RLG00000011338

1-acyl-sn-glycerol-3-phosphate acyltransferase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008281
Physical Location & Seq
Reverse (-)
8686745 .. 8688539
1795 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011338

Sequence Viewer

Length: 1191 bp
ATGAACTGCAGCTCCATTGAGGACGGTTCGTGGAAGGAGGTGGACGTTTGCAGTCCCTTAAAATCTGATAGTAAACTGAAGCACAGGCCCCTGAACGCTTTTAGAGTTGTAAGGGGTGTTCTATGTTTGGTGGTGTTTCTTTCCACTGCTTTCACCATTCTAGTCTGTTTTGCGCCGGTGGTAGCTGTTTTGTTGCGACTTTTTAGCATACATTACAGCAGAAAAGCAACATCCTTTCTATTTGCTAGGTGGCTGGCTCTCTGGCCCTTTCTATTTGAAAAGATCAACGGGACGAACGTGGTTTTTTCGGGCGATACTGTCCCACCTAGAGAGCGCATTTTGATTATTGCGAATCATAAAACTGAGGTGGATTGGATGTACTTGTGGGATCTTGCATTGCGAAAAGGGTCCCTCGGATATGTTAAATATGTGCTTAAGAGCAGCTTGATGAAGCTGCCTGTTTTTGGTTGGGGGTTCCACGTTCTAGAGTTCATTCCTTTGAAGAGGAAGTGGGAAGTTGATGAACCAATTCTCCGGAAAATGCTATTAAGTTTTGCTGATCCTCGGGACCCTCTCTGGCTTTCTATTTTTCCTGAAGGAACTGATTACGATGAAGAAAAATGCAAAAAGAGCCAGACATTTGCTGCTGAAAATGGACTTCCTGTGCTGTCACATGTGCTACTTCCAAGGACTAAAGGTTTTTGTGCTTGCTTAGAAGCCCTAAGAGGTTCTTTGGATGCAGTTTATGACTTGACAATTGCATACAAGAATCAATGCCCTTCGTTTATGGACAATGCATTTGGTGTAGATCCTTCAGAAGTACACATTCATGTTCGGCGTATCCCAATGGAAGATATCCCGGAGTCTAGCACAGATGCTGCATCTTGGTTAGTGGACACATTCCATCTCAAGGACAACATGCTGTCAGATTTCCATACTCGAGGCCATTTTCCTAATGAAGGAGGAGAAGAAGACATTTCTGCGTTCAAGGGTTTGGTAAATTTTATGTTGATACTTATTTTGACAGTGGTTATCATTTACCTTACCATTTTTTCAACGGTCTGGTTTAAAATATACATCGGTTTATCATGTGCATACCTTGCTTCCGCAACATATTTTGAAATCCAACCGGTGCCAGTATTAGACTTGGTTAAAGCAACATTGGTTTGCAATAGACTCAGATGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110
Pfam Domains
Protein Families
Acyltransf_C Acyltransferase

Protein Analysis

397

Amino Acids

45.08

Weight (kDa)

