pycom15g02850

1-acyl-sn-glycerol-3-phosphate acyltransferase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008281
Physical Location & Seq
Reverse (-)
1761785 .. 1763021
1237 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 744 bp
ATGAAGATACCTGTTTTCGGTTGGGGGTTTCACATTCTAGAGTTCATCCCGTTGAAGAGGAAGTGGGAAGCTGATGAACCGGTTATGCGGAAAATGCTTTCATCATTTGCTGATCCTGCAGACCCTCTTTGGCTCGCCATTTTTCCTGAAGGAACTGATTATAATGAAGAAAAATGCAAAAAGAGTCAGTTGTTTGCTTCTGAAACTGGACTTCCTGTACTGTCATATGTGCTGCTTCCAAGAACAAAAGGCTTTTGTGCTTGCTTGGAAACTCTAAGAAGTTCTTTGGATGCAGTTTATGACTTGACGATCGCATACAAGAATCAATGCCCTTCGTTTCTAGACAATGCGTTCGGCGTGGATCCATCAGAAGTTCACATACATGTTCGGCGCATCCCTATCGAAGAGATCCCCGTTTCTAACACCGATGCAGCCTCTTGGTTAACAGAAGCATTCAGGCTCAAGGACAATCTACTGTCCGATTTTGACGATCAAGGCCATTTTCCTAATGAAGGAGGAGAAGAAGAGCTTTCTACATTCAAGTGCTTGGTAAATTTTATCTTGGTAATTGTTTTGACTATCATGCTGATTTACCTAGCCATTTTTTCATCTGTCTGGTTTAAAGTATACATCGGTTTATCATGTGCTTACCTTGCCACAGCCACTTATTTTGATTTTCAACCGATGCCAATTTTAGACTTTGTTCAAGCAACATGTGTTTGCAATAGAGCAAGAATTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110
Pfam Domains
Protein Families
Acyltransf_C Acyltransferase

Protein Analysis

248

Amino Acids

28.09

Weight (kDa)

4.85

Isoelectric Point (pI)

