pycom15g00370

Belongs to the glycosyl hydrolase 13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006625
Physical Location & Seq
Reverse (-)
241575 .. 244705
3131 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1017 bp
ATGGATTACCTACGCAGCAATGATTCACGAGAAAATGCGCAGCAGACTGATATTGGTGCAGCAGTGCGCAATGGAAGAGAAATCCTTTTTCAGGCCTTCAATTGGGAGTCTCATAAACATGATTGGTGGAGAAATTTAGAAACAAAAGTTCCGGATATAGGAAGTTCTGGTTTTACGTCAGCATGGTTGCCACCAGCAACTCAATCCTTTGCACCAGAAGGTTACCTTCCGCAGGACATATATTCCCTCAATTCTAAATATGGATCTGAGAACTTATTAAAATCTTTACTTCAAAAAATGAAGCAACATAAAGTTAGAGCAATGGCTGACATAGTTATCAATCATCGTGTTGGGACTACCCGAGGGCATGGTGGAATGTACAACCGCTATGATGGAATTTCATTGTCATGGGATGAACATGCTGTCACATCTTGTACCGGTGGACTGGGTAATCAAAGCACTGGTGACAATTTCCATGGGGTTCCGAATATTGATCATAGCCAACTTTTTGTTCGAAAAGATATTACAGGATGGCTGCAGTGGCTACGTAATAATGTTGGTTTTCAGGATTTCCGATTTGATTTTGCAAGGGGTTATTCAGCAAAATATGTGAAAGAATACATTGAAGGAGCAAAACCCATTTTTTCTGTTGGGGAGTATTGGGATTCTTGCAACTACAACGATCATGGCCTGGATTACAACCAAGATAGCCACAGACAGCGGATAGTAAATTGGATTAATGGCACTGGACAGCTATCAACTGCGTTTGACTTCACAACCAAGGGAATTCTTCAGGAAGCTGTTAAAGGGCAATTGTGGCGCCTGCGTGACCCCCAAGGGAAGCCACCAGGTTTAATTGGATGGTGGCCTTCAAGAGCTGTCACATTCCTAGATAACCATGATACAGGCTCAACGCAGGCTCATTGGCCCTTCCCTTCAAATCATATTATGGAGGGTTACGCATACATACTCATGCACCCAGGAATACCAACAGTTTTCTACGATCGATTTCTATGA

Protein Analysis

339

Amino Acids

38.67

Weight (kDa)

6.7

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha-amylase PF00128 53 - 116 8.3e-09 Alpha amylase, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 39, 68
AccB1I GGYRCC 1 cut(s) 819
AccIII TCCGGA 1 cut(s) 151
AciI CCGC 3 cut(s) 230, 385, 721
AclWI GGATC 1 cut(s) 271
AcsI RAATTY 3 cut(s) 133, 396, 786
AcuI CTGAAG 1 cut(s) 776
AcyI GRCGYC 1 cut(s) 820
AfaI GTAC 2 cut(s) 380, 436
AfiI CCNNNNNNNGG 4 cut(s) 91, 102, 158, 232
AgeI ACCGGT 1 cut(s) 437
AgsI TTSAA 5 cut(s) 100, 293, 626, 873, 939
AjnI CCWGG 3 cut(s) 690, 847, 979
AjuI GAANNNNNNNTTGG 2 cut(s) 495, 527
AluBI AGCT 3 cut(s) 754, 800, 878
AluI AGCT 3 cut(s) 754, 800, 878
Alw26I GTCTC 1 cut(s) 114
AlwI GGATC 1 cut(s) 271
Ama87I CYCGRG 1 cut(s) 360
Aor13HI TCCGGA 1 cut(s) 151
AoxI GGCC 4 cut(s) 93, 688, 866, 926
ApeKI GCWGC 4 cut(s) 15, 40, 59, 535
ApoI RAATTY 3 cut(s) 133, 396, 786
AseI ATTAAT 1 cut(s) 738
AsiGI ACCGGT 1 cut(s) 437
AspLEI GCGC 3 cut(s) 40, 69, 822
AspS9I GGNCC 1 cut(s) 927
AsuHPI GGTGA 1 cut(s) 476
