pycom14g17270

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010569
Physical Location & Seq
Forward (+)
19276487 .. 19278726
2240 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1401 bp
ATGTATATTTTTTGGATTCCAAGTGGTCCTGAGACCACCTTGGTCCCACTGTGCATCTGCCACTGCTTATACAGTCCCTATGTATGTCTCTCTTTCCCGCACCAAAATTCCCATTTCTCTGAGAGTCCCTGCAAATACCCATCACCCCGTGCAATGATAATCCCACCGGATTCCAACTTTCCTTGCGGTTCACAATCCCCAATCTTTATTCCTCCACAATATTCCAACAATTTTACCTATCAAATTCCCTATGAATCCAACCCCCCTAACCCAATTTGGTCTTTTCCTCAACCGGTGGTTCCCTCGGCAGTAGCCGCAGCGGATCCAGCCTGTCACCCGCCCGGCACCGACCCGTATGACAATTCGGGCTTGTACCCGTGGATCCGTGTCGGATTCCGAGGTCAGACGCGTTTTGGTGAAGACCCAAATATTCGTCCGCAGAACTGGCTTGCCAAGCAAGCTGAACCTGTTCGATATGAAGCTTCTTTCAAATCTTTGAATGAGAGTATGTCTGTGCCTGCAACGGCCTCGAATTCTTCGTGGAATAGCAGTTGGACAAATCAGCCTTTGGCAAATACTGCTACAATGAACACTCCACAACCAACTGTGGTTGTCCAGTCCCTGCGGTGTGATGTCTGTGATATCTATTGTACCAGCAAACCAATTTATGAGACACACATAGCGGGAAAGAAACACCAGAAGAAAGTCGCAACGCAACATAATCCCGGTGTGACTGCTGGTGCAGACTTGGAAACAAAGAGGCGTAACATTTTGAGGGGCGGTTCAACAGTTGATTCTGTGAGGGTTTGCACCATATGCAACATTACTTGCAATAGCGAAGAAGTTTATAATAAGCATCTATATGGAAAAAGGCACAAGGCTCAGGCTACCTTAATGGCTCTAGTTGGCGGGCCATATATTGCTGCAGTTGGATCTCAATTCAATGGAATATGGAAGAAAGACCCAAATAAAGTCAAGCTTGTTCAATATGCATGGTGTGAAGTTTGCAAAATCAATTGTAACAGCAACGATGCCTACGTCGCACACCTAGTTGGAAAGAAACATAAGAAGAACTTGGAGCAATTGGGAAAGTCCAAGAATGATGGGAGTGCCTCGACCTCCAATGCTCCATCAACAACAAATGCCATAATTGGGCCAACGGAAAACCTAGGAGTTGAGTCATCAAAGGCTTTCCAGCCACAGGTTCCAAAAGAAGACTTGGAGACAAAGAAGAAAAAGGTCATATCTGGAGGAGCAACAGCTAGTGCAGTCAGAACGTGTACCGTGTGCAATGTGGTGTGCAATAGCCAGACAGTTTTTTGTTCTCATCTTGTCGGTCAGAAGCACGCTGCTATGGTTAAGAAACAAACAGAAGCTGAAGTAGCAACTAGAGGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-met

Protein Analysis

467

Amino Acids

51.04

Weight (kDa)

8.93

Isoelectric Point (pI)

