pycom14g07090

Dna-directed rna polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0005882
Physical Location & Seq
Forward (+)
7074378 .. 7075022
645 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 597 bp
ATGGGTTCGAATCTAAGAGAAATTCTAGAGAATGTTTGCTACCTTGAAATATTCTTGTCTTTCCTGAATGATAAGGAGAAAAAAAAAATTGGCTCAAAAGAAAATGCCATTTTGGAGTTTTATCAACAATTTGCTTGTGTAGGCGGAGATCCGGTAGTTTCTAAATCCTTATGTAAGGAATTACAAAAGAAATTCTTTCAACAAAGATGTGAATTAGGAAGGAACAATACATTTTTGTTACCGCGAGATATATTAGCAGCTGCGGATCATTTGATTGGAATGAAATTTAGAATGGGTACCCTTGACGATATGAATCATTTGAAAAATAAACGTATTCGTTCTGTAGCGGATCTCTTACAAGATCAATTCAGATTGGCTCTGGTTCGTTTAGAAAATATGGTTAGAGGGACTATATGCGGAGCAATTAAGCATAAATTGATACCGACTCCTCAGAATTTGGTAACTTCAACTCCATTAACAACCACTTATGAATCTTTTTTCGGATTACACCCATTATCTCAAGTTTTGGATCGAACTAATCCATTGACACAAATAGTTCATGGAAGAAAATCGAGTTATCTGGCCCTGGAGGATTGA

Protein Analysis

199

Amino Acids

22.59

Weight (kDa)

9.11

Isoelectric Point (pI)