AsuII TTCGAA 1 cut(s) 514
AvaI CYCGRG 1 cut(s) 360
BanI GGYRCC 1 cut(s) 819
BauI CACGAG 1 cut(s) 27
BbvI GCAGC 4 cut(s) 27, 52, 71, 522
BccI CCATC 3 cut(s) 386, 525, 855
BciT130I CCWGG 3 cut(s) 692, 849, 981
BclI TGATCA 1 cut(s) 493
BcoDI GTCTC 1 cut(s) 114
BfaI CTAG 1 cut(s) 890
BfmI CTRYAG 1 cut(s) 536
BfoI RGCGCY 1 cut(s) 823
BisI GCNGC 4 cut(s) 16, 41, 60, 536
BlsI GCNGC 4 cut(s) 17, 42, 61, 537
Bme1390I CCNGG 3 cut(s) 692, 849, 981
BmeT110I CYCGRG 1 cut(s) 360
BmgT120I GGNCC 1 cut(s) 927
BmiI GGNNCC 2 cut(s) 483, 821
BmrFI CCNGG 3 cut(s) 692, 849, 981
BmrI ACTGGG 1 cut(s) 455
BmuI ACTGGG 1 cut(s) 455
Bpu14I TTCGAA 1 cut(s) 514
Bsa29I ATCGAT 1 cut(s) 1006
BsaAI YACGTR 1 cut(s) 548
BsaHI GRCGYC 1 cut(s) 820
BsaJI CCNNGG 5 cut(s) 361, 475, 780, 835, 979
BsaWI WCCGGW 2 cut(s) 151, 437
Bsc4I CCNNNNNNNGG 4 cut(s) 91, 102, 158, 232
Bse118I RCCGGY 1 cut(s) 437
Bse1I ACTGG 3 cut(s) 450, 466, 751
Bse3DI GCAATG 3 cut(s) 25, 76, 327
BseAI TCCGGA 1 cut(s) 151
BseBI CCWGG 3 cut(s) 692, 849, 981
BseCI ATCGAT 1 cut(s) 1006
BseDI CCNNGG 5 cut(s) 361, 475, 780, 835, 979
BseGI GGATG 3 cut(s) 418, 536, 866
BseLI CCNNNNNNNGG 4 cut(s) 91, 102, 158, 232
BseMI GCAATG 3 cut(s) 25, 76, 327
BseMII CTCAG 1 cut(s) 258
BseNI ACTGG 3 cut(s) 450, 466, 751
BseXI GCAGC 4 cut(s) 27, 52, 71, 522
BsgI GTGCAG 1 cut(s) 78
Bsh1285I CGRYCG 1 cut(s) 1006
BshFI GGCC 4 cut(s) 95, 690, 868, 928
BshNI GGYRCC 1 cut(s) 819
BshTI ACCGGT 1 cut(s) 437
BshVI ATCGAT 1 cut(s) 1006
BsiEI CGRYCG 1 cut(s) 1006
BsiHKCI CYCGRG 1 cut(s) 360
BsiSI CCGG 2 cut(s) 152, 438
BslFI GGGAC 1 cut(s) 367
BslI CCNNNNNNNGG 4 cut(s) 91, 102, 158, 232
BsmAI GTCTC 1 cut(s) 114
BsmFI GGGAC 1 cut(s) 367
BsnI GGCC 4 cut(s) 95, 690, 868, 928
BsoBI CYCGRG 1 cut(s) 360
Bsp119I TTCGAA 1 cut(s) 514
Bsp13I TCCGGA 1 cut(s) 151
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 4 cut(s) 263, 493, 682, 1003
Bsp19I CCATGG 1 cut(s) 475
BspACI CCGC 3 cut(s) 230, 385, 721
BspANI GGCC 4 cut(s) 95, 690, 868, 928
BspCNI CTCAG 1 cut(s) 259
BspDI ATCGAT 1 cut(s) 1006
BspEI TCCGGA 1 cut(s) 151
BspLI GGNNCC 2 cut(s) 483, 821
BspMAI CTGCAG 1 cut(s) 540
BspPI GGATC 1 cut(s) 271
BspT104I TTCGAA 1 cut(s) 514
BspT107I GGYRCC 1 cut(s) 819
BsrDI GCAATG 3 cut(s) 25, 76, 327
BsrFI RCCGGY 1 cut(s) 437
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 3 cut(s) 450, 466, 751