48.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-met PF12874 209 - 232 6.5e-07 Zinc-finger of C2H2 type
zf-met PF12874 269 - 292 3.5e-08 Zinc-finger of C2H2 type
zf-met PF12874 332 - 355 1.7e-07 Zinc-finger of C2H2 type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 849
AccB1I GGYRCC 1 cut(s) 344
AccII CGCG 1 cut(s) 409
AclWI GGATC 5 cut(s) 317, 330, 376, 389, 940
AcsI RAATTY 3 cut(s) 106, 243, 532
AcuI CTGAAG 1 cut(s) 1398
AdeI CACNNNGTG 1 cut(s) 149
AfaI GTAC 3 cut(s) 374, 652, 1282
AfiI CCNNNNNNNGG 2 cut(s) 1152, 1201
AflIII ACRYGT 2 cut(s) 407, 1277
AgeI ACCGGT 1 cut(s) 292
AgsI TTSAA 5 cut(s) 490, 499, 786, 943, 986
AluBI AGCT 5 cut(s) 461, 482, 979, 1262, 1376
AluI AGCT 5 cut(s) 461, 482, 979, 1262, 1376
Alw26I GTCTC 4 cut(s) 26, 92, 665, 1217
AlwI GGATC 5 cut(s) 317, 330, 376, 389, 940
AlwNI CAGNNNCTG 2 cut(s) 622, 1376
AoxI GGCC 4 cut(s) 525, 911, 1154, 1393
ApeKI GCWGC 3 cut(s) 317, 923, 1349
ApoI RAATTY 3 cut(s) 106, 243, 532
AsiGI ACCGGT 1 cut(s) 292
Asp700I GAANNNNTTC 1 cut(s) 468
AspA2I CCTAGG 1 cut(s) 1168
AspS9I GGNCC 4 cut(s) 26, 43, 911, 1154
AsuC2I CCSGG 2 cut(s) 342, 726
AsuHPI GGTGA 3 cut(s) 135, 326, 428
AvaII GGWCC 2 cut(s) 26, 43
AvrII CCTAGG 1 cut(s) 1168
BamHI GGATCC 2 cut(s) 322, 381
BanI GGYRCC 1 cut(s) 344
BbsI GAAGAC 2 cut(s) 426, 1221
BbvI GCAGC 3 cut(s) 329, 910, 1336
BccI CCATC 3 cut(s) 148, 1097, 1138
BceAI ACGGC 1 cut(s) 540
BcgI CGANNNNNNTGC 2 cut(s) 510, 544
BcnI CCSGG 2 cut(s) 342, 726
BcoDI GTCTC 4 cut(s) 26, 92, 665, 1217
BfaI CTAG 5 cut(s) 902, 1049, 1169, 1263, 1389
BfmI CTRYAG 1 cut(s) 924
BisI GCNGC 4 cut(s) 315, 318, 924, 1350
BlnI CCTAGG 1 cut(s) 1168
BlsI GCNGC 4 cut(s) 316, 319, 925, 1351
Bme1390I CCNGG 2 cut(s) 342, 726
Bme18I GGWCC 2 cut(s) 26, 43
BmgT120I GGNCC 4 cut(s) 26, 43, 911, 1154
BmiI GGNNCC 6 cut(s) 45, 300, 324, 346, 383, 1206
BmrFI CCNGG 2 cut(s) 342, 726
BmsI GCATC 3 cut(s) 63, 865, 1021
BpiI GAAGAC 2 cut(s) 426, 1221
BpmI CTGGAG 1 cut(s) 1269
Bpu10I CCTNAGC 1 cut(s) 882
BpuMI CCSGG 2 cut(s) 342, 726
BsaI GGTCTC 1 cut(s) 26
BsaJI CCNNGG 5 cut(s) 39, 303, 377, 397, 1168
BsaWI WCCGGW 2 cut(s) 166, 292
Bsc4I CCNNNNNNNGG 2 cut(s) 1152, 1201
Bse118I RCCGGY 1 cut(s) 292
Bse1I ACTGG 2 cut(s) 449, 616
Bse3DI GCAATG 2 cut(s) 159, 1297