6.79

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyltransferase PF01553 101 - 234 2.6e-16 Acyltransferase
Acyltransf_C PF16076 268 - 336 5.3e-18 Acyltransferase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1132
AccIII TCCGGA 1 cut(s) 534
AciI CCGC 1 cut(s) 1107
AclWI GGATC 3 cut(s) 396, 554, 803
AcsI RAATTY 1 cut(s) 1000
AcuI CTGAAG 3 cut(s) 98, 615, 798
AfaI GTAC 2 cut(s) 380, 822
AfiI CCNNNNNNNGG 3 cut(s) 464, 910, 959
AflII CTTAAG 1 cut(s) 434
AflIII ACRYGT 1 cut(s) 673
AgeI ACCGGT 1 cut(s) 1129
AgsI TTSAA 5 cut(s) 278, 502, 988, 1056, 1121
AloI GAACNNNNNNTCC 3 cut(s) 28, 516, 548
AluBI AGCT 4 cut(s) 12, 185, 444, 454
AluI AGCT 4 cut(s) 12, 185, 444, 454
AlwI GGATC 3 cut(s) 396, 554, 803
AlwNI CAGNNNCTG 1 cut(s) 878
Ama87I CYCGRG 2 cut(s) 564, 939
Aor13HI TCCGGA 1 cut(s) 534
AoxI GGCC 3 cut(s) 86, 263, 943
ApeKI GCWGC 5 cut(s) 9, 441, 454, 644, 878
ApoI RAATTY 1 cut(s) 1000
ArsI GACNNNNNNTTYG 2 cut(s) 782, 814
AsiGI ACCGGT 1 cut(s) 1129
Asp700I GAANNNNTTC 1 cut(s) 528
AspLEI GCGC 2 cut(s) 175, 336
AspS9I GGNCC 4 cut(s) 87, 264, 408, 568
AsuC2I CCSGG 1 cut(s) 860
AsuHPI GGTGA 1 cut(s) 145
AvaI CYCGRG 2 cut(s) 564, 939
AvaII GGWCC 2 cut(s) 408, 568
BanI GGYRCC 1 cut(s) 1132
BbsI GAAGAC 1 cut(s) 978
BbvI GCAGC 5 cut(s) 21, 441, 453, 631, 865
BccI CCATC 1 cut(s) 912
BciVI GTATCC 1 cut(s) 851
BcnI CCSGG 1 cut(s) 860
BfaI CTAG 5 cut(s) 161, 246, 327, 485, 867
BfmI CTRYAG 1 cut(s) 7
BfrI CTTAAG 1 cut(s) 434
BfuI GTATCC 1 cut(s) 851
BisI GCNGC 5 cut(s) 10, 442, 455, 645, 879
BlsI GCNGC 5 cut(s) 11, 443, 456, 646, 880
Bme1390I CCNGG 1 cut(s) 860
Bme18I GGWCC 2 cut(s) 408, 568
BmeT110I CYCGRG 2 cut(s) 564, 939
BmgT120I GGNCC 4 cut(s) 87, 264, 408, 568
BmiI GGNNCC 7 cut(s) 89, 409, 410, 476, 569, 570, 1134
BmrFI CCNGG 1 cut(s) 860
BmsI GCATC 3 cut(s) 727, 865, 890
BpiI GAAGAC 1 cut(s) 978
BpuEI CTTGAG 1 cut(s) 893
BpuMI CCSGG 1 cut(s) 860
BsaJI CCNNGG 3 cut(s) 412, 563, 686
BsaWI WCCGGW 2 cut(s) 534, 1129
BsaXI ACNNNNNCTCC 3 cut(s) 26, 516, 546
Bsc4I CCNNNNNNNGG 3 cut(s) 464, 910, 959
Bse118I RCCGGY 2 cut(s) 175, 1129
Bse1I ACTGG 1 cut(s) 1136
Bse3DI GCAATG 1 cut(s) 395
BseAI TCCGGA 1 cut(s) 534
BseDI CCNNGG 3 cut(s) 412, 563, 686
BseGI GGATG 3 cut(s) 230, 381, 742
BseLI CCNNNNNNNGG 3 cut(s) 464, 910, 959
BseMI GCAATG 1 cut(s) 395
BseMII CTCAG 1 cut(s) 354
BseNI ACTGG 1 cut(s) 1136
BseRI GAGGAG 1 cut(s) 978
