pycom14g05060

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004503
Physical Location & Seq
Reverse (-)
4874010 .. 4875993
1984 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g05060.1

Sequence Viewer

Length: 1542 bp
ATGGAAGTAGATTTCCAAGGATACATCATACTTTTCCTCGTGTGGCTAGTCTCCACAGTTTTGGTCCGAACCATGCTCACCAAATTTTGGACCAAGCCTCGCCTTCCACCAAGTCCGATGGCCTTGCCAATCCTCGGACACCTCCACCTCCTTGCTCCAATACCTCACCAAGCTCTTCACAAGCTATCCAACCGATATGGGTCTCTGATTCACCTCTTTCTTGGTTCCGTCCCTTGCGTAGTTGCTTCCACGCCAGAGATGGCGAAAGAGTTCCTCAAAACTCATGAGGGTTCTTTCTCCAACAGGCCCTATATTTCTGCTGTTGATTACCTCACGTACGGATCAGCTGATTTTTCATTTGCTCCTTATGGACCTTATTGGCAGTTCATGAAGAAACTATGCATGTCCGAGCTTCTTGGTGGAAGGACACTCGATCAGCTCCTTCCAGTCCGCCGTGAAGAGTTGGCGAGTTTCGTGCAACTGCTTCTGAACAAGGCCAAGGCAGCCGAGCCAGTTGATGTTGGCGCAGAGCTTATGACGGTGACAAATAACGTCATATCGAGAATGATTATGGGGCAGAGTTGCTCTTCGAACGAAAAAGAGGCTGATGAGATCAGGAAGCAGGTGAAAGCTATTGCTGAGTTGACGGGAAAGTTTAATTTGTCAGACTTCATATGGTTTTGTAAGAATTTGGATTTGCAAGGGTTTGGGAAACAGCTTAAGGAAGTGCGTGACAGGTTTGACTTTATGATGGAGAGGATTATCAAGGAGCACCAAGAGGAAAGGAAGAAGAAGGAAGTTGGCGAAGGTGGCGGTGATGCAGTTAAGGATCTACTTGACATTTTACTCGACATAGCTGAAGATGAAACCTCGGAGTTTAGATTGACAAGAGAAAACATAAAGGCATTCATCCTGGATATATTTTCTGCTGGAACCGACACATCTGCAATCACGACAGAATGGGCACTAGCAGAACTAATCAACCATCCAGATGTGATGCAGAAAGCAAGACAAGAAATTGACACCATAATTGGAACAAACAGGCTAGTACAAGAATCAGACATTGCCAACCTTCCCTACCTACAAGCCATAGTGAAAGAAACCCTAAGGCTTCACCCAACCGGTCCATTAATCGTGAGGGAGTCCTCCGAATTTTGCACCATCGGCGGCTATGACATTCCCGCGAAAACAAGATTATTTGTGAATGTGTGGGCTATCAACAGAGACCCCAACCACTGGGTGACGCCATTGGAGTTTGAGCCGGAGAGGTTTGTGACCGAAGAGGGAAGTGGGAAGAGCCAGTTAGATGTGAGGGGGCAGCATTTTCATCTGTTGCCATTTGGGAGTGGACGAAGAGGATGTCCCGGAACCTCGCTTGCAATGCAGGTTGTGCAGACAACACTTGCCGCTATGGTTCAGTGCTTTGAGTGGAAAGTTGAAGGAACTGTTGACATGGAAGAGGCAGCTGGATTAACACTTCCGAGGGCTCATCCTTTGGTTTGTGTTCCAGTGGCTAGGTTCAATCCCGTTCCATCTTCCTGA

Protein Analysis

514

Amino Acids

57.68

Weight (kDa)

5.79

Isoelectric Point (pI)