BseDI CCNNGG 5 cut(s) 39, 303, 377, 397, 1168
BseLI CCNNNNNNNGG 2 cut(s) 1152, 1201
BseMI GCAATG 2 cut(s) 159, 1297
BseMII CTCAG 3 cut(s) 21, 111, 896
BseNI ACTGG 2 cut(s) 449, 616
BseRI GAGGAG 1 cut(s) 1266
BseXI GCAGC 3 cut(s) 329, 910, 1336
BsgI GTGCAG 2 cut(s) 762, 1287
Bsh1236I CGCG 1 cut(s) 409
BshFI GGCC 4 cut(s) 527, 913, 1156, 1395
BshNI GGYRCC 1 cut(s) 344
BshTI ACCGGT 1 cut(s) 292
BsiSI CCGG 4 cut(s) 167, 293, 342, 726
BslFI GGGAC 4 cut(s) 29, 60, 111, 604
BslI CCNNNNNNNGG 2 cut(s) 1152, 1201
BsmAI GTCTC 4 cut(s) 26, 92, 665, 1217
BsmFI GGGAC 4 cut(s) 29, 60, 111, 604
BsnI GGCC 4 cut(s) 527, 913, 1156, 1395
Bso31I GGTCTC 1 cut(s) 26
Bsp143I GATC 3 cut(s) 322, 381, 932
BspANI GGCC 4 cut(s) 527, 913, 1156, 1395
BspCNI CTCAG 3 cut(s) 22, 112, 895
BspFNI CGCG 1 cut(s) 409
BspLI GGNNCC 6 cut(s) 45, 300, 324, 346, 383, 1206
BspMAI CTGCAG 1 cut(s) 928
BspPI GGATC 5 cut(s) 317, 330, 376, 389, 940
BspT107I GGYRCC 1 cut(s) 344
BspTNI GGTCTC 1 cut(s) 26
BsrDI GCAATG 2 cut(s) 159, 1297
BsrFI RCCGGY 1 cut(s) 292
BsrI ACTGG 2 cut(s) 449, 616
BssAI RCCGGY 1 cut(s) 292
BssECI CCNNGG 5 cut(s) 39, 303, 377, 397, 1168
BssMI GATC 3 cut(s) 322, 381, 932
BssT1I CCWWGG 2 cut(s) 39, 1168
Bst4CI ACNGT 6 cut(s) 51, 74, 607, 790, 1285, 1315
BstAPI GCANNNNNTGC 2 cut(s) 578, 816
BstC8I GCNNGC 5 cut(s) 450, 459, 519, 911, 1347
BstDEI CTNAG 3 cut(s) 30, 120, 882
BstDSI CCRYGG 1 cut(s) 377
BstFNI CGCG 1 cut(s) 409
BstKTI GATC 3 cut(s) 325, 384, 935
BstMAI GTCTC 4 cut(s) 26, 92, 665, 1217
BstMBI GATC 3 cut(s) 322, 381, 932
BstMWI GCNNNNNNNGC 9 cut(s) 314, 326, 445, 454, 458, 578, 816, 1040, 1382
BstSCI CCNGG 2 cut(s) 340, 724
BstSFI CTRYAG 1 cut(s) 924
BstUI CGCG 1 cut(s) 409
BstV1I GCAGC 3 cut(s) 329, 910, 1336
BstV2I GAAGAC 2 cut(s) 426, 1221
BstX2I RGATCY 3 cut(s) 322, 381, 932
BstYI RGATCY 3 cut(s) 322, 381, 932
BsuRI GGCC 4 cut(s) 527, 913, 1156, 1395
BtgI CCRYGG 1 cut(s) 377
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 2 cut(s) 47, 61
Cac8I GCNNGC 5 cut(s) 450, 459, 519, 911, 1347
CaiI CAGNNNCTG 2 cut(s) 622, 1376
Cfr10I RCCGGY 1 cut(s) 292
Cfr13I GGNCC 4 cut(s) 26, 43, 911, 1154
CseI GACGC 1 cut(s) 415
Csp6I GTAC 3 cut(s) 373, 651, 1281
CspAI ACCGGT 1 cut(s) 292
CviAII CATG 1 cut(s) 993
