Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0012144

Physical Location & Seq

Genomic Coordinates Forward (+)

3319800 .. 3324104

Gene Structure 4305 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2358 bp

ATGTCCTTGGTTTTTTTTTTTTTTTTTTTAAATTTACCGTGTCGTCTTATCGTGTGTCGCCGTATTTATATCCTTTCACCAGACAGCCTAAAAGTCCCAAACCCCCCATTGAAACTACCACCCTCATCTCTCTCTCCTCCTAGGCTCCTCCCCCCAACTCTCTCTCTCTCTCCCCTAAACGTAACTTTCTCTCTCCTCTCCATAACCAGTATAGACTGTGAAAAAGTCAAAATGGGTCAGCTCCTCCCCAGCTCCACCACCCTCTTCGCTTCAAAGCCATCACCCACGTGCACCTTCCTCTCCCTCCTCATCATCGGCATTCTGGGTTTTTATACCCTGCATTTCCCCCACCCTCCACTCTCCTCCTCAAATTCCCATTCTGCCCTCCGATTCCGCCAGATTTTCCTCTCCCATGCCACCAACGCCACCCTCTCCTCCTACCTCCGCGCCCTTACCCTCCATCCCCACCTAGCCGGCACCCCGCCATCCTTGGACACCGCCAGCTTCGTCCAGGCCCATTTCCTGGGCCTGGGCCTTGTCACCCGCTCGGCCCACTATAACGCCCTCCTCTCCTACCCACTCCACTCCTCACTCTCTGCACATTTTATCAACGGCTCCCGCGTGGACATCCCCTTAACCGAGCCCGGCCTCAACAGTAATCGCGACGGCGTCGTTCCACCGTACCACGCGTACTCCCCCTCCGGGTCGGCGCACGCCAAGCTCGCCTTCGCCAACCACGGCACGGACGAGGACTATCGTGCGCTGGGGGAGCTCGGGGTGAACGTTAGCGGGTGCGTGGTGATTATTAGAAGAGGCGATGACGTGTCCAGAGGCGAAGTCGTTAGGAAGGCCGAGAAAAACGGTGCGCTGGCTGTGCTATTGTACACCGAGGGGGCCGGTGGTCAAGCGAATGGGGGGTTTAAGAAGGGTTTTGAGAGAGGGTTTCTTATGAACGGCGTGGGGGACCCACTGAGCCCCGGGTGGGCCGGGGTCGACGGAGCCGAGAGGCTGGACCTGGACGACCCTGAGGTACTGAAAAGGTTTCCGAAAATTCCATCCATGCCCCTGTCCGCGGAAGCTGCCGAAACCCTTCTGGGGTGGCTCGGCGGCTCTACTGTGCCGCCAGCGTGGCGGGAGTCGCTGCCCGCCGCAGTGCGCGGGGTGGGGCCCGGCCCGACAGTGGTGAATTTCACTTACAAGGGGGAGAAAAAGGTGGTGAAGATTCATAATGTTTTTGCTGTCATAAGGGGTTCACAAGAGCCCGACCGCTATGTGCTGCTTGGAAACCATAGAGATGCGTGGACGTATGGGGCTGTTGACCCCAATAGTGGAACTGCAGCATTACTTGACATTGCTCGTAGATATTCTCTTTTGATGCGTTCGGGTTGGAATCCTAGGAGGACGATTATTCTCTGTAGTTGGGACGCAGAAGAGTTTGGAATGGTAGGATCTACTGAGTGGGTTGAACAAAACATTGCCAATTTGGGCTCCAAAGCCGTTGCCTACCTTAATGTAGATTGTGCGGTTCAAGGGCCAGGATTCTTTGTCCGCTCGACTCCTCAGCTGGACAATCTCATTCTTGAGGTCACAAAGAAGGTTAAAGATCCAGACTCGGTGTGTGCCACTGTATATGAGAAATGGATGACCGCAAATAGAAGGGTTGATGTCCAAAGACTCAGTGCAGTGGATTCTGATTTTGCCCCATTTTTGCAACATGCAGGGATTCCTTCCATGGATATGTACTATGGAATTGATTTTTCTGTTTATCACACTGCATATGATGCTTATGAGTGGATGATAAACTTTGGAGATCCTTTCTTTCAGAGACATGTGGCTGTTGCTGGAATGTGGGGACTTCTTGCCCTTCACCTGGCTGATGATTCAGTTCTACCTTTCAATTTCCTCTCTTATGCGCATCAGTTAAAGCATTATGAAGATGTTTTGAGCAACTTGTTGGACGGGGGTGTATCTCTACATCCCCTCACAACAGCAATTCAGGAGTTTGCCTATGCCGCCAAAGAAGCTGAAGATGAAGCACAGGAGCTGAGAGAGCAAGAAAATACAAGTGAGCTTGTAATTTTGAAGAAGCGGGCACTGAATGATCGGCTGATGCTTGCTGAAAGAGGCTTCCTGGATTCAGATGGACTCGAAGGAAGACAGTGGTTCAAGCATCTTGTTTATGGGCCTCCTGGTGAGCATGGAAGCAAACTATCCTTCTTTCCCGGAGTAGGCGATGCAATGCTTCGAGCAAAGAGAATGAGTAGCAGAGAAGGGCAGGCCAAAATTCAGCACGAGATCTGGAGAGTTGCCAGAGCCATACAGAGGGCTGCAAATTCCCTGAGAGGAGACGTTTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004180 GO:0004181 GO:0005575 GO:0006508 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008233 GO:0008235 GO:0008237 GO:0008238 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009908 GO:0009909 GO:0010015 GO:0010051 GO:0010073 GO:0010074 GO:0010075 GO:0010080 GO:0010081 GO:0010082 GO:0010154 GO:0010305 GO:0016020 GO:0016021 GO:0016787 GO:0019538 GO:0019827 GO:0022414 GO:0022622 GO:0031224 GO:0032501 GO:0032502 GO:0040008 GO:0043170 GO:0044238 GO:0044425 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0061458 GO:0065007 GO:0070011 GO:0071704 GO:0080050 GO:0090567 GO:0098727 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000026 GO:2000034 GO:2000241 GO:2000280

