Rroxscaffold_6G00401350

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004503
Physical Location & Seq
Forward (+)
23693632 .. 23695863
2232 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00401350.1

Sequence Viewer

Length: 1431 bp
ATGGCCTTACCAATCATTGGACACCTTCACCTCCTTGCCCCAATACCTCACCAAGCTCTTCACAAGCTATCAAACCGCCTTGGACCTTTAGTTCGCATCTTTCTTGGATCTGTCCCTTGTGTAGTTGTTTCCTCCCCGGAAATGGCGAAAGAGTTCCTCAAGACTCATGAAGCTTCCTTCTCCAACAGGCCACACATGGCTGCTGTTGACTATCTCACATATGGGTCAGCTGATTTTTCGTTTGCACCTTATGGACCCTACTGGAAATTCATGAAGAAACTTTGCATGTCTGAACTTCTCGGTGGAAGAACACTTGATCAGCTCCTTCCAGTGAGGCGTGAAGAGATGACGAGCTTCATAGGTTTGATGCTAAACAAGGCCAAGGCAAATGAGGCAGTTGATCTTGGAGCTGAGCTTATGAATGTAACGAACAATATCATATCGAGAATGCTTATGGGGCAGAGTTGTTCTGCGGACGAAAAAGAGGCTGATGAGGTCAGGAAGTTGGTCAAAGCTGTTGCCGAGCTTACAGGGAAGTTCAATTTGTCAGACTTCATTTGGTTTTGTAAGAACTTGGATTTGCAGGGATTTGGAAAAAGGCTTAAGGAAGTGCGTGATCAGTTCGACACGATGATGCAGAGGATCATAAAGGAGCACCAAGAGGCAAGAAAGAAGAAGAAGGAAGTAGGTGAAAATGATGATGATGTTAAAGATTTGCTTGACATTTTACTTGATATATCTGAAGATGAGACCTCAGAGATGAGATTGACTAGAGAAAACATAAAGGCCTTCATCCTGGACATATTTGCTGCTGGTACCGACACATCTGCAATTACAACAGAATGGGCACTAGCAGAGCTAATCAACCACCCAGATGTGATGAACAAAGCAAGACAAGAAATCGACACCATAATCGGGAACAACAGACTAGTAAAAGAATCAGACATTGCAAACCTTCCTTACCTGCAAGCTATAGTGAAAGAGACACTAAGACTTCATCCAACAGGTCCATTAATTGTGAGAGAGTCAACTGAACCCTGCTCAATTGGGGGCTATGAGATTCCAGCAAAAACCAGATTATTTGTAAACGTGTGGGCTGTCAACAGAGACCCCAATCACTGGGAGAAGCCTCTGGAGTTTGAGCCAGAAAGGTTTACACACAATCAAGGTAATGGGAAGAGCCAACTAGATGTAAGAGGACAACATTTTCATTTGTTGCCATTTGGTAGTGGAAGAAGAAGTTGTCCTGGGACCTCACTTGCACTGCAGGTTGTGCAGACAATGCTCGCAGCTATGATTCAGTGCTTTGAATGGAAGGTATCTAATGGTACTGTTGACATGGAAGAGGGCCCTGGATTAACACTTCCAAGGGCTCATCCCTTGGTTTGTGTTCCAGTTGCCAGGATCAATCCCTTTCCATCTTTCGAATAA

Protein Analysis

476

Amino Acids

53.56

Weight (kDa)

6.1

Isoelectric Point (pI)