50.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyltransf_C PF16076 119 - 188 8.7e-17 Acyltransferase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 162
AccI GTMKAC 1 cut(s) 627
AciI CCGC 1 cut(s) 88
AclWI GGATC 4 cut(s) 107, 356, 369, 403
AcsI RAATTY 1 cut(s) 553
AcuI CTGAAG 1 cut(s) 168
AfaI GTAC 1 cut(s) 219
AfiI CCNNNNNNNGG 2 cut(s) 17, 512
AflIII ACRYGT 2 cut(s) 382, 713
AgeI ACCGGT 1 cut(s) 79
AgsI TTSAA 5 cut(s) 55, 541, 680, 707, 740
AluBI AGCT 2 cut(s) 71, 529
AluI AGCT 2 cut(s) 71, 529
AlwI GGATC 4 cut(s) 107, 356, 369, 403
AoxI GGCC 1 cut(s) 496
ApeKI GCWGC 2 cut(s) 232, 431
ApoI RAATTY 1 cut(s) 553
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
AsiGI ACCGGT 1 cut(s) 79
AspLEI GCGC 1 cut(s) 393
BamHI GGATCC 1 cut(s) 361
BarI GAAGNNNNNNTAC 2 cut(s) 363, 395
BbvI GCAGC 2 cut(s) 219, 443
BccI CCATC 1 cut(s) 373
BcgI CGANNNNNNTGC 2 cut(s) 382, 416
BfaI CTAG 3 cut(s) 38, 341, 596
BfmI CTRYAG 1 cut(s) 117
BisI GCNGC 2 cut(s) 233, 432
BlsI GCNGC 2 cut(s) 234, 433
BmiI GGNNCC 1 cut(s) 363
BmsI GCATC 4 cut(s) 280, 402, 418, 675
BpuEI CTTGAG 1 cut(s) 446
BsaWI WCCGGW 1 cut(s) 79
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 512
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 1 cut(s) 211
BseGI GGATG 3 cut(s) 45, 295, 393
BseLI CCNNNNNNNGG 2 cut(s) 17, 512
BseNI ACTGG 1 cut(s) 211
BseRI GAGGAG 1 cut(s) 531
BseXI GCAGC 2 cut(s) 219, 443
Bsh1285I CGRYCG 1 cut(s) 312
BshFI GGCC 1 cut(s) 498
BshTI ACCGGT 1 cut(s) 79
BsiEI CGRYCG 1 cut(s) 312
BsiSI CCGG 1 cut(s) 80
BslI CCNNNNNNNGG 2 cut(s) 17, 512
BsmI GAATGC 1 cut(s) 452
BsnI GGCC 1 cut(s) 498
Bsp143I GATC 5 cut(s) 112, 309, 361, 408, 490
BspACI CCGC 1 cut(s) 88
BspANI GGCC 1 cut(s) 498
BspLI GGNNCC 1 cut(s) 363
BspMAI CTGCAG 1 cut(s) 121
BspPI GGATC 4 cut(s) 107, 356, 369, 403
BspQI GCTCTTC 1 cut(s) 519
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 1 cut(s) 211
BssAI RCCGGY 1 cut(s) 79
BssMI GATC 5 cut(s) 112, 309, 361, 408, 490
BssNAI GTATAC 1 cut(s) 628
Bst1107I GTATAC 1 cut(s) 628
Bst4CI ACNGT 2 cut(s) 222, 477
Bst6I CTCTTC 3 cut(s) 50, 399, 519
BstC8I GCNNGC 2 cut(s) 135, 262
BstDEI CTNAG 1 cut(s) 275
BstENI CCTNNNNNAGG 1 cut(s) 510
BstF5I GGATG 3 cut(s) 45, 295, 393
BstHHI GCGC 1 cut(s) 393
BstKTI GATC 5 cut(s) 115, 312, 364, 411, 493
BstMBI GATC 5 cut(s) 112, 309, 361, 408, 490
BstMCI CGRYCG 1 cut(s) 312
BstMWI GCNNNNNNNGC 3 cut(s) 94, 116, 653
BstNSI RCATGY 2 cut(s) 386, 717
BstSFI CTRYAG 1 cut(s) 117
BstV1I GCAGC 2 cut(s) 219, 443
BstX2I RGATCY 2 cut(s) 361, 408
BstYI RGATCY 2 cut(s) 361, 408
BstZ17I GTATAC 1 cut(s) 628
BsuRI GGCC 1 cut(s) 498
BtsCI GGATG 3 cut(s) 45, 295, 393
Cac8I GCNNGC 2 cut(s) 135, 262
CfoI GCGC 1 cut(s) 393
Cfr10I RCCGGY 1 cut(s) 79
Csp6I GTAC 1 cut(s) 218
CspAI ACCGGT 1 cut(s) 79
CspCI CAANNNNNGTGG 2 cut(s) 652, 687
CviAII CATG 4 cut(s) 383, 583, 642, 714
CviJI RGCY 9 cut(s) 71, 133, 252, 434, 460, 498, 529, 599, 662
CviKI_1 RGCY 9 cut(s) 71, 133, 252, 434, 460, 498, 529, 599, 662
CviQI GTAC 1 cut(s) 218
DdeI CTNAG 1 cut(s) 275
DpnI GATC 5 cut(s) 114, 311, 363, 410, 492
DpnII GATC 5 cut(s) 112, 309, 361, 408, 490
DraI TTTAAA 1 cut(s) 622
Eam1104I CTCTTC 3 cut(s) 50, 399, 519
EarI CTCTTC 3 cut(s) 50, 399, 519
Eco57I CTGAAG 1 cut(s) 168
EcoNI CCTNNNNNAGG 1 cut(s) 510
FaeI CATG 4 cut(s) 386, 586, 645, 717
