pycom14g04610

Homeobox-leucine zipper protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012671
Physical Location & Seq
Forward (+)
4295881 .. 4297775
1895 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1191 bp
ATGATTCCTGCCGCATCCGGTTCGTCCATAGGGCCCAGCCGCCCCCCGTACGCCACCGTAGTACTGCCTTCACCGCCAGAAACCGACTCTCCGACTTTCTCGGACTACCCTCGAGGACATAATTTTTTTATAATTCTAAATTATATGGTTGTCAATTGTCACTTTTTTAATTTACGTTTAAATTTTCATTTTTCTTTTCATCCGGTTAATTATCCGAGCACAACCCAAATTTTTACAACTTGTGGGATTAAATGGGTTGTGATTATTTTCTCATATTGGGTTGATTTTGTTACAGTGGCTGTGTCTGAACCGGCCGTGGTGGCCTCGGCTGAGGAGGCAGATGATACAGCGGCGCCGCTATCATCTCCGAACAGCACGGTTTCATCGTTTCAGATGGATTTTGGAATGAGAAGTGGAGGAAGATCGAGTAAAAGAGATTTGGAGGTTGATGCCGACAGAGCGAGTGATGACGAGGAGAACGGGTCGACTCGGAAGAAACTCAGGCGCTCTAAAGATCAATCGGCTTTTCTTGAGGAGAGCTTCAAAGAGCACAGCACTCTGAATCCTGTAAGTCAAGGGAACAAAGAATACTTAAGTTTTTTGTTTAGAACTTCAGAGAATTGCGAGTACTTAAAGAGATGCTGTGAAACACTGACAGAAGAGAATAGGAGGTTACGAAAAGAACTGCAAGAATTAAAAACTTTGAAGACCTCTCAGCCTTTCTACGTGCAGGCTGCAGCTGCCTGCCACCACACTCACCATGTGCCCCTCATGTGGGCGCGTGGTCACAACTGCCTCAGCCAACACCTCCACCACCACCAACAACAACCACATGAAGAACTGATTTGCTTTAACTTTTTTTCACAGTTTTGGGTGAAAAAAGCCAAAAACAAGAAGCTGCACAACCCAGCTTTGAAAAATCGGTTTTTTTTCATATCTGTTTTACATAAAAGTTTACCAAACACTATAATACTTTTTTTTTTTTTTCAAAAGAACTTTTACAAAAAAGTTTACCAAACACTCTGCTGCTTTATTTCACAGCCGTTTATTCTCATAGCACAGCAGAATCAGTTTTTTTTTAAAGCACAAAAATACCAAACCAGCTCAAAGACTGCTCCGAAAAAGCAGCCAAGGTTAAATCCATTTGCTGCTCAGGCCCACATGCAGCAGCATCAAGGTGCAGCTGCATGA

Protein Analysis

397

Amino Acids

45.51

Weight (kDa)

9.19

Isoelectric Point (pI)

