Rh2AG293900

Dna-directed rna polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0005882
Physical Location & Seq
Forward (+)
36264360 .. 36264761
402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG293900.1

Sequence Viewer

Length: 402 bp
ATGAACCGAAGACTGAATCTTGATATCCCTCAGAACAACACATTTTTGTTACCGCGAGATATATTGGCAGCAGCAGATCATTTGATTGGAAGGATATTTGGAATGGGTACACTTGACGATATGAATCATTTGAAAAATAAATGTATTCGGTCTATCACGGATCTTTTACAAGACCAATTCGGATTGGCCCTGGTTCATTTAGAAAATATGGTTAGAGGGACTATATGCGGAGCAATTAAGCATAAATTGATACCCACTCCTCATAATTTGGTAACTTCAACTCCATTAACAACCACTTATGAATCTTTTTTTGGATTGCACCCATTATCTCAAGTTTTAGATCAAACTAATCCATTGACCCAAATAGTTCATGGGAGAAAATTGAATTATCTGGGCCCTTGA

Protein Analysis

133

Amino Acids

14.92

Weight (kDa)

8.71

Isoelectric Point (pI)

21.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb2_2 PF04561 1 - 50 3.4e-10 RNA polymerase Rpb2, domain 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 55
AciI CCGC 2 cut(s) 53, 228
AclWI GGATC 1 cut(s) 168
AfaI GTAC 1 cut(s) 109
AgsI TTSAA 3 cut(s) 133, 279, 385
AjnI CCWGG 1 cut(s) 189
AjuI GAANNNNNNNTTGG 2 cut(s) 294, 326
AlwI GGATC 1 cut(s) 168
AoxI GGCC 2 cut(s) 186, 394
ApaI GGGCCC 1 cut(s) 398
ApeKI GCWGC 2 cut(s) 68, 71
AspS9I GGNCC 3 cut(s) 187, 394, 395
BaeGI GKGCMC 1 cut(s) 398
BanII GRGCYC 1 cut(s) 398
BbsI GAAGAC 1 cut(s) 16
BbvI GCAGC 2 cut(s) 80, 83
BciT130I CCWGG 1 cut(s) 191
BisI GCNGC 2 cut(s) 69, 72
BlsI GCNGC 2 cut(s) 70, 73
Bme1390I CCNGG 1 cut(s) 191
BmgT120I GGNCC 3 cut(s) 187, 394, 395
BmiI GGNNCC 1 cut(s) 396
BmrFI CCNGG 1 cut(s) 191
BpiI GAAGAC 1 cut(s) 16
BpuEI CTTGAG 1 cut(s) 315
BsaBI GATNNNNATC 1 cut(s) 123
BsaJI CCNNGG 1 cut(s) 189
BsaXI ACNNNNNCTCC 2 cut(s) 265, 295
Bse8I GATNNNNATC 1 cut(s) 123
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 1 cut(s) 189
BseJI GATNNNNATC 1 cut(s) 123
BseMII CTCAG 1 cut(s) 44
BseRI GAGGAG 1 cut(s) 249
BseSI GKGCMC 1 cut(s) 398
BseXI GCAGC 2 cut(s) 80, 83
Bsh1236I CGCG 1 cut(s) 55
BshFI GGCC 2 cut(s) 188, 396
BslFI GGGAC 1 cut(s) 232
BsmFI GGGAC 1 cut(s) 232
BsnI GGCC 2 cut(s) 188, 396
Bsp120I GGGCCC 1 cut(s) 394
Bsp1286I GDGCHC 1 cut(s) 398
Bsp143I GATC 3 cut(s) 76, 160, 340
BspACI CCGC 2 cut(s) 53, 228
BspANI GGCC 2 cut(s) 188, 396
BspCNI CTCAG 1 cut(s) 43
BspFNI CGCG 1 cut(s) 55
BspLI GGNNCC 1 cut(s) 396
BspPI GGATC 1 cut(s) 168
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 3 cut(s) 76, 160, 340
Bst2UI CCWGG 1 cut(s) 191
BstDEI CTNAG 1 cut(s) 30
BstFNI CGCG 1 cut(s) 55
BstKTI GATC 3 cut(s) 79, 163, 343
BstMBI GATC 3 cut(s) 76, 160, 340
BstNI CCWGG 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 189
BstSLI GKGCMC 1 cut(s) 398
