pycom14g03070

pentatricopeptide repeat-containing protein At1g31840

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006716
Physical Location & Seq
Forward (+)
2369814 .. 2370817
1004 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 786 bp
ATGGCCCTTGCAAAAAATGGGCTGATGGGTGATGCTCTTAGGTTCTTCTTTCAAGCTTTGTATAGGGGCATTAAGCTCAATGTCTATACTTTCAACATGTTAATAGATGGTTGCTGCAGATTGAAACGATTGAGGGATGCTGTGAAGGTGTACATCAAACTGGGAGTTTATAACATAGGACCAGATCTGGTGATGTATACCGTGCTTATTAAAGGTATTTCTGAACTAGGGAGATTAAGCGATGCGTTGGTGTTTTTCTTCCAATCAGTAAAGAAGGGTTTCCTACCCAATGTTGTAACGTATTGCATATTAATATGTTGTTCAAAGAAGTTGTGTAGACAACCTGATGTTGTTACATACAACACAATGATTTGTGGTTATTGCTCTCAGAGAAGGTTAGACGTGGCTCTTCAACTTTTTGAAGAGATGATACAGGGACAGTGTAAAATGGAAGATGCAATGTTGATGATCAATACAATGCTGGAAAAGGATCCTGAACCTAATGTGGTCACATATAGTTGTCTGATTGACGGTTGTTTCAAGTCTGAAAACATGAAAAGTGCCTTTGAACTACGGGGCCTGGTGCCCGATGTAATAGCGTACAGGATTCTGATTCGTGGTTACTGCAAGGTAGGAAGAACGGTAGAAGGCCTCGTGTTGTATGGGCGTATGTGCATAAGCGGGATCATGCCGGATGCTGTCATACAAAGGACGATTGCGGAGCATATCCTTGAAACTGATCGGCAAAAATATGAGAGTACTCCCAACCACATGATTTGCAGATAA

Protein Analysis

262

Amino Acids

29.74

Weight (kDa)

8.79

Isoelectric Point (pI)

