pycom14g07840

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010201
Physical Location & Seq
Forward (+)
8137231 .. 8139561
2331 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1182 bp
ATGGCGACCAACTCCGGGTCACTGAATTATTGGAGGGATTACTTTCGGACTGCAAACTCTGATATTTTTGATATAATCGACCATGCGATCATGGTGGCGGCTTCCGATTGCCCCACGGAGTTGAGATTGCGGCGGGATCGGATAGCAGAGCGGTTGTTTTCCAGCAGATTGACCCGGTGGTGTTCGGGTTGCGAAAAACAAGTCGAATTGGCCACCGTGCCAGGCGGCGATGGAGAAGGACATGACGACGAAGGCGGCAGCGACGATGTGCACAGAGGGTGTAAAAGTGAGTTTGACGGAGGTGGAGGGGAAGTCGAGGGCGGTGGCAGTAAAGAGAGCAAGGTGAATAGCAGCAGCAGAGATGATGAGGGCGGGCTTAGCAGCGACAGCTATGGATTGGCTGAGGCTTTGACAGATGAGATTGAGCAAGAGTCTCAAACTCTTGGGGAGGTCTTCAGGATTAGAGACATGTTTCTTGATTACGAACAAGAGTCTGATACGGTATTGTTCGACTCTTTGAGGAAGCTTCAGTTGATGGCTCTGAATGTGGAGACTCTCGAGGCAACTGAAATTGGAAAGGCGGTTAGTGGTCTCAAAAAGCATGGATCAAAGCAGATTCGTCATCTTGCTCGAGAGCTTGTCTCAGGTTGGAAAGGCATGGTAGATGAATGGGTCAATGCTACAGCAGCTATTGCCAGCACGAAAACTATGTCAGATTCTGTTAATCCATCTTTTGATGATGAAGAAGGACTACCATCTCCTCCTCTTGATGAAGCTTTCCTTGCTAATCAAACCACCACAATAGAACTCTCTCAGTTCTTTGACGGCATGGATGATGATGGAAATCCTCGAAACAACGGGGAATTCACTGAGTACCGTGAAAATGGAAGAAAGCGAGAAGTGGAGAAAGAAAATACTATTAAGCAGAACCAACAATATTCAAATGAGGCAAATGTGCTTCCCAAGGGAAACAAGAATGAACAAGTGAAGAAACGAGAAGTTGTGAAGCCAAATAAGCCCTCAAACACTCACTATGGGCCTAGCAGACCTCCCAAAGTTAGTATGGATCAAAAGGTCAACAGTGAAACAAAGATCGAGCAAAAAACAGATAAGGTTGCCATTCAAAAGAGGCAACACGCTGGTCAGCAAAATGTAGGTGCTAAAATTCAAGGGTTCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med26

Protein Analysis

394

Amino Acids

43.65

Weight (kDa)