30.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 36 - 495 8.6e-106 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 613
Acc36I ACCTGC 2 cut(s) 613, 1375
AccB7I CCANNNNNTGG 2 cut(s) 87, 1236
AccII CGCG 1 cut(s) 1184
AciI CCGC 5 cut(s) 451, 813, 1167, 1182, 1407
AclWI GGATC 2 cut(s) 349, 837
AcsI RAATTY 3 cut(s) 83, 688, 1151
AcuI CTGAAG 1 cut(s) 879
AcyI GRCGYC 1 cut(s) 1244
AdeI CACNNNGTG 1 cut(s) 1240
AfaI GTAC 2 cut(s) 338, 1050
AfiI CCNNNNNNNGG 3 cut(s) 87, 134, 1236
AflII CTTAAG 1 cut(s) 719
AgeI ACCGGT 1 cut(s) 1121
AgsI TTSAA 2 cut(s) 1439, 1522
AjnI CCWGG 1 cut(s) 912
Alw21I GWGCWC 1 cut(s) 774
Alw26I GTCTC 3 cut(s) 55, 207, 1218
AlwI GGATC 2 cut(s) 349, 837
AoxI GGCC 3 cut(s) 120, 305, 495
ApeKI GCWGC 3 cut(s) 503, 1318, 1463
ApoI RAATTY 3 cut(s) 83, 688, 1151
ArsI GACNNNNNNTTYG 2 cut(s) 1345, 1377
AseI ATTAAT 1 cut(s) 1130
AsiGI ACCGGT 1 cut(s) 1121
Asp700I GAANNNNTTC 1 cut(s) 269
AspLEI GCGC 1 cut(s) 527
AspS9I GGNCC 5 cut(s) 64, 90, 306, 371, 1124
AsuC2I CCSGG 1 cut(s) 1365
AsuHPI GGTGA 8 cut(s) 70, 158, 203, 553, 637, 827, 1106, 1252
AsuII TTCGAA 1 cut(s) 590
AvaII GGWCC 4 cut(s) 64, 90, 371, 1124
AxyI CCTNAGG 1 cut(s) 1106
BaeGI GKGCMC 1 cut(s) 967
BanII GRGCYC 1 cut(s) 1489
BauI CACGAG 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 774
BbvI GCAGC 3 cut(s) 515, 1330, 1475
BccI CCATC 6 cut(s) 112, 253, 745, 993, 1169, 1540
BceAI ACGGC 1 cut(s) 438
BcgI CGANNNNNNTGC 6 cut(s) 106, 140, 457, 491, 926, 960
BciT130I CCWGG 1 cut(s) 914
BciVI GTATCC 1 cut(s) 14
BcnI CCSGG 1 cut(s) 1365
BcoDI GTCTC 3 cut(s) 55, 207, 1218
BfaI CTAG 4 cut(s) 47, 968, 1046, 1515
BfrI CTTAAG 1 cut(s) 719
BfuAI ACCTGC 2 cut(s) 613, 1375
BfuI GTATCC 1 cut(s) 14
BisI GCNGC 5 cut(s) 504, 1168, 1319, 1407, 1464
BlsI GCNGC 5 cut(s) 505, 1169, 1320, 1408, 1465
Bme1390I CCNGG 2 cut(s) 914, 1365
Bme18I GGWCC 4 cut(s) 64, 90, 371, 1124
BmgT120I GGNCC 5 cut(s) 64, 90, 306, 371, 1124
BmiI GGNNCC 3 cut(s) 226, 934, 1369
BmrFI CCNGG 2 cut(s) 914, 1365
BmrI ACTGGG 1 cut(s) 1246
BmsI GCATC 2 cut(s) 808, 987
BmuI ACTGGG 1 cut(s) 1246
Bpu14I TTCGAA 1 cut(s) 590
BpuMI CCSGG 1 cut(s) 1365
BsaAI YACGTR 1 cut(s) 336
BsaHI GRCGYC 1 cut(s) 1244
BsaI GGTCTC 2 cut(s) 207, 1218
BsaJI CCNNGG 5 cut(s) 16, 133, 498, 870, 1481
