pycom14g17960

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0015444
Physical Location & Seq
Reverse (-)
19746867 .. 19747838
972 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 972 bp
ATGCAAGACATTCATTCAGTGAGAGGAGGAGGGAGGTTCTTCGGCGGCTCGGGAGGAGGAGACCGGCGGCTGAGGCCGCACCAGCACCCCAACCAGCAGGCTTTGAAGTGCCCGCGGTGCGATTCTCTCCACACCAAGTTCTGCTACTACAACAACTACAACCTCTCCCAGCCCCGCCACTTCTGTAAGGCCTGCCGCCGCTACTGGACCAAAGGCGGCGTCCTCCGTAACGTCCCAGTCGGCGGTGGATGTCGCAAAACTAAACGCTCCAAGCCGAAAAATTCATCTTCGTCATCGCCGACATCGTCTCCCTCTCCTCCGCCGCCGCAACCGAACAGCGCCCATCAGAACAAATCCAGCTCTCATTCGAGCAGCGAGAGCTCGAGCCTCACCAACCCCACCGCCGCCGCGGAGGCCGTCTCAGCGCCGTCTTCGACCGGCTCTGCTTCCAATTTACTGACCAATATTCATAATCCGGAATCCAAATTCTTCATTTGTCAAGGCGGCGCGAACGGTGGCTTTGAGCCCGGCGTGTCGGCGGCGGCGCTGCTGGAGCAGAGTACGGAGATTAACGGAATGTCTTCCGAGATTGGGAGCTTCACGAGCTTGATCACGTCTTCCAACGACGTGCCGTTTAGTTTCAGGAACATCAACGGCTTGCCGTTTGATCAGCAAGACCAAGTTGGTAATCATCATCACAATCAAGTTCATCAAAACCAATGGGGGCAGCAGAACCAGGAAGTGAAGATGCAGGAGATCAGTGGTGGATTGCTGGATCAGACGGCGCAGGTCGATCTATCAGTGTTCCAAAACAGATCCAACGGCGTCGGAGGAGGAGAATTTGGACCGTTGGATTGGCAACCGGGATCAGTTGATCAAGGTTTTTTTGATCTTCCTAACACCGTTGATCAAGCATACTGGAGTCAAAGTCAATGGGCTGATCAAGACCACCCTACTCTCTACCTCCCGTAA

Protein Analysis

324

Amino Acids

35.0

Weight (kDa)

7.7

Isoelectric Point (pI)

