pycom13g24120

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0014830
Physical Location & Seq
Forward (+)
21357499 .. 21359361
1863 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1290 bp
ATGCACATTGCATCGAATCCGGTATTCCATGAAAGAACTAAACATATCGGGGTTGATTGCCACTATGTTCGCAATCAAGTTCAGTCGAAGGTGATTCAATTTTTAAGGGGATGGGTTGATGTACCGTGTAGAGTGGGTTGTTTTCTGCGTTCTTCTACATTGTTGTCGCCAATAGTGTTTCTTGTTGTCAATACCCTTAATTCAAGGGATGTGTATCTAGTGGTTGAGGCTTGTTCCTTCTCTTCAACAGACATATTTTGGTTAGGTCTTGTAATCTTTTTCCTTACAATGAATATACAACTTGAAAACTCCAATATCTTCTCACCATTTGGTACGAGCATTGAATTTAATCAACTTCAGTCCAATGGTATGGGAGATCCGAGCAAATTAGTGAGTCATAATTATCTCGTCATTCATATGATGTTAGTGACATTATTGAGATTTAGCCAATTGAGTTTAGTCAAGTTGGTTATTAGAGCAATATATTTTATCCTCTGCATCCAATATTCTATTGTTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCGAGGGTTTACTTGGCACACATTCAATGAAGTGTCAAAATAAAGTTTTAAAAAGGATCACATCATCACTTTCACAATCCTATGCCAATTGGCGTGCGTTGATTCCCTCGCCATTGATCCTGTACTTTCATTTTTTTTATTGCCTAATTAAGACATATATACCTACAAGATTAATAATCCAATTAGAAAAAATTAGGATCTACGTGAATTTACGAAATTGCCCTAGAGGCAAGGATTTTGACTTCCCATATCCTTGTAGTACTCTTTGGTACGAACGTATAGGCGAAATCCGTTTGCGAGTCCGGATTATAACGGTATACTTATGGACGTTTGAAATTGTGAAGCTTGCAGCCAATCAAAAAGAAAGAAGAAAAGAAAAGAAAAAAAAAAAAAAAAAAGGGAAGAGAGCTTCCCGTGCACCCATACCCGCACCCCCGCGAGTCCATCTTCCAAGGCCGATTCCGGCCAACTCCGGTGGAGTTTTTCGATGCCACCACCACCATCTTGAAGCTCTCATTCCCCTCTACAAAACCCACCCAAGAACCACCTTGATTAACCATGGTATGGGGCGGTTTGAGGCCACGAAAGTTGACGAAAATCAATGGTGCTCCGGCCACCCTTTTCGATTTTCCGGCAAATTGGCAAAGCAATGGCCGATGCCACCACTTGGGCTTTGTAGCCCATCATCCCAGGAGCAAAACCCAACCAACTTTGACCCCGTTGGACCACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8 Exo_endo_phos

Protein Analysis

430

Amino Acids

49.58

Weight (kDa)