51.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HALZ PF02183 208 - 235 7.4e-06 Homeobox associated leucine zipper
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 131
AbsI CCTCGAGG 1 cut(s) 111
AccB1I GGYRCC 1 cut(s) 352
AccI GTMKAC 1 cut(s) 485
AccII CGCG 1 cut(s) 781
AciI CCGC 5 cut(s) 12, 40, 74, 350, 356
AcoI YGGCCR 1 cut(s) 312
AcsI RAATTY 2 cut(s) 181, 228
AcuI CTGAAG 1 cut(s) 597
AcyI GRCGYC 1 cut(s) 353
AdeI CACNNNGTG 1 cut(s) 763
AfaI GTAC 3 cut(s) 50, 63, 629
AfiI CCNNNNNNNGG 2 cut(s) 774, 775
AflII CTTAAG 1 cut(s) 592
AgsI TTSAA 4 cut(s) 544, 706, 916, 989
AluBI AGCT 6 cut(s) 540, 740, 898, 911, 1104, 1184
AluI AGCT 6 cut(s) 540, 740, 898, 911, 1104, 1184
Alw21I GWGCWC 2 cut(s) 221, 552
AlwNI CAGNNNCTG 1 cut(s) 299
Ama87I CYCGRG 1 cut(s) 111
AoxI GGCC 4 cut(s) 32, 312, 321, 1155
ApaI GGGCCC 1 cut(s) 36
ApoI RAATTY 2 cut(s) 181, 228
AspLEI GCGC 3 cut(s) 355, 507, 781
AspS9I GGNCC 3 cut(s) 32, 33, 1156
AsuHPI GGTGA 3 cut(s) 63, 749, 886
AvaI CYCGRG 1 cut(s) 111
BaeGI GKGCMC 2 cut(s) 36, 768
BanI GGYRCC 1 cut(s) 352
BanII GRGCYC 1 cut(s) 36
BbsI GAAGAC 1 cut(s) 713
Bbv12I GWGCWC 2 cut(s) 221, 552
BbvCI CCTCAGC 2 cut(s) 330, 797
BccI CCATC 1 cut(s) 388
BceAI ACGGC 2 cut(s) 299, 1027
BcgI CGANNNNNNTGC 1 cut(s) 37
BfmI CTRYAG 1 cut(s) 735
BfoI RGCGCY 2 cut(s) 356, 508
BfrI CTTAAG 1 cut(s) 592
BglI GCCNNNNNGGC 1 cut(s) 320
BmcAI AGTACT 2 cut(s) 63, 629
BmeT110I CYCGRG 1 cut(s) 111
BmgT120I GGNCC 3 cut(s) 32, 33, 1156
BmiI GGNNCC 2 cut(s) 34, 354
BmsI GCATC 4 cut(s) 23, 439, 629, 1180
BpiI GAAGAC 1 cut(s) 713
Bpu10I CCTNAGC 3 cut(s) 330, 797, 1152
BpuEI CTTGAG 1 cut(s) 551
BsaAI YACGTR 1 cut(s) 727
BsaHI GRCGYC 1 cut(s) 353
BsaJI CCNNGG 3 cut(s) 315, 324, 1130
BsaWI WCCGGW 2 cut(s) 17, 202
BsaXI ACNNNNNCTCC 4 cut(s) 73, 103, 467, 497
Bsc4I CCNNNNNNNGG 2 cut(s) 774, 775
Bse118I RCCGGY 1 cut(s) 310
BseDI CCNNGG 3 cut(s) 315, 324, 1130
BseGI GGATG 2 cut(s) 14, 199
BseLI CCNNNNNNNGG 2 cut(s) 774, 775
BseMII CTCAG 5 cut(s) 321, 514, 728, 811, 1166
BseRI GAGGAG 3 cut(s) 347, 488, 548
BseSI GKGCMC 2 cut(s) 36, 768
BseX3I CGGCCG 1 cut(s) 312
BseYI CCCAGC 2 cut(s) 35, 907
BsgI GTGCAG 2 cut(s) 749, 884