4.99

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med26 PF08711 172 - 221 2e-13 TFIIS helical bundle-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 151
AciI CCGC 9 cut(s) 98, 130, 133, 151, 225, 255, 321, 372, 581
AclWI GGATC 3 cut(s) 144, 613, 1074
AcoI YGGCCR 1 cut(s) 210
AcsI RAATTY 2 cut(s) 863, 1164
AcuI CTGAAG 2 cut(s) 439, 512
AfaI GTAC 1 cut(s) 875
AfiI CCNNNNNNNGG 1 cut(s) 15
AflIII ACRYGT 1 cut(s) 468
AgsI TTSAA 3 cut(s) 942, 1124, 1169
AjnI CCWGG 1 cut(s) 220
AluBI AGCT 5 cut(s) 390, 526, 637, 689, 776
AluI AGCT 5 cut(s) 390, 526, 637, 689, 776
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 5 cut(s) 438, 459, 545, 596, 646
Alw44I GTGCAC 1 cut(s) 269
AlwI GGATC 3 cut(s) 144, 613, 1074
AlwNI CAGNNNCTG 1 cut(s) 719
Ama87I CYCGRG 2 cut(s) 557, 630
AoxI GGCC 2 cut(s) 210, 1037
ApaLI GTGCAC 1 cut(s) 269
ApeKI GCWGC 5 cut(s) 258, 351, 354, 381, 686
ApoI RAATTY 2 cut(s) 863, 1164
ArsI GACNNNNNNTTYG 4 cut(s) 186, 218, 695, 727
AspS9I GGNCC 1 cut(s) 1037
AsuC2I CCSGG 2 cut(s) 16, 175
AsuHPI GGTGA 1 cut(s) 355
AvaI CYCGRG 2 cut(s) 557, 630
BaeGI GKGCMC 1 cut(s) 273
BalI TGGCCA 1 cut(s) 212
BarI GAAGNNNNNNTAC 2 cut(s) 735, 767
BbsI GAAGAC 1 cut(s) 445
Bbv12I GWGCWC 1 cut(s) 273
BbvCI CCTCAGC 1 cut(s) 402
BbvI GCAGC 5 cut(s) 270, 363, 366, 393, 698
BccI CCATC 5 cut(s) 224, 529, 736, 763, 833
BceAI ACGGC 1 cut(s) 841
BciT130I CCWGG 1 cut(s) 222
BcnI CCSGG 2 cut(s) 16, 175
BcoDI GTCTC 5 cut(s) 438, 459, 545, 596, 646
BfaI CTAG 1 cut(s) 1041
BfmI CTRYAG 1 cut(s) 681
BisI GCNGC 9 cut(s) 99, 131, 226, 256, 259, 352, 355, 382, 687
BlpI GCTNAGC 1 cut(s) 377
BlsI GCNGC 9 cut(s) 100, 132, 227, 257, 260, 353, 356, 383, 688
Bme1390I CCNGG 3 cut(s) 16, 175, 222
BmeT110I CYCGRG 2 cut(s) 557, 630
BmgT120I GGNCC 1 cut(s) 1037
BmrFI CCNGG 3 cut(s) 16, 175, 222
BpiI GAAGAC 1 cut(s) 445
BplI GAGNNNNNCTC 2 cut(s) 626, 658
Bpu10I CCTNAGC 1 cut(s) 402
Bpu1102I GCTNAGC 1 cut(s) 377
BpuMI CCSGG 2 cut(s) 16, 175
BsaBI GATNNNNATC 1 cut(s) 843
BsaI GGTCTC 1 cut(s) 596
BsaJI CCNNGG 2 cut(s) 114, 963
BsaXI ACNNNNNCTCC 2 cut(s) 297, 327
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse8I GATNNNNATC 1 cut(s) 843
BseBI CCWGG 1 cut(s) 222
BseDI CCNNGG 2 cut(s) 114, 963
BseGI GGATG 1 cut(s) 838
BseJI GATNNNNATC 1 cut(s) 843
BseLI CCNNNNNNNGG 1 cut(s) 15
BseMII CTCAG 4 cut(s) 393, 657, 827, 861
BseRI GAGGAG 2 cut(s) 750, 753
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 5 cut(s) 270, 363, 366, 393, 698
BshFI GGCC 2 cut(s) 212, 1039
BsiHKAI GWGCWC 1 cut(s) 273
BsiHKCI CYCGRG 2 cut(s) 557, 630
BsiSI CCGG 2 cut(s) 15, 175
BslI CCNNNNNNNGG 1 cut(s) 15
BsmAI GTCTC 5 cut(s) 438, 459, 545, 596, 646
BsnI GGCC 2 cut(s) 212, 1039
Bso31I GGTCTC 1 cut(s) 596
BsoBI CYCGRG 2 cut(s) 557, 630
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 5 cut(s) 87, 136, 605, 1066, 1092