10.06

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 866
AccB7I CCANNNNNTGG 2 cut(s) 1120, 1223
AccI GTMKAC 1 cut(s) 873
AccII CGCG 1 cut(s) 994
AccIII TCCGGA 1 cut(s) 858
AciI CCGC 3 cut(s) 984, 992, 1126
AclWI GGATC 4 cut(s) 371, 620, 667, 761
AcoI YGGCCR 3 cut(s) 1020, 1168, 1208
AcsI RAATTY 2 cut(s) 344, 763
AcuI CTGAAG 1 cut(s) 341
AfaI GTAC 5 cut(s) 123, 334, 680, 817, 827
AfiI CCNNNNNNNGG 2 cut(s) 1120, 1223
AgsI TTSAA 8 cut(s) 98, 204, 246, 305, 344, 582, 890, 1064
AjnI CCWGG 1 cut(s) 1245
AluBI AGCT 3 cut(s) 901, 965, 1067
AluI AGCT 3 cut(s) 901, 965, 1067
Alw21I GWGCWC 2 cut(s) 976, 1166
Alw44I GTGCAC 1 cut(s) 972
AlwI GGATC 4 cut(s) 371, 620, 667, 761
Aor13HI TCCGGA 1 cut(s) 858
AoxI GGCC 5 cut(s) 1010, 1020, 1134, 1168, 1208
ApaLI GTGCAC 1 cut(s) 972
ApeKI GCWGC 1 cut(s) 905
ApoI RAATTY 2 cut(s) 344, 763
AseI ATTAAT 1 cut(s) 728
AspS9I GGNCC 1 cut(s) 1280
AsuHPI GGTGA 2 cut(s) 103, 315
AvaII GGWCC 1 cut(s) 1280
BaeGI GKGCMC 1 cut(s) 976
BaeI ACNNNNGTAYC 2 cut(s) 817, 850
Bbv12I GWGCWC 2 cut(s) 976, 1166
BbvI GCAGC 1 cut(s) 917
BccI CCATC 4 cut(s) 105, 1008, 1065, 1246
BciT130I CCWGG 1 cut(s) 1247
BfaI CTAG 2 cut(s) 218, 780
BglI GCCNNNNNGGC 1 cut(s) 783
BisI GCNGC 1 cut(s) 906
BlsI GCNGC 1 cut(s) 907
BmcAI AGTACT 1 cut(s) 817
Bme1390I CCNGG 1 cut(s) 1247
Bme18I GGWCC 1 cut(s) 1280
BmgT120I GGNCC 1 cut(s) 1280
BmrFI CCNGG 1 cut(s) 1247
BmsI GCATC 4 cut(s) 20, 507, 1034, 1203
BsaAI YACGTR 1 cut(s) 760
BsaBI GATNNNNATC 1 cut(s) 213
BsaJI CCNNGG 3 cut(s) 1007, 1114, 1245
BsaWI WCCGGW 3 cut(s) 19, 858, 1028
Bsc4I CCNNNNNNNGG 2 cut(s) 1120, 1223
Bse3DI GCAATG 2 cut(s) 6, 1211
Bse8I GATNNNNATC 1 cut(s) 213
BseAI TCCGGA 1 cut(s) 858
BseBI CCWGG 1 cut(s) 1247
BseDI CCNNGG 3 cut(s) 1007, 1114, 1245
BseGI GGATG 4 cut(s) 116, 214, 498, 1241
BseJI GATNNNNATC 1 cut(s) 213
BseLI CCNNNNNNNGG 2 cut(s) 1120, 1223
BseMI GCAATG 2 cut(s) 6, 1211
BseSI GKGCMC 1 cut(s) 976
BseXI GCAGC 1 cut(s) 917
Bsh1236I CGCG 1 cut(s) 994
BshFI GGCC 5 cut(s) 1012, 1022, 1136, 1170, 1210
BsiHKAI GWGCWC 2 cut(s) 976, 1166
BsiSI CCGG 6 cut(s) 20, 859, 1019, 1029, 1167, 1188
BslI CCNNNNNNNGG 2 cut(s) 1120, 1223
BsnI GGCC 5 cut(s) 1012, 1022, 1136, 1170, 1210
Bsp1286I GDGCHC 2 cut(s) 976, 1166
Bsp13I TCCGGA 1 cut(s) 858
Bsp143I GATC 4 cut(s) 376, 612, 672, 753
Bsp19I CCATGG 1 cut(s) 1114
BspACI CCGC 3 cut(s) 984, 992, 1126
BspANI GGCC 5 cut(s) 1012, 1022, 1136, 1170, 1210
BspEI TCCGGA 1 cut(s) 858
BspFNI CGCG 1 cut(s) 994
BspPI GGATC 4 cut(s) 371, 620, 667, 761
BsrDI GCAATG 2 cut(s) 6, 1211
BssECI CCNNGG 3 cut(s) 1007, 1114, 1245
BssMI GATC 4 cut(s) 376, 612, 672, 753
BssNAI GTATAC 1 cut(s) 874