Bsh1236I CGCG 1 cut(s) 781
Bsh1285I CGRYCG 1 cut(s) 315
BshFI GGCC 4 cut(s) 34, 314, 323, 1157
BshNI GGYRCC 1 cut(s) 352
BsiEI CGRYCG 1 cut(s) 315
BsiHKAI GWGCWC 2 cut(s) 221, 552
BsiHKCI CYCGRG 1 cut(s) 111
BsiSI CCGG 3 cut(s) 18, 203, 311
BsiWI CGTACG 1 cut(s) 48
BslI CCNNNNNNNGG 2 cut(s) 774, 775
BsnI GGCC 4 cut(s) 34, 314, 323, 1157
BsoBI CYCGRG 1 cut(s) 111
Bsp120I GGGCCC 1 cut(s) 32
Bsp1286I GDGCHC 4 cut(s) 36, 221, 552, 768
Bsp143I GATC 2 cut(s) 422, 514
BspACI CCGC 5 cut(s) 12, 40, 74, 350, 356
BspANI GGCC 4 cut(s) 34, 314, 323, 1157
BspCNI CTCAG 5 cut(s) 322, 513, 727, 810, 1165
BspFNI CGCG 1 cut(s) 781
BspLI GGNNCC 2 cut(s) 34, 354
BspMAI CTGCAG 1 cut(s) 739
BspT107I GGYRCC 1 cut(s) 352
BspTI CTTAAG 1 cut(s) 592
BsrFI RCCGGY 1 cut(s) 310
BssAI RCCGGY 1 cut(s) 310
BssECI CCNNGG 3 cut(s) 315, 324, 1130
BssMI GATC 2 cut(s) 422, 514
BssNI GRCGYC 1 cut(s) 353
BssT1I CCWWGG 1 cut(s) 1130
Bst4CI ACNGT 4 cut(s) 58, 295, 379, 867
Bst6I CTCTTC 1 cut(s) 654
BstACI GRCGYC 1 cut(s) 353
BstAFI CTTAAG 1 cut(s) 592
BstBAI YACGTR 1 cut(s) 727
BstC8I GCNNGC 2 cut(s) 732, 745
BstDEI CTNAG 5 cut(s) 330, 500, 714, 797, 1152
BstDSI CCRYGG 1 cut(s) 315
BstF5I GGATG 2 cut(s) 14, 199
BstFNI CGCG 1 cut(s) 781
BstH2I RGCGCY 2 cut(s) 356, 508
BstHHI GCGC 3 cut(s) 355, 507, 781
BstKTI GATC 2 cut(s) 425, 517
BstMBI GATC 2 cut(s) 422, 514
BstMCI CGRYCG 1 cut(s) 315
BstMWI GCNNNNNNNGC 6 cut(s) 73, 320, 335, 458, 740, 1154
BstNSI RCATGY 1 cut(s) 1165
BstSFI CTRYAG 1 cut(s) 735
BstSLI GKGCMC 2 cut(s) 36, 768
BstUI CGCG 1 cut(s) 781
BstV2I GAAGAC 1 cut(s) 713
BstZI CGGCCG 1 cut(s) 312
BsuRI GGCC 4 cut(s) 34, 314, 323, 1157
BtgI CCRYGG 1 cut(s) 315
BtsCI GGATG 2 cut(s) 14, 199
BtsIMutI CAGTG 2 cut(s) 300, 650
Cac8I GCNNGC 2 cut(s) 732, 745
CaiI CAGNNNCTG 1 cut(s) 299
CfoI GCGC 3 cut(s) 355, 507, 781
Cfr10I RCCGGY 1 cut(s) 310
Cfr13I GGNCC 3 cut(s) 32, 33, 1156
Csp6I GTAC 3 cut(s) 49, 62, 628
CviAII CATG 5 cut(s) 761, 772, 833, 1162, 1188
CviQI GTAC 3 cut(s) 49, 62, 628
DdeI CTNAG 5 cut(s) 330, 500, 714, 797, 1152
DinI GGCGCC 1 cut(s) 354
DpnI GATC 2 cut(s) 424, 516
DpnII GATC 2 cut(s) 422, 514
DraI TTTAAA 2 cut(s) 180, 1081
