79, 239, 1557
AfiI CCNNNNNNNGG 3 cut(s) 216, 265, 867
AgsI TTSAA 9 cut(s) 104, 411, 635, 725, 770, 829, 886, 1108, 1550
AhlI ACTAGT 1 cut(s) 1163
AjnI CCWGG 2 cut(s) 215, 587
AluBI AGCT 9 cut(s) 31, 638, 646, 832, 904, 917, 1111, 1154, 1406
AluI AGCT 9 cut(s) 31, 638, 646, 832, 904, 917, 1111, 1154, 1406
Alw21I GWGCWC 2 cut(s) 648, 906
Alw26I GTCTC 1 cut(s) 1511
AlwI GGATC 2 cut(s) 449, 871
Ama87I CYCGRG 2 cut(s) 639, 1038
AoxI GGCC 1 cut(s) 804
ApeKI GCWGC 2 cut(s) 86, 1538
ApoI RAATTY 5 cut(s) 406, 499, 967, 1322, 1360
AseI ATTAAT 1 cut(s) 890
Asp700I GAANNNNTTC 3 cut(s) 182, 833, 1112
AspA2I CCTAGG 1 cut(s) 1204
AspLEI GCGC 1 cut(s) 153
AspS9I GGNCC 2 cut(s) 18, 805
AsuC2I CCSGG 1 cut(s) 328
AsuHPI GGTGA 8 cut(s) 55, 205, 331, 490, 1106, 1134, 1259, 1409
AsuII TTCGAA 1 cut(s) 1419
AvaI CYCGRG 2 cut(s) 639, 1038
AvaII GGWCC 1 cut(s) 18
AvrII CCTAGG 1 cut(s) 1204
BanII GRGCYC 2 cut(s) 648, 906
BarI GAAGNNNNNNTAC 2 cut(s) 926, 958
BbsI GAAGAC 3 cut(s) 295, 361, 941
Bbv12I GWGCWC 2 cut(s) 648, 906
BbvCI CCTCAGC 1 cut(s) 546
BbvI GCAGC 2 cut(s) 73, 1525
BccI CCATC 3 cut(s) 373, 461, 785
BceAI ACGGC 1 cut(s) 1326
BciT130I CCWGG 2 cut(s) 217, 589
BcnI CCSGG 1 cut(s) 328
BcoDI GTCTC 1 cut(s) 1511
BcuI ACTAGT 1 cut(s) 1163
BfaI CTAG 5 cut(s) 360, 909, 1164, 1205, 1338
BfuAI ACCTGC 1 cut(s) 1543
BisI GCNGC 3 cut(s) 84, 87, 1539
BlnI CCTAGG 1 cut(s) 1204
BlsI GCNGC 3 cut(s) 85, 88, 1540
Bme1390I CCNGG 3 cut(s) 217, 328, 589
Bme18I GGWCC 1 cut(s) 18
BmeT110I CYCGRG 2 cut(s) 639, 1038
BmgT120I GGNCC 2 cut(s) 18, 805
BmiI GGNNCC 1 cut(s) 1563
BmrFI CCNGG 3 cut(s) 217, 328, 589
BmsI GCATC 6 cut(s) 253, 324, 462, 741, 811, 834
BpiI GAAGAC 3 cut(s) 295, 361, 941
Bpu10I CCTNAGC 2 cut(s) 546, 756
Bpu14I TTCGAA 1 cut(s) 1419
BpuEI CTTGAG 1 cut(s) 950
BpuMI CCSGG 1 cut(s) 328
BsaBI GATNNNNATC 1 cut(s) 1443
BsaHI GRCGYC 2 cut(s) 938, 1005
BsaJI CCNNGG 1 cut(s) 1204
Bsc4I CCNNNNNNNGG 3 cut(s) 216, 265, 867
Bse118I RCCGGY 1 cut(s) 475
Bse1I ACTGG 1 cut(s) 1323
Bse3DI GCAATG 3 cut(s) 160, 344, 1541
Bse8I GATNNNNATC 1 cut(s) 1443
BseBI CCWGG 2 cut(s) 217, 589
BseDI CCNNGG 1 cut(s) 1204
BseGI GGATG 8 cut(s) 291, 304, 352, 384, 477, 490, 676, 1444
BseJI GATNNNNATC 1 cut(s) 1443
BseLI CCNNNNNNNGG 3 cut(s) 216, 265, 867
BseMI GCAATG 3 cut(s) 160, 344, 1541
BseMII CTCAG 5 cut(s) 212, 282, 537, 747, 1535
BseNI ACTGG 1 cut(s) 1323
BseRI GAGGAG 2 cut(s) 38, 563
BseXI GCAGC 2 cut(s) 73, 1525
BshFI GGCC 1 cut(s) 806
BsiHKAI GWGCWC 2 cut(s) 648, 906