BssAI RCCGGY 1 cut(s) 437
BssECI CCNNGG 5 cut(s) 361, 475, 780, 835, 979
BssMI GATC 4 cut(s) 263, 493, 682, 1003
BssNI GRCGYC 1 cut(s) 820
BssSI CACGAG 1 cut(s) 27
BssT1I CCWWGG 3 cut(s) 475, 780, 835
Bst2BI CACGAG 1 cut(s) 27
Bst2UI CCWGG 3 cut(s) 692, 849, 981
Bst4CI ACNGT 1 cut(s) 994
Bst6I CTCTTC 1 cut(s) 70
BstACI GRCGYC 1 cut(s) 820
BstAUI TGTACA 1 cut(s) 378
BstBAI YACGTR 1 cut(s) 548
BstBI TTCGAA 1 cut(s) 514
BstC8I GCNNGC 2 cut(s) 824, 918
BstDEI CTNAG 1 cut(s) 267
BstDSI CCRYGG 1 cut(s) 475
BstEII GGTNACC 1 cut(s) 221
BstENI CCTNNNNNAGG 2 cut(s) 89, 230
BstF5I GGATG 3 cut(s) 418, 536, 866
BstH2I RGCGCY 1 cut(s) 823
BstHHI GCGC 3 cut(s) 40, 69, 822
BstKTI GATC 4 cut(s) 266, 496, 685, 1006
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 4 cut(s) 263, 493, 682, 1003
BstMCI CGRYCG 1 cut(s) 1006
BstMWI GCNNNNNNNGC 2 cut(s) 541, 817
BstNI CCWGG 3 cut(s) 692, 849, 981
BstNSI RCATGY 1 cut(s) 422
BstPI GGTNACC 1 cut(s) 221
BstSCI CCNGG 3 cut(s) 690, 847, 979
BstSFI CTRYAG 1 cut(s) 536
BstSNI TACGTA 1 cut(s) 548
BstV1I GCAGC 4 cut(s) 27, 52, 71, 522
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
Bsu15I ATCGAT 1 cut(s) 1006
BsuRI GGCC 4 cut(s) 95, 690, 868, 928
BsuTUI ATCGAT 1 cut(s) 1006
BtgI CCRYGG 1 cut(s) 475
BtsCI GGATG 3 cut(s) 418, 536, 866
BtsI GCAGTG 2 cut(s) 69, 545
BtsIMutI CAGTG 4 cut(s) 69, 459, 545, 744
Cac8I GCNNGC 2 cut(s) 824, 918
CfoI GCGC 3 cut(s) 40, 69, 822
Cfr10I RCCGGY 1 cut(s) 437
Cfr13I GGNCC 1 cut(s) 927
ClaI ATCGAT 1 cut(s) 1006
CsiI ACCWGGT 1 cut(s) 847
Csp6I GTAC 2 cut(s) 379, 435
CspAI ACCGGT 1 cut(s) 437
CviAII CATG 9 cut(s) 119, 183, 368, 408, 419, 476, 686, 899, 973
CviQI GTAC 2 cut(s) 379, 435
DdeI CTNAG 1 cut(s) 267
DinI GGCGCC 1 cut(s) 821
DpnI GATC 4 cut(s) 265, 495, 684, 1005
DpnII GATC 4 cut(s) 263, 493, 682, 1003
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Eco105I TACGTA 1 cut(s) 548
Eco130I CCWWGG 3 cut(s) 475, 780, 835
Eco147I AGGCCT 1 cut(s) 95
Eco57I CTGAAG 1 cut(s) 776
Eco88I CYCGRG 1 cut(s) 360
Eco91I GGTNACC 1 cut(s) 221
EcoNI CCTNNNNNAGG 2 cut(s) 89, 230
EcoO65I GGTNACC 1 cut(s) 221
EcoRI GAATTC 1 cut(s) 786
EcoRII CCWGG 3 cut(s) 690, 847, 979
EcoT14I CCWWGG 3 cut(s) 475, 780, 835
EgeI GGCGCC 1 cut(s) 821
EheI GGCGCC 1 cut(s) 821
ErhI CCWWGG 3 cut(s) 475, 780, 835