30.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 3 - 463 2.6e-110 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 972, 1258
Acc65I GGTACC 1 cut(s) 815
AccB1I GGYRCC 1 cut(s) 815
AccB7I CCANNNNNTGG 2 cut(s) 17, 1119
AciI CCGC 2 cut(s) 76, 473
AclWI GGATC 3 cut(s) 115, 650, 1412
AcsI RAATTY 1 cut(s) 266
AcuI CTGAAG 1 cut(s) 762
AfaI GTAC 2 cut(s) 817, 1330
AfiI CCNNNNNNNGG 4 cut(s) 17, 142, 915, 1119
AflII CTTAAG 1 cut(s) 602
AflIII ACRYGT 1 cut(s) 1089
AgsI TTSAA 2 cut(s) 541, 1310
AhlI ACTAGT 1 cut(s) 928
AjnI CCWGG 4 cut(s) 795, 1246, 1351, 1400
AloI GAACNNNNNNTCC 2 cut(s) 75, 107
Alw21I GWGCWC 1 cut(s) 657
Alw26I GTCTC 3 cut(s) 743, 977, 1101
AlwI GGATC 3 cut(s) 115, 650, 1412
AoxI GGCC 5 cut(s) 3, 188, 378, 786, 1348
ApaI GGGCCC 1 cut(s) 1352
ApeKI GCWGC 3 cut(s) 200, 809, 1289
ApoI RAATTY 1 cut(s) 266
ArsI GACNNNNNNTTYG 2 cut(s) 75, 107
AseI ATTAAT 1 cut(s) 1013
Asp700I GAANNNNTTC 1 cut(s) 152
Asp718I GGTACC 1 cut(s) 815
AspS9I GGNCC 6 cut(s) 83, 254, 1007, 1251, 1348, 1349
AsuC2I CCSGG 1 cut(s) 137
AsuHPI GGTGA 3 cut(s) 20, 41, 701
AsuII TTCGAA 1 cut(s) 1425
AvaII GGWCC 4 cut(s) 83, 254, 1007, 1251
BaeGI GKGCMC 2 cut(s) 850, 1352
BanI GGYRCC 1 cut(s) 815
BanII GRGCYC 2 cut(s) 1352, 1375
Bbv12I GWGCWC 1 cut(s) 657
BbvI GCAGC 3 cut(s) 187, 796, 1301
BccI CCATC 1 cut(s) 1426
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BciT130I CCWGG 4 cut(s) 797, 1248, 1353, 1402
BclI TGATCA 2 cut(s) 316, 616
BcnI CCSGG 1 cut(s) 137
BcoDI GTCTC 3 cut(s) 743, 977, 1101
BcuI ACTAGT 1 cut(s) 928
BfaI CTAG 4 cut(s) 771, 851, 929, 1187
BfmI CTRYAG 2 cut(s) 972, 1265
BfrI CTTAAG 1 cut(s) 602
BfuAI ACCTGC 2 cut(s) 972, 1258
BisI GCNGC 3 cut(s) 201, 810, 1290
BlpI GCTNAGC 1 cut(s) 411
BlsI GCNGC 3 cut(s) 202, 811, 1291
Bme1390I CCNGG 5 cut(s) 137, 797, 1248, 1353, 1402
Bme18I GGWCC 4 cut(s) 83, 254, 1007, 1251
BmgT120I GGNCC 6 cut(s) 83, 254, 1007, 1251, 1348, 1349
BmiI GGNNCC 4 cut(s) 256, 817, 1252, 1350
BmrFI CCNGG 5 cut(s) 137, 797, 1248, 1353, 1402
BmrI ACTGGG 1 cut(s) 1129
BmsI GCATC 3 cut(s) 105, 357, 624
BmuI ACTGGG 1 cut(s) 1129
BpmI CTGGAG 1 cut(s) 1154
Bpu1102I GCTNAGC 1 cut(s) 411
