pycom14g07470

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004800
Physical Location & Seq
Forward (+)
7639705 .. 7641065
1361 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g07470.2

Sequence Viewer

Length: 786 bp
ATGTCGGAGCTGTTCATAAAACAATCGAAGCAATATGCCGAGGGTCGGCCATGCTACCCGAAAGAGCTGTTTGAATACATTGCCTCCAAGACCCCTTGTCACGACCTCGCATGGGACGTCGGCACCGGCAATGGCCAGGCTGCCCGATCCCTGCCCAAGATCTTCAAGAATGTGATAGCCACTGATACAAGCCAAAAACAACTCGAGTTCGCACCCAAGCTGCCCAATATTCGCTACGAGCACACCCCTCCGGTCATGTCAATAGCCGAGGTTGAACAAAAGTTGACACCAAAATCAAGTGTTGATCTGGTGACCGTTGCTCAGGCCATCCATTGGTTTGACCTCCCCAATTTCTACCAACAAGTGAAACATGTACTGAAGAAACCTAATGGTGTCCTTGCAGCATGGTGCTATACAGTGCCTAGGGTTAACAGCATTGTAGATACCGTCTTTGATCGTCTTTACACCGTTGATTCTGATCCTTACTGGGAGCCACCACGTAAAATGGTGGACGACAAGTATGAGAGTATCGATTTTCCATTTGAGCCGGTGGACGGAGAAGAGAACACAGGACCGTTTGAGTTCGTGACAGAGCAGTTGATGGATTTGGATGGCTTTTTTACATACATAAGGTCATGGTCTGGATATCACACGGCAAAAGGAAGGGGTGTTGAGCTCTTGAGTGATCATGTGATTGAGGCATTTAAGCAAGCGTGGAATGAAGGTGGAAACCATGGTCAGAAGGCTGTCAAGTTTCCTGTCTATTTGAGAATTGGAAAAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

262

Amino Acids

29.83

Weight (kDa)