FaeI CATG 9 cut(s) 122, 186, 371, 411, 422, 479, 689, 902, 976
FalI AAGNNNNNCTT 2 cut(s) 210, 242
FaqI GGGAC 1 cut(s) 367
FatI CATG 9 cut(s) 118, 182, 367, 407, 418, 475, 685, 898, 972
FbaI TGATCA 1 cut(s) 493
Fnu4HI GCNGC 4 cut(s) 16, 41, 60, 536
FokI GGATG 3 cut(s) 425, 543, 873
Fsp4HI GCNGC 4 cut(s) 16, 41, 60, 536
FspBI CTAG 1 cut(s) 890
FspI TGCGCA 2 cut(s) 39, 68
GlaI GCGC 3 cut(s) 39, 68, 821
GluI GCNGC 4 cut(s) 16, 41, 60, 536
HaeII RGCGCY 1 cut(s) 823
HaeIII GGCC 4 cut(s) 95, 690, 868, 928
HapII CCGG 2 cut(s) 152, 438
HhaI GCGC 3 cut(s) 40, 69, 822
Hin1I GRCGYC 1 cut(s) 820
Hin1II CATG 9 cut(s) 122, 186, 371, 411, 422, 479, 689, 902, 976
Hin6I GCGC 3 cut(s) 38, 67, 820
HinP1I GCGC 3 cut(s) 38, 67, 820
HinfI GANTC 3 cut(s) 23, 107, 665
HpaII CCGG 2 cut(s) 152, 438
HphI GGTGA 1 cut(s) 476
Hpy166II GTNNAC 1 cut(s) 443
Hpy188I TCNGA 3 cut(s) 268, 486, 575
Hpy188III TCNNGA 5 cut(s) 27, 152, 566, 794, 873
Hpy8I GTNNAC 1 cut(s) 443
HpyAV CCTTC 7 cut(s) 106, 212, 236, 620, 879, 940, 945
HpyCH4III ACNGT 1 cut(s) 994
HpyCH4IV ACGT 2 cut(s) 176, 547
HpyCH4V TGCA 6 cut(s) 59, 212, 538, 587, 672, 976
HpyF10VI GCNNNNNNNGC 2 cut(s) 541, 817
HpyF3I CTNAG 1 cut(s) 267
HpySE526I ACGT 2 cut(s) 176, 547
Hsp92I GRCGYC 1 cut(s) 820
Hsp92II CATG 9 cut(s) 122, 186, 371, 411, 422, 479, 689, 902, 976
HspAI GCGC 3 cut(s) 38, 67, 820
KasI GGCGCC 1 cut(s) 819
Kpn2I TCCGGA 1 cut(s) 151
Ksp22I TGATCA 1 cut(s) 493
Kzo9I GATC 4 cut(s) 263, 493, 682, 1003
LmnI GCTCC 1 cut(s) 629
Lsp1109I GCAGC 4 cut(s) 27, 52, 71, 522
MabI ACCWGGT 1 cut(s) 847
MaeI CTAG 1 cut(s) 890
MaeII ACGT 2 cut(s) 176, 547
MaeIII GTNAC 6 cut(s) 221, 424, 464, 827, 880, 956
MalI GATC 4 cut(s) 265, 495, 684, 1005
MboI GATC 4 cut(s) 263, 493, 682, 1003
MboII GAAGA 2 cut(s) 87, 782
MfeI CAATTG 2 cut(s) 100, 812
MflI RGATCY 1 cut(s) 263
MluCI AATT 9 cut(s) 100, 133, 250, 396, 469, 730, 786, 812, 855
Mly113I GGCGCC 1 cut(s) 820
MlyI GAGTC 1 cut(s) 116
MnlI CCTC 3 cut(s) 257, 356, 946
MroI TCCGGA 1 cut(s) 151
MseI TTAA 4 cut(s) 278, 738, 804, 854
MslI CAYNNNNRTG 3 cut(s) 117, 406, 971
MspA1I CMGCKG 1 cut(s) 721
MspI CCGG 2 cut(s) 152, 438
MspR9I CCNGG 3 cut(s) 692, 849, 981
MunI CAATTG 2 cut(s) 100, 812
MvaI CCWGG 3 cut(s) 692, 849, 981
MwoI GCNNNNNNNGC 2 cut(s) 541, 817
NarI GGCGCC 1 cut(s) 820
NcoI CCATGG 1 cut(s) 475