Bsp1720I GCTNAGC 1 cut(s) 377
BspACI CCGC 9 cut(s) 98, 130, 133, 151, 225, 255, 321, 372, 581
BspANI GGCC 2 cut(s) 212, 1039
BspCNI CTCAG 4 cut(s) 394, 656, 826, 862
BspPI GGATC 3 cut(s) 144, 613, 1074
BspTNI GGTCTC 1 cut(s) 596
BsrBI CCGCTC 1 cut(s) 151
BssECI CCNNGG 2 cut(s) 114, 963
BssMI GATC 5 cut(s) 87, 136, 605, 1066, 1092
BssT1I CCWWGG 1 cut(s) 963
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 4 cut(s) 217, 502, 878, 1082
BstAPI GCANNNNNTGC 1 cut(s) 692
BstC8I GCNNGC 2 cut(s) 374, 697
BstDEI CTNAG 5 cut(s) 377, 402, 643, 813, 870
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 1 cut(s) 838
BstKTI GATC 5 cut(s) 90, 139, 608, 1069, 1095
BstMAI GTCTC 5 cut(s) 438, 459, 545, 596, 646
BstMBI GATC 5 cut(s) 87, 136, 605, 1066, 1092
BstMWI GCNNNNNNNGC 6 cut(s) 378, 387, 686, 692, 782, 1015
BstNI CCWGG 1 cut(s) 222
BstNSI RCATGY 1 cut(s) 472
BstSCI CCNGG 3 cut(s) 14, 173, 220
BstSFI CTRYAG 1 cut(s) 681
BstSLI GKGCMC 1 cut(s) 273
BstV1I GCAGC 5 cut(s) 270, 363, 366, 393, 698
BstV2I GAAGAC 1 cut(s) 445
BsuRI GGCC 2 cut(s) 212, 1039
BtgI CCRYGG 1 cut(s) 114
BtgZI GCGATG 1 cut(s) 243
BtsCI GGATG 1 cut(s) 838
BtsIMutI CAGTG 3 cut(s) 20, 867, 1087
Cac8I GCNNGC 2 cut(s) 374, 697
CaiI CAGNNNCTG 1 cut(s) 719
Cfr13I GGNCC 1 cut(s) 1037
Csp6I GTAC 1 cut(s) 874
CviAII CATG 7 cut(s) 83, 91, 242, 469, 602, 658, 829
CviQI GTAC 1 cut(s) 874
DdeI CTNAG 5 cut(s) 377, 402, 643, 813, 870
DpnI GATC 5 cut(s) 89, 138, 607, 1068, 1094
DpnII GATC 5 cut(s) 87, 136, 605, 1066, 1092
EaeI YGGCCR 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 963
Eco31I GGTCTC 1 cut(s) 596
Eco57I CTGAAG 2 cut(s) 439, 512
Eco88I CYCGRG 2 cut(s) 557, 630
EcoRI GAATTC 1 cut(s) 863
EcoRII CCWGG 1 cut(s) 220
EcoT14I CCWWGG 1 cut(s) 963
ErhI CCWWGG 1 cut(s) 963
FaeI CATG 7 cut(s) 86, 94, 245, 472, 605, 661, 832
FalI AAGNNNNNCTT 2 cut(s) 765, 797
FatI CATG 7 cut(s) 82, 90, 241, 468, 601, 657, 828
FauI CCCGC 2 cut(s) 126, 365
Fnu4HI GCNGC 9 cut(s) 99, 131, 226, 256, 259, 352, 355, 382, 687
FokI GGATG 1 cut(s) 845
Fsp4HI GCNGC 9 cut(s) 99, 131, 226, 256, 259, 352, 355, 382, 687
FspBI CTAG 1 cut(s) 1041
GluI GCNGC 9 cut(s) 99, 131, 226, 256, 259, 352, 355, 382, 687
HaeIII GGCC 2 cut(s) 212, 1039
HapII CCGG 2 cut(s) 15, 175
Hin1II CATG 7 cut(s) 86, 94, 245, 472, 605, 661, 832
HincII GTYRAC 1 cut(s) 1078
HindII GTYRAC 1 cut(s) 1078
HindIII AAGCTT 2 cut(s) 524, 774
HinfI GANTC 6 cut(s) 431, 491, 512, 553, 616, 716
HpaII CCGG 2 cut(s) 15, 175
HphI GGTGA 1 cut(s) 355
Hpy166II GTNNAC 2 cut(s) 271, 1078
Hpy188I TCNGA 8 cut(s) 48, 61, 106, 141, 496, 543, 715, 1178
Hpy188III TCNNGA 5 cut(s) 457, 476, 557, 632, 767
Hpy8I GTNNAC 2 cut(s) 271, 1078
Hpy99I CGWCG 2 cut(s) 251, 266
HpyAV CCTTC 3 cut(s) 230, 245, 740
HpyCH4III ACNGT 4 cut(s) 217, 502, 878, 1082
HpyCH4V TGCA 2 cut(s) 53, 271
HpyF10VI GCNNNNNNNGC 6 cut(s) 378, 387, 686, 692, 782, 1015
