pycom14g13060

Ubiquitin-like modifier-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010013
Physical Location & Seq
Forward (+)
16077375 .. 16081007
3633 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1278 bp
ATGGAGGTGGAACTGAAGGAGTTATTGAGCGACCTCGAATCGCTGAAGAAATCGCTCTCCGATCCTTCTCATCATGCTCCCATCGATAAGCTACAATTGCGTGTAGAACGCCTTACGAGCCTTGCAAATCCCGGACCTGTTCGGCGTTCAAAAGTGAAGGATATGAGTGCCGAGGTTGTAGATAGCAATCCTTACAGTAGGCTTATGGCACTTCAAAGGATGGGCATTGTGGAAAACTATGAAAGAATACGGGACTTCTCAGTTGCCATTGTTGGAGTAGGTGGTGTAGGAAGTGTAGCAGCTGAGATGCTAACAAGATGTGGTATTGGTCGCCTTTTGTTGTATGATTATGACAAAGTAGAGTTAGCTAACATGAATAGGTTATTCTTTCGACCAGAGCAGAGTGGTATGACGAAAACAGATGCTGCTGTACAGACCCTTTCAGACATAAATCCCGATGTTGTGCTTGAGAGCTATACACTGAACATCACAACTGTGCAAGGTTTTGATACCTTTATGGCTAGTTTAAAAAATAAATCATTTCGCCGAAATAAAGAAGGCAGTGGAGTGGACCTTGTACTAAGCTGTGTTGATAATTATGAAGCAAGAATGGCTGTTAATCAGGCTTGCAATGAACTGAATCAGACATGGATGGAGTCTGGTGTGTCTGAGGATGCTGTTTCAGGCCATATTCAGTTGCTGATTCCTGGTGAAACTGCCTGTTTTGCATGTGCACCACCTTTGGTTGTGGCATCTGGAGTGGATGAACGTACACTTAAGCGTGAAGGGGTGTGTGCTGCATCTTTACCTACCACTATGGGAGTTGTTGCTGGGCTTCTAGTCCAAAATACACTAAAATACTTGCTGAACTTCGGAAACGTCTCCCCATACTTGGGATATAATTCTCTTAAAGACTTCTTCCCAACTATGGAAATGAAGCCAAACCCTCAGTGTTCTAATTTTGCTTGTCTGGAACGGCAGAAAGAGTACTTGCTTGTAAAGCCAGCTAGGGATGCTGCAATTAAAGCAAAGATGGAAGTTGAAGCATCATCAGTTCCGGAATCTCCACTACATCTTGATAATGAATGGGAAATAAGTGTTGTTGATGATAACGAGCCGGAAGGTACGGAGGCCAAAACTCCAGACGCTCTTCCAGAAGGTCTTGTTCGCGAGCTTCCCAGTGCAGATGAGTTCCAAAAACCACCTACTGAAGCTACAGAGGGTACAATTGACGACCTAGAAGATCTTCGAAAGCAACTTGAGGCCCTAAATTCTTGA

Protein Analysis

426

Amino Acids

46.79

Weight (kDa)

4.74

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 65 - 319 5.7e-56 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1170
AccIII TCCGGA 1 cut(s) 1057
AclWI GGATC 1 cut(s) 56
AcsI RAATTY 1 cut(s) 1270
AcuI CTGAAG 3 cut(s) 35, 65, 1230
AfaI GTAC 6 cut(s) 432, 579, 772, 989, 1126, 1225
AfiI CCNNNNNNNGG 3 cut(s) 892, 893, 928
AflII CTTAAG 1 cut(s) 776
AgsI TTSAA 3 cut(s) 150, 215, 1043
AjnI CCWGG 1 cut(s) 706
AleI CACNNNNGTG 1 cut(s) 494
AluBI AGCT 8 cut(s) 91, 302, 368, 474, 585, 1007, 1174, 1214
AluI AGCT 8 cut(s) 91, 302, 368, 474, 585, 1007, 1174, 1214
Alw21I GWGCWC 1 cut(s) 736
Alw26I GTCTC 1 cut(s) 886
Alw44I GTGCAC 1 cut(s) 732
AlwI GGATC 1 cut(s) 56
