pycom14g04800

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001598
Physical Location & Seq
Reverse (-)
4493146 .. 4497540
4395 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1122 bp
ATGGACTATGATGTCTCCGAAATCAGTGGCACTGATGATGATCTTCCTCCATCTCATCAAAACAGAATTCCTCGAGGGGGTAAAGTCTCGGGAAACGAAAGATCTGCTGTAGGTTCTATCTCATTTTCTAGGATGTATACAGACATGGAGGCTGAAATTCATCACCTTGAGCAAGAAGCATACTGTGCAGTCCTAAGGGCGTTTAAAGCGCAGTCTAATGCGATTACTTGGGAGAAGGAAGGTTTGATTACTGAACTTAGGAAAGAACTGAGAGTATCGGATGATGAACACAGAGAGCTTCTGACCATGGTTAATTCTGAGGACATCATCGATAAGATAAGAGTGTGGAGAGACAAAGGTGGTCATCAAGCTGCTAGGCTTACTTCATCTCAGAATGTATATGAACTTTTACCTAGTCCTAGTATTTCAGCGTCCCGAAAGAAACAGAAAACATCACAATCGCATGGTCAGCCATTCCCTGGTTTATCCTCAATGAAGTCCATGAAATACCCTTCAACAGGACCTGTCGGAAGTAGACAATTCACCCGCAGTTCTTCTGGTCCCTTTGTTGAAGCGGCTGAAGCAGAAACGTCTGATTCATTAGTTGGAAGGAAAGTGTGGACAAGGTGGCCTGAAGACAACAGCTTCTATGAAGCCGTTATCACTTACTACAACCCGGCAGAGGGTCGACACGCTTTGGTCTACGACATACATACACCAAATGAGACATGGGAATGGGTTGATCTTAAAGAGATCTCTCCTGAGGATATCTGTTGGGAAGATCCGGGCCCATCAGGACGCAGGGCTAAGAAATCTACGAGCCATACCACAAGAAAGCGAGGACCCACAAAGTCTCAATCAAAGAAAGAACTTCCGTCGCAAAATGGTATAGGGAAGGCGCCAGATGATTTAGAATTATTAAATACCGATACCCTGGTGAAGGAGGTGGAGAGAGTTTTCAATGCAAGTGAGCCAGATCCTTTTGAGCTCGAAAAAGCAAGGAAAATTCTGAAAGATCACGAACAGGCCCTTGTTGATGCCATTCAAAGGCTGGCATATGCATCGGATGGTGAAAGTGATAGAGAGCACACATTTCTGCACAGTCGATCAATGGATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

374

Amino Acids

42.03

Weight (kDa)

5.64

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 51 - 119 9.6e-27 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 11
AbsI CCTCGAGG 1 cut(s) 72
AccB1I GGYRCC 1 cut(s) 898
AccB7I CCANNNNNTGG 1 cut(s) 479
AccI GTMKAC 4 cut(s) 137, 535, 688, 702
AciI CCGC 2 cut(s) 547, 575
AclWI GGATC 2 cut(s) 776, 971
AcsI RAATTY 3 cut(s) 66, 156, 1005
AcuI CTGAAG 2 cut(s) 600, 654
AcyI GRCGYC 1 cut(s) 899
AfiI CCNNNNNNNGG 7 cut(s) 77, 479, 518, 682, 683, 940, 1047
AgsI TTSAA 4 cut(s) 516, 572, 961, 1046
AjnI CCWGG 2 cut(s) 478, 933
AluBI AGCT 4 cut(s) 298, 371, 645, 988
AluI AGCT 4 cut(s) 298, 371, 645, 988
Alw21I GWGCWC 2 cut(s) 990, 1089
Alw26I GTCTC 5 cut(s) 19, 91, 345, 719, 858
AlwI GGATC 2 cut(s) 776, 971
AlwNI CAGNNNCTG 1 cut(s) 524
Ama87I CYCGRG 2 cut(s) 72, 88
AoxI GGCC 3 cut(s) 629, 787, 1026
ApaI GGGCCC 1 cut(s) 791
ApeKI GCWGC 1 cut(s) 371
ApoI RAATTY 3 cut(s) 66, 156, 1005
AspLEI GCGC 2 cut(s) 211, 901
AspS9I GGNCC 6 cut(s) 521, 560, 787, 788, 842, 1027
AsuC2I CCSGG 2 cut(s) 677, 786
