pycom14g06650

carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012107
Physical Location & Seq
Reverse (-)
6661976 .. 6663469
1494 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 633 bp
ATGCAATCTCCAATTGATCTTCTTGCCCAAAGGGTGCAGGTTTTCCCTAAATTGGGGAAACTGAAAAGGGAATACAAACGAGCTCCTGCTATTGTGAAGAACCGAGGTCATGACATCACGATGAGGTGGAATGGAGATGCAGGAAAAATCAATATAAATGGGACTGACTATAGACTGCTACAATGTCATTGGCATTCACCCTCCGAACACACATTTAACGGATCAAGGTATGCTTTGGAGTTTCATATCGTTCATGTAAGCTCGACCAGAAAGATTGCTGTTATTGGAATTGTGTACAAATATGGTCGACCTGATCCCTTCCTTTCAAAGCTGTTCCACCACATAAAATCAGTTGGAAAAGAAGAGGTTGACATAGGGATTATTAATCCTGGAGATATTAAGTTCGGAAGCAGAAAGTACTACAGATATATCGGTTCTCTTACCGTTCCTCCATGCACTGAGGGTGTAATTTGGACAATCGTCAAAAAGGTCAGGACAGTTTCAAGGGAGCAAGTGCACGCACTACGGGAGGCGGTTCATGATGGATATGAGGCGAATGCAAGGCCAACTCAAGAACTGGATGGAAGACCAGTGTCGTTATACACTCCAAGGAATATTGGAGGTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase LSM

Protein Analysis

211

Amino Acids

23.83

Weight (kDa)