AlwNI CAGNNNCTG 2 cut(s) 425, 700
Aor13HI TCCGGA 1 cut(s) 1057
AoxI GGCC 3 cut(s) 685, 1131, 1263
ApaLI GTGCAC 1 cut(s) 732
ApeKI GCWGC 4 cut(s) 299, 425, 797, 1016
ApoI RAATTY 1 cut(s) 1270
Asp700I GAANNNNTTC 1 cut(s) 1245
AspS9I GGNCC 3 cut(s) 134, 571, 1264
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 1 cut(s) 722
AsuII TTCGAA 1 cut(s) 1249
AvaII GGWCC 2 cut(s) 134, 571
BaeGI GKGCMC 1 cut(s) 736
Bbv12I GWGCWC 1 cut(s) 736
BbvI GCAGC 4 cut(s) 311, 412, 784, 1003
BccI CCATC 4 cut(s) 89, 214, 646, 1027
BceAI ACGGC 1 cut(s) 992
BciT130I CCWGG 1 cut(s) 708
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 1 cut(s) 886
BfaI CTAG 4 cut(s) 522, 839, 1008, 1238
BfmI CTRYAG 1 cut(s) 1215
BfrI CTTAAG 1 cut(s) 776
BglII AGATCT 1 cut(s) 1243
BisI GCNGC 4 cut(s) 300, 426, 798, 1017
BlsI GCNGC 4 cut(s) 301, 427, 799, 1018
BmcAI AGTACT 1 cut(s) 989
Bme1390I CCNGG 2 cut(s) 132, 708
Bme18I GGWCC 2 cut(s) 134, 571
BmgT120I GGNCC 3 cut(s) 134, 571, 1264
BmrFI CCNGG 2 cut(s) 132, 708
BmrI ACTGGG 1 cut(s) 1173
BmsI GCATC 7 cut(s) 297, 412, 664, 761, 809, 1003, 1055
BmuI ACTGGG 1 cut(s) 1173
BpmI CTGGAG 2 cut(s) 777, 1125
Bpu14I TTCGAA 1 cut(s) 1249
BpuEI CTTGAG 1 cut(s) 488
BpuMI CCSGG 1 cut(s) 132
Bsa29I ATCGAT 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 171
BsaWI WCCGGW 1 cut(s) 1057
Bsc4I CCNNNNNNNGG 3 cut(s) 892, 893, 928
Bse1I ACTGG 1 cut(s) 1179
Bse3DI GCAATG 1 cut(s) 637
Bse8I GATNNNNATC 1 cut(s) 186
BseAI TCCGGA 1 cut(s) 1057
BseBI CCWGG 1 cut(s) 708
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 1 cut(s) 171
BseGI GGATG 5 cut(s) 225, 657, 679, 769, 1018
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 3 cut(s) 892, 893, 928
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 4 cut(s) 273, 294, 660, 962
BseNI ACTGG 1 cut(s) 1179
BseSI GKGCMC 1 cut(s) 736
BseXI GCAGC 4 cut(s) 311, 412, 784, 1003
BseYI CCCAGC 1 cut(s) 830
BsgI GTGCAG 1 cut(s) 1203
Bsh1236I CGCG 1 cut(s) 1170
BshFI GGCC 3 cut(s) 687, 1133, 1265
BshVI ATCGAT 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 736
BsiSI CCGG 3 cut(s) 132, 1058, 1118
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 3 cut(s) 892, 893, 928
BsmAI GTCTC 1 cut(s) 886
BsmBI CGTCTC 1 cut(s) 886