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA Peptidase_M28 TFR_dimer

Protein Analysis

786

Amino Acids

86.2

Weight (kDa)

6.95

Isoelectric Point (pI)

35.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	243 - 303	6.6e-06	PA domain
Peptidase_M28	PF04389	410 - 597	2.7e-24	Peptidase family M28
TFR_dimer	PF04253	656 - 781	2.2e-30	Transferrin receptor-like dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012144)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54720
fragaria_vesca	FvH4_6g21160 FvH4_6g21160 FvH4_6g21160
malus_domestica	MD12G1047100.v1.1 MD14G1046000.v1.1
prunus_persica	Prupe.7G088100_v2.0.a1
pyrus_communis	pycom12g04070 pycom14g03860
rosa_chinensis	RchiOBHm_Chr3g0477621
rosa_laevigata	RLG00000023705
rosa_multiflora	Rmu_sc0000775.1_g000003
rosa_roxburghii	Rroxscaffold_6G00403800
rosa_rugosa	Rorug03G0162500
rosa_samantha	Rh3AG212400 Rh3BG246100 Rh3CG239700 Rh3DG239200
rosa_wichuraiana	Rw3G019230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1914
AccB1I	GGYRCC	1 cut(s)	476
AccB7I	CCANNNNNTGG	1 cut(s)	523
AccBSI	CCGCTC	2 cut(s)	546, 1551
AccI	GTMKAC	1 cut(s)	993
AccII	CGCG	6 cut(s)	447, 621, 663, 689, 1073, 1158
AclI	AACGTT	1 cut(s)	783
AclWI	GGATC	3 cut(s)	1456, 1598, 1805
AcsI	RAATTY	6 cut(s)	31, 370, 1050, 1186, 2283, 2332
AcuI	CTGAAG	1 cut(s)	2046
AcvI	CACGTG	1 cut(s)	288
AcyI	GRCGYC	1 cut(s)	669
AfaI	GTAC	5 cut(s)	683, 692, 884, 1032, 1742
AflIII	ACRYGT	3 cut(s)	687, 822, 1828
AgsI	TTSAA	7 cut(s)	112, 273, 1466, 1529, 1897, 2083, 2167
AjiI	CACGTC	1 cut(s)	823
AjnI	CCWGG	8 cut(s)	510, 522, 528, 1014, 1534, 1869, 2130, 2188
AjuI	GAANNNNNNNTTGG	2 cut(s)	710, 742
AleI	CACNNNNGTG	1 cut(s)	286
Alw21I	GWGCWC	2 cut(s)	293, 774
Alw26I	GTCTC	2 cut(s)	1819, 2340
Alw44I	GTGCAC	1 cut(s)	289
AlwI	GGATC	3 cut(s)	1456, 1598, 1805
AlwNI	CAGNNNCTG	1 cut(s)	2044
Ama87I	CYCGRG	2 cut(s)	773, 977
ApaI	GGGCCC	1 cut(s)	1170
ApaLI	GTGCAC	1 cut(s)	289
ApeKI	GCWGC	5 cut(s)	1079, 1141, 1276, 1337, 2327
ApoI	RAATTY	6 cut(s)	31, 370, 1050, 1186, 2283, 2332
Asp700I	GAANNNNTTC	1 cut(s)	1089
AspA2I	CCTAGG	2 cut(s)	140, 1394
AspLEI	GCGC	6 cut(s)	449, 712, 763, 868, 1158, 1915
