pycom14g11670

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0013468
Physical Location & Seq
Reverse (-)
14750334 .. 14751601
1268 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 567 bp
ATGGCAACCCTACTAAAGAGAACATTTGGCTCTTTCATATCTTCCATTGCATACTACTCGTACTTTAACAACCAAGATCATCACCACCACCGTCACCACCACTACGAAAGCATCATCAACAGGAAAATCAAACACACCATGCCAAGGGGTCGGCCGCTTTCACTAGAGACTGTGGAGCTCAAAGTCAGGATGTGCTGCACTGGCTGTGAAAGAGTTGTGAAAAATGCCATTTTCAAGCTCAGAGGGATTGATTCTGTGGATGTGGATCTACCAATGGAAAAGGTGACTGTAATCGGGTACGTTGATCGCAACAAAGTCCTCAAGGCAGTGAGGAGGGCTGGAAAAAGGGCTGAGTTCTGGCCCTACCCCAACCCTCCATTGTACTTCACATCTTCAAGTGACTATTTTAAGGACACAACCAATGAATTCAAAGAGAGCTACAACTACTATAAGCATGGCTACAATGTTGGGGACAAACATGGAAATATTCCAGTGACTCAGAGGGGAGATGACAAAGTTAGCAACATGTTCAATGATGATAATGTCAATGCTTGTTGCCTTATGTAA

Protein Analysis

189

Amino Acids

21.92

Weight (kDa)

9.22

Isoelectric Point (pI)