BsiHKCI CYCGRG 2 cut(s) 639, 1038
BsiSI CCGG 3 cut(s) 296, 327, 476
BslFI GGGAC 2 cut(s) 528, 843
BslI CCNNNNNNNGG 3 cut(s) 216, 265, 867
BsmAI GTCTC 1 cut(s) 1511
BsmFI GGGAC 2 cut(s) 528, 843
BsmI GAATGC 3 cut(s) 570, 691, 1063
BsnI GGCC 1 cut(s) 806
BsoBI CYCGRG 2 cut(s) 639, 1038
Bsp119I TTCGAA 1 cut(s) 1419
Bsp1286I GDGCHC 2 cut(s) 648, 906
Bsp143I GATC 3 cut(s) 441, 876, 1430
BspACI CCGC 4 cut(s) 83, 266, 760, 1479
BspANI GGCC 1 cut(s) 806
BspCNI CTCAG 5 cut(s) 211, 283, 538, 748, 1534
BspHI TCATGA 1 cut(s) 1116
BspLI GGNNCC 1 cut(s) 1563
BspMI ACCTGC 1 cut(s) 1543
BspPI GGATC 2 cut(s) 449, 871
BspT104I TTCGAA 1 cut(s) 1419
BsrBI CCGCTC 1 cut(s) 760
BsrDI GCAATG 3 cut(s) 160, 344, 1541
BsrFI RCCGGY 1 cut(s) 475
BsrI ACTGG 1 cut(s) 1323
BssAI RCCGGY 1 cut(s) 475
BssECI CCNNGG 1 cut(s) 1204
BssMI GATC 3 cut(s) 441, 876, 1430
BssNI GRCGYC 2 cut(s) 938, 1005
BssT1I CCWWGG 1 cut(s) 1204
Bst2UI CCWGG 2 cut(s) 217, 589
Bst4CI ACNGT 2 cut(s) 211, 319
BstACI GRCGYC 2 cut(s) 938, 1005
BstAPI GCANNNNNTGC 1 cut(s) 1058
BstBI TTCGAA 1 cut(s) 1419
BstDEI CTNAG 8 cut(s) 27, 38, 56, 198, 291, 546, 756, 1521
BstEII GGTNACC 1 cut(s) 1276
BstF5I GGATG 8 cut(s) 291, 304, 352, 384, 477, 490, 676, 1444
BstHHI GCGC 1 cut(s) 153
BstKTI GATC 3 cut(s) 444, 879, 1433
BstMAI GTCTC 1 cut(s) 1511
BstMBI GATC 3 cut(s) 441, 876, 1430
BstMWI GCNNNNNNNGC 2 cut(s) 686, 1058
BstNI CCWGG 2 cut(s) 217, 589
BstNSI RCATGY 2 cut(s) 151, 661
BstPI GGTNACC 1 cut(s) 1276
BstSCI CCNGG 3 cut(s) 215, 326, 587
BstV1I GCAGC 2 cut(s) 73, 1525
BstV2I GAAGAC 3 cut(s) 295, 361, 941
BstXI CCANNNNNNTGG 1 cut(s) 1273
BsuRI GGCC 1 cut(s) 806
BtgZI GCGATG 1 cut(s) 704
BtsCI GGATG 8 cut(s) 291, 304, 352, 384, 477, 490, 676, 1444
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 1 cut(s) 459
BveI ACCTGC 1 cut(s) 1543
CciI TCATGA 1 cut(s) 1116
CfoI GCGC 1 cut(s) 153
Cfr10I RCCGGY 1 cut(s) 475
Cfr13I GGNCC 2 cut(s) 18, 805
CseI GACGC 2 cut(s) 946, 994
Csp6I GTAC 3 cut(s) 78, 238, 1556
CspCI CAANNNNNGTGG 2 cut(s) 1051, 1086
CviQI GTAC 3 cut(s) 78, 238, 1556
DdeI CTNAG 8 cut(s) 27, 38, 56, 198, 291, 546, 756, 1521
DpnI GATC 3 cut(s) 443, 878, 1432
DpnII GATC 3 cut(s) 441, 876, 1430
Ecl136II GAGCTC 2 cut(s) 646, 904
Eco130I CCWWGG 1 cut(s) 1204
Eco24I GRGCYC 2 cut(s) 648, 906
Eco47I GGWCC 1 cut(s) 18
Eco53kI GAGCTC 2 cut(s) 646, 904
Eco88I CYCGRG 2 cut(s) 639, 1038
Eco91I GGTNACC 1 cut(s) 1276
EcoICRI GAGCTC 2 cut(s) 646, 904
EcoO65I GGTNACC 1 cut(s) 1276