9.83

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 2 - 199 6.5e-38 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012107)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20990
fragaria_vesca FvH4_6g24590 FvH4_6g24590 FvH4_6g24590
malus_domestica MD12G1083000.v1.1 MD14G1078100.v1.1
prunus_persica Prupe.7G052800_v2.0.a1
pyrus_communis pycom12g06990 pycom14g06650
rosa_chinensis RchiOBHm_Chr3g0483901
rosa_laevigata RLG00000023253
rosa_multiflora Rmu_sc0002252.1_g000013
rosa_roxburghii Rroxscaffold_6G00398060
rosa_rugosa Rorug03G0208600
rosa_samantha Rh3AG257300 Rh3BG293700 Rh3CG292000 Rh3DG287100
rosa_wichuraiana Rw3G023260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 28
AccI GTMKAC 1 cut(s) 307
AciI CCGC 1 cut(s) 533
AclWI GGATC 2 cut(s) 229, 308
AfaI GTAC 2 cut(s) 296, 419
AfiI CCNNNNNNNGG 2 cut(s) 52, 53
AgsI TTSAA 2 cut(s) 327, 504
AjnI CCWGG 1 cut(s) 388
AluBI AGCT 3 cut(s) 83, 261, 331
AluI AGCT 3 cut(s) 83, 261, 331
Alw21I GWGCWC 2 cut(s) 85, 519
Alw44I GTGCAC 1 cut(s) 515
AlwI GGATC 2 cut(s) 229, 308
AoxI GGCC 1 cut(s) 563
ApaLI GTGCAC 1 cut(s) 515
AseI ATTAAT 1 cut(s) 384
AsuHPI GGTGA 1 cut(s) 189
BaeGI GKGCMC 1 cut(s) 519
BanII GRGCYC 1 cut(s) 85
BbsI GAAGAC 1 cut(s) 592
Bbv12I GWGCWC 2 cut(s) 85, 519
BccI CCATC 2 cut(s) 536, 575
BciT130I CCWGG 1 cut(s) 390
BfmI CTRYAG 2 cut(s) 169, 421
BfuAI ACCTGC 1 cut(s) 28
BmcAI AGTACT 1 cut(s) 419
Bme1390I CCNGG 1 cut(s) 390
BmrFI CCNGG 1 cut(s) 390
BmsI GCATC 1 cut(s) 127
BoxI GACNNNNGTC 2 cut(s) 479, 592
BpiI GAAGAC 1 cut(s) 592
BpmI CTGGAG 1 cut(s) 411
BpuEI CTTGAG 1 cut(s) 555
BsaJI CCNNGG 2 cut(s) 103, 608
BsaXI ACNNNNNCTCC 2 cut(s) 433, 463
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 53
Bse1I ACTGG 2 cut(s) 582, 590
BseBI CCWGG 1 cut(s) 390
BseDI CCNNGG 2 cut(s) 103, 608
BseGI GGATG 1 cut(s) 586
BseLI CCNNNNNNNGG 2 cut(s) 52, 53
BseMII CTCAG 1 cut(s) 450
BseNI ACTGG 2 cut(s) 582, 590
BseSI GKGCMC 1 cut(s) 519
BsgI GTGCAG 1 cut(s) 56
BshFI GGCC 1 cut(s) 565
BsiHKAI GWGCWC 2 cut(s) 85, 519
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 2 cut(s) 52, 53
BsmFI GGGAC 1 cut(s) 175
BsmI GAATGC 2 cut(s) 193, 562
BsnI GGCC 1 cut(s) 565
Bsp1286I GDGCHC 2 cut(s) 85, 519
Bsp1407I TGTACA 1 cut(s) 294
Bsp143I GATC 3 cut(s) 16, 221, 313
BspACI CCGC 1 cut(s) 533
BspANI GGCC 1 cut(s) 565
BspCNI CTCAG 1 cut(s) 451
BspHI TCATGA 2 cut(s) 109, 538
BspMI ACCTGC 1 cut(s) 28
BspPI GGATC 2 cut(s) 229, 308
BsrGI TGTACA 1 cut(s) 294
BsrI ACTGG 2 cut(s) 582, 590
BssECI CCNNGG 2 cut(s) 103, 608
BssMI GATC 3 cut(s) 16, 221, 313
BssT1I CCWWGG 1 cut(s) 608
Bst2UI CCWGG 1 cut(s) 390
Bst4CI ACNGT 2 cut(s) 445, 499
Bst6I CTCTTC 1 cut(s) 357
BstAUI TGTACA 1 cut(s) 294
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 1 cut(s) 459
BstF5I GGATG 1 cut(s) 586
BstKTI GATC 3 cut(s) 19, 224, 316
BstMBI GATC 3 cut(s) 16, 221, 313
BstNI CCWGG 1 cut(s) 390
BstPAI GACNNNNGTC 2 cut(s) 479, 592
BstSCI CCNGG 1 cut(s) 388
BstSFI CTRYAG 2 cut(s) 169, 421
BstSLI GKGCMC 1 cut(s) 519
BstV2I GAAGAC 1 cut(s) 592
BsuRI GGCC 1 cut(s) 565
BtsCI GGATG 1 cut(s) 586
BtsIMutI CAGTG 2 cut(s) 456, 597
BveI ACCTGC 1 cut(s) 28
Cac8I GCNNGC 1 cut(s) 519
CciI TCATGA 2 cut(s) 109, 538
Csp6I GTAC 2 cut(s) 295, 418