AsuC2I	CCSGG	7 cut(s)	645, 703, 978, 979, 988, 1170, 2223
AsuHPI	GGTGA	9 cut(s)	69, 273, 532, 790, 811, 1195, 1228, 1859, 2204
AvaI	CYCGRG	2 cut(s)	773, 977
AvaII	GGWCC	2 cut(s)	964, 1012
AvrII	CCTAGG	2 cut(s)	140, 1394
AxyI	CCTNAGG	1 cut(s)	1026
BaeGI	GKGCMC	3 cut(s)	293, 1170, 2095
BanI	GGYRCC	1 cut(s)	476
BanII	GRGCYC	6 cut(s)	645, 774, 977, 1170, 1263, 1490
BauI	CACGAG	1 cut(s)	2291
BbrPI	CACGTG	1 cut(s)	288
BbsI	GAAGAC	1 cut(s)	2161
Bbv12I	GWGCWC	2 cut(s)	293, 774
BbvCI	CCTCAGC	1 cut(s)	1560
BbvI	GCAGC	5 cut(s)	1066, 1128, 1263, 1349, 2314
BccI	CCATC	5 cut(s)	286, 468, 493, 1063, 2135
BceAI	ACGGC	6 cut(s)	45, 628, 682, 754, 970, 1481
BciT130I	CCWGG	8 cut(s)	512, 524, 530, 1016, 1536, 1871, 2132, 2190
BcnI	CCSGG	7 cut(s)	645, 703, 978, 979, 988, 1170, 2223
BcoDI	GTCTC	2 cut(s)	1819, 2340
BfaI	CTAG	3 cut(s)	141, 470, 1395
BfmI	CTRYAG	2 cut(s)	1335, 1414
BglI	GCCNNNNNGGC	1 cut(s)	1129
BglII	AGATCT	1 cut(s)	2295
BisI	GCNGC	9 cut(s)	1080, 1108, 1121, 1142, 1149, 1277, 1338, 2013, 2328
BlnI	CCTAGG	2 cut(s)	140, 1394
BlsI	GCNGC	9 cut(s)	1081, 1109, 1122, 1143, 1150, 1278, 1339, 2014, 2329
Bme18I	GGWCC	2 cut(s)	964, 1012
BmeT110I	CYCGRG	2 cut(s)	773, 977
BmgBI	CACGTC	1 cut(s)	823
BmsI	GCATC	7 cut(s)	1285, 1365, 1771, 1924, 2100, 2179, 2224
BpiI	GAAGAC	1 cut(s)	2161
BpmI	CTGGAG	1 cut(s)	2320
Bpu10I	CCTNAGC	1 cut(s)	1560
BpuEI	CTTGAG	1 cut(s)	1601
BpuMI	CCSGG	7 cut(s)	645, 703, 978, 979, 988, 1170, 2223
BsaAI	YACGTR	1 cut(s)	288
BsaHI	GRCGYC	1 cut(s)	669
BsaXI	ACNNNNNCTCC	4 cut(s)	677, 683, 707, 713
Bse118I	RCCGGY	2 cut(s)	473, 896
Bse1I	ACTGG	1 cut(s)	207
Bse21I	CCTNAGG	1 cut(s)	1026
Bse3DI	GCAATG	3 cut(s)	1350, 1473, 2244
BseBI	CCWGG	8 cut(s)	512, 524, 530, 1016, 1536, 1871, 2132, 2190
BseGI	GGATG	7 cut(s)	460, 485, 627, 1055, 1647, 1800, 1975
BseMI	GCAATG	3 cut(s)	1350, 1473, 2244
BseMII	CTCAG	7 cut(s)	962, 1017, 1446, 1574, 1690, 2036, 2330
BseNI	ACTGG	1 cut(s)	207
BseSI	GKGCMC	3 cut(s)	293, 1170, 2095
BseXI	GCAGC	5 cut(s)	1066, 1128, 1263, 1349, 2314
BseYI	CCCAGC	2 cut(s)	248, 763
BsgI	GTGCAG	2 cut(s)	582, 1701
Bsh1236I	CGCG	6 cut(s)	447, 621, 663, 689, 1073, 1158
Bsh1285I	CGRYCG	1 cut(s)	1267
BshNI	GGYRCC	1 cut(s)	476