AsuHPI GGTGA 4 cut(s) 155, 535, 949, 1082
AvaI CYCGRG 2 cut(s) 72, 88
AvaII GGWCC 3 cut(s) 521, 560, 842
AxyI CCTNAGG 2 cut(s) 194, 762
BaeGI GKGCMC 1 cut(s) 791
BaeI ACNNNNGTAYC 2 cut(s) 921, 954
BanI GGYRCC 1 cut(s) 898
BanII GRGCYC 2 cut(s) 791, 990
BbsI GAAGAC 1 cut(s) 642
Bbv12I GWGCWC 2 cut(s) 990, 1089
BbvI GCAGC 1 cut(s) 358
BccI CCATC 4 cut(s) 58, 799, 1061, 1109
BceAI ACGGC 1 cut(s) 641
BcgI CGANNNNNNTGC 4 cut(s) 86, 120, 1044, 1078
BciT130I CCWGG 2 cut(s) 480, 935
BcnI CCSGG 2 cut(s) 677, 786
BcoDI GTCTC 5 cut(s) 19, 91, 345, 719, 858
BfaI CTAG 4 cut(s) 129, 375, 414, 420
BfmI CTRYAG 1 cut(s) 108
BfoI RGCGCY 1 cut(s) 902
BglII AGATCT 2 cut(s) 101, 753
BisI GCNGC 2 cut(s) 372, 576
BlsI GCNGC 2 cut(s) 373, 577
Bme1390I CCNGG 4 cut(s) 480, 677, 786, 935
Bme18I GGWCC 3 cut(s) 521, 560, 842
BmeT110I CYCGRG 2 cut(s) 72, 88
BmgT120I GGNCC 6 cut(s) 521, 560, 787, 788, 842, 1027
BmiI GGNNCC 4 cut(s) 562, 789, 844, 900
BmrFI CCNGG 4 cut(s) 480, 677, 786, 935
BmsI GCATC 2 cut(s) 1027, 1070
BpiI GAAGAC 1 cut(s) 642
BpuEI CTTGAG 1 cut(s) 188
BpuMI CCSGG 2 cut(s) 677, 786
Bsa29I ATCGAT 1 cut(s) 330
BsaBI GATNNNNATC 1 cut(s) 39
BsaHI GRCGYC 1 cut(s) 899
BsaJI CCNNGG 3 cut(s) 306, 478, 933
Bsc4I CCNNNNNNNGG 7 cut(s) 77, 479, 518, 682, 683, 940, 1047
Bse21I CCTNAGG 2 cut(s) 194, 762
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 2 cut(s) 480, 935
BseCI ATCGAT 1 cut(s) 330
BseDI CCNNGG 3 cut(s) 306, 478, 933
BseGI GGATG 4 cut(s) 138, 286, 1072, 1120
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 7 cut(s) 77, 479, 518, 682, 683, 940, 1047
BseMII CTCAG 4 cut(s) 260, 309, 404, 753
BseSI GKGCMC 1 cut(s) 791
BseXI GCAGC 1 cut(s) 358
BsgI GTGCAG 2 cut(s) 207, 1082
BshFI GGCC 3 cut(s) 631, 789, 1028
BshNI GGYRCC 1 cut(s) 898
BshVI ATCGAT 1 cut(s) 330
BsiHKAI GWGCWC 2 cut(s) 990, 1089
BsiHKCI CYCGRG 2 cut(s) 72, 88
BsiSI CCGG 2 cut(s) 677, 785
BslFI GGGAC 2 cut(s) 418, 546
BslI CCNNNNNNNGG 7 cut(s) 77, 479, 518, 682, 683, 940, 1047
BsmAI GTCTC 5 cut(s) 19, 91, 345, 719, 858
BsmFI GGGAC 2 cut(s) 418, 546
BsnI GGCC 3 cut(s) 631, 789, 1028
BsoBI CYCGRG 2 cut(s) 72, 88
Bsp120I GGGCCC 1 cut(s) 787
Bsp1286I GDGCHC 3 cut(s) 791, 990, 1089
Bsp143I GATC 8 cut(s) 40, 101, 742, 753, 781, 976, 1015, 1106
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 2 cut(s) 547, 575
BspANI GGCC 3 cut(s) 631, 789, 1028
BspCNI CTCAG 4 cut(s) 261, 310, 403, 754
BspDI ATCGAT 1 cut(s) 330
BspLI GGNNCC 4 cut(s) 562, 789, 844, 900
