pycom14g04680

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0014496
Physical Location & Seq
Reverse (-)
4397550 .. 4398737
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
ATGGAAGCTATCACTGCTCCAACTCCTACTTGTAATACTACTACTGCTGCTGCATCTGCAAGTGTTCAACATGTGACCAAGAAGTCTTCCGACGAGCTGCTGAGGAAATTTGCGGATTCCGGGGATGAGGCTGAGGTTCCTGCGAGGAAACAGCTGGCGGTGAGGGTGTCGAAGCGTCGGAAAAGAAGCCGGGTGAACGGGGATGGGGATCAGCGGTGTGAGAGCCCATTGAGTGGGAAGAGTGGATTGGTGGAGAGGAGGTCTCTGCTTCCTGCTGGGACTAAAAGCAAGGCGTTGTTTAGGAAGATTGGGATTCATGGCGGCAGGGCTTCACACCTCAGGGCTAGGGATATCAGGAACAAGTCCATCTTTGGCACAATTCACAAGACATGGAGAATGGCCATTGAAGGAGCTTCAAAGGTTTTCATGGAAAAGCATTACAACAGGCACAAGCGTTTGATCGATCACATTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.54

Weight (kDa)

10.82

Isoelectric Point (pI)

56.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42110
fragaria_vesca FvH4_6g22320
malus_domestica MD12G1057100.v1.1 MD14G1057700.v1.1
prunus_persica Prupe.7G077000_v2.0.a1
pyrus_communis pycom12g05100 pycom14g04680
rosa_chinensis RchiOBHm_Chr3g0479681
rosa_laevigata RLG00000023548
rosa_multiflora Rmu_sc0003755.1_g000005
rosa_roxburghii Rroxscaffold_6G00402070
rosa_rugosa Rorug03G0177400
rosa_samantha Rh3AG228100 Rh3BG261300 Rh3CG258400 Rh3DG254600
rosa_wichuraiana Rw3G020410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 82
AccB7I CCANNNNNTGG 1 cut(s) 233
AciI CCGC 4 cut(s) 113, 158, 214, 321
AclWI GGATC 1 cut(s) 216
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 1 cut(s) 107
AfiI CCNNNNNNNGG 1 cut(s) 233
AflIII ACRYGT 1 cut(s) 70
AgsI TTSAA 3 cut(s) 68, 407, 417
AjuI GAANNNNNNNTTGG 2 cut(s) 230, 262
AluBI AGCT 4 cut(s) 8, 97, 154, 413
AluI AGCT 4 cut(s) 8, 97, 154, 413
Alw26I GTCTC 1 cut(s) 267
AlwI GGATC 1 cut(s) 216
AoxI GGCC 1 cut(s) 399
ApeKI GCWGC 3 cut(s) 47, 50, 97
ApoI RAATTY 1 cut(s) 107
AsuC2I CCSGG 2 cut(s) 121, 191
AsuHPI GGTGA 2 cut(s) 172, 205
AxyI CCTNAGG 1 cut(s) 338
BalI TGGCCA 1 cut(s) 401
BanII GRGCYC 1 cut(s) 227
BbsI GAAGAC 1 cut(s) 78
BbvCI CCTCAGC 2 cut(s) 101, 132
BbvI GCAGC 3 cut(s) 34, 37, 84
BccI CCATC 2 cut(s) 197, 374
BcnI CCSGG 2 cut(s) 121, 191
BcoDI GTCTC 1 cut(s) 267
BfaI CTAG 2 cut(s) 345, 475
BisI GCNGC 4 cut(s) 48, 51, 98, 322
BlsI GCNGC 4 cut(s) 49, 52, 99, 323
Bme1390I CCNGG 2 cut(s) 121, 191
BmiI GGNNCC 1 cut(s) 138
BmrFI CCNGG 2 cut(s) 121, 191
BmsI GCATC 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 78
BplI GAGNNNNNCTC 2 cut(s) 247, 279
Bpu10I CCTNAGC 2 cut(s) 101, 132
BpuMI CCSGG 2 cut(s) 121, 191
Bsa29I ATCGAT 1 cut(s) 462
