pycom14g14980

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007928
Physical Location & Seq
Reverse (-)
17654888 .. 17655469
582 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 582 bp
ATGAGGCTCATTGCAGTTGCCAGATCTTTTCGTGCCCGTCCCACTCTCCCTGAAACAACTGGTCCTTTGCAGTTTCGTTGCTTCCAACCTGATTTTGTGACCCTGGATCCCAATGCCAAGCCAAAAAAGTACAAATATCCGGCATTCTATGACCCGTACGGTCCTAGGCCTCCACCTTCAGATAAAATCGTTCAGCTTGCTGAGCGAATTGCAGCACTGGCCCCTGAAGCACGGCGCCAACTTGGTCCTACTCTTGCTGAGAGGCTAAGGCATCCGAAGCTGCAACCAATATCAACAGAAGGCTTGGACTTGGGTTCACAGGGAGGGGCAGCTGGTGGTTCAAAGGCCGAGGAAAAGAAGGTGGAGAAAACGGCTTTTGATGTGAAATTGGAGAAGTTCGATGCCGCTGCAAAAATCAAGGTGATCAAGGAAGTGAGGACATTTACCAATCTGGGATTGAAGGAGGCTAAGGATCTTGTCGAGAAGGTACCTTGCGTGCTTAAACAAGGGGTTACCAAAGAGGAAGCAAGTGACATAATTGAGAAAATAAAGACGGCTGGAGGAGTTGCCGTTATGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

194

Amino Acids

21.2

Weight (kDa)

