pycom14g17430

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008271
Physical Location & Seq
Forward (+)
19370583 .. 19371410
828 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 336 bp
ATGGCTGCCCTTGCTTTTGCAGCTCTGCTTCTCTTAGCCATGGCTGGCCAGTCAAGAGCAAACTGGTGTGTTTGCAAAGATGGAGACCCTGCAGCCCTGCAGAAGGCACTGGACTATGCCTGTGGAGCCGGGGCAGATTGCAACCCCATAAAACCCAATGGAGTTTGCTACAACCCCAACACTGTCAAAGCTCACTGCAGCTACGCCGTCAACAGCTTTTTCCAGAAGAAGGGCCAATCCACCGGTACTTGTGACTTTTCTGGCGCTGCAACCCCTACTACCTCCGATCCTAGCTCAAATGGTTGTACTTACCCTGCCAGTGCCAGGTATGGTTAA

Protein Analysis

112

Amino Acids

11.38

Weight (kDa)

8.12

Isoelectric Point (pI)

30.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 22 - 91 5.1e-22 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 281
AcoI YGGCCR 1 cut(s) 46
AfaI GTAC 2 cut(s) 247, 307
AfiI CCNNNNNNNGG 3 cut(s) 103, 229, 324
AgeI ACCGGT 1 cut(s) 242
AjnI CCWGG 1 cut(s) 323
AluBI AGCT 5 cut(s) 23, 191, 201, 216, 294
AluI AGCT 5 cut(s) 23, 191, 201, 216, 294
Alw26I GTCTC 1 cut(s) 78
AlwI GGATC 1 cut(s) 281
AoxI GGCC 2 cut(s) 46, 232
ApeKI GCWGC 5 cut(s) 5, 20, 92, 198, 266
AsiGI ACCGGT 1 cut(s) 242
AspLEI GCGC 1 cut(s) 266
AspS9I GGNCC 1 cut(s) 232
AsuC2I CCSGG 1 cut(s) 130
BalI TGGCCA 1 cut(s) 48
BbvI GCAGC 4 cut(s) 32, 104, 210, 253
BccI CCATC 1 cut(s) 74
BceAI ACGGC 1 cut(s) 191
BciT130I CCWGG 1 cut(s) 325
BcnI CCSGG 1 cut(s) 130
BcoDI GTCTC 1 cut(s) 78
BfaI CTAG 1 cut(s) 291
BfmI CTRYAG 3 cut(s) 90, 98, 196
BfoI RGCGCY 1 cut(s) 267
BisI GCNGC 5 cut(s) 6, 21, 93, 199, 267
BlsI GCNGC 5 cut(s) 7, 22, 94, 200, 268
Bme1390I CCNGG 2 cut(s) 130, 325
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 1 cut(s) 127
BmrFI CCNGG 2 cut(s) 130, 325
BpuMI CCSGG 1 cut(s) 130
BsaI GGTCTC 1 cut(s) 78
BsaJI CCNNGG 2 cut(s) 39, 129
BsaWI WCCGGW 1 cut(s) 242
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 229, 324
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 4 cut(s) 49, 68, 114, 318
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 2 cut(s) 39, 129
BseLI CCNNNNNNNGG 3 cut(s) 103, 229, 324
BseNI ACTGG 4 cut(s) 49, 68, 114, 318
BseXI GCAGC 4 cut(s) 32, 104, 210, 253
BshFI GGCC 2 cut(s) 48, 234
BshTI ACCGGT 1 cut(s) 242
BsiSI CCGG 2 cut(s) 129, 243
BslI CCNNNNNNNGG 3 cut(s) 103, 229, 324
BsmAI GTCTC 1 cut(s) 78
BsnI GGCC 2 cut(s) 48, 234
Bso31I GGTCTC 1 cut(s) 78
Bsp143I GATC 1 cut(s) 286
Bsp19I CCATGG 1 cut(s) 39
BspANI GGCC 2 cut(s) 48, 234
BspLI GGNNCC 1 cut(s) 127
BspMAI CTGCAG 3 cut(s) 94, 102, 200
BspPI GGATC 1 cut(s) 281
BspTNI GGTCTC 1 cut(s) 78
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 4 cut(s) 49, 68, 114, 318
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 2 cut(s) 39, 129
BssMI GATC 1 cut(s) 286
BssT1I CCWWGG 1 cut(s) 39
Bst2UI CCWGG 1 cut(s) 325
Bst4CI ACNGT 1 cut(s) 184
BstC8I GCNNGC 1 cut(s) 46
BstDEI CTNAG 1 cut(s) 34
BstDSI CCRYGG 1 cut(s) 39
BstENI CCTNNNNNAGG 1 cut(s) 101
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 1 cut(s) 266
BstKTI GATC 1 cut(s) 289
BstMAI GTCTC 1 cut(s) 78
BstMBI GATC 1 cut(s) 286
BstMWI GCNNNNNNNGC 3 cut(s) 11, 20, 125
BstNI CCWGG 1 cut(s) 325
BstSCI CCNGG 2 cut(s) 128, 323
BstSFI CTRYAG 3 cut(s) 90, 98, 196
BstV1I GCAGC 4 cut(s) 32, 104, 210, 253