BsaBI GATNNNNATC 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 120
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse21I CCTNAGG 1 cut(s) 338
Bse8I GATNNNNATC 1 cut(s) 207
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 2 cut(s) 130, 208
BseJI GATNNNNATC 1 cut(s) 207
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMII CTCAG 3 cut(s) 92, 123, 352
BseRI GAGGAG 1 cut(s) 271
BseXI GCAGC 3 cut(s) 34, 37, 84
BseYI CCCAGC 1 cut(s) 275
BshFI GGCC 1 cut(s) 401
BshVI ATCGAT 1 cut(s) 462
BsiSI CCGG 2 cut(s) 120, 190
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 1 cut(s) 267
BsmFI GGGAC 1 cut(s) 292
BsnI GGCC 1 cut(s) 401
Bso31I GGTCTC 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 227
Bsp143I GATC 3 cut(s) 208, 459, 463
BspACI CCGC 4 cut(s) 113, 158, 214, 321
BspANI GGCC 1 cut(s) 401
BspCNI CTCAG 3 cut(s) 93, 124, 351
BspDI ATCGAT 1 cut(s) 462
BspLI GGNNCC 1 cut(s) 138
BspPI GGATC 1 cut(s) 216
BspTNI GGTCTC 1 cut(s) 267
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 3 cut(s) 208, 459, 463
Bst6I CTCTTC 1 cut(s) 233
BstC8I GCNNGC 1 cut(s) 156
BstDEI CTNAG 3 cut(s) 101, 132, 338
BstF5I GGATG 2 cut(s) 130, 208
BstKTI GATC 3 cut(s) 211, 462, 466
BstMAI GTCTC 1 cut(s) 267
BstMBI GATC 3 cut(s) 208, 459, 463
BstMWI GCNNNNNNNGC 2 cut(s) 14, 56
BstNSI RCATGY 1 cut(s) 74
BstSCI CCNGG 2 cut(s) 119, 189
BstV1I GCAGC 3 cut(s) 34, 37, 84
BstV2I GAAGAC 1 cut(s) 78
Bsu15I ATCGAT 1 cut(s) 462
Bsu36I CCTNAGG 1 cut(s) 338
BsuRI GGCC 1 cut(s) 401
BsuTUI ATCGAT 1 cut(s) 462
BtsCI GGATG 2 cut(s) 130, 208
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 1 cut(s) 12
Cac8I GCNNGC 1 cut(s) 156
ClaI ATCGAT 1 cut(s) 462
CseI GACGC 1 cut(s) 164
CviAII CATG 4 cut(s) 71, 317, 390, 427
DdeI CTNAG 3 cut(s) 101, 132, 338
DpnI GATC 3 cut(s) 210, 461, 465
DpnII GATC 3 cut(s) 208, 459, 463
DrdI GACNNNNNNGTC 1 cut(s) 82
DseDI GACNNNNNNGTC 1 cut(s) 82
EaeI YGGCCR 1 cut(s) 399
Eam1104I CTCTTC 1 cut(s) 233
EarI CTCTTC 1 cut(s) 233
Eco24I GRGCYC 1 cut(s) 227
Eco31I GGTCTC 1 cut(s) 267
Eco32I GATATC 1 cut(s) 352
Eco81I CCTNAGG 1 cut(s) 338
EcoRV GATATC 1 cut(s) 352
EcoT38I GRGCYC 1 cut(s) 227
FaeI CATG 4 cut(s) 74, 320, 393, 430
FaiI YATR 4 cut(s) 72, 318, 391, 428
FalI AAGNNNNNCTT 2 cut(s) 353, 385
FaqI GGGAC 1 cut(s) 292
FatI CATG 4 cut(s) 70, 316, 389, 426
Fnu4HI GCNGC 4 cut(s) 48, 51, 98, 322
FokI GGATG 2 cut(s) 137, 215
FriOI GRGCYC 1 cut(s) 227
Fsp4HI GCNGC 4 cut(s) 48, 51, 98, 322
FspBI CTAG 2 cut(s) 345, 475