BseXI GCAGC 5 cut(s) 21, 441, 453, 631, 865
BshFI GGCC 3 cut(s) 88, 265, 945
BshNI GGYRCC 1 cut(s) 1132
BshTI ACCGGT 1 cut(s) 1129
BsiHKCI CYCGRG 2 cut(s) 564, 939
BsiSI CCGG 4 cut(s) 176, 535, 860, 1130
BslFI GGGAC 5 cut(s) 39, 304, 305, 394, 581
BslI CCNNNNNNNGG 3 cut(s) 464, 910, 959
BsmFI GGGAC 5 cut(s) 39, 304, 305, 394, 581
BsnI GGCC 3 cut(s) 88, 265, 945
BsoBI CYCGRG 2 cut(s) 564, 939
Bsp13I TCCGGA 1 cut(s) 534
Bsp143I GATC 4 cut(s) 282, 388, 559, 808
BspACI CCGC 1 cut(s) 1107
BspANI GGCC 3 cut(s) 88, 265, 945
BspCNI CTCAG 2 cut(s) 355, 1191
BspEI TCCGGA 1 cut(s) 534
BspLI GGNNCC 7 cut(s) 89, 409, 410, 476, 569, 570, 1134
BspMAI CTGCAG 1 cut(s) 11
BspPI GGATC 3 cut(s) 396, 554, 803
BspT107I GGYRCC 1 cut(s) 1132
BspTI CTTAAG 1 cut(s) 434
BsrDI GCAATG 1 cut(s) 395
BsrFI RCCGGY 2 cut(s) 175, 1129
BsrI ACTGG 1 cut(s) 1136
BssAI RCCGGY 2 cut(s) 175, 1129
BssECI CCNNGG 3 cut(s) 412, 563, 686
BssMI GATC 4 cut(s) 282, 388, 559, 808
BssT1I CCWWGG 1 cut(s) 686
Bst4CI ACNGT 4 cut(s) 26, 319, 1027, 1060
Bst6I CTCTTC 1 cut(s) 497
BstAFI CTTAAG 1 cut(s) 434
BstAPI GCANNNNNTGC 1 cut(s) 1100
BstC8I GCNNGC 2 cut(s) 255, 709
BstDEI CTNAG 4 cut(s) 363, 712, 722, 1178
BstENI CCTNNNNNAGG 1 cut(s) 957
BstF5I GGATG 3 cut(s) 230, 381, 742
BstHHI GCGC 2 cut(s) 175, 336
BstKTI GATC 4 cut(s) 285, 391, 562, 811
BstMBI GATC 4 cut(s) 282, 388, 559, 808
BstMWI GCNNNNNNNGC 2 cut(s) 630, 1100
BstNSI RCATGY 2 cut(s) 677, 922
BstSCI CCNGG 1 cut(s) 858
BstSFI CTRYAG 1 cut(s) 7
BstV1I GCAGC 5 cut(s) 21, 441, 453, 631, 865
BstV2I GAAGAC 1 cut(s) 978
BstX2I RGATCY 2 cut(s) 388, 808
BstYI RGATCY 2 cut(s) 388, 808
BsuI GTATCC 1 cut(s) 851
BsuRI GGCC 3 cut(s) 88, 265, 945
BtsCI GGATG 3 cut(s) 230, 381, 742
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 2 cut(s) 144, 1032
Cac8I GCNNGC 2 cut(s) 255, 709
CaiI CAGNNNCTG 1 cut(s) 878
CfoI GCGC 2 cut(s) 175, 336
Cfr10I RCCGGY 2 cut(s) 175, 1129
Cfr13I GGNCC 4 cut(s) 87, 264, 408, 568
Csp6I GTAC 2 cut(s) 379, 821
CspAI ACCGGT 1 cut(s) 1129
CviAII CATG 4 cut(s) 674, 830, 919, 1089
CviQI GTAC 2 cut(s) 379, 821
DdeI CTNAG 4 cut(s) 363, 712, 722, 1178
DpnI GATC 4 cut(s) 284, 390, 561, 810
DpnII GATC 4 cut(s) 282, 388, 559, 808
DraI TTTAAA 1 cut(s) 1069
Eam1104I CTCTTC 1 cut(s) 497
EarI CTCTTC 1 cut(s) 497
Eco130I CCWWGG 1 cut(s) 686
Eco32I GATATC 1 cut(s) 856
Eco47I GGWCC 2 cut(s) 408, 568
Eco57I CTGAAG 3 cut(s) 98, 615, 798
Eco88I CYCGRG 2 cut(s) 564, 939
