pycom14g15490

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008341
Physical Location & Seq
Forward (+)
18109643 .. 18110411
769 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 582 bp
ATGTTCGTTAGCCGTTGCGCAGTTCAATTCTCTTTGGGGACTTTCTTCAAACACCTTCCGGTATCAAATCCGGCGACGCTCTGTCCGTACAGATTCAGACCCAAAACCCTAACCGCCGCTACAATTCGTAGCTCCTCCTCTTACTTTGAGACTTTAGATTCCCGCCAAAAAGAGCAAGTCCATCTCTATGTCGACACTCTTCTCCAATGGAACCAGAAAATGAATCTTACAGCTGTGAAGGAGGCGGATGAGGTAATGGAGAGGCACATTGAGGACTCGCTTGCAATCATACCGCCTATTAGAAATTCTTATCTCTCTCACCGTAGCCCTTCGTCCGAAAACCTCCGCCTTGTAGATGTGGGGAGCGGTGCGGGTCTTCCGGGTTTGATCTTAGCCATTGCTTGCCCAGGTAGGGAAGTGACACTGATGGAGTCTATGTACAAGAGATGCATTTTCTTGGAACACGTTGTCGGTCACATTGGTTTGTCGAATGTTCAAGTGGTAAGAGGAAGAGCAGAGGTATTGTTTCGTGTTTTTCGTAGATATAATTTTGTTTCTAGAGATGTCAAATTAAGGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GidB

Protein Analysis

194

Amino Acids

21.97

Weight (kDa)

9.6

Isoelectric Point (pI)