9.52

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 126 - 189 6.3e-22 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 487
AccB1I GGYRCC 2 cut(s) 234, 487
AciI CCGC 1 cut(s) 405
AclWI GGATC 3 cut(s) 101, 114, 480
AcuI CTGAAG 2 cut(s) 162, 246
AcyI GRCGYC 1 cut(s) 235
AfaI GTAC 3 cut(s) 131, 158, 489
AgsI TTSAA 2 cut(s) 342, 460
AjnI CCWGG 1 cut(s) 102
AluBI AGCT 3 cut(s) 196, 280, 332
AluI AGCT 3 cut(s) 196, 280, 332
AlwI GGATC 3 cut(s) 101, 114, 480
AoxI GGCC 3 cut(s) 167, 219, 345
ApeKI GCWGC 4 cut(s) 212, 280, 329, 407
Asp718I GGTACC 1 cut(s) 487
AspA2I CCTAGG 1 cut(s) 164
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 4 cut(s) 62, 161, 220, 245
AsuHPI GGTGA 1 cut(s) 433
AvaII GGWCC 3 cut(s) 62, 161, 245
AvrII CCTAGG 1 cut(s) 164
BaeGI GKGCMC 1 cut(s) 37
BamHI GGATCC 1 cut(s) 106
BanI GGYRCC 2 cut(s) 234, 487
BbvI GCAGC 4 cut(s) 224, 267, 341, 394
BceAI ACGGC 4 cut(s) 248, 387, 554, 570
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BciT130I CCWGG 1 cut(s) 104
BclI TGATCA 1 cut(s) 423
BfaI CTAG 1 cut(s) 165
BfoI RGCGCY 1 cut(s) 238
BglII AGATCT 1 cut(s) 23
BisI GCNGC 5 cut(s) 213, 281, 330, 405, 408
BlnI CCTAGG 1 cut(s) 164
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 5 cut(s) 214, 282, 331, 406, 409
Bme1390I CCNGG 1 cut(s) 104
Bme18I GGWCC 3 cut(s) 62, 161, 245
BmgT120I GGNCC 4 cut(s) 62, 161, 220, 245
BmiI GGNNCC 4 cut(s) 108, 222, 236, 489
BmrFI CCNGG 1 cut(s) 104
BmsI GCATC 2 cut(s) 280, 391
BpmI CTGGAG 1 cut(s) 579
Bpu10I CCTNAGC 2 cut(s) 266, 468
Bpu1102I GCTNAGC 1 cut(s) 201
BsaHI GRCGYC 1 cut(s) 235
BsaJI CCNNGG 3 cut(s) 102, 164, 348
BsaXI ACNNNNNCTCC 1 cut(s) 555
Bse1I ACTGG 2 cut(s) 64, 222
Bse3DI GCAATG 1 cut(s) 9
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 3 cut(s) 102, 164, 348
BseGI GGATG 1 cut(s) 271
BseMI GCAATG 1 cut(s) 9
BseMII CTCAG 2 cut(s) 192, 249
BseNI ACTGG 2 cut(s) 64, 222
BseRI GAGGAG 1 cut(s) 576
BseSI GKGCMC 1 cut(s) 37
BseXI GCAGC 4 cut(s) 224, 267, 341, 394
BshFI GGCC 3 cut(s) 169, 221, 347
BshNI GGYRCC 2 cut(s) 234, 487
BsiSI CCGG 1 cut(s) 140
BsiWI CGTACG 1 cut(s) 156
BslFI GGGAC 1 cut(s) 24
BsmFI GGGAC 1 cut(s) 24
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 3 cut(s) 169, 221, 347
Bsp1286I GDGCHC 1 cut(s) 37
Bsp143I GATC 4 cut(s) 23, 106, 423, 472
Bsp1720I GCTNAGC 1 cut(s) 201
BspACI CCGC 1 cut(s) 405
BspANI GGCC 3 cut(s) 169, 221, 347
BspCNI CTCAG 2 cut(s) 193, 250
BspLI GGNNCC 4 cut(s) 108, 222, 236, 489
BspPI GGATC 3 cut(s) 101, 114, 480
BspT107I GGYRCC 2 cut(s) 234, 487
BsrDI GCAATG 1 cut(s) 9
BsrI ACTGG 2 cut(s) 64, 222
BssECI CCNNGG 3 cut(s) 102, 164, 348
BssMI GATC 4 cut(s) 23, 106, 423, 472
BssNI GRCGYC 1 cut(s) 235
BssT1I CCWWGG 1 cut(s) 164
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 1 cut(s) 161
BstACI GRCGYC 1 cut(s) 235
BstC8I GCNNGC 2 cut(s) 198, 497
BstDEI CTNAG 4 cut(s) 201, 258, 266, 468
BstEII GGTNACC 1 cut(s) 511
BstF5I GGATG 1 cut(s) 271
BstH2I RGCGCY 1 cut(s) 238
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 4 cut(s) 26, 109, 426, 475
BstMBI GATC 4 cut(s) 23, 106, 423, 472
BstMWI GCNNNNNNNGC 4 cut(s) 202, 218, 227, 277
BstNI CCWGG 1 cut(s) 104
BstPI GGTNACC 1 cut(s) 511
BstSCI CCNGG 1 cut(s) 102
BstSLI GKGCMC 1 cut(s) 37
BstV1I GCAGC 4 cut(s) 224, 267, 341, 394
BstX2I RGATCY 3 cut(s) 23, 106, 472
BstYI RGATCY 3 cut(s) 23, 106, 472
BsuRI GGCC 3 cut(s) 169, 221, 347
BtsCI GGATG 1 cut(s) 271
BtsIMutI CAGTG 1 cut(s) 215
Cac8I GCNNGC 2 cut(s) 198, 497
CfoI GCGC 1 cut(s) 237
Cfr13I GGNCC 4 cut(s) 62, 161, 220, 245
Csp6I GTAC 3 cut(s) 130, 157, 488
CviQI GTAC 3 cut(s) 130, 157, 488
DdeI CTNAG 4 cut(s) 201, 258, 266, 468
DinI GGCGCC 1 cut(s) 236
DpnI GATC 4 cut(s) 25, 108, 425, 474