BspPI GGATC 2 cut(s) 776, 971
BspT107I GGYRCC 1 cut(s) 898
BssECI CCNNGG 3 cut(s) 306, 478, 933
BssMI GATC 8 cut(s) 40, 101, 742, 753, 781, 976, 1015, 1106
BssNAI GTATAC 1 cut(s) 138
BssNI GRCGYC 1 cut(s) 899
BssT1I CCWWGG 1 cut(s) 306
Bst1107I GTATAC 1 cut(s) 138
Bst2UI CCWGG 2 cut(s) 480, 935
Bst4CI ACNGT 2 cut(s) 185, 1103
BstACI GRCGYC 1 cut(s) 899
BstAPI GCANNNNNTGC 1 cut(s) 185
BstC8I GCNNGC 1 cut(s) 1053
BstDEI CTNAG 7 cut(s) 194, 257, 269, 318, 390, 762, 807
BstDSI CCRYGG 1 cut(s) 306
BstENI CCTNNNNNAGG 2 cut(s) 516, 938
BstF5I GGATG 4 cut(s) 138, 286, 1072, 1120
BstH2I RGCGCY 1 cut(s) 902
BstHHI GCGC 2 cut(s) 211, 901
BstKTI GATC 8 cut(s) 43, 104, 745, 756, 784, 979, 1018, 1109
BstMAI GTCTC 5 cut(s) 19, 91, 345, 719, 858
BstMBI GATC 8 cut(s) 40, 101, 742, 753, 781, 976, 1015, 1106
BstMWI GCNNNNNNNGC 4 cut(s) 185, 206, 469, 581
BstNI CCWGG 2 cut(s) 480, 935
BstSCI CCNGG 4 cut(s) 478, 675, 784, 933
BstSFI CTRYAG 1 cut(s) 108
BstSLI GKGCMC 1 cut(s) 791
BstV1I GCAGC 1 cut(s) 358
BstV2I GAAGAC 1 cut(s) 642
BstX2I RGATCY 4 cut(s) 101, 753, 781, 976
BstYI RGATCY 4 cut(s) 101, 753, 781, 976
BstZ17I GTATAC 1 cut(s) 138
Bsu15I ATCGAT 1 cut(s) 330
Bsu36I CCTNAGG 2 cut(s) 194, 762
BsuRI GGCC 3 cut(s) 631, 789, 1028
BsuTUI ATCGAT 1 cut(s) 330
BtgI CCRYGG 1 cut(s) 306
BtsCI GGATG 4 cut(s) 138, 286, 1072, 1120
BtsIMutI CAGTG 2 cut(s) 30, 31
Cac8I GCNNGC 1 cut(s) 1053
CaiI CAGNNNCTG 1 cut(s) 524
CfoI GCGC 2 cut(s) 211, 901
Cfr13I GGNCC 6 cut(s) 521, 560, 787, 788, 842, 1027
ClaI ATCGAT 1 cut(s) 330
CseI GACGC 2 cut(s) 420, 807
CviAII CATG 5 cut(s) 145, 307, 464, 502, 729
DdeI CTNAG 7 cut(s) 194, 257, 269, 318, 390, 762, 807
DinI GGCGCC 1 cut(s) 900
DpnI GATC 8 cut(s) 42, 103, 744, 755, 783, 978, 1017, 1108
DpnII GATC 8 cut(s) 40, 101, 742, 753, 781, 976, 1015, 1106
DraI TTTAAA 1 cut(s) 205
DrdI GACNNNNNNGTC 1 cut(s) 11
DseDI GACNNNNNNGTC 1 cut(s) 11
Ecl136II GAGCTC 1 cut(s) 988
Eco130I CCWWGG 1 cut(s) 306
Eco24I GRGCYC 2 cut(s) 791, 990
Eco32I GATATC 1 cut(s) 769
Eco47I GGWCC 3 cut(s) 521, 560, 842
Eco53kI GAGCTC 1 cut(s) 988
Eco57I CTGAAG 2 cut(s) 600, 654
Eco81I CCTNAGG 2 cut(s) 194, 762
Eco88I CYCGRG 2 cut(s) 72, 88
EcoICRI GAGCTC 1 cut(s) 988
EcoNI CCTNNNNNAGG 2 cut(s) 516, 938
EcoO109I RGGNCCY 3 cut(s) 521, 842, 1027
EcoRI GAATTC 1 cut(s) 66
EcoRII CCWGG 2 cut(s) 478, 933
EcoRV GATATC 1 cut(s) 769
EcoT14I CCWWGG 1 cut(s) 306
EcoT22I ATGCAT 1 cut(s) 1063
EcoT38I GRGCYC 2 cut(s) 791, 990