BsiEI	CGRYCG	1 cut(s)	1267
BsiHKAI	GWGCWC	2 cut(s)	293, 774
BsiHKCI	CYCGRG	2 cut(s)	773, 977
BsiSI	CCGG	8 cut(s)	474, 645, 702, 897, 978, 987, 1170, 2223
BslFI	GGGAC	4 cut(s)	80, 977, 1436, 1866
BsmAI	GTCTC	2 cut(s)	1819, 2340
BsmBI	CGTCTC	1 cut(s)	2340
BsmFI	GGGAC	4 cut(s)	80, 977, 1436, 1866
BsmI	GAATGC	1 cut(s)	318
BsoBI	CYCGRG	2 cut(s)	773, 977
Bsp120I	GGGCCC	1 cut(s)	1166
Bsp1286I	GDGCHC	8 cut(s)	293, 645, 774, 977, 1170, 1263, 1490, 2095
Bsp1407I	TGTACA	1 cut(s)	882
Bsp143I	GATC	5 cut(s)	1448, 1603, 1810, 2101, 2295
Bsp19I	CCATGG	1 cut(s)	1731
Bsp68I	TCGCGA	1 cut(s)	663
BspCNI	CTCAG	7 cut(s)	963, 1018, 1447, 1573, 1689, 2037, 2331
BspFNI	CGCG	6 cut(s)	447, 621, 663, 689, 1073, 1158
BspMAI	CTGCAG	1 cut(s)	1339
BspPI	GGATC	3 cut(s)	1456, 1598, 1805
BspT107I	GGYRCC	1 cut(s)	476
BsrBI	CCGCTC	2 cut(s)	546, 1551
BsrDI	GCAATG	3 cut(s)	1350, 1473, 2244
BsrFI	RCCGGY	2 cut(s)	473, 896
BsrGI	TGTACA	1 cut(s)	882
BsrI	ACTGG	1 cut(s)	207
BssAI	RCCGGY	2 cut(s)	473, 896
BssMI	GATC	5 cut(s)	1448, 1603, 1810, 2101, 2295
BssNI	GRCGYC	1 cut(s)	669
BssSI	CACGAG	1 cut(s)	2291
BssT1I	CCWWGG	5 cut(s)	6, 140, 489, 1394, 1731
Bst2BI	CACGAG	1 cut(s)	2291
Bst2UI	CCWGG	8 cut(s)	512, 524, 530, 1016, 1536, 1871, 2132, 2190
Bst4CI	ACNGT	9 cut(s)	39, 218, 656, 681, 863, 1117, 1180, 1627, 2160
Bst6I	CTCTTC	3 cut(s)	269, 805, 1425
BstACI	GRCGYC	1 cut(s)	669
BstAPI	GCANNNNNTGC	1 cut(s)	1781
BstAUI	TGTACA	1 cut(s)	882
BstBAI	YACGTR	1 cut(s)	288
BstDEI	CTNAG	7 cut(s)	971, 1026, 1455, 1560, 1676, 2045, 2339
BstDSI	CCRYGG	3 cut(s)	736, 1071, 1731
BstF5I	GGATG	7 cut(s)	460, 485, 627, 1055, 1647, 1800, 1975
BstFNI	CGCG	6 cut(s)	447, 621, 663, 689, 1073, 1158
BstHHI	GCGC	6 cut(s)	449, 712, 763, 868, 1158, 1915
BstKTI	GATC	5 cut(s)	1451, 1606, 1813, 2104, 2298
BstMAI	GTCTC	2 cut(s)	1819, 2340
BstMBI	GATC	5 cut(s)	1448, 1603, 1810, 2101, 2295
BstMCI	CGRYCG	1 cut(s)	1267
BstNI	CCWGG	8 cut(s)	512, 524, 530, 1016, 1536, 1871, 2132, 2190
BstNSI	RCATGY	2 cut(s)	1718, 1832
BstSFI	CTRYAG	2 cut(s)	1335, 1414
BstSLI	GKGCMC	3 cut(s)	293, 1170, 2095
BstUI	CGCG	6 cut(s)	447, 621, 663, 689, 1073, 1158
BstV1I	GCAGC	5 cut(s)	1066, 1128, 1263, 1349, 2314
