pycom14g02800

Vacuolar protein sorting-associated protein 55 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010180
Physical Location & Seq
Reverse (-)
2133260 .. 2135226
1967 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 342 bp
ATGTTTTCAGCAAGCATCTTGTTACAAATCCTGGCTTGTGCATTATACAACAATTGGTGGCCGATGTTATCCGCTCTGATGTACGTGCTGGTACCCATGCCTTGCTTATTCTTTGGTGGCGGTTCTACTCAGGTTTTATTTGCGAGGGAAAGCGATGGGTGGATAAATGCTGCTAAATTTTTGACTGGTGCATCAACTGTTGGGAGCATAGCCATTCCCATCATTCTTAGGCACGCTCATATGATTGAGACACCTGCTATGTGGATTGAATTCTTTTCATTCTTCATATTTGTCTGTACTGTCATGTGTTTCCACCGGGCTAGTCTCGAAGACGAGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps55

Protein Analysis

114

Amino Acids

12.76

Weight (kDa)

5.38

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps55 PF04133 2 - 109 9.3e-31 Vacuolar protein sorting 55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 262
Acc36I ACCTGC 1 cut(s) 262
Acc65I GGTACC 1 cut(s) 91
AccB1I GGYRCC 1 cut(s) 91
AccBSI CCGCTC 1 cut(s) 74
AciI CCGC 2 cut(s) 72, 120
AcoI YGGCCR 1 cut(s) 59
AcsI RAATTY 2 cut(s) 176, 269
AfaI GTAC 3 cut(s) 83, 93, 298
AgsI TTSAA 1 cut(s) 269
AjnI CCWGG 1 cut(s) 30
Alw26I GTCTC 2 cut(s) 242, 329
AoxI GGCC 1 cut(s) 59
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 2 cut(s) 176, 269
Asp718I GGTACC 1 cut(s) 91
AsuC2I CCSGG 1 cut(s) 317
BanI GGYRCC 1 cut(s) 91
BbsI GAAGAC 1 cut(s) 336
BbvI GCAGC 1 cut(s) 157
BccI CCATC 2 cut(s) 149, 227
BciT130I CCWGG 1 cut(s) 32
BcnI CCSGG 1 cut(s) 317
BcoDI GTCTC 2 cut(s) 242, 329
BfaI CTAG 1 cut(s) 321
BfuAI ACCTGC 1 cut(s) 262
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
Bme1390I CCNGG 2 cut(s) 32, 317
BmiI GGNNCC 1 cut(s) 93
BmrFI CCNGG 2 cut(s) 32, 317
BmsI GCATC 2 cut(s) 24, 200
BpiI GAAGAC 1 cut(s) 336
BpuMI CCSGG 1 cut(s) 317
BsaAI YACGTR 1 cut(s) 85
Bse1I ACTGG 1 cut(s) 190
BseBI CCWGG 1 cut(s) 32
BseMII CTCAG 1 cut(s) 143
BseNI ACTGG 1 cut(s) 190
BseXI GCAGC 1 cut(s) 157
BshFI GGCC 1 cut(s) 61
BshNI GGYRCC 1 cut(s) 91
BsiSI CCGG 1 cut(s) 316
BsmAI GTCTC 2 cut(s) 242, 329
BsnI GGCC 1 cut(s) 61
BspACI CCGC 2 cut(s) 72, 120
BspANI GGCC 1 cut(s) 61
BspCNI CTCAG 1 cut(s) 142
BspLI GGNNCC 1 cut(s) 93
BspMI ACCTGC 1 cut(s) 262
BspT107I GGYRCC 1 cut(s) 91
BsrBI CCGCTC 1 cut(s) 74
BsrI ACTGG 1 cut(s) 190
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 2 cut(s) 199, 301
BstBAI YACGTR 1 cut(s) 85
BstC8I GCNNGC 2 cut(s) 13, 234
BstDEI CTNAG 2 cut(s) 129, 227
BstMAI GTCTC 2 cut(s) 242, 329