25.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 2 - 40 5.9e-08 PPR repeat family
PPR_1 PF12854 22 - 50 9.1e-09 PPR repeat
PPR_2 PF13041 27 - 72 1.1e-08 PPR repeat family
PPR_2 PF13041 61 - 106 6.7e-09 PPR repeat family
PPR_1 PF12854 114 - 143 1.3e-13 PPR repeat
PPR_2 PF13041 115 - 149 2e-11 PPR repeat family
PPR PF01535 118 - 145 7.2e-09 PPR repeat
PPR_2 PF13041 147 - 181 2.4e-07 PPR repeat family
PPR_1 PF12854 166 - 191 5e-07 PPR repeat
PPR_1 PF12854 192 - 224 4.2e-07 PPR repeat
PPR_2 PF13041 196 - 234 2.2e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 171
AccB1I GGYRCC 1 cut(s) 583
AccI GTMKAC 2 cut(s) 197, 337
AciI CCGC 2 cut(s) 681, 719
AclWI GGATC 3 cut(s) 485, 498, 692
AfaI GTAC 3 cut(s) 152, 602, 760
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 9 cut(s) 53, 94, 124, 324, 413, 422, 541, 569, 734
AjiI CACGTC 1 cut(s) 403
AjnI CCWGG 1 cut(s) 579
AluBI AGCT 2 cut(s) 56, 76
AluI AGCT 2 cut(s) 56, 76
AlwI GGATC 3 cut(s) 485, 498, 692
AoxI GGCC 3 cut(s) 3, 577, 649
ApeKI GCWGC 1 cut(s) 114
AseI ATTAAT 1 cut(s) 311
Asp700I GAANNNNTTC 1 cut(s) 278
AspS9I GGNCC 3 cut(s) 4, 179, 577
AsuHPI GGTGA 2 cut(s) 41, 202
AvaII GGWCC 1 cut(s) 179
BaeGI GKGCMC 1 cut(s) 588
BamHI GGATCC 1 cut(s) 490
BanI GGYRCC 1 cut(s) 583
BarI GAAGNNNNNNTAC 2 cut(s) 414, 446
BauI CACGAG 1 cut(s) 653
BbvI GCAGC 1 cut(s) 101
BccI CCATC 2 cut(s) 19, 101
BciT130I CCWGG 1 cut(s) 581
BclI TGATCA 1 cut(s) 468
BfaI CTAG 1 cut(s) 227
BfmI CTRYAG 1 cut(s) 115
BglII AGATCT 1 cut(s) 184
BisI GCNGC 1 cut(s) 115
BlsI GCNGC 1 cut(s) 116
BmcAI AGTACT 1 cut(s) 760
Bme1390I CCNGG 1 cut(s) 581
Bme18I GGWCC 1 cut(s) 179
BmgBI CACGTC 1 cut(s) 403
BmgT120I GGNCC 3 cut(s) 4, 179, 577
BmiI GGNNCC 3 cut(s) 492, 578, 585
BmrFI CCNGG 1 cut(s) 581
BmrI ACTGGG 1 cut(s) 170
BmsI GCATC 5 cut(s) 22, 127, 232, 445, 685
BmuI ACTGGG 1 cut(s) 170
Bse1I ACTGG 1 cut(s) 165
Bse3DI GCAATG 1 cut(s) 465
BseBI CCWGG 1 cut(s) 581
BseGI GGATG 2 cut(s) 142, 700
BseMI GCAATG 1 cut(s) 465
BseMII CTCAG 1 cut(s) 401
BseNI ACTGG 1 cut(s) 165
BseSI GKGCMC 1 cut(s) 588
BseXI GCAGC 1 cut(s) 101
BshFI GGCC 3 cut(s) 5, 579, 651
BshNI GGYRCC 1 cut(s) 583
BsiSI CCGG 1 cut(s) 692
BslFI GGGAC 1 cut(s) 450
BsmFI GGGAC 1 cut(s) 450
BsnI GGCC 3 cut(s) 5, 579, 651
Bsp1286I GDGCHC 1 cut(s) 588
Bsp1407I TGTACA 1 cut(s) 150
Bsp143I GATC 5 cut(s) 184, 468, 490, 684, 739
BspACI CCGC 2 cut(s) 681, 719
BspANI GGCC 3 cut(s) 5, 579, 651
BspCNI CTCAG 1 cut(s) 400
BspLI GGNNCC 3 cut(s) 492, 578, 585