BstV2I	GAAGAC	1 cut(s)	2161
BstX2I	RGATCY	4 cut(s)	1448, 1603, 1810, 2295
BstYI	RGATCY	4 cut(s)	1448, 1603, 1810, 2295
Bsu36I	CCTNAGG	1 cut(s)	1026
BtgI	CCRYGG	3 cut(s)	736, 1071, 1731
BtgZI	GCGATG	2 cut(s)	831, 2247
BtrI	CACGTC	1 cut(s)	823
BtsCI	GGATG	7 cut(s)	460, 485, 627, 1055, 1647, 1800, 1975
BtsI	GCAGTG	3 cut(s)	1158, 1689, 1770
BtsIMutI	CAGTG	9 cut(s)	968, 1158, 1185, 1623, 1684, 1689, 1770, 2093, 2165
BtuMI	TCGCGA	1 cut(s)	663
CaiI	CAGNNNCTG	1 cut(s)	2044
CfoI	GCGC	6 cut(s)	449, 712, 763, 868, 1158, 1915
Cfr10I	RCCGGY	2 cut(s)	473, 896
Cfr42I	CCGCGG	1 cut(s)	1074
Cfr9I	CCCGGG	1 cut(s)	977
CseI	GACGC	2 cut(s)	658, 1433
Csp6I	GTAC	5 cut(s)	682, 691, 883, 1031, 1741
CviAII	CATG	6 cut(s)	413, 1060, 1715, 1732, 1829, 2198
CviQI	GTAC	5 cut(s)	682, 691, 883, 1031, 1741
DdeI	CTNAG	7 cut(s)	971, 1026, 1455, 1560, 1676, 2045, 2339
DpnI	GATC	5 cut(s)	1450, 1605, 1812, 2103, 2297
DpnII	GATC	5 cut(s)	1448, 1603, 1810, 2101, 2295
DraI	TTTAAA	1 cut(s)	30
Eam1104I	CTCTTC	3 cut(s)	269, 805, 1425
EarI	CTCTTC	3 cut(s)	269, 805, 1425
EciI	GGCGGA	1 cut(s)	383
Ecl136II	GAGCTC	1 cut(s)	772
Eco130I	CCWWGG	5 cut(s)	6, 140, 489, 1394, 1731
Eco24I	GRGCYC	6 cut(s)	645, 774, 977, 1170, 1263, 1490
Eco47I	GGWCC	2 cut(s)	964, 1012
Eco53kI	GAGCTC	1 cut(s)	772
Eco57I	CTGAAG	1 cut(s)	2046
Eco72I	CACGTG	1 cut(s)	288
Eco81I	CCTNAGG	1 cut(s)	1026
Eco88I	CYCGRG	2 cut(s)	773, 977
EcoICRI	GAGCTC	1 cut(s)	772
EcoO109I	RGGNCCY	2 cut(s)	964, 1166
EcoRII	CCWGG	8 cut(s)	510, 522, 528, 1014, 1534, 1869, 2130, 2188
EcoT14I	CCWWGG	5 cut(s)	6, 140, 489, 1394, 1731
EcoT38I	GRGCYC	6 cut(s)	645, 774, 977, 1170, 1263, 1490
ErhI	CCWWGG	5 cut(s)	6, 140, 489, 1394, 1731
Esp3I	CGTCTC	1 cut(s)	2340
FaeI	CATG	6 cut(s)	416, 1063, 1718, 1735, 1832, 2201
FalI	AAGNNNNNCTT	2 cut(s)	710, 742
FaqI	GGGAC	4 cut(s)	80, 977, 1436, 1866
FatI	CATG	6 cut(s)	412, 1059, 1714, 1731, 1828, 2197
FauI	CCCGC	8 cut(s)	489, 551, 626, 782, 1125, 1151, 1153, 2082
FauNDI	CATATG	1 cut(s)	1777
FblI	GTMKAC	1 cut(s)	993
Fnu4HI	GCNGC	9 cut(s)	1080, 1108, 1121, 1142, 1149, 1277, 1338, 2013, 2328
FokI	GGATG	7 cut(s)	447, 472, 614, 1042, 1654, 1807, 1962
FriOI	GRGCYC	6 cut(s)	645, 774, 977, 1170, 1263, 1490