25.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 61 - 115 3.5e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18196 AT2G18196
fragaria_vesca FvH4_5g10380 FvH4_5g10380
malus_domestica MD06G1124600.v1.1 MD14G1141600.v1.1
prunus_persica Prupe.5G135200_v2.0.a1
pyrus_communis pycom06g11500 pycom14g11670
rosa_chinensis RchiOBHm_Chr7g0187841
rosa_laevigata RLG00000004752
rosa_multiflora Rmu_sc0004483.1_g000045
rosa_roxburghii Rroxscaffold_3G00267010
rosa_rugosa Rorug06G0484700
rosa_samantha Rh7BG091600 Rh7CG090700 Rh7DG092400
rosa_wichuraiana Rw7G007760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 155
AclWI GGATC 1 cut(s) 273
AcoI YGGCCR 1 cut(s) 152
AcsI RAATTY 1 cut(s) 425
AfaI GTAC 3 cut(s) 62, 299, 383
AfiI CCNNNNNNNGG 1 cut(s) 144
AflIII ACRYGT 1 cut(s) 525
AgsI TTSAA 4 cut(s) 235, 396, 430, 532
AluBI AGCT 3 cut(s) 178, 238, 438
AluI AGCT 3 cut(s) 178, 238, 438
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 1 cut(s) 273
AoxI GGCC 2 cut(s) 152, 359
ApeKI GCWGC 1 cut(s) 195
ApoI RAATTY 1 cut(s) 425
AspS9I GGNCC 1 cut(s) 360
AsuHPI GGTGA 3 cut(s) 74, 86, 295
BanII GRGCYC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 182
BcgI CGANNNNNNTGC 2 cut(s) 39, 73
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 1 cut(s) 164
BisI GCNGC 2 cut(s) 155, 196
BlsI GCNGC 2 cut(s) 156, 197
BmgT120I GGNCC 1 cut(s) 360
BmsI GCATC 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 305
BsaBI GATNNNNATC 1 cut(s) 264
BsaJI CCNNGG 1 cut(s) 143
Bsc4I CCNNNNNNNGG 1 cut(s) 144
Bse1I ACTGG 2 cut(s) 205, 491
Bse3DI GCAATG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 143
BseGI GGATG 2 cut(s) 195, 265
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 1 cut(s) 144
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 3 cut(s) 253, 342, 512
BseNI ACTGG 2 cut(s) 205, 491
BseRI GAGGAG 1 cut(s) 346
BseX3I CGGCCG 1 cut(s) 152
BseXI GCAGC 1 cut(s) 182
BsgI GTGCAG 1 cut(s) 181
Bsh1285I CGRYCG 1 cut(s) 155
BshFI GGCC 2 cut(s) 154, 361
BsiEI CGRYCG 1 cut(s) 155
BsiHKAI GWGCWC 1 cut(s) 180
BslFI GGGAC 1 cut(s) 485
BslI CCNNNNNNNGG 1 cut(s) 144
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 485
BsnI GGCC 2 cut(s) 154, 361
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 3 cut(s) 76, 265, 304
BspACI CCGC 1 cut(s) 155
BspANI GGCC 2 cut(s) 154, 361
BspCNI CTCAG 3 cut(s) 252, 343, 511
BspPI GGATC 1 cut(s) 273
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 2 cut(s) 205, 491
BssECI CCNNGG 1 cut(s) 143
BssMI GATC 3 cut(s) 76, 265, 304
BssT1I CCWWGG 1 cut(s) 143
Bst4CI ACNGT 3 cut(s) 92, 172, 289
BstDEI CTNAG 3 cut(s) 239, 351, 498
BstF5I GGATG 2 cut(s) 195, 265
BstKTI GATC 3 cut(s) 79, 268, 307
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 3 cut(s) 76, 265, 304
BstMCI CGRYCG 1 cut(s) 155
BstMWI GCNNNNNNNGC 1 cut(s) 201
BstNSI RCATGY 1 cut(s) 529
BstV1I GCAGC 1 cut(s) 182
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BstZI CGGCCG 1 cut(s) 152
BsuRI GGCC 2 cut(s) 154, 361
BtsCI GGATG 2 cut(s) 195, 265
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 3 cut(s) 198, 333, 498
Cfr13I GGNCC 1 cut(s) 360
Csp6I GTAC 3 cut(s) 61, 298, 382
CviAII CATG 4 cut(s) 139, 455, 479, 526
CviQI GTAC 3 cut(s) 61, 298, 382
DdeI CTNAG 3 cut(s) 239, 351, 498
DpnI GATC 3 cut(s) 78, 267, 306
DpnII GATC 3 cut(s) 76, 265, 304
EaeI YGGCCR 1 cut(s) 152
EagI CGGCCG 1 cut(s) 152
Ecl136II GAGCTC 1 cut(s) 178
EclXI CGGCCG 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 180
Eco52I CGGCCG 1 cut(s) 152
Eco53kI GAGCTC 1 cut(s) 178
EcoICRI GAGCTC 1 cut(s) 178
EcoRI GAATTC 1 cut(s) 425
EcoT14I CCWWGG 1 cut(s) 143
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 143
FaeI CATG 4 cut(s) 142, 458, 482, 529
FaiI YATR 8 cut(s) 38, 52, 140, 450, 456, 480, 527, 563
FaqI GGGAC 1 cut(s) 485
FatI CATG 4 cut(s) 138, 454, 478, 525
Fnu4HI GCNGC 2 cut(s) 155, 196
FokI GGATG 2 cut(s) 202, 272
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 2 cut(s) 155, 196
FspBI CTAG 1 cut(s) 164
GluI GCNGC 2 cut(s) 155, 196
HaeIII GGCC 2 cut(s) 154, 361
Hin1II CATG 4 cut(s) 142, 458, 482, 529
HinfI GANTC 2 cut(s) 251, 496
HphI GGTGA 3 cut(s) 74, 86, 295
Hpy188I TCNGA 2 cut(s) 242, 501
Hpy188III TCNNGA 1 cut(s) 187
HpyCH4III ACNGT 3 cut(s) 92, 172, 289
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 2 cut(s) 50, 198
HpyF10VI GCNNNNNNNGC 1 cut(s) 201
HpyF3I CTNAG 3 cut(s) 239, 351, 498
HpySE526I ACGT 1 cut(s) 300
Hsp92II CATG 4 cut(s) 142, 458, 482, 529
Kzo9I GATC 3 cut(s) 76, 265, 304
LmnI GCTCC 1 cut(s) 175
LpnPI CCDG 6 cut(s) 106, 172, 186, 324, 343, 504
Lsp1109I GCAGC 1 cut(s) 182
LweI GCATC 1 cut(s) 120
MaeI CTAG 1 cut(s) 164
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 4 cut(s) 92, 283, 398, 493
MalI GATC 3 cut(s) 78, 267, 306
MboI GATC 3 cut(s) 76, 265, 304
MboII GAAGA 2 cut(s) 33, 384
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 1 cut(s) 180
MluCI AATT 1 cut(s) 425
MlyI GAGTC 1 cut(s) 490
MnlI CCTC 6 cut(s) 236, 324, 327, 329, 384, 495
MseI TTAA 2 cut(s) 66, 408
MwoI GCNNNNNNNGC 1 cut(s) 201
NdeII GATC 3 cut(s) 76, 265, 304
NlaIII CATG 4 cut(s) 142, 458, 482, 529
NmuCI GTSAC 4 cut(s) 92, 283, 398, 493
NspI RCATGY 1 cut(s) 529
PciI ACATGT 1 cut(s) 525
PfeI GAWTC 1 cut(s) 251
PkrI GCNGC 2 cut(s) 156, 197
PleI GAGTC 1 cut(s) 490
PpsI GAGTC 1 cut(s) 490
PscI ACATGT 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 180
PspPI GGNCC 1 cut(s) 360
PsuI RGATCY 1 cut(s) 265
RsaI GTAC 3 cut(s) 62, 299, 383
RsaNI GTAC 3 cut(s) 61, 298, 382
SacI GAGCTC 1 cut(s) 180
SaqAI TTAA 2 cut(s) 66, 408
SatI GCNGC 2 cut(s) 155, 196
Sau3AI GATC 3 cut(s) 76, 265, 304
Sau96I GGNCC 1 cut(s) 360
SchI GAGTC 1 cut(s) 490
SduI GDGCHC 1 cut(s) 180
SetI ASST 5 cut(s) 180, 240, 285, 303, 440
SfaNI GCATC 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 1 cut(s) 425
SsiI CCGC 1 cut(s) 155
SspI AATATT 1 cut(s) 487
SspMI CTAG 1 cut(s) 164
SstI GAGCTC 1 cut(s) 180
StyI CCWWGG 1 cut(s) 143
TaaI ACNGT 3 cut(s) 92, 172, 289
TaiI ACGT 1 cut(s) 303
TasI AATT 1 cut(s) 425
TatI WGTACW 1 cut(s) 381
TauI GCSGC 1 cut(s) 157
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 2 cut(s) 66, 408
Tru9I TTAA 2 cut(s) 66, 408
TscAI CASTG 3 cut(s) 205, 333, 498
TseFI GTSAC 4 cut(s) 92, 283, 398, 493
TseI GCWGC 1 cut(s) 195
Tsp45I GTSAC 4 cut(s) 92, 283, 398, 493
TspDTI ATGAA 2 cut(s) 25, 438
TspRI CASTG 3 cut(s) 205, 333, 498
XapI RAATTY 1 cut(s) 425
XceI RCATGY 1 cut(s) 529
XspI CTAG 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.