BsaWI WCCGGW 1 cut(s) 1121
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 134, 1236
Bse118I RCCGGY 1 cut(s) 1121
Bse1I ACTGG 5 cut(s) 446, 512, 1241, 1300, 1508
Bse21I CCTNAGG 1 cut(s) 1106
Bse3DI GCAATG 2 cut(s) 1062, 1386
BseBI CCWGG 1 cut(s) 914
BseDI CCNNGG 5 cut(s) 16, 133, 498, 870, 1481
BseGI GGATG 4 cut(s) 909, 985, 1364, 1489
BseLI CCNNNNNNNGG 3 cut(s) 87, 134, 1236
BseMI GCAATG 2 cut(s) 1062, 1386
BseMII CTCAG 1 cut(s) 630
BseNI ACTGG 5 cut(s) 446, 512, 1241, 1300, 1508
BseSI GKGCMC 1 cut(s) 967
BseXI GCAGC 3 cut(s) 515, 1330, 1475
BsgI GTGCAG 1 cut(s) 1412
Bsh1236I CGCG 1 cut(s) 1184
BshFI GGCC 3 cut(s) 122, 307, 497
BshTI ACCGGT 1 cut(s) 1121
BsiHKAI GWGCWC 1 cut(s) 774
BsiSI CCGG 3 cut(s) 1122, 1262, 1365
BsiWI CGTACG 1 cut(s) 336
BslFI GGGAC 2 cut(s) 215, 1347
BslI CCNNNNNNNGG 3 cut(s) 87, 134, 1236
BsmAI GTCTC 3 cut(s) 55, 207, 1218
BsmFI GGGAC 2 cut(s) 215, 1347
BsmI GAATGC 1 cut(s) 905
BsnI GGCC 3 cut(s) 122, 307, 497
Bso31I GGTCTC 2 cut(s) 207, 1218
Bsp119I TTCGAA 1 cut(s) 590
Bsp1286I GDGCHC 3 cut(s) 774, 967, 1489
Bsp143I GATC 4 cut(s) 341, 433, 612, 829
BspACI CCGC 5 cut(s) 451, 813, 1167, 1182, 1407
BspANI GGCC 3 cut(s) 122, 307, 497
BspCNI CTCAG 1 cut(s) 631
BspFNI CGCG 1 cut(s) 1184
BspHI TCATGA 2 cut(s) 283, 387
BspLI GGNNCC 3 cut(s) 226, 934, 1369
BspMI ACCTGC 2 cut(s) 613, 1375
BspPI GGATC 2 cut(s) 349, 837
BspQI GCTCTTC 3 cut(s) 180, 592, 1289
BspT104I TTCGAA 1 cut(s) 590
BspTI CTTAAG 1 cut(s) 719
BspTNI GGTCTC 2 cut(s) 207, 1218
BsrDI GCAATG 2 cut(s) 1062, 1386
BsrFI RCCGGY 1 cut(s) 1121
BsrI ACTGG 5 cut(s) 446, 512, 1241, 1300, 1508
BssAI RCCGGY 1 cut(s) 1121
BssECI CCNNGG 5 cut(s) 16, 133, 498, 870, 1481
BssMI GATC 4 cut(s) 341, 433, 612, 829
BssNI GRCGYC 1 cut(s) 1244
BssSI CACGAG 1 cut(s) 38
BssT1I CCWWGG 2 cut(s) 16, 498
Bst2BI CACGAG 1 cut(s) 38
Bst2UI CCWGG 1 cut(s) 914
Bst4CI ACNGT 3 cut(s) 58, 541, 1447
Bst6I CTCTTC 7 cut(s) 180, 453, 592, 1275, 1289, 1348, 1452
BstACI GRCGYC 1 cut(s) 1244
BstAFI CTTAAG 1 cut(s) 719
BstAPI GCANNNNNTGC 1 cut(s) 1390
BstBAI YACGTR 1 cut(s) 336
BstBI TTCGAA 1 cut(s) 590
BstC8I GCNNGC 1 cut(s) 1377
BstDEI CTNAG 2 cut(s) 639, 1106
BstF5I GGATG 4 cut(s) 909, 985, 1364, 1489
BstFNI CGCG 1 cut(s) 1184