Fsp4HI	GCNGC	9 cut(s)	1080, 1108, 1121, 1142, 1149, 1277, 1338, 2013, 2328
FspAI	RTGCGCAY	1 cut(s)	1914
FspBI	CTAG	3 cut(s)	141, 470, 1395
FspI	TGCGCA	1 cut(s)	1914
GlaI	GCGC	6 cut(s)	448, 711, 762, 867, 1157, 1914
GluI	GCNGC	9 cut(s)	1080, 1108, 1121, 1142, 1149, 1277, 1338, 2013, 2328
GsaI	CCCAGC	2 cut(s)	252, 767
GsuI	CTGGAG	1 cut(s)	2320
HapII	CCGG	8 cut(s)	474, 645, 702, 897, 978, 987, 1170, 2223
HgaI	GACGC	2 cut(s)	658, 1433
HhaI	GCGC	6 cut(s)	449, 712, 763, 868, 1158, 1915
Hin1I	GRCGYC	1 cut(s)	669
Hin1II	CATG	6 cut(s)	416, 1063, 1718, 1735, 1832, 2201
Hin6I	GCGC	6 cut(s)	447, 710, 761, 866, 1156, 1913
HinP1I	GCGC	6 cut(s)	447, 710, 761, 866, 1156, 1913
HincII	GTYRAC	2 cut(s)	994, 1318
HindII	GTYRAC	2 cut(s)	994, 1318
HpaII	CCGG	8 cut(s)	474, 645, 702, 897, 978, 987, 1170, 2223
HphI	GGTGA	9 cut(s)	69, 273, 532, 790, 811, 1195, 1228, 1859, 2204
Hpy166II	GTNNAC	8 cut(s)	291, 625, 781, 885, 994, 1253, 1302, 1318
Hpy188I	TCNGA	6 cut(s)	389, 1047, 1693, 1824, 2140, 2357
Hpy188III	TCNNGA	6 cut(s)	662, 828, 1580, 1607, 1997, 2299
Hpy8I	GTNNAC	8 cut(s)	291, 625, 781, 885, 994, 1253, 1302, 1318
Hpy99I	CGWCG	3 cut(s)	668, 674, 998
HpyCH4III	ACNGT	9 cut(s)	39, 218, 656, 681, 863, 1117, 1180, 1627, 2160
HpyCH4IV	ACGT	6 cut(s)	180, 287, 783, 822, 1304, 2349
HpyF3I	CTNAG	7 cut(s)	971, 1026, 1455, 1560, 1676, 2045, 2339
HpySE526I	ACGT	6 cut(s)	180, 287, 783, 822, 1304, 2349
Hsp92I	GRCGYC	1 cut(s)	669
Hsp92II	CATG	6 cut(s)	416, 1063, 1718, 1735, 1832, 2201
HspAI	GCGC	6 cut(s)	447, 710, 761, 866, 1156, 1913
KflI	GGGWCCC	1 cut(s)	964
KroI	GCCGGC	1 cut(s)	473
KroNI	GCCGGC	1 cut(s)	475
KspI	CCGCGG	1 cut(s)	1074
Kzo9I	GATC	5 cut(s)	1448, 1603, 1810, 2101, 2295
LmnI	GCTCC	8 cut(s)	150, 246, 257, 620, 769, 998, 1493, 2041
Lsp1109I	GCAGC	5 cut(s)	1066, 1128, 1263, 1349, 2314
LweI	GCATC	7 cut(s)	1285, 1365, 1771, 1924, 2100, 2179, 2224
MaeI	CTAG	3 cut(s)	141, 470, 1395
MaeII	ACGT	6 cut(s)	180, 287, 783, 822, 1304, 2349
MaeIII	GTNAC	3 cut(s)	181, 538, 1585
MalI	GATC	5 cut(s)	1450, 1605, 1812, 2103, 2297
MbiI	CCGCTC	2 cut(s)	546, 1551
MboI	GATC	5 cut(s)	1448, 1603, 1810, 2101, 2295