BspMAI CTGCAG 1 cut(s) 119
BspPI GGATC 3 cut(s) 485, 498, 692
BspQI GCTCTTC 1 cut(s) 414
BspT107I GGYRCC 1 cut(s) 583
BsrDI GCAATG 1 cut(s) 465
BsrGI TGTACA 1 cut(s) 150
BsrI ACTGG 1 cut(s) 165
BssMI GATC 5 cut(s) 184, 468, 490, 684, 739
BssNAI GTATAC 1 cut(s) 198
BssSI CACGAG 1 cut(s) 653
Bst1107I GTATAC 1 cut(s) 198
Bst2BI CACGAG 1 cut(s) 653
Bst2UI CCWGG 1 cut(s) 581
Bst4CI ACNGT 4 cut(s) 202, 441, 533, 643
Bst6I CTCTTC 2 cut(s) 414, 417
BstAUI TGTACA 1 cut(s) 150
BstDEI CTNAG 2 cut(s) 38, 387
BstF5I GGATG 2 cut(s) 142, 700
BstKTI GATC 5 cut(s) 187, 471, 493, 687, 742
BstMBI GATC 5 cut(s) 184, 468, 490, 684, 739
BstNI CCWGG 1 cut(s) 581
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 1 cut(s) 579
BstSFI CTRYAG 1 cut(s) 115
BstSLI GKGCMC 1 cut(s) 588
BstV1I GCAGC 1 cut(s) 101
BstX2I RGATCY 2 cut(s) 184, 490
BstYI RGATCY 2 cut(s) 184, 490
BstZ17I GTATAC 1 cut(s) 198
BsuRI GGCC 3 cut(s) 5, 579, 651
BtgZI GCGATG 1 cut(s) 255
BtrI CACGTC 1 cut(s) 403
BtsCI GGATG 2 cut(s) 142, 700
BtsIMutI CAGTG 1 cut(s) 446
Cfr13I GGNCC 3 cut(s) 4, 179, 577
Csp6I GTAC 3 cut(s) 151, 601, 759
CspCI CAANNNNNGTGG 1 cut(s) 758
CviAII CATG 4 cut(s) 97, 553, 688, 772
CviJI RGCY 7 cut(s) 5, 22, 56, 76, 407, 579, 651
CviKI_1 RGCY 7 cut(s) 5, 22, 56, 76, 407, 579, 651
CviQI GTAC 3 cut(s) 151, 601, 759
DdeI CTNAG 2 cut(s) 38, 387
DpnI GATC 5 cut(s) 186, 470, 492, 686, 741
DpnII GATC 5 cut(s) 184, 468, 490, 684, 739
Eam1104I CTCTTC 2 cut(s) 414, 417
EarI CTCTTC 2 cut(s) 414, 417
Eco147I AGGCCT 1 cut(s) 651
Eco47I GGWCC 1 cut(s) 179
EcoO109I RGGNCCY 1 cut(s) 577
EcoRII CCWGG 1 cut(s) 579
FaeI CATG 4 cut(s) 100, 556, 691, 775
FaqI GGGAC 1 cut(s) 450
FatI CATG 4 cut(s) 96, 552, 687, 771
FauI CCCGC 1 cut(s) 674
FbaI TGATCA 1 cut(s) 468
FblI GTMKAC 2 cut(s) 197, 337
Fnu4HI GCNGC 1 cut(s) 115
FokI GGATG 2 cut(s) 149, 707
Fsp4HI GCNGC 1 cut(s) 115
FspBI CTAG 1 cut(s) 227
GluI GCNGC 1 cut(s) 115
HaeIII GGCC 3 cut(s) 5, 579, 651
HapII CCGG 1 cut(s) 692
Hin1II CATG 4 cut(s) 100, 556, 691, 775
HindIII AAGCTT 1 cut(s) 54
HinfI GANTC 2 cut(s) 607, 613
HpaII CCGG 1 cut(s) 692
HphI GGTGA 2 cut(s) 41, 202
Hpy166II GTNNAC 3 cut(s) 151, 198, 338
Hpy188I TCNGA 5 cut(s) 223, 390, 525, 547, 612
Hpy188III TCNNGA 1 cut(s) 494
Hpy8I GTNNAC 3 cut(s) 151, 198, 338
HpyAV CCTTC 4 cut(s) 139, 268, 387, 641
HpyCH4III ACNGT 4 cut(s) 202, 441, 533, 643
HpyCH4IV ACGT 2 cut(s) 299, 402
HpyCH4V TGCA 7 cut(s) 11, 117, 306, 458, 627, 675, 780
HpyF3I CTNAG 2 cut(s) 38, 387