52.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GidB PF02527 56 - 174 1.3e-33 rRNA small subunit methyltransferase G
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 19
AccBSI CCGCTC 1 cut(s) 366
AccI GTMKAC 1 cut(s) 192
AciI CCGC 8 cut(s) 114, 117, 163, 245, 293, 346, 366, 371
AcsI RAATTY 1 cut(s) 304
AfaI GTAC 2 cut(s) 89, 440
AfiI CCNNNNNNNGG 1 cut(s) 412
AflIII ACRYGT 1 cut(s) 463
AgsI TTSAA 3 cut(s) 26, 49, 497
AhdI GACNNNNNGTC 1 cut(s) 81
AjnI CCWGG 1 cut(s) 406
AluBI AGCT 2 cut(s) 132, 233
AluI AGCT 2 cut(s) 132, 233
Alw26I GTCTC 1 cut(s) 143
ApoI RAATTY 1 cut(s) 304
AspLEI GCGC 1 cut(s) 20
AsuC2I CCSGG 1 cut(s) 381
AsuHPI GGTGA 1 cut(s) 311
BbsI GAAGAC 1 cut(s) 368
BccI CCATC 2 cut(s) 189, 421
BciT130I CCWGG 1 cut(s) 408
BcnI CCSGG 1 cut(s) 381
BcoDI GTCTC 1 cut(s) 143
BfaI CTAG 1 cut(s) 558
BisI GCNGC 1 cut(s) 117
BlsI GCNGC 1 cut(s) 118
Bme1390I CCNGG 2 cut(s) 381, 408
BmeRI GACNNNNNGTC 1 cut(s) 81
BmiI GGNNCC 1 cut(s) 212
BmrFI CCNGG 2 cut(s) 381, 408
BmsI GCATC 1 cut(s) 437
BpiI GAAGAC 1 cut(s) 368
BpuMI CCSGG 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 406
BsaWI WCCGGW 1 cut(s) 58
BsaXI ACNNNNNCTCC 2 cut(s) 422, 452
Bsc4I CCNNNNNNNGG 1 cut(s) 412
Bse3DI GCAATG 1 cut(s) 396
BseBI CCWGG 1 cut(s) 408
BseDI CCNNGG 1 cut(s) 406
BseGI GGATG 1 cut(s) 253
BseLI CCNNNNNNNGG 1 cut(s) 412
BseMI GCAATG 1 cut(s) 396
BseRI GAGGAG 2 cut(s) 124, 127
BsiSI CCGG 3 cut(s) 59, 71, 380
BslFI GGGAC 1 cut(s) 52
BslI CCNNNNNNNGG 1 cut(s) 412
BsmAI GTCTC 1 cut(s) 143
BsmFI GGGAC 1 cut(s) 52
Bsp1407I TGTACA 1 cut(s) 438
Bsp143I GATC 1 cut(s) 387
BspACI CCGC 8 cut(s) 114, 117, 163, 245, 293, 346, 366, 371
BspLI GGNNCC 1 cut(s) 212
BspQI GCTCTTC 1 cut(s) 505
BsrBI CCGCTC 1 cut(s) 366
BsrDI GCAATG 1 cut(s) 396
BsrGI TGTACA 1 cut(s) 438
BssECI CCNNGG 1 cut(s) 406
BssMI GATC 1 cut(s) 387
Bst2UI CCWGG 1 cut(s) 408
Bst4CI ACNGT 1 cut(s) 323
Bst6I CTCTTC 2 cut(s) 204, 505
BstAUI TGTACA 1 cut(s) 438
BstC8I GCNNGC 2 cut(s) 282, 403
BstDEI CTNAG 1 cut(s) 391
BstF5I GGATG 1 cut(s) 253
BstHHI GCGC 1 cut(s) 20
BstKTI GATC 1 cut(s) 390
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 1 cut(s) 387
BstNI CCWGG 1 cut(s) 408
BstSCI CCNGG 2 cut(s) 379, 406
BstV2I GAAGAC 1 cut(s) 368
BtsCI GGATG 1 cut(s) 253
BtsIMutI CAGTG 1 cut(s) 422
Cac8I GCNNGC 2 cut(s) 282, 403
CfoI GCGC 1 cut(s) 20
CseI GACGC 1 cut(s) 85
Csp6I GTAC 2 cut(s) 88, 439
CviJI RGCY 5 cut(s) 12, 132, 233, 327, 395
CviKI_1 RGCY 5 cut(s) 12, 132, 233, 327, 395
CviQI GTAC 2 cut(s) 88, 439
DdeI CTNAG 1 cut(s) 391
DpnI GATC 1 cut(s) 389
DpnII GATC 1 cut(s) 387
DriI GACNNNNNGTC 1 cut(s) 81
Eam1104I CTCTTC 2 cut(s) 204, 505
Eam1105I GACNNNNNGTC 1 cut(s) 81
EarI CTCTTC 2 cut(s) 204, 505
EciI GGCGGA 2 cut(s) 260, 335
EcoRII CCWGG 1 cut(s) 406
EcoT22I ATGCAT 1 cut(s) 452
FaiI YATR 5 cut(s) 189, 290, 437, 546, 580
FaqI GGGAC 1 cut(s) 52
FauI CCCGC 2 cut(s) 170, 364
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 1 cut(s) 117
FokI GGATG 1 cut(s) 260
Fsp4HI GCNGC 1 cut(s) 117