MboII	GAAGA	8 cut(s)	256, 822, 1231, 1442, 1946, 2039, 2095, 2166
MflI	RGATCY	4 cut(s)	1448, 1603, 1810, 2295
MhlI	GDGCHC	8 cut(s)	293, 645, 774, 977, 1170, 1263, 1490, 2095
MluI	ACGCGT	1 cut(s)	687
MlyI	GAGTC	5 cut(s)	1145, 1549, 1604, 1668, 2139
MmeI	TCCRAC	2 cut(s)	1367, 1935
MroNI	GCCGGC	1 cut(s)	473
MroXI	GAANNNNTTC	1 cut(s)	1089
MseI	TTAA	6 cut(s)	29, 635, 921, 1509, 1599, 1922
MslI	CAYNNNNRTG	3 cut(s)	286, 1293, 1736
MspA1I	CMGCKG	2 cut(s)	1073, 1564
MspI	CCGG	8 cut(s)	474, 645, 702, 897, 978, 987, 1170, 2223
Mva1269I	GAATGC	1 cut(s)	318
MvaI	CCWGG	8 cut(s)	512, 524, 530, 1016, 1536, 1871, 2132, 2190
MvnI	CGCG	6 cut(s)	447, 621, 663, 689, 1073, 1158
NaeI	GCCGGC	1 cut(s)	475
NciI	CCSGG	7 cut(s)	645, 703, 978, 979, 988, 1170, 2223
NcoI	CCATGG	1 cut(s)	1731
NdeI	CATATG	1 cut(s)	1777
NdeII	GATC	5 cut(s)	1448, 1603, 1810, 2101, 2295
NgoMIV	GCCGGC	1 cut(s)	473
NlaIII	CATG	6 cut(s)	416, 1063, 1718, 1735, 1832, 2201
NmeAIII	GCCGAG	4 cut(s)	527, 877, 1027, 1083
NmuCI	GTSAC	2 cut(s)	538, 1585
NruI	TCGCGA	1 cut(s)	663
NsbI	TGCGCA	1 cut(s)	1914
NspI	RCATGY	2 cut(s)	1718, 1832
OliI	CACNNNNGTG	1 cut(s)	286
PciI	ACATGT	1 cut(s)	1828
PcsI	WCGNNNNNNNCGW	4 cut(s)	618, 744, 780, 999
PctI	GAATGC	1 cut(s)	318
PdiI	GCCGGC	1 cut(s)	475
PdmI	GAANNNNTTC	1 cut(s)	1089
PfeI	GAWTC	8 cut(s)	390, 1222, 1390, 1539, 1688, 1723, 1880, 2135
PflFI	GACNNNGTC	1 cut(s)	668
PflMI	CCANNNNNTGG	1 cut(s)	523
PfoI	TCCNGGA	2 cut(s)	2130, 2221
PkrI	GCNGC	9 cut(s)	1081, 1109, 1122, 1143, 1150, 1278, 1339, 2014, 2329
PleI	GAGTC	5 cut(s)	1144, 1549, 1604, 1668, 2139
PmaCI	CACGTG	1 cut(s)	288
PmlI	CACGTG	1 cut(s)	288
PpsI	GAGTC	5 cut(s)	1144, 1549, 1604, 1668, 2139
Ppu21I	YACGTR	1 cut(s)	288
PpuMI	RGGWCCY	1 cut(s)	964
PscI	ACATGT	1 cut(s)	1828
Psp124BI	GAGCTC	1 cut(s)	774
Psp1406I	AACGTT	1 cut(s)	783
Psp5II	RGGWCCY	1 cut(s)	964
Psp6I	CCWGG	8 cut(s)	510, 522, 528, 1014, 1534, 1869, 2130, 2188
PspCI	CACGTG	1 cut(s)	288
PspFI	CCCAGC	2 cut(s)	248, 763
PspGI	CCWGG	8 cut(s)	510, 522, 528, 1014, 1534, 1869, 2130, 2188
PspOMI	GGGCCC	1 cut(s)	1166
PspPPI	RGGWCCY	1 cut(s)	964
PstI	CTGCAG	1 cut(s)	1339
PstNI	CAGNNNCTG	1 cut(s)	2044
PsuI	RGATCY	4 cut(s)	1448, 1603, 1810, 2295