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 3 cut(s) 687, 1133, 1265
Bsp119I TTCGAA 1 cut(s) 1249
Bsp1286I GDGCHC 1 cut(s) 736
Bsp13I TCCGGA 1 cut(s) 1057
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 2 cut(s) 61, 1243
Bsp68I TCGCGA 1 cut(s) 1170
BspANI GGCC 3 cut(s) 687, 1133, 1265
BspCNI CTCAG 4 cut(s) 272, 295, 661, 961
BspDI ATCGAT 1 cut(s) 84
BspEI TCCGGA 1 cut(s) 1057
BspFNI CGCG 1 cut(s) 1170
BspPI GGATC 1 cut(s) 56
BspQI GCTCTTC 1 cut(s) 1155
BspT104I TTCGAA 1 cut(s) 1249
BspTI CTTAAG 1 cut(s) 776
BsrDI GCAATG 1 cut(s) 637
BsrGI TGTACA 1 cut(s) 430
BsrI ACTGG 1 cut(s) 1179
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 2 cut(s) 61, 1243
Bst2UI CCWGG 1 cut(s) 708
Bst4CI ACNGT 2 cut(s) 197, 496
Bst6I CTCTTC 1 cut(s) 1155
BstAFI CTTAAG 1 cut(s) 776
BstAUI TGTACA 1 cut(s) 430
BstBI TTCGAA 1 cut(s) 1249
BstC8I GCNNGC 3 cut(s) 628, 1005, 1172
BstDEI CTNAG 5 cut(s) 259, 303, 581, 669, 948
BstF5I GGATG 5 cut(s) 225, 657, 679, 769, 1018
BstFNI CGCG 1 cut(s) 1170
BstKTI GATC 2 cut(s) 64, 1246
BstMAI GTCTC 1 cut(s) 886
BstMBI GATC 2 cut(s) 61, 1243
BstMWI GCNNNNNNNGC 7 cut(s) 97, 117, 611, 725, 1000, 1013, 1025
BstNI CCWGG 1 cut(s) 708
BstNSI RCATGY 1 cut(s) 732
BstSCI CCNGG 2 cut(s) 130, 706
BstSFI CTRYAG 1 cut(s) 1215
BstSLI GKGCMC 1 cut(s) 736
BstUI CGCG 1 cut(s) 1170
BstV1I GCAGC 4 cut(s) 311, 412, 784, 1003
BstX2I RGATCY 1 cut(s) 1243
BstYI RGATCY 1 cut(s) 1243
Bsu15I ATCGAT 1 cut(s) 84
BsuRI GGCC 3 cut(s) 687, 1133, 1265
BsuTUI ATCGAT 1 cut(s) 84
BtsCI GGATG 5 cut(s) 225, 657, 679, 769, 1018
BtsI GCAGTG 1 cut(s) 568
BtsIMutI CAGTG 4 cut(s) 479, 568, 956, 1186
BtuMI TCGCGA 1 cut(s) 1170
Cac8I GCNNGC 3 cut(s) 628, 1005, 1172
CaiI CAGNNNCTG 2 cut(s) 425, 700
Cfr13I GGNCC 3 cut(s) 134, 571, 1264
ClaI ATCGAT 1 cut(s) 84
CseI GACGC 1 cut(s) 1154
Csp6I GTAC 6 cut(s) 431, 578, 771, 988, 1125, 1224
CviAII CATG 4 cut(s) 74, 373, 648, 729
CviQI GTAC 6 cut(s) 431, 578, 771, 988, 1125, 1224
DdeI CTNAG 5 cut(s) 259, 303, 581, 669, 948
DpnI GATC 2 cut(s) 63, 1245
DpnII GATC 2 cut(s) 61, 1243
DraI TTTAAA 1 cut(s) 528
Eam1104I CTCTTC 1 cut(s) 1155
EarI CTCTTC 1 cut(s) 1155
Eco47I GGWCC 2 cut(s) 134, 571
Eco57I CTGAAG 3 cut(s) 35, 65, 1230
EcoO109I RGGNCCY 1 cut(s) 1264
EcoRII CCWGG 1 cut(s) 706