5.83

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 120
AccB1I GGYRCC 1 cut(s) 122
AccB7I CCANNNNNTGG 1 cut(s) 333
AclWI GGATC 2 cut(s) 141, 473
AcoI YGGCCR 2 cut(s) 47, 133
AcuI CTGAAG 1 cut(s) 398
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 1 cut(s) 375
AfiI CCNNNNNNNGG 4 cut(s) 45, 112, 333, 554
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 3 cut(s) 74, 166, 275
AhdI GACNNNNNGTC 1 cut(s) 96
AjnI CCWGG 1 cut(s) 135
AluBI AGCT 4 cut(s) 10, 67, 220, 676
AluI AGCT 4 cut(s) 10, 67, 220, 676
Alw21I GWGCWC 2 cut(s) 243, 678
AlwI GGATC 2 cut(s) 141, 473
Ama87I CYCGRG 1 cut(s) 203
AoxI GGCC 3 cut(s) 47, 133, 324
ApeKI GCWGC 3 cut(s) 140, 220, 401
AspA2I CCTAGG 1 cut(s) 422
AspS9I GGNCC 1 cut(s) 572
AsuHPI GGTGA 1 cut(s) 322
AvaI CYCGRG 1 cut(s) 203
AvaII GGWCC 1 cut(s) 572
AvrII CCTAGG 1 cut(s) 422
BalI TGGCCA 1 cut(s) 135
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 678
Bbv12I GWGCWC 2 cut(s) 243, 678
BbvI GCAGC 3 cut(s) 127, 207, 413
BccI CCATC 3 cut(s) 335, 595, 605
BceAI ACGGC 1 cut(s) 669
BciT130I CCWGG 1 cut(s) 137
BclI TGATCA 1 cut(s) 685
BfaI CTAG 1 cut(s) 423
BglII AGATCT 1 cut(s) 159
BisI GCNGC 3 cut(s) 141, 221, 402
BlnI CCTAGG 1 cut(s) 422
BlsI GCNGC 3 cut(s) 142, 222, 403
Bme1390I CCNGG 1 cut(s) 137
Bme18I GGWCC 1 cut(s) 572
BmeRI GACNNNNNGTC 1 cut(s) 96
BmeT110I CYCGRG 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 572
BmiI GGNNCC 2 cut(s) 124, 492
BmrFI CCNGG 1 cut(s) 137
BmrI ACTGGG 1 cut(s) 496
BmuI ACTGGG 1 cut(s) 496
Bpu10I CCTNAGC 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 700
Bsa29I ATCGAT 1 cut(s) 531
BsaAI YACGTR 1 cut(s) 500
BsaBI GATNNNNATC 1 cut(s) 477
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 4 cut(s) 39, 267, 422, 733
BsaWI WCCGGW 1 cut(s) 250
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 4 cut(s) 45, 112, 333, 554
Bse118I RCCGGY 2 cut(s) 125, 547
Bse1I ACTGG 1 cut(s) 491
Bse3DI GCAATG 2 cut(s) 78, 136
Bse8I GATNNNNATC 1 cut(s) 477
BseBI CCWGG 1 cut(s) 137
BseCI ATCGAT 1 cut(s) 531
BseDI CCNNGG 4 cut(s) 39, 267, 422, 733
BseGI GGATG 2 cut(s) 327, 616
BseJI GATNNNNATC 1 cut(s) 477
BseLI CCNNNNNNNGG 4 cut(s) 45, 112, 333, 554
BseMI GCAATG 2 cut(s) 78, 136
BseMII CTCAG 1 cut(s) 335
BseNI ACTGG 1 cut(s) 491
BseXI GCAGC 3 cut(s) 127, 207, 413
BshFI GGCC 3 cut(s) 49, 135, 326
BshNI GGYRCC 1 cut(s) 122
BshVI ATCGAT 1 cut(s) 531
BsiHKAI GWGCWC 2 cut(s) 243, 678
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 3 cut(s) 126, 251, 548
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 4 cut(s) 45, 112, 333, 554
BsmFI GGGAC 1 cut(s) 128
BsnI GGCC 3 cut(s) 49, 135, 326
BsoBI CYCGRG 1 cut(s) 203
Bsp1286I GDGCHC 2 cut(s) 243, 678
Bsp143I GATC 6 cut(s) 146, 159, 304, 454, 478, 685
Bsp19I CCATGG 1 cut(s) 733
BspANI GGCC 3 cut(s) 49, 135, 326
BspCNI CTCAG 1 cut(s) 334
BspDI ATCGAT 1 cut(s) 531
BspLI GGNNCC 2 cut(s) 124, 492
BspPI GGATC 2 cut(s) 141, 473
BspT107I GGYRCC 1 cut(s) 122
BsrDI GCAATG 2 cut(s) 78, 136