HpyF3I CTNAG 5 cut(s) 377, 402, 643, 813, 870
Hsp92II CATG 7 cut(s) 86, 94, 245, 472, 605, 661, 832
Kzo9I GATC 5 cut(s) 87, 136, 605, 1066, 1092
LpnPI CCDG 9 cut(s) 28, 175, 188, 207, 234, 442, 630, 709, 1125
Lsp1109I GCAGC 5 cut(s) 270, 363, 366, 393, 698
MaeI CTAG 1 cut(s) 1041
MaeIII GTNAC 1 cut(s) 18
MalI GATC 5 cut(s) 89, 138, 607, 1068, 1094
MbiI CCGCTC 1 cut(s) 151
MboI GATC 5 cut(s) 87, 136, 605, 1066, 1092
MboII GAAGA 4 cut(s) 445, 755, 900, 1000
MhlI GDGCHC 1 cut(s) 273
MlsI TGGCCA 1 cut(s) 212
MluCI AATT 5 cut(s) 25, 206, 570, 863, 1164
MluNI TGGCCA 1 cut(s) 212
MlyI GAGTC 4 cut(s) 440, 500, 506, 547
MmeI TCCRAC 1 cut(s) 629
Mox20I TGGCCA 1 cut(s) 212
MscI TGGCCA 1 cut(s) 212
MseI TTAA 2 cut(s) 723, 921
Msp20I TGGCCA 1 cut(s) 212
MspI CCGG 2 cut(s) 15, 175
MspR9I CCNGG 3 cut(s) 16, 175, 222
MvaI CCWGG 1 cut(s) 222
MwoI GCNNNNNNNGC 6 cut(s) 378, 387, 686, 692, 782, 1015
NciI CCSGG 2 cut(s) 16, 175
NdeII GATC 5 cut(s) 87, 136, 605, 1066, 1092
NlaIII CATG 7 cut(s) 86, 94, 245, 472, 605, 661, 832
NmuCI GTSAC 1 cut(s) 18
NspI RCATGY 1 cut(s) 472
PaeR7I CTCGAG 2 cut(s) 557, 630
PciI ACATGT 1 cut(s) 468
PfeI GAWTC 2 cut(s) 616, 716
PkrI GCNGC 9 cut(s) 100, 132, 227, 257, 260, 353, 356, 383, 688
PleI GAGTC 4 cut(s) 439, 499, 506, 547
PpsI GAGTC 4 cut(s) 439, 499, 506, 547
PscI ACATGT 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspPI GGNCC 1 cut(s) 1037
PstNI CAGNNNCTG 1 cut(s) 719
RsaI GTAC 1 cut(s) 875
RsaNI GTAC 1 cut(s) 874
SaqAI TTAA 2 cut(s) 723, 921
SatI GCNGC 9 cut(s) 99, 131, 226, 256, 259, 352, 355, 382, 687
Sau3AI GATC 5 cut(s) 87, 136, 605, 1066, 1092
Sau96I GGNCC 1 cut(s) 1037
SchI GAGTC 4 cut(s) 440, 500, 506, 547
ScrFI CCNGG 3 cut(s) 16, 175, 222
SduI GDGCHC 1 cut(s) 273
SfcI CTRYAG 1 cut(s) 681
Sfr274I CTCGAG 2 cut(s) 557, 630
SlaI CTCGAG 2 cut(s) 557, 630
SmlI CTYRAG 2 cut(s) 557, 630
SmoI CTYRAG 2 cut(s) 557, 630
Sse9I AATT 5 cut(s) 25, 206, 570, 863, 1164
SsiI CCGC 9 cut(s) 98, 130, 133, 151, 225, 255, 321, 372, 581
SspI AATATT 1 cut(s) 938
SspMI CTAG 1 cut(s) 1041
StyD4I CCNGG 3 cut(s) 14, 173, 220
StyI CCWWGG 1 cut(s) 963
TaaI ACNGT 4 cut(s) 217, 502, 878, 1082
TaqI TCGA 8 cut(s) 78, 204, 315, 510, 558, 631, 850, 1095
TasI AATT 5 cut(s) 25, 206, 570, 863, 1164
TauI GCSGC 4 cut(s) 101, 133, 228, 258
TfiI GAWTC 2 cut(s) 616, 716
Tru1I TTAA 2 cut(s) 723, 921
Tru9I TTAA 2 cut(s) 723, 921
TscAI CASTG 3 cut(s) 27, 874, 1087
TseFI GTSAC 1 cut(s) 18
TseI GCWGC 5 cut(s) 258, 351, 354, 381, 686
Tsp45I GTSAC 1 cut(s) 18
TspDTI ATGAA 4 cut(s) 681, 756, 786, 993
TspGWI ACGGA 2 cut(s) 131, 312
TspRI CASTG 3 cut(s) 27, 874, 1087
VneI GTGCAC 1 cut(s) 269
XapI RAATTY 2 cut(s) 863, 1164
XceI RCATGY 1 cut(s) 472
XcmI CCANNNNNNNNNTGG 1 cut(s) 1060
XhoI CTCGAG 2 cut(s) 557, 630
XspI CTAG 1 cut(s) 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.