CviQI GTAC 3 cut(s) 373, 651, 1281
DdeI CTNAG 3 cut(s) 30, 120, 882
DpnI GATC 3 cut(s) 324, 383, 934
DpnII GATC 3 cut(s) 322, 381, 932
DraIII CACNNNGTG 1 cut(s) 149
Eco130I CCWWGG 2 cut(s) 39, 1168
Eco31I GGTCTC 1 cut(s) 26
Eco32I GATATC 1 cut(s) 643
Eco47I GGWCC 2 cut(s) 26, 43
Eco57I CTGAAG 1 cut(s) 1398
EcoRI GAATTC 1 cut(s) 532
EcoRV GATATC 1 cut(s) 643
EcoT14I CCWWGG 2 cut(s) 39, 1168
EcoT22I ATGCAT 1 cut(s) 994
ErhI CCWWGG 2 cut(s) 39, 1168
FaeI CATG 1 cut(s) 996
FalI AAGNNNNNCTT 4 cut(s) 963, 995, 1058, 1090
FaqI GGGAC 4 cut(s) 29, 60, 111, 604
FatI CATG 1 cut(s) 992
FauI CCCGC 4 cut(s) 105, 345, 676, 902
FauNDI CATATG 1 cut(s) 815
Fnu4HI GCNGC 4 cut(s) 315, 318, 924, 1350
Fsp4HI GCNGC 4 cut(s) 315, 318, 924, 1350
FspBI CTAG 5 cut(s) 902, 1049, 1169, 1263, 1389
GluI GCNGC 4 cut(s) 315, 318, 924, 1350
GsuI CTGGAG 1 cut(s) 1269
HaeIII GGCC 4 cut(s) 527, 913, 1156, 1395
HapII CCGG 4 cut(s) 167, 293, 342, 726
HgaI GACGC 1 cut(s) 415
Hin1II CATG 1 cut(s) 996
HindIII AAGCTT 2 cut(s) 480, 977
HinfI GANTC 7 cut(s) 16, 124, 170, 254, 393, 794, 1178
HpaII CCGG 4 cut(s) 167, 293, 342, 726
HphI GGTGA 3 cut(s) 135, 326, 428
Hpy166II GTNNAC 2 cut(s) 191, 1281
Hpy188I TCNGA 6 cut(s) 121, 392, 398, 405, 1274, 1341
Hpy188III TCNNGA 2 cut(s) 29, 1248
Hpy8I GTNNAC 2 cut(s) 191, 1281
Hpy99I CGWCG 1 cut(s) 1043
HpyCH4III ACNGT 6 cut(s) 51, 74, 607, 790, 1285, 1315
HpyCH4IV ACGT 2 cut(s) 1038, 1277
HpyF10VI GCNNNNNNNGC 9 cut(s) 314, 326, 445, 454, 458, 578, 816, 1040, 1382
HpyF3I CTNAG 3 cut(s) 30, 120, 882
HpySE526I ACGT 2 cut(s) 1038, 1277
Hsp92II CATG 1 cut(s) 996
Kzo9I GATC 3 cut(s) 322, 381, 932
LmnI GCTCC 3 cut(s) 1078, 1132, 1253
Lsp1109I GCAGC 3 cut(s) 329, 910, 1336
LweI GCATC 3 cut(s) 63, 865, 1021
MaeI CTAG 5 cut(s) 902, 1049, 1169, 1263, 1389
MaeII ACGT 2 cut(s) 1038, 1277
MaeIII GTNAC 4 cut(s) 332, 730, 764, 1019
MalI GATC 3 cut(s) 324, 383, 934
MboI GATC 3 cut(s) 322, 381, 932
MboII GAAGA 8 cut(s) 431, 528, 712, 851, 967, 1081, 1226, 1243
MfeI CAATTG 2 cut(s) 1015, 1082
MflI RGATCY 3 cut(s) 322, 381, 932
MluI ACGCGT 1 cut(s) 407
MlyI GAGTC 2 cut(s) 133, 1187
MmeI TCCRAC 7 cut(s) 198, 249, 282, 370, 533, 910, 1033
Mph1103I ATGCAT 1 cut(s) 994
MroXI GAANNNNTTC 1 cut(s) 468
MseI TTAA 2 cut(s) 893, 1359
MslI CAYNNNNRTG 1 cut(s) 861