FspBI CTAG 1 cut(s) 558
FspI TGCGCA 1 cut(s) 19
GlaI GCGC 1 cut(s) 19
GluI GCNGC 1 cut(s) 117
HapII CCGG 3 cut(s) 59, 71, 380
HgaI GACGC 1 cut(s) 85
HhaI GCGC 1 cut(s) 20
Hin6I GCGC 1 cut(s) 18
HinP1I GCGC 1 cut(s) 18
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HinfI GANTC 5 cut(s) 93, 158, 223, 275, 431
HpaII CCGG 3 cut(s) 59, 71, 380
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 2 cut(s) 98, 337
Hpy188III TCNNGA 1 cut(s) 558
Hpy8I GTNNAC 1 cut(s) 193
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 3 cut(s) 65, 232, 339
HpyCH4III ACNGT 1 cut(s) 323
HpyCH4IV ACGT 1 cut(s) 465
HpyCH4V TGCA 2 cut(s) 284, 450
HpyF3I CTNAG 1 cut(s) 391
HpySE526I ACGT 1 cut(s) 465
HspAI GCGC 1 cut(s) 18
Kzo9I GATC 1 cut(s) 387
LguI GCTCTTC 1 cut(s) 505
LmnI GCTCC 2 cut(s) 137, 363
LpnPI CCDG 6 cut(s) 72, 84, 227, 393, 393, 420
LweI GCATC 1 cut(s) 437
MaeI CTAG 1 cut(s) 558
MaeII ACGT 1 cut(s) 465
MaeIII GTNAC 2 cut(s) 418, 473
MalI GATC 1 cut(s) 389
MbiI CCGCTC 1 cut(s) 366
MboI GATC 1 cut(s) 387
MboII GAAGA 4 cut(s) 37, 191, 368, 522
MluCI AATT 5 cut(s) 26, 123, 304, 547, 569
MlyI GAGTC 2 cut(s) 269, 440
MnlI CCTC 9 cut(s) 145, 148, 235, 244, 255, 265, 353, 500, 511
Mph1103I ATGCAT 1 cut(s) 452
MseI TTAA 1 cut(s) 572
MslI CAYNNNNRTG 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 233
MspI CCGG 3 cut(s) 59, 71, 380
MspR9I CCNGG 2 cut(s) 381, 408
MvaI CCWGG 1 cut(s) 408
NciI CCSGG 1 cut(s) 381
NdeII GATC 1 cut(s) 387
NlaIV GGNNCC 1 cut(s) 212
NmuCI GTSAC 2 cut(s) 418, 473
NsbI TGCGCA 1 cut(s) 19
NsiI ATGCAT 1 cut(s) 452
PciSI GCTCTTC 1 cut(s) 505
PcsI WCGNNNNNNNCGW 2 cut(s) 83, 535
PfeI GAWTC 3 cut(s) 93, 158, 223
PkrI GCNGC 1 cut(s) 118
PleI GAGTC 2 cut(s) 269, 439
PpsI GAGTC 2 cut(s) 269, 439
Psp6I CCWGG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 406
PspN4I GGNNCC 1 cut(s) 212
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 2 cut(s) 89, 440
RsaNI GTAC 2 cut(s) 88, 439
RseI CAYNNNNRTG 1 cut(s) 186
SalI GTCGAC 1 cut(s) 191
SapI GCTCTTC 1 cut(s) 505
SaqAI TTAA 1 cut(s) 572
SatI GCNGC 1 cut(s) 117
Sau3AI GATC 1 cut(s) 387
SchI GAGTC 2 cut(s) 269, 440
ScrFI CCNGG 2 cut(s) 381, 408
SetI ASST 8 cut(s) 57, 134, 235, 255, 345, 412, 468, 522
SfaNI GCATC 1 cut(s) 437
SmiMI CAYNNNNRTG 1 cut(s) 186
Sse9I AATT 5 cut(s) 26, 123, 304, 547, 569
SsiI CCGC 8 cut(s) 114, 117, 163, 245, 293, 346, 366, 371
SspMI CTAG 1 cut(s) 558
StyD4I CCNGG 2 cut(s) 379, 406
TaaI ACNGT 1 cut(s) 323
TaiI ACGT 1 cut(s) 468
TaqI TCGA 2 cut(s) 192, 488
TaqII GACCGA 1 cut(s) 461
TasI AATT 5 cut(s) 26, 123, 304, 547, 569
TatI WGTACW 1 cut(s) 438
TauI GCSGC 1 cut(s) 119
TfiI GAWTC 3 cut(s) 93, 158, 223
Tru1I TTAA 1 cut(s) 572
Tru9I TTAA 1 cut(s) 572
TscAI CASTG 1 cut(s) 429
TseFI GTSAC 2 cut(s) 418, 473
Tsp45I GTSAC 2 cut(s) 418, 473
TspDTI ATGAA 1 cut(s) 236
TspGWI ACGGA 1 cut(s) 75
TspRI CASTG 1 cut(s) 429
XapI RAATTY 1 cut(s) 304
XbaI TCTAGA 1 cut(s) 557
XmiI GTMKAC 1 cut(s) 192
XspI CTAG 1 cut(s) 558
Zsp2I ATGCAT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.