CviAII CATG 4 cut(s) 110, 254, 453, 539
CviJI RGCY 4 cut(s) 83, 261, 331, 565
CviKI_1 RGCY 4 cut(s) 83, 261, 331, 565
CviQI GTAC 2 cut(s) 295, 418
DdeI CTNAG 1 cut(s) 459
DpnI GATC 3 cut(s) 18, 223, 315
DpnII GATC 3 cut(s) 16, 221, 313
Eam1104I CTCTTC 1 cut(s) 357
EarI CTCTTC 1 cut(s) 357
Ecl136II GAGCTC 1 cut(s) 83
Eco130I CCWWGG 1 cut(s) 608
Eco24I GRGCYC 1 cut(s) 85
Eco53kI GAGCTC 1 cut(s) 83
EcoICRI GAGCTC 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 388
EcoT14I CCWWGG 1 cut(s) 608
EcoT38I GRGCYC 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 608
FaeI CATG 4 cut(s) 113, 257, 456, 542
FalI AAGNNNNNCTT 2 cut(s) 217, 249
FaqI GGGAC 1 cut(s) 175
FatI CATG 4 cut(s) 109, 253, 452, 538
FblI GTMKAC 1 cut(s) 307
FokI GGATG 1 cut(s) 593
FriOI GRGCYC 1 cut(s) 85
GsuI CTGGAG 1 cut(s) 411
HaeIII GGCC 1 cut(s) 565
Hin1II CATG 4 cut(s) 113, 257, 456, 542
HincII GTYRAC 2 cut(s) 308, 370
HindII GTYRAC 2 cut(s) 308, 370
HphI GGTGA 1 cut(s) 189
Hpy166II GTNNAC 4 cut(s) 295, 308, 370, 517
Hpy188I TCNGA 2 cut(s) 205, 407
Hpy188III TCNNGA 5 cut(s) 110, 118, 493, 539, 572
Hpy8I GTNNAC 4 cut(s) 295, 308, 370, 517
HpyAV CCTTC 1 cut(s) 328
HpyCH4III ACNGT 2 cut(s) 445, 499
HpyCH4V TGCA 6 cut(s) 4, 37, 140, 456, 517, 560
HpyF3I CTNAG 1 cut(s) 459
Hsp92II CATG 4 cut(s) 113, 257, 456, 542
Kzo9I GATC 3 cut(s) 16, 221, 313
LmnI GCTCC 2 cut(s) 88, 508
LweI GCATC 1 cut(s) 127
MalI GATC 3 cut(s) 18, 223, 315
MboI GATC 3 cut(s) 16, 221, 313
MboII GAAGA 4 cut(s) 11, 109, 374, 597
MfeI CAATTG 1 cut(s) 12
MhlI GDGCHC 2 cut(s) 85, 519
MluCI AATT 4 cut(s) 12, 50, 288, 468
MmeI TCCRAC 1 cut(s) 334
MnlI CCTC 9 cut(s) 98, 117, 211, 358, 454, 459, 523, 544, 614
MseI TTAA 4 cut(s) 216, 384, 399, 631
MslI CAYNNNNRTG 1 cut(s) 119
MspR9I CCNGG 1 cut(s) 390
MunI CAATTG 1 cut(s) 12
Mva1269I GAATGC 2 cut(s) 193, 562
MvaI CCWGG 1 cut(s) 390
NdeII GATC 3 cut(s) 16, 221, 313
NlaIII CATG 4 cut(s) 113, 257, 456, 542
PagI TCATGA 2 cut(s) 109, 538
PctI GAATGC 2 cut(s) 193, 562
PfoI TCCNGGA 1 cut(s) 388
PshAI GACNNNNGTC 2 cut(s) 479, 592
PshBI ATTAAT 1 cut(s) 384
Psp124BI GAGCTC 1 cut(s) 85
Psp6I CCWGG 1 cut(s) 388
PspGI CCWGG 1 cut(s) 388
RsaI GTAC 2 cut(s) 296, 419
RsaNI GTAC 2 cut(s) 295, 418
RseI CAYNNNNRTG 1 cut(s) 119
SacI GAGCTC 1 cut(s) 85
SalI GTCGAC 1 cut(s) 306
SaqAI TTAA 4 cut(s) 216, 384, 399, 631
Sau3AI GATC 3 cut(s) 16, 221, 313
ScaI AGTACT 1 cut(s) 419
ScrFI CCNGG 1 cut(s) 390
SduI GDGCHC 2 cut(s) 85, 519
SfaNI GCATC 1 cut(s) 127
SfcI CTRYAG 2 cut(s) 169, 421
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 570
SmoI CTYRAG 1 cut(s) 570
Sse9I AATT 4 cut(s) 12, 50, 288, 468
SsiI CCGC 1 cut(s) 533
SspI AATATT 1 cut(s) 616
SstI GAGCTC 1 cut(s) 85
StyD4I CCNGG 1 cut(s) 388
StyI CCWWGG 1 cut(s) 608
TaaI ACNGT 2 cut(s) 445, 499
TaqI TCGA 2 cut(s) 263, 307
TasI AATT 4 cut(s) 12, 50, 288, 468
TatI WGTACW 2 cut(s) 294, 417
Tru1I TTAA 4 cut(s) 216, 384, 399, 631
Tru9I TTAA 4 cut(s) 216, 384, 399, 631
TscAI CASTG 2 cut(s) 463, 597
TspDTI ATGAA 3 cut(s) 233, 242, 527
TspGWI ACGGA 1 cut(s) 234
TspRI CASTG 2 cut(s) 463, 597
VneI GTGCAC 1 cut(s) 515
VspI ATTAAT 1 cut(s) 384
XmiI GTMKAC 1 cut(s) 307
ZrmI AGTACT 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.