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 4 cut(s) 167, 293, 342, 726
MspR9I CCNGG 2 cut(s) 342, 726
MunI CAATTG 2 cut(s) 1015, 1082
MvnI CGCG 1 cut(s) 409
MwoI GCNNNNNNNGC 9 cut(s) 314, 326, 445, 454, 458, 578, 816, 1040, 1382
NciI CCSGG 2 cut(s) 342, 726
NdeI CATATG 1 cut(s) 815
NdeII GATC 3 cut(s) 322, 381, 932
NlaIII CATG 1 cut(s) 996
NlaIV GGNNCC 6 cut(s) 45, 300, 324, 346, 383, 1206
NmeAIII GCCGAG 1 cut(s) 284
NmuCI GTSAC 2 cut(s) 332, 730
NsiI ATGCAT 1 cut(s) 994
PcsI WCGNNNNNNNCGW 2 cut(s) 536, 1035
PdmI GAANNNNTTC 1 cut(s) 468
PfeI GAWTC 5 cut(s) 16, 170, 254, 393, 794
PinAI ACCGGT 1 cut(s) 292
PkrI GCNGC 4 cut(s) 316, 319, 925, 1351
PleI GAGTC 2 cut(s) 132, 1186
PpsI GAGTC 2 cut(s) 132, 1186
PsiI TTATAA 1 cut(s) 849
PspN4I GGNNCC 6 cut(s) 45, 300, 324, 346, 383, 1206
PspPI GGNCC 4 cut(s) 26, 43, 911, 1154
PstI CTGCAG 1 cut(s) 928
PstNI CAGNNNCTG 2 cut(s) 622, 1376
PsuI RGATCY 3 cut(s) 322, 381, 932
RsaI GTAC 3 cut(s) 374, 652, 1282
RsaNI GTAC 3 cut(s) 373, 651, 1281
RseI CAYNNNNRTG 1 cut(s) 861
SaqAI TTAA 2 cut(s) 893, 1359
SatI GCNGC 4 cut(s) 315, 318, 924, 1350
Sau3AI GATC 3 cut(s) 322, 381, 932
Sau96I GGNCC 4 cut(s) 26, 43, 911, 1154
SchI GAGTC 2 cut(s) 133, 1187
ScrFI CCNGG 2 cut(s) 342, 726
SfaNI GCATC 3 cut(s) 63, 865, 1021
SfcI CTRYAG 1 cut(s) 924
SinI GGWCC 2 cut(s) 26, 43
SmiMI CAYNNNNRTG 1 cut(s) 861
SspI AATATT 2 cut(s) 221, 430
SspMI CTAG 5 cut(s) 902, 1049, 1169, 1263, 1389
StyD4I CCNGG 2 cut(s) 340, 724
StyI CCWWGG 2 cut(s) 39, 1168
TaaI ACNGT 6 cut(s) 51, 74, 607, 790, 1285, 1315
TaiI ACGT 2 cut(s) 1041, 1280
TaqI TCGA 3 cut(s) 472, 530, 1115
TaqII GACCGA 1 cut(s) 1325
TauI GCSGC 1 cut(s) 317
TfiI GAWTC 5 cut(s) 16, 170, 254, 393, 794
Tru1I TTAA 2 cut(s) 893, 1359
Tru9I TTAA 2 cut(s) 893, 1359
TscAI CASTG 2 cut(s) 54, 68
TseFI GTSAC 2 cut(s) 332, 730
TseI GCWGC 3 cut(s) 317, 923, 1349
Tsp45I GTSAC 2 cut(s) 332, 730
TspDTI ATGAA 3 cut(s) 267, 492, 602
TspGWI ACGGA 2 cut(s) 374, 1175
TspRI CASTG 2 cut(s) 54, 68
VpaK11BI GGWCC 2 cut(s) 26, 43
XapI RAATTY 3 cut(s) 106, 243, 532
XmaJI CCTAGG 1 cut(s) 1168
XmnI GAANNNNTTC 1 cut(s) 468
XspI CTAG 5 cut(s) 902, 1049, 1169, 1263, 1389
Zsp2I ATGCAT 1 cut(s) 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.