62.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 33 - 89 2.4e-33 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60850
fragaria_vesca FvH4_5g13390
malus_domestica MD06G1205900.v1.1 MD14G1216800.v1.1
prunus_persica Prupe.5G210200_v2.0.a1
pyrus_communis pycom06g18370 pycom14g17960
rosa_chinensis RchiOBHm_Chr7g0183751
rosa_laevigata RLG00000005026
rosa_multiflora Rmu_sc0003610.1_g000031
rosa_rugosa Rorug06G0460000
rosa_samantha Rh7AG060700 Rh7BG060800 Rh7CG062400 Rh7DG060800
rosa_wichuraiana Rw7G005040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 778
AccII CGCG 3 cut(s) 115, 410, 509
AccIII TCCGGA 1 cut(s) 475
AclWI GGATC 3 cut(s) 783, 810, 874
AcsI RAATTY 3 cut(s) 280, 485, 839
AcyI GRCGYC 2 cut(s) 219, 825
AfaI GTAC 1 cut(s) 562
AfiI CCNNNNNNNGG 2 cut(s) 242, 591
AgsI TTSAA 1 cut(s) 106
AjiI CACGTC 2 cut(s) 615, 628
AjnI CCWGG 1 cut(s) 735
AluBI AGCT 4 cut(s) 360, 381, 597, 606
AluI AGCT 4 cut(s) 360, 381, 597, 606
Alw21I GWGCWC 1 cut(s) 383
Alw26I GTCTC 3 cut(s) 54, 312, 424
AlwI GGATC 3 cut(s) 783, 810, 874
Ama87I CYCGRG 2 cut(s) 49, 382
Aor13HI TCCGGA 1 cut(s) 475
AoxI GGCC 3 cut(s) 74, 189, 414
ApeKI GCWGC 3 cut(s) 372, 547, 727
ApoI RAATTY 3 cut(s) 280, 485, 839
ArsI GACNNNNNNTTYG 2 cut(s) 204, 236
Asp700I GAANNNNTTC 1 cut(s) 580
AspLEI GCGC 5 cut(s) 341, 427, 509, 547, 787
AspS9I GGNCC 2 cut(s) 207, 845
AsuC2I CCSGG 2 cut(s) 528, 864
AsuHPI GGTGA 1 cut(s) 382
AvaI CYCGRG 2 cut(s) 49, 382
AvaII GGWCC 2 cut(s) 207, 845
BaeGI GKGCMC 1 cut(s) 113
BanII GRGCYC 2 cut(s) 383, 528
BauI CACGAG 1 cut(s) 601
BbsI GAAGAC 3 cut(s) 423, 573, 609
Bbv12I GWGCWC 1 cut(s) 383
BbvCI CCTCAGC 1 cut(s) 71
BbvI GCAGC 3 cut(s) 384, 534, 739
BccI CCATC 1 cut(s) 351
BceAI ACGGC 7 cut(s) 401, 412, 616, 646, 670, 798, 838
BciT130I CCWGG 1 cut(s) 737
BclI TGATCA 5 cut(s) 609, 667, 874, 907, 940
BcnI CCSGG 2 cut(s) 528, 864
BcoDI GTCTC 3 cut(s) 54, 312, 424
BfoI RGCGCY 3 cut(s) 342, 428, 548
BfuAI ACCTGC 1 cut(s) 778
BglI GCCNNNNNGGC 1 cut(s) 413
Bme1390I CCNGG 3 cut(s) 528, 737, 864
Bme18I GGWCC 2 cut(s) 207, 845
BmeT110I CYCGRG 2 cut(s) 49, 382
BmgBI CACGTC 2 cut(s) 615, 628
BmgT120I GGNCC 2 cut(s) 207, 845
BmrFI CCNGG 3 cut(s) 528, 737, 864
BmrI ACTGGG 1 cut(s) 230
BmsI GCATC 1 cut(s) 738
BmuI ACTGGG 1 cut(s) 230
BpiI GAAGAC 3 cut(s) 423, 573, 609
BplI GAGNNNNNCTC 2 cut(s) 404, 436