EgeI GGCGCC 1 cut(s) 900
EheI GGCGCC 1 cut(s) 900
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 5 cut(s) 148, 310, 467, 505, 732
FaqI GGGAC 2 cut(s) 418, 546
FatI CATG 5 cut(s) 144, 306, 463, 501, 728
FauI CCCGC 1 cut(s) 554
FauNDI CATATG 1 cut(s) 1057
FblI GTMKAC 4 cut(s) 137, 535, 688, 702
Fnu4HI GCNGC 2 cut(s) 372, 576
FokI GGATG 3 cut(s) 145, 293, 1079
FriOI GRGCYC 2 cut(s) 791, 990
Fsp4HI GCNGC 2 cut(s) 372, 576
FspBI CTAG 4 cut(s) 129, 375, 414, 420
GlaI GCGC 2 cut(s) 210, 900
GluI GCNGC 2 cut(s) 372, 576
HaeII RGCGCY 1 cut(s) 902
HaeIII GGCC 3 cut(s) 631, 789, 1028
HapII CCGG 2 cut(s) 677, 785
HgaI GACGC 2 cut(s) 420, 807
HhaI GCGC 2 cut(s) 211, 901
Hin1I GRCGYC 1 cut(s) 899
Hin1II CATG 5 cut(s) 148, 310, 467, 505, 732
Hin6I GCGC 2 cut(s) 209, 899
HinP1I GCGC 2 cut(s) 209, 899
HincII GTYRAC 1 cut(s) 689
HindII GTYRAC 1 cut(s) 689
HinfI GANTC 1 cut(s) 596
HpaII CCGG 2 cut(s) 677, 785
HphI GGTGA 4 cut(s) 155, 535, 949, 1082
Hpy166II GTNNAC 5 cut(s) 138, 536, 621, 689, 703
Hpy188I TCNGA 9 cut(s) 19, 280, 303, 319, 393, 530, 595, 1011, 1066
Hpy188III TCNNGA 5 cut(s) 90, 435, 761, 795, 1019
Hpy8I GTNNAC 5 cut(s) 138, 536, 621, 689, 703
Hpy99I CGWCG 1 cut(s) 880
HpyAV CCTTC 6 cut(s) 229, 233, 522, 603, 889, 934
HpyCH4III ACNGT 2 cut(s) 185, 1103
HpyCH4IV ACGT 1 cut(s) 590
HpyCH4V TGCA 4 cut(s) 188, 965, 1061, 1099
HpyF10VI GCNNNNNNNGC 4 cut(s) 185, 206, 469, 581
HpyF3I CTNAG 7 cut(s) 194, 257, 269, 318, 390, 762, 807
HpySE526I ACGT 1 cut(s) 590
Hsp92I GRCGYC 1 cut(s) 899
Hsp92II CATG 5 cut(s) 148, 310, 467, 505, 732
HspAI GCGC 2 cut(s) 209, 899
KasI GGCGCC 1 cut(s) 898
Kzo9I GATC 8 cut(s) 40, 101, 742, 753, 781, 976, 1015, 1106
Lsp1109I GCAGC 1 cut(s) 358
LweI GCATC 2 cut(s) 1027, 1070
MaeI CTAG 4 cut(s) 129, 375, 414, 420
MaeII ACGT 1 cut(s) 590
MalI GATC 8 cut(s) 42, 103, 744, 755, 783, 978, 1017, 1108
MboI GATC 8 cut(s) 40, 101, 742, 753, 781, 976, 1015, 1106
MboII GAAGA 4 cut(s) 35, 546, 647, 791
MflI RGATCY 4 cut(s) 101, 753, 781, 976
MhlI GDGCHC 3 cut(s) 791, 990, 1089
MluCI AATT 6 cut(s) 66, 156, 313, 539, 914, 1005
Mly113I GGCGCC 1 cut(s) 899
MmeI TCCRAC 2 cut(s) 508, 586
Mph1103I ATGCAT 1 cut(s) 1063
MseI TTAA 4 cut(s) 204, 312, 747, 920
MslI CAYNNNNRTG 1 cut(s) 733
MspI CCGG 2 cut(s) 677, 785
MspR9I CCNGG 4 cut(s) 480, 677, 786, 935
MvaI CCWGG 2 cut(s) 480, 935
MwoI GCNNNNNNNGC 4 cut(s) 185, 206, 469, 581
NarI GGCGCC 1 cut(s) 899
NciI CCSGG 2 cut(s) 677, 786