NdeII GATC 4 cut(s) 263, 493, 682, 1003
NlaIII CATG 9 cut(s) 122, 186, 371, 411, 422, 479, 689, 902, 976
NlaIV GGNNCC 2 cut(s) 483, 821
NmuCI GTSAC 4 cut(s) 424, 464, 827, 880
NsbI TGCGCA 2 cut(s) 39, 68
NspI RCATGY 1 cut(s) 422
NspV TTCGAA 1 cut(s) 514
PceI AGGCCT 1 cut(s) 95
PfeI GAWTC 2 cut(s) 23, 665
PinAI ACCGGT 1 cut(s) 437
PkrI GCNGC 4 cut(s) 17, 42, 61, 537
Ple19I CGATCG 1 cut(s) 1006
PleI GAGTC 1 cut(s) 115
PluTI GGCGCC 1 cut(s) 823
PpsI GAGTC 1 cut(s) 115
Ppu21I YACGTR 1 cut(s) 548
PshBI ATTAAT 1 cut(s) 738
Psp6I CCWGG 3 cut(s) 690, 847, 979
PspEI GGTNACC 1 cut(s) 221
PspGI CCWGG 3 cut(s) 690, 847, 979
PspN4I GGNNCC 2 cut(s) 483, 821
PspPI GGNCC 1 cut(s) 927
PstI CTGCAG 1 cut(s) 540
PsuI RGATCY 1 cut(s) 263
PvuI CGATCG 1 cut(s) 1006
RsaI GTAC 2 cut(s) 380, 436
RsaNI GTAC 2 cut(s) 379, 435
RseI CAYNNNNRTG 3 cut(s) 117, 406, 971
SaqAI TTAA 4 cut(s) 278, 738, 804, 854
SatI GCNGC 4 cut(s) 16, 41, 60, 536
Sau3AI GATC 4 cut(s) 263, 493, 682, 1003
Sau96I GGNCC 1 cut(s) 927
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 3 cut(s) 692, 849, 981
SetI ASST 9 cut(s) 12, 179, 223, 228, 550, 756, 802, 853, 880
SexAI ACCWGGT 1 cut(s) 847
SfcI CTRYAG 1 cut(s) 536
SfoI GGCGCC 1 cut(s) 821
SfuI TTCGAA 1 cut(s) 514
SmiMI CAYNNNNRTG 3 cut(s) 117, 406, 971
SnaBI TACGTA 1 cut(s) 548
Sse9I AATT 9 cut(s) 100, 133, 250, 396, 469, 730, 786, 812, 855
SseBI AGGCCT 1 cut(s) 95
SsiI CCGC 3 cut(s) 230, 385, 721
SspDI GGCGCC 1 cut(s) 819
SspI AATATT 1 cut(s) 490
SspMI CTAG 1 cut(s) 890
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 3 cut(s) 690, 847, 979
StyI CCWWGG 3 cut(s) 475, 780, 835
TaaI ACNGT 1 cut(s) 994
TaiI ACGT 2 cut(s) 179, 550
TaqI TCGA 2 cut(s) 514, 1006
TasI AATT 9 cut(s) 100, 133, 250, 396, 469, 730, 786, 812, 855
TatI WGTACW 1 cut(s) 378
TfiI GAWTC 2 cut(s) 23, 665
Tru1I TTAA 4 cut(s) 278, 738, 804, 854
Tru9I TTAA 4 cut(s) 278, 738, 804, 854
TscAI CASTG 4 cut(s) 69, 466, 545, 751
TseFI GTSAC 4 cut(s) 424, 464, 827, 880
TseI GCWGC 4 cut(s) 15, 40, 59, 535
Tsp45I GTSAC 4 cut(s) 424, 464, 827, 880
TspDTI ATGAA 3 cut(s) 314, 390, 429
TspRI CASTG 4 cut(s) 69, 466, 545, 751
VspI ATTAAT 1 cut(s) 738
XagI CCTNNNNNAGG 2 cut(s) 89, 230
XapI RAATTY 3 cut(s) 133, 396, 786
XceI RCATGY 1 cut(s) 422
XspI CTAG 1 cut(s) 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.