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 2 cut(s) 80, 83
BstV2I GAAGAC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 160
BstYI RGATCY 1 cut(s) 160
BsuRI GGCC 2 cut(s) 188, 396
Cfr13I GGNCC 3 cut(s) 187, 394, 395
Csp6I GTAC 1 cut(s) 108
CviAII CATG 1 cut(s) 371
CviJI RGCY 2 cut(s) 188, 396
CviKI_1 RGCY 2 cut(s) 188, 396
CviQI GTAC 1 cut(s) 108
DdeI CTNAG 1 cut(s) 30
DpnI GATC 3 cut(s) 78, 162, 342
DpnII GATC 3 cut(s) 76, 160, 340
Eco24I GRGCYC 1 cut(s) 398
Eco32I GATATC 1 cut(s) 25
EcoO109I RGGNCCY 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 189
EcoRV GATATC 1 cut(s) 25
EcoT38I GRGCYC 1 cut(s) 398
FaeI CATG 1 cut(s) 374
FaiI YATR 9 cut(s) 62, 122, 209, 224, 226, 243, 264, 300, 372
FaqI GGGAC 1 cut(s) 232
FatI CATG 1 cut(s) 370
Fnu4HI GCNGC 2 cut(s) 69, 72
FriOI GRGCYC 1 cut(s) 398
Fsp4HI GCNGC 2 cut(s) 69, 72
GluI GCNGC 2 cut(s) 69, 72
HaeIII GGCC 2 cut(s) 188, 396
Hin1II CATG 1 cut(s) 374
HinfI GANTC 3 cut(s) 16, 124, 302
Hpy166II GTNNAC 1 cut(s) 110
Hpy188I TCNGA 2 cut(s) 33, 182
Hpy188III TCNNGA 1 cut(s) 20
Hpy8I GTNNAC 1 cut(s) 110
HpyAV CCTTC 1 cut(s) 84
HpyCH4V TGCA 1 cut(s) 319
HpyF3I CTNAG 1 cut(s) 30
Hsp92II CATG 1 cut(s) 374
Kzo9I GATC 3 cut(s) 76, 160, 340
LmnI GCTCC 1 cut(s) 230
LpnPI CCDG 3 cut(s) 176, 203, 377
Lsp1109I GCAGC 2 cut(s) 80, 83
MaeIII GTNAC 2 cut(s) 48, 271
MalI GATC 3 cut(s) 78, 162, 342
MboI GATC 3 cut(s) 76, 160, 340
MboII GAAGA 1 cut(s) 21
MflI RGATCY 1 cut(s) 160
MhlI GDGCHC 1 cut(s) 398
MluCI AATT 6 cut(s) 176, 234, 245, 265, 380, 385
MnlI CCTC 3 cut(s) 39, 209, 270
MseI TTAA 2 cut(s) 237, 287
MspR9I CCNGG 1 cut(s) 191
MvaI CCWGG 1 cut(s) 191
MvnI CGCG 1 cut(s) 55
NdeII GATC 3 cut(s) 76, 160, 340
NlaIII CATG 1 cut(s) 374
NlaIV GGNNCC 1 cut(s) 396
PfeI GAWTC 3 cut(s) 16, 124, 302
PkrI GCNGC 2 cut(s) 70, 73
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 1 cut(s) 396
PspOMI GGGCCC 1 cut(s) 394
PspPI GGNCC 3 cut(s) 187, 394, 395
PsuI RGATCY 1 cut(s) 160
RsaI GTAC 1 cut(s) 109
RsaNI GTAC 1 cut(s) 108
SaqAI TTAA 2 cut(s) 237, 287
SatI GCNGC 2 cut(s) 69, 72
Sau3AI GATC 3 cut(s) 76, 160, 340
Sau96I GGNCC 3 cut(s) 187, 394, 395
ScrFI CCNGG 1 cut(s) 191
SduI GDGCHC 1 cut(s) 398
SmlI CTYRAG 1 cut(s) 330
SmoI CTYRAG 1 cut(s) 330
Sse9I AATT 6 cut(s) 176, 234, 245, 265, 380, 385
SsiI CCGC 2 cut(s) 53, 228
StyD4I CCNGG 1 cut(s) 189
TaqII GACCGA 1 cut(s) 138
TasI AATT 6 cut(s) 176, 234, 245, 265, 380, 385
TfiI GAWTC 3 cut(s) 16, 124, 302
Tru1I TTAA 2 cut(s) 237, 287
Tru9I TTAA 2 cut(s) 237, 287
TseI GCWGC 2 cut(s) 68, 71
TspDTI ATGAA 5 cut(s) 17, 137, 185, 315, 359
TspGWI ACGGA 1 cut(s) 173
XcmI CCANNNNNNNNNTGG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.