EcoRI GAATTC 3 cut(s) 406, 1322, 1360
EcoRII CCWGG 2 cut(s) 215, 587
EcoT14I CCWWGG 1 cut(s) 1204
EcoT38I GRGCYC 2 cut(s) 648, 906
ErhI CCWWGG 1 cut(s) 1204
FaqI GGGAC 2 cut(s) 528, 843
FauI CCCGC 1 cut(s) 753
FblI GTMKAC 2 cut(s) 43, 1210
Fnu4HI GCNGC 3 cut(s) 84, 87, 1539
FokI GGATG 8 cut(s) 298, 311, 359, 391, 484, 497, 683, 1431
FriOI GRGCYC 2 cut(s) 648, 906
Fsp4HI GCNGC 3 cut(s) 84, 87, 1539
FspBI CTAG 5 cut(s) 360, 909, 1164, 1205, 1338
FspI TGCGCA 1 cut(s) 152
GlaI GCGC 1 cut(s) 152
GluI GCNGC 3 cut(s) 84, 87, 1539
HaeIII GGCC 1 cut(s) 806
HapII CCGG 3 cut(s) 296, 327, 476
HgaI GACGC 2 cut(s) 946, 994
HhaI GCGC 1 cut(s) 153
Hin1I GRCGYC 2 cut(s) 938, 1005
Hin6I GCGC 1 cut(s) 151
HinP1I GCGC 1 cut(s) 151
HincII GTYRAC 1 cut(s) 44
HindII GTYRAC 1 cut(s) 44
HindIII AAGCTT 1 cut(s) 1109
HpaII CCGG 3 cut(s) 296, 327, 476
HphI GGTGA 8 cut(s) 55, 205, 331, 490, 1106, 1134, 1259, 1409
Hpy166II GTNNAC 2 cut(s) 44, 1211
Hpy188I TCNGA 5 cut(s) 201, 285, 747, 995, 1186
Hpy188III TCNNGA 5 cut(s) 575, 641, 929, 1040, 1117
Hpy8I GTNNAC 2 cut(s) 44, 1211
Hpy99I CGWCG 3 cut(s) 191, 377, 1010
HpyAV CCTTC 2 cut(s) 475, 1094
HpyCH4III ACNGT 2 cut(s) 211, 319
HpyF10VI GCNNNNNNNGC 2 cut(s) 686, 1058
HpyF3I CTNAG 8 cut(s) 27, 38, 56, 198, 291, 546, 756, 1521
Hsp92I GRCGYC 2 cut(s) 938, 1005
HspAI GCGC 1 cut(s) 151
Kzo9I GATC 3 cut(s) 441, 876, 1430
LmnI GCTCC 1 cut(s) 449
Lsp1109I GCAGC 2 cut(s) 73, 1525
LweI GCATC 6 cut(s) 253, 324, 462, 741, 811, 834
MaeI CTAG 5 cut(s) 360, 909, 1164, 1205, 1338
MaeIII GTNAC 5 cut(s) 211, 389, 397, 1247, 1276
MalI GATC 3 cut(s) 443, 878, 1432
MbiI CCGCTC 1 cut(s) 760
MboI GATC 3 cut(s) 441, 876, 1430
MboII GAAGA 7 cut(s) 179, 295, 361, 574, 760, 946, 1109
MhlI GDGCHC 2 cut(s) 648, 906
MluCI AATT 7 cut(s) 406, 499, 887, 967, 1011, 1322, 1360
MlyI GAGTC 5 cut(s) 39, 375, 772, 1034, 1513
MmeI TCCRAC 1 cut(s) 958
MroXI GAANNNNTTC 3 cut(s) 182, 833, 1112
MseI TTAA 6 cut(s) 279, 558, 816, 890, 1014, 1076
MslI CAYNNNNRTG 4 cut(s) 662, 842, 1121, 1392
MspI CCGG 3 cut(s) 296, 327, 476
MspR9I CCNGG 3 cut(s) 217, 328, 589
Mva1269I GAATGC 3 cut(s) 570, 691, 1063
MvaI CCWGG 2 cut(s) 217, 589
MwoI GCNNNNNNNGC 2 cut(s) 686, 1058
NciI CCSGG 1 cut(s) 328
NdeII GATC 3 cut(s) 441, 876, 1430
NlaIV GGNNCC 1 cut(s) 1563
NmuCI GTSAC 4 cut(s) 211, 389, 397, 1247
NsbI TGCGCA 1 cut(s) 152
NspI RCATGY 2 cut(s) 151, 661
NspV TTCGAA 1 cut(s) 1419
PaeR7I CTCGAG 2 cut(s) 639, 1038
PagI TCATGA 1 cut(s) 1116