Bpu14I TTCGAA 1 cut(s) 1425
BpuEI CTTGAG 1 cut(s) 143
BpuMI CCSGG 1 cut(s) 137
BsaI GGTCTC 2 cut(s) 743, 1101
BsaJI CCNNGG 7 cut(s) 79, 135, 381, 1247, 1351, 1367, 1380
Bsc4I CCNNNNNNNGG 4 cut(s) 17, 142, 915, 1119
Bse1I ACTGG 4 cut(s) 266, 329, 1124, 1394
Bse3DI GCAATG 1 cut(s) 945
BseBI CCWGG 4 cut(s) 797, 1248, 1353, 1402
BseDI CCNNGG 7 cut(s) 79, 135, 381, 1247, 1351, 1367, 1380
BseGI GGATG 3 cut(s) 792, 997, 1375
BseLI CCNNNNNNNGG 4 cut(s) 17, 142, 915, 1119
BseMI GCAATG 1 cut(s) 945
BseMII CTCAG 2 cut(s) 402, 768
BseNI ACTGG 4 cut(s) 266, 329, 1124, 1394
BseSI GKGCMC 2 cut(s) 850, 1352
BseXI GCAGC 3 cut(s) 187, 796, 1301
BsgI GTGCAG 1 cut(s) 1295
BshFI GGCC 5 cut(s) 5, 190, 380, 788, 1350
BshNI GGYRCC 1 cut(s) 815
BsiHKAI GWGCWC 1 cut(s) 657
BsiSI CCGG 1 cut(s) 137
BslFI GGGAC 2 cut(s) 98, 1264
BslI CCNNNNNNNGG 4 cut(s) 17, 142, 915, 1119
BsmAI GTCTC 3 cut(s) 743, 977, 1101
BsmFI GGGAC 2 cut(s) 98, 1264
BsmI GAATGC 1 cut(s) 453
BsnI GGCC 5 cut(s) 5, 190, 380, 788, 1350
Bso31I GGTCTC 2 cut(s) 743, 1101
Bsp119I TTCGAA 1 cut(s) 1425
Bsp120I GGGCCC 1 cut(s) 1348
Bsp1286I GDGCHC 4 cut(s) 657, 850, 1352, 1375
Bsp143I GATC 6 cut(s) 107, 316, 400, 616, 642, 1404
Bsp1720I GCTNAGC 1 cut(s) 411
BspACI CCGC 2 cut(s) 76, 473
BspANI GGCC 5 cut(s) 5, 190, 380, 788, 1350
BspCNI CTCAG 2 cut(s) 403, 767
BspHI TCATGA 2 cut(s) 166, 270
BspLI GGNNCC 4 cut(s) 256, 817, 1252, 1350
BspMAI CTGCAG 1 cut(s) 1269
BspMI ACCTGC 2 cut(s) 972, 1258
BspPI GGATC 3 cut(s) 115, 650, 1412
BspQI GCTCTTC 2 cut(s) 63, 1172
BspT104I TTCGAA 1 cut(s) 1425
BspT107I GGYRCC 1 cut(s) 815
BspTI CTTAAG 1 cut(s) 602
BspTNI GGTCTC 2 cut(s) 743, 1101
BsrDI GCAATG 1 cut(s) 945
BsrI ACTGG 4 cut(s) 266, 329, 1124, 1394
BssECI CCNNGG 7 cut(s) 79, 135, 381, 1247, 1351, 1367, 1380
BssMI GATC 6 cut(s) 107, 316, 400, 616, 642, 1404
BssT1I CCWWGG 4 cut(s) 79, 381, 1367, 1380
Bst2UI CCWGG 4 cut(s) 797, 1248, 1353, 1402
Bst4CI ACNGT 1 cut(s) 1333
Bst6I CTCTTC 4 cut(s) 63, 336, 1172, 1338
BstAFI CTTAAG 1 cut(s) 602
BstAPI GCANNNNNTGC 2 cut(s) 1273, 1282
BstBI TTCGAA 1 cut(s) 1425
BstC8I GCNNGC 2 cut(s) 969, 1287
BstDEI CTNAG 3 cut(s) 411, 754, 989
BstF5I GGATG 3 cut(s) 792, 997, 1375