EcoNI CCTNNNNNAGG 1 cut(s) 957
EcoO109I RGGNCCY 3 cut(s) 87, 408, 568
EcoRV GATATC 1 cut(s) 856
EcoT14I CCWWGG 1 cut(s) 686
EcoT22I ATGCAT 1 cut(s) 799
ErhI CCWWGG 1 cut(s) 686
FaeI CATG 4 cut(s) 677, 833, 922, 1092
FalI AAGNNNNNCTT 4 cut(s) 428, 460, 715, 747
FaqI GGGAC 5 cut(s) 39, 304, 305, 394, 581
FatI CATG 4 cut(s) 673, 829, 918, 1088
Fnu4HI GCNGC 5 cut(s) 10, 442, 455, 645, 879
FokI GGATG 3 cut(s) 217, 388, 749
Fsp4HI GCNGC 5 cut(s) 10, 442, 455, 645, 879
FspBI CTAG 5 cut(s) 161, 246, 327, 485, 867
GlaI GCGC 2 cut(s) 174, 335
GluI GCNGC 5 cut(s) 10, 442, 455, 645, 879
HaeIII GGCC 3 cut(s) 88, 265, 945
HapII CCGG 4 cut(s) 176, 535, 860, 1130
HhaI GCGC 2 cut(s) 175, 336
Hin1II CATG 4 cut(s) 677, 833, 922, 1092
Hin6I GCGC 2 cut(s) 173, 334
HinP1I GCGC 2 cut(s) 173, 334
HinfI GANTC 4 cut(s) 352, 769, 863, 1176
HpaII CCGG 4 cut(s) 176, 535, 860, 1130
HphI GGTGA 1 cut(s) 145
Hpy166II GTNNAC 4 cut(s) 43, 74, 823, 895
Hpy188I TCNGA 5 cut(s) 67, 416, 817, 928, 1181
Hpy188III TCNNGA 4 cut(s) 485, 535, 566, 593
Hpy8I GTNNAC 4 cut(s) 43, 74, 823, 895
HpyAV CCTTC 5 cut(s) 28, 590, 789, 822, 953
HpyCH4III ACNGT 4 cut(s) 26, 319, 1027, 1060
HpyCH4IV ACGT 3 cut(s) 45, 297, 480
HpyF10VI GCNNNNNNNGC 2 cut(s) 630, 1100
HpyF3I CTNAG 4 cut(s) 363, 712, 722, 1178
HpySE526I ACGT 3 cut(s) 45, 297, 480
Hsp92II CATG 4 cut(s) 677, 833, 922, 1092
HspAI GCGC 2 cut(s) 173, 334
KflI GGGWCCC 2 cut(s) 408, 568
Kpn2I TCCGGA 1 cut(s) 534
Kzo9I GATC 4 cut(s) 282, 388, 559, 808
LmnI GCTCC 1 cut(s) 17
Lsp1109I GCAGC 5 cut(s) 21, 441, 453, 631, 865
LweI GCATC 3 cut(s) 727, 865, 890
MaeI CTAG 5 cut(s) 161, 246, 327, 485, 867
MaeII ACGT 3 cut(s) 45, 297, 480
MaeIII GTNAC 1 cut(s) 669
MalI GATC 4 cut(s) 284, 390, 561, 810
MboI GATC 4 cut(s) 282, 388, 559, 808
MboII GAAGA 5 cut(s) 514, 626, 863, 980, 983
MfeI CAATTG 1 cut(s) 756
MflI RGATCY 2 cut(s) 388, 808
MluCI AATT 3 cut(s) 528, 756, 1000
MlyI GAGTC 2 cut(s) 872, 1170
MmeI TCCRAC 1 cut(s) 1150
Mph1103I ATGCAT 1 cut(s) 799
MroI TCCGGA 1 cut(s) 534
MroXI GAANNNNTTC 1 cut(s) 528
MseI TTAA 6 cut(s) 59, 423, 435, 548, 1068, 1152
MslI CAYNNNNRTG 1 cut(s) 828
MspCI CTTAAG 1 cut(s) 434
MspI CCGG 4 cut(s) 176, 535, 860, 1130
MspR9I CCNGG 1 cut(s) 860
MunI CAATTG 1 cut(s) 756
MwoI GCNNNNNNNGC 2 cut(s) 630, 1100
NciI CCSGG 1 cut(s) 860
NdeII GATC 4 cut(s) 282, 388, 559, 808
NlaIII CATG 4 cut(s) 677, 833, 922, 1092