BstNI CCWGG 1 cut(s) 32
BstSCI CCNGG 2 cut(s) 30, 315
BstV1I GCAGC 1 cut(s) 157
BstV2I GAAGAC 1 cut(s) 336
BsuRI GGCC 1 cut(s) 61
BtgZI GCGATG 1 cut(s) 168
BveI ACCTGC 1 cut(s) 262
Cac8I GCNNGC 2 cut(s) 13, 234
Csp6I GTAC 3 cut(s) 82, 92, 297
CviAII CATG 2 cut(s) 97, 304
CviJI RGCY 4 cut(s) 35, 61, 212, 320
CviKI_1 RGCY 4 cut(s) 35, 61, 212, 320
CviQI GTAC 3 cut(s) 82, 92, 297
DdeI CTNAG 2 cut(s) 129, 227
EaeI YGGCCR 1 cut(s) 59
EcoRI GAATTC 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 30
FaeI CATG 2 cut(s) 100, 307
FaiI YATR 8 cut(s) 46, 98, 209, 240, 242, 260, 287, 305
FatI CATG 2 cut(s) 96, 303
FauNDI CATATG 1 cut(s) 240
Fnu4HI GCNGC 1 cut(s) 171
Fsp4HI GCNGC 1 cut(s) 171
FspBI CTAG 1 cut(s) 321
GluI GCNGC 1 cut(s) 171
HaeIII GGCC 1 cut(s) 61
HapII CCGG 1 cut(s) 316
Hin1II CATG 2 cut(s) 100, 307
HpaII CCGG 1 cut(s) 316
Hpy188I TCNGA 1 cut(s) 78
Hpy188III TCNNGA 1 cut(s) 326
HpyCH4III ACNGT 2 cut(s) 199, 301
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 2 cut(s) 41, 191
HpyF3I CTNAG 2 cut(s) 129, 227
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 2 cut(s) 100, 307
KpnI GGTACC 1 cut(s) 95
LmnI GCTCC 1 cut(s) 204
LpnPI CCDG 7 cut(s) 17, 44, 74, 116, 171, 267, 329
Lsp1109I GCAGC 1 cut(s) 157
LweI GCATC 2 cut(s) 24, 200
MaeI CTAG 1 cut(s) 321
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 21
MbiI CCGCTC 1 cut(s) 74
MboII GAAGA 2 cut(s) 274, 341
MfeI CAATTG 1 cut(s) 52
MluCI AATT 3 cut(s) 52, 176, 269
MnlI CCTC 1 cut(s) 138
MspI CCGG 1 cut(s) 316
MspR9I CCNGG 2 cut(s) 32, 317
MunI CAATTG 1 cut(s) 52
MvaI CCWGG 1 cut(s) 32
NciI CCSGG 1 cut(s) 317
NdeI CATATG 1 cut(s) 240
NlaIII CATG 2 cut(s) 100, 307
NlaIV GGNNCC 1 cut(s) 93
PaqCI CACCTGC 1 cut(s) 262
PkrI GCNGC 1 cut(s) 172
Ppu21I YACGTR 1 cut(s) 85
Psp6I CCWGG 1 cut(s) 30
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 1 cut(s) 93
RsaI GTAC 3 cut(s) 83, 93, 298
RsaNI GTAC 3 cut(s) 82, 92, 297
SatI GCNGC 1 cut(s) 171
ScrFI CCNGG 2 cut(s) 32, 317
SetI ASST 3 cut(s) 87, 135, 256
SfaNI GCATC 2 cut(s) 24, 200
Sse9I AATT 3 cut(s) 52, 176, 269
SsiI CCGC 2 cut(s) 72, 120
SspMI CTAG 1 cut(s) 321
StyD4I CCNGG 2 cut(s) 30, 315
TaaI ACNGT 2 cut(s) 199, 301
TaiI ACGT 1 cut(s) 87
TaqI TCGA 1 cut(s) 327
TasI AATT 3 cut(s) 52, 176, 269
TatI WGTACW 1 cut(s) 296
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 2 cut(s) 267, 274
XapI RAATTY 2 cut(s) 176, 269
XspI CTAG 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.