PctI GAATGC 3 cut(s) 570, 691, 1063
PdmI GAANNNNTTC 3 cut(s) 182, 833, 1112
PfeI GAWTC 6 cut(s) 112, 229, 770, 990, 1130, 1307
PflMI CCANNNNNTGG 1 cut(s) 867
PfoI TCCNGGA 1 cut(s) 587
PkrI GCNGC 3 cut(s) 85, 88, 1540
PleI GAGTC 5 cut(s) 39, 374, 772, 1033, 1513
PpsI GAGTC 5 cut(s) 39, 374, 772, 1033, 1513
PshBI ATTAAT 1 cut(s) 890
Psp124BI GAGCTC 2 cut(s) 648, 906
Psp6I CCWGG 2 cut(s) 215, 587
PspEI GGTNACC 1 cut(s) 1276
PspGI CCWGG 2 cut(s) 215, 587
PspN4I GGNNCC 1 cut(s) 1563
PspPI GGNCC 2 cut(s) 18, 805
RsaI GTAC 3 cut(s) 79, 239, 1557
RsaNI GTAC 3 cut(s) 78, 238, 1556
RseI CAYNNNNRTG 4 cut(s) 662, 842, 1121, 1392
SacI GAGCTC 2 cut(s) 648, 906
SalI GTCGAC 1 cut(s) 42
SaqAI TTAA 6 cut(s) 279, 558, 816, 890, 1014, 1076
SatI GCNGC 3 cut(s) 84, 87, 1539
Sau3AI GATC 3 cut(s) 441, 876, 1430
Sau96I GGNCC 2 cut(s) 18, 805
SchI GAGTC 5 cut(s) 39, 375, 772, 1034, 1513
ScrFI CCNGG 3 cut(s) 217, 328, 589
SduI GDGCHC 2 cut(s) 648, 906
SfaNI GCATC 6 cut(s) 253, 324, 462, 741, 811, 834
Sfr274I CTCGAG 2 cut(s) 639, 1038
SfuI TTCGAA 1 cut(s) 1419
SinI GGWCC 1 cut(s) 18
SlaI CTCGAG 2 cut(s) 639, 1038
SmiMI CAYNNNNRTG 4 cut(s) 662, 842, 1121, 1392
SmlI CTYRAG 3 cut(s) 639, 929, 1038
SmoI CTYRAG 3 cut(s) 639, 929, 1038
SpeI ACTAGT 1 cut(s) 1163
Sse9I AATT 7 cut(s) 406, 499, 887, 967, 1011, 1322, 1360
SsiI CCGC 4 cut(s) 83, 266, 760, 1479
SspI AATATT 1 cut(s) 1068
SspMI CTAG 5 cut(s) 360, 909, 1164, 1205, 1338
SstI GAGCTC 2 cut(s) 648, 906
StyD4I CCNGG 3 cut(s) 215, 326, 587
StyI CCWWGG 1 cut(s) 1204
TaaI ACNGT 2 cut(s) 211, 319
TasI AATT 7 cut(s) 406, 499, 887, 967, 1011, 1322, 1360
TatI WGTACW 2 cut(s) 77, 1555
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 6 cut(s) 112, 229, 770, 990, 1130, 1307
Tru1I TTAA 6 cut(s) 279, 558, 816, 890, 1014, 1076
Tru9I TTAA 6 cut(s) 279, 558, 816, 890, 1014, 1076
TscAI CASTG 1 cut(s) 459
TseFI GTSAC 4 cut(s) 211, 389, 397, 1247
TseI GCWGC 2 cut(s) 86, 1538
Tsp45I GTSAC 4 cut(s) 211, 389, 397, 1247
TspDTI ATGAA 5 cut(s) 826, 1095, 1105, 1139, 1433
TspGWI ACGGA 2 cut(s) 377, 640
TspRI CASTG 1 cut(s) 459
Van91I CCANNNNNTGG 1 cut(s) 867
VpaK11BI GGWCC 1 cut(s) 18
VspI ATTAAT 1 cut(s) 890
XapI RAATTY 5 cut(s) 406, 499, 967, 1322, 1360
XceI RCATGY 2 cut(s) 151, 661
XhoI CTCGAG 2 cut(s) 639, 1038
XmaJI CCTAGG 1 cut(s) 1204
XmiI GTMKAC 2 cut(s) 43, 1210
XmnI GAANNNNTTC 3 cut(s) 182, 833, 1112
XspI CTAG 5 cut(s) 360, 909, 1164, 1205, 1338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.