BstHHI GCGC 1 cut(s) 527
BstKTI GATC 4 cut(s) 344, 436, 615, 832
BstMAI GTCTC 3 cut(s) 55, 207, 1218
BstMBI GATC 4 cut(s) 341, 433, 612, 829
BstMWI GCNNNNNNNGC 5 cut(s) 503, 810, 1164, 1381, 1390
BstNI CCWGG 1 cut(s) 914
BstNSI RCATGY 1 cut(s) 406
BstSCI CCNGG 2 cut(s) 912, 1363
BstSLI GKGCMC 1 cut(s) 967
BstUI CGCG 1 cut(s) 1184
BstV1I GCAGC 3 cut(s) 515, 1330, 1475
BstX2I RGATCY 1 cut(s) 829
BstXI CCANNNNNNTGG 1 cut(s) 61
BstYI RGATCY 1 cut(s) 829
Bsu36I CCTNAGG 1 cut(s) 1106
BsuI GTATCC 1 cut(s) 14
BsuRI GGCC 3 cut(s) 122, 307, 497
BtsCI GGATG 4 cut(s) 909, 985, 1364, 1489
BtsIMutI CAGTG 3 cut(s) 1234, 1424, 1515
BveI ACCTGC 2 cut(s) 613, 1375
Cac8I GCNNGC 1 cut(s) 1377
CciI TCATGA 2 cut(s) 283, 387
CfoI GCGC 1 cut(s) 527
Cfr10I RCCGGY 1 cut(s) 1121
Cfr13I GGNCC 5 cut(s) 64, 90, 306, 371, 1124
CseI GACGC 1 cut(s) 1252
Csp6I GTAC 2 cut(s) 337, 1049
CspAI ACCGGT 1 cut(s) 1121
CviAII CATG 5 cut(s) 73, 284, 388, 403, 1453
CviQI GTAC 2 cut(s) 337, 1049
DdeI CTNAG 2 cut(s) 639, 1106
DpnI GATC 4 cut(s) 343, 435, 614, 831
DpnII GATC 4 cut(s) 341, 433, 612, 829
DraIII CACNNNGTG 1 cut(s) 1240
Eam1104I CTCTTC 7 cut(s) 180, 453, 592, 1275, 1289, 1348, 1452
EarI CTCTTC 7 cut(s) 180, 453, 592, 1275, 1289, 1348, 1452
EciI GGCGGA 1 cut(s) 440
Eco130I CCWWGG 2 cut(s) 16, 498
Eco24I GRGCYC 1 cut(s) 1489
Eco31I GGTCTC 2 cut(s) 207, 1218
Eco47I GGWCC 4 cut(s) 64, 90, 371, 1124
Eco57I CTGAAG 1 cut(s) 879
Eco81I CCTNAGG 1 cut(s) 1106
EcoO109I RGGNCCY 1 cut(s) 306
EcoRII CCWGG 1 cut(s) 912
EcoT14I CCWWGG 2 cut(s) 16, 498
EcoT22I ATGCAT 1 cut(s) 404
EcoT38I GRGCYC 1 cut(s) 1489
ErhI CCWWGG 2 cut(s) 16, 498
FaeI CATG 5 cut(s) 76, 287, 391, 406, 1456
FaqI GGGAC 2 cut(s) 215, 1347
FatI CATG 5 cut(s) 72, 283, 387, 402, 1452
FauI CCCGC 1 cut(s) 1189
FauNDI CATATG 1 cut(s) 674
Fnu4HI GCNGC 5 cut(s) 504, 1168, 1319, 1407, 1464
FokI GGATG 4 cut(s) 896, 972, 1371, 1476
FriOI GRGCYC 1 cut(s) 1489
Fsp4HI GCNGC 5 cut(s) 504, 1168, 1319, 1407, 1464
FspBI CTAG 4 cut(s) 47, 968, 1046, 1515
GlaI GCGC 1 cut(s) 526
GluI GCNGC 5 cut(s) 504, 1168, 1319, 1407, 1464
HaeIII GGCC 3 cut(s) 122, 307, 497
HapII CCGG 3 cut(s) 1122, 1262, 1365
HgaI GACGC 1 cut(s) 1252
HhaI GCGC 1 cut(s) 527
Hin1I GRCGYC 1 cut(s) 1244
Hin1II CATG 5 cut(s) 76, 287, 391, 406, 1456