29.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb2_2 PF04561 1 - 113 2.4e-21 RNA polymerase Rpb2, domain 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 296
AccII CGCG 1 cut(s) 244
AciI CCGC 5 cut(s) 144, 242, 263, 347, 417
AclWI GGATC 4 cut(s) 143, 273, 357, 537
AcsI RAATTY 4 cut(s) 21, 191, 284, 454
AfaI GTAC 1 cut(s) 298
AgsI TTSAA 4 cut(s) 47, 200, 322, 468
AjnI CCWGG 1 cut(s) 585
AluBI AGCT 1 cut(s) 260
AluI AGCT 1 cut(s) 260
AlwI GGATC 4 cut(s) 143, 273, 357, 537
AoxI GGCC 1 cut(s) 582
ApeKI GCWGC 2 cut(s) 257, 260
ApoI RAATTY 4 cut(s) 21, 191, 284, 454
Asp718I GGTACC 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 583
AsuII TTCGAA 1 cut(s) 8
BanI GGYRCC 1 cut(s) 296
BarI GAAGNNNNNNTAC 2 cut(s) 211, 243
BbvI GCAGC 2 cut(s) 247, 269
BciT130I CCWGG 1 cut(s) 587
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 1 cut(s) 342
BisI GCNGC 2 cut(s) 258, 261
BlsI GCNGC 2 cut(s) 259, 262
Bme1390I CCNGG 1 cut(s) 587
BmgT120I GGNCC 1 cut(s) 583
BmiI GGNNCC 1 cut(s) 298
BmrFI CCNGG 1 cut(s) 587
Bpu14I TTCGAA 1 cut(s) 8
BpuEI CTTGAG 1 cut(s) 504
BsaBI GATNNNNATC 1 cut(s) 312
BsaJI CCNNGG 1 cut(s) 585
BsaWI WCCGGW 1 cut(s) 151
BsaXI ACNNNNNCTCC 4 cut(s) 138, 168, 454, 484
Bse8I GATNNNNATC 1 cut(s) 312
BseBI CCWGG 1 cut(s) 587
BseDI CCNNGG 1 cut(s) 585
BseJI GATNNNNATC 1 cut(s) 312
BseMII CTCAG 1 cut(s) 464
BseRI GAGGAG 1 cut(s) 438
BseXI GCAGC 2 cut(s) 247, 269
Bsh1236I CGCG 1 cut(s) 244
BshFI GGCC 1 cut(s) 584
BshNI GGYRCC 1 cut(s) 296
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 1 cut(s) 421
BsmFI GGGAC 1 cut(s) 421
BsnI GGCC 1 cut(s) 584
Bsp119I TTCGAA 1 cut(s) 8
Bsp143I GATC 5 cut(s) 148, 265, 349, 361, 529
BspACI CCGC 5 cut(s) 144, 242, 263, 347, 417
BspANI GGCC 1 cut(s) 584
BspCNI CTCAG 1 cut(s) 463
BspFNI CGCG 1 cut(s) 244
BspLI GGNNCC 1 cut(s) 298
BspPI GGATC 4 cut(s) 143, 273, 357, 537
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 1 cut(s) 296
BssECI CCNNGG 1 cut(s) 585
BssMI GATC 5 cut(s) 148, 265, 349, 361, 529
Bst2UI CCWGG 1 cut(s) 587
BstBI TTCGAA 1 cut(s) 8
BstDEI CTNAG 2 cut(s) 14, 450
BstFNI CGCG 1 cut(s) 244
BstKTI GATC 5 cut(s) 151, 268, 352, 364, 532
BstMBI GATC 5 cut(s) 148, 265, 349, 361, 529
BstNI CCWGG 1 cut(s) 587
BstSCI CCNGG 1 cut(s) 585
BstSFI CTRYAG 1 cut(s) 342
BstUI CGCG 1 cut(s) 244
BstV1I GCAGC 2 cut(s) 247, 269
BstX2I RGATCY 2 cut(s) 148, 349
BstYI RGATCY 2 cut(s) 148, 349
BsuRI GGCC 1 cut(s) 584
Cfr13I GGNCC 1 cut(s) 583
Csp6I GTAC 1 cut(s) 297
CviAII CATG 1 cut(s) 560
CviJI RGCY 4 cut(s) 93, 260, 377, 584
CviKI_1 RGCY 4 cut(s) 93, 260, 377, 584
CviQI GTAC 1 cut(s) 297
DdeI CTNAG 2 cut(s) 14, 450
DpnI GATC 5 cut(s) 150, 267, 351, 363, 531
DpnII GATC 5 cut(s) 148, 265, 349, 361, 529
EciI GGCGGA 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 585
FaeI CATG 1 cut(s) 563
FaiI YATR 9 cut(s) 172, 251, 311, 398, 413, 415, 432, 489, 561
FalI AAGNNNNNCTT 2 cut(s) 179, 211
FaqI GGGAC 1 cut(s) 421
FatI CATG 1 cut(s) 559
Fnu4HI GCNGC 2 cut(s) 258, 261
Fsp4HI GCNGC 2 cut(s) 258, 261
FspBI CTAG 1 cut(s) 26
GluI GCNGC 2 cut(s) 258, 261
HaeIII GGCC 1 cut(s) 584
HapII CCGG 1 cut(s) 152
Hin1II CATG 1 cut(s) 563
HinfI GANTC 4 cut(s) 10, 313, 445, 491
HpaII CCGG 1 cut(s) 152
Hpy188I TCNGA 3 cut(s) 371, 453, 503
Hpy188III TCNNGA 2 cut(s) 26, 64
HpyAV CCTTC 1 cut(s) 213
HpyCH4IV ACGT 1 cut(s) 331
HpyF3I CTNAG 2 cut(s) 14, 450
HpySE526I ACGT 1 cut(s) 331
Hsp92II CATG 1 cut(s) 563
KpnI GGTACC 1 cut(s) 300
Kzo9I GATC 5 cut(s) 148, 265, 349, 361, 529
LmnI GCTCC 1 cut(s) 419
LpnPI CCDG 5 cut(s) 77, 165, 365, 566, 572
Lsp1109I GCAGC 2 cut(s) 247, 269
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 331
MaeIII GTNAC 2 cut(s) 237, 460
MalI GATC 5 cut(s) 150, 267, 351, 363, 531
MboI GATC 5 cut(s) 148, 265, 349, 361, 529
MboII GAAGA 1 cut(s) 576
MflI RGATCY 2 cut(s) 148, 349
MlyI GAGTC 1 cut(s) 439
MnlI CCTC 3 cut(s) 398, 459, 583
MseI TTAA 2 cut(s) 426, 476
MspA1I CMGCKG 1 cut(s) 260
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 1 cut(s) 587
MvaI CCWGG 1 cut(s) 587
MvnI CGCG 1 cut(s) 244
NdeII GATC 5 cut(s) 148, 265, 349, 361, 529
NlaIII CATG 1 cut(s) 563
NlaIV GGNNCC 1 cut(s) 298
NspV TTCGAA 1 cut(s) 8
PfeI GAWTC 3 cut(s) 10, 313, 491
PkrI GCNGC 2 cut(s) 259, 262
PleI GAGTC 1 cut(s) 439
PpsI GAGTC 1 cut(s) 439
Psp6I CCWGG 1 cut(s) 585
PspGI CCWGG 1 cut(s) 585
PspN4I GGNNCC 1 cut(s) 298
PspPI GGNCC 1 cut(s) 583
PsuI RGATCY 2 cut(s) 148, 349
PvuII CAGCTG 1 cut(s) 260
RsaI GTAC 1 cut(s) 298
RsaNI GTAC 1 cut(s) 297
SaqAI TTAA 2 cut(s) 426, 476
SatI GCNGC 2 cut(s) 258, 261
Sau3AI GATC 5 cut(s) 148, 265, 349, 361, 529
Sau96I GGNCC 1 cut(s) 583
SchI GAGTC 1 cut(s) 439
ScrFI CCNGG 1 cut(s) 587
SetI ASST 3 cut(s) 45, 262, 334
SfcI CTRYAG 1 cut(s) 342
SfuI TTCGAA 1 cut(s) 8
SmlI CTYRAG 1 cut(s) 519
SmoI CTYRAG 1 cut(s) 519
SsiI CCGC 5 cut(s) 144, 242, 263, 347, 417
SspI AATATT 1 cut(s) 51
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 585
TaiI ACGT 1 cut(s) 334
TaqI TCGA 3 cut(s) 8, 532, 572
TfiI GAWTC 3 cut(s) 10, 313, 491
Tru1I TTAA 2 cut(s) 426, 476
Tru9I TTAA 2 cut(s) 426, 476
TseI GCWGC 2 cut(s) 257, 260
TspDTI ATGAA 4 cut(s) 296, 326, 504, 548
XapI RAATTY 4 cut(s) 21, 191, 284, 454
XbaI TCTAGA 1 cut(s) 25
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.