DraIII CACNNNGTG 1 cut(s) 763
EaeI YGGCCR 1 cut(s) 312
EagI CGGCCG 1 cut(s) 312
Eam1104I CTCTTC 1 cut(s) 654
EarI CTCTTC 1 cut(s) 654
EclXI CGGCCG 1 cut(s) 312
Eco130I CCWWGG 1 cut(s) 1130
Eco24I GRGCYC 1 cut(s) 36
Eco52I CGGCCG 1 cut(s) 312
Eco57I CTGAAG 1 cut(s) 597
Eco88I CYCGRG 1 cut(s) 111
EcoO109I RGGNCCY 1 cut(s) 32
EcoT14I CCWWGG 1 cut(s) 1130
EcoT38I GRGCYC 1 cut(s) 36
EgeI GGCGCC 1 cut(s) 354
EheI GGCGCC 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 1130
FaeI CATG 5 cut(s) 764, 775, 836, 1165, 1191
FatI CATG 5 cut(s) 760, 771, 832, 1161, 1187
FblI GTMKAC 1 cut(s) 485
FokI GGATG 1 cut(s) 186
FriOI GRGCYC 1 cut(s) 36
GlaI GCGC 3 cut(s) 354, 506, 780
GsaI CCCAGC 2 cut(s) 39, 911
HaeII RGCGCY 2 cut(s) 356, 508
HaeIII GGCC 4 cut(s) 34, 314, 323, 1157
HapII CCGG 3 cut(s) 18, 203, 311
HhaI GCGC 3 cut(s) 355, 507, 781
Hin1I GRCGYC 1 cut(s) 353
Hin1II CATG 5 cut(s) 764, 775, 836, 1165, 1191
Hin6I GCGC 3 cut(s) 353, 505, 779
HinP1I GCGC 3 cut(s) 353, 505, 779
HincII GTYRAC 1 cut(s) 486
HindII GTYRAC 1 cut(s) 486
HinfI GANTC 5 cut(s) 4, 86, 487, 562, 1066
HpaII CCGG 3 cut(s) 18, 203, 311
HphI GGTGA 3 cut(s) 63, 749, 886
Hpy166II GTNNAC 3 cut(s) 486, 956, 1012
Hpy188III TCNNGA 1 cut(s) 530
Hpy8I GTNNAC 3 cut(s) 486, 956, 1012
HpyAV CCTTC 1 cut(s) 78
HpyCH4III ACNGT 4 cut(s) 58, 295, 379, 867
HpyCH4IV ACGT 2 cut(s) 175, 726
HpyCH4V TGCA 7 cut(s) 688, 730, 737, 901, 1165, 1181, 1187
HpyF10VI GCNNNNNNNGC 6 cut(s) 73, 320, 335, 458, 740, 1154
HpyF3I CTNAG 5 cut(s) 330, 500, 714, 797, 1152
HpySE526I ACGT 2 cut(s) 175, 726
Hsp92I GRCGYC 1 cut(s) 353
Hsp92II CATG 5 cut(s) 764, 775, 836, 1165, 1191
HspAI GCGC 3 cut(s) 353, 505, 779
KasI GGCGCC 1 cut(s) 352
Kzo9I GATC 2 cut(s) 422, 514
LmnI GCTCC 1 cut(s) 1120
LweI GCATC 4 cut(s) 23, 439, 629, 1180
MaeII ACGT 2 cut(s) 175, 726
MaeIII GTNAC 4 cut(s) 158, 289, 672, 785
MalI GATC 2 cut(s) 424, 516
MboI GATC 2 cut(s) 422, 514
MboII GAAGA 5 cut(s) 432, 505, 671, 718, 848
MfeI CAATTG 1 cut(s) 154
MhlI GDGCHC 4 cut(s) 36, 221, 552, 768
Mly113I GGCGCC 1 cut(s) 353
MlyI GAGTC 2 cut(s) 80, 481
MmeI TCCRAC 1 cut(s) 116
MslI CAYNNNNRTG 1 cut(s) 1176
MspA1I CMGCKG 3 cut(s) 350, 740, 1184