PsyI	GACNNNGTC	1 cut(s)	668
PvuII	CAGCTG	1 cut(s)	1564
RruI	TCGCGA	1 cut(s)	663
RsaI	GTAC	5 cut(s)	683, 692, 884, 1032, 1742
RsaNI	GTAC	5 cut(s)	682, 691, 883, 1031, 1741
RseI	CAYNNNNRTG	3 cut(s)	286, 1293, 1736
SacI	GAGCTC	1 cut(s)	774
SacII	CCGCGG	1 cut(s)	1074
SalI	GTCGAC	1 cut(s)	992
SaqAI	TTAA	6 cut(s)	29, 635, 921, 1509, 1599, 1922
SatI	GCNGC	9 cut(s)	1080, 1108, 1121, 1142, 1149, 1277, 1338, 2013, 2328
Sau3AI	GATC	5 cut(s)	1448, 1603, 1810, 2101, 2295
SchI	GAGTC	5 cut(s)	1145, 1549, 1604, 1668, 2139
SduI	GDGCHC	8 cut(s)	293, 645, 774, 977, 1170, 1263, 1490, 2095
SfaNI	GCATC	7 cut(s)	1285, 1365, 1771, 1924, 2100, 2179, 2224
SfcI	CTRYAG	2 cut(s)	1335, 1414
Sfr303I	CCGCGG	1 cut(s)	1074
SgrBI	CCGCGG	1 cut(s)	1074
SinI	GGWCC	2 cut(s)	964, 1012
SmaI	CCCGGG	1 cut(s)	979
SmiMI	CAYNNNNRTG	3 cut(s)	286, 1293, 1736
SmlI	CTYRAG	1 cut(s)	1580
SmoI	CTYRAG	1 cut(s)	1580
SspMI	CTAG	3 cut(s)	141, 470, 1395
SstI	GAGCTC	1 cut(s)	774
StyI	CCWWGG	5 cut(s)	6, 140, 489, 1394, 1731
TaaI	ACNGT	9 cut(s)	39, 218, 656, 681, 863, 1117, 1180, 1627, 2160
TaiI	ACGT	6 cut(s)	183, 290, 786, 825, 1307, 2352
TaqI	TCGA	4 cut(s)	993, 1553, 2148, 2245
TatI	WGTACW	2 cut(s)	882, 1740
TauI	GCSGC	4 cut(s)	1110, 1123, 1151, 2015
TfiI	GAWTC	8 cut(s)	390, 1222, 1390, 1539, 1688, 1723, 1880, 2135
Tru1I	TTAA	6 cut(s)	29, 635, 921, 1509, 1599, 1922
Tru9I	TTAA	6 cut(s)	29, 635, 921, 1509, 1599, 1922
TscAI	CASTG	9 cut(s)	975, 1158, 1185, 1630, 1684, 1689, 1777, 2100, 2165
TseFI	GTSAC	2 cut(s)	538, 1585
TseI	GCWGC	5 cut(s)	1079, 1141, 1276, 1337, 2327
Tsp45I	GTSAC	2 cut(s)	538, 1585
TspDTI	ATGAA	4 cut(s)	965, 1214, 1947, 2046
TspGWI	ACGGA	2 cut(s)	758, 1011
TspMI	CCCGGG	1 cut(s)	977
TspRI	CASTG	9 cut(s)	975, 1158, 1185, 1630, 1684, 1689, 1777, 2100, 2165
Tth111I	GACNNNGTC	1 cut(s)	668
Van91I	CCANNNNNTGG	1 cut(s)	523
VneI	GTGCAC	1 cut(s)	289
VpaK11BI	GGWCC	2 cut(s)	964, 1012
XapI	RAATTY	6 cut(s)	31, 370, 1050, 1186, 2283, 2332
XceI	RCATGY	2 cut(s)	1718, 1832
XmaI	CCCGGG	1 cut(s)	977
XmaJI	CCTAGG	2 cut(s)	140, 1394
XmiI	GTMKAC	1 cut(s)	993
XmnI	GAANNNNTTC	1 cut(s)	1089
XspI	CTAG	3 cut(s)	141, 470, 1395

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.