pycom14g15730

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011994
Physical Location & Seq
Forward (+)
18264638 .. 18265168
531 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 531 bp
ATGGTTGTTTTCGTGTTTGAAGAGCGCGAAAGCAACATGACAAAGCGATCTTTTACTCGTTATGTTGAGCAAGAGCTGGGGAGGCTGCCACATTTCGTGGTCTACGCTTTGCTCGAATGGACGCTGATCATTCTGCTCTTCGTTGACGGGTTCCTTGCGTTTGCTGCCAATGAATTCGCCAAGTTCTTCGAGCTGCAAATCCCCTGCTGGCTCTGCACCAGAATCGATCACATTCTCGTACATAGAGACCAAGATTTCTATTACAATGACTCCATTTGTGAGGCTCATAGGAAAGATGTTTCGTATCTTGCTTATTGCCACAACCACAAGAAACTGTCTGATATACGAAAAATGTGCGAGGCGTGCCTTCTTTCGTTTGCTACGGAGAAAGAATCTGACTGCGATACGTACAAGTCCTTAGTTGGGATCTTGCACAAGGATCTTGAGTGCTTCGTCGAGGATCGATCACCACCAAATTCAGCTGAGTGTGCCTGCGGCAAGGACGTGGGAGGAGGGGGCAGGGCAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

177

Amino Acids

20.31

Weight (kDa)

5.48

Isoelectric Point (pI)