NlaIV GGNNCC 7 cut(s) 89, 409, 410, 476, 569, 570, 1134
NmuCI GTSAC 1 cut(s) 669
NsiI ATGCAT 1 cut(s) 799
NspI RCATGY 2 cut(s) 677, 922
PaeR7I CTCGAG 1 cut(s) 939
PciI ACATGT 1 cut(s) 673
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 528
PfeI GAWTC 2 cut(s) 352, 769
PfoI TCCNGGA 1 cut(s) 858
PinAI ACCGGT 1 cut(s) 1129
PkrI GCNGC 5 cut(s) 11, 443, 456, 646, 880
PleI GAGTC 2 cut(s) 871, 1170
PpsI GAGTC 2 cut(s) 871, 1170
PpuMI RGGWCCY 2 cut(s) 408, 568
PscI ACATGT 1 cut(s) 673
Psp5II RGGWCCY 2 cut(s) 408, 568
PspN4I GGNNCC 7 cut(s) 89, 409, 410, 476, 569, 570, 1134
PspPI GGNCC 4 cut(s) 87, 264, 408, 568
PspPPI RGGWCCY 2 cut(s) 408, 568
PspXI VCTCGAGB 1 cut(s) 939
PsrI GAACNNNNNNTAC 2 cut(s) 102, 134
PstI CTGCAG 1 cut(s) 11
PstNI CAGNNNCTG 1 cut(s) 878
PsuI RGATCY 2 cut(s) 388, 808
RsaI GTAC 2 cut(s) 380, 822
RsaNI GTAC 2 cut(s) 379, 821
RseI CAYNNNNRTG 1 cut(s) 828
SaqAI TTAA 6 cut(s) 59, 423, 435, 548, 1068, 1152
SatI GCNGC 5 cut(s) 10, 442, 455, 645, 879
Sau3AI GATC 4 cut(s) 282, 388, 559, 808
Sau96I GGNCC 4 cut(s) 87, 264, 408, 568
SchI GAGTC 2 cut(s) 872, 1170
ScrFI CCNGG 1 cut(s) 860
SfaNI GCATC 3 cut(s) 727, 865, 890
SfcI CTRYAG 1 cut(s) 7
Sfr274I CTCGAG 1 cut(s) 939
SgrAI CRCCGGYG 1 cut(s) 175
SinI GGWCC 2 cut(s) 408, 568
SlaI CTCGAG 1 cut(s) 939
SmiMI CAYNNNNRTG 1 cut(s) 828
SmlI CTYRAG 3 cut(s) 434, 908, 939
SmoI CTYRAG 3 cut(s) 434, 908, 939
Sse9I AATT 3 cut(s) 528, 756, 1000
SsiI CCGC 1 cut(s) 1107
SspMI CTAG 5 cut(s) 161, 246, 327, 485, 867
StyD4I CCNGG 1 cut(s) 858
StyI CCWWGG 1 cut(s) 686
TaaI ACNGT 4 cut(s) 26, 319, 1027, 1060
TaiI ACGT 3 cut(s) 48, 300, 483
TaqI TCGA 1 cut(s) 940
TasI AATT 3 cut(s) 528, 756, 1000
TatI WGTACW 2 cut(s) 378, 820
TfiI GAWTC 2 cut(s) 352, 769
Tru1I TTAA 6 cut(s) 59, 423, 435, 548, 1068, 1152
Tru9I TTAA 6 cut(s) 59, 423, 435, 548, 1068, 1152
TscAI CASTG 2 cut(s) 151, 1032
TseFI GTSAC 1 cut(s) 669
TseI GCWGC 5 cut(s) 9, 441, 454, 644, 878
Tsp45I GTSAC 1 cut(s) 669
TspDTI ATGAA 7 cut(s) 17, 464, 481, 537, 627, 818, 972
TspRI CASTG 2 cut(s) 151, 1032
Vha464I CTTAAG 1 cut(s) 434
VpaK11BI GGWCC 2 cut(s) 408, 568
XagI CCTNNNNNAGG 1 cut(s) 957
XapI RAATTY 1 cut(s) 1000
XbaI TCTAGA 1 cut(s) 484
XceI RCATGY 2 cut(s) 677, 922
XhoI CTCGAG 1 cut(s) 939
XmnI GAANNNNTTC 1 cut(s) 528
XspI CTAG 5 cut(s) 161, 246, 327, 485, 867
Zsp2I ATGCAT 1 cut(s) 799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.