BpmI CTGGAG 2 cut(s) 572, 940
Bpu10I CCTNAGC 1 cut(s) 71
BpuMI CCSGG 2 cut(s) 528, 864
BsaHI GRCGYC 2 cut(s) 219, 825
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 2 cut(s) 113, 408
BsaWI WCCGGW 1 cut(s) 475
BsaXI ACNNNNNCTCC 6 cut(s) 149, 179, 292, 322, 913, 943
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 591
Bse118I RCCGGY 2 cut(s) 63, 437
Bse1I ACTGG 3 cut(s) 209, 236, 923
BseAI TCCGGA 1 cut(s) 475
BseBI CCWGG 1 cut(s) 737
BseDI CCNNGG 2 cut(s) 113, 408
BseGI GGATG 1 cut(s) 254
BseLI CCNNNNNNNGG 2 cut(s) 242, 591
BseMII CTCAG 2 cut(s) 62, 435
BseNI ACTGG 3 cut(s) 209, 236, 923
BseRI GAGGAG 7 cut(s) 39, 42, 69, 72, 306, 846, 849
BseSI GKGCMC 1 cut(s) 113
BseXI GCAGC 3 cut(s) 384, 534, 739
BseYI CCCAGC 1 cut(s) 168
Bsh1236I CGCG 3 cut(s) 115, 410, 509
Bsh1285I CGRYCG 1 cut(s) 438
BshFI GGCC 3 cut(s) 76, 191, 416
BsiEI CGRYCG 1 cut(s) 438
BsiHKAI GWGCWC 1 cut(s) 383
BsiHKCI CYCGRG 2 cut(s) 49, 382
BsiSI CCGG 5 cut(s) 64, 438, 476, 528, 863
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 2 cut(s) 242, 591
BsmAI GTCTC 3 cut(s) 54, 312, 424
BsmBI CGTCTC 2 cut(s) 312, 424
BsmFI GGGAC 1 cut(s) 218
BsnI GGCC 3 cut(s) 76, 191, 416
Bso31I GGTCTC 1 cut(s) 54
BsoBI CYCGRG 2 cut(s) 49, 382
Bsp1286I GDGCHC 3 cut(s) 113, 383, 528
Bsp13I TCCGGA 1 cut(s) 475
BspANI GGCC 3 cut(s) 76, 191, 416
BspCNI CTCAG 2 cut(s) 63, 434
BspEI TCCGGA 1 cut(s) 475
BspFNI CGCG 3 cut(s) 115, 410, 509
BspMI ACCTGC 1 cut(s) 778
BspPI GGATC 3 cut(s) 783, 810, 874
BspTNI GGTCTC 1 cut(s) 54
BsrFI RCCGGY 2 cut(s) 63, 437
BsrI ACTGG 3 cut(s) 209, 236, 923
BssAI RCCGGY 2 cut(s) 63, 437
BssECI CCNNGG 2 cut(s) 113, 408
BssNI GRCGYC 2 cut(s) 219, 825
BssSI CACGAG 1 cut(s) 601
Bst2BI CACGAG 1 cut(s) 601
Bst2UI CCWGG 1 cut(s) 737
Bst4CI ACNGT 3 cut(s) 515, 849, 904
BstACI GRCGYC 2 cut(s) 219, 825
BstC8I GCNNGC 4 cut(s) 99, 113, 193, 659
BstDEI CTNAG 2 cut(s) 71, 421
BstDSI CCRYGG 2 cut(s) 113, 408
BstF5I GGATG 1 cut(s) 254
BstFNI CGCG 3 cut(s) 115, 410, 509
BstH2I RGCGCY 3 cut(s) 342, 428, 548
BstHHI GCGC 5 cut(s) 341, 427, 509, 547, 787
BstMAI GTCTC 3 cut(s) 54, 312, 424
BstMCI CGRYCG 1 cut(s) 438
BstMWI GCNNNNNNNGC 9 cut(s) 73, 76, 82, 117, 378, 413, 422, 553, 603
BstNI CCWGG 1 cut(s) 737
BstSCI CCNGG 3 cut(s) 526, 735, 862
BstSLI GKGCMC 1 cut(s) 113
BstUI CGCG 3 cut(s) 115, 410, 509