Hin6I GCGC 1 cut(s) 525
HinP1I GCGC 1 cut(s) 525
HincII GTYRAC 2 cut(s) 645, 1450
HindII GTYRAC 2 cut(s) 645, 1450
HinfI GANTC 3 cut(s) 208, 1055, 1142
HpaII CCGG 3 cut(s) 1122, 1262, 1365
HphI GGTGA 8 cut(s) 70, 158, 203, 553, 637, 827, 1106, 1252
Hpy166II GTNNAC 3 cut(s) 645, 1349, 1450
Hpy188III TCNNGA 8 cut(s) 284, 388, 561, 616, 952, 989, 1135, 1539
Hpy8I GTNNAC 3 cut(s) 645, 1349, 1450
HpyAV CCTTC 7 cut(s) 113, 417, 452, 787, 800, 1082, 1433
HpyCH4III ACNGT 3 cut(s) 58, 541, 1447
HpyCH4IV ACGT 2 cut(s) 335, 552
HpyF10VI GCNNNNNNNGC 5 cut(s) 503, 810, 1164, 1381, 1390
HpyF3I CTNAG 2 cut(s) 639, 1106
HpySE526I ACGT 2 cut(s) 335, 552
Hsp92I GRCGYC 1 cut(s) 1244
Hsp92II CATG 5 cut(s) 76, 287, 391, 406, 1456
HspAI GCGC 1 cut(s) 525
Kzo9I GATC 4 cut(s) 341, 433, 612, 829
LguI GCTCTTC 3 cut(s) 180, 592, 1289
LmnI GCTCC 4 cut(s) 160, 367, 444, 769
Lsp1109I GCAGC 3 cut(s) 515, 1330, 1475
LweI GCATC 2 cut(s) 808, 987
MaeI CTAG 4 cut(s) 47, 968, 1046, 1515
MaeII ACGT 2 cut(s) 335, 552
MaeIII GTNAC 4 cut(s) 541, 731, 1240, 1273
MalI GATC 4 cut(s) 343, 435, 614, 831
MboI GATC 4 cut(s) 341, 433, 612, 829
MflI RGATCY 1 cut(s) 829
MhlI GDGCHC 3 cut(s) 774, 967, 1489
MluCI AATT 6 cut(s) 83, 658, 688, 1017, 1029, 1151
MlyI GAGTC 1 cut(s) 1151
MmeI TCCRAC 2 cut(s) 213, 324
Mph1103I ATGCAT 1 cut(s) 404
MroXI GAANNNNTTC 1 cut(s) 269
MseI TTAA 5 cut(s) 657, 720, 825, 1130, 1472
MslI CAYNNNNRTG 1 cut(s) 990
MspA1I CMGCKG 2 cut(s) 347, 1466
MspCI CTTAAG 1 cut(s) 719
MspI CCGG 3 cut(s) 1122, 1262, 1365
MspR9I CCNGG 2 cut(s) 914, 1365
Mva1269I GAATGC 1 cut(s) 905
MvaI CCWGG 1 cut(s) 914
MvnI CGCG 1 cut(s) 1184
MwoI GCNNNNNNNGC 5 cut(s) 503, 810, 1164, 1381, 1390
NciI CCSGG 1 cut(s) 1365
NdeI CATATG 1 cut(s) 674
NdeII GATC 4 cut(s) 341, 433, 612, 829
NlaIII CATG 5 cut(s) 76, 287, 391, 406, 1456
NlaIV GGNNCC 3 cut(s) 226, 934, 1369
NmeAIII GCCGAG 1 cut(s) 532
NmuCI GTSAC 4 cut(s) 541, 731, 1240, 1273
NsiI ATGCAT 1 cut(s) 404
NspI RCATGY 1 cut(s) 406
NspV TTCGAA 1 cut(s) 590
PagI TCATGA 2 cut(s) 283, 387
PaqCI CACCTGC 1 cut(s) 613
PciSI GCTCTTC 3 cut(s) 180, 592, 1289
PctI GAATGC 1 cut(s) 905
PdmI GAANNNNTTC 1 cut(s) 269
PfeI GAWTC 2 cut(s) 208, 1055
Pfl23II CGTACG 1 cut(s) 336
PflMI CCANNNNNTGG 2 cut(s) 87, 1236