GluI GCNGC 4 cut(s) 48, 51, 98, 322
GsaI CCCAGC 1 cut(s) 279
HaeIII GGCC 1 cut(s) 401
HapII CCGG 2 cut(s) 120, 190
HgaI GACGC 1 cut(s) 164
Hin1II CATG 4 cut(s) 74, 320, 393, 430
HinfI GANTC 2 cut(s) 116, 313
HpaII CCGG 2 cut(s) 120, 190
HphI GGTGA 2 cut(s) 172, 205
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 2 cut(s) 91, 180
Hpy188III TCNNGA 1 cut(s) 355
Hpy8I GTNNAC 1 cut(s) 196
Hpy99I CGWCG 2 cut(s) 95, 180
HpyAV CCTTC 1 cut(s) 401
HpyCH4V TGCA 2 cut(s) 53, 59
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 56
HpyF3I CTNAG 3 cut(s) 101, 132, 338
Hsp92II CATG 4 cut(s) 74, 320, 393, 430
Kzo9I GATC 3 cut(s) 208, 459, 463
LmnI GCTCC 2 cut(s) 22, 410
Lsp1109I GCAGC 3 cut(s) 34, 37, 84
LweI GCATC 1 cut(s) 62
MaeI CTAG 2 cut(s) 345, 475
MaeIII GTNAC 1 cut(s) 73
MalI GATC 3 cut(s) 210, 461, 465
MboI GATC 3 cut(s) 208, 459, 463
MboII GAAGA 3 cut(s) 78, 250, 316
MhlI GDGCHC 1 cut(s) 227
MlsI TGGCCA 1 cut(s) 401
MluCI AATT 2 cut(s) 107, 378
MluNI TGGCCA 1 cut(s) 401
MmeI TCCRAC 3 cut(s) 44, 114, 158
MnlI CCTC 8 cut(s) 96, 121, 127, 138, 156, 249, 252, 347
Mox20I TGGCCA 1 cut(s) 401
MscI TGGCCA 1 cut(s) 401
Msp20I TGGCCA 1 cut(s) 401
MspA1I CMGCKG 2 cut(s) 154, 214
MspI CCGG 2 cut(s) 120, 190
MspR9I CCNGG 2 cut(s) 121, 191
MwoI GCNNNNNNNGC 2 cut(s) 14, 56
NciI CCSGG 2 cut(s) 121, 191
NdeII GATC 3 cut(s) 208, 459, 463
NlaIII CATG 4 cut(s) 74, 320, 393, 430
NlaIV GGNNCC 1 cut(s) 138
NmuCI GTSAC 1 cut(s) 73
NspI RCATGY 1 cut(s) 74
PciI ACATGT 1 cut(s) 70
PfeI GAWTC 2 cut(s) 116, 313
PflMI CCANNNNNTGG 1 cut(s) 233
PkrI GCNGC 4 cut(s) 49, 52, 99, 323
PscI ACATGT 1 cut(s) 70
PspFI CCCAGC 1 cut(s) 275
PspN4I GGNNCC 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 154
SatI GCNGC 4 cut(s) 48, 51, 98, 322
Sau3AI GATC 3 cut(s) 208, 459, 463
ScrFI CCNGG 2 cut(s) 121, 191
SduI GDGCHC 1 cut(s) 227
SetI ASST 8 cut(s) 10, 99, 138, 156, 263, 339, 415, 423
SfaNI GCATC 1 cut(s) 62
Sse9I AATT 2 cut(s) 107, 378
SsiI CCGC 4 cut(s) 113, 158, 214, 321
SspMI CTAG 2 cut(s) 345, 475
StyD4I CCNGG 2 cut(s) 119, 189
TaqI TCGA 2 cut(s) 170, 462
TasI AATT 2 cut(s) 107, 378
TauI GCSGC 1 cut(s) 324
TfiI GAWTC 2 cut(s) 116, 313
TscAI CASTG 1 cut(s) 19
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 3 cut(s) 47, 50, 97
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 2 cut(s) 305, 415
TspRI CASTG 1 cut(s) 19
Van91I CCANNNNNTGG 1 cut(s) 233
XapI RAATTY 1 cut(s) 107
XceI RCATGY 1 cut(s) 74
XspI CTAG 2 cut(s) 345, 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.