BstV1I GCAGC 3 cut(s) 384, 534, 739
BstV2I GAAGAC 3 cut(s) 423, 573, 609
BstX2I RGATCY 1 cut(s) 815
BstYI RGATCY 1 cut(s) 815
BsuRI GGCC 3 cut(s) 76, 191, 416
BtgI CCRYGG 2 cut(s) 113, 408
BtgZI GCGATG 1 cut(s) 279
BtrI CACGTC 2 cut(s) 615, 628
BtsCI GGATG 1 cut(s) 254
BtsIMutI CAGTG 3 cut(s) 24, 766, 807
BveI ACCTGC 1 cut(s) 778
Cac8I GCNNGC 4 cut(s) 99, 113, 193, 659
CfoI GCGC 5 cut(s) 341, 427, 509, 547, 787
Cfr10I RCCGGY 2 cut(s) 63, 437
Cfr13I GGNCC 2 cut(s) 207, 845
Cfr42I CCGCGG 2 cut(s) 116, 411
CseI GACGC 2 cut(s) 208, 814
Csp6I GTAC 1 cut(s) 561
CviQI GTAC 1 cut(s) 561
DdeI CTNAG 2 cut(s) 71, 421
EciI GGCGGA 1 cut(s) 309
Ecl136II GAGCTC 1 cut(s) 381
Eco147I AGGCCT 1 cut(s) 191
Eco24I GRGCYC 2 cut(s) 383, 528
Eco31I GGTCTC 1 cut(s) 54
Eco47I GGWCC 2 cut(s) 207, 845
Eco53kI GAGCTC 1 cut(s) 381
Eco88I CYCGRG 2 cut(s) 49, 382
EcoICRI GAGCTC 1 cut(s) 381
EcoRII CCWGG 1 cut(s) 735
EcoT38I GRGCYC 2 cut(s) 383, 528
Esp3I CGTCTC 2 cut(s) 312, 424
FaiI YATR 2 cut(s) 471, 916
FaqI GGGAC 1 cut(s) 218
FauI CCCGC 2 cut(s) 120, 182
FbaI TGATCA 5 cut(s) 609, 667, 874, 907, 940
FokI GGATG 1 cut(s) 261
FriOI GRGCYC 2 cut(s) 383, 528
GlaI GCGC 5 cut(s) 340, 426, 508, 546, 786
GsaI CCCAGC 1 cut(s) 172
GsuI CTGGAG 2 cut(s) 572, 940
HaeII RGCGCY 3 cut(s) 342, 428, 548
HaeIII GGCC 3 cut(s) 76, 191, 416
HapII CCGG 5 cut(s) 64, 438, 476, 528, 863
HgaI GACGC 2 cut(s) 208, 814
HhaI GCGC 5 cut(s) 341, 427, 509, 547, 787
Hin1I GRCGYC 2 cut(s) 219, 825
Hin6I GCGC 5 cut(s) 339, 425, 507, 545, 785
HinP1I GCGC 5 cut(s) 339, 425, 507, 545, 785
HinfI GANTC 3 cut(s) 122, 479, 922
HpaII CCGG 5 cut(s) 64, 438, 476, 528, 863
HphI GGTGA 1 cut(s) 382
Hpy188I TCNGA 4 cut(s) 348, 586, 780, 830
Hpy188III TCNNGA 5 cut(s) 51, 476, 601, 643, 944
Hpy99I CGWCG 2 cut(s) 629, 830
HpyCH4III ACNGT 3 cut(s) 515, 849, 904
HpyCH4IV ACGT 3 cut(s) 231, 614, 627
HpyCH4V TGCA 2 cut(s) 4, 751
HpyF10VI GCNNNNNNNGC 9 cut(s) 73, 76, 82, 117, 378, 413, 422, 553, 603
HpyF3I CTNAG 2 cut(s) 71, 421
HpySE526I ACGT 3 cut(s) 231, 614, 627
Hsp92I GRCGYC 2 cut(s) 219, 825
HspAI GCGC 5 cut(s) 339, 425, 507, 545, 785
Kpn2I TCCGGA 1 cut(s) 475
Ksp22I TGATCA 5 cut(s) 609, 667, 874, 907, 940
KspI CCGCGG 2 cut(s) 116, 411
LmnI GCTCC 3 cut(s) 272, 553, 594
Lsp1109I GCAGC 3 cut(s) 384, 534, 739
LweI GCATC 1 cut(s) 738