HpySE526I ACGT 2 cut(s) 299, 402
Hsp92II CATG 4 cut(s) 100, 556, 691, 775
Ksp22I TGATCA 1 cut(s) 468
Kzo9I GATC 5 cut(s) 184, 468, 490, 684, 739
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 1 cut(s) 721
Lsp1109I GCAGC 1 cut(s) 101
LweI GCATC 5 cut(s) 22, 127, 232, 445, 685
MaeI CTAG 1 cut(s) 227
MaeII ACGT 2 cut(s) 299, 402
MaeIII GTNAC 4 cut(s) 295, 352, 508, 620
MalI GATC 5 cut(s) 186, 470, 492, 686, 741
MboI GATC 5 cut(s) 184, 468, 490, 684, 739
MboII GAAGA 6 cut(s) 37, 250, 401, 434, 464, 648
MflI RGATCY 2 cut(s) 184, 490
MhlI GDGCHC 1 cut(s) 588
MnlI CCTC 2 cut(s) 126, 662
MroXI GAANNNNTTC 1 cut(s) 278
MseI TTAA 5 cut(s) 72, 101, 210, 236, 311
MspI CCGG 1 cut(s) 692
MspR9I CCNGG 1 cut(s) 581
MvaI CCWGG 1 cut(s) 581
NdeII GATC 5 cut(s) 184, 468, 490, 684, 739
NlaIII CATG 4 cut(s) 100, 556, 691, 775
NlaIV GGNNCC 3 cut(s) 492, 578, 585
NmuCI GTSAC 1 cut(s) 508
NspI RCATGY 1 cut(s) 100
PceI AGGCCT 1 cut(s) 651
PciI ACATGT 1 cut(s) 96
PciSI GCTCTTC 1 cut(s) 414
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 2 cut(s) 607, 613
PkrI GCNGC 1 cut(s) 116
PscI ACATGT 1 cut(s) 96
PshBI ATTAAT 1 cut(s) 311
PsiI TTATAA 1 cut(s) 171
Psp6I CCWGG 1 cut(s) 579
PspGI CCWGG 1 cut(s) 579
PspN4I GGNNCC 3 cut(s) 492, 578, 585
PspPI GGNCC 3 cut(s) 4, 179, 577
PstI CTGCAG 1 cut(s) 119
PsuI RGATCY 2 cut(s) 184, 490
RsaI GTAC 3 cut(s) 152, 602, 760
RsaNI GTAC 3 cut(s) 151, 601, 759
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 5 cut(s) 72, 101, 210, 236, 311
SatI GCNGC 1 cut(s) 115
Sau3AI GATC 5 cut(s) 184, 468, 490, 684, 739
Sau96I GGNCC 3 cut(s) 4, 179, 577
ScaI AGTACT 1 cut(s) 760
ScrFI CCNGG 1 cut(s) 581
SduI GDGCHC 1 cut(s) 588
SfaNI GCATC 5 cut(s) 22, 127, 232, 445, 685
SfcI CTRYAG 1 cut(s) 115
SinI GGWCC 1 cut(s) 179
SseBI AGGCCT 1 cut(s) 651
SsiI CCGC 2 cut(s) 681, 719
SspMI CTAG 1 cut(s) 227
StuI AGGCCT 1 cut(s) 651
StyD4I CCNGG 1 cut(s) 579
TaaI ACNGT 4 cut(s) 202, 441, 533, 643
TaiI ACGT 2 cut(s) 302, 405
TatI WGTACW 2 cut(s) 150, 758
TfiI GAWTC 2 cut(s) 607, 613
Tru1I TTAA 5 cut(s) 72, 101, 210, 236, 311
Tru9I TTAA 5 cut(s) 72, 101, 210, 236, 311
TscAI CASTG 1 cut(s) 446
TseFI GTSAC 1 cut(s) 508
TseI GCWGC 1 cut(s) 114
Tsp45I GTSAC 1 cut(s) 508
TspDTI ATGAA 1 cut(s) 569
TspRI CASTG 1 cut(s) 446
VpaK11BI GGWCC 1 cut(s) 179
VspI ATTAAT 1 cut(s) 311
XceI RCATGY 1 cut(s) 100
XmiI GTMKAC 2 cut(s) 197, 337
XmnI GAANNNNTTC 1 cut(s) 278
XspI CTAG 1 cut(s) 227
ZrmI AGTACT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.