BssT1I CCWWGG 2 cut(s) 1007, 1114
Bst1107I GTATAC 1 cut(s) 874
Bst2UI CCWGG 1 cut(s) 1247
Bst4CI ACNGT 3 cut(s) 126, 871, 1287
Bst6I CTCTTC 2 cut(s) 247, 953
BstBAI YACGTR 1 cut(s) 760
BstC8I GCNNGC 2 cut(s) 651, 903
BstDSI CCRYGG 1 cut(s) 1114
BstF5I GGATG 4 cut(s) 116, 214, 498, 1241
BstFNI CGCG 1 cut(s) 994
BstKTI GATC 4 cut(s) 379, 615, 675, 756
BstMBI GATC 4 cut(s) 376, 612, 672, 753
BstMWI GCNNNNNNNGC 2 cut(s) 783, 971
BstNI CCWGG 1 cut(s) 1247
BstSCI CCNGG 1 cut(s) 1245
BstSLI GKGCMC 1 cut(s) 976
BstUI CGCG 1 cut(s) 994
BstV1I GCAGC 1 cut(s) 917
BstX2I RGATCY 2 cut(s) 376, 753
BstXI CCANNNNNNTGG 1 cut(s) 370
BstYI RGATCY 2 cut(s) 376, 753
BstZ17I GTATAC 1 cut(s) 874
BsuRI GGCC 5 cut(s) 1012, 1022, 1136, 1170, 1210
BtgI CCRYGG 1 cut(s) 1114
BtsCI GGATG 4 cut(s) 116, 214, 498, 1241
Cac8I GCNNGC 2 cut(s) 651, 903
Cfr13I GGNCC 1 cut(s) 1280
Csp6I GTAC 5 cut(s) 122, 333, 679, 816, 826
CspCI CAANNNNNGTGG 4 cut(s) 1012, 1047, 1126, 1161
CviAII CATG 2 cut(s) 29, 1115
CviQI GTAC 5 cut(s) 122, 333, 679, 816, 826
DpnI GATC 4 cut(s) 378, 614, 674, 755
DpnII GATC 4 cut(s) 376, 612, 672, 753
DraI TTTAAA 1 cut(s) 606
EaeI YGGCCR 3 cut(s) 1020, 1168, 1208
Eam1104I CTCTTC 2 cut(s) 247, 953
EarI CTCTTC 2 cut(s) 247, 953
Eco130I CCWWGG 2 cut(s) 1007, 1114
Eco47I GGWCC 1 cut(s) 1280
Eco57I CTGAAG 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 1245
EcoT14I CCWWGG 2 cut(s) 1007, 1114
ErhI CCWWGG 2 cut(s) 1007, 1114
FaeI CATG 2 cut(s) 32, 1118
FatI CATG 2 cut(s) 28, 1114
FauI CCCGC 2 cut(s) 991, 999
FauNDI CATATG 1 cut(s) 417
FblI GTMKAC 1 cut(s) 873
Fnu4HI GCNGC 1 cut(s) 906
FokI GGATG 4 cut(s) 123, 221, 485, 1228
Fsp4HI GCNGC 1 cut(s) 906
FspBI CTAG 2 cut(s) 218, 780
GluI GCNGC 1 cut(s) 906
HaeIII GGCC 5 cut(s) 1012, 1022, 1136, 1170, 1210
HapII CCGG 6 cut(s) 20, 859, 1019, 1029, 1167, 1188
Hin1II CATG 2 cut(s) 32, 1118
HincII GTYRAC 1 cut(s) 1147
HindII GTYRAC 1 cut(s) 1147
HindIII AAGCTT 1 cut(s) 899
HinfI GANTC 7 cut(s) 16, 94, 394, 658, 855, 996, 1015
HpaII CCGG 6 cut(s) 20, 859, 1019, 1029, 1167, 1188
HphI GGTGA 2 cut(s) 103, 315
Hpy166II GTNNAC 4 cut(s) 566, 874, 974, 1147
Hpy188I TCNGA 1 cut(s) 381
Hpy188III TCNNGA 2 cut(s) 859, 1061
Hpy8I GTNNAC 4 cut(s) 566, 874, 974, 1147
HpyAV CCTTC 2 cut(s) 82, 247
HpyCH4III ACNGT 3 cut(s) 126, 871, 1287
HpyCH4IV ACGT 3 cut(s) 759, 832, 884
HpyCH4V TGCA 5 cut(s) 4, 11, 498, 905, 974
HpyF10VI GCNNNNNNNGC 2 cut(s) 783, 971
HpySE526I ACGT 3 cut(s) 759, 832, 884
Hsp92II CATG 2 cut(s) 32, 1118
Kpn2I TCCGGA 1 cut(s) 858
Kzo9I GATC 4 cut(s) 376, 612, 672, 753
LmnI GCTCC 2 cut(s) 1169, 1249
LpnPI CCDG 9 cut(s) 33, 689, 872, 1032, 1042, 1180, 1201, 1232, 1259