Esp3I CGTCTC 1 cut(s) 886
FaeI CATG 4 cut(s) 77, 376, 651, 732
FaqI GGGAC 1 cut(s) 266
FatI CATG 4 cut(s) 73, 372, 647, 728
Fnu4HI GCNGC 4 cut(s) 300, 426, 798, 1017
FokI GGATG 5 cut(s) 232, 664, 686, 776, 1025
Fsp4HI GCNGC 4 cut(s) 300, 426, 798, 1017
FspBI CTAG 4 cut(s) 522, 839, 1008, 1238
GluI GCNGC 4 cut(s) 300, 426, 798, 1017
GsaI CCCAGC 1 cut(s) 834
GsuI CTGGAG 2 cut(s) 777, 1125
HaeIII GGCC 3 cut(s) 687, 1133, 1265
HapII CCGG 3 cut(s) 132, 1058, 1118
HgaI GACGC 1 cut(s) 1154
Hin1II CATG 4 cut(s) 77, 376, 651, 732
HinfI GANTC 5 cut(s) 38, 640, 656, 703, 1061
HpaII CCGG 3 cut(s) 132, 1058, 1118
HphI GGTGA 1 cut(s) 722
Hpy166II GTNNAC 3 cut(s) 571, 734, 773
Hpy188I TCNGA 5 cut(s) 61, 445, 645, 670, 875
Hpy188III TCNNGA 9 cut(s) 455, 756, 971, 1058, 1076, 1142, 1154, 1169, 1275
Hpy8I GTNNAC 3 cut(s) 571, 734, 773
HpyAV CCTTC 7 cut(s) 10, 75, 151, 551, 779, 1115, 1151
HpyCH4III ACNGT 2 cut(s) 197, 496
HpyCH4IV ACGT 2 cut(s) 769, 879
HpyCH4V TGCA 8 cut(s) 125, 499, 630, 728, 734, 800, 1019, 1184
HpyF10VI GCNNNNNNNGC 7 cut(s) 97, 117, 611, 725, 1000, 1013, 1025
HpyF3I CTNAG 5 cut(s) 259, 303, 581, 669, 948
HpySE526I ACGT 2 cut(s) 769, 879
Hsp92II CATG 4 cut(s) 77, 376, 651, 732
Kpn2I TCCGGA 1 cut(s) 1057
Kzo9I GATC 2 cut(s) 61, 1243
LguI GCTCTTC 1 cut(s) 1155
LmnI GCTCC 1 cut(s) 82
Lsp1109I GCAGC 4 cut(s) 311, 412, 784, 1003
LweI GCATC 7 cut(s) 297, 412, 664, 761, 809, 1003, 1055
MaeI CTAG 4 cut(s) 522, 839, 1008, 1238
MaeII ACGT 2 cut(s) 769, 879
MalI GATC 2 cut(s) 63, 1245
MboI GATC 2 cut(s) 61, 1243
MboII GAAGA 5 cut(s) 58, 910, 1142, 1238, 1253
MfeI CAATTG 2 cut(s) 95, 1227
MflI RGATCY 1 cut(s) 1243
MhlI GDGCHC 1 cut(s) 736
MluCI AATT 7 cut(s) 95, 595, 901, 958, 1020, 1227, 1270
MlyI GAGTC 1 cut(s) 665
MmeI TCCRAC 1 cut(s) 253
MnlI CCTC 7 cut(s) 44, 166, 664, 957, 1123, 1213, 1255
MroI TCCGGA 1 cut(s) 1057
MroXI GAANNNNTTC 1 cut(s) 1245
MseI TTAA 5 cut(s) 527, 618, 777, 909, 1023
MslI CAYNNNNRTG 1 cut(s) 494
MspA1I CMGCKG 1 cut(s) 302
MspCI CTTAAG 1 cut(s) 776
MspI CCGG 3 cut(s) 132, 1058, 1118
MspR9I CCNGG 2 cut(s) 132, 708
MunI CAATTG 2 cut(s) 95, 1227
MvaI CCWGG 1 cut(s) 708
MvnI CGCG 1 cut(s) 1170
MwoI GCNNNNNNNGC 7 cut(s) 97, 117, 611, 725, 1000, 1013, 1025
NciI CCSGG 1 cut(s) 132