BsuRI GGCC 2 cut(s) 48, 234
BtgI CCRYGG 1 cut(s) 39
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 4 cut(s) 107, 180, 193, 325
Cac8I GCNNGC 1 cut(s) 46
CfoI GCGC 1 cut(s) 266
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 1 cut(s) 232
Csp6I GTAC 2 cut(s) 246, 306
CspAI ACCGGT 1 cut(s) 242
CviAII CATG 1 cut(s) 40
CviQI GTAC 2 cut(s) 246, 306
DdeI CTNAG 1 cut(s) 34
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
EaeI YGGCCR 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 78
EcoNI CCTNNNNNAGG 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 323
EcoT14I CCWWGG 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 1 cut(s) 43
FaiI YATR 4 cut(s) 41, 117, 149, 330
FatI CATG 1 cut(s) 39
Fnu4HI GCNGC 5 cut(s) 6, 21, 93, 199, 267
Fsp4HI GCNGC 5 cut(s) 6, 21, 93, 199, 267
FspBI CTAG 1 cut(s) 291
GlaI GCGC 1 cut(s) 265
GluI GCNGC 5 cut(s) 6, 21, 93, 199, 267
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 2 cut(s) 48, 234
HapII CCGG 2 cut(s) 129, 243
HhaI GCGC 1 cut(s) 266
Hin1II CATG 1 cut(s) 43
Hin6I GCGC 1 cut(s) 264
HinP1I GCGC 1 cut(s) 264
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HpaII CCGG 2 cut(s) 129, 243
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 1 cut(s) 286
Hpy188III TCNNGA 2 cut(s) 54, 223
Hpy8I GTNNAC 1 cut(s) 211
HpyAV CCTTC 2 cut(s) 97, 223
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4V TGCA 7 cut(s) 20, 75, 92, 100, 141, 198, 269
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 20, 125
HpyF3I CTNAG 1 cut(s) 34
Hsp92II CATG 1 cut(s) 43
HspAI GCGC 1 cut(s) 264
Kzo9I GATC 1 cut(s) 286
LmnI GCTCC 1 cut(s) 125
Lsp1109I GCAGC 4 cut(s) 32, 104, 210, 253
MaeI CTAG 1 cut(s) 291
MaeIII GTNAC 1 cut(s) 251
MalI GATC 1 cut(s) 288
MboI GATC 1 cut(s) 286
MboII GAAGA 1 cut(s) 238
MlsI TGGCCA 1 cut(s) 48
MluNI TGGCCA 1 cut(s) 48
MnlI CCTC 1 cut(s) 292
Mox20I TGGCCA 1 cut(s) 48
MscI TGGCCA 1 cut(s) 48
MseI TTAA 1 cut(s) 334
Msp20I TGGCCA 1 cut(s) 48
MspI CCGG 2 cut(s) 129, 243
MspR9I CCNGG 2 cut(s) 130, 325
MvaI CCWGG 1 cut(s) 325
MwoI GCNNNNNNNGC 3 cut(s) 11, 20, 125
NciI CCSGG 1 cut(s) 130
NcoI CCATGG 1 cut(s) 39
NdeII GATC 1 cut(s) 286
NlaIII CATG 1 cut(s) 43
NlaIV GGNNCC 1 cut(s) 127
NmuCI GTSAC 1 cut(s) 251
PinAI ACCGGT 1 cut(s) 242
PkrI GCNGC 5 cut(s) 7, 22, 94, 200, 268
Psp6I CCWGG 1 cut(s) 323
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 1 cut(s) 127
PspPI GGNCC 1 cut(s) 232
PstI CTGCAG 3 cut(s) 94, 102, 200
RsaI GTAC 2 cut(s) 247, 307
RsaNI GTAC 2 cut(s) 246, 306
SaqAI TTAA 1 cut(s) 334
SatI GCNGC 5 cut(s) 6, 21, 93, 199, 267
Sau3AI GATC 1 cut(s) 286
Sau96I GGNCC 1 cut(s) 232
ScrFI CCNGG 2 cut(s) 130, 325
SetI ASST 7 cut(s) 25, 193, 203, 218, 284, 296, 329
SfcI CTRYAG 3 cut(s) 90, 98, 196
SspMI CTAG 1 cut(s) 291
StyD4I CCNGG 2 cut(s) 128, 323
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 1 cut(s) 184
TatI WGTACW 1 cut(s) 305
Tru1I TTAA 1 cut(s) 334
Tru9I TTAA 1 cut(s) 334
TscAI CASTG 4 cut(s) 114, 187, 200, 325
TseFI GTSAC 1 cut(s) 251
TseI GCWGC 5 cut(s) 5, 20, 92, 198, 266
Tsp45I GTSAC 1 cut(s) 251
TspRI CASTG 4 cut(s) 114, 187, 200, 325
XagI CCTNNNNNAGG 1 cut(s) 101
XspI CTAG 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.