FalI AAGNNNNNCTT 4 cut(s) 268, 300, 513, 545
FatI CATG 4 cut(s) 382, 582, 641, 713
FauNDI CATATG 1 cut(s) 226
FblI GTMKAC 1 cut(s) 627
Fnu4HI GCNGC 2 cut(s) 233, 432
FokI GGATG 3 cut(s) 32, 302, 380
Fsp4HI GCNGC 2 cut(s) 233, 432
FspBI CTAG 3 cut(s) 38, 341, 596
GlaI GCGC 1 cut(s) 392
GluI GCNGC 2 cut(s) 233, 432
HaeIII GGCC 1 cut(s) 498
HapII CCGG 1 cut(s) 80
HhaI GCGC 1 cut(s) 393
Hin1II CATG 4 cut(s) 386, 586, 645, 717
Hin6I GCGC 1 cut(s) 391
HinP1I GCGC 1 cut(s) 391
HincII GTYRAC 1 cut(s) 444
HindII GTYRAC 1 cut(s) 444
HinfI GANTC 2 cut(s) 184, 322
HpaI GTTAAC 1 cut(s) 444
HpaII CCGG 1 cut(s) 80
Hpy166II GTNNAC 3 cut(s) 376, 444, 628
Hpy188I TCNGA 3 cut(s) 202, 370, 481
Hpy188III TCNNGA 3 cut(s) 38, 146, 341
Hpy8I GTNNAC 3 cut(s) 376, 444, 628
HpyAV CCTTC 3 cut(s) 143, 342, 506
HpyCH4III ACNGT 2 cut(s) 222, 477
HpyCH4V TGCA 5 cut(s) 119, 177, 293, 431, 723
HpyF10VI GCNNNNNNNGC 3 cut(s) 94, 116, 653
HpyF3I CTNAG 1 cut(s) 275
Hsp92II CATG 4 cut(s) 386, 586, 645, 717
HspAI GCGC 1 cut(s) 391
KspAI GTTAAC 1 cut(s) 444
Kzo9I GATC 5 cut(s) 112, 309, 361, 408, 490
LguI GCTCTTC 1 cut(s) 519
LpnPI CCDG 8 cut(s) 24, 93, 129, 159, 192, 228, 442, 601
Lsp1109I GCAGC 2 cut(s) 219, 443
LweI GCATC 4 cut(s) 280, 402, 418, 675
MaeI CTAG 3 cut(s) 38, 341, 596
MalI GATC 5 cut(s) 114, 311, 363, 410, 492
MboI GATC 5 cut(s) 112, 309, 361, 408, 490
MboII GAAGA 6 cut(s) 16, 67, 179, 416, 533, 536
MflI RGATCY 2 cut(s) 361, 408
MluCI AATT 4 cut(s) 553, 567, 690, 735
MlyI GAGTC 1 cut(s) 193
MnlI CCTC 4 cut(s) 51, 135, 445, 509
MseI TTAA 2 cut(s) 443, 621
MslI CAYNNNNRTG 2 cut(s) 381, 541
MspI CCGG 1 cut(s) 80
Mva1269I GAATGC 1 cut(s) 452
MwoI GCNNNNNNNGC 3 cut(s) 94, 116, 653
NdeI CATATG 1 cut(s) 226
NdeII GATC 5 cut(s) 112, 309, 361, 408, 490
NlaIII CATG 4 cut(s) 386, 586, 645, 717
NlaIV GGNNCC 1 cut(s) 363
NspI RCATGY 2 cut(s) 386, 717
PciI ACATGT 2 cut(s) 382, 713
PciSI GCTCTTC 1 cut(s) 519
PctI GAATGC 1 cut(s) 452
PfeI GAWTC 1 cut(s) 322
PinAI ACCGGT 1 cut(s) 79
PkrI GCNGC 2 cut(s) 234, 433
Ple19I CGATCG 1 cut(s) 312
PleI GAGTC 1 cut(s) 192
PpsI GAGTC 1 cut(s) 192
PscI ACATGT 2 cut(s) 382, 713
PsiI TTATAA 1 cut(s) 162
PspN4I GGNNCC 1 cut(s) 363
PstI CTGCAG 1 cut(s) 121
PsuI RGATCY 2 cut(s) 361, 408
PvuI CGATCG 1 cut(s) 312
RsaI GTAC 1 cut(s) 219
RsaNI GTAC 1 cut(s) 218
RseI CAYNNNNRTG 2 cut(s) 381, 541
SapI GCTCTTC 1 cut(s) 519
SaqAI TTAA 2 cut(s) 443, 621
SatI GCNGC 2 cut(s) 233, 432
Sau3AI GATC 5 cut(s) 112, 309, 361, 408, 490
SchI GAGTC 1 cut(s) 193
SetI ASST 5 cut(s) 13, 73, 531, 597, 654
SfaNI GCATC 4 cut(s) 280, 402, 418, 675
SfcI CTRYAG 1 cut(s) 117
SmiMI CAYNNNNRTG 2 cut(s) 381, 541
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
Sse9I AATT 4 cut(s) 553, 567, 690, 735
SsiI CCGC 1 cut(s) 88
SspMI CTAG 3 cut(s) 38, 341, 596
TaaI ACNGT 2 cut(s) 222, 477
TaqI TCGA 1 cut(s) 402
TasI AATT 4 cut(s) 553, 567, 690, 735
TatI WGTACW 1 cut(s) 217
TfiI GAWTC 1 cut(s) 322
Tru1I TTAA 2 cut(s) 443, 621
Tru9I TTAA 2 cut(s) 443, 621
TseI GCWGC 2 cut(s) 232, 431
TspDTI ATGAA 7 cut(s) 17, 34, 90, 90, 180, 525, 597
XagI CCTNNNNNAGG 1 cut(s) 510
XapI RAATTY 1 cut(s) 553
XbaI TCTAGA 2 cut(s) 37, 340
XceI RCATGY 2 cut(s) 386, 717
XmiI GTMKAC 1 cut(s) 627
XspI CTAG 3 cut(s) 38, 341, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.