MspCI CTTAAG 1 cut(s) 592
MspI CCGG 3 cut(s) 18, 203, 311
MunI CAATTG 1 cut(s) 154
MvnI CGCG 1 cut(s) 781
MwoI GCNNNNNNNGC 6 cut(s) 73, 320, 335, 458, 740, 1154
NarI GGCGCC 1 cut(s) 353
NdeII GATC 2 cut(s) 422, 514
NlaIII CATG 5 cut(s) 764, 775, 836, 1165, 1191
NlaIV GGNNCC 2 cut(s) 34, 354
NmeAIII GCCGAG 1 cut(s) 305
NmuCI GTSAC 2 cut(s) 158, 785
NspI RCATGY 1 cut(s) 1165
PaeR7I CTCGAG 1 cut(s) 111
PcsI WCGNNNNNNNCGW 1 cut(s) 383
PfeI GAWTC 3 cut(s) 4, 562, 1066
Pfl23II CGTACG 1 cut(s) 48
PleI GAGTC 2 cut(s) 80, 481
PluTI GGCGCC 1 cut(s) 356
PpsI GAGTC 2 cut(s) 80, 481
Ppu21I YACGTR 1 cut(s) 727
PsiI TTATAA 1 cut(s) 131
PspFI CCCAGC 2 cut(s) 35, 907
PspLI CGTACG 1 cut(s) 48
PspN4I GGNNCC 2 cut(s) 34, 354
PspOMI GGGCCC 1 cut(s) 32
PspPI GGNCC 3 cut(s) 32, 33, 1156
PspXI VCTCGAGB 1 cut(s) 111
PsrI GAACNNNNNNTAC 2 cut(s) 572, 604
PstI CTGCAG 1 cut(s) 739
PstNI CAGNNNCTG 1 cut(s) 299
PvuII CAGCTG 2 cut(s) 740, 1184
RsaI GTAC 3 cut(s) 50, 63, 629
RsaNI GTAC 3 cut(s) 49, 62, 628
RseI CAYNNNNRTG 1 cut(s) 1176
SalI GTCGAC 1 cut(s) 484
Sau3AI GATC 2 cut(s) 422, 514
Sau96I GGNCC 3 cut(s) 32, 33, 1156
ScaI AGTACT 2 cut(s) 63, 629
SchI GAGTC 2 cut(s) 80, 481
SduI GDGCHC 4 cut(s) 36, 221, 552, 768
SfaNI GCATC 4 cut(s) 23, 439, 629, 1180
SfcI CTRYAG 1 cut(s) 735
SfiI GGCCNNNNNGGCC 1 cut(s) 320
SfoI GGCGCC 1 cut(s) 354
Sfr274I CTCGAG 1 cut(s) 111
SlaI CTCGAG 1 cut(s) 111
SmiMI CAYNNNNRTG 1 cut(s) 1176
SmlI CTYRAG 3 cut(s) 111, 530, 592
SmoI CTYRAG 3 cut(s) 111, 530, 592
SsiI CCGC 5 cut(s) 12, 40, 74, 350, 356
SspDI GGCGCC 1 cut(s) 352
StyI CCWWGG 1 cut(s) 1130
TaaI ACNGT 4 cut(s) 58, 295, 379, 867
TaiI ACGT 2 cut(s) 178, 729
TaqI TCGA 3 cut(s) 112, 425, 485
TatI WGTACW 2 cut(s) 61, 627
TauI GCSGC 4 cut(s) 14, 42, 353, 358
TfiI GAWTC 3 cut(s) 4, 562, 1066
TscAI CASTG 2 cut(s) 300, 657
TseFI GTSAC 2 cut(s) 158, 785
Tsp45I GTSAC 2 cut(s) 158, 785
TspDTI ATGAA 5 cut(s) 176, 188, 372, 849, 922
TspRI CASTG 2 cut(s) 300, 657
Vha464I CTTAAG 1 cut(s) 592
XapI RAATTY 2 cut(s) 181, 228
XceI RCATGY 1 cut(s) 1165
XhoI CTCGAG 1 cut(s) 111
XmiI GTMKAC 1 cut(s) 485
ZrmI AGTACT 2 cut(s) 63, 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.