BstKTI GATC 6 cut(s) 110, 319, 403, 619, 645, 1407
BstMAI GTCTC 3 cut(s) 743, 977, 1101
BstMBI GATC 6 cut(s) 107, 316, 400, 616, 642, 1404
BstMWI GCNNNNNNNGC 4 cut(s) 392, 457, 1273, 1282
BstNI CCWGG 4 cut(s) 797, 1248, 1353, 1402
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 5 cut(s) 135, 795, 1246, 1351, 1400
BstSFI CTRYAG 2 cut(s) 972, 1265
BstSLI GKGCMC 2 cut(s) 850, 1352
BstV1I GCAGC 3 cut(s) 187, 796, 1301
BstX2I RGATCY 1 cut(s) 107
BstYI RGATCY 1 cut(s) 107
BsuRI GGCC 5 cut(s) 5, 190, 380, 788, 1350
BtsCI GGATG 3 cut(s) 792, 997, 1375
BtsI GCAGTG 1 cut(s) 1262
BtsIMutI CAGTG 4 cut(s) 336, 1117, 1262, 1307
BveI ACCTGC 2 cut(s) 972, 1258
Cac8I GCNNGC 2 cut(s) 969, 1287
CciI TCATGA 2 cut(s) 166, 270
Cfr13I GGNCC 6 cut(s) 83, 254, 1007, 1251, 1348, 1349
Csp6I GTAC 2 cut(s) 816, 1329
CviAII CATG 5 cut(s) 167, 196, 271, 286, 1339
CviQI GTAC 2 cut(s) 816, 1329
DdeI CTNAG 3 cut(s) 411, 754, 989
DpnI GATC 6 cut(s) 109, 318, 402, 618, 644, 1406
DpnII GATC 6 cut(s) 107, 316, 400, 616, 642, 1404
Eam1104I CTCTTC 4 cut(s) 63, 336, 1172, 1338
EarI CTCTTC 4 cut(s) 63, 336, 1172, 1338
Eco130I CCWWGG 4 cut(s) 79, 381, 1367, 1380
Eco147I AGGCCT 1 cut(s) 788
Eco24I GRGCYC 2 cut(s) 1352, 1375
Eco31I GGTCTC 2 cut(s) 743, 1101
Eco47I GGWCC 4 cut(s) 83, 254, 1007, 1251
Eco57I CTGAAG 1 cut(s) 762
EcoO109I RGGNCCY 3 cut(s) 1251, 1348, 1349
EcoRII CCWGG 4 cut(s) 795, 1246, 1351, 1400
EcoT14I CCWWGG 4 cut(s) 79, 381, 1367, 1380
EcoT38I GRGCYC 2 cut(s) 1352, 1375
ErhI CCWWGG 4 cut(s) 79, 381, 1367, 1380
FaeI CATG 5 cut(s) 170, 199, 274, 289, 1342
FalI AAGNNNNNCTT 2 cut(s) 702, 734
FaqI GGGAC 2 cut(s) 98, 1264
FatI CATG 5 cut(s) 166, 195, 270, 285, 1338
FauNDI CATATG 1 cut(s) 220
FbaI TGATCA 2 cut(s) 316, 616
Fnu4HI GCNGC 3 cut(s) 201, 810, 1290
FokI GGATG 3 cut(s) 779, 984, 1362
FriOI GRGCYC 2 cut(s) 1352, 1375
Fsp4HI GCNGC 3 cut(s) 201, 810, 1290
FspBI CTAG 4 cut(s) 771, 851, 929, 1187
GluI GCNGC 3 cut(s) 201, 810, 1290
GsuI CTGGAG 1 cut(s) 1154
HaeIII GGCC 5 cut(s) 5, 190, 380, 788, 1350
HapII CCGG 1 cut(s) 137
Hin1II CATG 5 cut(s) 170, 199, 274, 289, 1342
HincII GTYRAC 4 cut(s) 208, 1029, 1102, 1336