BsrFI RCCGGY 2 cut(s) 125, 547
BsrI ACTGG 1 cut(s) 491
BssAI RCCGGY 2 cut(s) 125, 547
BssECI CCNNGG 4 cut(s) 39, 267, 422, 733
BssMI GATC 6 cut(s) 146, 159, 304, 454, 478, 685
BssNI GRCGYC 1 cut(s) 117
BssT1I CCWWGG 2 cut(s) 422, 733
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 5 cut(s) 316, 418, 448, 469, 576
Bst6I CTCTTC 1 cut(s) 555
BstACI GRCGYC 1 cut(s) 117
BstBAI YACGTR 1 cut(s) 500
BstC8I GCNNGC 1 cut(s) 711
BstDEI CTNAG 1 cut(s) 321
BstDSI CCRYGG 1 cut(s) 733
BstEII GGTNACC 1 cut(s) 310
BstF5I GGATG 2 cut(s) 327, 616
BstKTI GATC 6 cut(s) 149, 162, 307, 457, 481, 688
BstMBI GATC 6 cut(s) 146, 159, 304, 454, 478, 685
BstNI CCWGG 1 cut(s) 137
BstNSI RCATGY 1 cut(s) 374
BstPI GGTNACC 1 cut(s) 310
BstSCI CCNGG 1 cut(s) 135
BstV1I GCAGC 3 cut(s) 127, 207, 413
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
Bsu15I ATCGAT 1 cut(s) 531
BsuRI GGCC 3 cut(s) 49, 135, 326
BsuTUI ATCGAT 1 cut(s) 531
BtgI CCRYGG 1 cut(s) 733
BtsCI GGATG 2 cut(s) 327, 616
BtsIMutI CAGTG 2 cut(s) 180, 423
Cac8I GCNNGC 1 cut(s) 711
Cfr10I RCCGGY 2 cut(s) 125, 547
Cfr13I GGNCC 1 cut(s) 572
ClaI ATCGAT 1 cut(s) 531
Csp6I GTAC 1 cut(s) 374
CviAII CATG 8 cut(s) 51, 111, 256, 371, 405, 636, 689, 734
CviQI GTAC 1 cut(s) 374
DdeI CTNAG 1 cut(s) 321
DpnI GATC 6 cut(s) 148, 161, 306, 456, 480, 687
DpnII GATC 6 cut(s) 146, 159, 304, 454, 478, 685
DriI GACNNNNNGTC 1 cut(s) 96
EaeI YGGCCR 2 cut(s) 47, 133
Eam1104I CTCTTC 1 cut(s) 555
Eam1105I GACNNNNNGTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 555
Ecl136II GAGCTC 1 cut(s) 676
Eco130I CCWWGG 2 cut(s) 422, 733
Eco24I GRGCYC 1 cut(s) 678
Eco32I GATATC 1 cut(s) 647
Eco47I GGWCC 1 cut(s) 572
Eco53kI GAGCTC 1 cut(s) 676
Eco57I CTGAAG 1 cut(s) 398
Eco88I CYCGRG 1 cut(s) 203
Eco91I GGTNACC 1 cut(s) 310
EcoICRI GAGCTC 1 cut(s) 676
EcoO65I GGTNACC 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 135
EcoRV GATATC 1 cut(s) 647
EcoT14I CCWWGG 2 cut(s) 422, 733
EcoT38I GRGCYC 1 cut(s) 678
ErhI CCWWGG 2 cut(s) 422, 733
FaeI CATG 8 cut(s) 54, 114, 259, 374, 408, 639, 692, 737
FaqI GGGAC 1 cut(s) 128
FatI CATG 8 cut(s) 50, 110, 255, 370, 404, 635, 688, 733
FbaI TGATCA 1 cut(s) 685
Fnu4HI GCNGC 3 cut(s) 141, 221, 402
FokI GGATG 2 cut(s) 314, 623
FriOI GRGCYC 1 cut(s) 678
Fsp4HI GCNGC 3 cut(s) 141, 221, 402
FspBI CTAG 1 cut(s) 423
GluI GCNGC 3 cut(s) 141, 221, 402
HaeIII GGCC 3 cut(s) 49, 135, 326
HapII CCGG 3 cut(s) 126, 251, 548
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 8 cut(s) 54, 114, 259, 374, 408, 639, 692, 737
HincII GTYRAC 2 cut(s) 285, 430
HindII GTYRAC 2 cut(s) 285, 430
HinfI GANTC 1 cut(s) 473
HpaI GTTAAC 1 cut(s) 430
HpaII CCGG 3 cut(s) 126, 251, 548
HphI GGTGA 1 cut(s) 322
Hpy166II GTNNAC 4 cut(s) 285, 430, 511, 553
Hpy188I TCNGA 3 cut(s) 7, 478, 741
Hpy188III TCNNGA 5 cut(s) 101, 166, 586, 642, 679
Hpy8I GTNNAC 4 cut(s) 285, 430, 511, 553
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 3 cut(s) 657, 716, 736
HpyCH4III ACNGT 5 cut(s) 316, 418, 448, 469, 576