NcoI CCATGG 1 cut(s) 306
NdeI CATATG 1 cut(s) 1057
NdeII GATC 8 cut(s) 40, 101, 742, 753, 781, 976, 1015, 1106
NlaIII CATG 5 cut(s) 148, 310, 467, 505, 732
NlaIV GGNNCC 4 cut(s) 562, 789, 844, 900
NsiI ATGCAT 1 cut(s) 1063
PaeR7I CTCGAG 1 cut(s) 72
PfeI GAWTC 1 cut(s) 596
PflMI CCANNNNNTGG 1 cut(s) 479
PkrI GCNGC 2 cut(s) 373, 577
PluTI GGCGCC 1 cut(s) 902
PpuMI RGGWCCY 2 cut(s) 521, 842
Psp124BI GAGCTC 1 cut(s) 990
Psp5II RGGWCCY 2 cut(s) 521, 842
Psp6I CCWGG 2 cut(s) 478, 933
PspGI CCWGG 2 cut(s) 478, 933
PspN4I GGNNCC 4 cut(s) 562, 789, 844, 900
PspOMI GGGCCC 1 cut(s) 787
PspPI GGNCC 6 cut(s) 521, 560, 787, 788, 842, 1027
PspPPI RGGWCCY 2 cut(s) 521, 842
PspXI VCTCGAGB 1 cut(s) 72
PstNI CAGNNNCTG 1 cut(s) 524
PsuI RGATCY 4 cut(s) 101, 753, 781, 976
RseI CAYNNNNRTG 1 cut(s) 733
SacI GAGCTC 1 cut(s) 990
SalI GTCGAC 1 cut(s) 687
SaqAI TTAA 4 cut(s) 204, 312, 747, 920
SatI GCNGC 2 cut(s) 372, 576
Sau3AI GATC 8 cut(s) 40, 101, 742, 753, 781, 976, 1015, 1106
Sau96I GGNCC 6 cut(s) 521, 560, 787, 788, 842, 1027
ScrFI CCNGG 4 cut(s) 480, 677, 786, 935
SduI GDGCHC 3 cut(s) 791, 990, 1089
SfaNI GCATC 2 cut(s) 1027, 1070
SfcI CTRYAG 1 cut(s) 108
SfoI GGCGCC 1 cut(s) 900
Sfr274I CTCGAG 1 cut(s) 72
SinI GGWCC 3 cut(s) 521, 560, 842
SlaI CTCGAG 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 733
SmlI CTYRAG 2 cut(s) 72, 167
SmoI CTYRAG 2 cut(s) 72, 167
Sse9I AATT 6 cut(s) 66, 156, 313, 539, 914, 1005
SsiI CCGC 2 cut(s) 547, 575
SspDI GGCGCC 1 cut(s) 898
SspMI CTAG 4 cut(s) 129, 375, 414, 420
SstI GAGCTC 1 cut(s) 990
StyD4I CCNGG 4 cut(s) 478, 675, 784, 933
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 2 cut(s) 185, 1103
TaiI ACGT 1 cut(s) 593
TaqI TCGA 5 cut(s) 73, 330, 688, 990, 1105
TasI AATT 6 cut(s) 66, 156, 313, 539, 914, 1005
TauI GCSGC 1 cut(s) 578
TfiI GAWTC 1 cut(s) 596
Tru1I TTAA 4 cut(s) 204, 312, 747, 920
Tru9I TTAA 4 cut(s) 204, 312, 747, 920
TscAI CASTG 2 cut(s) 31, 37
TseI GCWGC 1 cut(s) 371
TspDTI ATGAA 8 cut(s) 149, 300, 375, 417, 509, 518, 588, 666
TspGWI ACGGA 1 cut(s) 864
TspRI CASTG 2 cut(s) 31, 37
Van91I CCANNNNNTGG 1 cut(s) 479
VpaK11BI GGWCC 3 cut(s) 521, 560, 842
XagI CCTNNNNNAGG 2 cut(s) 516, 938
XapI RAATTY 3 cut(s) 66, 156, 1005
XcmI CCANNNNNNNNNTGG 2 cut(s) 726, 1048
XhoI CTCGAG 1 cut(s) 72
XmiI GTMKAC 4 cut(s) 137, 535, 688, 702
XspI CTAG 4 cut(s) 129, 375, 414, 420
Zsp2I ATGCAT 1 cut(s) 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.