HindII GTYRAC 4 cut(s) 208, 1029, 1102, 1336
HindIII AAGCTT 1 cut(s) 171
HinfI GANTC 5 cut(s) 163, 938, 1025, 1060, 1297
HpaII CCGG 1 cut(s) 137
HphI GGTGA 3 cut(s) 20, 41, 701
Hpy166II GTNNAC 6 cut(s) 208, 1029, 1087, 1102, 1155, 1336
Hpy188I TCNGA 5 cut(s) 292, 550, 742, 757, 943
Hpy188III TCNNGA 7 cut(s) 160, 167, 271, 444, 499, 916, 1133
Hpy8I GTNNAC 6 cut(s) 208, 1029, 1087, 1102, 1155, 1336
HpyAV CCTTC 7 cut(s) 35, 187, 335, 673, 799, 965, 1309
HpyCH4III ACNGT 1 cut(s) 1333
HpyCH4IV ACGT 1 cut(s) 1089
HpyF10VI GCNNNNNNNGC 4 cut(s) 392, 457, 1273, 1282
HpyF3I CTNAG 3 cut(s) 411, 754, 989
HpySE526I ACGT 1 cut(s) 1089
Hsp92II CATG 5 cut(s) 170, 199, 274, 289, 1342
KpnI GGTACC 1 cut(s) 819
Ksp22I TGATCA 2 cut(s) 316, 616
Kzo9I GATC 6 cut(s) 107, 316, 400, 616, 642, 1404
LguI GCTCTTC 2 cut(s) 63, 1172
LmnI GCTCC 3 cut(s) 327, 407, 652
Lsp1109I GCAGC 3 cut(s) 187, 796, 1301
LweI GCATC 3 cut(s) 105, 357, 624
MaeI CTAG 4 cut(s) 771, 851, 929, 1187
MaeII ACGT 1 cut(s) 1089
MaeIII GTNAC 1 cut(s) 424
MalI GATC 6 cut(s) 109, 318, 402, 618, 644, 1406
MboI GATC 6 cut(s) 107, 316, 400, 616, 642, 1404
MfeI CAATTG 1 cut(s) 1044
MflI RGATCY 1 cut(s) 107
MhlI GDGCHC 4 cut(s) 657, 850, 1352, 1375
MluCI AATT 5 cut(s) 266, 541, 831, 1014, 1044
MlyI GAGTC 2 cut(s) 157, 1034
MmeI TCCRAC 2 cut(s) 207, 1025
MroXI GAANNNNTTC 1 cut(s) 152
MseI TTAA 4 cut(s) 603, 708, 1013, 1358
MslI CAYNNNNRTG 2 cut(s) 632, 873
MspA1I CMGCKG 1 cut(s) 230
MspCI CTTAAG 1 cut(s) 602
MspI CCGG 1 cut(s) 137
MspR9I CCNGG 5 cut(s) 137, 797, 1248, 1353, 1402
MunI CAATTG 1 cut(s) 1044
Mva1269I GAATGC 1 cut(s) 453
MvaI CCWGG 4 cut(s) 797, 1248, 1353, 1402
MwoI GCNNNNNNNGC 4 cut(s) 392, 457, 1273, 1282
NciI CCSGG 1 cut(s) 137
NdeI CATATG 1 cut(s) 220
NdeII GATC 6 cut(s) 107, 316, 400, 616, 642, 1404
NlaIII CATG 5 cut(s) 170, 199, 274, 289, 1342
NlaIV GGNNCC 4 cut(s) 256, 817, 1252, 1350
NmeAIII GCCGAG 1 cut(s) 547
NspI RCATGY 1 cut(s) 289
NspV TTCGAA 1 cut(s) 1425
PagI TCATGA 2 cut(s) 166, 270
PceI AGGCCT 1 cut(s) 788
PciSI GCTCTTC 2 cut(s) 63, 1172
PctI GAATGC 1 cut(s) 453
PdmI GAANNNNTTC 1 cut(s) 152
PfeI GAWTC 3 cut(s) 938, 1060, 1297
PflMI CCANNNNNTGG 2 cut(s) 17, 1119