HpyCH4IV ACGT 2 cut(s) 117, 499
HpyCH4V TGCA 1 cut(s) 401
HpyF3I CTNAG 1 cut(s) 321
HpySE526I ACGT 2 cut(s) 117, 499
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 8 cut(s) 54, 114, 259, 374, 408, 639, 692, 737
Ksp22I TGATCA 1 cut(s) 685
KspAI GTTAAC 1 cut(s) 430
Kzo9I GATC 6 cut(s) 146, 159, 304, 454, 478, 685
LmnI GCTCC 2 cut(s) 7, 490
Lsp1109I GCAGC 3 cut(s) 127, 207, 413
MaeI CTAG 1 cut(s) 423
MaeII ACGT 2 cut(s) 117, 499
MaeIII GTNAC 3 cut(s) 98, 310, 586
MalI GATC 6 cut(s) 148, 161, 306, 456, 480, 687
MboI GATC 6 cut(s) 146, 159, 304, 454, 478, 685
MboII GAAGA 3 cut(s) 154, 391, 572
MflI RGATCY 1 cut(s) 159
MhlI GDGCHC 2 cut(s) 243, 678
MlsI TGGCCA 1 cut(s) 135
MluCI AATT 2 cut(s) 349, 771
MluNI TGGCCA 1 cut(s) 135
MnlI CCTC 7 cut(s) 34, 94, 116, 258, 262, 353, 691
Mox20I TGGCCA 1 cut(s) 135
MscI TGGCCA 1 cut(s) 135
MseI TTAA 2 cut(s) 429, 705
Msp20I TGGCCA 1 cut(s) 135
MspI CCGG 3 cut(s) 126, 251, 548
MspR9I CCNGG 1 cut(s) 137
MvaI CCWGG 1 cut(s) 137
NcoI CCATGG 1 cut(s) 733
NdeII GATC 6 cut(s) 146, 159, 304, 454, 478, 685
NlaIII CATG 8 cut(s) 54, 114, 259, 374, 408, 639, 692, 737
NlaIV GGNNCC 2 cut(s) 124, 492
NmeAIII GCCGAG 2 cut(s) 64, 292
NmuCI GTSAC 3 cut(s) 98, 310, 586
NspI RCATGY 1 cut(s) 374
PaeR7I CTCGAG 1 cut(s) 203
PciI ACATGT 1 cut(s) 370
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PfeI GAWTC 1 cut(s) 473
PflMI CCANNNNNTGG 1 cut(s) 333
PkrI GCNGC 3 cut(s) 142, 222, 403
Ppu21I YACGTR 1 cut(s) 500
PscI ACATGT 1 cut(s) 370
Psp124BI GAGCTC 1 cut(s) 678
Psp6I CCWGG 1 cut(s) 135
PspEI GGTNACC 1 cut(s) 310
PspGI CCWGG 1 cut(s) 135
PspN4I GGNNCC 2 cut(s) 124, 492
PspPI GGNCC 1 cut(s) 572
PspXI VCTCGAGB 1 cut(s) 203
PsuI RGATCY 1 cut(s) 159
RsaI GTAC 1 cut(s) 375
RsaNI GTAC 1 cut(s) 374
SacI GAGCTC 1 cut(s) 678
SaqAI TTAA 2 cut(s) 429, 705
SatI GCNGC 3 cut(s) 141, 221, 402
Sau3AI GATC 6 cut(s) 146, 159, 304, 454, 478, 685
Sau96I GGNCC 1 cut(s) 572
ScrFI CCNGG 1 cut(s) 137
SduI GDGCHC 2 cut(s) 243, 678
Sfr274I CTCGAG 1 cut(s) 203
SinI GGWCC 1 cut(s) 572
SlaI CTCGAG 1 cut(s) 203
SmlI CTYRAG 2 cut(s) 203, 679
SmoI CTYRAG 2 cut(s) 203, 679
Sse9I AATT 2 cut(s) 349, 771
SspI AATATT 1 cut(s) 229
SspMI CTAG 1 cut(s) 423
SstI GAGCTC 1 cut(s) 678
StyD4I CCNGG 1 cut(s) 135
StyI CCWWGG 2 cut(s) 422, 733
TaaI ACNGT 5 cut(s) 316, 418, 448, 469, 576
TaiI ACGT 2 cut(s) 120, 502
TaqI TCGA 3 cut(s) 26, 204, 531
TasI AATT 2 cut(s) 349, 771
TatI WGTACW 1 cut(s) 373
TfiI GAWTC 1 cut(s) 473
Tru1I TTAA 2 cut(s) 429, 705
Tru9I TTAA 2 cut(s) 429, 705
TscAI CASTG 2 cut(s) 187, 423
TseFI GTSAC 3 cut(s) 98, 310, 586
TseI GCWGC 3 cut(s) 140, 220, 401
Tsp45I GTSAC 3 cut(s) 98, 310, 586
TspDTI ATGAA 2 cut(s) 4, 735
TspGWI ACGGA 1 cut(s) 570
TspRI CASTG 2 cut(s) 187, 423
Van91I CCANNNNNTGG 1 cut(s) 333
VpaK11BI GGWCC 1 cut(s) 572
XceI RCATGY 1 cut(s) 374
XhoI CTCGAG 1 cut(s) 203
XmaJI CCTAGG 1 cut(s) 422
XspI CTAG 1 cut(s) 423
ZraI GACGTC 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.