PfoI TCCNGGA 2 cut(s) 912, 1363
PinAI ACCGGT 1 cut(s) 1121
PkrI GCNGC 5 cut(s) 505, 1169, 1320, 1408, 1465
PleI GAGTC 1 cut(s) 1150
PpsI GAGTC 1 cut(s) 1150
Ppu21I YACGTR 1 cut(s) 336
PshBI ATTAAT 1 cut(s) 1130
Psp6I CCWGG 1 cut(s) 912
PspGI CCWGG 1 cut(s) 912
PspLI CGTACG 1 cut(s) 336
PspN4I GGNNCC 3 cut(s) 226, 934, 1369
PspPI GGNCC 5 cut(s) 64, 90, 306, 371, 1124
PsuI RGATCY 1 cut(s) 829
PvuII CAGCTG 2 cut(s) 347, 1466
RsaI GTAC 2 cut(s) 338, 1050
RsaNI GTAC 2 cut(s) 337, 1049
RseI CAYNNNNRTG 1 cut(s) 990
SapI GCTCTTC 3 cut(s) 180, 592, 1289
SaqAI TTAA 5 cut(s) 657, 720, 825, 1130, 1472
SatI GCNGC 5 cut(s) 504, 1168, 1319, 1407, 1464
Sau3AI GATC 4 cut(s) 341, 433, 612, 829
Sau96I GGNCC 5 cut(s) 64, 90, 306, 371, 1124
SchI GAGTC 1 cut(s) 1151
ScrFI CCNGG 2 cut(s) 914, 1365
SduI GDGCHC 3 cut(s) 774, 967, 1489
SfaNI GCATC 2 cut(s) 808, 987
SfuI TTCGAA 1 cut(s) 590
SinI GGWCC 4 cut(s) 64, 90, 371, 1124
SmiMI CAYNNNNRTG 1 cut(s) 990
SmlI CTYRAG 1 cut(s) 719
SmoI CTYRAG 1 cut(s) 719
Sse9I AATT 6 cut(s) 83, 658, 688, 1017, 1029, 1151
SsiI CCGC 5 cut(s) 451, 813, 1167, 1182, 1407
SspMI CTAG 4 cut(s) 47, 968, 1046, 1515
StyD4I CCNGG 2 cut(s) 912, 1363
StyI CCWWGG 2 cut(s) 16, 498
TaaI ACNGT 3 cut(s) 58, 541, 1447
TaiI ACGT 2 cut(s) 338, 555
TaqI TCGA 4 cut(s) 432, 560, 590, 849
TaqII GACCGA 1 cut(s) 1292
TasI AATT 6 cut(s) 83, 658, 688, 1017, 1029, 1151
TatI WGTACW 1 cut(s) 1048
TauI GCSGC 2 cut(s) 1170, 1409
TfiI GAWTC 2 cut(s) 208, 1055
Tru1I TTAA 5 cut(s) 657, 720, 825, 1130, 1472
Tru9I TTAA 5 cut(s) 657, 720, 825, 1130, 1472
TscAI CASTG 3 cut(s) 1241, 1424, 1515
TseFI GTSAC 4 cut(s) 541, 731, 1240, 1273
TseI GCWGC 3 cut(s) 503, 1318, 1463
Tsp45I GTSAC 4 cut(s) 541, 731, 1240, 1273
TspDTI ATGAA 7 cut(s) 345, 376, 404, 661, 879, 898, 1316
TspGWI ACGGA 2 cut(s) 217, 354
TspRI CASTG 3 cut(s) 1241, 1424, 1515
Van91I CCANNNNNTGG 2 cut(s) 87, 1236
Vha464I CTTAAG 1 cut(s) 719
VpaK11BI GGWCC 4 cut(s) 64, 90, 371, 1124
VspI ATTAAT 1 cut(s) 1130
XapI RAATTY 3 cut(s) 83, 688, 1151
XceI RCATGY 1 cut(s) 406
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
XmnI GAANNNNTTC 1 cut(s) 269
XspI CTAG 4 cut(s) 47, 968, 1046, 1515
Zsp2I ATGCAT 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.