PfoI TCCNGGA 1 cut(s) 795
PkrI GCNGC 3 cut(s) 202, 811, 1291
PleI GAGTC 2 cut(s) 157, 1033
PpsI GAGTC 2 cut(s) 157, 1033
PpuMI RGGWCCY 1 cut(s) 1251
PshBI ATTAAT 1 cut(s) 1013
Psp5II RGGWCCY 1 cut(s) 1251
Psp6I CCWGG 4 cut(s) 795, 1246, 1351, 1400
PspGI CCWGG 4 cut(s) 795, 1246, 1351, 1400
PspN4I GGNNCC 4 cut(s) 256, 817, 1252, 1350
PspOMI GGGCCC 1 cut(s) 1348
PspPI GGNCC 6 cut(s) 83, 254, 1007, 1251, 1348, 1349
PspPPI RGGWCCY 1 cut(s) 1251
PstI CTGCAG 1 cut(s) 1269
PsuI RGATCY 1 cut(s) 107
PvuII CAGCTG 1 cut(s) 230
RsaI GTAC 2 cut(s) 817, 1330
RsaNI GTAC 2 cut(s) 816, 1329
RseI CAYNNNNRTG 2 cut(s) 632, 873
SapI GCTCTTC 2 cut(s) 63, 1172
SaqAI TTAA 4 cut(s) 603, 708, 1013, 1358
SatI GCNGC 3 cut(s) 201, 810, 1290
Sau3AI GATC 6 cut(s) 107, 316, 400, 616, 642, 1404
Sau96I GGNCC 6 cut(s) 83, 254, 1007, 1251, 1348, 1349
SchI GAGTC 2 cut(s) 157, 1034
ScrFI CCNGG 5 cut(s) 137, 797, 1248, 1353, 1402
SduI GDGCHC 4 cut(s) 657, 850, 1352, 1375
SfaNI GCATC 3 cut(s) 105, 357, 624
SfcI CTRYAG 2 cut(s) 972, 1265
SfuI TTCGAA 1 cut(s) 1425
SinI GGWCC 4 cut(s) 83, 254, 1007, 1251
SmiMI CAYNNNNRTG 2 cut(s) 632, 873
SmlI CTYRAG 2 cut(s) 158, 602
SmoI CTYRAG 2 cut(s) 158, 602
SpeI ACTAGT 1 cut(s) 928
Sse9I AATT 5 cut(s) 266, 541, 831, 1014, 1044
SseBI AGGCCT 1 cut(s) 788
SsiI CCGC 2 cut(s) 76, 473
SspMI CTAG 4 cut(s) 771, 851, 929, 1187
StuI AGGCCT 1 cut(s) 788
StyD4I CCNGG 5 cut(s) 135, 795, 1246, 1351, 1400
StyI CCWWGG 4 cut(s) 79, 381, 1367, 1380
TaaI ACNGT 1 cut(s) 1333
TaiI ACGT 1 cut(s) 1092
TaqI TCGA 4 cut(s) 443, 624, 903, 1425
TasI AATT 5 cut(s) 266, 541, 831, 1014, 1044
TfiI GAWTC 3 cut(s) 938, 1060, 1297
Tru1I TTAA 4 cut(s) 603, 708, 1013, 1358
Tru9I TTAA 4 cut(s) 603, 708, 1013, 1358
TscAI CASTG 4 cut(s) 336, 1124, 1269, 1307
TseI GCWGC 3 cut(s) 200, 809, 1289
TspRI CASTG 4 cut(s) 336, 1124, 1269, 1307
Van91I CCANNNNNTGG 2 cut(s) 17, 1119
Vha464I CTTAAG 1 cut(s) 602
VpaK11BI GGWCC 4 cut(s) 83, 254, 1007, 1251
VspI ATTAAT 1 cut(s) 1013
XapI RAATTY 1 cut(s) 266
XceI RCATGY 1 cut(s) 289
XmnI GAANNNNTTC 1 cut(s) 152
XspI CTAG 4 cut(s) 771, 851, 929, 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.