60.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011994)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18990 AT1G74830
fragaria_vesca FvH4_5g16010
malus_domestica MD06G1183700.v1.1 MD14G1189800.v1.1
prunus_persica Prupe.5G182400_v2.0.a1
pyrus_communis pycom14g15730 pycom14g15740
rosa_chinensis RchiOBHm_Chr7g0180061
rosa_laevigata RLG00000005298
rosa_multiflora Rmu_sc0002219.1_g000024 Rmu_ssc0000119.1_g000026
rosa_roxburghii Rroxscaffold_3G00273160
rosa_rugosa Rorug06G0430200
rosa_samantha Rh7AG030500 Rh7BG030400 Rh7CG032200 Rh7DG031200
rosa_wichuraiana Rw7G002540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 515
AccI GTMKAC 1 cut(s) 102
AccII CGCG 1 cut(s) 27
AciI CCGC 1 cut(s) 495
AclWI GGATC 3 cut(s) 434, 447, 468
AcsI RAATTY 2 cut(s) 173, 475
AfaI GTAC 2 cut(s) 240, 410
AfiI CCNNNNNNNGG 1 cut(s) 423
AgsI TTSAA 1 cut(s) 20
AjiI CACGTC 1 cut(s) 505
AluBI AGCT 3 cut(s) 76, 193, 482
AluI AGCT 3 cut(s) 76, 193, 482
Alw26I GTCTC 1 cut(s) 240
AlwI GGATC 3 cut(s) 434, 447, 468
ApeKI GCWGC 3 cut(s) 85, 164, 193
ApoI RAATTY 2 cut(s) 173, 475
AspLEI GCGC 1 cut(s) 27
AsuHPI GGTGA 1 cut(s) 459
BbvI GCAGC 3 cut(s) 72, 151, 180
BcgI CGANNNNNNTGC 4 cut(s) 205, 239, 336, 370
BclI TGATCA 1 cut(s) 126
BcoDI GTCTC 1 cut(s) 240
BfuAI ACCTGC 1 cut(s) 515
BisI GCNGC 4 cut(s) 86, 165, 194, 496
BlsI GCNGC 4 cut(s) 87, 166, 195, 497
BmgBI CACGTC 1 cut(s) 505
BmiI GGNNCC 1 cut(s) 152
BpuEI CTTGAG 1 cut(s) 464
Bsa29I ATCGAT 2 cut(s) 225, 463
BsaAI YACGTR 1 cut(s) 408
BsaI GGTCTC 1 cut(s) 240
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 1 cut(s) 423
BseCI ATCGAT 2 cut(s) 225, 463
BseLI CCNNNNNNNGG 1 cut(s) 423
BseMII CTCAG 1 cut(s) 474
BseRI GAGGAG 1 cut(s) 525
BseXI GCAGC 3 cut(s) 72, 151, 180
BseYI CCCAGC 1 cut(s) 76
BsgI GTGCAG 1 cut(s) 199
Bsh1236I CGCG 1 cut(s) 27
BshVI ATCGAT 2 cut(s) 225, 463
BslI CCNNNNNNNGG 1 cut(s) 423
BsmAI GTCTC 1 cut(s) 240
Bso31I GGTCTC 1 cut(s) 240
Bsp143I GATC 7 cut(s) 47, 126, 226, 426, 439, 460, 464
BspACI CCGC 1 cut(s) 495
BspCNI CTCAG 1 cut(s) 475
BspDI ATCGAT 2 cut(s) 225, 463
BspFNI CGCG 1 cut(s) 27
BspLI GGNNCC 1 cut(s) 152
BspMI ACCTGC 1 cut(s) 515
BspPI GGATC 3 cut(s) 434, 447, 468
BspQI GCTCTTC 2 cut(s) 15, 143
BspTNI GGTCTC 1 cut(s) 240
BssMI GATC 7 cut(s) 47, 126, 226, 426, 439, 460, 464
Bst4CI ACNGT 1 cut(s) 336
Bst6I CTCTTC 2 cut(s) 15, 143
BstBAI YACGTR 1 cut(s) 408
BstC8I GCNNGC 3 cut(s) 209, 364, 493
BstDEI CTNAG 2 cut(s) 418, 483
BstFNI CGCG 1 cut(s) 27
BstHHI GCGC 1 cut(s) 27
BstKTI GATC 7 cut(s) 50, 129, 229, 429, 442, 463, 467
BstMAI GTCTC 1 cut(s) 240
BstMBI GATC 7 cut(s) 47, 126, 226, 426, 439, 460, 464
BstMWI GCNNNNNNNGC 5 cut(s) 82, 164, 213, 363, 488
BstSNI TACGTA 1 cut(s) 408
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 3 cut(s) 72, 151, 180
BstX2I RGATCY 2 cut(s) 426, 439
BstYI RGATCY 2 cut(s) 426, 439
Bsu15I ATCGAT 2 cut(s) 225, 463
BsuTUI ATCGAT 2 cut(s) 225, 463
BtrI CACGTC 1 cut(s) 505
BveI ACCTGC 1 cut(s) 515
Cac8I GCNNGC 3 cut(s) 209, 364, 493
CfoI GCGC 1 cut(s) 27
ClaI ATCGAT 2 cut(s) 225, 463
CseI GACGC 1 cut(s) 130
Csp6I GTAC 2 cut(s) 239, 409
CviAII CATG 1 cut(s) 37
CviJI RGCY 6 cut(s) 76, 85, 193, 211, 284, 482