MaeII ACGT 3 cut(s) 231, 614, 627
MaeIII GTNAC 1 cut(s) 227
MboII GAAGA 8 cut(s) 31, 279, 423, 481, 573, 609, 757, 884
MflI RGATCY 1 cut(s) 815
MhlI GDGCHC 3 cut(s) 113, 383, 528
MluCI AATT 4 cut(s) 280, 451, 485, 839
MlyI GAGTC 1 cut(s) 931
MmeI TCCRAC 4 cut(s) 645, 808, 831, 843
MroI TCCGGA 1 cut(s) 475
MroXI GAANNNNTTC 1 cut(s) 580
MseI TTAA 1 cut(s) 570
MspA1I CMGCKG 2 cut(s) 115, 410
MspI CCGG 5 cut(s) 64, 438, 476, 528, 863
MspR9I CCNGG 3 cut(s) 528, 737, 864
MvaI CCWGG 1 cut(s) 737
MvnI CGCG 3 cut(s) 115, 410, 509
MwoI GCNNNNNNNGC 9 cut(s) 73, 76, 82, 117, 378, 413, 422, 553, 603
NciI CCSGG 2 cut(s) 528, 864
PaeR7I CTCGAG 1 cut(s) 382
PceI AGGCCT 1 cut(s) 191
PcsI WCGNNNNNNNCGW 2 cut(s) 296, 302
PdmI GAANNNNTTC 1 cut(s) 580
PfeI GAWTC 2 cut(s) 122, 479
PflFI GACNNNGTC 1 cut(s) 304
PleI GAGTC 1 cut(s) 930
PpsI GAGTC 1 cut(s) 930
Psp124BI GAGCTC 1 cut(s) 383
Psp6I CCWGG 1 cut(s) 735
PspFI CCCAGC 1 cut(s) 168
PspGI CCWGG 1 cut(s) 735
PspPI GGNCC 2 cut(s) 207, 845
PspXI VCTCGAGB 1 cut(s) 382
PsuI RGATCY 1 cut(s) 815
PsyI GACNNNGTC 1 cut(s) 304
RsaI GTAC 1 cut(s) 562
RsaNI GTAC 1 cut(s) 561
SacI GAGCTC 1 cut(s) 383
SacII CCGCGG 2 cut(s) 116, 411
SaqAI TTAA 1 cut(s) 570
Sau96I GGNCC 2 cut(s) 207, 845
SchI GAGTC 1 cut(s) 931
ScrFI CCNGG 3 cut(s) 528, 737, 864
SduI GDGCHC 3 cut(s) 113, 383, 528
SfaNI GCATC 1 cut(s) 738
Sfr274I CTCGAG 1 cut(s) 382
Sfr303I CCGCGG 2 cut(s) 116, 411
SgrBI CCGCGG 2 cut(s) 116, 411
SinI GGWCC 2 cut(s) 207, 845
SlaI CTCGAG 1 cut(s) 382
SmlI CTYRAG 1 cut(s) 382
SmoI CTYRAG 1 cut(s) 382
Sse9I AATT 4 cut(s) 280, 451, 485, 839
SseBI AGGCCT 1 cut(s) 191
SspI AATATT 1 cut(s) 466
SstI GAGCTC 1 cut(s) 383
StuI AGGCCT 1 cut(s) 191
StyD4I CCNGG 3 cut(s) 526, 735, 862
TaaI ACNGT 3 cut(s) 515, 849, 904
TaiI ACGT 3 cut(s) 234, 617, 630
TaqI TCGA 4 cut(s) 368, 383, 434, 792
TasI AATT 4 cut(s) 280, 451, 485, 839
TfiI GAWTC 2 cut(s) 122, 479
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TscAI CASTG 3 cut(s) 24, 766, 807
TseI GCWGC 3 cut(s) 372, 547, 727
TspDTI ATGAA 4 cut(s) 273, 458, 481, 698
TspGWI ACGGA 3 cut(s) 215, 578, 588
TspRI CASTG 3 cut(s) 24, 766, 807
Tth111I GACNNNGTC 1 cut(s) 304
VpaK11BI GGWCC 2 cut(s) 207, 845
XapI RAATTY 3 cut(s) 280, 485, 839
XhoI CTCGAG 1 cut(s) 382
XmnI GAANNNNTTC 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.