DpnII GATC 4 cut(s) 23, 106, 423, 472
Eco130I CCWWGG 1 cut(s) 164
Eco147I AGGCCT 1 cut(s) 169
Eco47I GGWCC 3 cut(s) 62, 161, 245
Eco57I CTGAAG 2 cut(s) 162, 246
Eco91I GGTNACC 1 cut(s) 511
EcoO65I GGTNACC 1 cut(s) 511
EcoRII CCWGG 1 cut(s) 102
EcoT14I CCWWGG 1 cut(s) 164
EgeI GGCGCC 1 cut(s) 236
EheI GGCGCC 1 cut(s) 236
ErhI CCWWGG 1 cut(s) 164
FaiI YATR 3 cut(s) 150, 536, 575
FaqI GGGAC 1 cut(s) 24
FbaI TGATCA 1 cut(s) 423
Fnu4HI GCNGC 5 cut(s) 213, 281, 330, 405, 408
FokI GGATG 1 cut(s) 258
Fsp4HI GCNGC 5 cut(s) 213, 281, 330, 405, 408
FspBI CTAG 1 cut(s) 165
GlaI GCGC 1 cut(s) 236
GluI GCNGC 5 cut(s) 213, 281, 330, 405, 408
GsuI CTGGAG 1 cut(s) 579
HaeII RGCGCY 1 cut(s) 238
HaeIII GGCC 3 cut(s) 169, 221, 347
HapII CCGG 1 cut(s) 140
HhaI GCGC 1 cut(s) 237
Hin1I GRCGYC 1 cut(s) 235
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HpaII CCGG 1 cut(s) 140
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 2 cut(s) 181, 276
Hpy188III TCNNGA 1 cut(s) 481
Hpy8I GTNNAC 1 cut(s) 317
HpyAV CCTTC 5 cut(s) 186, 293, 352, 454, 478
HpyCH4III ACNGT 1 cut(s) 161
HpyCH4V TGCA 5 cut(s) 14, 70, 212, 283, 410
HpyF10VI GCNNNNNNNGC 4 cut(s) 202, 218, 227, 277
HpyF3I CTNAG 4 cut(s) 201, 258, 266, 468
Hsp92I GRCGYC 1 cut(s) 235
HspAI GCGC 1 cut(s) 235
KasI GGCGCC 1 cut(s) 234
KpnI GGTACC 1 cut(s) 491
Ksp22I TGATCA 1 cut(s) 423
Kzo9I GATC 4 cut(s) 23, 106, 423, 472
Lsp1109I GCAGC 4 cut(s) 224, 267, 341, 394
LweI GCATC 2 cut(s) 280, 391
MaeI CTAG 1 cut(s) 165
MaeIII GTNAC 3 cut(s) 97, 511, 530
MalI GATC 4 cut(s) 25, 108, 425, 474
MboI GATC 4 cut(s) 23, 106, 423, 472
MflI RGATCY 3 cut(s) 23, 106, 472
MhlI GDGCHC 1 cut(s) 37
MluCI AATT 3 cut(s) 207, 386, 537
Mly113I GGCGCC 1 cut(s) 235
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 8 cut(s) 180, 255, 317, 343, 429, 457, 514, 554
MseI TTAA 1 cut(s) 501
MspA1I CMGCKG 2 cut(s) 332, 407
MspI CCGG 1 cut(s) 140
MspR9I CCNGG 1 cut(s) 104
Mva1269I GAATGC 1 cut(s) 143
MvaI CCWGG 1 cut(s) 104
MwoI GCNNNNNNNGC 4 cut(s) 202, 218, 227, 277
NarI GGCGCC 1 cut(s) 235
NdeII GATC 4 cut(s) 23, 106, 423, 472
NlaIV GGNNCC 4 cut(s) 108, 222, 236, 489
NmeAIII GCCGAG 1 cut(s) 373
NmuCI GTSAC 2 cut(s) 97, 530
PceI AGGCCT 1 cut(s) 169
PctI GAATGC 1 cut(s) 143
Pfl23II CGTACG 1 cut(s) 156
PkrI GCNGC 5 cut(s) 214, 282, 331, 406, 409
PluTI GGCGCC 1 cut(s) 238
Psp6I CCWGG 1 cut(s) 102
PspEI GGTNACC 1 cut(s) 511
PspGI CCWGG 1 cut(s) 102
PspLI CGTACG 1 cut(s) 156
PspN4I GGNNCC 4 cut(s) 108, 222, 236, 489
PspPI GGNCC 4 cut(s) 62, 161, 220, 245
PsuI RGATCY 3 cut(s) 23, 106, 472
PvuII CAGCTG 1 cut(s) 332
RsaI GTAC 3 cut(s) 131, 158, 489
RsaNI GTAC 3 cut(s) 130, 157, 488
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 5 cut(s) 213, 281, 330, 405, 408
Sau3AI GATC 4 cut(s) 23, 106, 423, 472
Sau96I GGNCC 4 cut(s) 62, 161, 220, 245
ScrFI CCNGG 1 cut(s) 104
SduI GDGCHC 1 cut(s) 37
SetI ASST 9 cut(s) 91, 178, 198, 282, 334, 363, 423, 489, 493
SfaNI GCATC 2 cut(s) 280, 391
SfoI GGCGCC 1 cut(s) 236
SinI GGWCC 3 cut(s) 62, 161, 245
Sse9I AATT 3 cut(s) 207, 386, 537
SseBI AGGCCT 1 cut(s) 169
SsiI CCGC 1 cut(s) 405
SspDI GGCGCC 1 cut(s) 234
SspMI CTAG 1 cut(s) 165
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 1 cut(s) 102
StyI CCWWGG 1 cut(s) 164
TaaI ACNGT 1 cut(s) 161
TaqI TCGA 2 cut(s) 399, 480
TasI AATT 3 cut(s) 207, 386, 537
TatI WGTACW 1 cut(s) 129
TauI GCSGC 1 cut(s) 407
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 1 cut(s) 222
TseFI GTSAC 2 cut(s) 97, 530
TseI GCWGC 4 cut(s) 212, 280, 329, 407
Tsp45I GTSAC 2 cut(s) 97, 530
TspRI CASTG 1 cut(s) 222
VpaK11BI GGWCC 3 cut(s) 62, 161, 245
XmaJI CCTAGG 1 cut(s) 164
XspI CTAG 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.