CviKI_1 RGCY 6 cut(s) 76, 85, 193, 211, 284, 482
CviQI GTAC 2 cut(s) 239, 409
DdeI CTNAG 2 cut(s) 418, 483
DpnI GATC 7 cut(s) 49, 128, 228, 428, 441, 462, 466
DpnII GATC 7 cut(s) 47, 126, 226, 426, 439, 460, 464
Eam1104I CTCTTC 2 cut(s) 15, 143
EarI CTCTTC 2 cut(s) 15, 143
Eco105I TACGTA 1 cut(s) 408
Eco31I GGTCTC 1 cut(s) 240
EcoRI GAATTC 1 cut(s) 173
FaeI CATG 1 cut(s) 40
FaiI YATR 5 cut(s) 38, 63, 243, 288, 344
FatI CATG 1 cut(s) 36
FbaI TGATCA 1 cut(s) 126
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 4 cut(s) 86, 165, 194, 496
Fsp4HI GCNGC 4 cut(s) 86, 165, 194, 496
GlaI GCGC 1 cut(s) 26
GluI GCNGC 4 cut(s) 86, 165, 194, 496
GsaI CCCAGC 1 cut(s) 80
HgaI GACGC 1 cut(s) 130
HhaI GCGC 1 cut(s) 27
Hin1II CATG 1 cut(s) 40
Hin6I GCGC 1 cut(s) 25
HinP1I GCGC 1 cut(s) 25
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HinfI GANTC 3 cut(s) 222, 269, 392
HphI GGTGA 1 cut(s) 459
Hpy166II GTNNAC 2 cut(s) 103, 145
Hpy188I TCNGA 2 cut(s) 340, 397
Hpy188III TCNNGA 1 cut(s) 443
Hpy8I GTNNAC 2 cut(s) 103, 145
Hpy99I CGWCG 1 cut(s) 458
HpyAV CCTTC 1 cut(s) 377
HpyCH4III ACNGT 1 cut(s) 336
HpyCH4IV ACGT 2 cut(s) 407, 504
HpyCH4V TGCA 3 cut(s) 196, 216, 433
HpyF10VI GCNNNNNNNGC 5 cut(s) 82, 164, 213, 363, 488
HpyF3I CTNAG 2 cut(s) 418, 483
HpySE526I ACGT 2 cut(s) 407, 504
Hsp92II CATG 1 cut(s) 40
HspAI GCGC 1 cut(s) 25
Ksp22I TGATCA 1 cut(s) 126
Kzo9I GATC 7 cut(s) 47, 126, 226, 426, 439, 460, 464
LguI GCTCTTC 2 cut(s) 15, 143
LpnPI CCDG 7 cut(s) 62, 193, 217, 232, 505, 505, 510
Lsp1109I GCAGC 3 cut(s) 72, 151, 180
MaeII ACGT 2 cut(s) 407, 504
MalI GATC 7 cut(s) 49, 128, 228, 428, 441, 462, 466
MboI GATC 7 cut(s) 47, 126, 226, 426, 439, 460, 464
MboII GAAGA 3 cut(s) 32, 130, 178
MflI RGATCY 2 cut(s) 426, 439
MluCI AATT 2 cut(s) 173, 475
MlyI GAGTC 1 cut(s) 263
MnlI CCTC 6 cut(s) 75, 274, 352, 451, 503, 506
MspA1I CMGCKG 1 cut(s) 482
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 5 cut(s) 82, 164, 213, 363, 488
NdeII GATC 7 cut(s) 47, 126, 226, 426, 439, 460, 464
NlaIII CATG 1 cut(s) 40
NlaIV GGNNCC 1 cut(s) 152
PciSI GCTCTTC 2 cut(s) 15, 143
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PfeI GAWTC 2 cut(s) 222, 392
PkrI GCNGC 4 cut(s) 87, 166, 195, 497
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
Ppu21I YACGTR 1 cut(s) 408
PspFI CCCAGC 1 cut(s) 76
PspN4I GGNNCC 1 cut(s) 152
PsuI RGATCY 2 cut(s) 426, 439
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 2 cut(s) 240, 410
RsaNI GTAC 2 cut(s) 239, 409
SapI GCTCTTC 2 cut(s) 15, 143
SatI GCNGC 4 cut(s) 86, 165, 194, 496
Sau3AI GATC 7 cut(s) 47, 126, 226, 426, 439, 460, 464
SchI GAGTC 1 cut(s) 263
SetI ASST 6 cut(s) 78, 195, 410, 484, 507, 529
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
SnaBI TACGTA 1 cut(s) 408
Sse9I AATT 2 cut(s) 173, 475
SsiI CCGC 1 cut(s) 495
TaaI ACNGT 1 cut(s) 336
TaiI ACGT 2 cut(s) 410, 507
TaqI TCGA 5 cut(s) 114, 189, 225, 456, 463
TasI AATT 2 cut(s) 173, 475
TauI GCSGC 1 cut(s) 498
TfiI GAWTC 2 cut(s) 222, 392
TseI GCWGC 3 cut(s) 85, 164, 193
TspDTI ATGAA 1 cut(s) 186
TspGWI ACGGA 1 cut(s) 398
XapI RAATTY 2 cut(s) 173, 475
XmiI GTMKAC 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.