Lsp1109I GCAGC 1 cut(s) 917
LweI GCATC 4 cut(s) 20, 507, 1034, 1203
MaeI CTAG 2 cut(s) 218, 780
MaeII ACGT 3 cut(s) 759, 832, 884
MaeIII GTNAC 1 cut(s) 427
MalI GATC 4 cut(s) 378, 614, 674, 755
MboI GATC 4 cut(s) 376, 612, 672, 753
MboII GAAGA 6 cut(s) 144, 234, 310, 936, 970, 995
MfeI CAATTG 2 cut(s) 449, 643
MflI RGATCY 2 cut(s) 376, 753
MhlI GDGCHC 2 cut(s) 976, 1166
MlyI GAGTC 3 cut(s) 403, 864, 1005
MmeI TCCRAC 1 cut(s) 1258
MnlI CCTC 7 cut(s) 220, 503, 553, 673, 776, 1088, 1126
MroI TCCGGA 1 cut(s) 858
MseI TTAA 7 cut(s) 104, 198, 348, 605, 705, 728, 1110
MslI CAYNNNNRTG 1 cut(s) 416
MspI CCGG 6 cut(s) 20, 859, 1019, 1029, 1167, 1188
MspR9I CCNGG 1 cut(s) 1247
MunI CAATTG 2 cut(s) 449, 643
MvaI CCWGG 1 cut(s) 1247
MvnI CGCG 1 cut(s) 994
MwoI GCNNNNNNNGC 2 cut(s) 783, 971
NcoI CCATGG 1 cut(s) 1114
NdeI CATATG 1 cut(s) 417
NdeII GATC 4 cut(s) 376, 612, 672, 753
NlaIII CATG 2 cut(s) 32, 1118
NmuCI GTSAC 1 cut(s) 427
PfeI GAWTC 4 cut(s) 16, 94, 658, 1015
PflMI CCANNNNNTGG 2 cut(s) 1120, 1223
PkrI GCNGC 1 cut(s) 907
PleI GAGTC 3 cut(s) 402, 863, 1004
PpsI GAGTC 3 cut(s) 402, 863, 1004
Ppu21I YACGTR 1 cut(s) 760
PshBI ATTAAT 1 cut(s) 728
PsiI TTATAA 1 cut(s) 866
Psp6I CCWGG 1 cut(s) 1245
PspGI CCWGG 1 cut(s) 1245
PspPI GGNCC 1 cut(s) 1280
PsuI RGATCY 2 cut(s) 376, 753
RsaI GTAC 5 cut(s) 123, 334, 680, 817, 827
RsaNI GTAC 5 cut(s) 122, 333, 679, 816, 826
RseI CAYNNNNRTG 1 cut(s) 416
SaqAI TTAA 7 cut(s) 104, 198, 348, 605, 705, 728, 1110
SatI GCNGC 1 cut(s) 906
Sau3AI GATC 4 cut(s) 376, 612, 672, 753
Sau96I GGNCC 1 cut(s) 1280
ScaI AGTACT 1 cut(s) 817
SchI GAGTC 3 cut(s) 403, 864, 1005
ScrFI CCNGG 1 cut(s) 1247
SduI GDGCHC 2 cut(s) 976, 1166
SfaNI GCATC 4 cut(s) 20, 507, 1034, 1203
SinI GGWCC 1 cut(s) 1280
SmiMI CAYNNNNRTG 1 cut(s) 416
SsiI CCGC 3 cut(s) 984, 992, 1126
SspI AATATT 1 cut(s) 506
SspMI CTAG 2 cut(s) 218, 780
StyD4I CCNGG 1 cut(s) 1245
StyI CCWWGG 2 cut(s) 1007, 1114
TaaI ACNGT 3 cut(s) 126, 871, 1287
TaiI ACGT 3 cut(s) 762, 835, 887
TaqI TCGA 4 cut(s) 14, 86, 1042, 1180
TatI WGTACW 2 cut(s) 678, 815
TfiI GAWTC 4 cut(s) 16, 94, 658, 1015
Tru1I TTAA 7 cut(s) 104, 198, 348, 605, 705, 728, 1110
Tru9I TTAA 7 cut(s) 104, 198, 348, 605, 705, 728, 1110
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 1 cut(s) 905
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 5 cut(s) 45, 305, 404, 599, 674
TspGWI ACGGA 1 cut(s) 836
Van91I CCANNNNNTGG 2 cut(s) 1120, 1223
VneI GTGCAC 1 cut(s) 972
VpaK11BI GGWCC 1 cut(s) 1280
VspI ATTAAT 1 cut(s) 728
XapI RAATTY 2 cut(s) 344, 763
XmiI GTMKAC 1 cut(s) 873
XspI CTAG 2 cut(s) 218, 780
ZrmI AGTACT 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.