NdeII GATC 2 cut(s) 61, 1243
NlaIII CATG 4 cut(s) 77, 376, 651, 732
NmeAIII GCCGAG 1 cut(s) 196
NruI TCGCGA 1 cut(s) 1170
NspI RCATGY 1 cut(s) 732
NspV TTCGAA 1 cut(s) 1249
OliI CACNNNNGTG 1 cut(s) 494
PciSI GCTCTTC 1 cut(s) 1155
PdmI GAANNNNTTC 1 cut(s) 1245
PfeI GAWTC 4 cut(s) 38, 640, 703, 1061
PfoI TCCNGGA 1 cut(s) 130
PkrI GCNGC 4 cut(s) 301, 427, 799, 1018
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
Psp6I CCWGG 1 cut(s) 706
PspFI CCCAGC 1 cut(s) 830
PspGI CCWGG 1 cut(s) 706
PspPI GGNCC 3 cut(s) 134, 571, 1264
PstNI CAGNNNCTG 2 cut(s) 425, 700
PsuI RGATCY 1 cut(s) 1243
PvuII CAGCTG 1 cut(s) 302
RruI TCGCGA 1 cut(s) 1170
RsaI GTAC 6 cut(s) 432, 579, 772, 989, 1126, 1225
RsaNI GTAC 6 cut(s) 431, 578, 771, 988, 1125, 1224
RseI CAYNNNNRTG 1 cut(s) 494
SapI GCTCTTC 1 cut(s) 1155
SaqAI TTAA 5 cut(s) 527, 618, 777, 909, 1023
SatI GCNGC 4 cut(s) 300, 426, 798, 1017
Sau3AI GATC 2 cut(s) 61, 1243
Sau96I GGNCC 3 cut(s) 134, 571, 1264
ScaI AGTACT 1 cut(s) 989
SchI GAGTC 1 cut(s) 665
ScrFI CCNGG 2 cut(s) 132, 708
SduI GDGCHC 1 cut(s) 736
SfaNI GCATC 7 cut(s) 297, 412, 664, 761, 809, 1003, 1055
SfcI CTRYAG 1 cut(s) 1215
SfuI TTCGAA 1 cut(s) 1249
SinI GGWCC 2 cut(s) 134, 571
SmiMI CAYNNNNRTG 1 cut(s) 494
SmlI CTYRAG 3 cut(s) 467, 776, 1259
SmoI CTYRAG 3 cut(s) 467, 776, 1259
Sse9I AATT 7 cut(s) 95, 595, 901, 958, 1020, 1227, 1270
SspMI CTAG 4 cut(s) 522, 839, 1008, 1238
StyD4I CCNGG 2 cut(s) 130, 706
TaaI ACNGT 2 cut(s) 197, 496
TaiI ACGT 2 cut(s) 772, 882
TaqI TCGA 4 cut(s) 36, 84, 391, 1249
TasI AATT 7 cut(s) 95, 595, 901, 958, 1020, 1227, 1270
TatI WGTACW 3 cut(s) 430, 577, 987
TfiI GAWTC 4 cut(s) 38, 640, 703, 1061
Tru1I TTAA 5 cut(s) 527, 618, 777, 909, 1023
Tru9I TTAA 5 cut(s) 527, 618, 777, 909, 1023
TscAI CASTG 4 cut(s) 486, 568, 956, 1186
TseI GCWGC 4 cut(s) 299, 425, 797, 1016
TspDTI ATGAA 7 cut(s) 255, 389, 615, 648, 780, 950, 1098
TspGWI ACGGA 1 cut(s) 1142
TspRI CASTG 4 cut(s) 486, 568, 956, 1186
Vha464I CTTAAG 1 cut(s) 776
VneI GTGCAC 1 cut(s) 732
VpaK11BI GGWCC 2 cut(s) 134, 571
XapI RAATTY 1 cut(s) 1270
XceI RCATGY 1 cut(s) 732
XmnI GAANNNNTTC 1 cut(s) 1245
XspI CTAG 4 cut(s) 522, 839, 1008, 1238
ZrmI AGTACT 1 cut(s) 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.